LOCUS       V015317                 3840 bp    DNA     circular SYN 10-SEP-2020
DEFINITION  Exported.
ACCESSION   V015317
VERSION     V015317
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
            .
REFERENCE   1  (bases 1 to 3840)
  AUTHORS   Groseclose TM, Rondon RE, Herde ZD, Aldrete CA, Wilson CJ.
  TITLE     Engineered systems of inducible anti-repressors for the next
            generation of biological programming
  JOURNAL   Nat Commun 11 (1), 4440 (2020)
   PUBMED   32895374
REFERENCE   2  (bases 1 to 3840)
  AUTHORS   Groseclose TM, Rondon RE, Herde ZD, Aldrete CA, Wilson CJ.
  TITLE     Direct Submission
REFERENCE   3  (bases 1 to 3840)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     ##Assembly-Data-START##
            Sequencing Technology :: Sanger dideoxy sequencing
            ##Assembly-Data-END##
            SGRef: number: 1; type: "Journal Article"; journalName: "Nat
            Commun"; date: "2020"; volume: "11"; issue: "1"; pages: "4440"
            SGRef: number: 2; type: "Journal Article"; journalName: "Submitted
            (28-FEB-2020) Chemical "
FEATURES             Location/Qualifiers
     source          1..3840
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        596..673
                     /label="lacI promoter"
     CDS             674..1663
                     /gene="rbsR"
                     /label="Ribose operon repressor"
                     /note="Ribose operon repressor from Escherichia coli
                     (strain K12). Accession#: P0ACQ0"
     protein_bind    1796..1817
                     /label="CAP binding site"
                     /note="CAP binding activates transcription in the presence
                     of cAMP."
     rep_origin      2008..2552
                     /label="p15A ori"
                     /note="Plasmids containing the medium-copy-number p15A
                     origin of replication can be propagated in E. coli cells
                     that contain a second plasmid with the ColE1 origin."
     promoter        3078..3180
                     /label="cat promoter"
                     /note="promoter of the E. coli cat gene encoding
                     chloramphenicol acetyltransferase"
     CDS             3181..3837
                     /label="CmR"
                     /note="chloramphenicol acetyltransferase"
ORIGIN
        1 tttttttaag gcagttattg gtgcccttaa acgcctggtg ctacgcctga ataagtgata
       61 ataagcggat gaatggcaga aattcgaaag caaattcgac ccggtcgtcg gttcagggca
      121 gggtcgttaa atagccgctt atgtctattg ctggtttacc ggtttattga ctaccggaag
      181 cagtgtgacc gtgtgcttct caaatgcctg aggccagttt gctcaggctc tccccgtgga
      241 ggtaataatt gacgatatga tcatttattc tgcctcccag agcctgataa aaacggttag
      301 cgcttcgtta atacagatgt aggtgttcca cagggtagcc agcagcatcc tgcgatgcag
      361 atccggaaca taatggtgca gggcgcttgt ttcggcgtgg gtatggtggc aggccccgtg
      421 gccgggggac tgttgggcgc tgccggcacc tgtcctacga gttgcatgat aaagaagaca
      481 gtcataagtg cggcgacgac accattcctt gcggcggcgg tgctcaacgg cctcaaccta
      541 ctactgggct gcttcctaat gcaggagtcg cataagggag agcgtcgaga tcccggacac
      601 catcgaatgg cgcaaaacct ttcgcggtat ggcatgatag cgcccggaag agagtcaatt
      661 cagggtggtg aatgtggcta caatgaaaga tgttgcccgc ctggcgggcg tttctacctc
      721 aacagtttct cacgttatca ataaagatcg cttcgtcagt gaagcgatta ccgccaaagt
      781 tgaagcggcg attaaagaac tcaattacgc gccatcagct ctggcgcgta gcctcaaact
      841 caatcaaaca cataccattg gcatgttgat cactgccagt accaatcctt tctattcaga
      901 actggtgcgt ggcgttgaac gcagctgctt cgaacgcggt tatagtctcg tcctttgcaa
      961 taccgaaggc gatgaacagc ggatgaatcg caatctggaa acgctgatgc aaaaacgcgt
     1021 tgatggcttg ctgttactgt gcaccgaaac gcatcaacct tcgcgtgaaa tcatgcaacg
     1081 ttatccgaca gtgcctactg tgatgatgga ctgggctccg ttcgatggcg acagcgatct
     1141 tattcaggat aactcgttgc tgggcggaga cttagcaacg caatatctga tcgataaagg
     1201 tcatacccgt atcgcctgta ttaccggccc gctggataaa actccggcgc gcctgcggtt
     1261 ggaaggttat cgggcggcga tgaaacgtgc gggtctcaac attcctgatg gctatgaagt
     1321 cactggtgat tttgaattta acggcgggtt tgacgctatg cgccaactgc tatcacatcc
     1381 gctgcgtcct caggccgtct ttaccggaaa tgacgctatg gctgttggcg tttaccaggc
     1441 gttatatcag gcagagttac aggttccgca ggatatcgcg gtgattggct atgacgatat
     1501 cgaactggca agctttatga cgccaccatt aaccactatc caccaaccga aagatgaact
     1561 gggggagctg gcgattgatg tactcatcca tcggataacc cagccgaccc ttcagcaaca
     1621 acgattacaa cttactccga ttctgatgga acgcggttcg gcttagctgg tgaaaagaaa
     1681 aaccaccctg gcgcccaata cgcaaaccgc ctctccccgc gcgttggccg attcattaat
     1741 gcagctggca cgacaggttt cccgactgga aagcgggcag tgagcgcaac gcaattaatg
     1801 taagttagct cactcattag gcaccgggat ctcgaccgat gcccttgaga gccttcaacc
     1861 cagtcagctc cttccggtgg gcgcggggca tgactaacat gagaattaca acttatatcg
     1921 tatggggctg acttcaggtg ctacatttga agagataaat tgcactgaaa tctagaaata
     1981 ttttatctga ttaataagat gatcttcttg agatcgtttt ggtctgcgcg taatctcttg
     2041 ctctgaaaac gaaaaaaccg ccttgcaggg cggtttttcg aaggttctct gagctaccaa
     2101 ctctttgaac cgaggtaact ggcttggagg agcgcagtca ccaaaacttg tcctttcagt
     2161 ttagccttaa ccggcgcatg acttcaagac taactcctct aaatcaatta ccagtggctg
     2221 ctgccagtgg tgcttttgca tgtctttccg ggttggactc aagacgatag ttaccggata
     2281 aggcgcagcg gtcggactga acggggggtt cgtgcataca gtccagcttg gagcgaactg
     2341 cctacccgga actgagtgtc aggcgtggaa tgagacaaac gcggccataa cagcggaatg
     2401 acaccggtaa accgaaaggc aggaacagga gagcgcacga gggagccgcc aggggaaacg
     2461 cctggtatct ttatagtcct gtcgggtttc gccaccactg atttgagcgt cagatttcgt
     2521 gatgcttgtc aggggggcgg agcctatgga aaaacggctt tgccgcggcc ctctcacttc
     2581 cctgttaagt atcttcctgg catcttccag gaaatctccg ccccgttcgt aagccatttc
     2641 cgctcgccgc agtcgaacga ccgagcgtag cgagtcagtg agcgaggaag cggaatatat
     2701 cctgtatcac atattctgct gacgcaccgg tgcagccttt tttctcctgc cacatgaagc
     2761 acttcactga caccctcatc agtgccaaca tagtaagcca gtatacactc cgctagcgct
     2821 gatgtccggc ggtgcttttg ccgttacgca ccaccccgtc agtagctgaa caggagggac
     2881 agctgataga aacagaagcc actggagcac ctcaaaaaca ccatcataca ctaaatcagt
     2941 aagttggcag catcacccga cgcactttgc gccgaataaa tacctgtgac ggaagatcac
     3001 ttcgcagaat aaataaatcc tggtgtccct gttgataccg ggaagccctg ggccaacttt
     3061 tggcgaaaat gagacgttga tcggcacgta agaggttcca actttcacca taatgaaata
     3121 agatcactac cgggcgtatt ttttgagtta tcgagatttt caggagctaa ggaagctaaa
     3181 atggagaaaa aaatcactgg atataccacc gttgatatat cccaatggca tcgtaaagaa
     3241 cattttgagg catttcagtc agttgctcaa tgtacctata accagaccgt tcagctggat
     3301 attacggcct ttttaaagac cgtaaagaaa aataagcaca agttttatcc ggcctttatt
     3361 cacattcttg cccgcctgat gaatgctcat ccggaattcc gtatggcaat gaaagacggt
     3421 gagctggtga tatgggatag tgttcaccct tgttacaccg ttttccatga gcaaactgaa
     3481 acgttttcat cgctctggag tgaataccac gacgatttcc ggcagtttct acacatatat
     3541 tcgcaagatg tggcgtgtta cggtgaaaac ctggcctatt tccctaaagg gtttattgag
     3601 aatatgtttt tcgtctcagc caatccctgg gtgagtttca ccagttttga tttaaacgtg
     3661 gccaatatgg acaacttctt cgcccccgtt ttcaccatgg gcaaatatta tacgcaaggc
     3721 gacaaggtgc tgatgccgct ggcgattcag gttcatcatg ccgtctgtga tggcttccat
     3781 gtcggcagaa tgcttaatga attacaacag tactgcgatg agtggcaggg cggggcgtaa
//
