LOCUS       40924_36333        5312 bp DNA     circular SYN 18-DEC-2018
DEFINITION  Cloning vector pRama-35, complete sequence.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 5312)
  AUTHORS   Ganguly S, Liu J, Pillai VB, Mittler RS, Amara RR.
  TITLE     Adjuvantive effects of anti-4-1BB agonist Ab and 4-1BBL DNA for a 
            HIV-1 Gag DNA vaccine: different effects on cellular and humoral 
            immunity
  JOURNAL   Vaccine 28 (5), 1300-1309 (2010)
  PUBMED    19944789
REFERENCE   2  (bases 1 to 5312)
  AUTHORS   Pillai VB, Amara RR.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-JUN-2009) Microbiology and Immunology, Emory 
            University, 954 Gatewood Road NE, Atlanta, GA 30329, USA
REFERENCE   3  (bases 1 to 5312)
  TITLE     Direct Submission
REFERENCE   4  (bases 1 to 5312)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Vaccine"; 
            date: "2010"; volume: "28"; issue: "5"; pages: "1300-1309"
COMMENT     SGRef: number: 2; type: "Journal Article"; journalName: "Submitted 
            (10-JUN-2009) Microbiology and Immunology, Emory University, 954 
            Gatewood Road NE, Atlanta, GA 30329, USA"
COMMENT     SGRef: number: 3; type: "Journal Article"
FEATURES             Location/Qualifiers
     source          1..5312
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     enhancer        93..474
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        475..678
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early
                     promoter"
     intron          814..1640
                     /label=CMV intron A
                     /note="human cytomegalovirus intron A (Chapman et al.,
                     1991)"
     CDS             1784..3172
                     /codon_start=1
                     /product="SP-D-4-1BBL"
                     /label=SP-D-4-1BBL
                     /note="multimerization domain of lung surfactant protein D 
                     (SP-D) is fused to the extracellular domain of 4-1BBL"
                     /protein_id="ACT68289.1"
                     /translation="MLPFLSMLVLLVQPLGNLGAEMKSLSQRSVPNTCTLVMCSPTENG
                     LPGRDGRDGREGPRGEKGDPGLPGPMGLSGLQGPTGPVGPKGENGSAGEPGPKGERGLS
                     GPPGLPGIPGPAGKEGPSGKQGNIGPQGKPGPKGEAGPKGEVGAPGMQGSTGAKGSTGP
                     KGERGAPGVQGAPGNAGAAGPAGPAGPQGAPGSRGPPGLKGDRGVPGDRGIKGESGLPD
                     SAALRQQMEALKGKLQRLEVAFSHYQKAALFPDGRTEPRPALTITTSPNLGTRENNADQ
                     VTPVSHIGCPNTTQQGSPVFAKLLAKNQASLCNTTLNWHSQDGAGSSYLSQGLRYEEDK
                     KELVVDSPGLYYVFLELKLSPTFTNTGHKVQGWVSLVLQAKPQVDDFDNLALTVELFPC
                     SMENKLVDRSWSQLLLLKAGHRLSVGLRAYLHGAQDAYRDWELSYPNTTSFGLFLVKPD
                     NPWE"
     polyA_signal    3179..3401
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     terminator      complement(3402..3436)
                     /label=lambda t0 terminator
                     /note="minimal transcription terminator from phage lambda 
                     (Scholtissek and Grosse, 1987)"
     CDS             complement(3457..4248)
                     /codon_start=1
                     /label=NeoR/KanR
                     /note="aminoglycoside phosphotransferase"
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKASMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     rep_origin      4660..5248
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
ORIGIN
        1 cgacaatatt ggctattggc cattgcatac gttgtatcta tatcataata tgtacattta
       61 tattggctca tgtccaatat gaccgccatg ttgacattga ttattgacta gttattaata
      121 gtaatcaatt acgggttcat tagttcatag cccatatatg gagttccgcg ttacataact
      181 tacggtaaat ggcccgcctg gctgaccgcc caacgacccc cgcccattga cgtcaataat
      241 gacgtatgtt cccatagtaa cgccaatagg gactttccat tgacgtcaat gggtggagta
      301 tttacggtaa actgcccact tggcagtaca tcaagtgtat catatgccaa gtccgccccc
      361 tattgacgtc aatgacggta aatggcccgc ctggcattat gcccagtaca tgaccttacg
      421 ggactttcct acttggcagt acatctacgg tattagtcat cggctattac catggtgatg
      481 cggttttggc agtacaccaa tgggcgtgga tagcggtttg actcacgggg atttccaagt
      541 ctccacccca ttgacgtcaa tgggagtttg ttttggcacc aaaatcaacg ggactttcca
      601 aaatgtcgta ataaccccgc cccgttgacg caaatgggcg gtaggcgtgt acggtgggag
      661 gtctatataa gcagagctcg tttagtgaac cgtcagatcg cctggagacg ccatccacgc
      721 tgttttgacc tccatagaag acaccgggac cgatccagcc tccgcggccg ggaacggtgc
      781 attggaacgc ggattccccg tgccaagagt gacgtaagta ccgcctatag actctatagg
      841 cacacccctt tggctcttat gcatgctata ctgtttttgg cttggggcct atacaccccc
      901 gcttccttat gctataggtg atggtatagc ttagcctata ggtgtgggtt attgaccatt
      961 attgaccact cccctattgg tgacgatact ttccattact aatccataac atggctcttt
     1021 gccacaacta tctctattgg ctatatgcca atactctgtc cttcagagac tgacacggac
     1081 tctgtatttt tacaggatgg ggtcccattt attatttaca aattcacata tacaacaacg
     1141 ccgtcccccg tgcccgcagt ttttattaaa catagcgtgg gatctccacg cgaatctcgg
     1201 gtaccgtgtt ccggacatgg gytcttctcc ggtagcggcg gagcttccac atccgagccc
     1261 tggtcccatg cctccagcgg ctcatggtcg ctcggcagct ccttgctcct aacagtggag
     1321 gccagactta ggcacagcac aatgcccacc accaccagtg tgccgcacaa ggccgtggcg
     1381 gtagggtatg tgtctgaaaa tgagctcgga gattgggctc gcaccgctga cgcagatgga
     1441 agacttaagg cagcggcaga agaagatgca ggcagctgag ttgttgtatt ctgataagag
     1501 tcagaggtaa ctcccgttgc ggtgctgtta acggtggagg gcagtgtagt ctgagcagta
     1561 ctcgttgctg ccgcgcgcgc caccagacat aatagctgac agactaacag actgttcctt
     1621 tccatgggtc ttttctgcag tcaccatcga tgcttgcaat catggatgca atgaagagag
     1681 ggctctgctg tgtgctgctg ctgtgtggag aattcttcgt ttctgctgct gtgtggagaa
     1741 ttcttcgttt cggctagcga attccaccag gaagcaatct gacatgctgc cctttctctc
     1801 catgcttgtc ttgcttgtac agcccctggg aaatctggga gcagaaatga agagcctctc
     1861 gcagagatca gtacccaaca cctgcaccct agtcatgtgt agcccaacag agaatggcct
     1921 gcctggtcgt gatggacggg atgggagaga aggtccacgg ggtgagaagg gtgatccagg
     1981 tttgccagga cctatggggc tctcagggtt gcagggccct acaggtccag ttggacccaa
     2041 aggagagaat ggctctgctg gcgaacctgg accaaaggga gaacgtggac taagtggacc
     2101 tccaggactt ccaggtattc ctggtccagc tgggaaagaa ggtccctctg ggaagcaggg
     2161 gaacatagga cctcaaggca aaccaggtcc taaaggagag gctgggccca aaggagaagt
     2221 aggtgctcct ggcatgcaag gatctacagg ggcaaaaggc tccacaggcc ccaagggaga
     2281 aagaggtgcc cctggtgtgc aaggagcccc agggaatgct ggagcagcag gacctgccgg
     2341 acctgccggt ccacagggag ctccaggttc cagggggccc ccaggactca agggggacag
     2401 aggtgttcct ggagacagag gaatcaaagg tgaaagcggg cttccagaca gtgctgctct
     2461 gaggcagcag atggaggcct taaaaggaaa actacagcgt ctagaggttg ccttctccca
     2521 ctatcagaaa gctgcattgt tccctgatgg ccgcaccgag cctcggccag cgctcacaat
     2581 caccacctcg cccaacctgg gtacccgaga gaataatgca gaccaggtca cccctgtttc
     2641 ccacattggc tgccccaaca ctacacaaca gggctctcct gtgttcgcca agctactggc
     2701 taaaaaccaa gcatcgttgt gcaatacaac tctgaactgg cacagccaag atggagctgg
     2761 gagctcatac ctatctcaag gtctgaggta cgaagaagac aaaaaggagt tggtggtaga
     2821 cagtcccggg ctctactacg tatttttgga actgaagctc agtccaacat tcacaaacac
     2881 aggccacaag gtgcagggct gggtctctct tgttttgcaa gcaaagcctc aggtagatga
     2941 ctttgacaac ttggccctga cagtggaact gttcccttgc tccatggaga acaagttagt
     3001 ggaccgttcc tggagtcaac tgttgctcct gaaggctggc caccgcctca gtgtgggtct
     3061 gagggcttat ctgcatggag cccaggatgc atacagagac tgggagctgt cttatcccaa
     3121 caccaccagc tttggactct ttcttgtgaa acccgacaac ccatgggaat gacctaggct
     3181 gtgccttcta gttgccagcc atctgttgtt tgcccctccc ccgtgccttc cttgaccctg
     3241 gaaggtgcca ctcccactgt cctttcctaa taaaatgagg aaattgcatc gcattgtctg
     3301 agtaggtgtc attctattct ggggggtggg gtggggcagg acagcaaggg ggaggattgg
     3361 gaagacaata gcaggcatgc tggggatgcg gtgggctcta tataaaaaac gcccggcggc
     3421 aaccgagcgt tctgaacgct agagtcgatc gattcagaag aactcgtcaa gaaggcgata
     3481 gaaggcgatg cgctgcgaat cgggagcggc gataccgtaa agcacgagga agcggtcagc
     3541 ccattcgccg ccaagctctt cagcaatatc acgggtagcc aacgctatgt cctgatagcg
     3601 gtctgccaca cccagccggc cacagtcgat gaatccagaa aagcggccat tttccaccat
     3661 gatattcggc aagcaggcat cgccatgggt cacgacgaga tcctcgccgt cgggcatgct
     3721 cgccttgagc ctggcgaaca gttcggctgg cgcgagcccc tgatgctctt cgtccagatc
     3781 atcctgatcg acaagaccgg cttccatccg agtacgtgct cgctcgatgc gatgtttcgc
     3841 ttggtggtcg aatgggcagg tagccggatc aagcgtatgc agccgccgca ttgcatcagc
     3901 catgatggat actttctcgg caggagcaag gtgagatgac aggagatcct gccccggcac
     3961 ttcgcccaat agcagccagt cccttcccgc ttcagtgaca acgtcgagca cagctgcgca
     4021 aggaacgccc gtcgtggcca gccacgatag ccgcgctgcc tcgtcttgca gttcattcag
     4081 ggcaccggac aggtcggtct tgacaaaaag aaccgggcgc ccctgcgctg acagccggaa
     4141 cacggcggca tcagagcagc cgattgtctg ttgtgcccag tcatagccga atagcctctc
     4201 cacccaagcg gccggagaac ctgcgtgcaa tccatcttgt tcaatcatgc gaaacgatcc
     4261 tcatcctgtc tcttgatcag atcttgatcc cctgcgccat cagatccttg gcggcaagaa
     4321 agccatccag tttactttgc agggcttccc aaccttacca gagggcgccc cagctggcaa
     4381 ttccggttcg cttgctgtcc ataaaaccgc ccagtctagc tatcgccatg taagcccact
     4441 gcaagctacc tgctttctct ttgcgcttgc gttttccctt gtccagatag cccagtagct
     4501 gacattcatc cggggtcagc accgtttctg cggactggct ttctacgtga aaaggatcta
     4561 ggtgaagatc ctttttgata atctcatgac caaaatccct taacgtgagt tttcgttcca
     4621 ctgagcgtca gaccccgtag aaaagatcaa aggatcttct tgagatcctt tttttctgcg
     4681 cgtaatctgc tgcttgcaaa caaaaaaacc accgctacca gcggtggttt gtttgccgga
     4741 tcaagagcta ccaactcttt ttccgaaggt aactggcttc agcagagcgc agataccaaa
     4801 tactgttctt ctagtgtagc cgtagttagg ccaccacttc aagaactctg tagcaccgcc
     4861 tacatacctc gctctgctaa tcctgttacc agtggctgct gccagtggcg ataagtcgtg
     4921 tcttaccggg ttggactcaa gacgatagtt accggataag gcgcagcggt cgggctgaac
     4981 ggggggttcg tgcacacagc ccagcttgga gcgaacgacc tacaccgaac tgagatacct
     5041 acagcgtgag ctatgagaaa gcgccacgct tcccgaaggg agaaaggcgg acaggtatcc
     5101 ggtaagcggc agggtcggaa caggagagcg cacgagggag cttccagggg gaaacgcctg
     5161 gtatctttat agtcctgtcg ggtttcgcca cctctgactt gagcgtcgat ttttgtgatg
     5221 ctcgtcaggg gggcggagcc tatggaaaaa cgccagcaac gcggccgctt tacggttcct
     5281 ggccttttgc tggccttttg ctcacatgtt gt
//
