LOCUS       V003830                 8361 bp    DNA     circular SYN 18-DEC-2018
DEFINITION  Exported.
ACCESSION   V003830
VERSION     V003830
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
            .
REFERENCE   1  (bases 1 to 8361)
  AUTHORS   Ganguly S, Liu J, Pillai VB, Mittler RS, Amara RR.
  TITLE     Adjuvantive effects of anti-4-1BB agonist Ab and 4-1BBL DNA for a
            HIV-1 Gag DNA vaccine: different effects on cellular and humoral
            immunity
  JOURNAL   Vaccine 28 (5), 1300-1309 (2010)
   PUBMED   19944789
REFERENCE   2  (bases 1 to 8361)
  AUTHORS   Ganguly S, Amara RR.
  TITLE     Direct Submission
  JOURNAL   Submitted (08-JUN-2009) Microbiology and Immunology, Emory
            University, 954 Gatewood Road NE, Atlanta, GA 30329, USA
REFERENCE   3  (bases 1 to 8361)
  TITLE     Direct Submission
REFERENCE   4  (bases 1 to 8361)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Vaccine";
            date: "2010"; volume: "28"; issue: "5"; pages: "1300-1309"
            SGRef: number: 2; type: "Journal Article"; journalName: "Submitted
            (08-JUN-2009) Microbiology and Immunology, Emory University, 954
            Gatewood Road NE, Atlanta, GA 30329, USA"
            SGRef: number: 3; type: "Journal Article"
FEATURES             Location/Qualifiers
     source          1..8361
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     enhancer        93..472
                     /label="CMV enhancer"
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        476..675
                     /label="CMV promoter"
                     /note="human cytomegalovirus (CMV) immediate early
                     promoter"
     intron          814..1640
                     /label="CMV intron A"
                     /note="human cytomegalovirus intron A (Chapman et al.,
                     1991)"
     CDS             1650..3182
                     /gene="gag"
                     /label="Gag polyprotein"
                     /note="Gag polyprotein from Human immunodeficiency virus
                     type 1 group M subtype B (isolate BH10). Accession#:
                     P03347"
     polyA_signal    3225..3447
                     /label="bGH poly(A) signal"
                     /note="bovine growth hormone polyadenylation signal"
     regulatory      complement(3445..3479)
                     /label="Lambda To terminator"
                     /note="Lambda To terminator"
                     /regulatory_class="terminator"
     terminator      3448..3481
                     /label="lambda t0 terminator"
                     /note="minimal transcription terminator from phage lambda
                     (Scholtissek and Grosse, 1987)"
     enhancer        3626..4007
                     /label="CMV enhancer"
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        4008..4211
                     /label="CMV promoter"
                     /note="human cytomegalovirus (CMV) immediate early
                     promoter"
     intron          4224..5171
                     /number=A
                     /note="CMV intron A"
     intron          4347..5173
                     /label="CMV intron A"
                     /note="human cytomegalovirus intron A (Chapman et al.,
                     1991)"
     CDS             5292..6218
                     /gene="Tnfsf9"
                     /label="Tumor necrosis factor ligand superfamily member 9"
                     /note="Tumor necrosis factor ligand superfamily member 9
                     from Mus musculus. Accession#: P41274"
     regulatory      6225..6447
                     /label="bovine growth hormone polyadenylation signal"
                     /note="bovine growth hormone polyadenylation signal"
                     /regulatory_class="polyA_signal_sequence"
     polyA_signal    6228..6435
                     /label="bGH poly(A) signal"
                     /note="bovine growth hormone polyadenylation signal"
     terminator      complement(6451..6485)
                     /label="lambda t0 terminator"
                     /note="minimal transcription terminator from phage lambda
                     (Scholtissek and Grosse, 1987)"
     CDS             complement(6506..7297)
                     /label="NeoR/KanR"
                     /note="aminoglycoside phosphotransferase"
     rep_origin      7709..8297
                     /direction=RIGHT
                     /label="ori"
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
                     replication"
ORIGIN
        1 cgacaatatt ggctattggc cattgcatac gttgtatcta tatcataata tgtacattta
       61 tattggctca tgtccaatat gaccgccatg ttgacattga ttattgacta gttattaata
      121 gtaatcaatt acgggttcat tagttcatag cccatatatg gagttccgcg ttacataact
      181 tacggtaaat ggcccgcctg gctgaccgcc caacgacccc cgcccattga cgtcaataat
      241 gacgtatgtt cccatagtaa cgccaatagg gactttccat tgacgtcaat gggtggagta
      301 tttacggtaa actgcccact tggcagtaca tcaagtgtat catatgccaa gtccgccccc
      361 tattgacgtc aatgacggta aatggcccgc ctggcattat gcccagtaca tgaccttacg
      421 ggactttcct acttggcagt acatctacgg tattagtcat cggctattac catggtgatg
      481 cggttttggc agtacaccaa tgggcgtgga tagcggtttg actcacgggg atttccaagt
      541 ctccacccca ttgacgtcaa tgggagtttg ttttggcacc aaaatcaacg ggactttcca
      601 aaatgtcgta ataaccccgc cccgttgacg caaatgggcg gtaggcgtgt acggtgggag
      661 gtctatataa gcagagctcg tttagtgaac cgtcagatcg cctggagacg ccatccacgc
      721 tgttttgacc tccatagaag acaccgggac cgatccagcc tccgcggccg ggaacggtgc
      781 attggaacgc ggattccccg tgccaagagt gacgtaagta ccgcctatag actctatagg
      841 cacacccctt tggctcttat gcatgctata ctgtttttgg cttggggcct atacaccccc
      901 gcttccttat gctataggtg atggtatagc ttagcctata ggtgtgggtt attgaccatt
      961 attgaccact cccctattgg tgacgatact ttccattact aatccataac atggctcttt
     1021 gccacaacta tctctattgg ctatatgcca atactctgtc cttcagagac tgacacggac
     1081 tctgtatttt tacaggatgg ggtcccattt attatttaca aattcacata tacaacaacg
     1141 ccgtcccccg tgcccgcagt ttttattaaa catagcgtgg gatctccacg cgaatctcgg
     1201 gtaccgtgtt ccggacatgg gytcttctcc ggtagcggcg gagcttccac atccgagccc
     1261 tggtcccatg cctccagcgg ctcatggtcg ctcggcagct ccttgctcct aacagtggag
     1321 gccagactta ggcacagcac aatgcccacc accaccagtg tgccgcacaa ggccgtggcg
     1381 gtagggtatg tgtctgaaaa tgagctcgga gattgggctc gcaccgctga cgcagatgga
     1441 agacttaagg cagcggcaga agaagatgca ggcagctgag ttgttgtatt ctgataagag
     1501 tcagaggtaa ctcccgttgc ggtgctgtta acggtggagg gcagtgtagt ctgagcagta
     1561 ctcgttgctg ccgcgcgcgc caccagacat aatagctgac agactaacag actgttcctt
     1621 tccatgggtc ttttctgcag tcaccatcga tgggcgcccg cgcctccgtg ctgtccggcg
     1681 gcgagctgga ccgctgggag aagatccgcc tgcgccccgg cggcaagaag aagtacaagc
     1741 tgaagcacat cgtgtgggcc tcccgcgagc tggagcgctt cgccgtgaac cccggcctgc
     1801 tggagacctc cgagggctgc cgccagatcc tgggccagct gcagccctcc ctgcagaccg
     1861 gctccgagga gctgcgctcc ctgtacaaca ccgtggccac cctgtactgc gtgcaccagc
     1921 gcatcgagat caaggacacc aaggaggccc tggacaagat cgaggaggag cagaacaagt
     1981 ccaagaagaa ggcccagcag gccgccgccg acaccggcca ctcctcccag gtgtcccaga
     2041 actaccccat cgtgcagaac atccagggcc agatggtgca ccaggccatc tccccccgca
     2101 ccctgaacgc ctgggtgaag gtggtggagg agaaggcctt ctcccccgag gtgatcccca
     2161 tgttctccgc cctgtccgag ggcgccaccc cccaggacct gaacaccatg ctgaacaccg
     2221 tgggcggcca ccaggccgcc atgcagatgc tgaaggagac catcaacgag gaggccgccg
     2281 agtgggaccg cgtgcacccc gtgcacgccg gccccatcgc ccccggccag atgcgcgagc
     2341 cccgcggctc cgacatcgcc ggcaccacct ccaccctgca ggagcagatc ggctggatga
     2401 ccaacaaccc ccccatcccc gtgggcgaga tctacaagcg ctggatcatc ctgggcctga
     2461 acaagatcgt gcgcatgtac tcccccacct ccatcctgga catccgccaa ggccccaagg
     2521 agcccttccg cgactacgtg gaccgcttct acaagaccct gcgcgccgag caggcctccc
     2581 aggaggtgaa gaactggatg accgagaccc tgctggtgca gaacgccaac cccgactgca
     2641 agaccatcct gaaggccctg ggccccgccg ccaccctgga ggagatgatg accgcctgcc
     2701 agggcgtggg cggccccggc cacaaggccc gcgtgctggc cgaggccatg tcccaggtga
     2761 ccaacaccgc caccatcatg atgcagcgcg gcaacttccg caaccagcgc aagatggtga
     2821 agtccttcaa ctccggcaag gagggccaca ccgcccgcaa ctgccgcgcc ccccgcaaga
     2881 agggctcctg gaagtccggc aaggagggcc accagatgaa ggactgcacc gagagacagg
     2941 ctaatttttt agggaagatc tggccttcct acaagggaag gccagggaat tttcttcaga
     3001 gcagaccaga gccaacagcc ccaccatttc ttcagagcag accagagcca acagccccac
     3061 cagaagagag cttcaggtct ggggtagaga caacaactcc ccctcagaag caggagccga
     3121 tagacaagga actgtatcct ttaacttccc tcagatcact ctttggcaac gacccctcgt
     3181 cttaagctag ccccgggtga taaacggacc gcgcaatccc taggctgtgc cttctagttg
     3241 ccagccatct gttgtttgcc cctcccccgt gccttccttg accctggaag gtgccactcc
     3301 cactgtcctt tcctaataaa atgaggaaat tgcatcgcat tgtctgagta ggtgtcattc
     3361 tattctgggg ggtggggtgg ggcaggacag caagggggag gattgggaag acaatagcag
     3421 gcatgctggg gatgcggtgg gctctatata aaaaacgccc ggcggcaacc gagcgttctg
     3481 agcggccgct ttacggttcc tggccttttg ctggcctttt gctcacatgt tgtcgacaat
     3541 attggctatt ggccattgca tacgttgtat ctatatcata atatgtacat ttatattggc
     3601 tcatgtccaa tatgaccgcc atgttgacat tgattattga ctagttatta atagtaatca
     3661 attacgggtt cattagttca tagcccatat atggagttcc gcgttacata acttacggta
     3721 aatggcccgc ctggctgacc gcccaacgac ccccgcccat tgacgtcaat aatgacgtat
     3781 gttcccatag taacgccaat agggactttc cattgacgtc aatgggtgga gtatttacgg
     3841 taaactgccc acttggcagt acatcaagtg tatcatatgc caagtccgcc ccctattgac
     3901 gtcaatgacg gtaaatggcc cgcctggcat tatgcccagt acatgacctt acgggacttt
     3961 cctacttggc agtacatcta cggtattagt catcggctat taccatggtg atgcggtttt
     4021 ggcagtacac caatgggcgt ggatagcggt ttgactcacg gggatttcca agtctccacc
     4081 ccattgacgt caatgggagt ttgttttggc accaaaatca acgggacttt ccaaaatgtc
     4141 gtaataaccc cgccccgttg acgcaaatgg gcggtaggcg tgtacggtgg gaggtctata
     4201 taagcagagc tcgtttagtg aaccgtcaga tcgcctggag acgccatcca cgctgttttg
     4261 acctccatag aagacaccgg gaccgatcca gcctccgcgg ccgggaacgg tgcattggaa
     4321 cgcggattcc ccgtgccaag agtgacgtaa gtaccgccta tagactctat aggcacaccc
     4381 ctttggctct tatgcatgct atactgtttt tggcttgggg cctatacacc cccgcttcct
     4441 tatgctatag gtgatggtat agcttagcct ataggtgtgg gttattgacc attattgacc
     4501 actcccctat tggtgacgat actttccatt actaatccat aacatggctc tttgccacaa
     4561 ctatctctat tggctatatg ccaatactct gtccttcaga gactgacacg gactctgtat
     4621 ttttacagga tggggtccca tttattattt acaaattcac atatacaaca acgccgtccc
     4681 ccgtgcccgc agtttttatt aaacatagcg tgggatctcc acgcgaatct cgggtaccgt
     4741 gttccggaca tgggytcttc tccggtagcg gcggagcttc cacatccgag ccctggtccc
     4801 atgcctccag cggctcatgg tcgctcggca gctccttgct cctaacagtg gaggccagac
     4861 ttaggcacag cacaatgccc accaccacca gtgtgccgca caaggccgtg gcggtagggt
     4921 atgtgtctga aaatgagctc ggagattggg ctcgcaccgc tgacgcagat ggaagactta
     4981 aggcagcggc agaagaagat gcaggcagct gagttgttgt attctgataa gagtcagagg
     5041 taactcccgt tgcggtgctg ttaacggtgg agggcagtgt agtctgagca gtactcgttg
     5101 ctgccgcgcg cgccaccaga cataatagct gacagactaa cagactgttc ctttccatgg
     5161 gtcttttctg cagtcaccat cgatgcttgc aatcatggat gcaatgaaga gagggctctg
     5221 ctgtgtgctg ctgctgtgtg gagaattctt cgtttctgct gctgtgtgga gaattcttcg
     5281 tttcggctag catggaccag cacacacttg atgtggagga taccgcggat gccagacatc
     5341 cagcaggtac ttcgtgcccc tcggatgcgg cgctcctcag agataccggg ctcctcgcgg
     5401 acgctgcgct cctctcagat actgtgcgcc ccacaaatgc cgcgctcccc acggatgctg
     5461 cctaccctgc ggttaatgtt cgggatcgcg aggccgcgtg gccgcctgca ctgaacttct
     5521 gttcccgcca cccaaagctc tatggcctag tcgctttggt tttgctgctt ctgatcgccg
     5581 cctgtgttcc tatcttcacc cgcaccgagc ctcggccagc gctcacaatc accacctcgc
     5641 ccaacctggg tacccgagag aataatgcag accaggtcac ccctgtttcc cacattggct
     5701 gccccaacac tacacaacag ggctctcctg tgttcgccaa gctactggct aaaaaccaag
     5761 catcgttgtg caatacaact ctgaactggc acagccaaga tggagctggg agctcatacc
     5821 tatctcaagg tctgaggtac gaagaagaca aaaaggagtt ggtggtagac agtcccgggc
     5881 tctactacgt atttttggaa ctgaagctca gtccaacatt cacaaacaca ggccacaagg
     5941 tgcagggctg ggtctctctt gttttgcaag caaagcctca ggtagatgac tttgacaact
     6001 tggccctgac agtggaactg ttcccttgct ccatggagaa caagttagtg gaccgttcct
     6061 ggagtcaact gttgctcctg aaggctggcc accgcctcag tgtgggtctg agggcttatc
     6121 tgcatggagc ccaggatgca tacagagact gggagctgtc ttatcccaac accaccagct
     6181 ttggactctt tcttgtgaaa cccgacaacc catgggaatg acctaggctg tgccttctag
     6241 ttgccagcca tctgttgttt gcccctcccc cgtgccttcc ttgaccctgg aaggtgccac
     6301 tcccactgtc ctttcctaat aaaatgagga aattgcatcg cattgtctga gtaggtgtca
     6361 ttctattctg gggggtgggg tggggcagga cagcaagggg gaggattggg aagacaatag
     6421 caggcatgct ggggatgcgg tgggctctat ataaaaaacg cccggcggca accgagcgtt
     6481 ctgaacgcta gagtcgatcg attcagaaga actcgtcaag aaggcgatag aaggcgatgc
     6541 gctgcgaatc gggagcggcg ataccgtaaa gcacgaggaa gcggtcagcc cattcgccgc
     6601 caagctcttc agcaatatca cgggtagcca acgctatgtc ctgatagcgg tctgccacac
     6661 ccagccggcc acagtcgatg aatccagaaa agcggccatt ttccaccatg atattcggca
     6721 agcaggcatc gccatgggtc acgacgagat cctcgccgtc gggcatgctc gccttgagcc
     6781 tggcgaacag ttcggctggc gcgagcccct gatgctcttc gtccagatca tcctgatcga
     6841 caagaccggc ttccatccga gtacgtgctc gctcgatgcg atgtttcgct tggtggtcga
     6901 atgggcaggt agccggatca agcgtatgca gccgccgcat tgcatcagcc atgatggata
     6961 ctttctcggc aggagcaagg tgagatgaca ggagatcctg ccccggcact tcgcccaata
     7021 gcagccagtc ccttcccgct tcagtgacaa cgtcgagcac agctgcgcaa ggaacgcccg
     7081 tcgtggccag ccacgatagc cgcgctgcct cgtcttgcag ttcattcagg gcaccggaca
     7141 ggtcggtctt gacaaaaaga accgggcgcc cctgcgctga cagccggaac acggcggcat
     7201 cagagcagcc gattgtctgt tgtgcccagt catagccgaa tagcctctcc acccaagcgg
     7261 ccggagaacc tgcgtgcaat ccatcttgtt caatcatgcg aaacgatcct catcctgtct
     7321 cttgatcaga tcttgatccc ctgcgccatc agatccttgg cggcaagaaa gccatccagt
     7381 ttactttgca gggcttccca accttaccag agggcgcccc agctggcaat tccggttcgc
     7441 ttgctgtcca taaaaccgcc cagtctagct atcgccatgt aagcccactg caagctacct
     7501 gctttctctt tgcgcttgcg ttttcccttg tccagatagc ccagtagctg acattcatcc
     7561 ggggtcagca ccgtttctgc ggactggctt tctacgtgaa aaggatctag gtgaagatcc
     7621 tttttgataa tctcatgacc aaaatccctt aacgtgagtt ttcgttccac tgagcgtcag
     7681 accccgtaga aaagatcaaa ggatcttctt gagatccttt ttttctgcgc gtaatctgct
     7741 gcttgcaaac aaaaaaacca ccgctaccag cggtggtttg tttgccggat caagagctac
     7801 caactctttt tccgaaggta actggcttca gcagagcgca gataccaaat actgttcttc
     7861 tagtgtagcc gtagttaggc caccacttca agaactctgt agcaccgcct acatacctcg
     7921 ctctgctaat cctgttacca gtggctgctg ccagtggcga taagtcgtgt cttaccgggt
     7981 tggactcaag acgatagtta ccggataagg cgcagcggtc gggctgaacg gggggttcgt
     8041 gcacacagcc cagcttggag cgaacgacct acaccgaact gagataccta cagcgtgagc
     8101 tatgagaaag cgccacgctt cccgaaggga gaaaggcgga caggtatccg gtaagcggca
     8161 gggtcggaac aggagagcgc acgagggagc ttccaggggg aaacgcctgg tatctttata
     8221 gtcctgtcgg gtttcgccac ctctgacttg agcgtcgatt tttgtgatgc tcgtcagggg
     8281 ggcggagcct atggaaaaac gccagcaacg cggccctttt acggttcctg gccttttgct
     8341 ggccttttgc tcacatgttg t
//
