LOCUS       V003831                 4828 bp    DNA     circular SYN 18-DEC-2018
DEFINITION  Exported.
ACCESSION   V003831
VERSION     V003831
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
            .
REFERENCE   1  (bases 1 to 4828)
  AUTHORS   Ganguly S, Liu J, Pillai VB, Mittler RS, Amara RR.
  TITLE     Adjuvantive effects of anti-4-1BB agonist Ab and 4-1BBL DNA for a
            HIV-1 Gag DNA vaccine: different effects on cellular and humoral
            immunity
  JOURNAL   Vaccine 28 (5), 1300-1309 (2010)
   PUBMED   19944789
REFERENCE   2  (bases 1 to 4828)
  AUTHORS   Ganguly S, Amara RR.
  TITLE     Direct Submission
  JOURNAL   Submitted (08-JUN-2009) Microbiology and Immunology, Emory
            University, 954 Gatewood Road NE, Atlanta, GA 30329, USA
REFERENCE   3  (bases 1 to 4828)
  TITLE     Direct Submission
REFERENCE   4  (bases 1 to 4828)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Vaccine";
            date: "2010"; volume: "28"; issue: "5"; pages: "1300-1309"
            SGRef: number: 2; type: "Journal Article"; journalName: "Submitted
            (08-JUN-2009) Microbiology and Immunology, Emory University, 954
            Gatewood Road NE, Atlanta, GA 30329, USA"
            SGRef: number: 3; type: "Journal Article"
FEATURES             Location/Qualifiers
     source          1..4828
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     enhancer        93..474
                     /label="CMV enhancer"
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        475..678
                     /label="CMV promoter"
                     /note="human cytomegalovirus (CMV) immediate early
                     promoter"
     intron          814..1640
                     /label="CMV intron A"
                     /note="human cytomegalovirus intron A (Chapman et al.,
                     1991)"
     CDS             1759..2685
                     /gene="Tnfsf9"
                     /label="Tumor necrosis factor ligand superfamily member 9"
                     /note="Tumor necrosis factor ligand superfamily member 9
                     from Mus musculus. Accession#: P41274"
     polyA_signal    2695..2917
                     /label="bGH poly(A) signal"
                     /note="bovine growth hormone polyadenylation signal"
     terminator      complement(2918..2952)
                     /label="lambda t0 terminator"
                     /note="minimal transcription terminator from phage lambda
                     (Scholtissek and Grosse, 1987)"
     CDS             complement(2973..3764)
                     /label="NeoR/KanR"
                     /note="aminoglycoside phosphotransferase"
     rep_origin      4176..4764
                     /direction=RIGHT
                     /label="ori"
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
                     replication"
ORIGIN
        1 cgacaatatt ggctattggc cattgcatac gttgtatcta tatcataata tgtacattta
       61 tattggctca tgtccaatat gaccgccatg ttgacattga ttattgacta gttattaata
      121 gtaatcaatt acgggttcat tagttcatag cccatatatg gagttccgcg ttacataact
      181 tacggtaaat ggcccgcctg gctgaccgcc caacgacccc cgcccattga cgtcaataat
      241 gacgtatgtt cccatagtaa cgccaatagg gactttccat tgacgtcaat gggtggagta
      301 tttacggtaa actgcccact tggcagtaca tcaagtgtat catatgccaa gtccgccccc
      361 tattgacgtc aatgacggta aatggcccgc ctggcattat gcccagtaca tgaccttacg
      421 ggactttcct acttggcagt acatctacgg tattagtcat cggctattac catggtgatg
      481 cggttttggc agtacaccaa tgggcgtgga tagcggtttg actcacgggg atttccaagt
      541 ctccacccca ttgacgtcaa tgggagtttg ttttggcacc aaaatcaacg ggactttcca
      601 aaatgtcgta ataaccccgc cccgttgacg caaatgggcg gtaggcgtgt acggtgggag
      661 gtctatataa gcagagctcg tttagtgaac cgtcagatcg cctggagacg ccatccacgc
      721 tgttttgacc tccatagaag acaccgggac cgatccagcc tccgcggccg ggaacggtgc
      781 attggaacgc ggattccccg tgccaagagt gacgtaagta ccgcctatag actctatagg
      841 cacacccctt tggctcttat gcatgctata ctgtttttgg cttggggcct atacaccccc
      901 gcttccttat gctataggtg atggtatagc ttagcctata ggtgtgggtt attgaccatt
      961 attgaccact cccctattgg tgacgatact ttccattact aatccataac atggctcttt
     1021 gccacaacta tctctattgg ctatatgcca atactctgtc cttcagagac tgacacggac
     1081 tctgtatttt tacaggatgg ggtcccattt attatttaca aattcacata tacaacaacg
     1141 ccgtcccccg tgcccgcagt ttttattaaa catagcgtgg gatctccacg cgaatctcgg
     1201 gtaccgtgtt ccggacatgg gytcttctcc ggtagcggcg gagcttccac atccgagccc
     1261 tggtcccatg cctccagcgg ctcatggtcg ctcggcagct ccttgctcct aacagtggag
     1321 gccagactta ggcacagcac aatgcccacc accaccagtg tgccgcacaa ggccgtggcg
     1381 gtagggtatg tgtctgaaaa tgagctcgga gattgggctc gcaccgctga cgcagatgga
     1441 agacttaagg cagcggcaga agaagatgca ggcagctgag ttgttgtatt ctgataagag
     1501 tcagaggtaa ctcccgttgc ggtgctgtta acggtggagg gcagtgtagt ctgagcagta
     1561 ctcgttgctg ccgcgcgcgc caccagacat aatagctgac agactaacag actgttcctt
     1621 tccatgggtc ttttctgcag tcaccatcga tgcttgcaat catggatgca atgaagagag
     1681 ggctctgctg tgtgctgctg ctgtgtggag aattcttcgt ttctgctgct gtgtggagaa
     1741 ttcttcgttt cggctagcat ggaccagcac acacttgatg tggaggatac cgcggatgcc
     1801 agacatccag caggtacttc gtgcccctcg gatgcggcgc tcctcagaga taccgggctc
     1861 ctcgcggacg ctgcgctcct ctcagatact gtgcgcccca caaatgccgc gctccccacg
     1921 gatgctgcct accctgcggt taatgttcgg gatcgcgagg ccgcgtggcc gcctgcactg
     1981 aacttctgtt cccgccaccc aaagctctat ggcctagtcg ctttggtttt gctgcttctg
     2041 atcgccgcct gtgttcctat cttcacccgc accgagcctc ggccagcgct cacaatcacc
     2101 acctcgccca acctgggtac ccgagagaat aatgcagacc aggtcacccc tgtttcccac
     2161 attggctgcc ccaacactac acaacagggc tctcctgtgt tcgccaagct actggctaaa
     2221 aaccaagcat cgttgtgcaa tacaactctg aactggcaca gccaagatgg agctgggagc
     2281 tcatacctat ctcaaggtct gaggtacgaa gaagacaaaa aggagttggt ggtagacagt
     2341 cccgggctct actacgtatt tttggaactg aagctcagtc caacattcac aaacacaggc
     2401 cacaaggtgc agggctgggt ctctcttgtt ttgcaagcaa agcctcaggt agatgacttt
     2461 gacaacttgg ccctgacagt ggaactgttc ccttgctcca tggagaacaa gttagtggac
     2521 cgttcctgga gtcaactgtt gctcctgaag gctggccacc gcctcagtgt gggtctgagg
     2581 gcttatctgc atggagccca ggatgcatac agagactggg agctgtctta tcccaacacc
     2641 accagctttg gactctttct tgtgaaaccc gacaacccat gggaatgacc taggctgtgc
     2701 cttctagttg ccagccatct gttgtttgcc cctcccccgt gccttccttg accctggaag
     2761 gtgccactcc cactgtcctt tcctaataaa atgaggaaat tgcatcgcat tgtctgagta
     2821 ggtgtcattc tattctgggg ggtggggtgg ggcaggacag caagggggag gattgggaag
     2881 acaatagcag gcatgctggg gatgcggtgg gctctatata aaaaacgccc ggcggcaacc
     2941 gagcgttctg aacgctagag tcgatcgatt cagaagaact cgtcaagaag gcgatagaag
     3001 gcgatgcgct gcgaatcggg agcggcgata ccgtaaagca cgaggaagcg gtcagcccat
     3061 tcgccgccaa gctcttcagc aatatcacgg gtagccaacg ctatgtcctg atagcggtct
     3121 gccacaccca gccggccaca gtcgatgaat ccagaaaagc ggccattttc caccatgata
     3181 ttcggcaagc aggcatcgcc atgggtcacg acgagatcct cgccgtcggg catgctcgcc
     3241 ttgagcctgg cgaacagttc ggctggcgcg agcccctgat gctcttcgtc cagatcatcc
     3301 tgatcgacaa gaccggcttc catccgagta cgtgctcgct cgatgcgatg tttcgcttgg
     3361 tggtcgaatg ggcaggtagc cggatcaagc gtatgcagcc gccgcattgc atcagccatg
     3421 atggatactt tctcggcagg agcaaggtga gatgacagga gatcctgccc cggcacttcg
     3481 cccaatagca gccagtccct tcccgcttca gtgacaacgt cgagcacagc tgcgcaagga
     3541 acgcccgtcg tggccagcca cgatagccgc gctgcctcgt cttgcagttc attcagggca
     3601 ccggacaggt cggtcttgac aaaaagaacc gggcgcccct gcgctgacag ccggaacacg
     3661 gcggcatcag agcagccgat tgtctgttgt gcccagtcat agccgaatag cctctccacc
     3721 caagcggccg gagaacctgc gtgcaatcca tcttgttcaa tcatgcgaaa cgatcctcat
     3781 cctgtctctt gatcagatct tgatcccctg cgccatcaga tccttggcgg caagaaagcc
     3841 atccagttta ctttgcaggg cttcccaacc ttaccagagg gcgccccagc tggcaattcc
     3901 ggttcgcttg ctgtccataa aaccgcccag tctagctatc gccatgtaag cccactgcaa
     3961 gctacctgct ttctctttgc gcttgcgttt tcccttgtcc agatagccca gtagctgaca
     4021 ttcatccggg gtcagcaccg tttctgcgga ctggctttct acgtgaaaag gatctaggtg
     4081 aagatccttt ttgataatct catgaccaaa atcccttaac gtgagttttc gttccactga
     4141 gcgtcagacc ccgtagaaaa gatcaaagga tcttcttgag atcctttttt tctgcgcgta
     4201 atctgctgct tgcaaacaaa aaaaccaccg ctaccagcgg tggtttgttt gccggatcaa
     4261 gagctaccaa ctctttttcc gaaggtaact ggcttcagca gagcgcagat accaaatact
     4321 gttcttctag tgtagccgta gttaggccac cacttcaaga actctgtagc accgcctaca
     4381 tacctcgctc tgctaatcct gttaccagtg gctgctgcca gtggcgataa gtcgtgtctt
     4441 accgggttgg actcaagacg atagttaccg gataaggcgc agcggtcggg ctgaacgggg
     4501 ggttcgtgca cacagcccag cttggagcga acgacctaca ccgaactgag atacctacag
     4561 cgtgagctat gagaaagcgc cacgcttccc gaagggagaa aggcggacag gtatccggta
     4621 agcggcaggg tcggaacagg agagcgcacg agggagcttc cagggggaaa cgcctggtat
     4681 ctttatagtc ctgtcgggtt tcgccacctc tgacttgagc gtcgattttt gtgatgctcg
     4741 tcaggggggc ggagcctatg gaaaaacgcc agcaacgcgg ccgctttacg gttcctggcc
     4801 ttttgctggc cttttgctca catgttgt
//
