LOCUS       V016398                 6575 bp    DNA     circular SYN 13-MAY-2019
DEFINITION  Exported.
ACCESSION   V016398
VERSION     V016398
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
            .
REFERENCE   1  (bases 1 to 6575)
  AUTHORS   Suman J.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-OCT-2018) Dpt. of Biochemistry and Microbiology,
            University of Chemistry and Technology Prague, Technicka 3, Prague,
            Czech Republic 16628, Czech Republic
REFERENCE   2  (bases 1 to 6575)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     ##Assembly-Data-START##
            Sequencing Technology :: Sanger dideoxy sequencing
            ##Assembly-Data-END##
            SGRef: number: 1; type: "Journal Article"; journalName: "Submitted
            (24-OCT-2018) Dpt. of Biochemistry and Microbiology, University of
            Chemistry and Technology Prague, Technicka 3, Prague, Czech Republic
            16628, Czech Republic"
FEATURES             Location/Qualifiers
     source          1..6575
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     regulatory      650..747
                     /label="23E5 promoter region from C. jejuni"
                     /note="23E5 promoter region from C. jejuni"
                     /regulatory_class="promoter"
     CDS             751..1068
                     /label="miniSOG"
                     /note="mini singlet oxygen generator, a fluorescent
                     flavoprotein suitable for correlated light and electron
                     microscopy (Shu et al., 2011)"
     CDS             complement(1439..1807)
                     /label="traJ"
                     /note="oriT-recognizing protein"
     oriT            complement(1840..1949)
                     /direction=LEFT
                     /label="oriT"
                     /note="incP origin of transfer"
     CDS             2054..2410
                     /codon_start=1
                     /transl_table=11
                     /product="AmpR"
                     /label="AmpR"
                     /note="class A beta-lactamase; ampicilin resistance
                     protein"
                     /protein_id="QCO69682.1"
                     /translation="MTPKQGYAAEKIRRAMATTLRKLLTGELLTLASRQQLIDWMEADK
                     VAGPLLRSALPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERN
                     RQIAEIGASLIKHW"
     rep_origin      2581..3169
                     /direction=RIGHT
                     /label="ori"
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
                     replication"
     CDS             3618..4238
                     /note="Chloramphenicol acetyltransferase from Campylobacter
                     coli. Accession#: P22782"
                     /label="Chloramphenicol acetyltransferase"
     CDS             complement(5004..6059)
                     /codon_start=1
                     /transl_table=11
                     /product="RepB family plasmid replication initiator
                     protein"
                     /label="RepB family plasmid replication initiator protein"
                     /protein_id="QCO69684.1"
                     /translation="MQMSEIVKYHNDFNKIQLPSFTEQEQNLLFLIFARIKEKGIDNVI
                     NLYANDFKINEKLSNSREYLTENINSLKYKFFKANFKQIIETKTEVIHKYVNLFETMEI
                     HYVKRNPDDGYDESTLFNRITLKINPDFAYLVNQLTTNFTAFELEEFIALSGKYAKTLY
                     RLLKQFRTTGKAYFEWDEFCKVMDIPQDYRQSEVDKWILKPAIKELSKERNLFDQIRVP
                     FKNLAYEKEKTAGRGRGGKVSGISFTFKPENIEMQKLENESQKIMSDEQKYLKILNNMK
                     LNQARFNYNDKLWQFNDFDFNEFKIIAVELAVELVRDEYENLNFGNHMHFNAKNQEQFF
                     KMIDTFRNGIR"
ORIGIN
        1 cagacagccc ccgcaccccc ttttcgcaaa tcatagcacc ttaacaggtg cgatttgcta
       61 aagcaccaaa aaagaaaact atctgctaaa cccaaaccct ctatcgtttc tttttgcttg
      121 ttcaaaagaa ttttcaaaag ctctttgata tttaagctct ttttctattc tttcggcttt
      181 taatggaaat atttctttta actgctccac gcttaaattt ttttcacacc atttgcctat
      241 ttcttttagt cgagtttcta atttaacatt ttttgttttg tgtgcctcaa acaaagtttt
      301 taaattatct cgttcatctt tgactttatg atattcaaat tttaaatatt caagctcttt
      361 gttagcttct ctcctgcttt catctgtgct aattaaaaaa gcattctttg tgatctcaca
      421 tgttctttcc tgcgttatcc cctgattctg tggataaccg tattaccgcc tttgagtgag
      481 ctgataccgc tcgccgcagc cgaacgaccg agcgcagcga gtcagtgagc gaggaagcgg
      541 aagagcgccc aatacgcaaa ccgcctctcc ccgcgcgttg gccgattcat taatgcagct
      601 ggcacgacag gtttcccgac tggaaagcgg gcagtgagcg caacgcaatc taacttttgc
      661 ttaattttag taaattttta atttatcaag ctttgataag tttatttgga tacaattgtg
      721 gttcatttta aatgaaagga acattttatg atagagaaga atttcgtcat cactgatcct
      781 aggcttcccg ataatcccat tatctttgca tcagacggct ttcttgaatt gacagagtat
      841 tcgcgcgagg aaatattggg gagaaatgcc cggtttcttc aggggccaga gacagatcaa
      901 gcgactgtcc agaagataag agacgcaatt agagatcaga gggagactac tgtgcagttg
      961 ataaactaca ctaaaagcgg aaagaaattc tggaacttac tccacctgca acctgtgcgt
     1021 gatcagaagg gagagcttca atacttcatc ggtgtgcagc tcgatggaag tgatcatgta
     1081 taaggtgatg gttcacgtag tgggccatcg ccctgataga cggtttttcg ccctttgacg
     1141 ttggagtcca cgttctttaa tagtggactc ttgttccaaa ctggaacaac actcaaccct
     1201 atctcggtct attcttttga tttataaggg attttgccga tttcggccta ttggttaaaa
     1261 aatgagctga tttaacaaaa atttaacgcg aattttaaca aaatggggat cggtcttgcc
     1321 ttgctcgtcg gtgatgtact tcaccagctc cgcgaagtcg ctcttcttga tggagcgcat
     1381 ggggacgtgc ttggcaatca cgcgcacccc ccggccgttt tagcggctaa aaaagtcatg
     1441 gctctgccct cgggcggacc acgcccatca tgaccttgcc aagctcgtcc tgcttctctt
     1501 cgatcttcgc cagcagggcg aggatcgtgg catcaccgaa ccgcgccgtg cgcgggtcgt
     1561 cggtgagcca gagtttcagc aggccgccca ggcggcccag gtcgccattg atgcgggcca
     1621 gctcgcggac gtgctcatag tccacgacgc ccgtgatttt gtagccctgg ccgacggcca
     1681 gcaggtaggc cgacaggctc atgccggccg ccgccgcctt ttcctcaatc gctcttcgtt
     1741 cgtctggaag gcagtacacc ttgataggtg ggctgccctt cctggttggc ttggtttcat
     1801 cagccatccg cttgccctca tctgttacgc cggcggtagc cggccagcct cgcagagcag
     1861 gattcccgtt gagcaccgcc aggtgcgaat aagggacagt gaagaaggaa cacccgctcg
     1921 cgggtgggcc tacttcacct atcctgcccg gctgacgccg ttggatacac caaggaaagt
     1981 ctctacacga accctttggc aaaatcctgt atatcgtgcg aaaaaggatg gatataccga
     2041 aaaaatcgct ataatgaccc cgaagcaggg ttatgcagcg gaaaagatcc gtcgagcaat
     2101 ggcaacaacg ttgcgcaaac tattaactgg cgaactactt actctagctt cccggcaaca
     2161 attaatagac tggatggagg cggataaagt tgcaggacca cttctgcgct cggcccttcc
     2221 ggctggctgg tttattgctg ataaatctgg agccggtgag cgtgggtctc gcggtatcat
     2281 tgcagcactg gggccagatg gtaagccctc ccgtatcgta gttatctaca cgacggggag
     2341 tcaggcaact atggatgaac gaaatagaca gatcgctgag ataggtgcct cactgattaa
     2401 gcattggtaa ctgtcagacc aagtttactc atatatactt tagattgatt taaaacttca
     2461 tttttaattt aaaaggatct aggtgaagat cctttttgat aatctcatga ccaaaatccc
     2521 ttaacgtgag ttttcgttcc actgagcgtc agaccccgta gaaaagatca aaggatcttc
     2581 ttgagatcct ttttttctgc gcgtaatctg ctgcttgcaa acaaaaaaac caccgctacc
     2641 agcggtggtt tgtttgccgg atcaagagct accaactctt tttccgaagg taactggctt
     2701 cagcagagcg cagataccaa atactgtcct tctagtgtag ccgtagttag gccaccactt
     2761 caagaactct gtagcaccgc ctacatacct cgctctgcta atcctgttac cagtggctgc
     2821 tgccagtggc gataagtcgt gtcttaccgg gttggactca agacgatagt taccggataa
     2881 ggcgcagcgg tcgggctgaa cggggggttc gtgcacacag cccagcttgg agcgaacgac
     2941 ctacaccgaa ctgagatacc tacagcgtga gctatgagaa agcgccacgc ttcccgaagg
     3001 gagaaaggcg gacaggtatc cggtaagcgg cagggtcgga acaggagagc gcacgaggga
     3061 gcttccaggg ggaaacgcct ggtatcttta tagtcctgtc gggtttcgcc acctctgact
     3121 tgagcgtcga tttttgtgat gctcgtcagg ggggcggagc ctatggaaaa acgccagcaa
     3181 cgcggcccga aattctcatg tttgacagct tatcatcgat aaacccagcg aaccatttga
     3241 ggtgataggt aagattatac cgaggtatga aaacgagaat tggaccttta cagaattact
     3301 ctatgaagcg ccatatttaa aaagctacca agacgaagag gatgaagagg atgaggaggc
     3361 agattgcctt gaatatattg acaatactga taagataata tataatatat ctttactacc
     3421 aagacgataa atgcgtcgga aaagttaaac tgcgaaaaaa ttggaaccgg tacgcttata
     3481 tagaagatct gctcggcggt gttcctttcc aagttaattg cgtgatatag attgaaaagt
     3541 ggatagattt atgatatagt ggatagattt atgatataat gagttatcaa caaatcggaa
     3601 tttacggagg ataaatgatg caattcacaa agattgatat aaataattgg acacgaaaag
     3661 agtatttcga ccactatttt ggcaatacgc cctgcacata tagtatgacg gtaaaactcg
     3721 atatttctaa gttgaaaaag gatggaaaaa agttataccc aactctttta tatggagtta
     3781 caacgatcat caatcgacat gaagagttca ggaccgcatt agatgaaaac ggacaggtag
     3841 gcgttttttc agaaatgctg ccttgctaca cagtttttca taaggaaact gaaacctttt
     3901 cgagtatttg gactgagttt acagcagact atactgagtt tcttcagaac tatcaaaagg
     3961 atatagacgc ttttggtgaa cgaatgggaa tgtccgcaaa gcctaatcct ccggaaaaca
     4021 ctttccctgt ttctatgata ccgtggacaa gctttgaagg ctttaactta aatctaaaaa
     4081 aaggatatga ctatctactg ccgatattta cgtttgggaa gtattatgag gagggcggaa
     4141 aatactatat tcccttatcg attcaagtgc atcatgccgt ttgtgacggc tttcatgttt
     4201 gccgtttttt ggatgaatta caagacttgc tgaataaata aaatcccagt ttgtcgcact
     4261 gataaaaacc ctttaggaac taaagggcgc agatcgggga agaacagtat gtcgagctat
     4321 tttttgactt actgggggat caagcctgat tgggagaaaa taaaatatta tattttactg
     4381 gatgaattgt tttagtacct agatttagat gtctaaaaag cttttttata aagcctttct
     4441 atgttcttac gcacttcatc gctagtgcaa gagtattcat ttaaatgtgt aagttcttta
     4501 gtgattgtct tagcattatc gttggttcta tcattgtggt ttaatgcagg ttgtttttta
     4561 ggattgcaat gagcggtggc aacttttgac attttgcatt catactcccc attctttctt
     4621 taatgcaaca atacattcat tagctgttgc ttcgcctttt ataattttaa cattatcaat
     4681 aatatctttt tcattcaaaa acatatcttc tattgcaaaa ttacctatat ttgatcttat
     4741 ggcttcgtat tgctctttag tcaaatcatc tttatatttt ttaagtatcg caaggctttc
     4801 ttctaagcta tgcacttcgg gatattgttt ttgcattttt aaatttttcc ttttttggtg
     4861 ttttaataaa aatcaaaaaa tcattcactg attttagttg atttttatta gtggggttaa
     4921 ggggttgcac cccttacaag ctcgacactt ggcacaagtg gcggatagct tgtatccttc
     4981 aaagatataa ttatagcaaa tttttaccta atcccattcc taaaagtatc aatcatttta
     5041 aaaaactgct cttgattttt agcattaaag tgcatatgat ttccaaagtt taaattctcg
     5101 tattcatctc ttacaagctc tactgcaagc tctactgcaa taattttaaa ttcattaaaa
     5161 tcaaaatcgt taaactgcca aagcttgtca ttataattaa atctagcttg attaagtttc
     5221 atattgttta aaatctttaa atatttttgc tcatcgctca ttattttttg actttcattt
     5281 tctagctttt gcatttcgat attttcaggt ttaaaagtaa agctaatgcc tgaaactttg
     5341 ccgccacgcc cacgccctgc ggttttttct ttttcataag caagattttt aaaaggcact
     5401 ctaatttggt caaaaagatt gcgttcttta gaaagttctt tgatagcagg ttttaaaatc
     5461 catttatcga cttcgctttg tctataatct tgtggtatat ccataacttt acaaaactcg
     5521 tcccactcaa aataagcttt gccagtggtt ctaaattgct ttaaaaggcg gtaaagtgtt
     5581 ttagcatatt taccgctaag ggctataaat tcttcaagct caaaagctgt aaaattagta
     5641 gttagttgat tgactaaata agcaaaatca gggtttattt ttagggttat tctgttaaat
     5701 aaagtgcttt catcatatcc atcatcagga tttcttttaa catagtgtat ttccattgtt
     5761 tcaaacaaat ttacatattt gtgtataact tctgtctttg tttctatgat ttgtttaaaa
     5821 tttgccttga aaaatttata ttttaagcta ttgatatttt ctgttaaata ttctcttgag
     5881 ttagaaagtt tttcattaat tttaaaatca ttagcataga gattaattac attgtctatg
     5941 cctttttctt taattcttgc aaagattaaa aacaaaaggt tttgttcttg ctctgtaaag
     6001 ctaggcaact gaattttatt aaaatcgttg tgatatttaa ctatttcgct catttgcata
     6061 tccaaagtaa gacaatcaag gcaaggggct tttccgcccc tgcggttttg ttagaaaaat
     6121 acttcaactt taagcttaaa aaacctaaaa tttaggtata atctcacaaa acgcttacta
     6181 tgtcgtttca tagttagcct tgattgataa atttccgcca agagtgaaaa ctcaaggcgg
     6241 attttttatt gtagcatatt aaaaatagat tgtcaatagt ttctaccctt aatacctacc
     6301 atttctaccc ttaataccta ccatttctac ccttaatacc taccatttct acccttaata
     6361 cctaccattt ctacccttaa tacctaccat ttctaccctt aatatttcgc tagcccccat
     6421 catatagggc ttagaaagcc ccctaaaaat atataaaaat atataaaaaa taaaaaaatt
     6481 cgcgtacgcg tacgcgcgcg agagaaaaaa caaaaaaagt tgagtaattt tttttctagg
     6541 gcggtgggct ttgtccgtct tccgtgctac gcact
//
