LOCUS       62056_19085        4783 bp DNA     circular SYN 20-JAN-2020
DEFINITION  Cloning vector pRTL, complete sequence.
ACCESSION   MN728544
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 4783)
  AUTHORS   Wang L.
  TITLE     Direct Submission
  JOURNAL   Submitted (22-NOV-2019) State Key Laboratory of Plant Genomics, 
            Institute of Genetics and Developmental Biology, CAS, West Beichen 
            Road, Bei Jing 100101, China
REFERENCE   2  (bases 1 to 4783)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Submitted 
            (22-NOV-2019) State Key Laboratory of Plant Genomics, Institute of 
            Genetics and Developmental Biology, CAS, West Beichen Road, Bei Jing
            100101, China"
FEATURES             Location/Qualifiers
     source          1..4783
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     rep_origin      856..1444
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     protein_bind    1732..1753
                     /label=CAP binding site
                     /note="CAP binding activates transcription in the presence
                     of cAMP."
     promoter        1768..1798
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    1806..1822
                     /label=lac operator
                     /note="The lac repressor binds to the lac operator to
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     1830..1846
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     promoter        2370..2715
                     /label=CaMV 35S promoter
                     /note="strong constitutive promoter from cauliflower mosaic
                     virus"
     CDS             2799..3731
                     /codon_start=1
                     /label=Rluc
                     /note="luciferase from the anthozoan coelenterate Renilla 
                     reniformis (sea pansy)"
                     /translation="MTSKVYDPEQRKRMITGPQWWARCKQMNVLDSFINYYDSEKHAEN
                     AVIFLHGNAASSYLWRHVVPHIEPVARCIIPDLIGMGKSGKSGNGSYRLLDHYKYLTAW
                     FELLNLPKKIIFVGHDWGACLAFHYSYEHQDKIKAIVHAESVVDVIESWDEWPDIEEDI
                     ALIKSEEGEKMVLENNFFVETMLPSKIMRKLEPEEFAAYLEPFKEKGEVRRPTLSWPRE
                     IPLVKGGKPDVVQIVRNYNAYLRASDDLPKMFIESDPGFFSNAIVEGAKKFPNTEFVKV
                     KGLHFSQEDAPDEMGKYIKSFVERVLKNEQ"
     terminator      3752..4004
                     /label=NOS terminator
                     /note="nopaline synthase terminator and poly(A) signal"
     primer_bind     complement(4013..4029)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     promoter        4503..4607
                     /label=AmpR promoter
     CDS             join(4608..4783,1..682)
                     /codon_start=1
                     /label=AmpR
                     /note="beta-lactamase"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
ORIGIN
        1 ccccgaagaa cgttttccaa tgatgagcac ttttaaagtt ctgctatgtg gcgcggtatt
       61 atcccgtatt gacgccgggc aagagcaact cggtcgccgc atacactatt ctcagaatga
      121 cttggttgag tactcaccag tcacagaaaa gcatcttacg gatggcatga cagtaagaga
      181 attatgcagt gctgccataa ccatgagtga taacactgcg gccaacttac ttctgacaac
      241 gatcggagga ccgaaggagc taaccgcttt tttgcacaac atgggggatc atgtaactcg
      301 ccttgatcgt tgggaaccgg agctgaatga agccatacca aacgacgagc gtgacaccac
      361 gatgcctgta gcaatggcaa caacgttgcg caaactatta actggcgaac tacttactct
      421 agcttcccgg caacaattaa tagactggat ggaggcggat aaagttgcag gaccacttct
      481 gcgctcggcc cttccggctg gctggtttat tgctgataaa tctggagccg gtgagcgtgg
      541 gtctcgcggt atcattgcag cactggggcc agatggtaag ccctcccgta tcgtagttat
      601 ctacacgacg gggagtcagg caactatgga tgaacgaaat agacagatcg ctgagatagg
      661 tgcctcactg attaagcatt ggtaactgtc agaccaagtt tactcatata tactttagat
      721 tgatttaaaa cttcattttt aatttaaaag gatctaggtg aagatccttt ttgataatct
      781 catgaccaaa atcccttaac gtgagttttc gttccactga gcgtcagacc ccgtagaaaa
      841 gatcaaagga tcttcttgag atcctttttt tctgcgcgta atctgctgct tgcaaacaaa
      901 aaaaccaccg ctaccagcgg tggtttgttt gccggatcaa gagctaccaa ctctttttcc
      961 gaaggtaact ggcttcagca gagcgcagat accaaatact gttcttctag tgtagccgta
     1021 gttaggccac cacttcaaga actctgtagc accgcctaca tacctcgctc tgctaatcct
     1081 gttaccagtg gctgctgcca gtggcgataa gtcgtgtctt accgggttgg actcaagacg
     1141 atagttaccg gataaggcgc agcggtcggg ctgaacgggg ggttcgtgca cacagcccag
     1201 cttggagcga acgacctaca ccgaactgag atacctacag cgtgagctat gagaaagcgc
     1261 cacgcttccc gaagggagaa aggcggacag gtatccggta agcggcaggg tcggaacagg
     1321 agagcgcacg agggagcttc cagggggaaa cgcctggtat ctttatagtc ctgtcgggtt
     1381 tcgccacctc tgacttgagc gtcgattttt gtgatgctcg tcaggggggc ggagcctatg
     1441 gaaaaacgcc agcaacgcgg cctttttacg gttcctggcc ttttgctggc cttttgctca
     1501 catgttcttt cctgcgttat cccctgattc tgtggataac cgtattaccg cctttgagtg
     1561 agctgatacc gctcgccgca gccgaacgac cgagcgcagc gagtcagtga gcgaggaagc
     1621 ggaagagcgc ccaatacgca aaccgcctct ccccgcgcgt tggccgattc attaatgcag
     1681 ctggcacgac aggtttcccg actggaaagc gggcagtgag cgcaacgcaa ttaatgtgag
     1741 ttagctcact cattaggcac cccaggcttt acactttatg cttccggctc gtatgttgtg
     1801 tggaattgtg agcggataac aatttcacac aggaaacagc tatgaccatg attacgccaa
     1861 gcttgcatgc ctgcaggtcc ccagattagc cttttcaatt tcagaaagaa tgctaaccca
     1921 cagatggtta gagaggctta cgcagcaggt ctcatcaaga cgatctaccc gagcaataat
     1981 ctccaggaaa tcaaatacct tcccaagaag gttaaagatg cagtcaaaag attcaggact
     2041 aactgcatca agaacacaga gaaagatata tttctcaaga tcagaagtac tattccagta
     2101 tggacgattc aaggcttgct tcacaaacca aggcaagtaa tagagattgg agtctctaaa
     2161 aaggtagttc ccactgaatc aaaggccatg gagtcaaaga ttcaaataga ggacctaaca
     2221 gaactcgccg taaagactgg cgaacagttc atacagagtc tcttacgact caatgacaag
     2281 aagaaaatct tcgtcaacat ggtggagcac gacacacttg tctactccaa aaatatcaaa
     2341 gatacagtct cagaagacca aagggcaatt gagacttttc aacaaagggt aatatccgga
     2401 aacctcctcg gattccattg cccagctatc tgtcacttta ttgtgaagat agtggaaaag
     2461 gaaggtggct cctacaaatg ccatcattgc gataaaggaa aggccatcgt tgaagatgcc
     2521 tctgccgaca gtggtcccaa agatggaccc ccacccacga ggagcatcgt ggaaaaagaa
     2581 gacgttccaa ccacgtcttc aaagcaagtg gattgatgtg atatctccac tgacgtaagg
     2641 gatgacgcac aatcccacta tccttcgcaa gacccttcct ctatataagg aagttcattt
     2701 catttggaga gaacacgggg gactctagag gatccacaat taccaacaac aacaaacaac
     2761 aaacaacatt acaattacag atccctcgac tagccaccat gacttcgaaa gtttatgatc
     2821 cagaacaaag gaaacggatg ataactggtc cgcagtggtg ggccagatgt aaacaaatga
     2881 atgttcttga ttcatttatt aattattatg attcagaaaa acatgcagaa aatgctgtta
     2941 tttttttaca tggtaacgcg gcctcttctt atttatggcg acatgttgtg ccacatattg
     3001 agccagtagc gcggtgtatt ataccagacc ttattggtat gggcaaatca ggcaaatctg
     3061 gtaatggttc ttataggtta cttgatcatt acaaatatct tactgcatgg tttgaacttc
     3121 ttaatttacc aaagaagatc atttttgtcg gccatgattg gggtgcttgt ttggcatttc
     3181 attatagcta tgagcatcaa gataagatca aagcaatagt tcacgctgaa agtgtagtag
     3241 atgtgattga atcatgggat gaatggcctg atattgaaga agatattgcg ttgatcaaat
     3301 ctgaagaagg agaaaaaatg gttttggaga ataacttctt cgtggaaacc atgttgccat
     3361 caaaaatcat gagaaagtta gaaccagaag aatttgcagc atatcttgaa ccattcaaag
     3421 agaaaggtga agttcgtcgt ccaacattat catggcctcg tgaaatcccg ttagtaaaag
     3481 gtggtaaacc tgacgttgta caaattgtta ggaattataa tgcttatcta cgtgcaagtg
     3541 atgatttacc aaaaatgttt attgaatcgg acccaggatt cttttccaat gctattgttg
     3601 aaggtgccaa gaagtttcct aatactgaat ttgtcaaagt aaaaggtctt catttttcgc
     3661 aagaagatgc acctgatgaa atgggaaaat atatcaaatc gttcgttgag cgagttctca
     3721 aaaatgaaca ataattctag cgaatttccc cgatcgttca aacatttggc aataaagttt
     3781 cttaagattg aatcctgttg ccggtcttgc gatgattatc atataatttc tgttgaatta
     3841 cgttaagcat gtaataatta acatgtaatg catgacgtta tttatgagat gggtttttat
     3901 gattagagtc ccgcaattat acatttaata cgcgatagaa aacaaaatat agcgcgcaaa
     3961 ctaggataaa ttatcgcgcg cggtgtcatc tatgttacta gatcgggaat tcactggccg
     4021 tcgttttaca acgtcgtgac tgggaaaacc ctggcgttac ccaacttaat cgccttgcag
     4081 cacatccccc tttcgccagc tggcgtaata gcgaagaggc ccgcaccgat cgcccttccc
     4141 aacagttgcg cagcctgaat ggcgaatggc gcctgatgcg gtattttctc cttacgcatc
     4201 tgtgcggtat ttcacaccgc atatggtgca ctctcagtac aatctgctct gatgccgcat
     4261 agttaagcca gccccgacac ccgccaacac ccgctgacgc gccctgacgg gcttgtctgc
     4321 tcccggcatc cgcttacaga caagctgtga ccgtctccgg gagctgcatg tgtcagaggt
     4381 tttcaccgtc atcaccgaaa cgcgcgagac gaaagggcct cgtgatacgc ctatttttat
     4441 aggttaatgt catgataata atggtttctt agacgtcagg tggcactttt cggggaaatg
     4501 tgcgcggaac ccctatttgt ttatttttct aaatacattc aaatatgtat ccgctcatga
     4561 gacaataacc ctgataaatg cttcaataat attgaaaaag gaagagtatg agtattcaac
     4621 atttccgtgt cgcccttatt cccttttttg cggcattttg ccttcctgtt tttgctcacc
     4681 cagaaacgct ggtgaaagta aaagatgctg aagatcagtt gggtgcacga gtgggttaca
     4741 tcgaactgga tctcaacagc ggtaagatcc ttgagagttt tcg
//
