LOCUS       40924_38308        8071 bp DNA     circular SYN 18-DEC-2018
DEFINITION  Expression vector pRT108, complete sequence.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 8071)
  AUTHORS   Thomas R, Chege G, Shephard E, Bourn W, Maclean J, Jaffray A, Tanzer
            F, Steyn L, Berkower C, Jacobs W Jr., Rybicki E, Williamson A-L.
  TITLE     Construction and stability analysis of different recombinant BCG 
            expressing HIV subtype C Pr55 Gag that induce an immune response in 
            baboons which can be boosted by HIV-1 subtype C Pr55 Gag virus-like 
            particles
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 8071)
  AUTHORS   Thomas R, Chege G, Shephard E, Bourn W, Maclean J, Jaffray A, Tanzer
            F, Steyn L, Berkower C, Jacobs W Jr., Rybicki E, Williamson A-L.
  TITLE     Direct Submission
  JOURNAL   Submitted (20-JAN-2005) Medical Virology, University of Cape Town, 
            Anzio Road, Cape Town, Western Cape 7925, South Africa
REFERENCE   3  (bases 1 to 8071)
  TITLE     Direct Submission
REFERENCE   4  (bases 1 to 8071)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: 
            "Unpublished"
COMMENT     SGRef: number: 2; type: "Journal Article"; journalName: "Submitted 
            (20-JAN-2005) Medical Virology, University of Cape Town, Anzio Road,
            Cape Town, Western Cape 7925, South Africa"
COMMENT     SGRef: number: 3; type: "Journal Article"
FEATURES             Location/Qualifiers
     source          1..8071
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     CDS             1877..2689
                     /codon_start=1
                     /label=KanR
                     /note="aminoglycoside phosphotransferase"
                     /translation="MSHIQRETSCSRPRLNSNMDADLYGYKWARDNVGQSGATIYRLYG
                     KPHAPELFLKHGKGSVANDVTDEMVRLNWLTEFMPLPTIKHFIRTPDDAWLLTTAIPGK
                     TAFQVLEEYPDSGENIVDALAVFLRRLHSIPVCNCPFNSDRVFRLAQAQSRMNNGLVDA
                     SDFDDERNGWPVEQVWKEMHNLLPFSPDSVVTHGDFSLDNLIFDEGKLIGCIDVGRVGI
                     ADRYQDLAILWNCLGEFSPSLQKRLFQKYGIDNPDMNKLQFHLMLDEFF"
     rep_origin      3024..3612
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     CDS             6406..7884
                     /codon_start=1
                     /product="Gag"
                     /label=Gag
                     /note="HIV-1 Gag from subtype C isolate DU422 from South
                     Africa"
                     /protein_id="AAX11267.1"
                     /translation="MGARASILRGEKLDTWEKIRLRPGGKKHYMLKHIVWASRELERFA
                     LNPGLLETSEGCKQIIKQLQPALQTGTEELKSLYDTVATLYCVHEKIEVRDTKEALDKI
                     EEEQNKCQQKTQQAKAADGKVSQNYPIVQNLQGQMVHQAISPRTLNAWVKVIEEKAFSP
                     EVIPMFTALSEGATPQDLNTMLNTVGGHQAAMQMLKDTINEEAAEWDRLHPVHAGPIAP
                     GQMREPRGSDIAGTTSTLQEQIAWMTSNPPIPVGDIYKRWIILGLNKIVRMYSPVSILD
                     IRQGPKEPFRDYVDRFFKTLRAEQATQEVKNWMTDTLLVQNANPDCKTILRALGPGATL
                     EEMMTACQGVGGPGHKARVLAEAMSQTNSGNIMMQRSNFKGPRRIVKCFNCGKEGHIAR
                     NCRAPRKKGCWKCGKEGHQMKDCTERQANFLGKIWPSHKGRPGNFLQNRPEPTAPPAES
                     FRLEETTPAPKQEPIEREPLTSLKSLFGSDPLSQ"
     terminator      7989..8035
                     /label=rrnB T1 terminator
                     /note="transcription terminator T1 from the E. coli rrnB
                     gene"
ORIGIN
        1 gctagaggtg accacaacga cgcgcccgct ttgatcgggg acgtctgcgg ccgaccattt
       61 acgggtcttg ttgtcgttgg cggtcatggg ccgaacatac tcacccggat cggagggccg
      121 aggacaaggt cgaacgaggg gcatgacccg gtgcggggct tcttgcactc ggcataggcg
      181 agtgctaaga ataacgttgg cactcgcgac cggtgagtcg taggtcggga cggtgaggcc
      241 aggcccgtcg tcgcagcgag tggcagcgag gacaacttga gccgtccgtc gcgggcactg
      301 cgcccggcca gcgtaagtag cggggttgcc gtcacccggt gacccccggt ttcatccccg
      361 atccggagga atcacttcgc aatggccaag acaattgcgg atccagatgt gaacgagctg
      421 ctgcacttag cgccgaatgt gtggccgcgc aatactactc gcgatgaagt cggtgtggtc
      481 tgcatcgcag gaattccact gacgcagctc gcccaggagt acgggacccc gctgttcgtc
      541 atcgacgagg acgactttcg ctcgcgctgc cgagaaaccg ccgcggcctt tggaagtggg
      601 gcgaacgtgc actatgccgc caaggcgttc ctgtgcagcg aagtagcccg gtggatcagc
      661 gaagaagggc tctgtctgga cgtttgcacc ggtggggagt tggcggtcgc gctgcacgct
      721 agctttccgc ccgagcgaat taccttgcac ggcaacaaca aatcggtctc agagttgacc
      781 gctgcggtca aagccggagt cggccatatt gtcgtcgatt cgatgaccga gatcgagcgc
      841 ctcgacgcca tcgcgggcga ggccggaatc gtccaggatg tcctggtgcg tctcaccgtc
      901 ggtgtcgagg cgcacaccca cgagttcatc tccaccgcgc acgaggacca gaaattcggg
      961 ttatcggtgg ccagcggcgc ggccatggca gcggtgcggc gcgttttcgc cactgatcac
     1021 ctgcgcctgg ttgggctaca cagccacatc ggttcgcaga tcttcgacgt ggacggcttc
     1081 gaactcgccg cgcaccgtgt catcggcctg ctacgcgacg tcgtcggcga gttcggtccc
     1141 gaaaagacgg cacagatcgc gaccgtcgat ctcggtggcg gcttgggcat ctcgtatttg
     1201 ccgtccgacg acccaccgcc gatagccgag ctcgcggcca agctgggtac catcgtgagc
     1261 gacgagtcaa cggccgtggg gctgccgacg cccaagctcg ttgtggagcc cggacgcgcc
     1321 atcgccggac cgggcaccat cacgttgtat gaggtcggca ccgttaagga cgtcgatgtc
     1381 agcgccacag cgcatcgacg ttacgtcagt gtcgacggcg gcatgagcga caacatccgc
     1441 accggcctct acggcgcgca gtatgacgtc cggctggtgt ctcgagtcag cgacgccccg
     1501 ccggtaccgg cccgtctggt cggaaagcac tgcgaaagtg gcgatatcat cgtgcgggac
     1561 acctgggtgc cgacgatatt cggcccggcg atctggttgc ggttgccgcc accggcctta
     1621 ctgctattcg ctgtcgagtc gttacaacat ggtcggccgt cccgctgtgg tagcggtgca
     1681 cgcggcaacg ctcgcctggt cctgcgtcgg gagacggtcg acgatttgct gagtttggaa
     1741 gtgaggtgaa ctagtctcta gccaacaaag cgacgttgtg tctcaaaatc tctgatgtta
     1801 cattgcacaa gataaaaata tatcatcatg aacaataaaa ctgtctgctt acataaacag
     1861 taatacaagg ggtgttatga gccatattca acgggaaacg tcttgctcga ggccgcgatt
     1921 aaattccaac atggatgctg atttatatgg gtataaatgg gctcgcgata atgtcgggca
     1981 atcaggtgcg acaatctatc gcttgtatgg gaagccccat gcgccagagt tgtttctgaa
     2041 acatggcaaa ggtagcgttg ccaatgatgt tacagatgag atggtcagac taaactggct
     2101 gacggaattt atgcctcttc cgaccatcaa gcattttatc cgtactcctg atgatgcatg
     2161 gttactcacc actgcgatcc ccgggaaaac agcattccag gtattagaag aatatcctga
     2221 ttcaggtgaa aatattgttg atgcgctggc agtgttcctg cgccggttgc attcgattcc
     2281 tgtttgtaat tgtcctttta acagcgatcg cgtatttcgt ctcgctcagg cgcaatcacg
     2341 aatgaataac ggtttggttg atgcgagtga ttttgatgac gagcgtaatg gctggcctgt
     2401 tgaacaagtc tggaaagaaa tgcataatct tttgccattc tcaccggatt cagtcgtcac
     2461 tcatggtgat ttctcacttg ataaccttat ttttgacgag gggaaattaa taggttgtat
     2521 tgatgttgga cgagtcggaa tcgcagaccg ataccaggat cttgccatcc tatggaactg
     2581 cctcggtgag ttttctcctt cattacagaa acggcttttt caaaaatatg gtattgataa
     2641 tcctgatatg aataaattgc agtttcattt gatgctcgat gagtttttct aatcagaatt
     2701 ggttaattgg ttgtaacact ggcagagcat tacgctgact tgacgggacg gcggctttgt
     2761 tgaataaatc gaacttttgc tgagttgaag gatcagatca cgcatcttcc cgacaacgca
     2821 gaccgttccg tggcaaagca aaagttcaaa atcaccaact ggtccaccta caacaaagct
     2881 ctcatcaacc gtggctccct cactttctgg ctggatgatg gggcgattca ggcctggtat
     2941 gagtcagcaa caccttcttc acgaggcaga cctcactagt tccactgagc gtcagacccc
     3001 gtagaaaaga tcaaaggatc ttcttgagat cctttttttc tgcgcgtaat ctgctgcttg
     3061 caaacaaaaa aaccaccgct accagcggtg gtttgtttgc cggatcaaga gctaccaact
     3121 ctttttccga aggtaactgg cttcagcaga gcgcagatac caaatactgt ccttctagtg
     3181 tagccgtagt taggccacca cttcaagaac tctgtagcac cgcctacata cctcgctctg
     3241 ctaatcctgt taccagtggc tgctgccagt ggcgataagt cgtgtcttac cgggttggac
     3301 tcaagacgat agttaccgga taaggcgcag cggtcgggct gaacgggggg ttcgtgcaca
     3361 cagcccagct tggagcgaac gacctacacc gaactgagat acctacagcg tgagcattga
     3421 gaaagcgcca cgcttcccga agggagaaag gcggacaggt atccggtaag cggcagggtc
     3481 ggaacaggag agcgcacgag ggagcttcca gggggaaacg cctggtatct ttatagtcct
     3541 gtcgggtttc gccacctctg acttgagcgt cgatttttgt gatgctcgtc aggggggcgg
     3601 agcctatgga aaaacgccag caacgcggcc tttttacggt tcctggcctt ttgctggcct
     3661 tttgctcaca tgttctttcc tgcgttatcc cctgattctg tggataaccg tattaccgcc
     3721 tttgagtgag ctgataccgc tcgccgcagc cgaacgaccg agcgcaacgc gtgagcccac
     3781 cagctccgta agttcgggtg ctgtgtggct cgtacccgcg cattcaggcg gcagggggtc
     3841 taacgggtct aaggcggcgt gtacggccgc cacagcggct cttagcggcc cggaaacgtc
     3901 ctcgaaacga cgcatgtgtt cctcctggtt ggtacaggtg gttgggggtg ctcggctgtc
     3961 gctggtgttt catcatcagg gctcgacggg agagcggggg agtgtgcagt tgtggggtgg
     4021 cccctcagcg aaatatctga cttggagctc gtgtcggacc atacaccggt gattaatcgt
     4081 ggtttattat caagcgtgag ccacgtcgcc gacgaatttg agcagctctg gctgccgtac
     4141 tggtccctgg caagcgacga tctgctcgag gggatctacc gccaaagccg cgcgtcggcc
     4201 ctaggccgcc ggtacatcga ggcgaaccca acagcgctgg caaacctgct ggtcgtggac
     4261 gtagaccatc cagacgcagc gctccgagcg ctcagcgccc gggggtccca tccgctgccc
     4321 aacgcgatcg tgggcaatcg cgccaacggc cacgcacacg cagtgtgggc actcaacgcc
     4381 cctgttccac gcaccgaata cgcgcggcgt aagccgctcg catacatggc ggcgtgcgcc
     4441 gaaggccttc ggcgcgccgt cgatggcgac cgcagttact caggcctcat gaccaaaaac
     4501 cccggccaca tcgcctggga aacggaatgg ctccactcag atctctacac actcagccac
     4561 atcgaggccg agctcggcgc gaacatgcca ccgccgcgct ggcgtcagca gaccacgtac
     4621 aaagcggctc cgacgccgct agggcggaat tgcgcactgt tcgattccgt caggttgtgg
     4681 gcctatcttc ccgccctcat gcggatctac ctgccgaccc ggaacgtgga cggactcggc
     4741 cgcgcgatct atgccgagtg ccacgcgcga aacgccgaat ttccgtgcaa cgacgtgtgt
     4801 cccggaccgc taccggacag cgaggtccgc gccatcgcca acagcatttg gcgttggatc
     4861 acaaccaagt cgcgcatttg ggcggacggg atcgtggtct acgaggccac actcagtgcg
     4921 cgccatgcgg ccatctcgcg gaagggcgca gcagcgcgca cggcggcgag cacagttgcg
     4981 cggcgcgcaa agtccgcgtc agccatggag gcattgctat gagcgacggc tacagcgacg
     5041 gctacagcga cggctacaac tggcagccga ctgtccgcaa aaagcggcgc gtgaccgccg
     5101 ccgaaggcgc tcgaatcacc ggactatccg aacgccacgt cgtccggctc gtggcgcagg
     5161 aacgcagcga gtggttcgcc gagcaggctg cacgccgcga acgcatccgc gcctatcacg
     5221 acgacgaggg ccactcttgg ccgcaaacgg ccaaacattt cgggctgcat ctggacaccg
     5281 ttaagcgact cggctatcgg gcgaggaaag agcgtgcggc agaacaggaa gcggctcaaa
     5341 aggcccacaa cgaagccgac aatccaccgc tgttctaacg caattgggga gcgggtgtcg
     5401 cgggggttcc gtggggggtt ccgttgcaac gggtcggaca ggtaaaagtc ctggtagacg
     5461 ctagttttct ggtttgggcc atgcctgtct cgttgcgtgt ttcgttgcgt ccgttttgaa
     5521 taccagccag acgagacggg gttctacgaa tcttggtcga taccaagcca tttccgctga
     5581 atatcgtgga gctcaccgcc agaatcggtg gttgtggtga tgtacgtggc gaactccgtt
     5641 gtagtgcttg tggtggcatc cgtggcgcgg ccgcggtacc agatctttaa atctagagat
     5701 gggcgatcgg gtcctagcag acgcctgtca cgctagccaa agtcttgact gattccagaa
     5761 aagggagtca tattgtctag tgtgtcctct ataccggact acgccgaaca gctccggacg
     5821 gccgacctgc gcgtgacccg accgcgcgtc gccgtcctgg aagcagtgaa tgcgcatcca
     5881 cacgccgaca cggaaacgat tttcggtgcc gtgcgttttg cgctgcccga cgtatccggc
     5941 aagccgtgta cgacgtgctg catgccctga ccgccgcggg cttggtgcga aagatccaac
     6001 cctcgggctc cgtcgcgcgc tacgagtcca gggtcggcga caaccaccat cacatcgtct
     6061 gccggtcttg cggggttatc gccgatgtcg actgtgctgt tggcgaggca ccctgtctga
     6121 cggcctcgga ccataacggc ttcctgttgg acgaggcgga ggtcatctac tggggtctat
     6181 gtcctgattg ttcgatatcc gacacttcgc gatcacatcc gtgatcacag cccgataaca
     6241 ccaactcctg gaaggatcca tatgaagcgt ggactgacgg tcgcggtagc cggagccgcc
     6301 attctggtcg caggtctttc cggatgttca agcaacaagt cgactacagg aagcggtgag
     6361 accacgaccg cggcaggcac gacggcaagc cccggcggtg ggcccatggg tgcgagagcg
     6421 tcaatattaa gaggggaaaa attagataca tgggaaaaaa ttaggttaag gccaggggga
     6481 aagaaacatt atatgttaaa acacatagta tgggcaagca gggagctgga aagatttgca
     6541 cttaaccctg gccttttaga aacatcagaa ggatgtaaac aaataataaa acagctacaa
     6601 ccagctctcc agacaggaac agaggaactt aaatcattat acgacacagt agcaactctc
     6661 tattgtgtac atgaaaagat agaagtacga gacaccaagg aagccttaga taagatagag
     6721 gaagaacaaa acaaatgtca gcaaaaaacg cagcaggcaa aagcggctga cgggaaagtc
     6781 agtcaaaatt atcctatagt gcagaatctc caagggcaaa tggtacatca agccatatca
     6841 cctagaacct tgaatgcatg ggtaaaagta atagaggaaa aggcttttag cccagaggta
     6901 atacccatgt ttacagcatt atcagaagga gccaccccac aagatttaaa caccatgtta
     6961 aatacggtgg ggggacatca agcagccatg caaatgttaa aagataccat caatgaagag
     7021 gctgcagaat gggatagatt acatccagta catgcggggc ctattgcacc aggccagatg
     7081 agagaaccaa ggggaagtga catagcagga actactagta cccttcagga acaaatagca
     7141 tggatgacaa gtaacccacc tattccagtg ggagacatct ataaaagatg gataattctg
     7201 gggttaaata aaatagtgag aatgtatagc ccggtcagca ttttggacat aagacaaggg
     7261 ccaaaggaac cctttcgaga ctatgtagat cggttcttta aaactttaag agctgaacaa
     7321 gctacacaag aagtaaaaaa ttggatgaca gacaccttgt tagtccaaaa tgcgaaccca
     7381 gattgtaaga ccattttgag agcattggga ccaggggcta cattagaaga aatgatgaca
     7441 gcatgtcaag gggtgggagg acctggccac aaagcaagag tattggctga ggcaatgagt
     7501 caaacaaaca gtggaaacat aatgatgcag agaagcaatt ttaaaggccc tagaagaatt
     7561 gttaaatgtt ttaactgtgg caaggaaggg cacatagcca gaaattgcag agcccctagg
     7621 aaaaaaggct gttggaaatg tggaaaagaa ggacaccaaa tgaaagactg cactgagagg
     7681 caggctaatt ttttagggaa aatttggcct tcccacaagg ggaggccagg gaatttcctt
     7741 cagaacagac cagagccaac agccccacca gcagagagct tcaggctcga agagacaacc
     7801 cccgctccga aacaggagcc gatagaaagg gaacccttaa cttccctcaa atcactcttt
     7861 ggcagcgacc ccttgtctca ataaaagtag ggggccagac aagggaggct ctcttagaca
     7921 caggagcaga tgatacagta ttatcgatgt cgacgtagtt aactagcgta cgatcgactg
     7981 ccaggcatca aataaaacga aaggctcagt cgaaagactg ggcctttcgt tttatctgtt
     8041 gtttgtccgg ccatcatggc cgcggtgatc a
//
