LOCUS       40924_38288        5026 bp DNA     circular SYN 18-DEC-2018
DEFINITION  Expression vector pRSVcat, complete sequence.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 5026)
  AUTHORS   Hastings KEM., Ong J, Zhan S.
  TITLE     pRSVcat chloramphenicol acetyltransferase expression vector 
            (constructed by Gorman et al 1982)
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 5026)
  AUTHORS   Hastings KEM., Ong J, Zhan S.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-MAY-2005) Montreal Neurological Institute, McGill 
            University, 3801 University Street, Montreal, QC H3A 2B4, Canada
REFERENCE   3  (bases 1 to 5026)
  TITLE     Direct Submission
REFERENCE   4  (bases 1 to 5026)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: 
            "Unpublished"
COMMENT     SGRef: number: 2; type: "Journal Article"; journalName: "Submitted 
            (25-MAY-2005) Montreal Neurological Institute, McGill University, 
            3801 University Street, Montreal, QC H3A 2B4, Canada"
COMMENT     SGRef: number: 3; type: "Journal Article"
FEATURES             Location/Qualifiers
     source          1..5026
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        complement(7..268)
                     /label=RSV promoter
                     /note="Rous sarcoma virus enhancer/promoter"
     rep_origin      complement(816..1404)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     CDS             complement(1578..2435)
                     /codon_start=1
                     /label=AmpR
                     /note="beta-lactamase"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRVDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPAAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     promoter        complement(2436..2540)
                     /label=AmpR promoter
     polyA_signal    complement(3393..3527)
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     CDS             complement(3952..3972)
                     /codon_start=1
                     /label=SV40 NLS
                     /note="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /translation="PKKKRKV"
     intron          complement(4102..4167)
                     /label=small t intron
                     /note="SV40 (simian virus 40) small t antigen intron"
     CDS             complement(4336..4992)
                     /codon_start=1
                     /label=CmR
                     /note="chloramphenicol acetyltransferase"
                     /translation="MEKKITGYTTVDISQWHRKEHFEAFQSVAQCTYNQTVQLDITAFL
                     KTVKKNKHKFYPAFIHILARLMNAHPEFRMAMKDGELVIWDSVHPCYTVFHEQTETFSS
                     LWSEYHDDFRQFLHIYSQDVACYGENLAYFPKGFIENMFFVSANPWVSFTSFDLNVANM
                     DNFFAPVFTMGKYYTQGDKVLMPLAIQVHHAVCDGFHVGRMLNELQQYCDEWQGGA"
ORIGIN
        1 cttggaggtg cacaccaatg tggtgaatgg tcaaatggcg tttattgtat cgagctaggc
       61 acttaaatac aatatctctg caatgcggaa ttcagtggtt cgtccaatcc atgtcagacc
      121 cgtctgttgc cttcctaata aggcacgatc gtaccacctt acttccacca atcggcatgc
      181 acggtgcttt ttctctcctt gtaaggcatg ttgctaactc atcgttacca tgttgcaaga
      241 ctacaagagt attgcataag actacatttc cccctcccta tgcaaaagcg aaactactat
      301 atcctgaggg gactcctaac cgcgtacaac cgaagccccg cttttcgcct aaacacaccc
      361 tagtcccctc agatacgcgt atatctggcc cgtacatcgc gaagcagcgc aaaacgccta
      421 accctaagca gattcttcat gcaattgtcg gtcaagcctt gccttgttgt agcttaaatt
      481 ttgctcgcgc actactcagc gacctccaac acacaagcag ggagcagata ctggcttaac
      541 tatgcggcat cagagcagat tgtactgaga gtgcaccata tgcggtgtga aataccgcac
      601 agatgcgtaa ggagaaaata ccgcatcagg cgctcttccg cttcctcgct cactgactcg
      661 ctgcgctcgg tcgttcggct gcggcgagcg gtatcagctc actcaaaggc ggtaatacgg
      721 ttatccacag aatcagggga taacgcagga aagaacatgt gagcaaaagg ccagcaaaag
      781 gccaggaacc gtaaaaaggc cgcgttgctg gcgtttttcc ataggctccg cccccctgac
      841 gagcatcaca aaaatcgacg ctcaagtcag aggtggcgaa acccgacagg actataaaga
      901 taccaggcgt ttccccctgg aagctccctc gtgcgctctc ctgttccgac cctgccgctt
      961 accggatacc tgtccgcctt tctcccttcg ggaagcgtgg cgctttctca tagctcacgc
     1021 tgtaggtatc tcagttcggt gtaggtcgtt cgctccaagc tgggctgtgt gcacgaaccc
     1081 cccgttcagc ccgaccgctg cgccttatcc ggtaactatc gtcttgagtc caacccggta
     1141 agacacgact tatcgccact ggcagcagcc actggtaaca ggattagcag agcgaggtat
     1201 gtaggcggtg ctacagagtt cttgaagtgg tggcctaact acggctacac tagaaggaca
     1261 gtatttggta tctgcgctct gctgaagcca gttaccttcg gaaaaagagt tggtagctct
     1321 tgatccggca aacaaaccac cgctggtagc ggtggttttt ttgtttgcaa gcagcagatt
     1381 acgcgcagaa aaaaaggatc tcaagaagat cctttgatct tttctacggg gtctgacgct
     1441 cagtggaacg aaaactcacg ttaagggatt ttggtcatga gattatcaaa aaggatcttc
     1501 acctagatcc ttttaaatta aaaatgaagt tttaaatcaa tctaaagtat atatgagtaa
     1561 acttggtctg acagttacca atgcttaatc agtgaggcac ctatctcagc gatctgtcta
     1621 tttcgttcat ccatagttgc ctgactcccc gtcgtgtaga taactacgat acgggagggc
     1681 ttaccatctg gccccagtgc tgcaatgata ccgcgagacc cacgctcacc ggctccagat
     1741 ttatcagcaa taaaccagcc agccggaagg gccgagcgca gaagtggtcc tgcaacttta
     1801 tccgcctcca tccagtctat taattgttgc cgggaagcta gagtaagtag ttcgccagtt
     1861 aatagtttgc gcaacgttgt tgccattgct gcaggcatcg tggtgtcacg ctcgtcgttt
     1921 ggtatggctt cattcagctc cggttcccaa cgatcaaggc gagttacatg atcccccatg
     1981 ttgtgcaaaa aagcggttag ctccttcggt cctccgatcg ttgtcagaag taagttggcc
     2041 gcagtgttat cactcatggt tatggcagca ctgcataatt ctcttactgt catgccatcc
     2101 gtaagatgct tttctgtgac tggtgagtac tcaaccaagt cattctgaga atagtgtatg
     2161 cggcgaccga gttgctcttg cccggcgtca acacgggata ataccgcgcc acatagcaga
     2221 actttaaaag tgctcatcat tggaaaacgt tcttcggggc gaaaactctc aaggatctta
     2281 ccgctgttga gatccagttc gatgtaaccc actcgtgcac ccaactgatc ttcagcatct
     2341 tttactttca ccagcgtttc tgggtgagca aaaacaggaa ggcaaaatgc cgcaaaaaag
     2401 ggaataaggg cgacacggaa atgttgaata ctcatactct tcctttttca atattattga
     2461 agcatttatc agggttattg tctcatgagc ggatacatat ttgaatgtat ttagaaaaat
     2521 aaacaaatag gggttccgcg cacatttccc cgaaaagtgc cacctgacgt ctaagaaacc
     2581 attattatca tgacattaac ctataaaaat aggcgtatca cgaggccctt tcgtcttcaa
     2641 gaattccttt gcctaattta aatgaggact taacctgtgg aaatattttg atgtgggaag
     2701 ctgttactgt taaaactgag gttattgggg taactgctat gttaaacttg cattcaggga
     2761 cacaaaaaac tcatgaaaat ggtgctggaa aacccattca agggtcaaat tttcattttt
     2821 ttgctgttgg tggggaacct ttggagctgc agggtgtgtt agcaaactac aggaccaaat
     2881 atcctgctca aactgtaacc ccaaaaaatg ctacagttga cagtcagcag atgaacactg
     2941 accacaaggc tgttttggat aaggataatg cttatccagt ggagtgctgg gttcctgatc
     3001 caagtaaaaa tgaaaacact agatattttg gaacctacac aggtggggaa aatgtgcctc
     3061 ctgttttgca cattactaac acagcaacca cagtgcttct tgatgagcag ggtgttgggc
     3121 ccttgtgcaa agctgacagc ttgtatgttt ctgctgttga catttgtggg ctgtttacca
     3181 acacttctgg aacacagcag tggaagggac ttcccagata ttttaaaatt acccttagaa
     3241 agcggtctgt gaaaaacccc tacccaattt cctttttgtt aagtgaccta attaacagga
     3301 ggacacagag ggtggatggg cagcctatga ttggaatgtc ctctcaagta gaggaggtta
     3361 gggtttatga ggacacagag gagcttcctg gggatccaga catgataaga tacattgatg
     3421 agtttggaca aaccacaact agaatgcagt gaaaaaaatg ctttatttgt gaaatttgtg
     3481 atgctattgc tttatttgta accattataa gctgcaataa acaagttaac aacaacaatt
     3541 gcattcattt tatgtttcag gttcaggggg aggtgtggga ggttttttaa agcaagtaaa
     3601 acctctacaa atgtggtatg gctgattatg atctctagtc aaggcactat acatcaaata
     3661 ttccttatta acccctttac aaattaaaaa gctaaaggta cacaattttt gagcatagtt
     3721 attaatagca gacactctat gcctgtgtgg agtaagaaaa aacagtatgt tatgattata
     3781 actgttatgc ctacttataa aggttacaga atatttttcc ataattttct tgtatagcag
     3841 tgcagctttt tcctttgtgg tgtaaatagc aaagcaagca agagttctat tactaaacac
     3901 agcatgactc aaaaaactta gcaattctga aggaaagtcc ttggggtctt ctacctttct
     3961 cttctttttt ggaggagtag aatgttgaga gtcagcagta gcctcatcat cactagatgg
     4021 catttcttct gagcaaaaca ggttttcctc attaaaggca ttccaccact gctcccattc
     4081 atcagttcca taggttggaa tctaaaatac acaaacaatt agaatcagta gtttaacaca
     4141 ttatacactt aaaaatttta tatttacctt agagctttaa atctctgtag gtagtttgtc
     4201 caattatgtc acaccacaga agtaaggttc cttcacaaag atccggcgaa tttctgccat
     4261 tcatccgctt attatcactt attcaggcgt agcaccaggc gtttaagggc accaataact
     4321 gccttaaaaa aattacgccc cgccctgcca ctcatcgcag tactgttgta attcattaag
     4381 cattctgccg acatggaagc catcacagac ggcatgatga acctgaatcg ccagcggcat
     4441 cagcaccttg tcgccttgcg tataatattt gcccatggtg aaaacggggg cgaagaagtt
     4501 gtccatattg gccacgttta aatcaaaact ggtgaaactc acccagggat tggctgagac
     4561 gaaaaacata ttctcaataa accctttagg gaaataggcc aggttttcac cgtaacacgc
     4621 cacatcttgc gaatatatgt gtagaaactg ccggaaatcg tcgtggtatt cactccagag
     4681 cgatgaaaac gtttcagttt gctcatggaa aacggtgtaa caagggtgaa cactatccca
     4741 tatcaccagc tcaccgtctt tcattgccat acggaattcc ggatgagcat tcatcaggcg
     4801 ggcaagaatg tgaataaagg ccggataaaa cttgtgctta tttttcttta cggtctttaa
     4861 aaaggccgta atatccagct gaacggtctg gttataggta cattgagcaa ctgactgaaa
     4921 tgcctcaaaa tgttctttac gatgccattg ggatatatca acggtggtat atccagtgat
     4981 ttttttctcc attttagctt ccttagctcc tgaaaatctc gccaag
//
