LOCUS       62056_19080        4186 bp DNA     circular SYN 01-JAN-1980
DEFINITION  synthetic circular DNA.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 4186)
  AUTHORS   .
  TITLE     Direct Submission
FEATURES             Location/Qualifiers
     source          1..4186
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     CDS             65..856
                     /codon_start=1
                     /label=NeoR/KanR
                     /note="aminoglycoside phosphotransferase"
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKASMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     rep_origin      1030..1618
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     protein_bind    1906..1927
                     /label=CAP binding site
                     /note="CAP binding activates transcription in the presence
                     of cAMP."
     promoter        1942..1972
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    1980..1996
                     /label=lac operator
                     /note="The lac repressor binds to the lac operator to
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     2004..2020
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     promoter        2165..2426
                     /label=RSV promoter
                     /note="Rous sarcoma virus enhancer/promoter"
     CDS             2555..2902
                     /codon_start=1
                     /label=Rev
                     /note="human immunodeficiency virus 1 (HIV-1) Rev protein"
                     /translation="MAGRSGDSDEDLLKAVRLIKFLYQSNPPPNPEGTRQARRNRRRRW
                     RERQRQIHSISERILSTYLGRSAEPVPLQLPPLERLTLDCNEDCGTSGTQGVGSPQILV
                     ESPTILESGAKE"
     primer_bind     complement(2989..3005)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     rep_origin      3337..3792
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow
                     indicates direction of (+) strand synthesis"
     promoter        4074..4178
                     /label=AmpR promoter
ORIGIN
        1 tcaacatttc cgtgtcgccc ttattccctt ttttgcggca ttttgccttc ctgtttttgc
       61 tcacatgatt gaacaagatg gattgcacgc aggttctccg gccgcttggg tggagaggct
      121 attcggctat gactgggcac aacagacaat cggctgctct gatgccgccg tgttccggct
      181 gtcagcgcag gggcgcccgg ttctttttgt caagaccgac ctgtccggtg ccctgaatga
      241 actgcaagac gaggcagcgc ggctatcgtg gctggccacg acgggcgttc cttgcgcagc
      301 tgtgctcgac gttgtcactg aagcgggaag ggactggctg ctattgggcg aagtgccggg
      361 gcaggatctc ctgtcatctc accttgctcc tgccgagaaa gtatccatca tggctgatgc
      421 aatgcggcgg ctgcatacgc ttgatccggc tacctgccca ttcgaccacc aagcgaaaca
      481 tcgcatcgag cgagcacgta ctcggatgga agccggtctt gtcgatcagg atgatctgga
      541 cgaagagcat caggggctcg cgccagccga actgttcgcc aggctcaagg cgagcatgcc
      601 cgacggcgag gatctcgtcg tgacccatgg cgatgcctgc ttgccgaata tcatggtgga
      661 aaatggccgc ttttctggat tcatcgactg tggccggctg ggtgtggcgg accgctatca
      721 ggacatagcg ttggctaccc gtgatattgc tgaagagctt ggcggcgaat gggctgaccg
      781 cttcctcgtg ctttacggta tcgccgctcc cgattcgcag cgcatcgcct tctatcgcct
      841 tcttgacgag ttcttctgac tgtcagacca agtttactca tatatacttt agattgattt
      901 aaaacttcat ttttaattta aaaggatcta ggtgaagatc ctttttgata atctcatgac
      961 caaaatccct taacgtgagt tttcgttcca ctgagcgtca gaccccgtag aaaagatcaa
     1021 aggatcttct tgagatcctt tttttctgcg cgtaatctgc tgcttgcaaa caaaaaaacc
     1081 accgctacca gcggtggttt gtttgccgga tcaagagcta ccaactcttt ttccgaaggt
     1141 aactggcttc agcagagcgc agataccaaa tactgttctt ctagtgtagc cgtagttagg
     1201 ccaccacttc aagaactctg tagcaccgcc tacatacctc gctctgctaa tcctgttacc
     1261 agtggctgct gccagtggcg ataagtcgtg tcttaccggg ttggactcaa gacgatagtt
     1321 accggataag gcgcagcggt cgggctgaac ggggggttcg tgcacacagc ccagcttgga
     1381 gcgaacgacc tacaccgaac tgagatacct acagcgtgag ctatgagaaa gcgccacgct
     1441 tcccgaaggg agaaaggcgg acaggtatcc ggtaagcggc agggtcggaa caggagagcg
     1501 cacgagggag cttccagggg gaaacgcctg gtatctttat agtcctgtcg ggtttcgcca
     1561 cctctgactt gagcgtcgat ttttgtgatg ctcgtcaggg gggcggagcc tatggaaaaa
     1621 cgccagcaac gcggcctttt tacggttcct ggccttttgc tggccttttg ctcacatgtt
     1681 ctttcctgcg ttatcccctg attctgtgga taaccgtatt accgcctttg agtgagctga
     1741 taccgctcgc cgcagccgaa cgaccgagcg cagcgagtca gtgagcgagg aagcggaaga
     1801 gcgcccaata cgcaaaccgc ctctccccgc gcgttggccg attcattaat gcagctggca
     1861 cgacaggttt cccgactgga aagcgggcag tgagcgcaac gcaattaatg tgagttagct
     1921 cactcattag gcaccccagg ctttacactt tatgcttccg gctcgtatgt tgtgtggaat
     1981 tgtgagcgga taacaatttc acacaggaaa cagctatgac atgattacga attcgatgta
     2041 cgggccagat atacgcgtat ctgaggggac tagggtgtgt ttaggcgaaa agcggggctt
     2101 cggttgtacg cggttaggag tcccctcagg atatagtagt ttcgcttttg catagggagg
     2161 gggaaatgta gtcttatgca atactcttgt agtcttgcaa catggtaacg atgagttagc
     2221 aacatgcctt acaaggagag aaaaagcacc gtgcatgccg attggtggaa gtaaggtggt
     2281 acgatcgtgc cttattagga aggcaacaga cgggtctgac atggattgga cgaaccactg
     2341 aattccgcat tgcagagata ttgtatttaa gtgcctagct cgatacaata aacgccattt
     2401 gaccattcac cacattggtg tgcacctcca agctcgagct cgtttagtga accgtcagat
     2461 cgcctggaga cgccatccac gctgttttga cctccataga agacaccggg accgatccag
     2521 cctcccctcg aagctagtcg attaggcatc tcctatggca ggaagaagcg gagacagcga
     2581 cgaagacctc ctcaaggcag tcagactcat caagtttctc tatcaaagca acccacctcc
     2641 caatcccgag gggacccgac aggcccgaag gaatagaaga agaaggtgga gagagagaca
     2701 gagacagatc cattcgatta gtgaacggat ccttagcact tatctgggac gatctgcgga
     2761 gcctgtgcct cttcagctac caccgcttga gagacttact cttgattgta acgaggattg
     2821 tggaacttct gggacgcagg gggtgggaag ccctcaaata ttggtggaat ctcctacaat
     2881 attggagtca ggagctaaag aatagtgctg ttagcttgct caatgccaca gctatagcag
     2941 tagctgaggg gacagatagg gttatagaag tagtacaaga agcttggcac tggccgtcgt
     3001 tttacaacgt cgtgatctga gcctgggaga tctctggcta actagggaac ccactgctta
     3061 agcctcaata aagcttgcct tgagtgcttc aagtagtgtg tgcccgtctg ttgtgtgact
     3121 ctggtaacta gagatcagga aaaccctggc gttacccaac ttaatcgcct tgcagcacat
     3181 ccccctttcg ccagctggcg taatagcgaa gaggcccgca ccgatcgccc ttcccaacag
     3241 ttgcgcagcc tgaatggcga atggcgcctg atgcggtatt ttctccttac gcatctgtgc
     3301 ggtatttcac accgcatacg tcaaagcaac catagtacgc gccctgtagc ggcgcattaa
     3361 gcgcggcggg tgtggtggtt acgcgcagcg tgaccgctac acttgccagc gccctagcgc
     3421 ccgctccttt cgctttcttc ccttcctttc tcgccacgtt cgccggcttt ccccgtcaag
     3481 ctctaaatcg ggggctccct ttagggttcc gatttagtgc tttacggcac ctcgacccca
     3541 aaaaacttga tttgggtgat ggttcacgta gtgggccatc gccctgatag acggtttttc
     3601 gccctttgac gttggagtcc acgttcttta atagtggact cttgttccaa actggaacaa
     3661 cactcaaccc tatctcgggc tattcttttg atttataagg gattttgccg atttcggcct
     3721 attggttaaa aaatgagctg atttaacaaa aatttaacgc gaattttaac aaaatattaa
     3781 cgtttacaat tttatggtgc actctcagta caatctgctc tgatgccgca tagttaagcc
     3841 agccccgaca cccgccaaca cccgctgacg cgccctgacg ggcttgtctg ctcccggcat
     3901 ccgcttacag acaagctgtg accgtctccg ggagctgcat gtgtcagagg ttttcaccgt
     3961 catcaccgaa acgcgcgaga cgaaagggcc tcgtgatacg cctattttta taggttaatg
     4021 tcatgataat aatggtttct tagacgtcag gtggcacttt tcggggaaat gtgcgcggaa
     4081 cccctatttg tttatttttc taaatacatt caaatatgta tccgctcatg agacaataac
     4141 cctgataaat gcttcaataa tattgaaaaa ggaagagtat gagtat
//
