LOCUS       V010371                11553 bp    DNA     circular SYN 01-JAN-1980
DEFINITION  Exported.
ACCESSION   V010371
VERSION     V010371
KEYWORDS    pRSGC2-U6-sg-HTS6-UbiC-Cas9-2A-Puro
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
            .
REFERENCE   1  (bases 1 to 11553)
  AUTHORS   Cellecta
  TITLE     Direct Submission
REFERENCE   2  (bases 1 to 11553)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     SGRef: number: 1; type: "Journal Article"
FEATURES             Location/Qualifiers
     source          1..11553
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        6..232
                     /label="RSV promoter"
                     /note="Rous sarcoma virus enhancer/promoter"
     LTR             233..413
                     /label="5' LTR (truncated)"
                     /note="5' LTR (truncated)"
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     misc_feature    460..585
                     /label="HIV-1 Psi"
                     /note="packaging signal of human immunodeficiency virus
                     type 1"
     misc_feature    1078..1311
                     /label="RRE"
                     /note="The Rev response element (RRE) of HIV-1 allows for
                     Rev-dependent mRNA export from the nucleus to the
                     cytoplasm."
     CDS             1496..1540
                     /label="gp41 peptide"
                     /note="antigenic peptide corresponding to amino acids 655
                     to 669 of the HIV envelope protein gp41 (Lutje Hulsik et
                     al., 2013)"
     CDS             1689..1730
                     /note="Protein Tat from Human immunodeficiency virus type 1
                     group M subtype B (isolate WMJ22). Accession#: P12509"
                     /label="Protein Tat"
     promoter        1819..2059
                     /label="U6 promoter"
                     /note="RNA polymerase III promoter for human U6 snRNA"
     misc_feature    2065..2094
                     /label="cloning site"
                     /note="cloning site"
                     /note="sgRNA cloning site"
     misc_feature    2122..2237
                     /label="cPPT/CTS"
                     /note="central polypurine tract and central termination
                     sequence of HIV-1 (lacking the first T)"
     promoter        2303..2702
                     /label="UbC promoter"
                     /note="human ubiquitin C promoter"
     CDS             2721..2741
                     /label="SV40 NLS"
                     /note="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
     CDS             2745..2765
                     /label="SV40 NLS"
                     /note="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
     CDS             2769..2789
                     /label="SV40 NLS"
                     /note="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
     CDS             2796..6896
                     /label="Cas9"
                     /note="Cas9 (Csn1) endonuclease from the Streptococcus
                     pyogenes Type II CRISPR/Cas system"
     CDS             6897..6944
                     /codon_start=1
                     /product="bipartite nuclear localization signal from
                     nucleoplasmin"
                     /label="bipartite nuclear localization signal from nucl"
                     /note="nucleoplasmin NLS"
                     /translation="KRPAATKKAGQAKKKK"
     CDS             6960..7013
                     /label="T2A"
                     /note="2A peptide from Thosea asigna virus capsid protein"
     CDS             7020..7616
                     /label="PuroR"
                     /note="puromycin N-acetyltransferase"
     misc_feature    7632..8220
                     /label="WPRE"
                     /note="woodchuck hepatitis virus posttranscriptional
                     regulatory element"
     LTR             8294..8527
                     /label="3' LTR (Delta-U3)"
                     /note="self-inactivating 3' long terminal repeat (LTR) from
                     HIV-1"
     polyA_signal    8599..8733
                     /label="SV40 poly(A) signal"
                     /note="SV40 polyadenylation signal"
     rep_origin      8760..8895
                     /label="SV40 ori"
                     /note="SV40 origin of replication"
     promoter        complement(8976..9006)
                     /label="lac promoter"
                     /note="promoter for the E. coli lac operon"
     protein_bind    complement(9021..9042)
                     /label="CAP binding site"
                     /note="CAP binding activates transcription in the presence
                     of cAMP."
     rep_origin      complement(9330..9918)
                     /direction=LEFT
                     /label="ori"
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
                     replication"
     CDS             complement(10092..10949)
                     /label="AmpR"
                     /note="beta-lactamase"
     promoter        complement(10950..11054)
                     /label="AmpR promoter"
     primer_bind     11528..11544
                     /label="M13 fwd"
                     /note="common sequencing primer, one of multiple similar
                     variants"
ORIGIN
        1 acgcgtgtag tcttatgcaa tactcttgta gtcttgcaac atggtaacga tgagttagca
       61 acatgcctta caaggagaga aaaagcaccg tgcatgccga ttggtggaag taaggtggta
      121 cgatcgtgcc ttattaggaa ggcaacagac gggtctgaca tggattggac gaaccactga
      181 attgccgcat tgcagagata ttgtatttaa gtgcctagct cgatacataa acgggtctct
      241 ctggttagac cagatctgag cctgggagct ctctggctaa ctagggaacc cactgcttaa
      301 gcctcaataa agcttgcctt gagtgcttca agtagtgtgt gcccgtctgt tgtgtgactc
      361 tggtaactag agatccctca gaccctttta gtcagtgtgg aaaatctcta gcagtggcgc
      421 ccgaacaggg acttgaaagc gaaagggaaa ccagaggagc tctctcgacg caggactcgg
      481 cttgctgaag cgcgcacggc aagaggcgag gggcggcgac tggtgagtac gccaaaaatt
      541 ttgactagcg gaggctagaa ggagagagat gggtgcgaga gcgtcagtat taagcggggg
      601 agaattagat cgcgatggga aaaaattcgg ttaaggccag ggggaaagaa aaaatataaa
      661 ttaaaacata tagtatgggc aagcagggag ctagaacgat tcgcagttaa tcctggcctg
      721 ttagaaacat cagaaggctg tagacaaata ctgggacagc tacaaccatc ccttcagaca
      781 ggatcagaag aacttagatc attatataat acagtagcaa ccctctattg tgtgcatcaa
      841 aggatagaga taaaagacac caaggaagct ttagacaaga tagaggaaga gcaaaacaaa
      901 agtaagacca ccgcacagca agcggccact gatcttcaga cctggaggag gagatatgag
      961 ggacaattgg agaagtgaat tatataaata taaagtagta aaaattgaac cattaggagt
     1021 agcacccacc aaggcaaaga gaagagtggt gcagagagaa aaaagagcag tgggaatagg
     1081 agctttgttc cttgggttct tgggagcagc aggaagcact atgggcgcag cgtcaatgac
     1141 gctgacggta caggccagac aattattgtc tggtatagtg cagcagcaga acaatttgct
     1201 gagggctatt gaggcgcaac agcatctgtt gcaactcaca gtctggggca tcaagcagct
     1261 ccaggcaaga atcctggctg tggaaagata cctaaaggat caacagctcc tggggatttg
     1321 gggttgctct ggaaaactca tttgcaccac tgctgtgcct tggaatgcta gttggagtaa
     1381 taaatctctg gaacagattt ggaatcacac gacctggatg gagtgggaca gagaaattaa
     1441 caattacaca agcttaatac actccttaat tgaagaatcg caaaaccagc aagaaaagaa
     1501 tgaacaagaa ttattggaat tagataaatg ggcaagtttg tggaattggt ttaacataac
     1561 aaattggctg tggtatataa aattattcat aatgatagta ggaggcttgg taggtttaag
     1621 aatagttttt gctgtacttt ctatagtgaa tagagttagg cagggatatt caccattatc
     1681 gtttcagacc cacctcccaa ccccgagggg acccgacagg cccgaaggaa tagaagaaga
     1741 aggtggagag agagacagag acagatccat tcgattagtg aacggatctc gacggtatcg
     1801 atggccgccc ccttcaccga gggcctattt cccatgattc cttcatattt gcatatacga
     1861 tacaaggctg ttagagagat aattggaatt aatttgactg taaacacaaa gatattagta
     1921 caaaatacgt gacgtagaaa gtaataattt cttgggtagt ttgcagtttt aaaattatgt
     1981 tttaaaatgg actatcatat gcttaccgta acttgaaagt atttcgattt cttggcttta
     2041 tatatcttgt ggaaaggacg aaacaccgga gtcttctttt ttgaagacac ttcggactgt
     2101 agaactctga acctcgagca atttaaaaga aaagggggga ttggggggta cagtgcaggg
     2161 gaaagaatag tagacataat agcaacagac atacaaacta aagaattaca aaaacaaatt
     2221 acaaaaattc aaaatttctg cgttgttgtc ggtgctcgtt ctctgctctt cacgctactg
     2281 aattcatcac cggttcttcg aaggcctccg cgccgggttt tggcgcctcc cgcgggcgcc
     2341 cccctcctca cggcgagcgc tgccacgtca gacgaagggc gcagcgagcg tcctgatcct
     2401 tccgcccgga cgctcaggac agcggcccgc tgctcataag actcggcctt agaaccccag
     2461 tatcagcaga aggacatttt aggacgggac ttgggtgact ctagggcact ggttttcttt
     2521 ccagagagcg gaacaggcga ggaaaagtag tcccttctcg gcgattctgc ggagggatct
     2581 ccgtggggcg gtgaacgccg atgattatat aaggacgcgc cgggtgtggc acagctagtt
     2641 ccgtcgcagc cgggatttgg gtcgcggttc ttgtttgtgg atcgctgtga tcgtcacttg
     2701 gtctagccgc caccatggcc ccaaaaaaga agagaaaggt agatccaaaa aagaagagaa
     2761 aggtagatcc aaaaaagaag agaaaggtag gtggagacaa gaagtacagc atcggcctgg
     2821 acatcggcac caactctgtg ggctgggccg tgatcaccga cgagtacaag gtgcccagca
     2881 agaaattcaa ggtgctgggc aacaccgacc ggcacagcat caagaagaac ctgatcggag
     2941 ccctgctgtt cgacagcggc gaaacagccg aggccacccg gctgaagaga accgccagaa
     3001 gaagatacac cagacggaag aaccggatct gctatctgca agagatcttc agcaacgaga
     3061 tggccaaggt ggacgacagc ttcttccaca gactggaaga gtccttcctg gtggaagagg
     3121 ataagaagca cgagcggcac cccatcttcg gcaacatcgt ggacgaggtg gcctaccacg
     3181 agaagtaccc caccatctac cacctgagaa agaaactggt ggacagcacc gacaaggccg
     3241 acctgcggct gatctatctg gccctggccc acatgatcaa gttccggggc cacttcctga
     3301 tcgagggcga cctgaacccc gacaacagcg acgtggacaa gctgttcatc cagctggtgc
     3361 agacctacaa ccagctgttc gaggaaaacc ccatcaacgc cagcggcgtg gacgccaagg
     3421 ccatcctgtc tgccagactg agcaagagca gacggctgga aaatctgatc gcccagctgc
     3481 ccggcgagaa gaagaatggc ctgttcggaa acctgattgc cctgagcctg ggcctgaccc
     3541 ccaacttcaa gagcaacttc gacctggccg aggatgccaa actgcagctg agcaaggaca
     3601 cctacgacga cgacctggac aacctgctgg cccagatcgg cgaccagtac gccgacctgt
     3661 ttctggccgc caagaacctg tccgacgcca tcctgctgag cgacatcctg agagtgaaca
     3721 ccgagatcac caaggccccc ctgagcgcct ctatgatcaa gagatacgac gagcaccacc
     3781 aggacctgac cctgctgaaa gctctcgtgc ggcagcagct gcctgagaag tacaaagaga
     3841 ttttcttcga ccagagcaag aacggctacg ccggctacat tgacggcgga gccagccagg
     3901 aagagttcta caagttcatc aagcccatcc tggaaaagat ggacggcacc gaggaactgc
     3961 tcgtgaagct gaacagagag gacctgctgc ggaagcagcg gaccttcgac aacggcagca
     4021 tcccccacca gatccacctg ggagagctgc acgccattct gcggcggcag gaagattttt
     4081 acccattcct gaaggacaac cgggaaaaga tcgagaagat cctgaccttc cgcatcccct
     4141 actacgtggg ccctctggcc aggggaaaca gcagattcgc ctggatgacc agaaagagcg
     4201 aggaaaccat caccccctgg aacttcgagg aagtggtgga caagggcgct tccgcccaga
     4261 gcttcatcga gcggatgacc aacttcgata agaacctgcc caacgagaag gtgctgccca
     4321 agcacagcct gctgtacgag tacttcaccg tgtataacga gctgaccaaa gtgaaatacg
     4381 tgaccgaggg aatgagaaag cccgccttcc tgagcggcga gcagaaaaag gccatcgtgg
     4441 acctgctgtt caagaccaac cggaaagtga ccgtgaagca gctgaaagag gactacttca
     4501 agaaaatcga gtgcttcgac tccgtggaaa tctccggcgt ggaagatcgg ttcaacgcct
     4561 ccctgggcac ataccacgat ctgctgaaaa ttatcaagga caaggacttc ctggacaatg
     4621 aggaaaacga ggacattctg gaagatatcg tgctgaccct gacactgttt gaggacagag
     4681 agatgatcga ggaacggctg aaaacctatg cccacctgtt cgacgacaaa gtgatgaagc
     4741 agctgaagcg gcggagatac accggctggg gcaggctgag ccggaagctg atcaacggca
     4801 tccgggacaa gcagtccggc aagacaatcc tggatttcct gaagtccgac ggcttcgcca
     4861 acagaaactt catgcagctg atccacgacg acagcctgac ctttaaagag gacatccaga
     4921 aagcccaggt gtccggccag ggcgatagcc tgcacgagca cattgccaat ctggccggca
     4981 gccccgccat taagaagggc atcctgcaga cagtgaaggt ggtggacgag ctcgtgaaag
     5041 tgatgggccg gcacaagccc gagaacatcg tgatcgaaat ggccagagag aaccagacca
     5101 cccagaaggg acagaagaac agccgcgaga gaatgaagcg gatcgaagag ggcatcaaag
     5161 agctgggcag ccagatcctg aaagaacacc ccgtggaaaa cacccagctg cagaacgaga
     5221 agctgtacct gtactacctg cagaatgggc gggatatgta cgtggaccag gaactggaca
     5281 tcaaccggct gtccgactac gatgtggacc atatcgtgcc tcagagcttt ctgaaggacg
     5341 actccatcga caacaaggtg ctgaccagaa gcgacaagaa ccggggcaag agcgacaacg
     5401 tgccctccga agaggtcgtg aagaagatga agaactactg gcggcagctg ctgaacgcca
     5461 agctgattac ccagagaaag ttcgacaatc tgaccaaggc cgagagaggc ggcctgagcg
     5521 aactggataa ggccggcttc atcaagagac agctggtgga aacccggcag atcacaaagc
     5581 acgtggcaca gatcctggac tcccggatga acactaagta cgacgagaat gacaagctga
     5641 tccgggaagt gaaagtgatc accctgaagt ccaagctggt gtccgatttc cggaaggatt
     5701 tccagtttta caaagtgcgc gagatcaaca actaccacca cgcccacgac gcctacctga
     5761 acgccgtcgt gggaaccgcc ctgatcaaaa agtaccctaa gctggaaagc gagttcgtgt
     5821 acggcgacta caaggtgtac gacgtgcgga agatgatcgc caagagcgag caggaaatcg
     5881 gcaaggctac cgccaagtac ttcttctaca gcaacatcat gaactttttc aagaccgaga
     5941 ttaccctggc caacggcgag atccggaagc ggcctctgat cgagacaaac ggcgaaaccg
     6001 gggagatcgt gtgggataag ggccgggatt ttgccaccgt gcggaaagtg ctgagcatgc
     6061 cccaagtgaa tatcgtgaaa aagaccgagg tgcagacagg cggcttcagc aaagagtcta
     6121 tcctgcccaa gaggaacagc gataagctga tcgccagaaa gaaggactgg gaccctaaga
     6181 agtacggcgg cttcgacagc cccaccgtgg cctattctgt gctggtggtg gccaaagtgg
     6241 aaaagggcaa gtccaagaaa ctgaagagtg tgaaagagct gctggggatc accatcatgg
     6301 aaagaagcag cttcgagaag aatcccatcg actttctgga agccaagggc tacaaagaag
     6361 tgaaaaagga cctgatcatc aagctgccta agtactccct gttcgagctg gaaaacggcc
     6421 ggaagagaat gctggcctct gccggcgaac tgcagaaggg aaacgaactg gccctgccct
     6481 ccaaatatgt gaacttcctg tacctggcca gccactatga gaagctgaag ggctcccccg
     6541 aggataatga gcagaaacag ctgtttgtgg aacagcacaa gcactacctg gacgagatca
     6601 tcgagcagat cagcgagttc tccaagagag tgatcctggc cgacgctaat ctggacaaag
     6661 tgctgtccgc ctacaacaag caccgggata agcccatcag agagcaggcc gagaatatca
     6721 tccacctgtt taccctgacc aatctgggag cccctgccgc cttcaagtac tttgacacca
     6781 ccatcgaccg gaagaggtac accagcacca aagaggtgct ggacgccacc ctgatccacc
     6841 agagcatcac cggcctgtac gagacacgga tcgacctgtc tcagctggga ggcgacaaaa
     6901 ggccggcggc cacgaaaaag gccggccagg caaaaaagaa aaaggcggcc gcaggatccg
     6961 agggcagagg aagccttcta acatgcggtg acgtggagga gaatcccggc ccttccggga
     7021 tgaccgagta caagcccacg gtgcgcctcg ccacccgcga cgacgtcccc agggccgtac
     7081 gcaccctcgc cgccgcgttc gccgactacc ccgccacgcg ccacaccgtc gatccggacc
     7141 gccacatcga gcgggtcacc gagctgcaag aactcttcct cacgcgcgtc gggctcgaca
     7201 tcggcaaggt gtgggtcgcg gacgacggcg ccgcggtggc ggtctggacc acgccggaga
     7261 gcgtcgaagc gggggcggtg ttcgccgaga tcggcccgcg catggccgag ttgagcggtt
     7321 cccggctggc cgcgcagcaa cagatggaag gcctcctggc gccgcaccgg cccaaggagc
     7381 ccgcgtggtt cctggccacc gtcggcgtct cgcccgacca ccagggcaag ggtctgggca
     7441 gcgccgtcgt gctccccgga gtggaggcgg ccgagcgcgc cggggtgccc gccttcctgg
     7501 agacctccgc gccccgcaac ctccccttct acgagcggct cggcttcacc gtcaccgccg
     7561 acgtcgaggt gcccgaagga ccgcgcacct ggtgcatgac ccgcaagccc ggtgcctgaa
     7621 tctaggtcga caatcaacct ctggattaca aaatttgtga aagattgact ggtattctta
     7681 actatgttgc tccttttacg ctatgtggat acgctgcttt aatgcctttg tatcatgcta
     7741 ttgcttcccg tatggctttc attttctcct ccttgtataa atcctggttg ctgtctcttt
     7801 atgaggagtt gtggcccgtt gtcaggcaac gtggcgtggt gtgcactgtg tttgctgacg
     7861 caacccccac tggttggggc attgccacca cctgtcagct cctttccggg actttcgctt
     7921 tccccctccc tattgccacg gcggaactca tcgccgcctg ccttgcccgc tgctggacag
     7981 gggctcggct gttgggcact gacaattccg tggtgttgtc ggggaaatca tcgtcctttc
     8041 cttggctgct cgcctgtgtt gccacctgga ttctgcgcgg gacgtccttc tgctacgtcc
     8101 cttcggccct caatccagcg gaccttcctt cccgcggcct gctgccggct ctgcggcctc
     8161 ttccgcgtct ccgccttcgc cctcagacga gtcggatctc tctttgggcc gcctccccgc
     8221 ctggtacctt taagaccaat gacttacaag gcagctgtag atcttagcca ctttttaaaa
     8281 gaaaaggggg gactggaagg gctaattcac tcccaacgaa gataagatct gctttttgct
     8341 tgtactgggt ctctctggtt agaccagatc tgagcctggg agctctctgg ctaactaggg
     8401 aacccactgc ttaagcctca ataaagcttg ccttgagtgc ttcaagtagt gtgtgcccgt
     8461 ctgttgtgtg actctggtaa ctagagatcc ctcagaccct tttagtcagt gtggaaaatc
     8521 tctagcagta gtagttcatg tcatcttatt attcagtatt tataacttgc aaagaaatga
     8581 atatcagaga gtgagaggaa cttgtttatt gcagcttata atggttacaa ataaagcaat
     8641 agcatcacaa atttcacaaa taaagcattt ttttcactgc attctagttg tggtttgtcc
     8701 aaactcatca atgtatctta tcatgtctgg ctctagctat cccgccccta actccgccca
     8761 tcccgcccct aactccgccc agttccgccc attctccgcc ccatggctga ctaatttttt
     8821 ttatttatgc agaggccgag gccgcctcgg cctctgagct attccagaag tagtgaggag
     8881 gcttttttgg aggcctagac ttttgcagag accaaattcg taatcatgtc atagctgttt
     8941 cctgtgtgaa attgttatcc gctcacaatt ccacacaaca tacgagccgg aagcataaag
     9001 tgtaaagcct ggggtgccta atgagtgagc taactcacat taattgcgtt gcgctcactg
     9061 cccgctttcc agtcgggaaa cctgtcgtgc cagctgcatt aatgaatcgg ccaacgcgcg
     9121 gggagaggcg gtttgcgtat tgggcgctct tccgcttcct cgctcactga ctcgctgcgc
     9181 tcggtcgttc ggctgcggcg agcggtatca gctcactcaa aggcggtaat acggttatcc
     9241 acagaatcag gggataacgc aggaaagaac atgtgagcaa aaggccagca aaaggccagg
     9301 aaccgtaaaa aggccgcgtt gctggcgttt ttccataggc tccgcccccc tgacgagcat
     9361 cacaaaaatc gacgctcaag tcagaggtgg cgaaacccga caggactata aagataccag
     9421 gcgtttcccc ctggaagctc cctcgtgcgc tctcctgttc cgaccctgcc gcttaccgga
     9481 tacctgtccg cctttctccc ttcgggaagc gtggcgcttt ctcatagctc acgctgtagg
     9541 tatctcagtt cggtgtaggt cgttcgctcc aagctgggct gtgtgcacga accccccgtt
     9601 cagcccgacc gctgcgcctt atccggtaac tatcgtcttg agtccaaccc ggtaagacac
     9661 gacttatcgc cactggcagc agccactggt aacaggatta gcagagcgag gtatgtaggc
     9721 ggtgctacag agttcttgaa gtggtggcct aactacggct acactagaag aacagtattt
     9781 ggtatctgcg ctctgctgaa gccagttacc ttcggaaaaa gagttggtag ctcttgatcc
     9841 ggcaaacaaa ccaccgctgg tagcggtggt ttttttgttt gcaagcagca gattacgcgc
     9901 agaaaaaaag gatctcaaga agatcctttg atcttttcta cggggtctga cgctcagtgg
     9961 aacgaaaact cacgttaagg gattttggtc atgagattat caaaaaggat cttcacctag
    10021 atccttttaa attaaaaatg aagttttaaa tcaatctaaa gtatatatga gtaaacttgg
    10081 tctgacagtt accaatgctt aatcagtgag gcacctatct cagcgatctg tctatttcgt
    10141 tcatccatag ttgcctgact ccccgtcgtg tagataacta cgatacggga gggcttacca
    10201 tctggcccca gtgctgcaat gataccgcga gacccacgct caccggctcc agatttatca
    10261 gcaataaacc agccagccgg aagggccgag cgcagaagtg gtcctgcaac tttatccgcc
    10321 tccatccagt ctattaattg ttgccgggaa gctagagtaa gtagttcgcc agttaatagt
    10381 ttgcgcaacg ttgttgccat tgctacaggc atcgtggtgt cacgctcgtc gtttggtatg
    10441 gcttcattca gctccggttc ccaacgatca aggcgagtta catgatcccc catgttgtgc
    10501 aaaaaagcgg ttagctcctt cggtcctccg atcgttgtca gaagtaagtt ggccgcagtg
    10561 ttatcactca tggttatggc agcactgcat aattctctta ctgtcatgcc atccgtaaga
    10621 tgcttttctg tgactggtga gtactcaacc aagtcattct gagaatagtg tatgcggcga
    10681 ccgagttgct cttgcccggc gtcaatacgg gataataccg cgccacatag cagaacttta
    10741 aaagtgctca tcattggaaa acgttcttcg gggcgaaaac tctcaaggat cttaccgctg
    10801 ttgagatcca gttcgatgta acccactcgt gcacccaact gatcttcagc atcttttact
    10861 ttcaccagcg tttctgggtg agcaaaaaca ggaaggcaaa atgccgcaaa aaagggaata
    10921 agggcgacac ggaaatgttg aatactcata ctcttccttt ttcaatatta ttgaagcatt
    10981 tatcagggtt attgtctcat gagcggatac atatttgaat gtatttagaa aaataaacaa
    11041 ataggggttc cgcgcacatt tccccgaaaa gtgccacctg acgtctaaga aaccattatt
    11101 atcatgacat taacctataa aaataggcgt atcacgaggc cctttcgtct cgcgcgtttc
    11161 ggtgatgacg gtgaaaacct ctgacacatg cagctcccgg agacggtcac agcttgtctg
    11221 taagcggatg ccgggagcag acaagcccgt cagggcgcgt cagcgggtgt tggcgggtgt
    11281 cggggctggc ttaactatgc ggcatcagag cagattgtac tgagagtgca ccatatgcgg
    11341 tgtgaaatac cgcacagatg cgtaaggaga aaataccgca tcaggcgcca ttcgccattc
    11401 aggctgcgca actgttggga agggcgatcg gtgcgggcct cttcgctatt acgccagctg
    11461 gcgaaagggg gatgtgctgc aaggcgatta agttgggtaa cgccagggtt ttcccagtca
    11521 cgacgttgta aaacgacggc cagtgccaag ctg
//
