LOCUS       V010372                11505 bp    DNA     circular SYN 01-JAN-1980
DEFINITION  Exported.
ACCESSION   V010372
VERSION     V010372
KEYWORDS    pRSGC1-U6-sg-HTS6-CMV-Cas9-2A-Puro
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
            .
REFERENCE   1  (bases 1 to 11505)
  AUTHORS   Cellecta
  TITLE     Direct Submission
REFERENCE   2  (bases 1 to 11505)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     SGRef: number: 1; type: "Journal Article"
FEATURES             Location/Qualifiers
     source          1..11505
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        6..232
                     /label="RSV promoter"
                     /note="Rous sarcoma virus enhancer/promoter"
     LTR             233..413
                     /label="5' LTR (truncated)"
                     /note="5' LTR (truncated)"
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     misc_feature    460..585
                     /label="HIV-1 Psi"
                     /note="packaging signal of human immunodeficiency virus
                     type 1"
     misc_feature    1078..1311
                     /label="RRE"
                     /note="The Rev response element (RRE) of HIV-1 allows for
                     Rev-dependent mRNA export from the nucleus to the
                     cytoplasm."
     CDS             1496..1540
                     /label="gp41 peptide"
                     /note="antigenic peptide corresponding to amino acids 655
                     to 669 of the HIV envelope protein gp41 (Lutje Hulsik et
                     al., 2013)"
     CDS             1689..1730
                     /note="Protein Tat from Human immunodeficiency virus type 1
                     group M subtype B (isolate WMJ22). Accession#: P12509"
                     /label="Protein Tat"
     promoter        1819..2059
                     /label="U6 promoter"
                     /note="RNA polymerase III promoter for human U6 snRNA"
     misc_feature    2065..2094
                     /label="cloning site"
                     /note="cloning site"
                     /note="sgRNA cloning site"
     misc_feature    2122..2237
                     /label="cPPT/CTS"
                     /note="central polypurine tract and central termination
                     sequence of HIV-1 (lacking the first T)"
     promoter        2386..2589
                     /label="CMV promoter"
                     /note="human cytomegalovirus (CMV) immediate early
                     promoter"
     CDS             2673..2693
                     /label="SV40 NLS"
                     /note="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
     CDS             2697..2717
                     /label="SV40 NLS"
                     /note="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
     CDS             2721..2741
                     /label="SV40 NLS"
                     /note="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
     CDS             2748..6848
                     /label="Cas9"
                     /note="Cas9 (Csn1) endonuclease from the Streptococcus
                     pyogenes Type II CRISPR/Cas system"
     CDS             6849..6896
                     /codon_start=1
                     /product="bipartite nuclear localization signal from
                     nucleoplasmin"
                     /label="bipartite nuclear localization signal from nucl"
                     /note="nucleoplasmin NLS"
                     /translation="KRPAATKKAGQAKKKK"
     CDS             6912..6965
                     /label="T2A"
                     /note="2A peptide from Thosea asigna virus capsid protein"
     CDS             6972..7568
                     /label="PuroR"
                     /note="puromycin N-acetyltransferase"
     misc_feature    7584..8172
                     /label="WPRE"
                     /note="woodchuck hepatitis virus posttranscriptional
                     regulatory element"
     LTR             8246..8479
                     /label="3' LTR (Delta-U3)"
                     /note="self-inactivating 3' long terminal repeat (LTR) from
                     HIV-1"
     polyA_signal    8551..8685
                     /label="SV40 poly(A) signal"
                     /note="SV40 polyadenylation signal"
     rep_origin      8712..8847
                     /label="SV40 ori"
                     /note="SV40 origin of replication"
     promoter        complement(8928..8958)
                     /label="lac promoter"
                     /note="promoter for the E. coli lac operon"
     protein_bind    complement(8973..8994)
                     /label="CAP binding site"
                     /note="CAP binding activates transcription in the presence
                     of cAMP."
     rep_origin      complement(9282..9870)
                     /direction=LEFT
                     /label="ori"
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
                     replication"
     CDS             complement(10044..10901)
                     /label="AmpR"
                     /note="beta-lactamase"
     promoter        complement(10902..11006)
                     /label="AmpR promoter"
     primer_bind     11480..11496
                     /label="M13 fwd"
                     /note="common sequencing primer, one of multiple similar
                     variants"
ORIGIN
        1 acgcgtgtag tcttatgcaa tactcttgta gtcttgcaac atggtaacga tgagttagca
       61 acatgcctta caaggagaga aaaagcaccg tgcatgccga ttggtggaag taaggtggta
      121 cgatcgtgcc ttattaggaa ggcaacagac gggtctgaca tggattggac gaaccactga
      181 attgccgcat tgcagagata ttgtatttaa gtgcctagct cgatacataa acgggtctct
      241 ctggttagac cagatctgag cctgggagct ctctggctaa ctagggaacc cactgcttaa
      301 gcctcaataa agcttgcctt gagtgcttca agtagtgtgt gcccgtctgt tgtgtgactc
      361 tggtaactag agatccctca gaccctttta gtcagtgtgg aaaatctcta gcagtggcgc
      421 ccgaacaggg acttgaaagc gaaagggaaa ccagaggagc tctctcgacg caggactcgg
      481 cttgctgaag cgcgcacggc aagaggcgag gggcggcgac tggtgagtac gccaaaaatt
      541 ttgactagcg gaggctagaa ggagagagat gggtgcgaga gcgtcagtat taagcggggg
      601 agaattagat cgcgatggga aaaaattcgg ttaaggccag ggggaaagaa aaaatataaa
      661 ttaaaacata tagtatgggc aagcagggag ctagaacgat tcgcagttaa tcctggcctg
      721 ttagaaacat cagaaggctg tagacaaata ctgggacagc tacaaccatc ccttcagaca
      781 ggatcagaag aacttagatc attatataat acagtagcaa ccctctattg tgtgcatcaa
      841 aggatagaga taaaagacac caaggaagct ttagacaaga tagaggaaga gcaaaacaaa
      901 agtaagacca ccgcacagca agcggccact gatcttcaga cctggaggag gagatatgag
      961 ggacaattgg agaagtgaat tatataaata taaagtagta aaaattgaac cattaggagt
     1021 agcacccacc aaggcaaaga gaagagtggt gcagagagaa aaaagagcag tgggaatagg
     1081 agctttgttc cttgggttct tgggagcagc aggaagcact atgggcgcag cgtcaatgac
     1141 gctgacggta caggccagac aattattgtc tggtatagtg cagcagcaga acaatttgct
     1201 gagggctatt gaggcgcaac agcatctgtt gcaactcaca gtctggggca tcaagcagct
     1261 ccaggcaaga atcctggctg tggaaagata cctaaaggat caacagctcc tggggatttg
     1321 gggttgctct ggaaaactca tttgcaccac tgctgtgcct tggaatgcta gttggagtaa
     1381 taaatctctg gaacagattt ggaatcacac gacctggatg gagtgggaca gagaaattaa
     1441 caattacaca agcttaatac actccttaat tgaagaatcg caaaaccagc aagaaaagaa
     1501 tgaacaagaa ttattggaat tagataaatg ggcaagtttg tggaattggt ttaacataac
     1561 aaattggctg tggtatataa aattattcat aatgatagta ggaggcttgg taggtttaag
     1621 aatagttttt gctgtacttt ctatagtgaa tagagttagg cagggatatt caccattatc
     1681 gtttcagacc cacctcccaa ccccgagggg acccgacagg cccgaaggaa tagaagaaga
     1741 aggtggagag agagacagag acagatccat tcgattagtg aacggatctc gacggtatcg
     1801 atggccgccc ccttcaccga gggcctattt cccatgattc cttcatattt gcatatacga
     1861 tacaaggctg ttagagagat aattggaatt aatttgactg taaacacaaa gatattagta
     1921 caaaatacgt gacgtagaaa gtaataattt cttgggtagt ttgcagtttt aaaattatgt
     1981 tttaaaatgg actatcatat gcttaccgta acttgaaagt atttcgattt cttggcttta
     2041 tatatcttgt ggaaaggacg aaacaccgga gtcttctttt ttgaagacac ttcggactgt
     2101 agaactctga acctcgagca atttaaaaga aaagggggga ttggggggta cagtgcaggg
     2161 gaaagaatag tagacataat agcaacagac atacaaacta aagaattaca aaaacaaatt
     2221 acaaaaattc aaaatttctg cgttgttgtc ggtgctcgtt ctctgctctt cacgctactg
     2281 aattcatcac cggttcttcg aatctagtat tatgcccagt acatgacctt atgggacttt
     2341 cctacttggc agtacatcta cgtattagtc atcgctatta ccatggtgat gcggttttgg
     2401 cagtacatca atgggcgtgg atagcggttt gactcacggg gatttccaag tctccacccc
     2461 attgacgcca atgggagttt gttttggcac caaaatcaac gggactttcc aaaatgtcgt
     2521 aacaactccg ccccattgac gcaaatgggc ggtaggcgtg tacggtggaa ggtttatata
     2581 agcagagctc gtttagtgaa ccgtcagatc gcctggagac gccatccacg ctgttttgac
     2641 ctccatagaa gattctagcc gccaccatgg ccccaaaaaa gaagagaaag gtagatccaa
     2701 aaaagaagag aaaggtagat ccaaaaaaga agagaaaggt aggtggagac aagaagtaca
     2761 gcatcggcct ggacatcggc accaactctg tgggctgggc cgtgatcacc gacgagtaca
     2821 aggtgcccag caagaaattc aaggtgctgg gcaacaccga ccggcacagc atcaagaaga
     2881 acctgatcgg agccctgctg ttcgacagcg gcgaaacagc cgaggccacc cggctgaaga
     2941 gaaccgccag aagaagatac accagacgga agaaccggat ctgctatctg caagagatct
     3001 tcagcaacga gatggccaag gtggacgaca gcttcttcca cagactggaa gagtccttcc
     3061 tggtggaaga ggataagaag cacgagcggc accccatctt cggcaacatc gtggacgagg
     3121 tggcctacca cgagaagtac cccaccatct accacctgag aaagaaactg gtggacagca
     3181 ccgacaaggc cgacctgcgg ctgatctatc tggccctggc ccacatgatc aagttccggg
     3241 gccacttcct gatcgagggc gacctgaacc ccgacaacag cgacgtggac aagctgttca
     3301 tccagctggt gcagacctac aaccagctgt tcgaggaaaa ccccatcaac gccagcggcg
     3361 tggacgccaa ggccatcctg tctgccagac tgagcaagag cagacggctg gaaaatctga
     3421 tcgcccagct gcccggcgag aagaagaatg gcctgttcgg aaacctgatt gccctgagcc
     3481 tgggcctgac ccccaacttc aagagcaact tcgacctggc cgaggatgcc aaactgcagc
     3541 tgagcaagga cacctacgac gacgacctgg acaacctgct ggcccagatc ggcgaccagt
     3601 acgccgacct gtttctggcc gccaagaacc tgtccgacgc catcctgctg agcgacatcc
     3661 tgagagtgaa caccgagatc accaaggccc ccctgagcgc ctctatgatc aagagatacg
     3721 acgagcacca ccaggacctg accctgctga aagctctcgt gcggcagcag ctgcctgaga
     3781 agtacaaaga gattttcttc gaccagagca agaacggcta cgccggctac attgacggcg
     3841 gagccagcca ggaagagttc tacaagttca tcaagcccat cctggaaaag atggacggca
     3901 ccgaggaact gctcgtgaag ctgaacagag aggacctgct gcggaagcag cggaccttcg
     3961 acaacggcag catcccccac cagatccacc tgggagagct gcacgccatt ctgcggcggc
     4021 aggaagattt ttacccattc ctgaaggaca accgggaaaa gatcgagaag atcctgacct
     4081 tccgcatccc ctactacgtg ggccctctgg ccaggggaaa cagcagattc gcctggatga
     4141 ccagaaagag cgaggaaacc atcaccccct ggaacttcga ggaagtggtg gacaagggcg
     4201 cttccgccca gagcttcatc gagcggatga ccaacttcga taagaacctg cccaacgaga
     4261 aggtgctgcc caagcacagc ctgctgtacg agtacttcac cgtgtataac gagctgacca
     4321 aagtgaaata cgtgaccgag ggaatgagaa agcccgcctt cctgagcggc gagcagaaaa
     4381 aggccatcgt ggacctgctg ttcaagacca accggaaagt gaccgtgaag cagctgaaag
     4441 aggactactt caagaaaatc gagtgcttcg actccgtgga aatctccggc gtggaagatc
     4501 ggttcaacgc ctccctgggc acataccacg atctgctgaa aattatcaag gacaaggact
     4561 tcctggacaa tgaggaaaac gaggacattc tggaagatat cgtgctgacc ctgacactgt
     4621 ttgaggacag agagatgatc gaggaacggc tgaaaaccta tgcccacctg ttcgacgaca
     4681 aagtgatgaa gcagctgaag cggcggagat acaccggctg gggcaggctg agccggaagc
     4741 tgatcaacgg catccgggac aagcagtccg gcaagacaat cctggatttc ctgaagtccg
     4801 acggcttcgc caacagaaac ttcatgcagc tgatccacga cgacagcctg acctttaaag
     4861 aggacatcca gaaagcccag gtgtccggcc agggcgatag cctgcacgag cacattgcca
     4921 atctggccgg cagccccgcc attaagaagg gcatcctgca gacagtgaag gtggtggacg
     4981 agctcgtgaa agtgatgggc cggcacaagc ccgagaacat cgtgatcgaa atggccagag
     5041 agaaccagac cacccagaag ggacagaaga acagccgcga gagaatgaag cggatcgaag
     5101 agggcatcaa agagctgggc agccagatcc tgaaagaaca ccccgtggaa aacacccagc
     5161 tgcagaacga gaagctgtac ctgtactacc tgcagaatgg gcgggatatg tacgtggacc
     5221 aggaactgga catcaaccgg ctgtccgact acgatgtgga ccatatcgtg cctcagagct
     5281 ttctgaagga cgactccatc gacaacaagg tgctgaccag aagcgacaag aaccggggca
     5341 agagcgacaa cgtgccctcc gaagaggtcg tgaagaagat gaagaactac tggcggcagc
     5401 tgctgaacgc caagctgatt acccagagaa agttcgacaa tctgaccaag gccgagagag
     5461 gcggcctgag cgaactggat aaggccggct tcatcaagag acagctggtg gaaacccggc
     5521 agatcacaaa gcacgtggca cagatcctgg actcccggat gaacactaag tacgacgaga
     5581 atgacaagct gatccgggaa gtgaaagtga tcaccctgaa gtccaagctg gtgtccgatt
     5641 tccggaagga tttccagttt tacaaagtgc gcgagatcaa caactaccac cacgcccacg
     5701 acgcctacct gaacgccgtc gtgggaaccg ccctgatcaa aaagtaccct aagctggaaa
     5761 gcgagttcgt gtacggcgac tacaaggtgt acgacgtgcg gaagatgatc gccaagagcg
     5821 agcaggaaat cggcaaggct accgccaagt acttcttcta cagcaacatc atgaactttt
     5881 tcaagaccga gattaccctg gccaacggcg agatccggaa gcggcctctg atcgagacaa
     5941 acggcgaaac cggggagatc gtgtgggata agggccggga ttttgccacc gtgcggaaag
     6001 tgctgagcat gccccaagtg aatatcgtga aaaagaccga ggtgcagaca ggcggcttca
     6061 gcaaagagtc tatcctgccc aagaggaaca gcgataagct gatcgccaga aagaaggact
     6121 gggaccctaa gaagtacggc ggcttcgaca gccccaccgt ggcctattct gtgctggtgg
     6181 tggccaaagt ggaaaagggc aagtccaaga aactgaagag tgtgaaagag ctgctgggga
     6241 tcaccatcat ggaaagaagc agcttcgaga agaatcccat cgactttctg gaagccaagg
     6301 gctacaaaga agtgaaaaag gacctgatca tcaagctgcc taagtactcc ctgttcgagc
     6361 tggaaaacgg ccggaagaga atgctggcct ctgccggcga actgcagaag ggaaacgaac
     6421 tggccctgcc ctccaaatat gtgaacttcc tgtacctggc cagccactat gagaagctga
     6481 agggctcccc cgaggataat gagcagaaac agctgtttgt ggaacagcac aagcactacc
     6541 tggacgagat catcgagcag atcagcgagt tctccaagag agtgatcctg gccgacgcta
     6601 atctggacaa agtgctgtcc gcctacaaca agcaccggga taagcccatc agagagcagg
     6661 ccgagaatat catccacctg tttaccctga ccaatctggg agcccctgcc gccttcaagt
     6721 actttgacac caccatcgac cggaagaggt acaccagcac caaagaggtg ctggacgcca
     6781 ccctgatcca ccagagcatc accggcctgt acgagacacg gatcgacctg tctcagctgg
     6841 gaggcgacaa aaggccggcg gccacgaaaa aggccggcca ggcaaaaaag aaaaaggcgg
     6901 ccgcaggatc cgagggcaga ggaagccttc taacatgcgg tgacgtggag gagaatcccg
     6961 gcccttccgg gatgaccgag tacaagccca cggtgcgcct cgccacccgc gacgacgtcc
     7021 ccagggccgt acgcaccctc gccgccgcgt tcgccgacta ccccgccacg cgccacaccg
     7081 tcgatccgga ccgccacatc gagcgggtca ccgagctgca agaactcttc ctcacgcgcg
     7141 tcgggctcga catcggcaag gtgtgggtcg cggacgacgg cgccgcggtg gcggtctgga
     7201 ccacgccgga gagcgtcgaa gcgggggcgg tgttcgccga gatcggcccg cgcatggccg
     7261 agttgagcgg ttcccggctg gccgcgcagc aacagatgga aggcctcctg gcgccgcacc
     7321 ggcccaagga gcccgcgtgg ttcctggcca ccgtcggcgt ctcgcccgac caccagggca
     7381 agggtctggg cagcgccgtc gtgctccccg gagtggaggc ggccgagcgc gccggggtgc
     7441 ccgccttcct ggagacctcc gcgccccgca acctcccctt ctacgagcgg ctcggcttca
     7501 ccgtcaccgc cgacgtcgag gtgcccgaag gaccgcgcac ctggtgcatg acccgcaagc
     7561 ccggtgcctg aatctaggtc gacaatcaac ctctggatta caaaatttgt gaaagattga
     7621 ctggtattct taactatgtt gctcctttta cgctatgtgg atacgctgct ttaatgcctt
     7681 tgtatcatgc tattgcttcc cgtatggctt tcattttctc ctccttgtat aaatcctggt
     7741 tgctgtctct ttatgaggag ttgtggcccg ttgtcaggca acgtggcgtg gtgtgcactg
     7801 tgtttgctga cgcaaccccc actggttggg gcattgccac cacctgtcag ctcctttccg
     7861 ggactttcgc tttccccctc cctattgcca cggcggaact catcgccgcc tgccttgccc
     7921 gctgctggac aggggctcgg ctgttgggca ctgacaattc cgtggtgttg tcggggaaat
     7981 catcgtcctt tccttggctg ctcgcctgtg ttgccacctg gattctgcgc gggacgtcct
     8041 tctgctacgt cccttcggcc ctcaatccag cggaccttcc ttcccgcggc ctgctgccgg
     8101 ctctgcggcc tcttccgcgt ctccgccttc gccctcagac gagtcggatc tctctttggg
     8161 ccgcctcccc gcctggtacc tttaagacca atgacttaca aggcagctgt agatcttagc
     8221 cactttttaa aagaaaaggg gggactggaa gggctaattc actcccaacg aagataagat
     8281 ctgctttttg cttgtactgg gtctctctgg ttagaccaga tctgagcctg ggagctctct
     8341 ggctaactag ggaacccact gcttaagcct caataaagct tgccttgagt gcttcaagta
     8401 gtgtgtgccc gtctgttgtg tgactctggt aactagagat ccctcagacc cttttagtca
     8461 gtgtggaaaa tctctagcag tagtagttca tgtcatctta ttattcagta tttataactt
     8521 gcaaagaaat gaatatcaga gagtgagagg aacttgttta ttgcagctta taatggttac
     8581 aaataaagca atagcatcac aaatttcaca aataaagcat ttttttcact gcattctagt
     8641 tgtggtttgt ccaaactcat caatgtatct tatcatgtct ggctctagct atcccgcccc
     8701 taactccgcc catcccgccc ctaactccgc ccagttccgc ccattctccg ccccatggct
     8761 gactaatttt ttttatttat gcagaggccg aggccgcctc ggcctctgag ctattccaga
     8821 agtagtgagg aggctttttt ggaggcctag acttttgcag agaccaaatt cgtaatcatg
     8881 tcatagctgt ttcctgtgtg aaattgttat ccgctcacaa ttccacacaa catacgagcc
     8941 ggaagcataa agtgtaaagc ctggggtgcc taatgagtga gctaactcac attaattgcg
     9001 ttgcgctcac tgcccgcttt ccagtcggga aacctgtcgt gccagctgca ttaatgaatc
     9061 ggccaacgcg cggggagagg cggtttgcgt attgggcgct cttccgcttc ctcgctcact
     9121 gactcgctgc gctcggtcgt tcggctgcgg cgagcggtat cagctcactc aaaggcggta
     9181 atacggttat ccacagaatc aggggataac gcaggaaaga acatgtgagc aaaaggccag
     9241 caaaaggcca ggaaccgtaa aaaggccgcg ttgctggcgt ttttccatag gctccgcccc
     9301 cctgacgagc atcacaaaaa tcgacgctca agtcagaggt ggcgaaaccc gacaggacta
     9361 taaagatacc aggcgtttcc ccctggaagc tccctcgtgc gctctcctgt tccgaccctg
     9421 ccgcttaccg gatacctgtc cgcctttctc ccttcgggaa gcgtggcgct ttctcatagc
     9481 tcacgctgta ggtatctcag ttcggtgtag gtcgttcgct ccaagctggg ctgtgtgcac
     9541 gaaccccccg ttcagcccga ccgctgcgcc ttatccggta actatcgtct tgagtccaac
     9601 ccggtaagac acgacttatc gccactggca gcagccactg gtaacaggat tagcagagcg
     9661 aggtatgtag gcggtgctac agagttcttg aagtggtggc ctaactacgg ctacactaga
     9721 agaacagtat ttggtatctg cgctctgctg aagccagtta ccttcggaaa aagagttggt
     9781 agctcttgat ccggcaaaca aaccaccgct ggtagcggtg gtttttttgt ttgcaagcag
     9841 cagattacgc gcagaaaaaa aggatctcaa gaagatcctt tgatcttttc tacggggtct
     9901 gacgctcagt ggaacgaaaa ctcacgttaa gggattttgg tcatgagatt atcaaaaagg
     9961 atcttcacct agatcctttt aaattaaaaa tgaagtttta aatcaatcta aagtatatat
    10021 gagtaaactt ggtctgacag ttaccaatgc ttaatcagtg aggcacctat ctcagcgatc
    10081 tgtctatttc gttcatccat agttgcctga ctccccgtcg tgtagataac tacgatacgg
    10141 gagggcttac catctggccc cagtgctgca atgataccgc gagacccacg ctcaccggct
    10201 ccagatttat cagcaataaa ccagccagcc ggaagggccg agcgcagaag tggtcctgca
    10261 actttatccg cctccatcca gtctattaat tgttgccggg aagctagagt aagtagttcg
    10321 ccagttaata gtttgcgcaa cgttgttgcc attgctacag gcatcgtggt gtcacgctcg
    10381 tcgtttggta tggcttcatt cagctccggt tcccaacgat caaggcgagt tacatgatcc
    10441 cccatgttgt gcaaaaaagc ggttagctcc ttcggtcctc cgatcgttgt cagaagtaag
    10501 ttggccgcag tgttatcact catggttatg gcagcactgc ataattctct tactgtcatg
    10561 ccatccgtaa gatgcttttc tgtgactggt gagtactcaa ccaagtcatt ctgagaatag
    10621 tgtatgcggc gaccgagttg ctcttgcccg gcgtcaatac gggataatac cgcgccacat
    10681 agcagaactt taaaagtgct catcattgga aaacgttctt cggggcgaaa actctcaagg
    10741 atcttaccgc tgttgagatc cagttcgatg taacccactc gtgcacccaa ctgatcttca
    10801 gcatctttta ctttcaccag cgtttctggg tgagcaaaaa caggaaggca aaatgccgca
    10861 aaaaagggaa taagggcgac acggaaatgt tgaatactca tactcttcct ttttcaatat
    10921 tattgaagca tttatcaggg ttattgtctc atgagcggat acatatttga atgtatttag
    10981 aaaaataaac aaataggggt tccgcgcaca tttccccgaa aagtgccacc tgacgtctaa
    11041 gaaaccatta ttatcatgac attaacctat aaaaataggc gtatcacgag gccctttcgt
    11101 ctcgcgcgtt tcggtgatga cggtgaaaac ctctgacaca tgcagctccc ggagacggtc
    11161 acagcttgtc tgtaagcgga tgccgggagc agacaagccc gtcagggcgc gtcagcgggt
    11221 gttggcgggt gtcggggctg gcttaactat gcggcatcag agcagattgt actgagagtg
    11281 caccatatgc ggtgtgaaat accgcacaga tgcgtaagga gaaaataccg catcaggcgc
    11341 cattcgccat tcaggctgcg caactgttgg gaagggcgat cggtgcgggc ctcttcgcta
    11401 ttacgccagc tggcgaaagg gggatgtgct gcaaggcgat taagttgggt aacgccaggg
    11461 ttttcccagt cacgacgttg taaaacgacg gccagtgcca agctg
//
