LOCUS       62056_19035        6055 bp DNA     circular SYN 01-JAN-1980
DEFINITION  synthetic circular DNA.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 6055)
  AUTHORS   .
  TITLE     Direct Submission
FEATURES             Location/Qualifiers
     source          1..6055
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        20..38
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     RBS             70..92
                     /label=RBS
                     /note="efficient ribosome binding site from bacteriophage
                     T7 gene 10 (Olins and Rangwala, 1989)"
     CDS             112..129
                     /codon_start=1
                     /label=6xHis
                     /note="6xHis affinity tag"
                     /translation="HHHHHH"
     CDS             169..192
                     /codon_start=1
                     /label=Xpress(TM) tag
                     /note="Xpress(TM) epitope tag, including an enterokinase 
                     recognition and cleavage site"
                     /translation="DLYDDDDK"
     CDS             199..915
                     /codon_start=1
                     /label=ECFP
                     /note="enhanced CFP"
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTWGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYISHNVYITADKQKNGIK
                     ANFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     terminator      3470..3517
                     /label=T7 terminator
                     /note="transcription terminator for bacteriophage T7 RNA 
                     polymerase"
     rep_origin      3614..4069
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow
                     indicates direction of (+) strand synthesis"
     promoter        4095..4199
                     /label=AmpR promoter
     CDS             4200..5057
                     /codon_start=1
                     /label=AmpR
                     /note="beta-lactamase"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     rep_origin      5231..5819
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
ORIGIN
        1 gatctcgatc ccgcgaaatt aatacgactc actataggga gaccacaacg gtttccctct
       61 agaaataatt ttgtttaact ttaagaagga gatatacata tgcggggttc tcatcatcat
      121 catcatcatg gtatggctag catgactggt ggacagcaaa tgggtcggga tctgtacgac
      181 gatgacgata aggatccgat ggtgagcaag ggcgaggagc tgttcaccgg ggtggtgccc
      241 atcctggtcg agctggacgg cgacgtaaac ggccacaagt tcagcgtgtc cggcgagggc
      301 gagggcgatg ccacctacgg caagctgacc ctgaagttca tctgcaccac cggcaagctg
      361 cccgtgccct ggcccaccct cgtgaccacc ctgacctggg gcgtgcagtg cttcagccgc
      421 taccccgacc acatgaagca gcacgacttc ttcaagtccg ccatgcccga aggctacgtc
      481 caggagcgca ccatcttctt caaggacgac ggcaactaca agacccgcgc cgaggtgaag
      541 ttcgagggcg acaccctggt gaaccgcatc gagctgaagg gcatcgactt caaggaggac
      601 ggcaacatcc tggggcacaa gctggagtac aactacatca gccacaacgt ctatatcacc
      661 gccgacaagc agaagaacgg catcaaggcc aacttcaaga tccgccacaa catcgaggac
      721 ggcagcgtgc agctcgccga ccactaccag cagaacaccc ccatcggcga cggccccgtg
      781 ctgctgcccg acaaccacta cctgagcacc cagtccgccc tgagcaaaga ccccaacgag
      841 aagcgcgatc acatggtcct gctggagttc gtgaccgccg ccgggatcac tctcggcatg
      901 gacgagctgt acaagggtgg atcaggcggt actggcgggg cgcagacccc caacgccacc
      961 tcggaaggtt gccagattat acatccgccc tgggaaggtg gcatcaggta ccgtggcttg
     1021 actcgcgacc aggtgaaggc catcaacttc ctgcctgtgg actatgagat cgaatatgtg
     1081 tgccgagggg agcgcgaggt ggtggggccc aaggtgcgca aatgcctggc caacggctcc
     1141 tggacggata tggacacacc cagccgctgt gtccgaatct gctccaagtc ttatttgacc
     1201 ctggaaaatg ggaaggtttt cctgacgggt ggggacctcc cagctctgga tggagcccgg
     1261 gtggagttcc gatgtgaccc cgacttccat ctggtgggca gctcccggag cgtctgtagt
     1321 cagggccagt ggagcacccc caagccccac tgccaggtga atcgaacgcc acactcagaa
     1381 cggcgtgcag tatacatcgg ggcgctgttt cccatgagcg ggggctggcc ggggggccag
     1441 gcctgccagc ccgcggtgga gatggcgctg gaggacgtta acagccgcag agacatcctg
     1501 ccggactacg agctcaagct tatccaccac gacagcaagt gtgacccagg gcaagccacc
     1561 aagtacttgt acgaactact ctacaatgac cccatcaaga tcattctcat gcctggctgt
     1621 agttctgtct ccacacttgt agctgaggct gcccggatgt ggaaccttat tgtgctctca
     1681 tatggctcca gttcaccagc cttgtcaaac cgacagcggt ttcccacgtt cttccggacg
     1741 catccatccg ccacactcca caatcccacc cgggtgaaac tcttcgaaaa gtggggctgg
     1801 aagaagatcg ctaccatcca acagaccacc gaggtcttca cctcaacgct ggatgacctg
     1861 gaggagcgag tgaaagaggc tgggatcgag atcactttcc gacagagttt cttctcggat
     1921 ccagctgtgc ctgttaaaaa cctgaagcgt caagatgctc gaatcatcgt gggacttttc
     1981 tatgagacgg aagcccggaa agttttttgt gaggtctata aggaaaggct ctttgggaag
     2041 aagtacgtct ggttcctcat cgggtggtat gctgacaact ggttcaagac ctatgacccg
     2101 tcaatcaatt gtacagtgga agaaatgacc gaggcggtgg agggccacat caccacggag
     2161 attgtcatgc tgaaccctgc caacacccga agcatttcca acatgacgtc acaggaattt
     2221 gtggagaaac taaccaagcg gctgaaaaga caccccgagg agactggagg cttccaggag
     2281 gcaccactgg cctatgatgc tatctgggcc ttggctttgg ccttgaacaa gacgtctgga
     2341 ggaggtggtc gttccggcgt gcgcctggag gactttaact acaacaacca gaccattaca
     2401 gaccagatct accgggccat gaactcctcc tcctttgagg gcgtttctgg ccatgtggtc
     2461 tttgatgcca gcggctcccg gatggcatgg acacttatcg agcagctaca gggcggcagc
     2521 tacaagaaga tcggctacta cgacagcacc aaggatgatc tttcctggtc caaaacggac
     2581 aagtggattg gagggtctcc cccagctgac cagaccttgg tcatcaagac attccgtttc
     2641 ctgtctcaga aactctttgg aggcagcggt ggaaccatgt ctaaaggtga agaattattc
     2701 actggtgttg tcccaatttt ggttgaatta gatggtgatg ttaatggtca caaattttct
     2761 gtctccggtg aaggtgaagg tgatgctacg tacggtaaat tgaccttaaa attactctgt
     2821 actactggta aattgccagt tccatggcca accttagtca ctactttagg ttatggtgtt
     2881 caatgttttg ctagataccc agatcatatg aaacaacatg actttttcaa gtctgccatg
     2941 ccagaaggtt atgttcaaga aagaactatt tttttcaaag atgacggtaa ctacaagacc
     3001 agagctgaag tcaagtttga aggtgatacc ttagttaata gaatcgaatt aaaaggtatt
     3061 gattttaaag aagatggtaa cattttaggt cacaaattgg aatacaacta taactctcac
     3121 aatgtttaca tcactgctga caaacaaaag aatggtatca aagctaactt caaaattaga
     3181 cacaacattg aagatggtgg tgttcaatta gctgaccatt atcaacaaaa tactccaatt
     3241 ggtgatggtc cagtcttgtt accagacaac cattacttat cctatcaatc tgccttattc
     3301 aaagatccaa acgaaaagag agaccacatg gtcttgttag aatttttgac tgctgctggt
     3361 attaccgagg gtatgaatga attgtacaaa tgagaattcg aagcttgatc cggctgctaa
     3421 caaagcccga aaggaagctg agttggctgc tgccaccgct gagcaataac tagcataacc
     3481 ccttggggcc tctaaacggg tcttgagggg ttttttgctg aaaggaggaa ctatatccgg
     3541 atctggcgta atagcgaaga ggcccgcacc gatcgccctt cccaacagtt gcgcagcctg
     3601 aatggcgaat gggacgcgcc ctgtagcggc gcattaagcg cggcgggtgt ggtggttacg
     3661 cgcagcgtga ccgctacact tgccagcgcc ctagcgcccg ctcctttcgc tttcttccct
     3721 tcctttctcg ccacgttcgc cggctttccc cgtcaagctc taaatcgggg gctcccttta
     3781 gggttccgat ttagtgcttt acggcacctc gaccccaaaa aacttgatta gggtgatggt
     3841 tcacgtagtg ggccatcgcc ctgatagacg gtttttcgcc ctttgacgtt ggagtccacg
     3901 ttctttaata gtggactctt gttccaaact ggaacaacac tcaaccctat ctcggtctat
     3961 tcttttgatt tataagggat tttgccgatt tcggcctatt ggttaaaaaa tgagctgatt
     4021 taacaaaaat ttaacgcgaa ttttaacaaa atattaacgc ttacaattta ggtggcactt
     4081 ttcggggaaa tgtgcgcgga acccctattt gtttattttt ctaaatacat tcaaatatgt
     4141 atccgctcat gagacaataa ccctgataaa tgcttcaata atattgaaaa aggaagagta
     4201 tgagtattca acatttccgt gtcgccctta ttcccttttt tgcggcattt tgccttcctg
     4261 tttttgctca cccagaaacg ctggtgaaag taaaagatgc tgaagatcag ttgggtgcac
     4321 gagtgggtta catcgaactg gatctcaaca gcggtaagat ccttgagagt tttcgccccg
     4381 aagaacgttt tccaatgatg agcactttta aagttctgct atgtggcgcg gtattatccc
     4441 gtattgacgc cgggcaagag caactcggtc gccgcataca ctattctcag aatgacttgg
     4501 ttgagtactc accagtcaca gaaaagcatc ttacggatgg catgacagta agagaattat
     4561 gcagtgctgc cataaccatg agtgataaca ctgcggccaa cttacttctg acaacgatcg
     4621 gaggaccgaa ggagctaacc gcttttttgc acaacatggg ggatcatgta actcgccttg
     4681 atcgttggga accggagctg aatgaagcca taccaaacga cgagcgtgac accacgatgc
     4741 ctgtagcaat ggcaacaacg ttgcgcaaac tattaactgg cgaactactt actctagctt
     4801 cccggcaaca attaatagac tggatggagg cggataaagt tgcaggacca cttctgcgct
     4861 cggcccttcc ggctggctgg tttattgctg ataaatctgg agccggtgag cgtgggtctc
     4921 gcggtatcat tgcagcactg gggccagatg gtaagccctc ccgtatcgta gttatctaca
     4981 cgacggggag tcaggcaact atggatgaac gaaatagaca gatcgctgag ataggtgcct
     5041 cactgattaa gcattggtaa ctgtcagacc aagtttactc atatatactt tagattgatt
     5101 taaaacttca tttttaattt aaaaggatct aggtgaagat cctttttgat aatctcatga
     5161 ccaaaatccc ttaacgtgag ttttcgttcc actgagcgtc agaccccgta gaaaagatca
     5221 aaggatcttc ttgagatcct ttttttctgc gcgtaatctg ctgcttgcaa acaaaaaaac
     5281 caccgctacc agcggtggtt tgtttgccgg atcaagagct accaactctt tttccgaagg
     5341 taactggctt cagcagagcg cagataccaa atactgttct tctagtgtag ccgtagttag
     5401 gccaccactt caagaactct gtagcaccgc ctacatacct cgctctgcta atcctgttac
     5461 cagtggctgc tgccagtggc gataagtcgt gtcttaccgg gttggactca agacgatagt
     5521 taccggataa ggcgcagcgg tcgggctgaa cggggggttc gtgcacacag cccagcttgg
     5581 agcgaacgac ctacaccgaa ctgagatacc tacagcgtga gctatgagaa agcgccacgc
     5641 ttcccgaagg gagaaaggcg gacaggtatc cggtaagcgg cagggtcgga acaggagagc
     5701 gcacgaggga gcttccaggg ggaaacgcct ggtatcttta tagtcctgtc gggtttcgcc
     5761 acctctgact tgagcgtcga tttttgtgat gctcgtcagg ggggcggagc ctatggaaaa
     5821 acgccagcaa cgcggccttt ttacggttcc tggccttttg ctggcctttt gctcacatgt
     5881 tctttcctgc gttatcccct gattctgtgg ataaccgtat taccgccttt gagtgagctg
     5941 ataccgctcg ccgcagccga acgaccgagc gcagcgagtc agtgagcgag gaagcggaag
     6001 agcgcccaat acgcaaaccg cctctccccg cgcgttggcc gattcattaa tgcag
//
