LOCUS       pRSET_B.        2887 bp DNA     circular SYN 01-JAN-1980
DEFINITION  Bacterial vector for high-level expression of proteins with a 
            cleavable 6xHis tag. For other reading frames, use pRSET A or pRSET 
            C.
ACCESSION   .
VERSION     .
KEYWORDS    pRSET B
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 2887)
  AUTHORS   Invitrogen (Life Technologies)
  TITLE     Direct Submission
REFERENCE   2  (bases 1 to 2887)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     SGRef: number: 1; type: "Journal Article"
FEATURES             Location/Qualifiers
     source          1..2887
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        20..38
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     RBS             70..92
                     /label=RBS
                     /note="efficient ribosome binding site from bacteriophage
                     T7 gene 10 (Olins and Rangwala, 1989)"
     CDS             100..102
                     /codon_start=1
                     /product="start codon"
                     /label=start codon
                     /note="ATG"
                     /translation="M"
     CDS             112..129
                     /label=6xHis
                     /note="6xHis affinity tag"
     CDS             133..165
                     /label=T7 tag (gene 10 leader)
                     /note="leader peptide from bacteriophage T7 gene 10"
     CDS             169..192
                     /label=Xpress(TM) tag
                     /note="Xpress(TM) epitope tag, including an enterokinase 
                     recognition and cleavage site"
     misc_feature    192..238
                     /label=MCS
                     /note="MCS"
                     /note="multiple cloning site"
     terminator      302..349
                     /label=T7 terminator
                     /note="transcription terminator for bacteriophage T7 RNA 
                     polymerase"
     rep_origin      446..901
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow
                     indicates direction of (+) strand synthesis"
     promoter        927..1031
                     /label=AmpR promoter
     CDS             1032..1889
                     /label=AmpR
                     /note="beta-lactamase"
     rep_origin      2063..2651
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
ORIGIN
        1 gatctcgatc ccgcgaaatt aatacgactc actataggga gaccacaacg gtttccctct
       61 agaaataatt ttgtttaact ttaagaagga gatatacata tgcggggttc tcatcatcat
      121 catcatcatg gtatggctag catgactggt ggacagcaaa tgggtcggga tctgtacgac
      181 gatgacgata aggatccgag ctcgagatct gcagctggta ccatggaatt cgaagcttga
      241 tccggctgct aacaaagccc gaaaggaagc tgagttggct gctgccaccg ctgagcaata
      301 actagcataa ccccttgggg cctctaaacg ggtcttgagg ggttttttgc tgaaaggagg
      361 aactatatcc ggatctggcg taatagcgaa gaggcccgca ccgatcgccc ttcccaacag
      421 ttgcgcagcc tgaatggcga atgggacgcg ccctgtagcg gcgcattaag cgcggcgggt
      481 gtggtggtta cgcgcagcgt gaccgctaca cttgccagcg ccctagcgcc cgctcctttc
      541 gctttcttcc cttcctttct cgccacgttc gccggctttc cccgtcaagc tctaaatcgg
      601 gggctccctt tagggttccg atttagtgct ttacggcacc tcgaccccaa aaaacttgat
      661 tagggtgatg gttcacgtag tgggccatcg ccctgataga cggtttttcg ccctttgacg
      721 ttggagtcca cgttctttaa tagtggactc ttgttccaaa ctggaacaac actcaaccct
      781 atctcggtct attcttttga tttataaggg attttgccga tttcggccta ttggttaaaa
      841 aatgagctga tttaacaaaa atttaacgcg aattttaaca aaatattaac gcttacaatt
      901 taggtggcac ttttcgggga aatgtgcgcg gaacccctat ttgtttattt ttctaaatac
      961 attcaaatat gtatccgctc atgagacaat aaccctgata aatgcttcaa taatattgaa
     1021 aaaggaagag tatgagtatt caacatttcc gtgtcgccct tattcccttt tttgcggcat
     1081 tttgccttcc tgtttttgct cacccagaaa cgctggtgaa agtaaaagat gctgaagatc
     1141 agttgggtgc acgagtgggt tacatcgaac tggatctcaa cagcggtaag atccttgaga
     1201 gttttcgccc cgaagaacgt tttccaatga tgagcacttt taaagttctg ctatgtggcg
     1261 cggtattatc ccgtattgac gccgggcaag agcaactcgg tcgccgcata cactattctc
     1321 agaatgactt ggttgagtac tcaccagtca cagaaaagca tcttacggat ggcatgacag
     1381 taagagaatt atgcagtgct gccataacca tgagtgataa cactgcggcc aacttacttc
     1441 tgacaacgat cggaggaccg aaggagctaa ccgctttttt gcacaacatg ggggatcatg
     1501 taactcgcct tgatcgttgg gaaccggagc tgaatgaagc cataccaaac gacgagcgtg
     1561 acaccacgat gcctgtagca atggcaacaa cgttgcgcaa actattaact ggcgaactac
     1621 ttactctagc ttcccggcaa caattaatag actggatgga ggcggataaa gttgcaggac
     1681 cacttctgcg ctcggccctt ccggctggct ggtttattgc tgataaatct ggagccggtg
     1741 agcgtgggtc tcgcggtatc attgcagcac tggggccaga tggtaagccc tcccgtatcg
     1801 tagttatcta cacgacgggg agtcaggcaa ctatggatga acgaaataga cagatcgctg
     1861 agataggtgc ctcactgatt aagcattggt aactgtcaga ccaagtttac tcatatatac
     1921 tttagattga tttaaaactt catttttaat ttaaaaggat ctaggtgaag atcctttttg
     1981 ataatctcat gaccaaaatc ccttaacgtg agttttcgtt ccactgagcg tcagaccccg
     2041 tagaaaagat caaaggatct tcttgagatc ctttttttct gcgcgtaatc tgctgcttgc
     2101 aaacaaaaaa accaccgcta ccagcggtgg tttgtttgcc ggatcaagag ctaccaactc
     2161 tttttccgaa ggtaactggc ttcagcagag cgcagatacc aaatactgtt cttctagtgt
     2221 agccgtagtt aggccaccac ttcaagaact ctgtagcacc gcctacatac ctcgctctgc
     2281 taatcctgtt accagtggct gctgccagtg gcgataagtc gtgtcttacc gggttggact
     2341 caagacgata gttaccggat aaggcgcagc ggtcgggctg aacggggggt tcgtgcacac
     2401 agcccagctt ggagcgaacg acctacaccg aactgagata cctacagcgt gagctatgag
     2461 aaagcgccac gcttcccgaa gggagaaagg cggacaggta tccggtaagc ggcagggtcg
     2521 gaacaggaga gcgcacgagg gagcttccag ggggaaacgc ctggtatctt tatagtcctg
     2581 tcgggtttcg ccacctctga cttgagcgtc gatttttgtg atgctcgtca ggggggcgga
     2641 gcctatggaa aaacgccagc aacgcggcct ttttacggtt cctggccttt tgctggcctt
     2701 ttgctcacat gttctttcct gcgttatccc ctgattctgt ggataaccgt attaccgcct
     2761 ttgagtgagc tgataccgct cgccgcagcc gaacgaccga gcgcagcgag tcagtgagcg
     2821 aggaagcgga agagcgccca atacgcaaac cgcctctccc cgcgcgttgg ccgattcatt
     2881 aatgcag
//
