LOCUS       pRSET_A.        2897 bp DNA     circular SYN 01-JAN-1980
DEFINITION  Bacterial vector for high-level expression of proteins with a 
            cleavable 6xHis tag. For other reading frames, use pRSET B or pRSET 
            C.
ACCESSION   .
VERSION     .
KEYWORDS    pRSET A
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 2897)
  AUTHORS   Invitrogen (Life Technologies)
  TITLE     Direct Submission
REFERENCE   2  (bases 1 to 2897)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     SGRef: number: 1; type: "Journal Article"
FEATURES             Location/Qualifiers
     source          1..2897
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        20..38
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     RBS             70..92
                     /label=RBS
                     /note="efficient ribosome binding site from bacteriophage
                     T7 gene 10 (Olins and Rangwala, 1989)"
     CDS             100..102
                     /codon_start=1
                     /product="start codon"
                     /label=start codon
                     /note="ATG"
                     /translation="M"
     CDS             112..129
                     /label=6xHis
                     /note="6xHis affinity tag"
     CDS             133..165
                     /label=T7 tag (gene 10 leader)
                     /note="leader peptide from bacteriophage T7 gene 10"
     CDS             169..192
                     /label=Xpress(TM) tag
                     /note="Xpress(TM) epitope tag, including an enterokinase 
                     recognition and cleavage site"
     misc_feature    202..248
                     /label=MCS
                     /note="MCS"
                     /note="multiple cloning site"
     terminator      312..359
                     /label=T7 terminator
                     /note="transcription terminator for bacteriophage T7 RNA 
                     polymerase"
     rep_origin      456..911
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow
                     indicates direction of (+) strand synthesis"
     promoter        937..1041
                     /label=AmpR promoter
     CDS             1042..1899
                     /label=AmpR
                     /note="beta-lactamase"
     rep_origin      2073..2661
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
ORIGIN
        1 gatctcgatc ccgcgaaatt aatacgactc actataggga gaccacaacg gtttccctct
       61 agaaataatt ttgtttaact ttaagaagga gatatacata tgcggggttc tcatcatcat
      121 catcatcatg gtatggctag catgactggt ggacagcaaa tgggtcggga tctgtacgac
      181 gatgacgata aggatcgatg gggatccgag ctcgagatct gcagctggta ccatggaatt
      241 cgaagcttga tccggctgct aacaaagccc gaaaggaagc tgagttggct gctgccaccg
      301 ctgagcaata actagcataa ccccttgggg cctctaaacg ggtcttgagg ggttttttgc
      361 tgaaaggagg aactatatcc ggatctggcg taatagcgaa gaggcccgca ccgatcgccc
      421 ttcccaacag ttgcgcagcc tgaatggcga atgggacgcg ccctgtagcg gcgcattaag
      481 cgcggcgggt gtggtggtta cgcgcagcgt gaccgctaca cttgccagcg ccctagcgcc
      541 cgctcctttc gctttcttcc cttcctttct cgccacgttc gccggctttc cccgtcaagc
      601 tctaaatcgg gggctccctt tagggttccg atttagtgct ttacggcacc tcgaccccaa
      661 aaaacttgat tagggtgatg gttcacgtag tgggccatcg ccctgataga cggtttttcg
      721 ccctttgacg ttggagtcca cgttctttaa tagtggactc ttgttccaaa ctggaacaac
      781 actcaaccct atctcggtct attcttttga tttataaggg attttgccga tttcggccta
      841 ttggttaaaa aatgagctga tttaacaaaa atttaacgcg aattttaaca aaatattaac
      901 gcttacaatt taggtggcac ttttcgggga aatgtgcgcg gaacccctat ttgtttattt
      961 ttctaaatac attcaaatat gtatccgctc atgagacaat aaccctgata aatgcttcaa
     1021 taatattgaa aaaggaagag tatgagtatt caacatttcc gtgtcgccct tattcccttt
     1081 tttgcggcat tttgccttcc tgtttttgct cacccagaaa cgctggtgaa agtaaaagat
     1141 gctgaagatc agttgggtgc acgagtgggt tacatcgaac tggatctcaa cagcggtaag
     1201 atccttgaga gttttcgccc cgaagaacgt tttccaatga tgagcacttt taaagttctg
     1261 ctatgtggcg cggtattatc ccgtattgac gccgggcaag agcaactcgg tcgccgcata
     1321 cactattctc agaatgactt ggttgagtac tcaccagtca cagaaaagca tcttacggat
     1381 ggcatgacag taagagaatt atgcagtgct gccataacca tgagtgataa cactgcggcc
     1441 aacttacttc tgacaacgat cggaggaccg aaggagctaa ccgctttttt gcacaacatg
     1501 ggggatcatg taactcgcct tgatcgttgg gaaccggagc tgaatgaagc cataccaaac
     1561 gacgagcgtg acaccacgat gcctgtagca atggcaacaa cgttgcgcaa actattaact
     1621 ggcgaactac ttactctagc ttcccggcaa caattaatag actggatgga ggcggataaa
     1681 gttgcaggac cacttctgcg ctcggccctt ccggctggct ggtttattgc tgataaatct
     1741 ggagccggtg agcgtgggtc tcgcggtatc attgcagcac tggggccaga tggtaagccc
     1801 tcccgtatcg tagttatcta cacgacgggg agtcaggcaa ctatggatga acgaaataga
     1861 cagatcgctg agataggtgc ctcactgatt aagcattggt aactgtcaga ccaagtttac
     1921 tcatatatac tttagattga tttaaaactt catttttaat ttaaaaggat ctaggtgaag
     1981 atcctttttg ataatctcat gaccaaaatc ccttaacgtg agttttcgtt ccactgagcg
     2041 tcagaccccg tagaaaagat caaaggatct tcttgagatc ctttttttct gcgcgtaatc
     2101 tgctgcttgc aaacaaaaaa accaccgcta ccagcggtgg tttgtttgcc ggatcaagag
     2161 ctaccaactc tttttccgaa ggtaactggc ttcagcagag cgcagatacc aaatactgtt
     2221 cttctagtgt agccgtagtt aggccaccac ttcaagaact ctgtagcacc gcctacatac
     2281 ctcgctctgc taatcctgtt accagtggct gctgccagtg gcgataagtc gtgtcttacc
     2341 gggttggact caagacgata gttaccggat aaggcgcagc ggtcgggctg aacggggggt
     2401 tcgtgcacac agcccagctt ggagcgaacg acctacaccg aactgagata cctacagcgt
     2461 gagctatgag aaagcgccac gcttcccgaa gggagaaagg cggacaggta tccggtaagc
     2521 ggcagggtcg gaacaggaga gcgcacgagg gagcttccag ggggaaacgc ctggtatctt
     2581 tatagtcctg tcgggtttcg ccacctctga cttgagcgtc gatttttgtg atgctcgtca
     2641 ggggggcgga gcctatggaa aaacgccagc aacgcggcct ttttacggtt cctggccttt
     2701 tgctggcctt ttgctcacat gttctttcct gcgttatccc ctgattctgt ggataaccgt
     2761 attaccgcct ttgagtgagc tgataccgct cgccgcagcc gaacgaccga gcgcagcgag
     2821 tcagtgagcg aggaagcgga agagcgccca atacgcaaac cgcctctccc cgcgcgttgg
     2881 ccgattcatt aatgcag
//
