LOCUS       V003585                 8182 bp    DNA     circular SYN 18-DEC-2018
DEFINITION  Exported.
ACCESSION   V003585
VERSION     V003585
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
            .
REFERENCE   1  (bases 1 to 8182)
  AUTHORS   Tomlin GC, Wixon JL, Bolotin-Fukuhara M, Oliver SG.
  TITLE     A new family of yeast vectors and S288C-derived strains for the
            systematic analysis of gene function
  JOURNAL   Yeast 18 (6), 563-575 (2001)
   PUBMED   11284012
REFERENCE   2  (bases 1 to 8182)
  AUTHORS   Tomlin GC, Wixon JL, Bolotin-Fukuhara M, Oliver SG.
  TITLE     Direct Submission
  JOURNAL   Submitted (02-AUG-1999) Biological Sciences, University of
            Manchester, 2.205 Stopford Building, Oxford Rd, Manchester M13 9PT,
            United Kingdom
REFERENCE   3  (bases 1 to 8182)
  TITLE     Direct Submission
REFERENCE   4  (bases 1 to 8182)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Yeast";
            date: "2001"; volume: "18"; issue: "6"; pages: "563-575"
            SGRef: number: 2; type: "Journal Article"; journalName: "Submitted
            (02-AUG-1999) Biological Sciences, University of Manchester, 2.205
            Stopford Building, Oxford Rd, Manchester M13 9PT, United Kingdom"
            SGRef: number: 3; type: "Journal Article"
FEATURES             Location/Qualifiers
     source          1..8182
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     CDS             1195..1836
                     /gene="ADE8"
                     /label="Phosphoribosylglycinamide formyltransferase"
                     /note="Phosphoribosylglycinamide formyltransferase from
                     Saccharomyces cerevisiae (strain ATCC 204508 / S288c).
                     Accession#: P04161"
     promoter        2246..2433
                     /label="HIS3 promoter"
     CDS             2434..3093
                     /label="HIS3"
                     /note="imidazoleglycerol-phosphate dehydratase, required
                     for histidine biosynthesis"
     rep_origin      complement(3811..4266)
                     /direction=LEFT
                     /label="f1 ori"
                     /note="f1 bacteriophage origin of replication; arrow
                     indicates direction of (+) strand synthesis"
     primer_bind     4407..4423
                     /label="M13 fwd"
                     /note="common sequencing primer, one of multiple similar
                     variants"
     promoter        4433..4451
                     /label="T7 promoter"
                     /note="promoter for bacteriophage T7 RNA polymerase"
     misc_feature    complement(4460..4567)
                     /label="MCS"
                     /note="pBluescript multiple cloning site"
     promoter        complement(4580..4598)
                     /label="T3 promoter"
                     /note="promoter for bacteriophage T3 RNA polymerase"
     primer_bind     complement(4619..4635)
                     /label="M13 rev"
                     /note="common sequencing primer, one of multiple similar
                     variants"
     protein_bind    complement(4643..4659)
                     /label="lac operator"
                     /note="The lac repressor binds to the lac operator to
                     inhibit transcription in E. coli. This inhibition can be
                     relieved by adding lactose or
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(4667..4697)
                     /label="lac promoter"
                     /note="promoter for the E. coli lac operon"
     protein_bind    complement(4712..4733)
                     /label="CAP binding site"
                     /note="CAP binding activates transcription in the presence
                     of cAMP."
     rep_origin      complement(5021..5609)
                     /direction=LEFT
                     /label="ori"
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
                     replication"
     CDS             complement(5783..6640)
                     /label="AmpR"
                     /note="beta-lactamase"
     promoter        complement(6641..6745)
                     /label="AmpR promoter"
     rep_origin      complement(6772..8114)
                     /direction=LEFT
                     /label="2u ori"
                     /note="yeast 2u plasmid origin of replication"
ORIGIN
        1 tcgcgcgttt cggtgatgac ggtgaaaacc tctgacacat gcagctcccg gagacggtca
       61 cagcttgtct gtaagcggat gccgggagca gacaagcccg tcagggcgcg tcagcgggtg
      121 ttggcgggtg tcggggctgg cttaactatg cggcatcaga gcagattgta ctgagagtgc
      181 accataccac cttttcaatt catcattttt tttttattct tttttttgat ttcggtttct
      241 ttgaaatttt tttgattcgg taatctccga acagaaggaa gaacgaagga aggagcacag
      301 acttagattg gtatatatac gcatggtcga ctctagagga tctttataaa caggcaaagg
      361 ttcattcttt ctgatccgtt ctatcagaaa tttaacggct tgaactctat aaggatcaga
      421 cttgcctact actaaagcgt tctgcaagaa attcctgata aggtcctgta gaacggcctt
      481 gcgaccggaa accgggaaag atacagacct gcaaatatcc ttcaattcgg acactttcag
      541 cagttccatc agagtgatgg tttcctccac ctcgtttctt agttcattag tcggttccct
      601 atcaggccca ggcgtttcat cttcccagta atcctctaaa tttatcattg aaacagaatg
      661 tatatggaac actcaactgt ttgagttgga gtcttctttt cttgtgaaat atggatgaac
      721 tccacttcat tctttcatat tcatattaaa gatgatacac ttttcaactt actatctggt
      781 gcggtgaatg ggtgtataca ttgactacag ctcaggtgac atctaggggc cgtttagtgt
      841 aagaatataa agtacaatcc aaaaaggaaa attatacttg tttaagaaaa gaggaacctt
      901 ctgctcgctc taaaatggta gttctagtga tagtttaaaa atgcattttc cttccgactt
      961 cgaagataga tttcagcata aagactaaaa gcgtcagcaa ggattcgaat gagtgactcg
     1021 ttgctagtga ctctgacctg ttttttattc cagcaagagg aaagttatat agagtagttt
     1081 atgaagagaa gacacttcta aagttgaata acgctcctag cctcaaggtt gatgatttaa
     1141 cttgcagcaa gcgcaggtga gagccaacac acatcaataa tctttccaaa agctatggcc
     1201 agaattgtcg tattaatttc gggctcaggt tccaacttgc aagccttgat cgatgcccaa
     1261 aagcagggcc aattaggcga ggatgctcat attgtctctg tcatatcttc cagcaagaag
     1321 gcatacgggt taactagggc agcggacaat aatatcccca caaaggtttg ttcgctatac
     1381 ccatatacaa agggtattgc gaaggaggac aaagccgcaa gggccaaggc aagatcccag
     1441 tttgagaacg acctcgcaaa actagtgctc gaggaaaagc ctgacgttat catctgcgcg
     1501 ggctggttat tgatccttgg atctactttc ttgagccaac tgcaatctgt gccgatatta
     1561 aatctgcatc cagcgctacc aggttgtttc gatggtacca cacacgcaat tgaaatggca
     1621 tggcgcaaat gtcaggacga gaacaagcct ctgacggccg gatgcatggt gcactatgtc
     1681 atcgaggagg tcgacaaggg tgagccattg gtggtaaaaa agctagaaat catacctggc
     1741 gaagaaacac tagagcagta cgagcaaagg gtacacgatg ctgagcacat cgcgattgta
     1801 gaagctacat ataaggtttt acagcagctt cacaaataag aatagttccc atatttatac
     1861 ttttatattt ggcattcttc aaagtgaggc gcgaaacatc tcgcgtcgta aatcatgatc
     1921 atggattgtg acaaaacgat cttaaaggtt tcgaataaaa tgtgtgtaaa tacgtatact
     1981 tatatatgaa tgtgcatgtg taaacgctta atcgtgttat tgcaaaacta aaatcaggcc
     2041 agattgtagt ttaaacggtt ctagtataac tggtttaagg tgcggctcac aacctgttac
     2101 agggagacaa aaaccatgaa tattcttaag catttcccaa gctatgttgg cccatctaag
     2161 attaggacct tagtgatacc aattggccat tggacgcgaa aagagtttaa taatgcggta
     2221 cagaaacttt ctgaatcggc ctcctctagt acactctata tttttttatg cctcggtaat
     2281 gattttcatt tttttttttc cacctagcgg atgactcttt ttttttctta gcgattggca
     2341 ttatcacata atgaattata cattatataa agtaatgtga tttcttcgaa gaatatacta
     2401 aaaaatgagc aggcaagata aacgaaggca aagatgacag agcagaaagc cctagtaaag
     2461 cgtattacaa atgaaaccaa gattcagatt gcgatctctt taaagggtgg tcccctagcg
     2521 atagagcact cgatcttccc agaaaaagag gcagaagcag tagcagaaca ggccacacaa
     2581 tcgcaagtga ttaacgtcca cacaggtata gggtttctgg accatatgat acatgctctg
     2641 gccaagcatt ccggctggtc gctaatcgtt gagtgcattg gtgacttaca catagacgac
     2701 catcacacca ctgaagactg cgggattgct ctcggtcaag cttttaaaga ggccctaggg
     2761 gccgtgcgtg gagtaaaaag gtttggatca ggatttgcgc ctttggatga ggcactttcc
     2821 agagcggtgg tagatctttc gaacaggccg tacgcagttg tcgaacttgg tttgcaaagg
     2881 gagaaagtag gagatctctc ttgcgagatg atcccgcatt ttcttgaaag ctttgcagag
     2941 gctagcagaa ttaccctcca cgttgattgt ctgcgaggca agaatgatca tcaccgtagt
     3001 gagagtgcgt tcaaggctct tgcggttgcc ataagagaag ccacctcgcc caatggtacc
     3061 aacgatgttc cctccaccaa aggtgttctt atgtagtgac accgattatt taaagctgca
     3121 gcatacgata tatatacatg tgtatatatg tatacctatg aatgtcagta agtatgtata
     3181 cgaacagtat gatactgaag atgacaaggt aatgcatcat tctatacgtg tcattctgaa
     3241 cgaggcgcgc ccatgcaggt attgttagcg gtttgaagca ggcggcagaa gaagtaacaa
     3301 aggaacctag aggccttttg atgttagcag aattgtcatg caagggctcc ctatctactg
     3361 gagaatatac taagggtact gttgacattg cgaagagcga caaagatttt gttatcggct
     3421 ttattgctca aagagacatg ggtggaagag atgaaggtta cgattggttg attatgacac
     3481 ccggtgtggg tttagatgac aagggagacg cattgggtca acagtataga accgtggatg
     3541 atgtggtctc tacaggatct gacattatta ttgttggaag aggactattt gcaaagggaa
     3601 gggatgctaa ggtagagggt gaacgttaca gaaaagcagg ctgggaagca tatttgagaa
     3661 gatgcggcca gcaaaactaa aaaactgtat tataagtaaa tgcatgtata ctaaactcac
     3721 aaattagagc ttcaatttaa ttatatcagt tattacccta tgcggtgtga aataccgcac
     3781 agatgcgtaa ggagaaaata ccgcatcagg aaattgtaaa cgttaatatt ttgttaaaat
     3841 tcgcgttaaa tttttgttaa atcagctcat tttttaacca ataggccgaa atcggcaaaa
     3901 tcccttataa atcaaaagaa tagaccgaga tagggttgag tgttgttcca gtttggaaca
     3961 agagtccact attaaagaac gtggactcca acgtcaaagg gcgaaaaacc gtctatcagg
     4021 gcgatggccc actacgtgaa ccatcaccct aatcaagttt tttggggtcg aggtgccgta
     4081 aagcactaaa tcggaaccct aaagggagcc cccgatttag agcttgacgg ggaaagccgg
     4141 cgaacgtggc gagaaaggaa gggaagaaag cgaaaggagc gggcgctagg gcgctggcaa
     4201 gtgtagcggt cacgctgcgc gtaaccacca cacccgccgc gcttaatgcg ccgctacagg
     4261 gcgcgtccat tcgccattca ggctgcgcaa ctgttgggaa gggcgatcgg tgcgggcctc
     4321 ttcgctatta cgccagctgg cgaaaggggg atgtgctgca aggcgattaa gttgggtaac
     4381 gccagggttt tcccagtcac gacgttgtaa aacgacggcc agtgagcgcg cgtaatacga
     4441 ctcactatag ggcgaattgg gtaccgggcc ccccctcgag gtcgacggta tcgataagct
     4501 tgatatcgaa ttcctgcagc ccgggggatc cactagttct agagcggccg ccaccgcggt
     4561 ggagctccag cttttgttcc ctttagtgag ggttaattgc gcgcttggcg taatcatggt
     4621 catagctgtt tcctgtgtga aattgttatc cgctcacaat tccacacaac ataggagccg
     4681 gaagcataaa gtgtaaagcc tggggtgcct aatgagtgag gtaactcaca ttaattgcgt
     4741 tgcgctcact gcccgctttc cagtcgggaa acctgtcgtg ccagctgcat taatgaatcg
     4801 gccaacgcgc ggggagaggc ggtttgcgta ttgggcgctc ttccgcttcc tcgctcactg
     4861 actcgctgcg ctcggtcgtt cggctgcggc gagcggtatc agctcactca aaggcggtaa
     4921 tacggttatc cacagaatca ggggataacg caggaaagaa catgtgagca aaaggccagc
     4981 aaaaggccag gaaccgtaaa aaggccgcgt tgctggcgtt tttccatagg ctccgccccc
     5041 ctgacgagca tcacaaaaat cgacgctcaa gtcagaggtg gcgaaacccg acaggactat
     5101 aaagatacca ggcgtttccc cctggaagct ccctcgtgcg ctctcctgtt ccgaccctgc
     5161 cgcttaccgg atacctgtcc gcctttctcc cttcgggaag cgtggcgctt tctcatagct
     5221 cacgctgtag gtatctcagt tcggtgtagg tcgttcgctc caagctgggc tgtgtgcacg
     5281 aaccccccgt tcagcccgac cgctgcgcct tatccggtaa ctatcgtctt gagtccaacc
     5341 cggtaagaca cgacttatcg ccactggcag cagccactgg taacaggatt agcagagcga
     5401 ggtatgtagg cggtgctaca gagttcttga agtggtggcc taactacggc tacactagaa
     5461 ggacagtatt tggtatctgc gctctgctga agccagttac cttcggaaaa agagttggta
     5521 gctcttgatc cggcaaacaa accaccgctg gtagcggtgg tttttttgtt tgcaagcagc
     5581 agattacgcg cagaaaaaaa ggatctcaag aagatccttt gatcttttct acggggtctg
     5641 acgctcagtg gaacgaaaac tcacgttaag ggattttggt catgagatta tcaaaaagga
     5701 tcttcaccta gatcctttta aattaaaaat gaagttttaa atcaatctaa agtatatatg
     5761 agtaaacttg gtctgacagt taccaatgct taatcagtga ggcacctatc tcagcgatct
     5821 gtctatttcg ttcatccata gttgcctgac tccccgtcgt gtagataact acgatacggg
     5881 agggcttacc atctggcccc agtgctgcaa tgataccgcg agacccacgc tcaccggctc
     5941 cagatttatc agcaataaac cagccagccg gaagggccga gcgcagaagt ggtcctgcaa
     6001 ctttatccgc ctccatccag tctattaatt gttgccggga agctagagta agtagttcgc
     6061 cagttaatag tttgcgcaac gttgttgcca ttgctacagg catcgtggtg tcacgctcgt
     6121 cgtttggtat ggcttcattc agctccggtt cccaacgatc aaggcgagtt acatgatccc
     6181 ccatgttgtg caaaaaagcg gttagctcct tcggtcctcc gatcgttgtc agaagtaagt
     6241 tggccgcagt gttatcactc atggttatgg cagcactgca taattctctt actgtcatgc
     6301 catccgtaag atgcttttct gtgactggtg agtactcaac caagtcattc tgagaatagt
     6361 gtatgcggcg accgagttgc tcttgcccgg cgtcaatacg ggataatacc gcgccacata
     6421 gcagaacttt aaaagtgctc atcattggaa aacgttcttc ggggcgaaaa ctctcaagga
     6481 tcttaccgct gttgagatcc agttcgatgt aacccactcg tgcacccaac tgatcttcag
     6541 catcttttac tttcaccagc gtttctgggt gagcaaaaac aggaaggcaa aatgccgcaa
     6601 aaaagggaat aagggcgaca cggaaatgtt gaatactcat actcttcctt tttcaatatt
     6661 attgaagcat ttatcagggt tattgtctca tgagcggata catatttgaa tgtatttaga
     6721 aaaataaaca aataggggtt ccgcgcacat ttccccgaaa agtgccacct gaacgaagca
     6781 tctgtgcttc attttgtaga acaaaaatgc aacgcgagag cgctaatttt tcaaacaaag
     6841 aatctgagct gcatttttac agaacagaaa tgcaacgcga aagcgctatt ttaccaacga
     6901 agaatctgtg cttcattttt gtaaaacaaa aatgcaacgc gagagcgcta atttttcaaa
     6961 caaagaatct gagctgcatt tttacagaac agaaatgcaa cgcgagagcg ctattttacc
     7021 aacaaagaat ctatacttct tttttgttct acaaaaatgc atcccgagag cgctattttt
     7081 ctaacaaagc atcttagatt actttttttc tcctttgtgc gctctataat gcagtctctt
     7141 gataactttt tgcactgtag gtccgttaag gttagaagaa ggctactttg gtgtctattt
     7201 tctcttccat aaaaaaagcc tgactccact tcccgcgttt actgattact agcgaagctg
     7261 cgggtgcatt ttttcaagat aaaggcatcc ccgattatat tctataccga tgtggattgc
     7321 gcatactttg tgaacagaaa gtgatagcgt tgatgattct tcattggtca gaaaattatg
     7381 aacggtttct tctattttgt ctctatatac tacgtatagg aaatgtttac attttcgtat
     7441 tgttttcgat tcactctatg aatagttctt actacaattt ttttgtctaa agagtaatac
     7501 tagagataaa cataaaaaat gtagaggtcg agtttagatg caagttcaag gagcgaaagg
     7561 tggatgggta ggttatatag ggatatagca cagagatata tagcaaagag atacttttga
     7621 gcaatgtttg tggaagcggt attcgcaata ttttagtagc tcgttacagt ccggtgcgtt
     7681 tttggttttt tgaaagtgcg tcttcagagc gcttttggtt ttcaaaagcg ctctgaagtt
     7741 cctatacttt ctagagaata ggaacttcgg aataggaact tcaaagcgtt tccgaaaacg
     7801 agcgcttccg aaaatgcaac gcgagctgcg cacatacagc tcactgttca cgtcgcacct
     7861 atatctgcgt gttgcctgta tatatatata catgagaaga acggcatagt gcgtgtttat
     7921 gcttaaatgc gtacttatat gcgtctattt atgtaggatg aaaggtagtc tagtacctcc
     7981 tgtgatatta tcccattcca tgcggggtat cgtatgcttc cttcagcact accctttagc
     8041 tgttctatat gctgccactc ctcaattgga ttagtctcat ccttcaatgc tatcatttcc
     8101 tttgatattg gatcatacta agaaaccatt attatcatga cattaaccta taaaaatagg
     8161 cgtatcacga ggccctttcg tc
//
