LOCUS       V003586                 7168 bp    DNA     circular SYN 18-DEC-2018
DEFINITION  Exported.
ACCESSION   V003586
VERSION     V003586
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
            .
REFERENCE   1  (bases 1 to 7168)
  AUTHORS   Tomlin GC, Wixon JL, Bolotin-Fukuhara M, Oliver SG.
  TITLE     A new family of yeast vectors and S288C-derived strains for the
            systematic analysis of gene function
  JOURNAL   Yeast 18 (6), 563-575 (2001)
   PUBMED   11284012
REFERENCE   2  (bases 1 to 7168)
  AUTHORS   Tomlin GC, Wixon JL, Bolotin-Fukuhara M, Oliver SG.
  TITLE     Direct Submission
  JOURNAL   Submitted (02-AUG-1999) Biological Sciences, University of
            Manchester, 2.205 Stopford Building, Oxford Rd, Manchester M13 9PT,
            United Kingdom
REFERENCE   3  (bases 1 to 7168)
  TITLE     Direct Submission
REFERENCE   4  (bases 1 to 7168)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Yeast";
            date: "2001"; volume: "18"; issue: "6"; pages: "563-575"
            SGRef: number: 2; type: "Journal Article"; journalName: "Submitted
            (02-AUG-1999) Biological Sciences, University of Manchester, 2.205
            Stopford Building, Oxford Rd, Manchester M13 9PT, United Kingdom"
            SGRef: number: 3; type: "Journal Article"
FEATURES             Location/Qualifiers
     source          1..7168
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     CDS             1195..1836
                     /gene="ADE8"
                     /label="Phosphoribosylglycinamide formyltransferase"
                     /note="Phosphoribosylglycinamide formyltransferase from
                     Saccharomyces cerevisiae (strain ATCC 204508 / S288c).
                     Accession#: P04161"
     rep_origin      complement(2797..3252)
                     /direction=LEFT
                     /label="f1 ori"
                     /note="f1 bacteriophage origin of replication; arrow
                     indicates direction of (+) strand synthesis"
     primer_bind     3393..3409
                     /label="M13 fwd"
                     /note="common sequencing primer, one of multiple similar
                     variants"
     promoter        3419..3437
                     /label="T7 promoter"
                     /note="promoter for bacteriophage T7 RNA polymerase"
     misc_feature    complement(3446..3553)
                     /label="MCS"
                     /note="pBluescript multiple cloning site"
     promoter        complement(3566..3584)
                     /label="T3 promoter"
                     /note="promoter for bacteriophage T3 RNA polymerase"
     primer_bind     complement(3605..3621)
                     /label="M13 rev"
                     /note="common sequencing primer, one of multiple similar
                     variants"
     protein_bind    complement(3629..3645)
                     /label="lac operator"
                     /note="The lac repressor binds to the lac operator to
                     inhibit transcription in E. coli. This inhibition can be
                     relieved by adding lactose or
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(3653..3683)
                     /label="lac promoter"
                     /note="promoter for the E. coli lac operon"
     protein_bind    complement(3698..3719)
                     /label="CAP binding site"
                     /note="CAP binding activates transcription in the presence
                     of cAMP."
     rep_origin      complement(4007..4595)
                     /direction=LEFT
                     /label="ori"
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
                     replication"
     CDS             complement(4769..5626)
                     /label="AmpR"
                     /note="beta-lactamase"
     promoter        complement(5627..5731)
                     /label="AmpR promoter"
     rep_origin      complement(5758..7100)
                     /direction=LEFT
                     /label="2u ori"
                     /note="yeast 2u plasmid origin of replication"
ORIGIN
        1 tcgcgcgttt cggtgatgac ggtgaaaacc tctgacacat gcagctcccg gagacggtca
       61 cagcttgtct gtaagcggat gccgggagca gacaagcccg tcagggcgcg tcagcgggtg
      121 ttggcgggtg tcggggctgg cttaactatg cggcatcaga gcagattgta ctgagagtgc
      181 accataccac cttttcaatt catcattttt tttttattct tttttttgat ttcggtttct
      241 ttgaaatttt tttgattcgg taatctccga acagaaggaa gaacgaagga aggagcacag
      301 acttagattg gtatatatac gcatggtcga ctctagagga tctttataaa caggcaaagg
      361 ttcattcttt ctgatccgtt ctatcagaaa tttaacggct tgaactctat aaggatcaga
      421 cttgcctact actaaagcgt tctgcaagaa attcctgata aggtcctgta gaacggcctt
      481 gcgaccggaa accgggaaag atacagacct gcaaatatcc ttcaattcgg acactttcag
      541 cagttccatc agagtgatgg tttcctccac ctcgtttctt agttcattag tcggttccct
      601 atcaggccca ggcgtttcat cttcccagta atcctctaaa tttatcattg aaacagaatg
      661 tatatggaac actcaactgt ttgagttgga gtcttctttt cttgtgaaat atggatgaac
      721 tccacttcat tctttcatat tcatattaaa gatgatacac ttttcaactt actatctggt
      781 gcggtgaatg ggtgtataca ttgactacag ctcaggtgac atctaggggc cgtttagtgt
      841 aagaatataa agtacaatcc aaaaaggaaa attatacttg tttaagaaaa gaggaacctt
      901 ctgctcgctc taaaatggta gttctagtga tagtttaaaa atgcattttc cttccgactt
      961 cgaagataga tttcagcata aagactaaaa gcgtcagcaa ggattcgaat gagtgactcg
     1021 ttgctagtga ctctgacctg ttttttattc cagcaagagg aaagttatat agagtagttt
     1081 atgaagagaa gacacttcta aagttgaata acgctcctag cctcaaggtt gatgatttaa
     1141 cttgcagcaa gcgcaggtga gagccaacac acatcaataa tctttccaaa agctatggcc
     1201 agaattgtcg tattaatttc gggctcaggt tccaacttgc aagccttgat cgatgcccaa
     1261 aagcagggcc aattaggcga ggatgctcat attgtctctg tcatatcttc cagcaagaag
     1321 gcatacgggt taactagggc agcggacaat aatatcccca caaaggtttg ttcgctatac
     1381 ccatatacaa agggtattgc gaaggaggac aaagccgcaa gggccaaggc aagatcccag
     1441 tttgagaacg acctcgcaaa actagtgctc gaggaaaagc ctgacgttat catctgcgcg
     1501 ggctggttat tgatccttgg atctactttc ttgagccaac tgcaatctgt gccgatatta
     1561 aatctgcatc cagcgctacc aggttgtttc gatggtacca cacacgcaat tgaaatggca
     1621 tggcgcaaat gtcaggacga gaacaagcct ctgacggccg gatgcatggt gcactatgtc
     1681 atcgaggagg tcgacaaggg tgagccattg gtggtaaaaa agctagaaat catacctggc
     1741 gaagaaacac tagagcagta cgagcaaagg gtacacgatg ctgagcacat cgcgattgta
     1801 gaagctacat ataaggtttt acagcagctt cacaaataag aatagttccc atatttatac
     1861 ttttatattt ggcattcttc aaagtgaggc gcgaaacatc tcgcgtcgta aatcatgatc
     1921 atggattgtg acaaaacgat cttaaaggtt tcgaataaaa tgtgtgtaaa tacgtatact
     1981 tatatatgaa tgtgcatgtg taaacgctta atcgtgttat tgcaaaacta aaatcaggcc
     2041 agattgtagt ttaaacggtt ctagtataac tggtttaagg tgcggctcac aacctgttac
     2101 agggagacaa aaaccatgaa tattcttaag catttcccaa gctatgttgg cccatctaag
     2161 attaggacct tagtgatacc aattggccat tggacgcgaa aagagtttaa taatgcggta
     2221 cagaaacttt ctgaattaat tcaggtattg ttagcggttt gaagcaggcg gcagaagaag
     2281 taacaaagga acctagaggc cttttgatgt tagcagaatt gtcatgcaag ggctccctat
     2341 ctactggaga atatactaag ggtactgttg acattgcgaa gagcgacaaa gattttgtta
     2401 tcggctttat tgctcaaaga gacatgggtg gaagagatga aggttacgat tggttgatta
     2461 tgacacccgg tgtgggttta gatgacaagg gagacgcatt gggtcaacag tatagaaccg
     2521 tggatgatgt ggtctctaca ggatctgaca ttattattgt tggaagagga ctatttgcaa
     2581 agggaaggga tgctaaggta gagggtgaac gttacagaaa agcaggctgg gaagcatatt
     2641 tgagaagatg cggccagcaa aactaaaaaa ctgtattata agtaaatgca tgtatactaa
     2701 actcacaaat tagagcttca atttaattat atcagttatt accctatgcg gtgtgaaata
     2761 ccgcacagat gcgtaaggag aaaataccgc atcaggaaat tgtaaacgtt aatattttgt
     2821 taaaattcgc gttaaatttt tgttaaatca gctcattttt taaccaatag gccgaaatcg
     2881 gcaaaatccc ttataaatca aaagaataga ccgagatagg gttgagtgtt gttccagttt
     2941 ggaacaagag tccactatta aagaacgtgg actccaacgt caaagggcga aaaaccgtct
     3001 atcagggcga tggcccacta cgtgaaccat caccctaatc aagttttttg gggtcgaggt
     3061 gccgtaaagc actaaatcgg aaccctaaag ggagcccccg atttagagct tgacggggaa
     3121 agccggcgaa cgtggcgaga aaggaaggga agaaagcgaa aggagcgggc gctagggcgc
     3181 tggcaagtgt agcggtcacg ctgcgcgtaa ccaccacacc cgccgcgctt aatgcgccgc
     3241 tacagggcgc gtccattcgc cattcaggct gcgcaactgt tgggaagggc gatcggtgcg
     3301 ggcctcttcg ctattacgcc agctggcgaa agggggatgt gctgcaaggc gattaagttg
     3361 ggtaacgcca gggttttccc agtcacgacg ttgtaaaacg acggccagtg agcgcgcgta
     3421 atacgactca ctatagggcg aattgggtac cgggcccccc ctcgaggtcg acggtatcga
     3481 taagcttgat atcgaattcc tgcagcccgg gggatccact agttctagag cggccgccac
     3541 cgcggtggag ctccagcttt tgttcccttt agtgagggtt aattgcgcgc ttggcgtaat
     3601 catggtcata gctgtttcct gtgtgaaatt gttatccgct cacaattcca cacaacatag
     3661 gagccggaag cataaagtgt aaagcctggg gtgcctaatg agtgaggtaa ctcacattaa
     3721 ttgcgttgcg ctcactgccc gctttccagt cgggaaacct gtcgtgccag ctgcattaat
     3781 gaatcggcca acgcgcgggg agaggcggtt tgcgtattgg gcgctcttcc gcttcctcgc
     3841 tcactgactc gctgcgctcg gtcgttcggc tgcggcgagc ggtatcagct cactcaaagg
     3901 cggtaatacg gttatccaca gaatcagggg ataacgcagg aaagaacatg tgagcaaaag
     3961 gccagcaaaa ggccaggaac cgtaaaaagg ccgcgttgct ggcgtttttc cataggctcc
     4021 gcccccctga cgagcatcac aaaaatcgac gctcaagtca gaggtggcga aacccgacag
     4081 gactataaag ataccaggcg tttccccctg gaagctccct cgtgcgctct cctgttccga
     4141 ccctgccgct taccggatac ctgtccgcct ttctcccttc gggaagcgtg gcgctttctc
     4201 atagctcacg ctgtaggtat ctcagttcgg tgtaggtcgt tcgctccaag ctgggctgtg
     4261 tgcacgaacc ccccgttcag cccgaccgct gcgccttatc cggtaactat cgtcttgagt
     4321 ccaacccggt aagacacgac ttatcgccac tggcagcagc cactggtaac aggattagca
     4381 gagcgaggta tgtaggcggt gctacagagt tcttgaagtg gtggcctaac tacggctaca
     4441 ctagaaggac agtatttggt atctgcgctc tgctgaagcc agttaccttc ggaaaaagag
     4501 ttggtagctc ttgatccggc aaacaaacca ccgctggtag cggtggtttt tttgtttgca
     4561 agcagcagat tacgcgcaga aaaaaaggat ctcaagaaga tcctttgatc ttttctacgg
     4621 ggtctgacgc tcagtggaac gaaaactcac gttaagggat tttggtcatg agattatcaa
     4681 aaaggatctt cacctagatc cttttaaatt aaaaatgaag ttttaaatca atctaaagta
     4741 tatatgagta aacttggtct gacagttacc aatgcttaat cagtgaggca cctatctcag
     4801 cgatctgtct atttcgttca tccatagttg cctgactccc cgtcgtgtag ataactacga
     4861 tacgggaggg cttaccatct ggccccagtg ctgcaatgat accgcgagac ccacgctcac
     4921 cggctccaga tttatcagca ataaaccagc cagccggaag ggccgagcgc agaagtggtc
     4981 ctgcaacttt atccgcctcc atccagtcta ttaattgttg ccgggaagct agagtaagta
     5041 gttcgccagt taatagtttg cgcaacgttg ttgccattgc tacaggcatc gtggtgtcac
     5101 gctcgtcgtt tggtatggct tcattcagct ccggttccca acgatcaagg cgagttacat
     5161 gatcccccat gttgtgcaaa aaagcggtta gctccttcgg tcctccgatc gttgtcagaa
     5221 gtaagttggc cgcagtgtta tcactcatgg ttatggcagc actgcataat tctcttactg
     5281 tcatgccatc cgtaagatgc ttttctgtga ctggtgagta ctcaaccaag tcattctgag
     5341 aatagtgtat gcggcgaccg agttgctctt gcccggcgtc aatacgggat aataccgcgc
     5401 cacatagcag aactttaaaa gtgctcatca ttggaaaacg ttcttcgggg cgaaaactct
     5461 caaggatctt accgctgttg agatccagtt cgatgtaacc cactcgtgca cccaactgat
     5521 cttcagcatc ttttactttc accagcgttt ctgggtgagc aaaaacagga aggcaaaatg
     5581 ccgcaaaaaa gggaataagg gcgacacgga aatgttgaat actcatactc ttcctttttc
     5641 aatattattg aagcatttat cagggttatt gtctcatgag cggatacata tttgaatgta
     5701 tttagaaaaa taaacaaata ggggttccgc gcacatttcc ccgaaaagtg ccacctgaac
     5761 gaagcatctg tgcttcattt tgtagaacaa aaatgcaacg cgagagcgct aatttttcaa
     5821 acaaagaatc tgagctgcat ttttacagaa cagaaatgca acgcgaaagc gctattttac
     5881 caacgaagaa tctgtgcttc atttttgtaa aacaaaaatg caacgcgaga gcgctaattt
     5941 ttcaaacaaa gaatctgagc tgcattttta cagaacagaa atgcaacgcg agagcgctat
     6001 tttaccaaca aagaatctat acttcttttt tgttctacaa aaatgcatcc cgagagcgct
     6061 atttttctaa caaagcatct tagattactt tttttctcct ttgtgcgctc tataatgcag
     6121 tctcttgata actttttgca ctgtaggtcc gttaaggtta gaagaaggct actttggtgt
     6181 ctattttctc ttccataaaa aaagcctgac tccacttccc gcgtttactg attactagcg
     6241 aagctgcggg tgcatttttt caagataaag gcatccccga ttatattcta taccgatgtg
     6301 gattgcgcat actttgtgaa cagaaagtga tagcgttgat gattcttcat tggtcagaaa
     6361 attatgaacg gtttcttcta ttttgtctct atatactacg tataggaaat gtttacattt
     6421 tcgtattgtt ttcgattcac tctatgaata gttcttacta caattttttt gtctaaagag
     6481 taatactaga gataaacata aaaaatgtag aggtcgagtt tagatgcaag ttcaaggagc
     6541 gaaaggtgga tgggtaggtt atatagggat atagcacaga gatatatagc aaagagatac
     6601 ttttgagcaa tgtttgtgga agcggtattc gcaatatttt agtagctcgt tacagtccgg
     6661 tgcgtttttg gttttttgaa agtgcgtctt cagagcgctt ttggttttca aaagcgctct
     6721 gaagttccta tactttctag agaataggaa cttcggaata ggaacttcaa agcgtttccg
     6781 aaaacgagcg cttccgaaaa tgcaacgcga gctgcgcaca tacagctcac tgttcacgtc
     6841 gcacctatat ctgcgtgttg cctgtatata tatatacatg agaagaacgg catagtgcgt
     6901 gtttatgctt aaatgcgtac ttatatgcgt ctatttatgt aggatgaaag gtagtctagt
     6961 acctcctgtg atattatccc attccatgcg gggtatcgta tgcttccttc agcactaccc
     7021 tttagctgtt ctatatgctg ccactcctca attggattag tctcatcctt caatgctatc
     7081 atttcctttg atattggatc atactaagaa accattatta tcatgacatt aacctataaa
     7141 aataggcgta tcacgaggcc ctttcgtc
//
