LOCUS       40924_37923        7500 bp DNA     circular SYN 18-DEC-2018
DEFINITION  Shuttle vector pRS415, complete sequence.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 7500)
  AUTHORS   Pinto MR, Marchini JF, Buranello PA, Oliver C, Mortara RA, Tosi LR.
  TITLE     The Leishmania major HTBF protein belongs in the YIP family of 
            proteins
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 7500)
  AUTHORS   Pinto MR, Marchini JF, Tosi LR.
  TITLE     Direct Submission
  JOURNAL   Submitted (13-AUG-2008) Bilogia Celular e Molecular e Bioagentes 
            Patogenicos, Faculdade de Medicina de Ribeirao Preto, AV. 
            Bandeirantes, 3900, Ribeirao Preto, Sao Paulo 14049-900, Brasil
REFERENCE   3  (bases 1 to 7500)
  TITLE     Direct Submission
REFERENCE   4  (bases 1 to 7500)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: 
            "Unpublished"
COMMENT     SGRef: number: 2; type: "Journal Article"; journalName: "Submitted 
            (13-AUG-2008) Bilogia Celular e Molecular e Bioagentes Patogenicos, 
            Faculdade de Medicina de Ribeirao Preto, AV. Bandeirantes, 3900, 
            Ribeirao Preto, Sao Paulo 14049-900, Brasil"
COMMENT     SGRef: number: 3; type: "Journal Article"
FEATURES             Location/Qualifiers
     source          1..7500
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     CDS             complement(666..1757)
                     /label=LEU2
                     /note="3-isopropylmalate dehydrogenase, required for
                     leucine biosynthesis"
     promoter        complement(1770..2174)
                     /label=LEU2 promoter
     rep_origin      complement(2474..2929)
                     /direction=LEFT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow
                     indicates direction of (+) strand synthesis"
     primer_bind     3074..3090
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     promoter        3100..3118
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     3144..3160
                     /label=KS primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     regulatory      complement(3181..3267)
                     /label=GAL 1
                     /note="GAL 1"
                     /regulatory_class="promoter"
     promoter        complement(3272..3936)
                     /label=GAL1,10 promoter
                     /note="divergent inducible promoter, regulated by Gal4"
     regulatory      3948..4088
                     /label=GAL10
                     /note="GAL10"
                     /regulatory_class="promoter"
     CDS             4103..4672
                     /codon_start=1
                     /product="HTBF"
                     /label=HTBF
                     /note="H region resistance protein; derived from Leishmania
                     terbinafine"
                     /protein_id="ACH69155.1"
                     /translation="MLNEVHVNADPNSISTLDEPVLETLLRDAKAIGRKLVVVVCPSLG
                     GDKELRDWDLWGPLFLCLILASILTINASDDQGAAVFSAVFIFVWLGGLVVTVNAKLLG
                     SKIMFFQTYCAMGYCLAPICLGALLCCVLPWFLLNLMLCFMAWAWACWAALRFFRHTVS
                     ADREVLVVYPVGLFYVFFTWMVLVGI"
     primer_bind     complement(4673..4689)
                     /label=SK primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     promoter        complement(4726..4744)
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     primer_bind     complement(4765..4781)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     protein_bind    complement(4789..4805)
                     /label=lac operator
                     /note="The lac repressor binds to the lac operator to
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(4813..4843)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    complement(4858..4879)
                     /label=CAP binding site
                     /note="CAP binding activates transcription in the presence
                     of cAMP."
     rep_origin      complement(5167..5755)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     CDS             complement(5929..6786)
                     /label=AmpR
                     /note="beta-lactamase"
     promoter        complement(6787..6891)
                     /label=AmpR promoter
     misc_feature    6928..7431
                     /label=CEN/ARS
                     /note="S. cerevisiae CEN6 centromere fused to an
                     autonomously replicating sequence"
ORIGIN
        1 tcgcgcgttt cggtgatgac ggtgaaaacc tctgacacat gcagctcccg gagacggtca
       61 cagcttgtct gtaagcggat gccgggagca gacaagcccg tcagggcgcg tcagcgggtg
      121 ttggcgggtg tcggggctgg cttaactatg cggcatcaga gcagattgta ctgagagtgc
      181 accatatcga ctacgtcgta aggccgtttc tgacagagta aaattcttga gggaactttc
      241 accattatgg gaaatggttc aagaaggtat tgacttaaac tccatcaaat ggtcaggtca
      301 ttgagtgttt tttatttgtt gtattttttt ttttttagag aaaatcctcc aatatcaaat
      361 taggaatcgt agtttcatga ttttctgtta cacctaactt tttgtgtggt gccctcctcc
      421 ttgtcaatat taatgttaaa gtgcaattct ttttccttat cacgttgagc cattagtatc
      481 aatttgctta cctgtattcc tttactatcc tcctttttct ccttcttgat aaatgtatgt
      541 agattgcgta tatagtttcg tctaccctat gaacatattc cattttgtaa tttcgtgtcg
      601 tttctattat gaatttcatt tataaagttt atgtacaaat atcataaaaa aagagaatct
      661 ttttaagcaa ggattttctt aacttcttcg gcgacagcat caccgacttc ggtggtactg
      721 ttggaaccac ctaaatcacc agttctgata cctgcatcca aaaccttttt aactgcatct
      781 tcaatggcct taccttcttc aggcaagttc aatgacaatt tcaacatcat tgcagcagac
      841 aagatagtgg cgatagggtc aaccttattc tttggcaaat ctggagcaga accgtggcat
      901 ggttcgtaca aaccaaatgc ggtgttcttg tctggcaaag aggccaagga cgcagatggc
      961 aacaaaccca aggaacctgg gataacggag gcttcatcgg agatgatatc accaaacatg
     1021 ttgctggtga ttataatacc atttaggtgg gttgggttct taactaggat catggcggca
     1081 gaatcaatca attgatgttg aaccttcaat gtagggaatt cgttcttgat ggtttcctcc
     1141 acagtttttc tccataatct tgaagaggcc aaaacattag ctttatccaa ggaccaaata
     1201 ggcaatggtg gctcatgttg tagggccatg aaagcggcca ttcttgtgat tctttgcact
     1261 tctggaacgg tgtattgttc actatcccaa gcgacaccat caccatcgtc ttcctttctc
     1321 ttaccaaagt aaatacctcc cactaattct ctgacaacaa cgaagtcagt acctttagca
     1381 aattgtggct tgattggaga taagtctaaa agagagtcgg atgcaaagtt acatggtctt
     1441 aagttggcgt acaattgaag ttctttacgg atttttagta aaccttgttc aggtctaaca
     1501 ctaccggtac cccatttagg accacccaca gcacctaaca aaacggcatc aaccttcttg
     1561 gaggcttcca gcgcctcatc tggaagtggg acacctgtag catcgatagc agcaccacca
     1621 attaaatgat tttcgaaatc gaacttgaca ttggaacgaa catcagaaat agctttaaga
     1681 accttaatgg cttcggctgt gatttcttga ccaacgtggt cacctggcaa aacgacgatc
     1741 ttcttagggg cagacatagg ggcagacatt agaatggtat atccttgaaa tatatatata
     1801 tattgctgaa atgtaaaagg taagaaaagt tagaaagtaa gacgattgct aaccacctat
     1861 tggaaaaaac aataggtcct taaataatat tgtcaacttc aagtattgtg atgcaagcat
     1921 ttagtcatga acgcttctct attctatatg aaaagccggt tccggcctct cacctttcct
     1981 ttttctccca atttttcagt tgaaaaaggt atatgcgtca ggcgacctct gaaattaaca
     2041 aaaaatttcc agtcatcgaa tttgattctg tgcgatagcg cccctgtgtg ttctcgttat
     2101 gttgaggaaa aaaataatgg ttgctaagag attcgaactc ttgcatctta cgatacctga
     2161 gtattcccac agttaactgc ggtcaagata tttcttgaat caggcgcctt agaccgctcg
     2221 gccaaacaac caattacttg ttgagaaata gagtataatt atcctataaa tataacgttt
     2281 ttgaacacac atgaacaagg aagtacagga caattgattt tgaagagaat gtggattttg
     2341 atgtaattgt tgggattcca tttttaataa ggcaataata ttaggtatgt ggatatacta
     2401 gaagttctcc tcgaccgtcg atatgcggtg tgaaataccg cacagatgcg taaggagaaa
     2461 ataccgcatc aggaaattgt aaacgttaat attttgttaa aattcgcgtt aaatttttgt
     2521 taaatcagct cattttttaa ccaataggcc gaaatcggca aaatccctta taaatcaaaa
     2581 gaatagaccg agatagggtt gagtgttgtt ccagtttgga acaagagtcc actattaaag
     2641 aacgtggact ccaacgtcaa agggcgaaaa accgtctatc agggcgatgg cccactacgt
     2701 gaaccatcac cctaatcaag ttttttgggg tcgaggtgcc gtaaagcact aaatcggaac
     2761 cctaaaggga gcccccgatt tagagcttga cggggaaagc cggcgaacgt ggcgagaaag
     2821 gaagggaaga aagcgaaagg agcgggcgct agggcgctgg caagtgtagc ggtcacgctg
     2881 cgcgtaacca ccacacccgc cgcgcttaat gcgccgctac agggcgcgtc gcgccattcg
     2941 ccattcaggc tgcgcaactg ttgggaaggg cgatcggtgc gggcctcttc gctattacgc
     3001 cagctggcga aagggggatg tgctgcaagg cgattaagtt gggtaacgcc agggttttcc
     3061 cagtcacgac gttgtaaaac gacggccagt gagcgcgcgt aatacgactc actatagggc
     3121 gaattgggta ccgggccccc cctcgaggtc gacggtatcg ataagcttga tatcgaattc
     3181 gcacttttcg gccaatggtc ttggtaattc ctttgcgcta gaattgaact caggtacaat
     3241 cacttcttct gaatgagatt tagtcattat agttttttct ccttgacgtt aaagtataga
     3301 ggtatattaa caattttttg ttgatacttt tattacattt gaataagaag taatacaaac
     3361 cgaaaatgtt gaaagtatta gttaaagtgg ttatgcagtt tttgcattta tatatctgtt
     3421 aatagatcaa aaatcatcgc ttcgctgatt aattacccca gaaataaggc taaaaaacta
     3481 atcgcattat catcctatgg ttgttaattt gattcgttca tttgaaggtt tgtggggcca
     3541 ggttactgcc aatttttcct cttcataacc ataaaagcta gtattgtaga atctttattg
     3601 ttcggagcag tgcggcgcga ggcacatctg cgtttcagga acgcgaccgg tgaagacgag
     3661 gacgcacgga ggagagtctt ccttcggagg gctgtcaccc gctcggcggc ttctaatccg
     3721 tacttcaata tagcaatgag cagttaagcg tattactgaa agttccaaag agaaggtttt
     3781 tttaggctaa gataatgggg ctctttacat ttccacaaca tataagtaag attagatatg
     3841 gatatgtata tggatatgta tatggtggta atgccatgta atatgattat taaacttctt
     3901 tgcgtccatc caaaaaaaaa gtaagaattt ttgaaaattc aatataaatg acagctcagt
     3961 tacaaagtga aagtacttct aaaattgttt tggttacagg tggtgctgga tacattggtt
     4021 cacacactgt ggtagagcta attgagaatg gatatgactg tgttgttgct gataacctgt
     4081 cgaattcctg cagcccgggc atatgctcaa cgaggtgcac gtgaacgcag acccgaacag
     4141 catctccacc ttggatgagc cggtgcttga gacgctgctg cgcgatgcca aggcgatcgg
     4201 ccgcaagctc gtcgtggttg tgtgcccctc tctaggaggt gataaggagc ttcgcgattg
     4261 ggatctctgg gggccgctgt tcctgtgtct cattctggct tccatcctca ccatcaacgc
     4321 cagcgacgac caaggcgccg ccgtcttctc tgcggtcttc atctttgtgt ggcttggcgg
     4381 tcttgtcgtc accgtcaatg ccaagctcct tggcagcaag atcatgttct tccaaacgta
     4441 ctgcgccatg ggctactgct tggcgcccat ctgtctcggc gctctcctgt gctgcgtcct
     4501 tccgtggttt ttattgaacc tcatgctgtg cttcatggcg tgggcgtggg cctgttgggc
     4561 ggccttgcgc tttttccgac acaccgtcag cgccgaccgt gaggtgttgg ttgtgtaccc
     4621 tgtcgggctc ttctacgtct tcttcacctg gatggttctg gttggtattt aggatccact
     4681 agttctagag cggccgccac cgcggtggag ctccagcttt tgttcccttt agtgagggtt
     4741 aattgcgcgc ttggcgtaat catggtcata gctgtttcct gtgtgaaatt gttatccgct
     4801 cacaattcca cacaacatag gagccggaag cataaagtgt aaagcctggg gtgcctaatg
     4861 agtgaggtaa ctcacattaa ttgcgttgcg ctcactgccc gctttccagt cgggaaacct
     4921 gtcgtgccag ctgcattaat gaatcggcca acgcgcgggg agaggcggtt tgcgtattgg
     4981 gcgctcttcc gcttcctcgc tcactgactc gctgcgctcg gtcgttcggc tgcggcgagc
     5041 ggtatcagct cactcaaagg cggtaatacg gttatccaca gaatcagggg ataacgcagg
     5101 aaagaacatg tgagcaaaag gccagcaaaa ggccaggaac cgtaaaaagg ccgcgttgct
     5161 ggcgtttttc cataggctcc gcccccctga cgagcatcac aaaaatcgac gctcaagtca
     5221 gaggtggcga aacccgacag gactataaag ataccaggcg tttccccctg gaagctccct
     5281 cgtgcgctct cctgttccga ccctgccgct taccggatac ctgtccgcct ttctcccttc
     5341 gggaagcgtg gcgctttctc atagctcacg ctgtaggtat ctcagttcgg tgtaggtcgt
     5401 tcgctccaag ctgggctgtg tgcacgaacc ccccgttcag cccgaccgct gcgccttatc
     5461 cggtaactat cgtcttgagt ccaacccggt aagacacgac ttatcgccac tggcagcagc
     5521 cactggtaac aggattagca gagcgaggta tgtaggcggt gctacagagt tcttgaagtg
     5581 gtggcctaac tacggctaca ctagaaggac agtatttggt atctgcgctc tgctgaagcc
     5641 agttaccttc ggaaaaagag ttggtagctc ttgatccggc aaacaaacca ccgctggtag
     5701 cggtggtttt tttgtttgca agcagcagat tacgcgcaga aaaaaaggat ctcaagaaga
     5761 tcctttgatc ttttctacgg ggtctgacgc tcagtggaac gaaaactcac gttaagggat
     5821 tttggtcatg agattatcaa aaaggatctt cacctagatc cttttaaatt aaaaatgaag
     5881 ttttaaatca atctaaagta tatatgagta aacttggtct gacagttacc aatgcttaat
     5941 cagtgaggca cctatctcag cgatctgtct atttcgttca tccatagttg cctgactccc
     6001 cgtcgtgtag ataactacga tacgggaggg cttaccatct ggccccagtg ctgcaatgat
     6061 accgcgagac ccacgctcac cggctccaga tttatcagca ataaaccagc cagccggaag
     6121 ggccgagcgc agaagtggtc ctgcaacttt atccgcctcc atccagtcta ttaattgttg
     6181 ccgggaagct agagtaagta gttcgccagt taatagtttg cgcaacgttg ttgccattgc
     6241 tacaggcatc gtggtgtcac gctcgtcgtt tggtatggct tcattcagct ccggttccca
     6301 acgatcaagg cgagttacat gatcccccat gttgtgcaaa aaagcggtta gctccttcgg
     6361 tcctccgatc gttgtcagaa gtaagttggc cgcagtgtta tcactcatgg ttatggcagc
     6421 actgcataat tctcttactg tcatgccatc cgtaagatgc ttttctgtga ctggtgagta
     6481 ctcaaccaag tcattctgag aatagtgtat gcggcgaccg agttgctctt gcccggcgtc
     6541 aatacgggat aataccgcgc cacatagcag aactttaaaa gtgctcatca ttggaaaacg
     6601 ttcttcgggg cgaaaactct caaggatctt accgctgttg agatccagtt cgatgtaacc
     6661 cactcgtgca cccaactgat cttcagcatc ttttactttc accagcgttt ctgggtgagc
     6721 aaaaacagga aggcaaaatg ccgcaaaaaa gggaataagg gcgacacgga aatgttgaat
     6781 actcatactc ttcctttttc aatattattg aagcatttat cagggttatt gtctcatgag
     6841 cggatacata tttgaatgta tttagaaaaa taaacaaata ggggttccgc gcacatttcc
     6901 ccgaaaagtg ccacctgggt ccttttcatc acgtgctata aaaataatta taatttaaat
     6961 tttttaatat aaatatataa attaaaaata gaaagtaaaa aaagaaatta aagaaaaaat
     7021 agtttttgtt ttccgaagat gtaaaagact ctagggggat cgccaacaaa tactaccttt
     7081 tatcttgctc ttcctgctct caggtattaa tgccgaattg tttcatcttg tctgtgtaga
     7141 agaccacaca cgaaaatcct gtgattttac attttactta tcgttaatcg aatgtatatc
     7201 tatttaatct gcttttcttg tctaataaat atatatgtaa agtacgcttt ttgttgaaat
     7261 tttttaaacc tttgtttatt tttttttctt cattccgtaa ctcttctacc ttctttattt
     7321 actttctaaa atccaaatac aaaacataaa aataaataaa cacagagtaa attcccaaat
     7381 tattccatca ttaaaagata cgaggcgcgt gtaagttaca ggcaagcgat ccgtcctaag
     7441 aaaccattat tatcatgaca ttaacctata aaaataggcg tatcacgagg ccctttcgtc
//
