LOCUS       V003599                 6333 bp    DNA     circular SYN 18-DEC-2018
DEFINITION  Exported.
ACCESSION   V003599
VERSION     V003599
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
            .
REFERENCE   1  (bases 1 to 6333)
  AUTHORS   Tomlin GC, Wixon JL, Bolotin-Fukuhara M, Oliver SG.
  TITLE     A new family of yeast vectors and S288C-derived strains for the
            systematic analysis of gene function
  JOURNAL   Yeast 18 (6), 563-575 (2001)
   PUBMED   11284012
REFERENCE   2  (bases 1 to 6333)
  AUTHORS   Tomlin GC, Wixon JL, Bolotin-Fukuhara M, Oliver SG.
  TITLE     Direct Submission
  JOURNAL   Submitted (02-AUG-1999) Biological Sciences, University of
            Manchester, 2.205 Stopford Building, Oxford Rd, Manchester M13 9PT,
            United Kingdom
REFERENCE   3  (bases 1 to 6333)
  TITLE     Direct Submission
REFERENCE   4  (bases 1 to 6333)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Yeast";
            date: "2001"; volume: "18"; issue: "6"; pages: "563-575"
            SGRef: number: 2; type: "Journal Article"; journalName: "Submitted
            (02-AUG-1999) Biological Sciences, University of Manchester, 2.205
            Stopford Building, Oxford Rd, Manchester M13 9PT, United Kingdom"
            SGRef: number: 3; type: "Journal Article"
FEATURES             Location/Qualifiers
     source          1..6333
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     CDS             1188..1829
                     /gene="ADE8"
                     /label="Phosphoribosylglycinamide formyltransferase"
                     /note="Phosphoribosylglycinamide formyltransferase from
                     Saccharomyces cerevisiae (strain ATCC 204508 / S288c).
                     Accession#: P04161"
     rep_origin      complement(2790..3245)
                     /direction=LEFT
                     /label="f1 ori"
                     /note="f1 bacteriophage origin of replication; arrow
                     indicates direction of (+) strand synthesis"
     primer_bind     3386..3402
                     /label="M13 fwd"
                     /note="common sequencing primer, one of multiple similar
                     variants"
     promoter        3412..3430
                     /label="T7 promoter"
                     /note="promoter for bacteriophage T7 RNA polymerase"
     misc_feature    complement(3439..3546)
                     /label="MCS"
                     /note="pBluescript multiple cloning site"
     promoter        complement(3559..3577)
                     /label="T3 promoter"
                     /note="promoter for bacteriophage T3 RNA polymerase"
     primer_bind     complement(3598..3614)
                     /label="M13 rev"
                     /note="common sequencing primer, one of multiple similar
                     variants"
     protein_bind    complement(3622..3638)
                     /label="lac operator"
                     /note="The lac repressor binds to the lac operator to
                     inhibit transcription in E. coli. This inhibition can be
                     relieved by adding lactose or
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(3646..3676)
                     /label="lac promoter"
                     /note="promoter for the E. coli lac operon"
     protein_bind    complement(3691..3712)
                     /label="CAP binding site"
                     /note="CAP binding activates transcription in the presence
                     of cAMP."
     rep_origin      complement(4000..4588)
                     /direction=LEFT
                     /label="ori"
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
                     replication"
     CDS             complement(4762..5619)
                     /label="AmpR"
                     /note="beta-lactamase"
     promoter        complement(5620..5724)
                     /label="AmpR promoter"
     misc_feature    5761..6264
                     /label="CEN/ARS"
                     /note="S. cerevisiae CEN6 centromere fused to an
                     autonomously replicating sequence"
ORIGIN
        1 tcgcgcgttt cggtgatgac ggtgaaaacc tctgacacat gcagctcccg gagacggtca
       61 cagcttgtct gtaagcggat gccgggagca gacaagcccg tcagggcgcg tcagcgggtg
      121 ttggcgggtg tcggggctgg cttaactatg cggcatcaga gcagattgta ctgagagtgc
      181 accataccac cttttcaatt catcattttt tttttattct tttttttgat ttcggtttct
      241 ttgaaatttt tttgattcgg taatctccga acagaaggaa gaacgaagga aggagcacag
      301 acttagattg gtatatatac gcatactaga ggatctttat aaacaggcaa aggttcattc
      361 tttctgatcc gttctatcag aaatttaacg gcttgaactc tataaggatc agacttgcct
      421 actactaaag cgttctgcaa gaaattcctg ataaggtcct gtagaacggc cttgcgaccg
      481 gaaaccggga aagatacaga cctgcaaata tccttcaatt cggacacttt cagcagttcc
      541 atcagagtga tggtttcctc cacctcgttt cttagttcat tagtcggttc cctatcaggc
      601 ccaggcgttt catcttccca gtaatcctct aaatttatca ttgaaacaga atgtatatgg
      661 aacactcaac tgtttgagtt ggagtcttct tttcttgtga aatatggatg aactccactt
      721 cattctttca tattcatatt aaagatgata cacttttcaa cttactatct ggtgcggtga
      781 atgggtgtat acattgacta cagctcaggt gacatctagg ggccgtttag tgtaagaata
      841 taaagtacaa tccaaaaagg aaaattatac ttgtttaaga aaagaggaac cttctgctcg
      901 ctctaaaatg gtagttctag tgatagttta aaaatgcatt ttccttccga cttcgaagat
      961 agatttcagc ataaagacta aaagcgtcag caaggattcg aatgagtgac tcgttgctag
     1021 tgactctgac ctgtttttta ttccagcaag aggaaagtta tatagagtag tttatgaaga
     1081 gaagacactt ctaaagttga ataacgctcc tagcctcaag gttgatgatt taacttgcag
     1141 caagcgcagg tgagagccaa cacacatcaa taatctttcc aaaagctatg gccagaattg
     1201 tcgtattaat ttcgggctca ggttccaact tgcaagcctt gatcgatgcc caaaagcagg
     1261 gccaattagg cgaggatgct catattgtct ctgtcatatc ttccagcaag aaggcatacg
     1321 ggttaactag ggcagcggac aataatatcc ccacaaaggt ttgttcgcta tacccatata
     1381 caaagggtat tgcgaaggag gacaaagccg caagggccaa ggcaagatcc cagtttgaga
     1441 acgacctcgc aaaactagtg ctcgaggaaa agcctgacgt tatcatctgc gcgggctggt
     1501 tattgatcct tggatctact ttcttgagcc aactgcaatc tgtgccgata ttaaatctgc
     1561 atccagcgct accaggttgt ttcgatggta ccacacacgc aattgaaatg gcatggcgca
     1621 aatgtcagga cgagaacaag cctctgacgg ccggatgcat ggtgcactat gtcatcgagg
     1681 aggtcgacaa gggtgagcca ttggtggtaa aaaagctaga aatcatacct ggcgaagaaa
     1741 cactagagca gtacgagcaa agggtacacg atgctgagca catcgcgatt gtagaagcta
     1801 catataaggt tttacagcag cttcacaaat aagaatagtt cccatattta tacttttata
     1861 tttggcattc ttcaaagtga ggcgcgaaac atctcgcgtc gtaaatcatg atcatggatt
     1921 gtgacaaaac gatcttaaag gtttcgaata aaatgtgtgt aaatacgtat acttatatat
     1981 gaatgtgcat gtgtaaacgc ttaatcgtgt tattgcaaaa ctaaaatcag gccagattgt
     2041 agtttaaacg gttctagtat aactggttta aggtgcggct cacaacctgt tacagggaga
     2101 caaaaaccat gaatattctt aagcatttcc caagctatgt tggcccatct aagattagga
     2161 ccttagtgat accaattggc cattggacgc gaaaagagtt taataatgcg gtacagaaac
     2221 tttctgaatt aattcaggta ttgttagcgg tttgaagcag gcggcagaag aagtaacaaa
     2281 ggaacctaga ggccttttga tgttagcaga attgtcatgc aagggctccc tatctactgg
     2341 agaatatact aagggtactg ttgacattgc gaagagcgac aaagattttg ttatcggctt
     2401 tattgctcaa agagacatgg gtggaagaga tgaaggttac gattggttga ttatgacacc
     2461 cggtgtgggt ttagatgaca agggagacgc attgggtcaa cagtatagaa ccgtggatga
     2521 tgtggtctct acaggatctg acattattat tgttggaaga ggactatttg caaagggaag
     2581 ggatgctaag gtagagggtg aacgttacag aaaagcaggc tgggaagcat atttgagaag
     2641 atgcggccag caaaactaaa aaactgtatt ataagtaaat gcatgtatac taaactcaca
     2701 aattagagct tcaatttaat tatatcagtt attaccctat gcggtgtgaa ataccgcaca
     2761 gatgcgtaag gagaaaatac cgcatcagga aattgtaaac gttaatattt tgttaaaatt
     2821 cgcgttaaat ttttgttaaa tcagctcatt ttttaaccaa taggccgaaa tcggcaaaat
     2881 cccttataaa tcaaaagaat agaccgagat agggttgagt gttgttccag tttggaacaa
     2941 gagtccacta ttaaagaacg tggactccaa cgtcaaaggg cgaaaaaccg tctatcaggg
     3001 cgatggccca ctacgtgaac catcacccta atcaagtttt ttggggtcga ggtgccgtaa
     3061 agcactaaat cggaacccta aagggagccc ccgatttaga gcttgacggg gaaagccggc
     3121 gaacgtggcg agaaaggaag ggaagaaagc gaaaggagcg ggcgctaggg cgctggcaag
     3181 tgtagcggtc acgctgcgcg taaccaccac acccgccgcg cttaatgcgc cgctacaggg
     3241 cgcgtccatt cgccattcag gctgcgcaac tgttgggaag ggcgatcggt gcgggcctct
     3301 tcgctattac gccagctggc gaaaggggga tgtgctgcaa ggcgattaag ttgggtaacg
     3361 ccagggtttt cccagtcacg acgttgtaaa acgacggcca gtgagcgcgc gtaatacgac
     3421 tcactatagg gcgaattggg taccgggccc cccctcgagg tcgacggtat cgataagctt
     3481 gatatcgaat tcctgcagcc cgggggatcc actagttcta gagcggccgc caccgcggtg
     3541 gagctccagc ttttgttccc tttagtgagg gttaattgcg cgcttggcgt aatcatggtc
     3601 atagctgttt cctgtgtgaa attgttatcc gctcacaatt ccacacaaca taggagccgg
     3661 aagcataaag tgtaaagcct ggggtgccta atgagtgagg taactcacat taattgcgtt
     3721 gcgctcactg cccgctttcc agtcgggaaa cctgtcgtgc cagctgcatt aatgaatcgg
     3781 ccaacgcgcg gggagaggcg gtttgcgtat tgggcgctct tccgcttcct cgctcactga
     3841 ctcgctgcgc tcggtcgttc ggctgcggcg agcggtatca gctcactcaa aggcggtaat
     3901 acggttatcc acagaatcag gggataacgc aggaaagaac atgtgagcaa aaggccagca
     3961 aaaggccagg aaccgtaaaa aggccgcgtt gctggcgttt ttccataggc tccgcccccc
     4021 tgacgagcat cacaaaaatc gacgctcaag tcagaggtgg cgaaacccga caggactata
     4081 aagataccag gcgtttcccc ctggaagctc cctcgtgcgc tctcctgttc cgaccctgcc
     4141 gcttaccgga tacctgtccg cctttctccc ttcgggaagc gtggcgcttt ctcatagctc
     4201 acgctgtagg tatctcagtt cggtgtaggt cgttcgctcc aagctgggct gtgtgcacga
     4261 accccccgtt cagcccgacc gctgcgcctt atccggtaac tatcgtcttg agtccaaccc
     4321 ggtaagacac gacttatcgc cactggcagc agccactggt aacaggatta gcagagcgag
     4381 gtatgtaggc ggtgctacag agttcttgaa gtggtggcct aactacggct acactagaag
     4441 gacagtattt ggtatctgcg ctctgctgaa gccagttacc ttcggaaaaa gagttggtag
     4501 ctcttgatcc ggcaaacaaa ccaccgctgg tagcggtggt ttttttgttt gcaagcagca
     4561 gattacgcgc agaaaaaaag gatctcaaga agatcctttg atcttttcta cggggtctga
     4621 cgctcagtgg aacgaaaact cacgttaagg gattttggtc atgagattat caaaaaggat
     4681 cttcacctag atccttttaa attaaaaatg aagttttaaa tcaatctaaa gtatatatga
     4741 gtaaacttgg tctgacagtt accaatgctt aatcagtgag gcacctatct cagcgatctg
     4801 tctatttcgt tcatccatag ttgcctgact ccccgtcgtg tagataacta cgatacggga
     4861 gggcttacca tctggcccca gtgctgcaat gataccgcga gacccacgct caccggctcc
     4921 agatttatca gcaataaacc agccagccgg aagggccgag cgcagaagtg gtcctgcaac
     4981 tttatccgcc tccatccagt ctattaattg ttgccgggaa gctagagtaa gtagttcgcc
     5041 agttaatagt ttgcgcaacg ttgttgccat tgctacaggc atcgtggtgt cacgctcgtc
     5101 gtttggtatg gcttcattca gctccggttc ccaacgatca aggcgagtta catgatcccc
     5161 catgttgtgc aaaaaagcgg ttagctcctt cggtcctccg atcgttgtca gaagtaagtt
     5221 ggccgcagtg ttatcactca tggttatggc agcactgcat aattctctta ctgtcatgcc
     5281 atccgtaaga tgcttttctg tgactggtga gtactcaacc aagtcattct gagaatagtg
     5341 tatgcggcga ccgagttgct cttgcccggc gtcaatacgg gataataccg cgccacatag
     5401 cagaacttta aaagtgctca tcattggaaa acgttcttcg gggcgaaaac tctcaaggat
     5461 cttaccgctg ttgagatcca gttcgatgta acccactcgt gcacccaact gatcttcagc
     5521 atcttttact ttcaccagcg tttctgggtg agcaaaaaca ggaaggcaaa atgccgcaaa
     5581 aaagggaata agggcgacac ggaaatgttg aatactcata ctcttccttt ttcaatatta
     5641 ttgaagcatt tatcagggtt attgtctcat gagcggatac atatttgaat gtatttagaa
     5701 aaataaacaa ataggggttc cgcgcacatt tccccgaaaa gtgccacctg ggtccttttc
     5761 atcacgtgct ataaaaataa ttataattta aattttttaa tataaatata taaattaaaa
     5821 atagaaagta aaaaaagaaa ttaaagaaaa aatagttttt gttttccgaa gatgtaaaag
     5881 actctagggg gatcgccaac aaatactacc ttttatcttg ctcttcctgc tctcaggtat
     5941 taatgccgaa ttgtttcatc ttgtctgtgt agaagaccac acacgaaaat cctgtgattt
     6001 tacattttac ttatcgttaa tcgaatgtat atctatttaa tctgcttttc ttgtctaata
     6061 aatatatatg taaagtacgc tttttgttga aattttttaa acctttgttt attttttttt
     6121 cttcattccg taactcttct accttcttta tttactttct aaaatccaaa tacaaaacat
     6181 aaaaataaat aaacacagag taaattccca aattattcca tcattaaaag atacgaggcg
     6241 cgtgtaagtt acaggcaagc gatccgtcct aagaaaccat tattatcatg acattaacct
     6301 ataaaaatag gcgtatcacg aggccctttc gtc
//
