LOCUS       40924_37728        4265 bp DNA     circular SYN 18-DEC-2018
DEFINITION  Cloning vector pRS002, complete sequence.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 4265)
  AUTHORS   Ghosh IN, Landick R.
  TITLE     OptSSeq: High-throughput sequencing readout of growth enrichment 
            defines optimal gene expression elements for homoethanologenesis
  JOURNAL   ACS Synth Biol (2016) In press
  PUBMED    27404024
REFERENCE   2  (bases 1 to 4265)
  AUTHORS   Ghosh IN, Landick RC.
  TITLE     Direct Submission
  JOURNAL   Submitted (15-JUL-2016) DOE Great Lakes Bioenergy Research Center, 
            University of Wisconsin-Madison, 1552 University Avenue, Madison, WI
            53726, United States
REFERENCE   3  (bases 1 to 4265)
  TITLE     Direct Submission
REFERENCE   4  (bases 1 to 4265)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "ACS Synth 
            Biol (2016) In press"
COMMENT     SGRef: number: 2; type: "Journal Article"; journalName: "Submitted 
            (15-JUL-2016) DOE Great Lakes Bioenergy Research Center, University 
            of Wisconsin-Madison, 1552 University Avenue, Madison, WI 53726, 
            United States"
COMMENT     SGRef: number: 3; type: "Journal Article"
FEATURES             Location/Qualifiers
     source          1..4265
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        1903..1994
                     /label=AmpR promoter
     CDS             1995..2783
                     /codon_start=1
                     /label=SmR
                     /note="aminoglycoside adenylyltransferase (Murphy, 1985)"
                     /translation="MREAVIAEVSTQLSEVVGVIERHLEPTLLAVHLYGSAVDGGLKPH
                     SDIDLLVTVTVRLDETTRRALINDLLETSASPGESEILRAVEVTIVVHDDIIPWRYPAK
                     RELQFGEWQRNDILAGIFEPATIDIDLAILLTKAREHSVALVGPAAEELFDPVPEQDLF
                     EALNETLTLWNSPPDWAGDERNVVLTLSRIWYSAVTGKIAPKDVAADWAMERLPAQYQP
                     VILEARQAYLGQEEDRLASRADQLEEFVHYVKGEITKVVGK"
     CDS             complement(3361..4020)
                     /codon_start=1
                     /label=pBBR1 Rep
                     /note="replication protein for the broad-host-range plasmid
                     pBBR1 from Bordetella bronchiseptica"
                     /translation="MATQSREIGIQAKNKPGHWVQTERKAHEAWAGLIARKPTAAMLLH
                     HLVAQMGHQNAVVVSQKTLSKLIGRSLRTVQYAVKDLVAERWISVVKLNGPGTVSAYVV
                     NDRVAWGQPRDQLRLSVFSAAVVVDHDDQDESLLGHGDLRRIPTLYPGEQQLPTGPGEE
                     PPSQPGIPGMEPDLPALTETEEWERRGQQRLPMPDEPCFLDDGEPLEPPTRVTLPRR"
     rep_origin      complement(join(4021..4265,1..525))
                     /direction=LEFT
                     /label=pBBR1 oriV
                     /note="replication origin of the broad-host-range plasmid
                     pBBR1 from Bordetella bronchiseptica; requires the pBBR1 
                     Rep protein for replication"
ORIGIN
        1 agaagggcac ccctgcgggg ttcccccacg ccttcggcgc ggctccccct ccggcaaaaa
       61 gtggcccctc cggggcttgt tgatcgactg cgcggccttc ggccttgccc aaggtggcgc
      121 tgcccccttg gaacccccgc actcgccgcc gtgaggctcg gggggcaggc gggcgggctt
      181 cgccttcgac tgcccccact cgcataggct tgggtcgttc caggcgcgtc aaggccaagc
      241 cgctgcgcgg tcgctgcgcg agccttgacc cgccttccac ttggtgtcca accggcaagc
      301 gaagcgcgca ggccgcaggc cggaggcttt tccccagaga aaattaaaaa aattgatggg
      361 gcaaggccgc aggccgcgca gttggagccg gtgggtatgt ggtcgaaggc tgggtagccg
      421 gtgggcaatc cctgtggtca agctcgtggg caggcgcagc ctgtccatca gcttgtccag
      481 cagggttgtc cacgggccga gcgaagcgag ccagccggtg gccgctcgcg gccatcgtcc
      541 acatatccac gggctggcaa gggagcgcag cgaccgcgca gggcgaagcc cggagagcaa
      601 gcccgtaggg cgccgcagcc gccgtaggcg gtcacgactt tgcgaagcaa agtctagtga
      661 gtatactcaa gcattgagtg gcccgccgga ggcaccgcct tgcgctgccc ccgtcgagcc
      721 ggttggacac caaaagggag gggcaggcat ggcggcatac gcgatcatgc gatgcaagaa
      781 gctggcgaaa atgggcaacg tggcggccag tctcaagcac gcctaccgcg agcgcgagac
      841 gcccaacgct gacgccagca ggacgccaga gaacgagcac tgggcggcca gcagcaccga
      901 tgaagcgatg ggccgactgc gcgagttgct gccagagaag cggcgcaagg acgctgtgtt
      961 ggcggtcgag tacgtcatga cggccagccc ggaatggtgg aagtcggcca gccaagaaca
     1021 gcaggcggcg ttcttcgaga aggcgcacaa gtggctggcg gacaagtacg gggcggatcg
     1081 catcgtgacg gccagcatcc accgtgacga aaccagcccg cacatgaccg cgttcgtggt
     1141 gccgctgacg caggacggca ggctgtcggc caaggagttc atcggcaaca aagcgcagat
     1201 gacccgcgac cagaccacgt ttgcggccgc tgtggccgat ctagggctgc aacggggcat
     1261 cgagggcagc aaggcacgtc acacgcgcat tcaggcgttc tacgaggccc tggagcggcc
     1321 accagtgggc cacgtcacca tcagcccgca agcggtcgag ccacgcgcct atgcaccgca
     1381 gggattggcc gaaaagctgg gaatctcaaa gcgcgttgag acgccggaag ccgtggccga
     1441 ccggctgaca aaagcggttc ggcaggggta tgagcctgcc ctacaggccg ccgcaggagc
     1501 gcgtgagatg cgcaagaagg ccgatcaagc ccaagagacg gcccgagacc ttcgggagcg
     1561 cctgaagccc gttctggacg ccctggggcc gttgaatcgg gatatgcagg ccaaggccgc
     1621 cgcgatcatc aaggccgtgg gcgaaaagct gctgacggaa cagcgggaag tccagcgcca
     1681 gaaacaggcc cagcgccagc aggaacgcgg gcgcgcacat ttccccgaaa agtgccacct
     1741 ggcggcgttg tgacaattta ccgaacaact ccgcggccgg gaagccgatc tcggcttgaa
     1801 cgaattcctg cttatccacg gaatctgtgg gtaactttgt atgtgtccgc agcgcccgcc
     1861 gcagtctcac gcccggagcg tagcgaccga gtgagctagc tatttgttta tttttctaaa
     1921 tacattcaaa tatgtatccg ctcatgagac aataaccctg ataaatgctt caataatatt
     1981 gaaaaaggaa gagtatgagg gaagcggtga tcgccgaagt atcgactcaa ctatcagagg
     2041 tagttggcgt catcgagcgc catctcgaac cgacgttgct ggccgtacat ttgtacggct
     2101 ccgcagtgga tggcggcctg aagccacaca gtgatattga tttgctggtt acggtgaccg
     2161 taaggcttga tgaaacaacg cggcgagctt tgatcaacga ccttttggaa acttcggctt
     2221 cccctggaga gagcgagatt ctccgcgctg tagaagtcac cattgttgtg cacgacgaca
     2281 tcattccgtg gcgttatcca gctaagcgcg aactgcaatt tggagaatgg cagcgcaatg
     2341 acattcttgc aggtatcttc gagccagcca cgatcgacat tgatctggct atcttgctga
     2401 caaaagcaag agaacatagc gttgccttgg taggtccagc ggcggaggaa ctctttgatc
     2461 cggttcctga acaggatcta tttgaggcgc taaatgaaac cttaacgcta tggaactcgc
     2521 cgcccgactg ggctggcgat gagcgaaatg tagtgcttac gttgtcccgc atttggtaca
     2581 gcgcagtaac cggcaaaatc gcgccgaagg atgtcgctgc cgactgggca atggagcgcc
     2641 tgccggccca gtatcagccc gtcatacttg aagctagaca ggcttatctt ggacaagaag
     2701 aagatcgctt ggcctcgcgc gcagatcagt tggaagaatt tgtccactac gtgaaaggcg
     2761 agatcaccaa ggtagtcggc aaataatgtc taacaattcg ttcaagccga ggggccgcaa
     2821 gatccggcca cgatgacccg gtcgtcggtt cagggcaggg tcgttaaata gccgcttatg
     2881 tctattgctg gtttaccggt ttattgacta ccggaagcag tgtgaccgtg tgcttcttac
     2941 tacgtcagtg tcgtgccata gactggaaag cgggcagtaa ctactcaatc ccttggcact
     3001 aaaagtgtca ggggattttt tatgttaata caccatcaca gaattgtgag cgctcacgat
     3061 atcgaattcc tgcagcccgg gggatcccta gttctagaaa taagttaaat aaagccctga
     3121 gtttaaccgc tcggggcttt ttgcgtttta agcacactag agcggccatg catcccagag
     3181 cctgataaaa acggtgaatc cgttagcgag gtgccgccgg cttccattca ggtcgaggtg
     3241 gcccggctcc atgcaccgcg acgcaacgcg gggaggcaga caaggtatag ggcggcgagg
     3301 cggctacagc cgatagtctg gaacagcgca cttacgggtt gctgcgcaac ccaagtgcta
     3361 ccggcgcggc agcgtgaccc gtgtcggcgg ctccaacggc tcgccatcgt ccagaaaaca
     3421 cggctcatcg ggcatcggca ggcgctgctg cccgcgccgt tcccattcct ccgtttcggt
     3481 caaggctggc aggtctggtt ccatgcccgg aatgccgggc tggctgggcg gctcctcgcc
     3541 ggggccggtc ggtagttgct gctcgcccgg atacagggtc gggatgcggc gcaggtcgcc
     3601 atgccccaac agcgattcgt cctggtcgtc gtgatcaacc accacggcgg cactgaacac
     3661 cgacaggcgc aactggtcgc ggggctggcc ccacgccacg cggtcattga ccacgtaggc
     3721 cgacacggtg ccggggccgt tgagcttcac gacggagatc cagcgctcgg ccaccaagtc
     3781 cttgactgcg tattggaccg tccgcaaaga acgtccgatg agcttggaaa gtgtcttctg
     3841 gctgaccacc acggcgttct ggtggcccat ctgcgccacg aggtgatgca gcagcattgc
     3901 cgccgtgggt ttcctcgcaa taagcccggc ccacgcctca tgcgctttgc gttccgtttg
     3961 cacccagtga ccgggcttgt tcttggcttg aatgccgatt tctctggact gcgtggccat
     4021 gcttatctcc atgcggtagg gtgccgcacg gttgcggcac catgcgcaat cagctgcaac
     4081 ttttcggcag cgcgacaaca attatgcgtt gcgtaaaagt ggcagtcaat tacagatttt
     4141 ctttaaccta cgcaatgagc tattgcgggg ggtgccgcaa tgagctgttg cgtacccccc
     4201 ttttttaagt tgttgatttt taagtctttc gcatttcgcc ctatatctag ttctttggtg
     4261 cccaa
//
