LOCUS       40924_37693       10276 bp DNA     circular SYN 18-DEC-2018
DEFINITION  Cloning vector pRR25, complete sequence.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 10276)
  AUTHORS   Ramsden R, Arms L, Davis TN, Muller EG.
  TITLE     An intein with genetically selectable markers provides a new 
            approach to internally label proteins with GFP
  JOURNAL   BMC Biotechnol. 11 (1), 71 (2011)
  PUBMED    21708017
REFERENCE   2  (bases 1 to 10276)
  AUTHORS   Ramsden R, Arms L, Davis TN, Muller EGD.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-JUN-2011) Biochemistry, University of Washington, 1705
            NE Pacific St, HSB J461, Seattle, WA 98195, USA
REFERENCE   3  (bases 1 to 10276)
  TITLE     Direct Submission
REFERENCE   4  (bases 1 to 10276)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "BMC 
            Biotechnol."; date: "2011"; volume: "11"; issue: "1"; pages: "71"
COMMENT     SGRef: number: 2; type: "Journal Article"; journalName: "Submitted 
            (30-JUN-2011) Biochemistry, University of Washington, 1705 NE 
            Pacific St, HSB J461, Seattle, WA 98195, USA"
COMMENT     SGRef: number: 3; type: "Journal Article"
FEATURES             Location/Qualifiers
     source          1..10276
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        14..678
                     /label=GAL1,10 promoter
                     /note="divergent inducible promoter, regulated by Gal4"
     CDS             694..714
                     /codon_start=1
                     /label=SV40 NLS
                     /note="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /translation="PKKKRKV"
     CDS             724..744
                     /codon_start=1
                     /label=SV40 NLS
                     /note="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /translation="PKKKRKV"
     CDS             748..768
                     /codon_start=1
                     /label=SV40 NLS
                     /note="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /translation="PKKKRKV"
     CDS             781..1491
                     /codon_start=1
                     /label=GFP (S65T)
                     /note="S65T variant of Aequorea victoria green fluorescent 
                     protein (Heim et al., 1995)"
                     /translation="SKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTLKF
                     ICTTGKLPVPWPTLVTTFTYGVQCFSRYPDHMKRHDFFKSAMPEGYVQERTIFFKDDGN
                     YKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIKVN
                     FKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLLEF
                     VTAAGITHGMDELYK"
     misc_feature    1540..2940
                     /label=Pch PRP8 intein with LexA::VP16
                     /note="Pch PRP8 intein with LexA::VP16"
     CDS             1906..2508
                     /codon_start=1
                     /gene="lexA"
                     /product="E. coli DNA-binding protein that represses genes
                     involved in the SOS response to DNA damage"
                     /label=LexA
                     /note="The N-terminal DNA-binding domain recognizes the
                     16-bp palindromic sequence 5'-CTGTATATATATACAG-3'."
                     /translation="KALTARQQEVFDLIRDHISQTGMPPTRAEIAQRLGFRSPNAAEEH
                     LKALARKGVIEIVSGASRGIRLLQEEEEGLPLVGRVAAGEPLLAQQHIEGHYQVDPSLF
                     KPNADFLLRVSGMSMKDIGIMDGDLLAVHKTQDVRNGQVVVARIDDEVTVKRLKKQGNK
                     VELLPENSEFKPIVVDLRQQSFTIEGLAVGVIRNGDWL"
     CDS             2554..2787
                     /codon_start=1
                     /gene="UL48"
                     /product="transcriptional activation domain of herpes
                     simplex virus protein VP16 (Triezenberg et al., 1988; 
                     Cousens et al., 1989)"
                     /label=VP16 AD
                     /translation="APPTDVSLGDELHLDGEDVAMAHADALDDFDLDMLGDGDSPGPGF
                     TPHDSAPYGALDMADFEFEQMFTDALGIDEYGG"
     CDS             2953..2976
                     /codon_start=1
                     /label=FLAG
                     /note="FLAG(R) epitope tag, followed by an enterokinase
                     cleavage site"
                     /translation="DYKDDDDK"
     promoter        3766..3986
                     /label=URA3 promoter
     CDS             3987..4787
                     /codon_start=1
                     /label=URA3
                     /note="orotidine-5'-phosphate decarboxylase, required for
                     uracil biosynthesis"
                     /translation="MSKATYKERAATHPSPVAAKLFNIMHEKQTNLCASLDVRTTKELL
                     ELVEALGPKICLLKTHVDILTDFSMEGTVKPLKALSAKYNFLLFEDRKFADIGNTVKLQ
                     YSAGVYRIAEWADITNAHGVVGPGIVSGLKQAAEEVTKEPRGLLMLAELSCKGSLSTGE
                     YTKGTVDIAKSDKDFVIGFIAQRDMGGRDEGYDWLIMTPGVGLDDKGDALGQQYRTVDD
                     VVSTGSDIIIVGRGLFAKGRDAKVEGERYRKAGWEAYLRRCGQQN"
     misc_feature    complement(6135..7984)
                     /label=CEN/ARS
                     /note="S. cerevisiae CEN4 centromere fused to the
                     autonomously replicating sequence ARS1/ARS416"
     misc_feature    8126..8266
                     /label=bom
                     /note="basis of mobility region from pBR322"
     rep_origin      complement(8452..9040)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     CDS             complement(9214..10071)
                     /codon_start=1
                     /label=AmpR
                     /note="beta-lactamase"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRVDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPAAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     promoter        complement(10072..10176)
                     /label=AmpR promoter
ORIGIN
        1 gaattccttg aattttcaaa aattcttact ttttttttgg atggacgcaa agaagtttaa
       61 taatcatatt acatggcatt accaccatat acatatccat atacatatcc atatctaatc
      121 ttacttatat gttgtggaaa tgtaaagagc cccattatct tagcctaaaa aaaccttctc
      181 tttggaactt tcagtaatac gcttaactgc tcattgctat attgaagtac ggattagaag
      241 ccgccgagcg ggtgacagcc ctccgaagga agactctcct ccgtgcgtcc tcgtcttcac
      301 cggtcgcgtt cctgaaacgc agatgtgcct cgcgccgcac tgctccgaac aataaagatt
      361 ctacaatact agcttttatg gttatgaaga ggaaaaattg gcagtaacct ggccccacaa
      421 accttcaaat gaacgaatca aattaacaac cataggatga taatgcgatt agttttttag
      481 ccttatttct ggggtaatta atcagcgaag cgatgatttt tgatctatta acagatatat
      541 aaatgcaaaa actgcataac cactttaact aatactttca acattttcgg tttgtattac
      601 ttcttattca aatgtaataa aagtatcaac aaaaaattgt taatatacct ctatacttta
      661 acgtcaagga gaaaaaaccc cggatccatg gttccaaaga agaagagaaa ggttttaatt
      721 gatccaaaaa agaaaagaaa ggttgatcca aagaaaaaga gaaaagtttt aattaaagga
      781 agtaaaggag aagaactttt cactggagtt gtcccaattc ttgttgaatt agatggtgat
      841 gttaatgggc acaaattttc tgtcagtgga gagggtgaag gtgatgcaac atacggaaaa
      901 cttaccctta aatttatttg cactactgga aaactacctg ttccatggcc aacacttgtc
      961 actactttca cttatggtgt tcaatgcttt tcaagatacc cagatcatat gaaacggcat
     1021 gactttttca agagtgccat gcccgaaggt tatgtacagg aaagaactat atttttcaaa
     1081 gatgacggga actacaagac acgtgctgaa gtcaagtttg aaggtgatac ccttgttaat
     1141 agaatcgagt taaaaggtat tgattttaaa gaagatggaa acattcttgg acacaaattg
     1201 gaatacaact ataactcaca caatgtatac atcatggcag acaaacaaaa gaatggaatc
     1261 aaagttaact tcaaaattag acacaacatt gaagatggaa gcgttcaact agcagaccat
     1321 tatcaacaaa atactccaat tggcgatggc cctgtccttt taccagacaa ccattacctg
     1381 tccacacaat ctgccctttc gaaagatccc aacgaaaaga gagaccacat ggtccttctt
     1441 gagtttgtaa cagctgctgg gattacacat ggcatggatg aactatacaa aggtgctggt
     1501 gcaggtgcag gagctggagc aggtttctgg gagaaagcgt gtttggccaa gggaacccgt
     1561 ttgttgagat gcgatggaac cgagatcaat gtggaagacg tgcgcgaagg tgacctactt
     1621 ctgggtcccg atggagagcc tcgccgtgca ttcaacatag tgaatggcat cgacagactg
     1681 tacagaatca agatcggcgg tgagaaagag gaccttgtgg tgacgccgaa ccatattctg
     1741 gtgctttata gagaggatgg ttccaagaat gtggagaagc aaacggtgga gatcactgct
     1801 gccgagtttg ccgcgctttc taccgaggaa agaagcctct atagtgcctt tacatctcct
     1861 agggctgaga agggcgccgc aggtgcaggt gcaggtgcag ctagcaaagc gttaacggcc
     1921 aggcaacaag aggtgtttga tctcatccgt gatcacatca gccagacagg tatgccgccg
     1981 acgcgtgcgg aaatcgcgca gcgtttgggg ttccgttccc caaacgcggc tgaagaacat
     2041 ctgaaggcgc tggcacgcaa aggcgttatt gaaattgttt ccggcgcatc acgcgggatt
     2101 cgtctgttgc aggaagagga agaagggttg ccgctggtag gtcgtgtggc tgccggtgaa
     2161 ccacttctgg cgcaacagca tattgaaggt cattatcagg tcgatccttc cttattcaag
     2221 ccgaatgctg atttcctgct gcgcgtcagc gggatgtcga tgaaagatat cggcattatg
     2281 gatggtgact tgctggcagt gcataaaact caggatgtac gtaacggtca ggtcgttgtc
     2341 gcacgtattg atgacgaagt taccgttaag cgcctgaaaa aacagggcaa taaagtcgaa
     2401 ctgttgccag aaaatagcga gtttaaacca attgtcgtag atcttcgtca gcagagcttc
     2461 accattgaag ggctggcggt tggggttatt cgcaacggcg actggctgga attcccgggg
     2521 atccgtcgac ctgcaggaat tcccggggat ctggcccccc cgaccgatgt cagcctgggg
     2581 gacgagctcc acttagacgg cgaggacgtg gcgatggcgc atgccgacgc gctagacgat
     2641 ttcgatctgg acatgttggg ggacggggat tccccgggtc cgggatttac cccccacgac
     2701 tccgccccct acggcgctct ggatatggcc gacttcgagt ttgagcagat gtttaccgat
     2761 gcccttggaa ttgacgagta cggtgggggt accgcgggtg ctggtgctgg tgcttcggct
     2821 caaacgcaca gtttcaagat tgagcaagtt agcctcgaat ccgagaagac agagtgggct
     2881 ggtttccgag tcgacaaaga tcagctttac ctgcgtcatg actaccttgt cctgcacaac
     2941 agtggtttcg aagattacaa ggatgacgac gataagtaag tcgaccgatg cccttgagag
     3001 ccttcaaccc agtcagctcc ttccggtggg cgcggggcat gactatcgtc gccgcactta
     3061 tgactgtctt ctttatcatg caactcgtag gacaggtgcc ggcagcgctc tgggtcattt
     3121 tcggcgagga ccgctttcgc tggagcgcga cgatgatcgg cctgtcgctt gcggtattcg
     3181 gaatcttgca cgccctcgct caagccttcg tcactggtcc cgccaccaaa cgtttcggcg
     3241 agaagcaggc cattatcgcc ggcatggcgg ccgacgcgct gggctacgtc ttgctggcgt
     3301 tcgcgacgcg aggctggatg gccttcccca ttatgattct tctcgcttcc ggcggcatcg
     3361 ggatgcccgc gttgcaggcc atgctgtcca ggcaggtaga tgacgaccat cagggacagc
     3421 ttcaaggatc gctcgcggct cttaccagcc taacttcgat cactggaccg ctgatcgtca
     3481 cggcgattta tgccgcctcg gcgagcacat ggaacgggtt ggcatggatt gtaggcgccg
     3541 ccctatacct tgtctgcctc cccgcgttgc gtcgcggtgc atggagccgg gccacctcga
     3601 cctgaatgga agccggcggc acctcgctaa cggattcacc actccaagaa ttggagccaa
     3661 tcaattcttg cggagaactg tgaatgcgca aaccaaccct tggcagaaca tatccatcgc
     3721 gtccgccatc tccagcagcc gcacgcggcg catcgggggg ggggtttcaa ttcaattcat
     3781 catttttttt ttattctttt ttttgatttc ggtttctttg aaattttttt gattcggtaa
     3841 tctccgaaca gaaggaagaa cgaaggaagg agcacagact tagattggta tatatacgca
     3901 tatgtagtgt tgaagaaaca tgaaattgcc cagtattctt aacccaactg cacagaacaa
     3961 aaacctgcag gaaacgaaga taaatcatgt cgaaagctac atataaggaa cgtgctgcta
     4021 ctcatcctag tcctgttgct gccaagctat ttaatatcat gcacgaaaag caaacaaact
     4081 tgtgtgcttc attggatgtt cgtaccacca aggaattact ggagttagtt gaagcattag
     4141 gtcccaaaat ttgtttacta aaaacacatg tggatatctt gactgatttt tccatggagg
     4201 gcacagttaa gccgctaaag gcattatccg ccaagtacaa ttttttactc ttcgaagaca
     4261 gaaaatttgc tgacattggt aatacagtca aattgcagta ctctgcgggt gtatacagaa
     4321 tagcagaatg ggcagacatt acgaatgcac acggtgtggt gggcccaggt attgttagcg
     4381 gtttgaagca ggcggcagaa gaagtaacaa aggaacctag aggccttttg atgttagcag
     4441 aattgtcatg caagggctcc ctatctactg gagaatatac taagggtact gttgacattg
     4501 cgaagagcga caaagatttt gttatcggct ttattgctca aagagacatg ggtggaagag
     4561 atgaaggtta cgattggttg attatgacac ccggtgtggg tttagatgac aagggagacg
     4621 cattgggtca acagtataga accgtggatg atgtggtctc tacaggatct gacattatta
     4681 ttgttggaag aggactattt gcaaagggaa gggatgctaa ggtagagggt gaacgttaca
     4741 gaaaagcagg ctgggaagca tatttgagaa gatgcggcca gcaaaactaa aaaactgtat
     4801 tataagtaaa tgcatgtata ctaaactcac aaattagagc ttcaatttaa ttatatcagt
     4861 tattacccgg gaatctcggt cgtaatgatt tttataatga cgaaaaaaaa aaaattggaa
     4921 agaaaacccc ccccccgcag cgttgggtcc tggccacggg tgcgcatgat cgtgctcctg
     4981 tcgttgagga cccggctagg ctggcggggt tgccttactg gttagcagaa tgaatcaccg
     5041 atacgcgagc gaacgtgaag cgactgctgc tgcaaaacgt ctgcgacctg agcaacaaca
     5101 tgaatggtct tcggtttccg tgtttcgtaa agtctggaaa cgcggaagtc agcgccctgc
     5161 accattatgt tccggatctg catcgcagga tgctgctggc taccctgtgg aacacctaca
     5221 tctgtattaa cgaagcgctg gcattgaccc tgagtgattt ttctctggtc ccgccgcatc
     5281 cataccgcca gttgtttacc ctcacaacgt tccagtaacc gggcatgttc atcatcagta
     5341 acccgtatcg tgagcatcct ctctcgtttc atcggtatca ttacccccat gaacagaaat
     5401 tcccccttac acggaggcat caagtgacca aacaggaaaa aaccgccctt aacatggccc
     5461 gctttatcag aagccagaca ttaacgcttc tggagaaact caacgagctg gacgcggatg
     5521 aacaggcaga catctgtgaa tcgcttcacg accacgctga tgagctttac cgcaggtggg
     5581 ccattctcat gaagaatatc ttgaatttat tgtcatatta ctagttggtg tggaagtcca
     5641 tatatcggtg atcaatatag tggttgacat gctggctagt caacattgag ccttttgatc
     5701 atgcaaatat attacggtat tttacaatca aatatcaaac ttaactattg actttataac
     5761 ttatttaggt ggtaacattc ttataaaaaa gaaaaaaatt actgcaaaac agtactagct
     5821 tttaacttgt atcctaggtt atctatgctg tctcaccata gagaatatta cctatttcag
     5881 aatgtatgtc catgattcgc cgggtaaata catataatac acaaatctgg cttaataaag
     5941 tctataatat atctcataaa gaagtgctaa attggctagt gctatatatt tttaagaaaa
     6001 tttcttttga ctaagtccat atcgactttg taaaagttca ctttagcata catatattac
     6061 acgagccaga aattgtaact tttgcctaaa atcacaaatt gcaaaattta attgcttgca
     6121 aaaggtcaca tgcttataat caactttttt aaaaatttaa aatacttttt tattttttat
     6181 ttttaaacat aaatgaaata atttatttat tgtttatgat taccgaaaca taaaacctgc
     6241 tcaagaaaaa gaaactgttt tgtccttgga aaaaaagcac tacctaggag cggccaaaat
     6301 gccgaggctt tcatagctta aactctttac agaaaatagg cattatagat cagttcgagt
     6361 tttcttattc ttccttccgg ttttatcgtc acagttttac agtaaataag tatcacctct
     6421 tagagttaac tatgagataa gcaagtatca tctcatttca tttacctgaa gtcgagtaaa
     6481 cagaaaatcc aattgttgat gaacctcaat gacttagaac tatctatcgg cagatcatat
     6541 aaagaggatt taggtaccta gaggactgta cctggagtat atatatatat atatatatat
     6601 tatctcaact atagtccata gaggtttctt tcttgaggcc ttaaactgct aaagaatgat
     6661 attggtggaa tgcaagcacc aatctctctt ctttcgtaac tgttcatata cttcaaacca
     6721 agaatgtaac gggcattgac ccatccaaaa ccttcagtag ctgccccttt aaagtcagca
     6781 ccttgattac cgtattctgc ttcaacacga tgaggatctg ttcctcttgt gacatcatat
     6841 ttttcaacca caataccatt ataatcgaca aaagcctttg tcatcatgaa aagccatcta
     6901 taagctagcc tattcgttac agttaaataa ccataagaac ggaggccttc ccaagcaaga
     6961 atttgatggg gtgcccaacc aaatggatag tcccattgtc taattggtct cgaaatagaa
     7021 attgggcctc gagaacgctc cgtacatgca gctaaacctc caagcatctc taacttgggt
     7081 agtgctttct ccaccatttt ctgtgcttgc tccttcgtgg caagtccagc ccataatgcc
     7141 cagaatgtag ttgcggattc gtatgacgtt ctgtgcttga tttttgtgtt gtagtcaaag
     7201 aaaaaccccg actcgtcatc ccacatatat ttggtaattg atgaggcaac gctaattatc
     7261 aacatataga ttgttatcta tctgcatgaa cacgaaatct ttacttgacg acttgaggct
     7321 gatggtgttt atgcaaagaa accactgtgt ttaatatgtg tcactgtttg atattactgt
     7381 cagcgtagaa gataatagta aaagcggtta ataagtgtat ttgagataag tgtgataaag
     7441 tttttacagc gaaaagacga taaatacaag aaaatgatta cgaggatacg gagagaggta
     7501 tgtacatgtg tatttatata ctaagctgcc ggcggttgtt tgcaagaccg agaaaaggct
     7561 agcaagaatc gggtcattgt agcgtatgcg cctgtgaaca ttctcttcaa caagtttgat
     7621 tccattgcgg tgaaatggta aaagtcaacc ccctgcgatg tatattttcc tgtacaatca
     7681 atcaaaaagc caaatgattt agcattatct ttacatcttg ttattttaca gattttatgt
     7741 ttagatcttt tatgcttgct tttcaaaagg cctgcaggca agtgcacaaa caatacttaa
     7801 ataaatacta ctcagtaata acctatttct tagcattttt gacgaaattt gctattttgt
     7861 tagagtcttt tacaccattt gtctccacac ctccgcttac atcaacacca ataacgccat
     7921 ttaatctaag cgcatcacca acattttctg gcgtcagtcc accagctaac ataaaatgta
     7981 agctctgcct cgcgcgtttc ggtgatgacg gtgaaaacct ctgacacatg cagctcccgg
     8041 agacggtcac agcttgtctg taagcggatg ccgggagcag acaagcccgt cagggcgcgt
     8101 cagcgggtgt tggcgggtgt cggggcgcag ccatgaccca gtcacgtagc gatagcggag
     8161 tgtatactgg cttaactatg cggcatcaga gcagattgta ctgagagtgc accatatgcg
     8221 gtgtgaaata ccgcacagat gcgtaaggag aaaataccgc atcaggcgct cttccgcttc
     8281 ctcgctcact gactcgctgc gctcggtcgt tcggctgcgg cgagcggtat cagctcactc
     8341 aaaggcggta atacggttat ccacagaatc aggggataac gcaggaaaga acatgtgagc
     8401 aaaaggccag caaaaggcca ggaaccgtaa aaaggccgcg ttgctggcgt ttttccatag
     8461 gctccgcccc cctgacgagc atcacaaaaa tcgacgctca agtcagaggt ggcgaaaccc
     8521 gacaggacta taaagatacc aggcgtttcc ccctggaagc tccctcgtgc gctctcctgt
     8581 tccgaccctg ccgcttaccg gatacctgtc cgcctttctc ccttcgggaa gcgtggcgct
     8641 ttctcatagc tcacgctgta ggtatctcag ttcggtgtag gtcgttcgct ccaagctggg
     8701 ctgtgtgcac gaaccccccg ttcagcccga ccgctgcgcc ttatccggta actatcgtct
     8761 tgagtccaac ccggtaagac acgacttatc gccactggca gcagccactg gtaacaggat
     8821 tagcagagcg aggtatgtag gcggtgctac agagttcttg aagtggtggc ctaactacgg
     8881 ctacactaga aggacagtat ttggtatctg cgctctgctg aagccagtta ccttcggaaa
     8941 aagagttggt agctcttgat ccggcaaaca aaccaccgct ggtagcggtg gtttttttgt
     9001 ttgcaagcag cagattacgc gcagaaaaaa aggatctcaa gaagatcctt tgatcttttc
     9061 tacggggtct gacgctcagt ggaacgaaaa ctcacgttaa gggattttgg tcatgagatt
     9121 atcaaaaagg atcttcacct agatcctttt aaattaaaaa tgaagtttta aatcaatcta
     9181 aagtatatat gagtaaactt ggtctgacag ttaccaatgc ttaatcagtg aggcacctat
     9241 ctcagcgatc tgtctatttc gttcatccat agttgcctga ctccccgtcg tgtagataac
     9301 tacgatacgg gagggcttac catctggccc cagtgctgca atgataccgc gagacccacg
     9361 ctcaccggct ccagatttat cagcaataaa ccagccagcc ggaagggccg agcgcagaag
     9421 tggtcctgca actttatccg cctccatcca gtctattaat tgttgccggg aagctagagt
     9481 aagtagttcg ccagttaata gtttgcgcaa cgttgttgcc attgctgcag gcatcgtggt
     9541 gtcacgctcg tcgtttggta tggcttcatt cagctccggt tcccaacgat caaggcgagt
     9601 tacatgatcc cccatgttgt gcaaaaaagc ggttagctcc ttcggtcctc cgatcgttgt
     9661 cagaagtaag ttggccgcag tgttatcact catggttatg gcagcactgc ataattctct
     9721 tactgtcatg ccatccgtaa gatgcttttc tgtgactggt gagtactcaa ccaagtcatt
     9781 ctgagaatag tgtatgcggc gaccgagttg ctcttgcccg gcgtcaacac gggataatac
     9841 cgcgccacat agcagaactt taaaagtgct catcattgga aaacgttctt cggggcgaaa
     9901 actctcaagg atcttaccgc tgttgagatc cagttcgatg taacccactc gtgcacccaa
     9961 ctgatcttca gcatctttta ctttcaccag cgtttctggg tgagcaaaaa caggaaggca
    10021 aaatgccgca aaaaagggaa taagggcgac acggaaatgt tgaatactca tactcttcct
    10081 ttttcaatat tattgaagca tttatcaggg ttattgtctc atgagcggat acatatttga
    10141 atgtatttag aaaaataaac aaataggggt tccgcgcaca tttccccgaa aagtgccacc
    10201 tgacgtctaa gaaaccatta ttatcatgac attaacctat aaaaataggc gtatcacgag
    10261 gccctttcgt cttcaa
//
