LOCUS       40924_37688       10417 bp DNA     circular SYN 18-DEC-2018
DEFINITION  Cloning vector pRR24, complete sequence.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 10417)
  AUTHORS   Ramsden R, Arms L, Davis TN, Muller EG.
  TITLE     An intein with genetically selectable markers provides a new 
            approach to internally label proteins with GFP
  JOURNAL   BMC Biotechnol. 11 (1), 71 (2011)
  PUBMED    21708017
REFERENCE   2  (bases 1 to 10417)
  AUTHORS   Ramsden R, Arms L, Davis TN, Muller EGD.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-JUN-2011) Biochemistry, University of Washington, 1705
            NE Pacific St, HSB J461, Seattle, WA 98195, USA
REFERENCE   3  (bases 1 to 10417)
  TITLE     Direct Submission
REFERENCE   4  (bases 1 to 10417)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "BMC 
            Biotechnol."; date: "2011"; volume: "11"; issue: "1"; pages: "71"
COMMENT     SGRef: number: 2; type: "Journal Article"; journalName: "Submitted 
            (30-JUN-2011) Biochemistry, University of Washington, 1705 NE 
            Pacific St, HSB J461, Seattle, WA 98195, USA"
COMMENT     SGRef: number: 3; type: "Journal Article"
FEATURES             Location/Qualifiers
     source          1..10417
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        14..678
                     /label=GAL1,10 promoter
                     /note="divergent inducible promoter, regulated by Gal4"
     CDS             694..714
                     /codon_start=1
                     /label=SV40 NLS
                     /note="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /translation="PKKKRKV"
     CDS             724..744
                     /codon_start=1
                     /label=SV40 NLS
                     /note="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /translation="PKKKRKV"
     CDS             748..768
                     /codon_start=1
                     /label=SV40 NLS
                     /note="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /translation="PKKKRKV"
     CDS             781..1491
                     /codon_start=1
                     /label=GFP (S65T)
                     /note="S65T variant of Aequorea victoria green fluorescent 
                     protein (Heim et al., 1995)"
                     /translation="SKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTLKF
                     ICTTGKLPVPWPTLVTTFTYGVQCFSRYPDHMKRHDFFKSAMPEGYVQERTIFFKDDGN
                     YKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIKVN
                     FKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLLEF
                     VTAAGITHGMDELYK"
     CDS             1909..2928
                     /codon_start=1
                     /label=HygR
                     /note="aminoglycoside phosphotransferase from E. coli"
                     /translation="KKPELTATSVEKFLIEKFDSVSDLMQLSEGEESRAFSFDVGGRGY
                     VLRVNSCADGFYKDRYVYRHFASAALPIPEVLDIGEFSESLTYCISRRAQGVTLQDLPE
                     TELPAVLQPVAEAMDAIAAADLSQTSGFGPFGPQGIGQYTTWRDFICAIADPHVYHWQT
                     VMDDTVSASVAQALDELMLWAEDCPEVRHLVHADFGSNNVLTDNGRITAVIDWSEAMFG
                     DSQYEVANIFFWRPWLACMEQQTRYFERRHPELAGSPRLRAYMLRIGLDQLYQSLVDGN
                     FDDAAWAQGRCDAIVRSGAGTVGRTQIARRSAAVWTDGCVEVLADSGNRRPSTRPRAKE
                     "
     CDS             3094..3117
                     /codon_start=1
                     /label=FLAG
                     /note="FLAG(R) epitope tag, followed by an enterokinase
                     cleavage site"
                     /translation="DYKDDDDK"
     promoter        3907..4127
                     /label=URA3 promoter
     CDS             4128..4928
                     /codon_start=1
                     /label=URA3
                     /note="orotidine-5'-phosphate decarboxylase, required for
                     uracil biosynthesis"
                     /translation="MSKATYKERAATHPSPVAAKLFNIMHEKQTNLCASLDVRTTKELL
                     ELVEALGPKICLLKTHVDILTDFSMEGTVKPLKALSAKYNFLLFEDRKFADIGNTVKLQ
                     YSAGVYRIAEWADITNAHGVVGPGIVSGLKQAAEEVTKEPRGLLMLAELSCKGSLSTGE
                     YTKGTVDIAKSDKDFVIGFIAQRDMGGRDEGYDWLIMTPGVGLDDKGDALGQQYRTVDD
                     VVSTGSDIIIVGRGLFAKGRDAKVEGERYRKAGWEAYLRRCGQQN"
     misc_feature    complement(6276..8125)
                     /label=CEN/ARS
                     /note="S. cerevisiae CEN4 centromere fused to the
                     autonomously replicating sequence ARS1/ARS416"
     misc_feature    8267..8407
                     /label=bom
                     /note="basis of mobility region from pBR322"
     rep_origin      complement(8593..9181)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     CDS             complement(9355..10212)
                     /codon_start=1
                     /label=AmpR
                     /note="beta-lactamase"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRVDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPAAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     promoter        complement(10213..10317)
                     /label=AmpR promoter
ORIGIN
        1 gaattccttg aattttcaaa aattcttact ttttttttgg atggacgcaa agaagtttaa
       61 taatcatatt acatggcatt accaccatat acatatccat atacatatcc atatctaatc
      121 ttacttatat gttgtggaaa tgtaaagagc cccattatct tagcctaaaa aaaccttctc
      181 tttggaactt tcagtaatac gcttaactgc tcattgctat attgaagtac ggattagaag
      241 ccgccgagcg ggtgacagcc ctccgaagga agactctcct ccgtgcgtcc tcgtcttcac
      301 cggtcgcgtt cctgaaacgc agatgtgcct cgcgccgcac tgctccgaac aataaagatt
      361 ctacaatact agcttttatg gttatgaaga ggaaaaattg gcagtaacct ggccccacaa
      421 accttcaaat gaacgaatca aattaacaac cataggatga taatgcgatt agttttttag
      481 ccttatttct ggggtaatta atcagcgaag cgatgatttt tgatctatta acagatatat
      541 aaatgcaaaa actgcataac cactttaact aatactttca acattttcgg tttgtattac
      601 ttcttattca aatgtaataa aagtatcaac aaaaaattgt taatatacct ctatacttta
      661 acgtcaagga gaaaaaaccc cggatccatg gttccaaaga agaagagaaa ggttttaatt
      721 gatccaaaaa agaaaagaaa ggttgatcca aagaaaaaga gaaaagtttt aattaaagga
      781 agtaaaggag aagaactttt cactggagtt gtcccaattc ttgttgaatt agatggtgat
      841 gttaatgggc acaaattttc tgtcagtgga gagggtgaag gtgatgcaac atacggaaaa
      901 cttaccctta aatttatttg cactactgga aaactacctg ttccatggcc aacacttgtc
      961 actactttca cttatggtgt tcaatgcttt tcaagatacc cagatcatat gaaacggcat
     1021 gactttttca agagtgccat gcccgaaggt tatgtacagg aaagaactat atttttcaaa
     1081 gatgacggga actacaagac acgtgctgaa gtcaagtttg aaggtgatac ccttgttaat
     1141 agaatcgagt taaaaggtat tgattttaaa gaagatggaa acattcttgg acacaaattg
     1201 gaatacaact ataactcaca caatgtatac atcatggcag acaaacaaaa gaatggaatc
     1261 aaagttaact tcaaaattag acacaacatt gaagatggaa gcgttcaact agcagaccat
     1321 tatcaacaaa atactccaat tggcgatggc cctgtccttt taccagacaa ccattacctg
     1381 tccacacaat ctgccctttc gaaagatccc aacgaaaaga gagaccacat ggtccttctt
     1441 gagtttgtaa cagctgctgg gattacacat ggcatggatg aactatacaa aggtgctggt
     1501 gcaggtgcag gagctggagc aggtttctgg gagaaagcgt gtttggccaa gggaacccgt
     1561 ttgttgagat gcgatggaac cgagatcaat gtggaagacg tgcgcgaagg tgacctactt
     1621 ctgggtcccg atggagagcc tcgccgtgca ttcaacatag tgaatggcat cgacagactg
     1681 tacagaatca agatcggcgg tgagaaagag gaccttgtgg tgacgccgaa ccatattctg
     1741 gtgctttata gagaggatgg ttccaagaat gtggagaagc aaacggtgga gatcactgct
     1801 gccgagtttg ccgcgctttc taccgaggaa agaagcctct atagtgcctt tacatctcct
     1861 agggctgaga agggcgccgc aggtgcaggt gcaggtgcag ctagcggtaa aaagcctgaa
     1921 ctcaccgcga cgtctgtcga gaagtttctg atcgaaaagt tcgacagcgt ctccgacctg
     1981 atgcagctct cggagggcga agaatctcgt gctttcagct tcgatgtagg agggcgtgga
     2041 tatgtcctgc gggtaaatag ctgcgccgat ggtttctaca aagatcgtta tgtttatcgg
     2101 cactttgcat cggccgcgct cccgattccg gaagtgcttg acattgggga attcagcgag
     2161 agcctgacct attgcatctc ccgccgtgca cagggtgtca cgttgcaaga cctgcctgaa
     2221 accgaactgc ccgctgttct gcagccggtc gcggaggcca tggatgcgat cgctgcggcc
     2281 gatcttagcc agacgagcgg gttcggccca ttcggaccgc aaggaatcgg tcaatacact
     2341 acatggcgtg atttcatatg cgcgattgct gatccccatg tgtatcactg gcaaactgtg
     2401 atggacgaca ccgtcagtgc gtccgtcgcg caggctctcg atgagctgat gctttgggcc
     2461 gaggactgcc ccgaagtccg gcacctcgtg cacgcggatt tcggctccaa caatgtcctg
     2521 acggacaatg gccgcataac agcggtcatt gactggagcg aggcgatgtt cggggattcc
     2581 caatacgagg tcgccaacat cttcttctgg aggccgtggt tggcttgtat ggagcagcag
     2641 acgcgctact tcgagcggag gcatccggag cttgcaggat cgccgcggct ccgggcgtat
     2701 atgctccgca ttggtcttga ccaactctat cagagcttgg ttgacggcaa tttcgatgat
     2761 gcagcttggg cgcagggtcg atgcgacgca atcgtccgat ccggagccgg gactgtcggg
     2821 cgtacacaaa tcgcccgcag aagcgcggcc gtctggaccg atggctgtgt agaagtactc
     2881 gccgatagtg gaaaccgacg ccccagcact cgtccgaggg caaaggaagg taccgcgggt
     2941 gctggtgctg gtgcttcggc tcaaacgcac agtttcaaga ttgagcaagt tagcctcgaa
     3001 tccgagaaga cagagtgggc tggtttccga gtcgacaaag atcagcttta cctgcgtcat
     3061 gactaccttg tcctgcacaa cagtggtttc gaagattaca aggatgacga cgataagtaa
     3121 gtcgaccgat gcccttgaga gccttcaacc cagtcagctc cttccggtgg gcgcggggca
     3181 tgactatcgt cgccgcactt atgactgtct tctttatcat gcaactcgta ggacaggtgc
     3241 cggcagcgct ctgggtcatt ttcggcgagg accgctttcg ctggagcgcg acgatgatcg
     3301 gcctgtcgct tgcggtattc ggaatcttgc acgccctcgc tcaagccttc gtcactggtc
     3361 ccgccaccaa acgtttcggc gagaagcagg ccattatcgc cggcatggcg gccgacgcgc
     3421 tgggctacgt cttgctggcg ttcgcgacgc gaggctggat ggccttcccc attatgattc
     3481 ttctcgcttc cggcggcatc gggatgcccg cgttgcaggc catgctgtcc aggcaggtag
     3541 atgacgacca tcagggacag cttcaaggat cgctcgcggc tcttaccagc ctaacttcga
     3601 tcactggacc gctgatcgtc acggcgattt atgccgcctc ggcgagcaca tggaacgggt
     3661 tggcatggat tgtaggcgcc gccctatacc ttgtctgcct ccccgcgttg cgtcgcggtg
     3721 catggagccg ggccacctcg acctgaatgg aagccggcgg cacctcgcta acggattcac
     3781 cactccaaga attggagcca atcaattctt gcggagaact gtgaatgcgc aaaccaaccc
     3841 ttggcagaac atatccatcg cgtccgccat ctccagcagc cgcacgcggc gcatcggggg
     3901 gggggtttca attcaattca tcattttttt tttattcttt tttttgattt cggtttcttt
     3961 gaaatttttt tgattcggta atctccgaac agaaggaaga acgaaggaag gagcacagac
     4021 ttagattggt atatatacgc atatgtagtg ttgaagaaac atgaaattgc ccagtattct
     4081 taacccaact gcacagaaca aaaacctgca ggaaacgaag ataaatcatg tcgaaagcta
     4141 catataagga acgtgctgct actcatccta gtcctgttgc tgccaagcta tttaatatca
     4201 tgcacgaaaa gcaaacaaac ttgtgtgctt cattggatgt tcgtaccacc aaggaattac
     4261 tggagttagt tgaagcatta ggtcccaaaa tttgtttact aaaaacacat gtggatatct
     4321 tgactgattt ttccatggag ggcacagtta agccgctaaa ggcattatcc gccaagtaca
     4381 attttttact cttcgaagac agaaaatttg ctgacattgg taatacagtc aaattgcagt
     4441 actctgcggg tgtatacaga atagcagaat gggcagacat tacgaatgca cacggtgtgg
     4501 tgggcccagg tattgttagc ggtttgaagc aggcggcaga agaagtaaca aaggaaccta
     4561 gaggcctttt gatgttagca gaattgtcat gcaagggctc cctatctact ggagaatata
     4621 ctaagggtac tgttgacatt gcgaagagcg acaaagattt tgttatcggc tttattgctc
     4681 aaagagacat gggtggaaga gatgaaggtt acgattggtt gattatgaca cccggtgtgg
     4741 gtttagatga caagggagac gcattgggtc aacagtatag aaccgtggat gatgtggtct
     4801 ctacaggatc tgacattatt attgttggaa gaggactatt tgcaaaggga agggatgcta
     4861 aggtagaggg tgaacgttac agaaaagcag gctgggaagc atatttgaga agatgcggcc
     4921 agcaaaacta aaaaactgta ttataagtaa atgcatgtat actaaactca caaattagag
     4981 cttcaattta attatatcag ttattacccg ggaatctcgg tcgtaatgat ttttataatg
     5041 acgaaaaaaa aaaaattgga aagaaaaccc cccccccgca gcgttgggtc ctggccacgg
     5101 gtgcgcatga tcgtgctcct gtcgttgagg acccggctag gctggcgggg ttgccttact
     5161 ggttagcaga atgaatcacc gatacgcgag cgaacgtgaa gcgactgctg ctgcaaaacg
     5221 tctgcgacct gagcaacaac atgaatggtc ttcggtttcc gtgtttcgta aagtctggaa
     5281 acgcggaagt cagcgccctg caccattatg ttccggatct gcatcgcagg atgctgctgg
     5341 ctaccctgtg gaacacctac atctgtatta acgaagcgct ggcattgacc ctgagtgatt
     5401 tttctctggt cccgccgcat ccataccgcc agttgtttac cctcacaacg ttccagtaac
     5461 cgggcatgtt catcatcagt aacccgtatc gtgagcatcc tctctcgttt catcggtatc
     5521 attaccccca tgaacagaaa ttccccctta cacggaggca tcaagtgacc aaacaggaaa
     5581 aaaccgccct taacatggcc cgctttatca gaagccagac attaacgctt ctggagaaac
     5641 tcaacgagct ggacgcggat gaacaggcag acatctgtga atcgcttcac gaccacgctg
     5701 atgagcttta ccgcaggtgg gccattctca tgaagaatat cttgaattta ttgtcatatt
     5761 actagttggt gtggaagtcc atatatcggt gatcaatata gtggttgaca tgctggctag
     5821 tcaacattga gccttttgat catgcaaata tattacggta ttttacaatc aaatatcaaa
     5881 cttaactatt gactttataa cttatttagg tggtaacatt cttataaaaa agaaaaaaat
     5941 tactgcaaaa cagtactagc ttttaacttg tatcctaggt tatctatgct gtctcaccat
     6001 agagaatatt acctatttca gaatgtatgt ccatgattcg ccgggtaaat acatataata
     6061 cacaaatctg gcttaataaa gtctataata tatctcataa agaagtgcta aattggctag
     6121 tgctatatat ttttaagaaa atttcttttg actaagtcca tatcgacttt gtaaaagttc
     6181 actttagcat acatatatta cacgagccag aaattgtaac ttttgcctaa aatcacaaat
     6241 tgcaaaattt aattgcttgc aaaaggtcac atgcttataa tcaacttttt taaaaattta
     6301 aaatactttt ttatttttta tttttaaaca taaatgaaat aatttattta ttgtttatga
     6361 ttaccgaaac ataaaacctg ctcaagaaaa agaaactgtt ttgtccttgg aaaaaaagca
     6421 ctacctagga gcggccaaaa tgccgaggct ttcatagctt aaactcttta cagaaaatag
     6481 gcattataga tcagttcgag ttttcttatt cttccttccg gttttatcgt cacagtttta
     6541 cagtaaataa gtatcacctc ttagagttaa ctatgagata agcaagtatc atctcatttc
     6601 atttacctga agtcgagtaa acagaaaatc caattgttga tgaacctcaa tgacttagaa
     6661 ctatctatcg gcagatcata taaagaggat ttaggtacct agaggactgt acctggagta
     6721 tatatatata tatatatata ttatctcaac tatagtccat agaggtttct ttcttgaggc
     6781 cttaaactgc taaagaatga tattggtgga atgcaagcac caatctctct tctttcgtaa
     6841 ctgttcatat acttcaaacc aagaatgtaa cgggcattga cccatccaaa accttcagta
     6901 gctgcccctt taaagtcagc accttgatta ccgtattctg cttcaacacg atgaggatct
     6961 gttcctcttg tgacatcata tttttcaacc acaataccat tataatcgac aaaagccttt
     7021 gtcatcatga aaagccatct ataagctagc ctattcgtta cagttaaata accataagaa
     7081 cggaggcctt cccaagcaag aatttgatgg ggtgcccaac caaatggata gtcccattgt
     7141 ctaattggtc tcgaaataga aattgggcct cgagaacgct ccgtacatgc agctaaacct
     7201 ccaagcatct ctaacttggg tagtgctttc tccaccattt tctgtgcttg ctccttcgtg
     7261 gcaagtccag cccataatgc ccagaatgta gttgcggatt cgtatgacgt tctgtgcttg
     7321 atttttgtgt tgtagtcaaa gaaaaacccc gactcgtcat cccacatata tttggtaatt
     7381 gatgaggcaa cgctaattat caacatatag attgttatct atctgcatga acacgaaatc
     7441 tttacttgac gacttgaggc tgatggtgtt tatgcaaaga aaccactgtg tttaatatgt
     7501 gtcactgttt gatattactg tcagcgtaga agataatagt aaaagcggtt aataagtgta
     7561 tttgagataa gtgtgataaa gtttttacag cgaaaagacg ataaatacaa gaaaatgatt
     7621 acgaggatac ggagagaggt atgtacatgt gtatttatat actaagctgc cggcggttgt
     7681 ttgcaagacc gagaaaaggc tagcaagaat cgggtcattg tagcgtatgc gcctgtgaac
     7741 attctcttca acaagtttga ttccattgcg gtgaaatggt aaaagtcaac cccctgcgat
     7801 gtatattttc ctgtacaatc aatcaaaaag ccaaatgatt tagcattatc tttacatctt
     7861 gttattttac agattttatg tttagatctt ttatgcttgc ttttcaaaag gcctgcaggc
     7921 aagtgcacaa acaatactta aataaatact actcagtaat aacctatttc ttagcatttt
     7981 tgacgaaatt tgctattttg ttagagtctt ttacaccatt tgtctccaca cctccgctta
     8041 catcaacacc aataacgcca tttaatctaa gcgcatcacc aacattttct ggcgtcagtc
     8101 caccagctaa cataaaatgt aagctctgcc tcgcgcgttt cggtgatgac ggtgaaaacc
     8161 tctgacacat gcagctcccg gagacggtca cagcttgtct gtaagcggat gccgggagca
     8221 gacaagcccg tcagggcgcg tcagcgggtg ttggcgggtg tcggggcgca gccatgaccc
     8281 agtcacgtag cgatagcgga gtgtatactg gcttaactat gcggcatcag agcagattgt
     8341 actgagagtg caccatatgc ggtgtgaaat accgcacaga tgcgtaagga gaaaataccg
     8401 catcaggcgc tcttccgctt cctcgctcac tgactcgctg cgctcggtcg ttcggctgcg
     8461 gcgagcggta tcagctcact caaaggcggt aatacggtta tccacagaat caggggataa
     8521 cgcaggaaag aacatgtgag caaaaggcca gcaaaaggcc aggaaccgta aaaaggccgc
     8581 gttgctggcg tttttccata ggctccgccc ccctgacgag catcacaaaa atcgacgctc
     8641 aagtcagagg tggcgaaacc cgacaggact ataaagatac caggcgtttc cccctggaag
     8701 ctccctcgtg cgctctcctg ttccgaccct gccgcttacc ggatacctgt ccgcctttct
     8761 cccttcggga agcgtggcgc tttctcatag ctcacgctgt aggtatctca gttcggtgta
     8821 ggtcgttcgc tccaagctgg gctgtgtgca cgaacccccc gttcagcccg accgctgcgc
     8881 cttatccggt aactatcgtc ttgagtccaa cccggtaaga cacgacttat cgccactggc
     8941 agcagccact ggtaacagga ttagcagagc gaggtatgta ggcggtgcta cagagttctt
     9001 gaagtggtgg cctaactacg gctacactag aaggacagta tttggtatct gcgctctgct
     9061 gaagccagtt accttcggaa aaagagttgg tagctcttga tccggcaaac aaaccaccgc
     9121 tggtagcggt ggtttttttg tttgcaagca gcagattacg cgcagaaaaa aaggatctca
     9181 agaagatcct ttgatctttt ctacggggtc tgacgctcag tggaacgaaa actcacgtta
     9241 agggattttg gtcatgagat tatcaaaaag gatcttcacc tagatccttt taaattaaaa
     9301 atgaagtttt aaatcaatct aaagtatata tgagtaaact tggtctgaca gttaccaatg
     9361 cttaatcagt gaggcaccta tctcagcgat ctgtctattt cgttcatcca tagttgcctg
     9421 actccccgtc gtgtagataa ctacgatacg ggagggctta ccatctggcc ccagtgctgc
     9481 aatgataccg cgagacccac gctcaccggc tccagattta tcagcaataa accagccagc
     9541 cggaagggcc gagcgcagaa gtggtcctgc aactttatcc gcctccatcc agtctattaa
     9601 ttgttgccgg gaagctagag taagtagttc gccagttaat agtttgcgca acgttgttgc
     9661 cattgctgca ggcatcgtgg tgtcacgctc gtcgtttggt atggcttcat tcagctccgg
     9721 ttcccaacga tcaaggcgag ttacatgatc ccccatgttg tgcaaaaaag cggttagctc
     9781 cttcggtcct ccgatcgttg tcagaagtaa gttggccgca gtgttatcac tcatggttat
     9841 ggcagcactg cataattctc ttactgtcat gccatccgta agatgctttt ctgtgactgg
     9901 tgagtactca accaagtcat tctgagaata gtgtatgcgg cgaccgagtt gctcttgccc
     9961 ggcgtcaaca cgggataata ccgcgccaca tagcagaact ttaaaagtgc tcatcattgg
    10021 aaaacgttct tcggggcgaa aactctcaag gatcttaccg ctgttgagat ccagttcgat
    10081 gtaacccact cgtgcaccca actgatcttc agcatctttt actttcacca gcgtttctgg
    10141 gtgagcaaaa acaggaaggc aaaatgccgc aaaaaaggga ataagggcga cacggaaatg
    10201 ttgaatactc atactcttcc tttttcaata ttattgaagc atttatcagg gttattgtct
    10261 catgagcgga tacatatttg aatgtattta gaaaaataaa caaatagggg ttccgcgcac
    10321 atttccccga aaagtgccac ctgacgtcta agaaaccatt attatcatga cattaaccta
    10381 taaaaatagg cgtatcacga ggccctttcg tcttcaa
//
