LOCUS       40924_37683       10042 bp DNA     circular SYN 18-DEC-2018
DEFINITION  Cloning vector pRR23, complete sequence.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 10042)
  AUTHORS   Ramsden R, Arms L, Davis TN, Muller EG.
  TITLE     An intein with genetically selectable markers provides a new 
            approach to internally label proteins with GFP
  JOURNAL   BMC Biotechnol. 11 (1), 71 (2011)
  PUBMED    21708017
REFERENCE   2  (bases 1 to 10042)
  AUTHORS   Ramsden R, Arms L, Davis TN, Muller EGD.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-JUN-2011) Biochemistry, University of Washington, 1705
            NE Pacific St, HSB J461, Seattle, WA 98195, USA
REFERENCE   3  (bases 1 to 10042)
  TITLE     Direct Submission
REFERENCE   4  (bases 1 to 10042)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "BMC 
            Biotechnol."; date: "2011"; volume: "11"; issue: "1"; pages: "71"
COMMENT     SGRef: number: 2; type: "Journal Article"; journalName: "Submitted 
            (30-JUN-2011) Biochemistry, University of Washington, 1705 NE 
            Pacific St, HSB J461, Seattle, WA 98195, USA"
COMMENT     SGRef: number: 3; type: "Journal Article"
FEATURES             Location/Qualifiers
     source          1..10042
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        14..678
                     /label=GAL1,10 promoter
                     /note="divergent inducible promoter, regulated by Gal4"
     CDS             694..714
                     /codon_start=1
                     /label=SV40 NLS
                     /note="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /translation="PKKKRKV"
     CDS             724..744
                     /codon_start=1
                     /label=SV40 NLS
                     /note="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /translation="PKKKRKV"
     CDS             748..768
                     /codon_start=1
                     /label=SV40 NLS
                     /note="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /translation="PKKKRKV"
     CDS             781..1491
                     /codon_start=1
                     /label=GFP (S65T)
                     /note="S65T variant of Aequorea victoria green fluorescent 
                     protein (Heim et al., 1995)"
                     /translation="SKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTLKF
                     ICTTGKLPVPWPTLVTTFTYGVQCFSRYPDHMKRHDFFKSAMPEGYVQERTIFFKDDGN
                     YKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIKVN
                     FKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLLEF
                     VTAAGITHGMDELYK"
     misc_feature    1540..2706
                     /label=Pch PRP8 intein with SpHis5
                     /note="Pch PRP8 intein with SpHis5"
     CDS             1906..2553
                     /codon_start=1
                     /gene="Schizosaccharomyces pombe his5"
                     /product="imidazoleglycerol-phosphate dehydratase, required
                     for histidine biosynthesis"
                     /label=S. pombe his5
                     /note="yeast auxotrophic marker; corresponds to S.
                     cerevisiae HIS3"
                     /translation="GRRAFVERNTNETKISVAIALDKAPLPEESNFIDELITSKHANQK
                     GEQVIQVDTGIGFLDHMYHALAKHAGWSLRLYSRGDLIIDDHHTAEDTAIALGIAFKQA
                     MGNFAGVKRFGHAYCPLDEALSRSVVDLSGRPYAVIDLGLKREKVGELSCEMIPHLLYS
                     FSVAAGITLHVTCLYGSNDHHRAESAFKSLAVAMRAATSLTGSSEVPSTKGVL"
     CDS             2719..2742
                     /codon_start=1
                     /label=FLAG
                     /note="FLAG(R) epitope tag, followed by an enterokinase
                     cleavage site"
                     /translation="DYKDDDDK"
     promoter        3532..3752
                     /label=URA3 promoter
     CDS             3753..4553
                     /codon_start=1
                     /label=URA3
                     /note="orotidine-5'-phosphate decarboxylase, required for
                     uracil biosynthesis"
                     /translation="MSKATYKERAATHPSPVAAKLFNIMHEKQTNLCASLDVRTTKELL
                     ELVEALGPKICLLKTHVDILTDFSMEGTVKPLKALSAKYNFLLFEDRKFADIGNTVKLQ
                     YSAGVYRIAEWADITNAHGVVGPGIVSGLKQAAEEVTKEPRGLLMLAELSCKGSLSTGE
                     YTKGTVDIAKSDKDFVIGFIAQRDMGGRDEGYDWLIMTPGVGLDDKGDALGQQYRTVDD
                     VVSTGSDIIIVGRGLFAKGRDAKVEGERYRKAGWEAYLRRCGQQN"
     misc_feature    complement(5901..7750)
                     /label=CEN/ARS
                     /note="S. cerevisiae CEN4 centromere fused to the
                     autonomously replicating sequence ARS1/ARS416"
     misc_feature    7892..8032
                     /label=bom
                     /note="basis of mobility region from pBR322"
     rep_origin      complement(8218..8806)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     CDS             complement(8980..9837)
                     /codon_start=1
                     /label=AmpR
                     /note="beta-lactamase"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRVDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPAAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     promoter        complement(9838..9942)
                     /label=AmpR promoter
ORIGIN
        1 gaattccttg aattttcaaa aattcttact ttttttttgg atggacgcaa agaagtttaa
       61 taatcatatt acatggcatt accaccatat acatatccat atacatatcc atatctaatc
      121 ttacttatat gttgtggaaa tgtaaagagc cccattatct tagcctaaaa aaaccttctc
      181 tttggaactt tcagtaatac gcttaactgc tcattgctat attgaagtac ggattagaag
      241 ccgccgagcg ggtgacagcc ctccgaagga agactctcct ccgtgcgtcc tcgtcttcac
      301 cggtcgcgtt cctgaaacgc agatgtgcct cgcgccgcac tgctccgaac aataaagatt
      361 ctacaatact agcttttatg gttatgaaga ggaaaaattg gcagtaacct ggccccacaa
      421 accttcaaat gaacgaatca aattaacaac cataggatga taatgcgatt agttttttag
      481 ccttatttct ggggtaatta atcagcgaag cgatgatttt tgatctatta acagatatat
      541 aaatgcaaaa actgcataac cactttaact aatactttca acattttcgg tttgtattac
      601 ttcttattca aatgtaataa aagtatcaac aaaaaattgt taatatacct ctatacttta
      661 acgtcaagga gaaaaaaccc cggatccatg gttccaaaga agaagagaaa ggttttaatt
      721 gatccaaaaa agaaaagaaa ggttgatcca aagaaaaaga gaaaagtttt aattaaagga
      781 agtaaaggag aagaactttt cactggagtt gtcccaattc ttgttgaatt agatggtgat
      841 gttaatgggc acaaattttc tgtcagtgga gagggtgaag gtgatgcaac atacggaaaa
      901 cttaccctta aatttatttg cactactgga aaactacctg ttccatggcc aacacttgtc
      961 actactttca cttatggtgt tcaatgcttt tcaagatacc cagatcatat gaaacggcat
     1021 gactttttca agagtgccat gcccgaaggt tatgtacagg aaagaactat atttttcaaa
     1081 gatgacggga actacaagac acgtgctgaa gtcaagtttg aaggtgatac ccttgttaat
     1141 agaatcgagt taaaaggtat tgattttaaa gaagatggaa acattcttgg acacaaattg
     1201 gaatacaact ataactcaca caatgtatac atcatggcag acaaacaaaa gaatggaatc
     1261 aaagttaact tcaaaattag acacaacatt gaagatggaa gcgttcaact agcagaccat
     1321 tatcaacaaa atactccaat tggcgatggc cctgtccttt taccagacaa ccattacctg
     1381 tccacacaat ctgccctttc gaaagatccc aacgaaaaga gagaccacat ggtccttctt
     1441 gagtttgtaa cagctgctgg gattacacat ggcatggatg aactatacaa aggtgctggt
     1501 gcaggtgcag gagctggagc aggtttctgg gagaaagcgt gtttggccaa gggaacccgt
     1561 ttgttgagat gcgatggaac cgagatcaat gtggaagacg tgcgcgaagg tgacctactt
     1621 ctgggtcccg atggagagcc tcgccgtgca ttcaacatag tgaatggcat cgacagactg
     1681 tacagaatca agatcggcgg tgagaaagag gaccttgtgg tgacgccgaa ccatattctg
     1741 gtgctttata gagaggatgg ttccaagaat gtggagaagc aaacggtgga gatcactgct
     1801 gccgagtttg ccgcgctttc taccgaggaa agaagcctct atagtgcctt tacatctcct
     1861 agggctgaga agggcgccgc aggtgcaggt gcaggtgcag ctagcggtag gagggctttt
     1921 gtagaaagaa atacgaacga aacgaaaatc agcgttgcca tcgctttgga caaagctccc
     1981 ttacctgaag agtcgaattt tattgatgaa cttataactt ccaagcatgc aaaccaaaag
     2041 ggagaacaag taatccaagt agacacggga attggattct tggatcacat gtatcatgca
     2101 ctggctaaac atgcaggctg gagcttacga ctttactcaa gaggtgattt aatcatcgat
     2161 gatcatcaca ctgcagaaga tactgctatt gcacttggta ttgcattcaa gcaggctatg
     2221 ggtaactttg ccggcgttaa aagatttgga catgcttatt gtccacttga cgaagctctt
     2281 tctagaagcg tagttgactt gtcgggacgg ccctatgctg ttatcgattt gggattaaag
     2341 cgtgaaaagg ttggggaatt gtcctgtgaa atgatccctc acttactata ttccttttcg
     2401 gtagcagctg gaattacttt gcatgttacc tgcttatatg gtagtaatga ccatcatcgt
     2461 gctgaaagcg cttttaaatc tctggctgtt gccatgcgcg cggctactag tcttactgga
     2521 agttctgaag tcccaagcac gaagggagtg ttgggtaccg cgggtgctgg tgctggtgct
     2581 tcggctcaaa cgcacagttt caagattgag caagttagcc tcgaatccga gaagacagag
     2641 tgggctggtt tccgagtcga caaagatcag ctttacctgc gtcatgacta ccttgtcctg
     2701 cacaacagtg gtttcgaaga ttacaaggat gacgacgata agtaagtcga ccgatgccct
     2761 tgagagcctt caacccagtc agctccttcc ggtgggcgcg gggcatgact atcgtcgccg
     2821 cacttatgac tgtcttcttt atcatgcaac tcgtaggaca ggtgccggca gcgctctggg
     2881 tcattttcgg cgaggaccgc tttcgctgga gcgcgacgat gatcggcctg tcgcttgcgg
     2941 tattcggaat cttgcacgcc ctcgctcaag ccttcgtcac tggtcccgcc accaaacgtt
     3001 tcggcgagaa gcaggccatt atcgccggca tggcggccga cgcgctgggc tacgtcttgc
     3061 tggcgttcgc gacgcgaggc tggatggcct tccccattat gattcttctc gcttccggcg
     3121 gcatcgggat gcccgcgttg caggccatgc tgtccaggca ggtagatgac gaccatcagg
     3181 gacagcttca aggatcgctc gcggctctta ccagcctaac ttcgatcact ggaccgctga
     3241 tcgtcacggc gatttatgcc gcctcggcga gcacatggaa cgggttggca tggattgtag
     3301 gcgccgccct ataccttgtc tgcctccccg cgttgcgtcg cggtgcatgg agccgggcca
     3361 cctcgacctg aatggaagcc ggcggcacct cgctaacgga ttcaccactc caagaattgg
     3421 agccaatcaa ttcttgcgga gaactgtgaa tgcgcaaacc aacccttggc agaacatatc
     3481 catcgcgtcc gccatctcca gcagccgcac gcggcgcatc gggggggggg tttcaattca
     3541 attcatcatt ttttttttat tctttttttt gatttcggtt tctttgaaat ttttttgatt
     3601 cggtaatctc cgaacagaag gaagaacgaa ggaaggagca cagacttaga ttggtatata
     3661 tacgcatatg tagtgttgaa gaaacatgaa attgcccagt attcttaacc caactgcaca
     3721 gaacaaaaac ctgcaggaaa cgaagataaa tcatgtcgaa agctacatat aaggaacgtg
     3781 ctgctactca tcctagtcct gttgctgcca agctatttaa tatcatgcac gaaaagcaaa
     3841 caaacttgtg tgcttcattg gatgttcgta ccaccaagga attactggag ttagttgaag
     3901 cattaggtcc caaaatttgt ttactaaaaa cacatgtgga tatcttgact gatttttcca
     3961 tggagggcac agttaagccg ctaaaggcat tatccgccaa gtacaatttt ttactcttcg
     4021 aagacagaaa atttgctgac attggtaata cagtcaaatt gcagtactct gcgggtgtat
     4081 acagaatagc agaatgggca gacattacga atgcacacgg tgtggtgggc ccaggtattg
     4141 ttagcggttt gaagcaggcg gcagaagaag taacaaagga acctagaggc cttttgatgt
     4201 tagcagaatt gtcatgcaag ggctccctat ctactggaga atatactaag ggtactgttg
     4261 acattgcgaa gagcgacaaa gattttgtta tcggctttat tgctcaaaga gacatgggtg
     4321 gaagagatga aggttacgat tggttgatta tgacacccgg tgtgggttta gatgacaagg
     4381 gagacgcatt gggtcaacag tatagaaccg tggatgatgt ggtctctaca ggatctgaca
     4441 ttattattgt tggaagagga ctatttgcaa agggaaggga tgctaaggta gagggtgaac
     4501 gttacagaaa agcaggctgg gaagcatatt tgagaagatg cggccagcaa aactaaaaaa
     4561 ctgtattata agtaaatgca tgtatactaa actcacaaat tagagcttca atttaattat
     4621 atcagttatt acccgggaat ctcggtcgta atgattttta taatgacgaa aaaaaaaaaa
     4681 ttggaaagaa aacccccccc ccgcagcgtt gggtcctggc cacgggtgcg catgatcgtg
     4741 ctcctgtcgt tgaggacccg gctaggctgg cggggttgcc ttactggtta gcagaatgaa
     4801 tcaccgatac gcgagcgaac gtgaagcgac tgctgctgca aaacgtctgc gacctgagca
     4861 acaacatgaa tggtcttcgg tttccgtgtt tcgtaaagtc tggaaacgcg gaagtcagcg
     4921 ccctgcacca ttatgttccg gatctgcatc gcaggatgct gctggctacc ctgtggaaca
     4981 cctacatctg tattaacgaa gcgctggcat tgaccctgag tgatttttct ctggtcccgc
     5041 cgcatccata ccgccagttg tttaccctca caacgttcca gtaaccgggc atgttcatca
     5101 tcagtaaccc gtatcgtgag catcctctct cgtttcatcg gtatcattac ccccatgaac
     5161 agaaattccc ccttacacgg aggcatcaag tgaccaaaca ggaaaaaacc gcccttaaca
     5221 tggcccgctt tatcagaagc cagacattaa cgcttctgga gaaactcaac gagctggacg
     5281 cggatgaaca ggcagacatc tgtgaatcgc ttcacgacca cgctgatgag ctttaccgca
     5341 ggtgggccat tctcatgaag aatatcttga atttattgtc atattactag ttggtgtgga
     5401 agtccatata tcggtgatca atatagtggt tgacatgctg gctagtcaac attgagcctt
     5461 ttgatcatgc aaatatatta cggtatttta caatcaaata tcaaacttaa ctattgactt
     5521 tataacttat ttaggtggta acattcttat aaaaaagaaa aaaattactg caaaacagta
     5581 ctagctttta acttgtatcc taggttatct atgctgtctc accatagaga atattaccta
     5641 tttcagaatg tatgtccatg attcgccggg taaatacata taatacacaa atctggctta
     5701 ataaagtcta taatatatct cataaagaag tgctaaattg gctagtgcta tatattttta
     5761 agaaaatttc ttttgactaa gtccatatcg actttgtaaa agttcacttt agcatacata
     5821 tattacacga gccagaaatt gtaacttttg cctaaaatca caaattgcaa aatttaattg
     5881 cttgcaaaag gtcacatgct tataatcaac ttttttaaaa atttaaaata cttttttatt
     5941 ttttattttt aaacataaat gaaataattt atttattgtt tatgattacc gaaacataaa
     6001 acctgctcaa gaaaaagaaa ctgttttgtc cttggaaaaa aagcactacc taggagcggc
     6061 caaaatgccg aggctttcat agcttaaact ctttacagaa aataggcatt atagatcagt
     6121 tcgagttttc ttattcttcc ttccggtttt atcgtcacag ttttacagta aataagtatc
     6181 acctcttaga gttaactatg agataagcaa gtatcatctc atttcattta cctgaagtcg
     6241 agtaaacaga aaatccaatt gttgatgaac ctcaatgact tagaactatc tatcggcaga
     6301 tcatataaag aggatttagg tacctagagg actgtacctg gagtatatat atatatatat
     6361 atatattatc tcaactatag tccatagagg tttctttctt gaggccttaa actgctaaag
     6421 aatgatattg gtggaatgca agcaccaatc tctcttcttt cgtaactgtt catatacttc
     6481 aaaccaagaa tgtaacgggc attgacccat ccaaaacctt cagtagctgc ccctttaaag
     6541 tcagcacctt gattaccgta ttctgcttca acacgatgag gatctgttcc tcttgtgaca
     6601 tcatattttt caaccacaat accattataa tcgacaaaag cctttgtcat catgaaaagc
     6661 catctataag ctagcctatt cgttacagtt aaataaccat aagaacggag gccttcccaa
     6721 gcaagaattt gatggggtgc ccaaccaaat ggatagtccc attgtctaat tggtctcgaa
     6781 atagaaattg ggcctcgaga acgctccgta catgcagcta aacctccaag catctctaac
     6841 ttgggtagtg ctttctccac cattttctgt gcttgctcct tcgtggcaag tccagcccat
     6901 aatgcccaga atgtagttgc ggattcgtat gacgttctgt gcttgatttt tgtgttgtag
     6961 tcaaagaaaa accccgactc gtcatcccac atatatttgg taattgatga ggcaacgcta
     7021 attatcaaca tatagattgt tatctatctg catgaacacg aaatctttac ttgacgactt
     7081 gaggctgatg gtgtttatgc aaagaaacca ctgtgtttaa tatgtgtcac tgtttgatat
     7141 tactgtcagc gtagaagata atagtaaaag cggttaataa gtgtatttga gataagtgtg
     7201 ataaagtttt tacagcgaaa agacgataaa tacaagaaaa tgattacgag gatacggaga
     7261 gaggtatgta catgtgtatt tatatactaa gctgccggcg gttgtttgca agaccgagaa
     7321 aaggctagca agaatcgggt cattgtagcg tatgcgcctg tgaacattct cttcaacaag
     7381 tttgattcca ttgcggtgaa atggtaaaag tcaaccccct gcgatgtata ttttcctgta
     7441 caatcaatca aaaagccaaa tgatttagca ttatctttac atcttgttat tttacagatt
     7501 ttatgtttag atcttttatg cttgcttttc aaaaggcctg caggcaagtg cacaaacaat
     7561 acttaaataa atactactca gtaataacct atttcttagc atttttgacg aaatttgcta
     7621 ttttgttaga gtcttttaca ccatttgtct ccacacctcc gcttacatca acaccaataa
     7681 cgccatttaa tctaagcgca tcaccaacat tttctggcgt cagtccacca gctaacataa
     7741 aatgtaagct ctgcctcgcg cgtttcggtg atgacggtga aaacctctga cacatgcagc
     7801 tcccggagac ggtcacagct tgtctgtaag cggatgccgg gagcagacaa gcccgtcagg
     7861 gcgcgtcagc gggtgttggc gggtgtcggg gcgcagccat gacccagtca cgtagcgata
     7921 gcggagtgta tactggctta actatgcggc atcagagcag attgtactga gagtgcacca
     7981 tatgcggtgt gaaataccgc acagatgcgt aaggagaaaa taccgcatca ggcgctcttc
     8041 cgcttcctcg ctcactgact cgctgcgctc ggtcgttcgg ctgcggcgag cggtatcagc
     8101 tcactcaaag gcggtaatac ggttatccac agaatcaggg gataacgcag gaaagaacat
     8161 gtgagcaaaa ggccagcaaa aggccaggaa ccgtaaaaag gccgcgttgc tggcgttttt
     8221 ccataggctc cgcccccctg acgagcatca caaaaatcga cgctcaagtc agaggtggcg
     8281 aaacccgaca ggactataaa gataccaggc gtttccccct ggaagctccc tcgtgcgctc
     8341 tcctgttccg accctgccgc ttaccggata cctgtccgcc tttctccctt cgggaagcgt
     8401 ggcgctttct catagctcac gctgtaggta tctcagttcg gtgtaggtcg ttcgctccaa
     8461 gctgggctgt gtgcacgaac cccccgttca gcccgaccgc tgcgccttat ccggtaacta
     8521 tcgtcttgag tccaacccgg taagacacga cttatcgcca ctggcagcag ccactggtaa
     8581 caggattagc agagcgaggt atgtaggcgg tgctacagag ttcttgaagt ggtggcctaa
     8641 ctacggctac actagaagga cagtatttgg tatctgcgct ctgctgaagc cagttacctt
     8701 cggaaaaaga gttggtagct cttgatccgg caaacaaacc accgctggta gcggtggttt
     8761 ttttgtttgc aagcagcaga ttacgcgcag aaaaaaagga tctcaagaag atcctttgat
     8821 cttttctacg gggtctgacg ctcagtggaa cgaaaactca cgttaaggga ttttggtcat
     8881 gagattatca aaaaggatct tcacctagat ccttttaaat taaaaatgaa gttttaaatc
     8941 aatctaaagt atatatgagt aaacttggtc tgacagttac caatgcttaa tcagtgaggc
     9001 acctatctca gcgatctgtc tatttcgttc atccatagtt gcctgactcc ccgtcgtgta
     9061 gataactacg atacgggagg gcttaccatc tggccccagt gctgcaatga taccgcgaga
     9121 cccacgctca ccggctccag atttatcagc aataaaccag ccagccggaa gggccgagcg
     9181 cagaagtggt cctgcaactt tatccgcctc catccagtct attaattgtt gccgggaagc
     9241 tagagtaagt agttcgccag ttaatagttt gcgcaacgtt gttgccattg ctgcaggcat
     9301 cgtggtgtca cgctcgtcgt ttggtatggc ttcattcagc tccggttccc aacgatcaag
     9361 gcgagttaca tgatccccca tgttgtgcaa aaaagcggtt agctccttcg gtcctccgat
     9421 cgttgtcaga agtaagttgg ccgcagtgtt atcactcatg gttatggcag cactgcataa
     9481 ttctcttact gtcatgccat ccgtaagatg cttttctgtg actggtgagt actcaaccaa
     9541 gtcattctga gaatagtgta tgcggcgacc gagttgctct tgcccggcgt caacacggga
     9601 taataccgcg ccacatagca gaactttaaa agtgctcatc attggaaaac gttcttcggg
     9661 gcgaaaactc tcaaggatct taccgctgtt gagatccagt tcgatgtaac ccactcgtgc
     9721 acccaactga tcttcagcat cttttacttt caccagcgtt tctgggtgag caaaaacagg
     9781 aaggcaaaat gccgcaaaaa agggaataag ggcgacacgg aaatgttgaa tactcatact
     9841 cttccttttt caatattatt gaagcattta tcagggttat tgtctcatga gcggatacat
     9901 atttgaatgt atttagaaaa ataaacaaat aggggttccg cgcacatttc cccgaaaagt
     9961 gccacctgac gtctaagaaa ccattattat catgacatta acctataaaa ataggcgtat
    10021 cacgaggccc tttcgtcttc aa
//
