LOCUS       40924_37678       10198 bp DNA     circular SYN 18-DEC-2018
DEFINITION  Cloning vector pRR22, complete sequence.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 10198)
  AUTHORS   Ramsden R, Arms L, Davis TN, Muller EG.
  TITLE     An intein with genetically selectable markers provides a new 
            approach to internally label proteins with GFP
  JOURNAL   BMC Biotechnol. 11 (1), 71 (2011)
  PUBMED    21708017
REFERENCE   2  (bases 1 to 10198)
  AUTHORS   Ramsden R, Arms L, Davis TN, Muller EGD.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-JUN-2011) Biochemistry, University of Washington, 1705
            NE Pacific St, HSB J461, Seattle, WA 98195, USA
REFERENCE   3  (bases 1 to 10198)
  TITLE     Direct Submission
REFERENCE   4  (bases 1 to 10198)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "BMC 
            Biotechnol."; date: "2011"; volume: "11"; issue: "1"; pages: "71"
COMMENT     SGRef: number: 2; type: "Journal Article"; journalName: "Submitted 
            (30-JUN-2011) Biochemistry, University of Washington, 1705 NE 
            Pacific St, HSB J461, Seattle, WA 98195, USA"
COMMENT     SGRef: number: 3; type: "Journal Article"
FEATURES             Location/Qualifiers
     source          1..10198
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        14..678
                     /label=GAL1,10 promoter
                     /note="divergent inducible promoter, regulated by Gal4"
     CDS             694..714
                     /codon_start=1
                     /label=SV40 NLS
                     /note="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /translation="PKKKRKV"
     CDS             724..744
                     /codon_start=1
                     /label=SV40 NLS
                     /note="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /translation="PKKKRKV"
     CDS             748..768
                     /codon_start=1
                     /label=SV40 NLS
                     /note="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /translation="PKKKRKV"
     CDS             781..1491
                     /codon_start=1
                     /label=GFP (S65T)
                     /note="S65T variant of Aequorea victoria green fluorescent 
                     protein (Heim et al., 1995)"
                     /translation="SKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTLKF
                     ICTTGKLPVPWPTLVTTFTYGVQCFSRYPDHMKRHDFFKSAMPEGYVQERTIFFKDDGN
                     YKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIKVN
                     FKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLLEF
                     VTAAGITHGMDELYK"
     CDS             1906..2709
                     /codon_start=1
                     /label=KanR
                     /note="aminoglycoside phosphotransferase"
                     /translation="GKEKTHVSRPRLNSNMDADLYGYKWARDNVGQSGATIYRLYGKPD
                     APELFLKHGKGSVANDVTDEMVRLNWLTEFMPLPTIKHFIRTPDDAWLLTTAIPGKTAF
                     QVLEEYPDSGENIVDALAVFLRRLHSIPVCNCPFNSDRVFRLAQAQSRMNNGLVDASDF
                     DDERNGWPVEQVWKEMHKLLPFSPDSVVTHGDFSLDNLIFDEGKLIGCIDVGRVGIADR
                     YQDLAILWNCLGEFSPSLQKRLFQKYGIDNPDMNKLQFHLMLDEFF"
     CDS             2875..2898
                     /codon_start=1
                     /label=FLAG
                     /note="FLAG(R) epitope tag, followed by an enterokinase
                     cleavage site"
                     /translation="DYKDDDDK"
     promoter        3688..3908
                     /label=URA3 promoter
     CDS             3909..4709
                     /codon_start=1
                     /label=URA3
                     /note="orotidine-5'-phosphate decarboxylase, required for
                     uracil biosynthesis"
                     /translation="MSKATYKERAATHPSPVAAKLFNIMHEKQTNLCASLDVRTTKELL
                     ELVEALGPKICLLKTHVDILTDFSMEGTVKPLKALSAKYNFLLFEDRKFADIGNTVKLQ
                     YSAGVYRIAEWADITNAHGVVGPGIVSGLKQAAEEVTKEPRGLLMLAELSCKGSLSTGE
                     YTKGTVDIAKSDKDFVIGFIAQRDMGGRDEGYDWLIMTPGVGLDDKGDALGQQYRTVDD
                     VVSTGSDIIIVGRGLFAKGRDAKVEGERYRKAGWEAYLRRCGQQN"
     misc_feature    complement(6057..7906)
                     /label=CEN/ARS
                     /note="S. cerevisiae CEN4 centromere fused to the
                     autonomously replicating sequence ARS1/ARS416"
     misc_feature    8048..8188
                     /label=bom
                     /note="basis of mobility region from pBR322"
     rep_origin      complement(8374..8962)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     CDS             complement(9136..9993)
                     /codon_start=1
                     /label=AmpR
                     /note="beta-lactamase"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRVDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPAAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     promoter        complement(9994..10098)
                     /label=AmpR promoter
ORIGIN
        1 gaattccttg aattttcaaa aattcttact ttttttttgg atggacgcaa agaagtttaa
       61 taatcatatt acatggcatt accaccatat acatatccat atacatatcc atatctaatc
      121 ttacttatat gttgtggaaa tgtaaagagc cccattatct tagcctaaaa aaaccttctc
      181 tttggaactt tcagtaatac gcttaactgc tcattgctat attgaagtac ggattagaag
      241 ccgccgagcg ggtgacagcc ctccgaagga agactctcct ccgtgcgtcc tcgtcttcac
      301 cggtcgcgtt cctgaaacgc agatgtgcct cgcgccgcac tgctccgaac aataaagatt
      361 ctacaatact agcttttatg gttatgaaga ggaaaaattg gcagtaacct ggccccacaa
      421 accttcaaat gaacgaatca aattaacaac cataggatga taatgcgatt agttttttag
      481 ccttatttct ggggtaatta atcagcgaag cgatgatttt tgatctatta acagatatat
      541 aaatgcaaaa actgcataac cactttaact aatactttca acattttcgg tttgtattac
      601 ttcttattca aatgtaataa aagtatcaac aaaaaattgt taatatacct ctatacttta
      661 acgtcaagga gaaaaaaccc cggatccatg gttccaaaga agaagagaaa ggttttaatt
      721 gatccaaaaa agaaaagaaa ggttgatcca aagaaaaaga gaaaagtttt aattaaagga
      781 agtaaaggag aagaactttt cactggagtt gtcccaattc ttgttgaatt agatggtgat
      841 gttaatgggc acaaattttc tgtcagtgga gagggtgaag gtgatgcaac atacggaaaa
      901 cttaccctta aatttatttg cactactgga aaactacctg ttccatggcc aacacttgtc
      961 actactttca cttatggtgt tcaatgcttt tcaagatacc cagatcatat gaaacggcat
     1021 gactttttca agagtgccat gcccgaaggt tatgtacagg aaagaactat atttttcaaa
     1081 gatgacggga actacaagac acgtgctgaa gtcaagtttg aaggtgatac ccttgttaat
     1141 agaatcgagt taaaaggtat tgattttaaa gaagatggaa acattcttgg acacaaattg
     1201 gaatacaact ataactcaca caatgtatac atcatggcag acaaacaaaa gaatggaatc
     1261 aaagttaact tcaaaattag acacaacatt gaagatggaa gcgttcaact agcagaccat
     1321 tatcaacaaa atactccaat tggcgatggc cctgtccttt taccagacaa ccattacctg
     1381 tccacacaat ctgccctttc gaaagatccc aacgaaaaga gagaccacat ggtccttctt
     1441 gagtttgtaa cagctgctgg gattacacat ggcatggatg aactatacaa aggtgctggt
     1501 gcaggtgcag gagctggagc aggtttctgg gagaaagcgt gtttggccaa gggaacccgt
     1561 ttgttgagat gcgatggaac cgagatcaat gtggaagacg tgcgcgaagg tgacctactt
     1621 ctgggtcccg atggagagcc tcgccgtgca ttcaacatag tgaatggcat cgacagactg
     1681 tacagaatca agatcggcgg tgagaaagag gaccttgtgg tgacgccgaa ccatattctg
     1741 gtgctttata gagaggatgg ttccaagaat gtggagaagc aaacggtgga gatcactgct
     1801 gccgagtttg ccgcgctttc taccgaggaa agaagcctct atagtgcctt tacatctcct
     1861 agggctgaga agggcgccgc aggtgcaggt gcaggtgcag ctagcggtaa ggaaaagact
     1921 cacgtttcga ggccgcgatt aaattccaac atggatgctg atttatatgg gtataaatgg
     1981 gctcgcgata atgtcgggca atcaggtgcg acaatctatc gattgtatgg gaagcccgat
     2041 gcgccagagt tgtttctgaa acatggcaaa ggtagcgttg ccaatgatgt tacagatgag
     2101 atggtcagac taaactggct gacggaattt atgcctcttc cgaccatcaa gcattttatc
     2161 cgtactcctg atgatgcatg gttactcacc actgcgatcc ccggcaaaac agcattccag
     2221 gtattagaag aatatcctga ttcaggtgaa aatattgttg atgcgctggc agtgttcctg
     2281 cgccggttgc attcgattcc tgtttgtaat tgtcctttta acagcgatcg cgtatttcgt
     2341 ctcgctcagg cgcaatcacg aatgaataac ggtttggttg atgcgagtga ttttgatgac
     2401 gagcgtaatg gctggcctgt tgaacaagtc tggaaagaaa tgcataagct tttgccattc
     2461 tcaccggatt cagtcgtcac tcatggtgat ttctcacttg ataaccttat ttttgacgag
     2521 gggaaattaa taggttgtat tgatgttgga cgagtcggaa tcgcagaccg ataccaggat
     2581 cttgccatcc tatggaactg cctcggtgag ttttctcctt cattacagaa acggcttttt
     2641 caaaaatatg gtattgataa tcctgatatg aataaattgc agtttcattt gatgctcgat
     2701 gagtttttcg gtaccgcggg tgctggtgct ggtgcttcgg ctcaaacgca cagtttcaag
     2761 attgagcaag ttagcctcga atccgagaag acagagtggg ctggtttccg agtcgacaaa
     2821 gatcagcttt acctgcgtca tgactacctt gtcctgcaca acagtggttt cgaagattac
     2881 aaggatgacg acgataagta agtcgaccga tgcccttgag agccttcaac ccagtcagct
     2941 ccttccggtg ggcgcggggc atgactatcg tcgccgcact tatgactgtc ttctttatca
     3001 tgcaactcgt aggacaggtg ccggcagcgc tctgggtcat tttcggcgag gaccgctttc
     3061 gctggagcgc gacgatgatc ggcctgtcgc ttgcggtatt cggaatcttg cacgccctcg
     3121 ctcaagcctt cgtcactggt cccgccacca aacgtttcgg cgagaagcag gccattatcg
     3181 ccggcatggc ggccgacgcg ctgggctacg tcttgctggc gttcgcgacg cgaggctgga
     3241 tggccttccc cattatgatt cttctcgctt ccggcggcat cgggatgccc gcgttgcagg
     3301 ccatgctgtc caggcaggta gatgacgacc atcagggaca gcttcaagga tcgctcgcgg
     3361 ctcttaccag cctaacttcg atcactggac cgctgatcgt cacggcgatt tatgccgcct
     3421 cggcgagcac atggaacggg ttggcatgga ttgtaggcgc cgccctatac cttgtctgcc
     3481 tccccgcgtt gcgtcgcggt gcatggagcc gggccacctc gacctgaatg gaagccggcg
     3541 gcacctcgct aacggattca ccactccaag aattggagcc aatcaattct tgcggagaac
     3601 tgtgaatgcg caaaccaacc cttggcagaa catatccatc gcgtccgcca tctccagcag
     3661 ccgcacgcgg cgcatcgggg ggggggtttc aattcaattc atcatttttt ttttattctt
     3721 ttttttgatt tcggtttctt tgaaattttt ttgattcggt aatctccgaa cagaaggaag
     3781 aacgaaggaa ggagcacaga cttagattgg tatatatacg catatgtagt gttgaagaaa
     3841 catgaaattg cccagtattc ttaacccaac tgcacagaac aaaaacctgc aggaaacgaa
     3901 gataaatcat gtcgaaagct acatataagg aacgtgctgc tactcatcct agtcctgttg
     3961 ctgccaagct atttaatatc atgcacgaaa agcaaacaaa cttgtgtgct tcattggatg
     4021 ttcgtaccac caaggaatta ctggagttag ttgaagcatt aggtcccaaa atttgtttac
     4081 taaaaacaca tgtggatatc ttgactgatt tttccatgga gggcacagtt aagccgctaa
     4141 aggcattatc cgccaagtac aattttttac tcttcgaaga cagaaaattt gctgacattg
     4201 gtaatacagt caaattgcag tactctgcgg gtgtatacag aatagcagaa tgggcagaca
     4261 ttacgaatgc acacggtgtg gtgggcccag gtattgttag cggtttgaag caggcggcag
     4321 aagaagtaac aaaggaacct agaggccttt tgatgttagc agaattgtca tgcaagggct
     4381 ccctatctac tggagaatat actaagggta ctgttgacat tgcgaagagc gacaaagatt
     4441 ttgttatcgg ctttattgct caaagagaca tgggtggaag agatgaaggt tacgattggt
     4501 tgattatgac acccggtgtg ggtttagatg acaagggaga cgcattgggt caacagtata
     4561 gaaccgtgga tgatgtggtc tctacaggat ctgacattat tattgttgga agaggactat
     4621 ttgcaaaggg aagggatgct aaggtagagg gtgaacgtta cagaaaagca ggctgggaag
     4681 catatttgag aagatgcggc cagcaaaact aaaaaactgt attataagta aatgcatgta
     4741 tactaaactc acaaattaga gcttcaattt aattatatca gttattaccc gggaatctcg
     4801 gtcgtaatga tttttataat gacgaaaaaa aaaaaattgg aaagaaaacc ccccccccgc
     4861 agcgttgggt cctggccacg ggtgcgcatg atcgtgctcc tgtcgttgag gacccggcta
     4921 ggctggcggg gttgccttac tggttagcag aatgaatcac cgatacgcga gcgaacgtga
     4981 agcgactgct gctgcaaaac gtctgcgacc tgagcaacaa catgaatggt cttcggtttc
     5041 cgtgtttcgt aaagtctgga aacgcggaag tcagcgccct gcaccattat gttccggatc
     5101 tgcatcgcag gatgctgctg gctaccctgt ggaacaccta catctgtatt aacgaagcgc
     5161 tggcattgac cctgagtgat ttttctctgg tcccgccgca tccataccgc cagttgttta
     5221 ccctcacaac gttccagtaa ccgggcatgt tcatcatcag taacccgtat cgtgagcatc
     5281 ctctctcgtt tcatcggtat cattaccccc atgaacagaa attccccctt acacggaggc
     5341 atcaagtgac caaacaggaa aaaaccgccc ttaacatggc ccgctttatc agaagccaga
     5401 cattaacgct tctggagaaa ctcaacgagc tggacgcgga tgaacaggca gacatctgtg
     5461 aatcgcttca cgaccacgct gatgagcttt accgcaggtg ggccattctc atgaagaata
     5521 tcttgaattt attgtcatat tactagttgg tgtggaagtc catatatcgg tgatcaatat
     5581 agtggttgac atgctggcta gtcaacattg agccttttga tcatgcaaat atattacggt
     5641 attttacaat caaatatcaa acttaactat tgactttata acttatttag gtggtaacat
     5701 tcttataaaa aagaaaaaaa ttactgcaaa acagtactag cttttaactt gtatcctagg
     5761 ttatctatgc tgtctcacca tagagaatat tacctatttc agaatgtatg tccatgattc
     5821 gccgggtaaa tacatataat acacaaatct ggcttaataa agtctataat atatctcata
     5881 aagaagtgct aaattggcta gtgctatata tttttaagaa aatttctttt gactaagtcc
     5941 atatcgactt tgtaaaagtt cactttagca tacatatatt acacgagcca gaaattgtaa
     6001 cttttgccta aaatcacaaa ttgcaaaatt taattgcttg caaaaggtca catgcttata
     6061 atcaactttt ttaaaaattt aaaatacttt tttatttttt atttttaaac ataaatgaaa
     6121 taatttattt attgtttatg attaccgaaa cataaaacct gctcaagaaa aagaaactgt
     6181 tttgtccttg gaaaaaaagc actacctagg agcggccaaa atgccgaggc tttcatagct
     6241 taaactcttt acagaaaata ggcattatag atcagttcga gttttcttat tcttccttcc
     6301 ggttttatcg tcacagtttt acagtaaata agtatcacct cttagagtta actatgagat
     6361 aagcaagtat catctcattt catttacctg aagtcgagta aacagaaaat ccaattgttg
     6421 atgaacctca atgacttaga actatctatc ggcagatcat ataaagagga tttaggtacc
     6481 tagaggactg tacctggagt atatatatat atatatatat attatctcaa ctatagtcca
     6541 tagaggtttc tttcttgagg ccttaaactg ctaaagaatg atattggtgg aatgcaagca
     6601 ccaatctctc ttctttcgta actgttcata tacttcaaac caagaatgta acgggcattg
     6661 acccatccaa aaccttcagt agctgcccct ttaaagtcag caccttgatt accgtattct
     6721 gcttcaacac gatgaggatc tgttcctctt gtgacatcat atttttcaac cacaatacca
     6781 ttataatcga caaaagcctt tgtcatcatg aaaagccatc tataagctag cctattcgtt
     6841 acagttaaat aaccataaga acggaggcct tcccaagcaa gaatttgatg gggtgcccaa
     6901 ccaaatggat agtcccattg tctaattggt ctcgaaatag aaattgggcc tcgagaacgc
     6961 tccgtacatg cagctaaacc tccaagcatc tctaacttgg gtagtgcttt ctccaccatt
     7021 ttctgtgctt gctccttcgt ggcaagtcca gcccataatg cccagaatgt agttgcggat
     7081 tcgtatgacg ttctgtgctt gatttttgtg ttgtagtcaa agaaaaaccc cgactcgtca
     7141 tcccacatat atttggtaat tgatgaggca acgctaatta tcaacatata gattgttatc
     7201 tatctgcatg aacacgaaat ctttacttga cgacttgagg ctgatggtgt ttatgcaaag
     7261 aaaccactgt gtttaatatg tgtcactgtt tgatattact gtcagcgtag aagataatag
     7321 taaaagcggt taataagtgt atttgagata agtgtgataa agtttttaca gcgaaaagac
     7381 gataaataca agaaaatgat tacgaggata cggagagagg tatgtacatg tgtatttata
     7441 tactaagctg ccggcggttg tttgcaagac cgagaaaagg ctagcaagaa tcgggtcatt
     7501 gtagcgtatg cgcctgtgaa cattctcttc aacaagtttg attccattgc ggtgaaatgg
     7561 taaaagtcaa ccccctgcga tgtatatttt cctgtacaat caatcaaaaa gccaaatgat
     7621 ttagcattat ctttacatct tgttatttta cagattttat gtttagatct tttatgcttg
     7681 cttttcaaaa ggcctgcagg caagtgcaca aacaatactt aaataaatac tactcagtaa
     7741 taacctattt cttagcattt ttgacgaaat ttgctatttt gttagagtct tttacaccat
     7801 ttgtctccac acctccgctt acatcaacac caataacgcc atttaatcta agcgcatcac
     7861 caacattttc tggcgtcagt ccaccagcta acataaaatg taagctctgc ctcgcgcgtt
     7921 tcggtgatga cggtgaaaac ctctgacaca tgcagctccc ggagacggtc acagcttgtc
     7981 tgtaagcgga tgccgggagc agacaagccc gtcagggcgc gtcagcgggt gttggcgggt
     8041 gtcggggcgc agccatgacc cagtcacgta gcgatagcgg agtgtatact ggcttaacta
     8101 tgcggcatca gagcagattg tactgagagt gcaccatatg cggtgtgaaa taccgcacag
     8161 atgcgtaagg agaaaatacc gcatcaggcg ctcttccgct tcctcgctca ctgactcgct
     8221 gcgctcggtc gttcggctgc ggcgagcggt atcagctcac tcaaaggcgg taatacggtt
     8281 atccacagaa tcaggggata acgcaggaaa gaacatgtga gcaaaaggcc agcaaaaggc
     8341 caggaaccgt aaaaaggccg cgttgctggc gtttttccat aggctccgcc cccctgacga
     8401 gcatcacaaa aatcgacgct caagtcagag gtggcgaaac ccgacaggac tataaagata
     8461 ccaggcgttt ccccctggaa gctccctcgt gcgctctcct gttccgaccc tgccgcttac
     8521 cggatacctg tccgcctttc tcccttcggg aagcgtggcg ctttctcata gctcacgctg
     8581 taggtatctc agttcggtgt aggtcgttcg ctccaagctg ggctgtgtgc acgaaccccc
     8641 cgttcagccc gaccgctgcg ccttatccgg taactatcgt cttgagtcca acccggtaag
     8701 acacgactta tcgccactgg cagcagccac tggtaacagg attagcagag cgaggtatgt
     8761 aggcggtgct acagagttct tgaagtggtg gcctaactac ggctacacta gaaggacagt
     8821 atttggtatc tgcgctctgc tgaagccagt taccttcgga aaaagagttg gtagctcttg
     8881 atccggcaaa caaaccaccg ctggtagcgg tggttttttt gtttgcaagc agcagattac
     8941 gcgcagaaaa aaaggatctc aagaagatcc tttgatcttt tctacggggt ctgacgctca
     9001 gtggaacgaa aactcacgtt aagggatttt ggtcatgaga ttatcaaaaa ggatcttcac
     9061 ctagatcctt ttaaattaaa aatgaagttt taaatcaatc taaagtatat atgagtaaac
     9121 ttggtctgac agttaccaat gcttaatcag tgaggcacct atctcagcga tctgtctatt
     9181 tcgttcatcc atagttgcct gactccccgt cgtgtagata actacgatac gggagggctt
     9241 accatctggc cccagtgctg caatgatacc gcgagaccca cgctcaccgg ctccagattt
     9301 atcagcaata aaccagccag ccggaagggc cgagcgcaga agtggtcctg caactttatc
     9361 cgcctccatc cagtctatta attgttgccg ggaagctaga gtaagtagtt cgccagttaa
     9421 tagtttgcgc aacgttgttg ccattgctgc aggcatcgtg gtgtcacgct cgtcgtttgg
     9481 tatggcttca ttcagctccg gttcccaacg atcaaggcga gttacatgat cccccatgtt
     9541 gtgcaaaaaa gcggttagct ccttcggtcc tccgatcgtt gtcagaagta agttggccgc
     9601 agtgttatca ctcatggtta tggcagcact gcataattct cttactgtca tgccatccgt
     9661 aagatgcttt tctgtgactg gtgagtactc aaccaagtca ttctgagaat agtgtatgcg
     9721 gcgaccgagt tgctcttgcc cggcgtcaac acgggataat accgcgccac atagcagaac
     9781 tttaaaagtg ctcatcattg gaaaacgttc ttcggggcga aaactctcaa ggatcttacc
     9841 gctgttgaga tccagttcga tgtaacccac tcgtgcaccc aactgatctt cagcatcttt
     9901 tactttcacc agcgtttctg ggtgagcaaa aacaggaagg caaaatgccg caaaaaaggg
     9961 aataagggcg acacggaaat gttgaatact catactcttc ctttttcaat attattgaag
    10021 catttatcag ggttattgtc tcatgagcgg atacatattt gaatgtattt agaaaaataa
    10081 acaaataggg gttccgcgca catttccccg aaaagtgcca cctgacgtct aagaaaccat
    10141 tattatcatg acattaacct ataaaaatag gcgtatcacg aggccctttc gtcttcaa
//
