LOCUS       40924_37673       12827 bp DNA     circular SYN 18-DEC-2018
DEFINITION  Promoter probe vector pRR21, complete sequence.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 12827)
  AUTHORS   Raveendra GM, Bhat RS, Kuruvinashetti MS.
  TITLE     Promoter probe vector with Sgfp65T reporter
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 12827)
  AUTHORS   Raveendra GM, Bhat RS, Kuruvinashetti MS.
  TITLE     Direct Submission
  JOURNAL   Submitted (28-MAY-2008) Department of Biotechnology, University of 
            Agricultural Sciences, Krishinagar, Dharwad, Karnataka 580 005, 
            India
REFERENCE   3  (bases 1 to 12827)
  TITLE     Direct Submission
REFERENCE   4  (bases 1 to 12827)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: 
            "Unpublished"
COMMENT     SGRef: number: 2; type: "Journal Article"; journalName: "Submitted 
            (28-MAY-2008) Department of Biotechnology, University of 
            Agricultural Sciences, Krishinagar, Dharwad, Karnataka 580 005, 
            India"
COMMENT     SGRef: number: 3; type: "Journal Article"
FEATURES             Location/Qualifiers
     source          1..12827
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     terminator      complement(7..259)
                     /label=NOS terminator
                     /note="nopaline synthase terminator and poly(A) signal"
     CDS             complement(285..1001)
                     /codon_start=1
                     /label=EGFP
                     /note="enhanced GFP"
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTFTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
                     EFVTAAGITHGMDELYK"
     primer_bind     complement(1041..1057)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     promoter        1434..1779
                     /label=CaMV 35S promoter
                     /note="strong constitutive promoter from cauliflower mosaic
                     virus"
     intron          1811..2000
                     /label=cat1 intron
                     /note="castor bean catalase intron, modified"
     CDS             1995..3818
                     /codon_start=1
                     /label=GUSPlus(TM)
                     /note="beta-glucuronidase"
                     /translation="LQNRRTSLYPINTETRGVFDLNGVWNFKLDYGKGLEEKWYESKLT
                     DTISMAVPSSYNDIGVTKEIRNHIGYVWYEREFTVPAYLKDQRIVLRFGSATHKAIVYV
                     NGELVVEHKGGFLPFEAEINNSLRDGMNRVTVAVDNILDDSTLPVGLYSERHEEGLGKV
                     IRNKPNFDFFNYAGLHRPVKIYTTPFTYVEDISVVTDFNGPTGTVTYTVDFQGKAETVK
                     VSVVDEEGKVVASTEGLSGNVEIPNVILWEPLNTYLYQIKVELVNDGLTIDVYEEPFGV
                     RTVEVNDGKFLINNKPFYFKGFGKHEDTPINGRGFNEASNVMDFNILKWIGANSFRTAH
                     YPYSEELMRLADREGLVVIDETPAVGVHLNFMATTGLGEGSERVSTWEKIRTFEHHQDV
                     LRELVSRDKNHPSVVMWSIANEAATEEEGAYEYFKPLVELTKELDPQKRPVTIVLFVMA
                     TPETDKVAELIDVIALNRYNGWYFDGGDLEAAKVHLRQEFHAWNKRCPGKPIMITEYGA
                     DTVAGFHDIDPVMFTEEYQVEYYQANHVVFDEFENFVGEQAWNFADFATSQGVMRVQGN
                     KKGVFTRDRKPKLAAHVFRERWTNIPDFGYKN"
     CDS             3825..3842
                     /codon_start=1
                     /label=6xHis
                     /note="6xHis affinity tag"
                     /translation="HHHHHH"
     terminator      3874..4126
                     /label=NOS terminator
                     /note="nopaline synthase terminator and poly(A) signal"
     misc_feature    4148..4172
                     /label=RB T-DNA repeat
                     /note="right border repeat from nopaline C58 T-DNA"
     CDS             5472..6098
                     /codon_start=1
                     /label=pVS1 StaA
                     /note="stability protein from the Pseudomonas plasmid pVS1
                     (Heeb et al., 2000)"
                     /translation="MKVIAVLNQKGGSGKTTIATHLARALQLAGADVLLVDSDPQGSAR
                     DWAAVREDQPLTVVGIDRPTIDRDVKAIGRRDFVVIDGAPQAADLAVSAIKAADFVLIP
                     VQPSPYDIWATADLVELVKQRIEVTDGRLQAAFVVSRAIKGTRIGGEVAEALAGYELPI
                     LESRITQRVSYPGTAAAGTTVLESEPEGDAAREVQALAAEIKSKLI"
     CDS             6535..7599
                     /codon_start=1
                     /label=pVS1 RepA
                     /note="replication protein from the Pseudomonas plasmid
                     pVS1 (Heeb et al., 2000)"
                     /translation="GRKPSGPVQIGAALGDDLVEKLKAAQAAQRQRIEAEARPGESWQA
                     AADRIRKESRQPPAAGAPSIRKPPKGDEQPDFFVPMLYDVGTRDSRSIMDVAVFRLSKR
                     DRRAGEVIRYELPDGHVEVSAGPAGMASVWDYDLVLMAVSHLTESMNRYREGKGDKPGR
                     VFRPHVADVLKFCRRADGGKQKDDLVETCIRLNTTHVAMQRTKKAKNGRLVTVSEGEAL
                     ISRYKIVKSETGRPEYIEIELADWMYREITEGKNPDVLTVHPDYFLIDPGIGRFLYRLA
                     RRAAGKAEARWLFKTIYERSGSAGEFKKFCFTVRKLIGSNDLPEYDLKEEAGQAGPILV
                     MRYRNLIEGEASAGS"
     rep_origin      7668..7862
                     /label=pVS1 oriV
                     /note="origin of replication for the Pseudomonas plasmid
                     pVS1 (Heeb et al., 2000)"
     misc_feature    8206..8346
                     /label=bom
                     /note="basis of mobility region from pBR322"
     rep_origin      complement(8532..9120)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     CDS             complement(9210..10001)
                     /codon_start=1
                     /label=KanR
                     /note="aminoglycoside phosphotransferase"
                     /translation="MAKMRISPELKKLIEKYRCVKDTEGMSPAKVYKLVGENENLYLKM
                     TDSRYKGTTYDVEREKDMMLWLEGKLPVPKVLHFERHDGWSNLLMSEADGVLCSEEYED
                     EQSPEKIIELYAECIRLFHSIDISDCPYTNSLDSRLAELDYLLNNDLADVDCENWEEDT
                     PFKDPRELYDFLKTEKPEEELVFSHGDLGDSNIFVKDGKVSGFIDLGRSGRADKWYDIA
                     FCVRSIREDIGEEQYVELFFDLLGIKPDWEKIKYYILLDELF"
     misc_feature    10426..10450
                     /label=LB T-DNA repeat
                     /note="left border repeat from nopaline C58 T-DNA"
     polyA_signal    complement(10528..10702)
                     /label=CaMV poly(A) signal
                     /note="cauliflower mosaic virus polyadenylation signal"
     CDS             complement(10745..11767)
                     /codon_start=1
                     /label=HygR
                     /note="aminoglycoside phosphotransferase from E. coli"
                     /translation="MKKPELTATSVEKFLIEKFDSVSDLMQLSEGEESRAFSFDVGGRG
                     YVLRVNSCADGFYKDRYVYRHFASAALPIPEVLDIGEFSESLTYCISRRAQGVTLQDLP
                     ETELPAVLQPVAEAMDAIAAADLSQTSGFGPFGPQGIGQYTTWRDFICAIADPHVYHWQ
                     TVMDDTVSASVAQALDELMLWAEDCPEVRHLVHADFGSNNVLTDNGRITAVIDWSEAMF
                     GDSQYEVANIFFWRPWLACMEQQTRYFERRHPELAGSPRLRAYMLRIGLDQLYQSLVDG
                     NFDDAAWAQGRCDAIVRSGAGTVGRTQIARRSAAVWTDGCVEVLADSGNRRPSTRPRAK
                     K"
     promoter        complement(11835..12512)
                     /label=CaMV 35S promoter (enhanced)
                     /note="cauliflower mosaic virus 35S promoter with a
                     duplicated enhancer region"
     protein_bind    12703..12724
                     /label=CAP binding site
                     /note="CAP binding activates transcription in the presence
                     of cAMP."
     promoter        12739..12769
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    12777..12793
                     /label=lac operator
                     /note="The lac repressor binds to the lac operator to
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     12801..12817
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
ORIGIN
        1 aattccgatc tagtaacata gatgacaccg cgcgcgataa tttatcctag tttgcgcgct
       61 atattttgtt ttctatcgcg tattaaatgt ataattgcgg gactctaatc ataaaaaccc
      121 atctcataaa taacgtcatg cattacatgt taattattac atgcttaacg taattcaaca
      181 gaaattatat gataatcatc gcaagaccgg caacaggatt caatcttaag aaactttatt
      241 gccaaatgtt tgaacgatcg gggaaattcg agctcggtac cttacttgta cagctcgtcc
      301 atgccgtgag tgatcccggc ggcggtcacg aactccagca ggaccatgtg atcgcgcttc
      361 tcgttggggt ctttgctcag ggcggactgg gtgctcaggt agtggttgtc gggcagcagc
      421 acggggccgt cgccgatggg ggtgttctgc tggtagtggt cggcgagctg cacgctcccg
      481 tcctcgatgt tgtggcggat cttgaagttc accttgatgc cgttcttctg cttgtcggcc
      541 atgatataga cgttgtggct gttgtagttg tactccagct tgtgccccag gatgttgccg
      601 tcctccttga agtcgatgcc cttcagctcg atgcggttca ccagggtgtc gccctcgaac
      661 ttcacctcgg cgcgggtctt gtagttgccg tcgtccttga agaagatggt gcgctcctgg
      721 acgtagcctt cgggcatggc ggacttgaag aagtcgtgct gcttcatgtg gtcggggtag
      781 cggctgaagc actgcacgcc gtaggtgaag gtggtcacga gggtgggcca gggcacgggc
      841 agcttgccgg tggtgcagat gaacttcagg gtcagcttgc cgtaggtggc atcgccctcg
      901 ccctcgccgg acacgctgaa cttgtggccg tttacgtcgc cgtccagctc gaccaggatg
      961 ggcaccaccc cggtgaacag ctcctcgccc ttgctcacca tggatcctct agagtcgacc
     1021 tgcaggcatg caagcttggc actggccgtc gttttacaac gtcgtgactg ggaaaaccct
     1081 ggcgttaccc aacttaatcg ccttgcagca catccccctt tcgccagctg gcgtaatagc
     1141 gaagaggccc gcaccgatcg cccttcccaa cagttgcgca gcctgaatgg cgaatgctag
     1201 agcagcttga gcttggatca gattgtcgtt tcccgccttc agtttagctt catggagtca
     1261 aagattcaaa tagaggacct aacagaactc gccgtaaaga ctggcgaaca gttcatacag
     1321 agtctcttac gactcaatga caagaagaaa atcttcgtca acatggtgga gcacgacaca
     1381 cttgtctact ccaaaaatat caaagataca gtctcagaag accaaagggc aattgagact
     1441 tttcaacaaa gggtaatatc cggaaacctc ctcggattcc attgcccagc tatctgtcac
     1501 tttattgtga agatagtgga aaaggaaggt ggctcctaca aatgccatca ttgcgataaa
     1561 ggaaaggcca tcgttgaaga tgcctctgcc gacagtggtc ccaaagatgg acccccaccc
     1621 acgaggagca tcgtggaaaa agaagacgtt ccaaccacgt cttcaaagca agtggattga
     1681 tgtgatatct ccactgacgt aagggatgac gcacaatccc actatccttc gcaagaccct
     1741 tcctctatat aaggaagttc atttcatttg gagagaacac gggggactct tgaccatggt
     1801 agatctgagg gtaaatttct agtttttctc cttcattttc ttggttagga cccttttctc
     1861 tttttatttt tttgagcttt gatctttctt taaactgatc tattttttaa ttgattggtt
     1921 atggtgtaaa tattacatag ctttaactga taatctgatt actttatttc gtgtgtctat
     1981 gatgatgatg atagttacag aaccgacgaa ctagtctgta cccgatcaac accgagaccc
     2041 gtggcgtctt cgacctcaat ggcgtctgga acttcaagct ggactacggg aaaggactgg
     2101 aagagaagtg gtacgaaagc aagctgaccg acactattag tatggccgtc ccaagcagtt
     2161 acaatgacat tggcgtgacc aaggaaatcc gcaaccatat cggatatgtc tggtacgaac
     2221 gtgagttcac ggtgccggcc tatctgaagg atcagcgtat cgtgctccgc ttcggctctg
     2281 caactcacaa agcaattgtc tatgtcaatg gtgagctggt cgtggagcac aagggcggat
     2341 tcctgccatt cgaagcggaa atcaacaact cgctgcgtga tggcatgaat cgcgtcaccg
     2401 tcgccgtgga caacatcctc gacgatagca ccctcccggt ggggctgtac agcgagcgcc
     2461 acgaagaggg cctcggaaaa gtcattcgta acaagccgaa cttcgacttc ttcaactatg
     2521 caggcctgca ccgtccggtg aaaatctaca cgaccccgtt tacgtacgtc gaggacatct
     2581 cggttgtgac cgacttcaat ggcccaaccg ggactgtgac ctatacggtg gactttcaag
     2641 gcaaagccga gaccgtgaaa gtgtcggtcg tggatgagga aggcaaagtg gtcgcaagca
     2701 ccgagggcct gagcggtaac gtggagattc cgaatgtcat cctctgggaa ccactgaaca
     2761 cgtatctcta ccagatcaaa gtggaactgg tgaacgacgg actgaccatc gatgtctatg
     2821 aagagccgtt cggcgtgcgg accgtggaag tcaacgacgg caagttcctc atcaacaaca
     2881 aaccgttcta cttcaagggc tttggcaaac atgaggacac tcctatcaac ggccgtggct
     2941 ttaacgaagc gagcaatgtg atggatttca atatcctcaa atggatcggc gccaacagct
     3001 tccggaccgc acactatccg tactctgaag agttgatgcg tcttgcggat cgcgagggtc
     3061 tggtcgtgat cgacgagact ccggcagttg gcgtgcacct caacttcatg gccaccacgg
     3121 gactcggcga aggcagcgag cgcgtcagta cctgggagaa gattcggacg tttgagcacc
     3181 atcaagacgt tctccgtgaa ctggtgtctc gtgacaagaa ccatccaagc gtcgtgatgt
     3241 ggagcatcgc caacgaggcg gcgactgagg aagagggcgc gtacgagtac ttcaagccgt
     3301 tggtggagct gaccaaggaa ctcgacccac agaagcgtcc ggtcacgatc gtgctgtttg
     3361 tgatggctac cccggagacg gacaaagtcg ccgaactgat tgacgtcatc gcgctcaatc
     3421 gctataacgg atggtacttc gatggcggtg atctcgaagc ggccaaagtc catctccgcc
     3481 aggaatttca cgcgtggaac aagcgttgcc caggaaagcc gatcatgatc actgagtacg
     3541 gcgcagacac cgttgcgggc tttcacgaca ttgatccagt gatgttcacc gaggaatatc
     3601 aagtcgagta ctaccaggcg aaccacgtcg tgttcgatga gtttgagaac ttcgtgggtg
     3661 agcaagcgtg gaacttcgcg gacttcgcga cctctcaggg cgtgatgcgc gtccaaggaa
     3721 acaagaaggg cgtgttcact cgtgaccgca agccgaagct cgccgcgcac gtctttcgcg
     3781 agcgctggac caacattcca gatttcggct acaagaacgc tagccatcac catcaccatc
     3841 acgtgtgaat tggtgaccag ctcgaatttc cccgatcgtt caaacatttg gcaataaagt
     3901 ttcttaagat tgaatcctgt tgccggtctt gcgatgatta tcatataatt tctgttgaat
     3961 tacgttaagc atgtaataat taacatgtaa tgcatgacgt tatttatgag atgggttttt
     4021 atgattagag tcccgcaatt atacatttaa tacgcgatag aaaacaaaat atagcgcgca
     4081 aactaggata aattatcgcg cgcggtgtca tctatgttac tagatcggga attaaactat
     4141 cagtgtttga caggatatat tggcgggtaa acctaagaga aaagagcgtt tattagaata
     4201 acggatattt aaaagggcgt gaaaaggttt atccgttcgt ccatttgtat gtgcatgcca
     4261 accacagggt tcccctcggg atcaaagtac tttgatccaa cccctccgct gctatagtgc
     4321 agtcggcttc tgacgttcag tgcagccgtc ttctgaaaac gacatgtcgc acaagtccta
     4381 agttacgcga caggctgccg ccctgccctt ttcctggcgt tttcttgtcg cgtgttttag
     4441 tcgcataaag tagaatactt gcgactagaa ccggagacat tacgccatga acaagagcgc
     4501 cgccgctggc ctgctgggct atgcccgcgt cagcaccgac gaccaggact tgaccaacca
     4561 acgggccgaa ctgcacgcgg ccggctgcac caagctgttt tccgagaaga tcaccggcac
     4621 caggcgcgac cgcccggagc tggccaggat gcttgaccac ctacgccctg gcgacgttgt
     4681 gacagtgacc aggctagacc gcctggcccg cagcacccgc gacctactgg acattgccga
     4741 gcgcatccag gaggccggcg cgggcctgcg tagcctggca gagccgtggg ccgacaccac
     4801 cacgccggcc ggccgcatgg tgttgaccgt gttcgccggc attgccgagt tcgagcgttc
     4861 cctaatcatc gaccgcaccc ggagcgggcg cgaggccgcc aaggcccgag gcgtgaagtt
     4921 tggcccccgc cctaccctca ccccggcaca gatcgcgcac gcccgcgagc tgatcgacca
     4981 ggaaggccgc accgtgaaag aggcggctgc actgcttggc gtgcatcgct cgaccctgta
     5041 ccgcgcactt gagcgcagcg aggaagtgac gcccaccgag gccaggcggc gcggtgcctt
     5101 ccgtgaggac gcattgaccg aggccgacgc cctggcggcc gccgagaatg aacgccaaga
     5161 ggaacaagca tgaaaccgca ccaggacggc caggacgaac cgtttttcat taccgaagag
     5221 atcgaggcgg agatgatcgc ggccgggtac gtgttcgagc cgcccgcgca cgtctcaacc
     5281 gtgcggctgc atgaaatcct ggccggtttg tctgatgcca agctggcggc ctggccggcc
     5341 agcttggccg ctgaagaaac cgagcgccgc cgtctaaaaa ggtgatgtgt atttgagtaa
     5401 aacagcttgc gtcatgcggt cgctgcgtat atgatgcgat gagtaaataa acaaatacgc
     5461 aaggggaacg catgaaggtt atcgctgtac ttaaccagaa aggcgggtca ggcaagacga
     5521 ccatcgcaac ccatctagcc cgcgccctgc aactcgccgg ggccgatgtt ctgttagtcg
     5581 attccgatcc ccagggcagt gcccgcgatt gggcggccgt gcgggaagat caaccgctaa
     5641 ccgttgtcgg catcgaccgc ccgacgattg accgcgacgt gaaggccatc ggccggcgcg
     5701 acttcgtagt gatcgacgga gcgccccagg cggcggactt ggctgtgtcc gcgatcaagg
     5761 cagccgactt cgtgctgatt ccggtgcagc caagccctta cgacatatgg gccaccgccg
     5821 acctggtgga gctggttaag cagcgcattg aggtcacgga tggaaggcta caagcggcct
     5881 ttgtcgtgtc gcgggcgatc aaaggcacgc gcatcggcgg tgaggttgcc gaggcgctgg
     5941 ccgggtacga gctgcccatt cttgagtccc gtatcacgca gcgcgtgagc tacccaggca
     6001 ctgccgccgc cggcacaacc gttcttgaat cagaacccga gggcgacgct gcccgcgagg
     6061 tccaggcgct ggccgctgaa attaaatcaa aactcatttg agttaatgag gtaaagagaa
     6121 aatgagcaaa agcacaaaca cgctaagtgc cggccgtccg agcgcacgca gcagcaaggc
     6181 tgcaacgttg gccagcctgg cagacacgcc agccatgaag cgggtcaact ttcagttgcc
     6241 ggcggaggat cacaccaagc tgaagatgta cgcggtacgc caaggcaaga ccattaccga
     6301 gctgctatct gaatacatcg cgcagctacc agagtaaatg agcaaatgaa taaatgagta
     6361 gatgaatttt agcggctaaa ggaggcggca tggaaaatca agaacaacca ggcaccgacg
     6421 ccgtggaatg ccccatgtgt ggaggaacgg gcggttggcc aggcgtaagc ggctgggttg
     6481 tctgccggcc ctgcaatggc actggaaccc ccaagcccga ggaatcggcg tgacggtcgc
     6541 aaaccatccg gcccggtaca aatcggcgcg gcgctgggtg atgacctggt ggagaagttg
     6601 aaggccgcgc aggccgccca gcggcaacgc atcgaggcag aagcacgccc cggtgaatcg
     6661 tggcaagcgg ccgctgatcg aatccgcaaa gaatcccggc aaccgccggc agccggtgcg
     6721 ccgtcgatta ggaagccgcc caagggcgac gagcaaccag attttttcgt tccgatgctc
     6781 tatgacgtgg gcacccgcga tagtcgcagc atcatggacg tggccgtttt ccgtctgtcg
     6841 aagcgtgacc gacgagctgg cgaggtgatc cgctacgagc ttccagacgg gcacgtagag
     6901 gtttccgcag ggccggccgg catggccagt gtgtgggatt acgacctggt actgatggcg
     6961 gtttcccatc taaccgaatc catgaaccga taccgggaag ggaagggaga caagcccggc
     7021 cgcgtgttcc gtccacacgt tgcggacgta ctcaagttct gccggcgagc cgatggcgga
     7081 aagcagaaag acgacctggt agaaacctgc attcggttaa acaccacgca cgttgccatg
     7141 cagcgtacga agaaggccaa gaacggccgc ctggtgacgg tatccgaggg tgaagccttg
     7201 attagccgct acaagatcgt aaagagcgaa accgggcggc cggagtacat cgagatcgag
     7261 ctagctgatt ggatgtaccg cgagatcaca gaaggcaaga acccggacgt gctgacggtt
     7321 caccccgatt actttttgat cgatcccggc atcggccgtt ttctctaccg cctggcacgc
     7381 cgcgccgcag gcaaggcaga agccagatgg ttgttcaaga cgatctacga acgcagtggc
     7441 agcgccggag agttcaagaa gttctgtttc accgtgcgca agctgatcgg gtcaaatgac
     7501 ctgccggagt acgatttgaa ggaggaggcg gggcaggctg gcccgatcct agtcatgcgc
     7561 taccgcaacc tgatcgaggg cgaagcatcc gccggttcct aatgtacgga gcagatgcta
     7621 gggcaaattg ccctagcagg ggaaaaaggt cgaaaaggtc tctttcctgt ggatagcacg
     7681 tacattggga acccaaagcc gtacattggg aaccggaacc cgtacattgg gaacccaaag
     7741 ccgtacattg ggaaccggtc acacatgtaa gtgactgata taaaagagaa aaaaggcgat
     7801 ttttccgcct aaaactcttt aaaacttatt aaaactctta aaacccgcct ggcctgtgca
     7861 taactgtctg gccagcgcac agccgaagag ctgcaaaaag cgcctaccct tcggtcgctg
     7921 cgctccctac gccccgccgc ttcgcgtcgg cctatcgcgg ccgctggccg ctcaaaaatg
     7981 gctggcctac ggccaggcaa tctaccaggg cgcggacaag ccgcgccgtc gccactcgac
     8041 cgccggcgcc cacatcaagg caccctgcct cgcgcgtttc ggtgatgacg gtgaaaacct
     8101 ctgacacatg cagctcccgg agacggtcac agcttgtctg taagcggatg ccgggagcag
     8161 acaagcccgt cagggcgcgt cagcgggtgt tggcgggtgt cggggcgcag ccatgaccca
     8221 gtcacgtagc gatagcggag tgtatactgg cttaactatg cggcatcaga gcagattgta
     8281 ctgagagtgc accatatgcg gtgtgaaata ccgcacagat gcgtaaggag aaaataccgc
     8341 atcaggcgct cttccgcttc ctcgctcact gactcgctgc gctcggtcgt tcggctgcgg
     8401 cgagcggtat cagctcactc aaaggcggta atacggttat ccacagaatc aggggataac
     8461 gcaggaaaga acatgtgagc aaaaggccag caaaaggcca ggaaccgtaa aaaggccgcg
     8521 ttgctggcgt ttttccatag gctccgcccc cctgacgagc atcacaaaaa tcgacgctca
     8581 agtcagaggt ggcgaaaccc gacaggacta taaagatacc aggcgtttcc ccctggaagc
     8641 tccctcgtgc gctctcctgt tccgaccctg ccgcttaccg gatacctgtc cgcctttctc
     8701 ccttcgggaa gcgtggcgct ttctcatagc tcacgctgta ggtatctcag ttcggtgtag
     8761 gtcgttcgct ccaagctggg ctgtgtgcac gaaccccccg ttcagcccga ccgctgcgcc
     8821 ttatccggta actatcgtct tgagtccaac ccggtaagac acgacttatc gccactggca
     8881 gcagccactg gtaacaggat tagcagagcg aggtatgtag gcggtgctac agagttcttg
     8941 aagtggtggc ctaactacgg ctacactaga aggacagtat ttggtatctg cgctctgctg
     9001 aagccagtta ccttcggaaa aagagttggt agctcttgat ccggcaaaca aaccaccgct
     9061 ggtagcggtg gtttttttgt ttgcaagcag cagattacgc gcagaaaaaa aggatctcaa
     9121 gaagatcctt tgatcttttc tacggggtct gacgctcagt ggaacgaaaa ctcacgttaa
     9181 gggattttgg tcatgcattc taggtactaa aacaattcat ccagtaaaat ataatatttt
     9241 attttctccc aatcaggctt gatccccagt aagtcaaaaa atagctcgac atactgttct
     9301 tccccgatat cctccctgat cgaccggacg cagaaggcaa tgtcatacca cttgtccgcc
     9361 ctgccgcttc tcccaagatc aataaagcca cttactttgc catctttcac aaagatgttg
     9421 ctgtctccca ggtcgccgtg ggaaaagaca agttcctctt cgggcttttc cgtctttaaa
     9481 aaatcataca gctcgcgcgg atctttaaat ggagtgtctt cttcccagtt ttcgcaatcc
     9541 acatcggcca gatcgttatt cagtaagtaa tccaattcgg ctaagcggct gtctaagcta
     9601 ttcgtatagg gacaatccga tatgtcgatg gagtgaaaga gcctgatgca ctccgcatac
     9661 agctcgataa tcttttcagg gctttgttca tcttcatact cttccgagca aaggacgcca
     9721 tcggcctcac tcatgagcag attgctccag ccatcatgcc gttcaaagtg caggaccttt
     9781 ggaacaggca gctttccttc cagccatagc atcatgtcct tttcccgttc cacatcatag
     9841 gtggtccctt tataccggct gtccgtcatt tttaaatata ggttttcatt ttctcccacc
     9901 agcttatata ccttagcagg agacattcct tccgtatctt ttacgcagcg gtatttttcg
     9961 atcagttttt tcaattccgg tgatattctc attttagcca tttattattt ccttcctctt
    10021 ttctacagta tttaaagata ccccaagaag ctaattataa caagacgaac tccaattcac
    10081 tgttccttgc attctaaaac cttaaatacc agaaaacagc tttttcaaag ttgttttcaa
    10141 agttggcgta taacatagta tcgacggagc cgattttgaa accgcggtga tcacaggcag
    10201 caacgctctg tcatcgttac aatcaacatg ctaccctccg cgagatcatc cgtgtttcaa
    10261 acccggcagc ttagttgccg ttcttccgaa tagcatcggt aacatgagca aagtctgccg
    10321 ccttacaacg gctctcccgc tgacgccgtc ccggactgat gggctgcctg tatcgagtgg
    10381 tgattttgtg ccgagctgcc ggtcggggag ctgttggctg gctggtggca ggatatattg
    10441 tggtgtaaac aaattgacgc ttagacaact taataacaca ttgcggacgt ttttaatgta
    10501 ctgaattaac gccgaattaa ttcgggggat ctggatttta gtactggatt ttggttttag
    10561 gaattagaaa ttttattgat agaagtattt tacaaataca aatacatact aagggtttct
    10621 tatatgctca acacatgagc gaaaccctat aggaacccta attcccttat ctgggaacta
    10681 ctcacacatt attatggaga aactcgagct tgtcgatcga cagatccggt cggcatctac
    10741 tctatttctt tgccctcgga cgagtgctgg ggcgtcggtt tccactatcg gcgagtactt
    10801 ctacacagcc atcggtccag acggccgcgc ttctgcgggc gatttgtgta cgcccgacag
    10861 tcccggctcc ggatcggacg attgcgtcgc atcgaccctg cgcccaagct gcatcatcga
    10921 aattgccgtc aaccaagctc tgatagagtt ggtcaagacc aatgcggagc atatacgccc
    10981 ggagtcgtgg cgatcctgca agctccggat gcctccgctc gaagtagcgc gtctgctgct
    11041 ccatacaagc caaccacggc ctccagaaga agatgttggc gacctcgtat tgggaatccc
    11101 cgaacatcgc ctcgctccag tcaatgaccg ctgttatgcg gccattgtcc gtcaggacat
    11161 tgttggagcc gaaatccgcg tgcacgaggt gccggacttc ggggcagtcc tcggcccaaa
    11221 gcatcagctc atcgagagcc tgcgcgacgg acgcactgac ggtgtcgtcc atcacagttt
    11281 gccagtgata cacatgggga tcagcaatcg cgcatatgaa atcacgccat gtagtgtatt
    11341 gaccgattcc ttgcggtccg aatgggccga acccgctcgt ctggctaaga tcggccgcag
    11401 cgatcgcatc catagcctcc gcgaccggtt gtagaacagc gggcagttcg gtttcaggca
    11461 ggtcttgcaa cgtgacaccc tgtgcacggc gggagatgca ataggtcagg ctctcgctaa
    11521 actccccaat gtcaagcact tccggaatcg ggagcgcggc cgatgcaaag tgccgataaa
    11581 cataacgatc tttgtagaaa ccatcggcgc agctatttac ccgcaggaca tatccacgcc
    11641 ctcctacatc gaagctgaaa gcacgagatt cttcgccctc cgagagctgc atcaggtcgg
    11701 agacgctgtc gaacttttcg atcagaaact tctcgacaga cgtcgcggtg agttcaggct
    11761 ttttcatatc tcattgcccc ccgggatctg cgaaagctcg agagagatag atttgtagag
    11821 agagactggt gatttcagcg tgtcctctcc aaatgaaatg aacttcctta tatagaggaa
    11881 ggtcttgcga aggatagtgg gattgtgcgt catcccttac gtcagtggag atatcacatc
    11941 aatccacttg ctttgaagac gtggttggaa cgtcttcttt ttccacgatg ctcctcgtgg
    12001 gtgggggtcc atctttggga ccactgtcgg cagaggcatc ttgaacgata gcctttcctt
    12061 tatcgcaatg atggcatttg taggtgccac cttccttttc tactgtcctt ttgatgaagt
    12121 gacagatagc tgggcaatgg aatccgagga ggtttcccga tattaccctt tgttgaaaag
    12181 tctcaatagc cctttggtct tctgagactg tatctttgat attcttggag tagacgagag
    12241 tgtcgtgctc caccatgtta tcacatcaat ccacttgctt tgaagacgtg gttggaacgt
    12301 cttctttttc cacgatgctc ctcgtgggtg ggggtccatc tttgggacca ctgtcggcag
    12361 aggcatcttg aacgatagcc tttcctttat cgcaatgatg gcatttgtag gtgccacctt
    12421 ccttttctac tgtccttttg atgaagtgac agatagctgg gcaatggaat ccgaggaggt
    12481 ttcccgatat taccctttgt tgaaaagtct caatagccct ttggtcttct gagactgtat
    12541 ctttgatatt cttggagtag acgagagtgt cgtgctccac catgttggca agctgctcta
    12601 gccaatacgc aaaccgcctc tccccgcgcg ttggccgatt cattaatgca gctggcacga
    12661 caggtttccc gactggaaag cgggcagtga gcgcaacgca attaatgtga gttagctcac
    12721 tcattaggca ccccaggctt tacactttat gcttccggct cgtatgttgt gtggaattgt
    12781 gagcggataa caatttcaca caggaaacag ctatgaccat gattacg
//
