LOCUS       40924_37563        7686 bp DNA     circular SYN 18-DEC-2018
DEFINITION  Cloning vector pRP-M, complete sequence.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 7686)
  AUTHORS   Soysa R, Tran KD, Ullman B, Yates PA.
  TITLE     Integrating ribosomal promoter vectors that offer a choice of 
            constitutive expression profiles in Leishmania donovani
  JOURNAL   Mol. Biochem. Parasitol. (2016) In press
  PUBMED    26844641
REFERENCE   2  (bases 1 to 7686)
  AUTHORS   Yates PA, Ullman B, Soysa R.
  TITLE     Direct Submission
  JOURNAL   
REFERENCE   3  (bases 1 to 7686)
  TITLE     Direct Submission
REFERENCE   4  (bases 1 to 7686)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Mol. 
            Biochem. Parasitol. (2016) In press"
COMMENT     SGRef: number: 2; type: "Journal Article"; journalName: "Submitted 
            (17-DEC-2015) Biochemistry "
COMMENT     SGRef: number: 3; type: "Journal Article"
COMMENT     ##Assembly-Data-START##
            Sequencing Technology :: Sanger dideoxy sequencing 
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..7686
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     CDS             981..1781
                     /label=NeoR/KanR
                     /note="aminoglycoside phosphotransferase from Tn5"
     polyA_signal    1791..1839
                     /label=HSV TK poly(A) signal
                     /note="herpes simplex virus thymidine kinase
                     polyadenylation signal (Cole and Stacy, 1985)"
     CDS             complement(1962..1973)
                     /label=Factor Xa site
                     /note="Factor Xa recognition and cleavage site"
     rep_origin      complement(3462..4050)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     CDS             complement(4224..5081)
                     /label=AmpR
                     /note="beta-lactamase"
     promoter        complement(5082..5186)
                     /label=AmpR promoter
     misc_feature    5356..6003
                     /note="derived from Leishmania donovani ribosomal RNA
                     spacer region"
     regulatory      6030..6579
                     /note="derived from Leishmania donovani ribosomal RNA
                     genes"
                     /regulatory_class="promoter"
     misc_feature    6601..6682
                     /note="contains artificial trans-splice signal and 5-UTR; 
                     leishmanial consensus splice acceptor site; LdPARP"
     misc_feature    6689..6776
                     /label=multiple cloning site
                     /note="multiple cloning site"
     misc_feature    6787..7671
                     /note="derived from Leishmania major alpha tubulin
                     intergenic region; LmTub"
ORIGIN
        1 cctgcaggga gagaatggtt gaccatgaga cgccgatggg cgatgcgcca gcgcgtgctt
       61 gcgtacttca atcgtttttc atcttgattc agagctaagt agtagcacac cacagcaagc
      121 gtaatcatgt agcatgtatg caggtgcacg agtaggcact ctctgtctct gtccggcatc
      181 tatgatgctc tccggccgcg tgcgcgcgac gcctctgtgt gagtgctcgt gtgagtgtgt
      241 gagagctgtc caggcctttc ctctgcctac tcgtcccacg ctcaggcagg cacagaggaa
      301 tgaaaaggaa gacgaggacg caacgccagg cgcatcgaca tcgcgcacgt gtttgcggtg
      361 tactgtgcgt cgatgatcga gcggccaagc gtccagcttc acgcgaggag gactgcatcg
      421 ctgctcctgt gcgcgtgcgt gcacgcgtca aggcacgctc gattcgttcc cagggtggat
      481 accctccccc ctcccccagc tgcagacaac ccccaccctc tcgaggcttg cacccaggct
      541 cgtcagtctc tctaccactt tctgccttct gtagtgcacc ccacccctgc attctcctct
      601 ctctcacaca cacacacaca cacacacaca cacacacaca cacacgtaca cacgtacaca
      661 cacacgtgcg catgcttgac gcatacgcga cgaattcgaa agctcacctc attcctccct
      721 cctcacacca tcatcggcat ccatagagac acgtgcgcgt agaacgatac agctcacgcg
      781 cacagagagg cgtgttctgg tcgtgcgcat catcggcaca gcactggcgg acgaaactcg
      841 cacacaggca cgccgcctcc tttcacccgt catagatagt tgaattagac gccctcctcc
      901 tccctcatca tcgccgtcgt catccgggtc cgagcactag tcgcgtgtgg cctcgaacac
      961 cgagcgaccc tgcagccaat atgggatcgg ccattgaaca agatggattg cacgcaggtt
     1021 ctccggccgc ttgggtggag aggctattcg gctatgactg ggcacaacag acaatcggct
     1081 gctctgatgc cgccgtgttc cggctgtcag cgcaggggcg cccggttctt tttgtcaaga
     1141 ccgacctgtc cggtgccctg aatgaactgc aggacgaggc agcgcggcta tcgtggctgg
     1201 ccacgacggg cgttccttgc gcagctgtgc tcgacgttgt cactgaagcg ggaagggact
     1261 ggctgctatt gggcgaagtg ccggggcagg atctcctgtc atctcacctt gctcctgccg
     1321 agaaagtatc catcatggct gatgcaatgc ggcggctgca tacgcttgat ccggctacct
     1381 gcccattcga ccaccaagcg aaacatcgca tcgagcgagc acgtactcgg atggaagccg
     1441 gtcttgtcga tcaggatgat ctggacgaag agcatcaggg gctcgcgcca gccgaactgt
     1501 tcgccaggct caaggcgcgc atgcccgacg gcgaggatct cgtcgtgacc catggcgatg
     1561 cctgcttgcc gaatatcatg gtggaaaatg gccgcttttc tggattcatc gactgtggcc
     1621 ggctgggtgt ggcggaccgc tatcaggaca tagcgttggc tacccgtgat attgctgaag
     1681 agcttggcgg cgaatgggct gaccgcttcc tcgtgcttta cggtatcgcc gctcccgatt
     1741 cgcagcgcat cgccttctat cgccttcttg acgagttctt ctgaggggat cggcaataaa
     1801 aagacagaat aaaacgcacg ggtgttgggt cgtttgttcg gatcactagt gatcccgccc
     1861 gcacccgcgc gcgctcaggc cgcgtgtcgt cgtgctcttt ccattttttt tttgtgtgtg
     1921 gtggtgattt gagctgctct ccgttgtgtg ctggggaccc tccttccctc gatctcctcg
     1981 tgcggggtct ccgacgcgca gacgcggtgc gtgcgagcgt gcacgtgctg tcgccgtggc
     2041 tgtagttgcg agcggagaga gagagagagg gagggaggga gggcagaggg cagaggacat
     2101 gcgggtggga acgtgcaccg gcctcgtctc acgcagctgg agcccacgaa tgccaccacc
     2161 acacccttct tccccccccc tccctcttct tcccacggcg gcgacgacga cgggggctca
     2221 gctcacgctt ctgtagggta ttatattaaa gcacatgtgc gtgctgtgct tcggcttctt
     2281 gttggtggtc gttcgctttc aagtccacga ctcctgccgt tgctcaccgc cgggctgtct
     2341 tctttcgccc ctccctcggt gcctcttccg ccgccgcacg cgtgcgctga tcacgcgtct
     2401 tgcgtgatgt gcctgtgtgt atacacaccg tgcacggaga gagcgagcga gcgagagaga
     2461 gagagggcga gaggaagaat ggccggtgcc tcgcgtgggc aagcgtgcgc gagtttgtcg
     2521 tgtcgctgcg ccgcgtggat gtcgcgcagc gcaagacccc gtgcgcctga aacgtgaagg
     2581 gaggtcgaga agcgtgccgc atgaggcctt aaggcaggaa agtgaaaaga gctcgacgcg
     2641 agtgcagcac gcgcgacatc gaacaggacg gaatagacac tcagcccctt cccctttcaa
     2701 aaactgaatg gacggacatc gtaacgcgct tctctcccct ccactccccc tgtccctgtc
     2761 tccttgacgt gtgctgctct cgtgagcgac agatgaagcg aggcgtagaa ggaaaggggg
     2821 gggagcgagg cctgcacaca cacacacaca cacacacaca cacacacaca cacacacaca
     2881 cacacacaca cgaaaagcaa gtttgaggca gacgtggttt cacacgattc ctgtgcaaga
     2941 agcgtgcctg cgtgacggtg tggctgtgta tgcatgcgtg aagggttacc gcaacgatgc
     3001 gcaatggact cccccgcttt ccatttcgtc accttccgcc tctctctctc tctctctcac
     3061 catctacgcg tgcacatcat caactgtctc ttgtcggtgc tcaccaccct caaccacccc
     3121 tcactttcaa ggcttcccga acgcacacaa aaggcgtgaa aaccgctcgc gtgtgttgag
     3181 ccgtccaccg gcgcgccggc cgcggatcga aacctgtcgt gccagctgca ttaatgaatc
     3241 ggccaacgcg cggggagagg cggtttgcgt attgggcgct cttccgcttc ctcgctcact
     3301 gactcgctgc gctcggtcgt tcggctgcgg cgagcggtat cagctcactc aaaggcggta
     3361 atacggttat ccacagaatc aggggataac gcaggaaaga acatgtgagc aaaaggccag
     3421 caaaaggcca ggaaccgtaa aaaggccgcg ttgctggcgt ttttccatag gctccgcccc
     3481 cctgacgagc atcacaaaaa tcgacgctca agtcagaggt ggcgaaaccc gacaggacta
     3541 taaagatacc aggcgtttcc ccctggaagc tccctcgtgc gctctcctgt tccgaccctg
     3601 ccgcttaccg gatacctgtc cgcctttctc ccttcgggaa gcgtggcgct ttctcatagc
     3661 tcacgctgta ggtatctcag ttcggtgtag gtcgttcgct ccaagctggg ctgtgtgcac
     3721 gaaccccccg ttcagcccga ccgctgcgcc ttatccggta actatcgtct tgagtccaac
     3781 ccggtaagac acgacttatc gccactggca gcagccactg gtaacaggat tagcagagcg
     3841 aggtatgtag gcggtgctac agagttcttg aagtggtggc ctaactacgg ctacactaga
     3901 aggacagtat ttggtatctg cgctctgctg aagccagtta ccttcggaaa aagagttggt
     3961 agctcttgat ccggcaaaca aaccaccgct ggtagcggtg gtttttttgt ttgcaagcag
     4021 cagattacgc gcagaaaaaa aggatctcaa gaagatcctt tgatcttttc tacggggtct
     4081 gacgctcagt ggaacgaaaa ctcacgttaa gggattttgg tcatgagatt atcaaaaagg
     4141 atcttcacct agatcctttt aaattaaaaa tgaagtttta aatcaatcta aagtatatat
     4201 gagtaaactt ggtctgacag ttaccaatgc ttaatcagtg aggcacctat ctcagcgatc
     4261 tgtctatttc gttcatccat agttgcctga ctccccgtcg tgtagataac tacgatacgg
     4321 gagggcttac catctggccc cagtgctgca atgataccgc gagacccacg ctcaccggct
     4381 ccagatttat cagcaataaa ccagccagcc ggaagggccg agcgcagaag tggtcctgca
     4441 actttatccg cctccatcca gtctattaat tgttgccggg aagctagagt aagtagttcg
     4501 ccagttaata gtttgcgcaa cgttgttgcc attgctacag gcatcgtggt gtcacgctcg
     4561 tcgtttggta tggcttcatt cagctccggt tcccaacgat caaggcgagt tacatgatcc
     4621 cccatgttgt gcaaaaaagc ggttagctcc ttcggtcctc cgatcgttgt cagaagtaag
     4681 ttggccgcag tgttatcact catggttatg gcagcactgc ataattctct tactgtcatg
     4741 ccatccgtaa gatgcttttc tgtgactggt gagtactcaa ccaagtcatt ctgagaatag
     4801 tgtatgcggc gaccgagttg ctcttgcccg gcgtcaatac gggataatac cgcgccacat
     4861 agcagaactt taaaagtgct catcattgga aaacgttctt cggggcgaaa actctcaagg
     4921 atcttaccgc tgttgagatc cagttcgatg taacccactc gtgcacccaa ctgatcttca
     4981 gcatctttta ctttcaccag cgtttctggg tgagcaaaaa caggaaggca aaatgccgca
     5041 aaaaagggaa taagggcgac acggaaatgt tgaatactca tactcttcct ttttcaatat
     5101 tattgaagca tttatcaggg ttattgtctc atgagcggat acatatttga atgtatttag
     5161 aaaaataaac aaataggggt tccgcgcaca tttccccgaa aagtgccacc taaattgtaa
     5221 gcgttaatat tttgttaaaa ttcgcgttaa atttttgtta aatcagctca ttttttaacc
     5281 aataggccga aatcggcaaa atcccttata aatcaaaaga atagaccgag atagggttga
     5341 gtgttgttcc acacaaaata taggaggggg gggggtgaat acgttatata tgtataatgt
     5401 gtgtcgctta gcttcacaaa cccagaagga acggtgcaaa ggacccgaca accagctgtg
     5461 tgcgcgtata tatttacgtc tgcatacacg gctcgttgtc gggtccttgc ccttttccgt
     5521 ccccttttcc gtcctgcgcg tgtgtgtatg ggcgcgggtg gggtttgtgc atgcgggggg
     5581 gggggaggat cgagcacccc ccaacactac ctccatccaa aacaaacaca cagcagagag
     5641 tgcccgccaa ccttgggcgc ttctcggttg ccgtgtgtgt cattgccctg cagagagggc
     5701 ggaacagggg ggtggaggtg ggaatagggg ggggggcacg atttccaccg ccaaagtctc
     5761 tccctacggg aacacgccaa taggcatgcc ctttgactgt aagttacagt caagcaatac
     5821 ggcggcttac ctccgccacc cgacccggca accagctgcg cccaaccgcg cggctcgttg
     5881 tcgggtcctt gccctttttt cactgcgacc ctcaaagaca gtgtacgcca gtccaacgat
     5941 cccactccca gcgatggacc gcgcccaacg ctcacccgca acccacattc ggaattcgac
     6001 atcatgccct aggatttaaa tgcggccgcg aggtctgcga ttgacgtagg agttgcaagg
     6061 gggagggggt atgaacgggg tgggtagagc ttttttaggt ggaagtagtg agagggtggg
     6121 cttgagagaa tttgaggtgt gttcgtgatg tgtggatctt atcgggggct cggttgagtt
     6181 tttggtttgg tgattttgta ggggtgaagg gcggtggaag ggttgctgtg tgcgtgccat
     6241 tccgtgaagc gtcattccta tgtccgacac tgaaatgcgt ccgcgattgt gtggattgtg
     6301 tggaagtgtt tgggcgacga ctgaattgag aaggggagtc gaaacggtgc gtggatgccg
     6361 tgtttgtgct acacacaagc aaaggcaaca acgcaagcgc atcgagccag acgaaccaac
     6421 ccaatattca cacaactgta cgtggcagcg cgctgtatcg ttggaaagac gatgcgtgtg
     6481 cgcgtgccac caccatgtac tagtttccag cctaactgct tgtatacctt gtcttttgcg
     6541 ttgtgcctgc gcgtgctgtg tacctctgct cgtgtgtccc ttcggtctca cgtagctagc
     6601 gggccttcga gttttttttc cttttcccca tttttttcaa cttccagact tcaattacac
     6661 caaaaaataa aattcacaaa cttccggagg ccacctgggc ccaagcttcg attggtacca
     6721 tccaattgat atctagacgg atccagtcga cgattggccg ggctggccag atctccatcg
     6781 ataggacgtc gaggagtact aaggtagact cgtgccgcgc gctgatgatg taggtgcacg
     6841 cgtgcgtgtg ctgcagcgga gccgccgcca ccgcgactgt gtgtgtgtgt gcgcgcgtga
     6901 cgaccggctc gaggaaggag gagcgcgcgt gtgcgtgcga tgcgcctcct cttttttttt
     6961 tttcgccctc gtcggttttt tttttttctg agaggacggc ggcggcggat ggcggggcgg
     7021 cggcgctgac ggagcggcca cgctctgcac ctccccgccc tccgccgtct ctccgttcgc
     7081 cgtcctctcc ttcctcgtgc gtgcgtctgc cgcgcatgca cacgcgcgcg cgttgccgta
     7141 gcgctgtgta tctgtggagg agggccttgt ctctccctcc ccgtcgctga gagatgaagg
     7201 ggcaaaaaga gagagcagtg cgcatgtgtg cctccgctcc ctggagctcc ccacgagccc
     7261 cctgcgccac gcacctgcga acctgcacag cggcccacct gcccgcacac gtgagcagaa
     7321 atgggtgtgt ggagcgggtg atgtcggcgc gcacacgcgc gcgcgctgac cactccgacg
     7381 tgaaaacacg gaagagcgag gctggcaagg cagggcagga acctgatcac gtgcgcgcgc
     7441 acaaacggaa tgagcagctc ctgaagagga gatggggctc atgcacgcag acacgcacgt
     7501 gcacactcct ccgtcttcac ggagcgcggc cctgctgctg ctgccatgtg ccggacgtct
     7561 gccacgcgtc tcttccccac accttgctct tcccccccct tccctcccgg cgaacacaaa
     7621 acacgcgcac gcccaccgaa acaacaacgc gcagctcttc gctctcgttc ttcgagcttg
     7681 gcgcgg
//
