LOCUS       40924_37528        8437 bp DNA     circular SYN 18-DEC-2018
DEFINITION  Cloning vector pRP-LA-GFP-N, complete sequence.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 8437)
  AUTHORS   Soysa R, Tran KD, Ullman B, Yates PA.
  TITLE     Integrating ribosomal promoter vectors that offer a choice of 
            constitutive expression profiles in Leishmania donovani
  JOURNAL   Mol. Biochem. Parasitol. (2016) In press
  PUBMED    26844641
REFERENCE   2  (bases 1 to 8437)
  AUTHORS   Yates PA, Ullman B, Soysa R.
  TITLE     Direct Submission
  JOURNAL   
REFERENCE   3  (bases 1 to 8437)
  TITLE     Direct Submission
REFERENCE   4  (bases 1 to 8437)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Mol. 
            Biochem. Parasitol. (2016) In press"
COMMENT     SGRef: number: 2; type: "Journal Article"; journalName: "Submitted 
            (17-DEC-2015) Biochemistry "
COMMENT     SGRef: number: 3; type: "Journal Article"
COMMENT     ##Assembly-Data-START##
            Sequencing Technology :: Sanger dideoxy sequencing 
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..8437
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     CDS             981..1781
                     /label=NeoR/KanR
                     /note="aminoglycoside phosphotransferase from Tn5"
     polyA_signal    1791..1839
                     /label=HSV TK poly(A) signal
                     /note="herpes simplex virus thymidine kinase
                     polyadenylation signal (Cole and Stacy, 1985)"
     CDS             complement(1962..1973)
                     /label=Factor Xa site
                     /note="Factor Xa recognition and cleavage site"
     rep_origin      complement(3462..4050)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     CDS             complement(4224..5081)
                     /label=AmpR
                     /note="beta-lactamase"
     promoter        complement(5082..5186)
                     /label=AmpR promoter
     misc_feature    5356..6003
                     /note="derived from Leishmania donovani ribosomal RNA
                     spacer region"
     regulatory      6030..6579
                     /note="derived from Leishmania donovani ribosomal RNA
                     genes"
                     /regulatory_class="promoter"
     misc_feature    6601..6682
                     /note="contains artificial trans-splice signal and 5-UTR; 
                     derived from Trypanosoma brucei procyclin gene; TbPARP"
     CDS             6691..7407
                     /label=EGFP
                     /note="enhanced GFP"
     misc_feature    7435..7527
                     /label=multiple cloning site
                     /note="multiple cloning site"
     misc_feature    7538..8422
                     /note="derived from Leishmania major alpha tubulin
                     intergenic region; LmTub"
ORIGIN
        1 cctgcaggga gagaatggtt gaccatgaga cgccgatggg cgatgcgcca gcgcgtgctt
       61 gcgtacttca atcgtttttc atcttgattc agagctaagt agtagcacac cacagcaagc
      121 gtaatcatgt agcatgtatg caggtgcacg agtaggcact ctctgtctct gtccggcatc
      181 tatgatgctc tccggccgcg tgcgcgcgac gcctctgtgt gagtgctcgt gtgagtgtgt
      241 gagagctgtc caggcctttc ctctgcctac tcgtcccacg ctcaggcagg cacagaggaa
      301 tgaaaaggaa gacgaggacg caacgccagg cgcatcgaca tcgcgcacgt gtttgcggtg
      361 tactgtgcgt cgatgatcga gcggccaagc gtccagcttc acgcgaggag gactgcatcg
      421 ctgctcctgt gcgcgtgcgt gcacgcgtca aggcacgctc gattcgttcc cagggtggat
      481 accctccccc ctcccccagc tgcagacaac ccccaccctc tcgaggcttg cacccaggct
      541 cgtcagtctc tctaccactt tctgccttct gtagtgcacc ccacccctgc attctcctct
      601 ctctcacaca cacacacaca cacacacaca cacacacaca cacacgtaca cacgtacaca
      661 cacacgtgcg catgcttgac gcatacgcga cgaattcgaa agctcacctc attcctccct
      721 cctcacacca tcatcggcat ccatagagac acgtgcgcgt agaacgatac agctcacgcg
      781 cacagagagg cgtgttctgg tcgtgcgcat catcggcaca gcactggcgg acgaaactcg
      841 cacacaggca cgccgcctcc tttcacccgt catagatagt tgaattagac gccctcctcc
      901 tccctcatca tcgccgtcgt catccgggtc cgagcactag tcgcgtgtgg cctcgaacac
      961 cgagcgaccc tgcagccaat atgggatcgg ccattgaaca agatggattg cacgcaggtt
     1021 ctccggccgc ttgggtggag aggctattcg gctatgactg ggcacaacag acaatcggct
     1081 gctctgatgc cgccgtgttc cggctgtcag cgcaggggcg cccggttctt tttgtcaaga
     1141 ccgacctgtc cggtgccctg aatgaactgc aggacgaggc agcgcggcta tcgtggctgg
     1201 ccacgacggg cgttccttgc gcagctgtgc tcgacgttgt cactgaagcg ggaagggact
     1261 ggctgctatt gggcgaagtg ccggggcagg atctcctgtc atctcacctt gctcctgccg
     1321 agaaagtatc catcatggct gatgcaatgc ggcggctgca tacgcttgat ccggctacct
     1381 gcccattcga ccaccaagcg aaacatcgca tcgagcgagc acgtactcgg atggaagccg
     1441 gtcttgtcga tcaggatgat ctggacgaag agcatcaggg gctcgcgcca gccgaactgt
     1501 tcgccaggct caaggcgcgc atgcccgacg gcgaggatct cgtcgtgacc catggcgatg
     1561 cctgcttgcc gaatatcatg gtggaaaatg gccgcttttc tggattcatc gactgtggcc
     1621 ggctgggtgt ggcggaccgc tatcaggaca tagcgttggc tacccgtgat attgctgaag
     1681 agcttggcgg cgaatgggct gaccgcttcc tcgtgcttta cggtatcgcc gctcccgatt
     1741 cgcagcgcat cgccttctat cgccttcttg acgagttctt ctgaggggat cggcaataaa
     1801 aagacagaat aaaacgcacg ggtgttgggt cgtttgttcg gatcactagt gatcccgccc
     1861 gcacccgcgc gcgctcaggc cgcgtgtcgt cgtgctcttt ccattttttt tttgtgtgtg
     1921 gtggtgattt gagctgctct ccgttgtgtg ctggggaccc tccttccctc gatctcctcg
     1981 tgcggggtct ccgacgcgca gacgcggtgc gtgcgagcgt gcacgtgctg tcgccgtggc
     2041 tgtagttgcg agcggagaga gagagagagg gagggaggga gggcagaggg cagaggacat
     2101 gcgggtggga acgtgcaccg gcctcgtctc acgcagctgg agcccacgaa tgccaccacc
     2161 acacccttct tccccccccc tccctcttct tcccacggcg gcgacgacga cgggggctca
     2221 gctcacgctt ctgtagggta ttatattaaa gcacatgtgc gtgctgtgct tcggcttctt
     2281 gttggtggtc gttcgctttc aagtccacga ctcctgccgt tgctcaccgc cgggctgtct
     2341 tctttcgccc ctccctcggt gcctcttccg ccgccgcacg cgtgcgctga tcacgcgtct
     2401 tgcgtgatgt gcctgtgtgt atacacaccg tgcacggaga gagcgagcga gcgagagaga
     2461 gagagggcga gaggaagaat ggccggtgcc tcgcgtgggc aagcgtgcgc gagtttgtcg
     2521 tgtcgctgcg ccgcgtggat gtcgcgcagc gcaagacccc gtgcgcctga aacgtgaagg
     2581 gaggtcgaga agcgtgccgc atgaggcctt aaggcaggaa agtgaaaaga gctcgacgcg
     2641 agtgcagcac gcgcgacatc gaacaggacg gaatagacac tcagcccctt cccctttcaa
     2701 aaactgaatg gacggacatc gtaacgcgct tctctcccct ccactccccc tgtccctgtc
     2761 tccttgacgt gtgctgctct cgtgagcgac agatgaagcg aggcgtagaa ggaaaggggg
     2821 gggagcgagg cctgcacaca cacacacaca cacacacaca cacacacaca cacacacaca
     2881 cacacacaca cgaaaagcaa gtttgaggca gacgtggttt cacacgattc ctgtgcaaga
     2941 agcgtgcctg cgtgacggtg tggctgtgta tgcatgcgtg aagggttacc gcaacgatgc
     3001 gcaatggact cccccgcttt ccatttcgtc accttccgcc tctctctctc tctctctcac
     3061 catctacgcg tgcacatcat caactgtctc ttgtcggtgc tcaccaccct caaccacccc
     3121 tcactttcaa ggcttcccga acgcacacaa aaggcgtgaa aaccgctcgc gtgtgttgag
     3181 ccgtccaccg gcgcgccggc cgcggatcga aacctgtcgt gccagctgca ttaatgaatc
     3241 ggccaacgcg cggggagagg cggtttgcgt attgggcgct cttccgcttc ctcgctcact
     3301 gactcgctgc gctcggtcgt tcggctgcgg cgagcggtat cagctcactc aaaggcggta
     3361 atacggttat ccacagaatc aggggataac gcaggaaaga acatgtgagc aaaaggccag
     3421 caaaaggcca ggaaccgtaa aaaggccgcg ttgctggcgt ttttccatag gctccgcccc
     3481 cctgacgagc atcacaaaaa tcgacgctca agtcagaggt ggcgaaaccc gacaggacta
     3541 taaagatacc aggcgtttcc ccctggaagc tccctcgtgc gctctcctgt tccgaccctg
     3601 ccgcttaccg gatacctgtc cgcctttctc ccttcgggaa gcgtggcgct ttctcatagc
     3661 tcacgctgta ggtatctcag ttcggtgtag gtcgttcgct ccaagctggg ctgtgtgcac
     3721 gaaccccccg ttcagcccga ccgctgcgcc ttatccggta actatcgtct tgagtccaac
     3781 ccggtaagac acgacttatc gccactggca gcagccactg gtaacaggat tagcagagcg
     3841 aggtatgtag gcggtgctac agagttcttg aagtggtggc ctaactacgg ctacactaga
     3901 aggacagtat ttggtatctg cgctctgctg aagccagtta ccttcggaaa aagagttggt
     3961 agctcttgat ccggcaaaca aaccaccgct ggtagcggtg gtttttttgt ttgcaagcag
     4021 cagattacgc gcagaaaaaa aggatctcaa gaagatcctt tgatcttttc tacggggtct
     4081 gacgctcagt ggaacgaaaa ctcacgttaa gggattttgg tcatgagatt atcaaaaagg
     4141 atcttcacct agatcctttt aaattaaaaa tgaagtttta aatcaatcta aagtatatat
     4201 gagtaaactt ggtctgacag ttaccaatgc ttaatcagtg aggcacctat ctcagcgatc
     4261 tgtctatttc gttcatccat agttgcctga ctccccgtcg tgtagataac tacgatacgg
     4321 gagggcttac catctggccc cagtgctgca atgataccgc gagacccacg ctcaccggct
     4381 ccagatttat cagcaataaa ccagccagcc ggaagggccg agcgcagaag tggtcctgca
     4441 actttatccg cctccatcca gtctattaat tgttgccggg aagctagagt aagtagttcg
     4501 ccagttaata gtttgcgcaa cgttgttgcc attgctacag gcatcgtggt gtcacgctcg
     4561 tcgtttggta tggcttcatt cagctccggt tcccaacgat caaggcgagt tacatgatcc
     4621 cccatgttgt gcaaaaaagc ggttagctcc ttcggtcctc cgatcgttgt cagaagtaag
     4681 ttggccgcag tgttatcact catggttatg gcagcactgc ataattctct tactgtcatg
     4741 ccatccgtaa gatgcttttc tgtgactggt gagtactcaa ccaagtcatt ctgagaatag
     4801 tgtatgcggc gaccgagttg ctcttgcccg gcgtcaatac gggataatac cgcgccacat
     4861 agcagaactt taaaagtgct catcattgga aaacgttctt cggggcgaaa actctcaagg
     4921 atcttaccgc tgttgagatc cagttcgatg taacccactc gtgcacccaa ctgatcttca
     4981 gcatctttta ctttcaccag cgtttctggg tgagcaaaaa caggaaggca aaatgccgca
     5041 aaaaagggaa taagggcgac acggaaatgt tgaatactca tactcttcct ttttcaatat
     5101 tattgaagca tttatcaggg ttattgtctc atgagcggat acatatttga atgtatttag
     5161 aaaaataaac aaataggggt tccgcgcaca tttccccgaa aagtgccacc taaattgtaa
     5221 gcgttaatat tttgttaaaa ttcgcgttaa atttttgtta aatcagctca ttttttaacc
     5281 aataggccga aatcggcaaa atcccttata aatcaaaaga atagaccgag atagggttga
     5341 gtgttgttcc acacaaaata taggaggggg gggggtgaat acgttatata tgtataatgt
     5401 gtgtcgctta gcttcacaaa cccagaagga acggtgcaaa ggacccgaca accagctgtg
     5461 tgcgcgtata tatttacgtc tgcatacacg gctcgttgtc gggtccttgc ccttttccgt
     5521 ccccttttcc gtcctgcgcg tgtgtgtatg ggcgcgggtg gggtttgtgc atgcgggggg
     5581 gggggaggat cgagcacccc ccaacactac ctccatccaa aacaaacaca cagcagagag
     5641 tgcccgccaa ccttgggcgc ttctcggttg ccgtgtgtgt cattgccctg cagagagggc
     5701 ggaacagggg ggtggaggtg ggaatagggg ggggggcacg atttccaccg ccaaagtctc
     5761 tccctacggg aacacgccaa taggcatgcc ctttgactgt aagttacagt caagcaatac
     5821 ggcggcttac ctccgccacc cgacccggca accagctgcg cccaaccgcg cggctcgttg
     5881 tcgggtcctt gccctttttt cactgcgacc ctcaaagaca gtgtacgcca gtccaacgat
     5941 cccactccca gcgatggacc gcgcccaacg ctcacccgca acccacattc ggaattcgac
     6001 atcatgccct aggatttaaa tgcggccgcg aggtctgcga ttgacgtagg agttgcaagg
     6061 gggagggggt atgaacgggg tgggtagagc ttttttaggt ggaagtagtg agagggtggg
     6121 cttgagagaa tttgaggtgt gttcgtgatg tgtggatctt atcgggggct cggttgagtt
     6181 tttggtttgg tgattttgta ggggtgaagg gcggtggaag ggttgctgtg tgcgtgccat
     6241 tccgtgaagc gtcattccta tgtccgacac tgaaatgcgt ccgcgattgt gtggattgtg
     6301 tggaagtgtt tgggcgacga ctgaattgag aaggggagtc gaaacggtgc gtggatgccg
     6361 tgtttgtgct acacacaagc aaaggcaaca acgcaagcgc atcgagccag acgaaccaac
     6421 ccaatattca cacaactgta cgtggcagcg cgctgtatcg ttggaaagac gatgcgtgtg
     6481 cgcgtgccac caccatgtac tagtttccag cctaactgct tgtatacctt gtcttttgcg
     6541 ttgtgcctgc gcgtgctgtg tacctctgct cgtgtgtccc ttcggtctca cgtagctagc
     6601 gggccttcga gttttttttc cttttcccca tttttttcaa cttgaagact tcaattacac
     6661 caaaaaataa aattcacaaa cttccggacc atggtgagca agggcgagga gctgttcacg
     6721 ggcgtggtgc cgatcctggt ggagctggac ggcgacgtga acggccacaa gttcagcgtg
     6781 agcggcgagg gcgagggcga cgcgacgtac ggcaagctga cgctgaagtt catctgcacg
     6841 acgggcaagc tgccggtgcc gtggccgacg ctggtgacga cgctgacgta cggcgtgcag
     6901 tgcttcagcc gctacccgga ccacatgaag cagcacgact tcttcaagag cgcgatgccg
     6961 gagggctacg tgcaggagcg cacgatcttc ttcaaggacg acggcaacta caagacgcgc
     7021 gcggaggtga agttcgaggg cgacacgctg gtgaaccgca tcgagctgaa gggcatcgac
     7081 ttcaaggagg acggcaacat cctgggccac aagctggagt acaactacaa cagccacaac
     7141 gtgtacatca tggcggacaa gcagaagaac ggcatcaagg tgaacttcaa gatccgccac
     7201 aacatcgagg acggcagcgt gcagctggcg gaccactacc agcagaacac gccgatcggc
     7261 gacggcccgg tgctgctgcc ggacaaccac tacctgagca cgcagagcgc gctgagcaag
     7321 gacccgaacg agaagcgcga ccacatggtg ctgctggagt tcgtgacggc ggcgggcatc
     7381 acgctgggca tggacgagct gtacaagggt ggcggtagcg gcggtggcca tatgccggag
     7441 gccacctggg cccaagcttc gattggtacc atccaattga tatctagacg gatccagtcg
     7501 acgattggcc gggctggcca gatctccatc gataggacgt cgaggagtac taaggtagac
     7561 tcgtgccgcg cgctgatgat gtaggtgcac gcgtgcgtgt gctgcagcgg agccgccgcc
     7621 accgcgactg tgtgtgtgtg tgcgcgcgtg acgaccggct cgaggaagga ggagcgcgcg
     7681 tgtgcgtgcg atgcgcctcc tctttttttt ttttcgccct cgtcggtttt ttttttttct
     7741 gagaggacgg cggcggcgga tggcggggcg gcggcgctga cggagcggcc acgctctgca
     7801 cctccccgcc ctccgccgtc tctccgttcg ccgtcctctc cttcctcgtg cgtgcgtctg
     7861 ccgcgcatgc acacgcgcgc gcgttgccgt agcgctgtgt atctgtggag gagggccttg
     7921 tctctccctc cccgtcgctg agagatgaag gggcaaaaag agagagcagt gcgcatgtgt
     7981 gcctccgctc cctggagctc cccacgagcc ccctgcgcca cgcacctgcg aacctgcaca
     8041 gcggcccacc tgcccgcaca cgtgagcaga aatgggtgtg tggagcgggt gatgtcggcg
     8101 cgcacacgcg cgcgcgctga ccactccgac gtgaaaacac ggaagagcga ggctggcaag
     8161 gcagggcagg aacctgatca cgtgcgcgcg cacaaacgga atgagcagct cctgaagagg
     8221 agatggggct catgcacgca gacacgcacg tgcacactcc tccgtcttca cggagcgcgg
     8281 ccctgctgct gctgccatgt gccggacgtc tgccacgcgt ctcttcccca caccttgctc
     8341 ttcccccccc ttccctcccg gcgaacacaa aacacgcgca cgcccaccga aacaacaacg
     8401 cgcagctctt cgctctcgtt cttcgagctt ggcgcgg
//
