LOCUS       40924_37483       12097 bp DNA     circular SYN 18-DEC-2018
DEFINITION  Plant RNAi vector pRNAi-LIC, complete sequence.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 12097)
  AUTHORS   Xu G, Sui N, Tang Y, Xie K, Lai Y, Liu Y.
  TITLE     One-step, zero-background ligation-independent cloning 
            intron-containing hairpin RNA constructs for RNAi in plants
  JOURNAL   New Phytol. 187 (1), 240-250 (2010)
  PUBMED    20406406
REFERENCE   2  (bases 1 to 12097)
  AUTHORS   Xu G, Liu Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (04-SEP-2009) Biological Sciences and Biotechnology, 
            Tsinghua University, Beijing 100084, China
REFERENCE   3  (bases 1 to 12097)
  TITLE     Direct Submission
REFERENCE   4  (bases 1 to 12097)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "New 
            Phytol."; date: "2010"; volume: "187"; issue: "1"; pages: "240-250"
COMMENT     SGRef: number: 2; type: "Journal Article"; journalName: "Submitted 
            (04-SEP-2009) Biological Sciences and Biotechnology, Tsinghua 
            University, Beijing 100084, China"
COMMENT     SGRef: number: 3; type: "Journal Article"
FEATURES             Location/Qualifiers
     source          1..12097
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        427..769
                     /label=CaMV 35S promoter
                     /note="strong constitutive promoter from cauliflower mosaic
                     virus"
     misc_feature    852..866
                     /label=adaptor LIC1
                     /note="adaptor LIC1"
     misc_feature    complement(867..882)
                     /label=adaptor LIC3
                     /note="adaptor LIC3"
     CDS             complement(1487..2143)
                     /codon_start=1
                     /label=CmR
                     /note="chloramphenicol acetyltransferase"
                     /translation="MEKKITGYTTVDISQWHRKEHFEAFQSVAQCTYNQTVQLDITAFL
                     KTVKKNKHKFYPAFIHILARLMNAHPEFRMAMKDGELVIWDSVHPCYTVFHEQTETFSS
                     LWSEYHDDFRQFLHIYSQDVACYGENLAYFPKGFIENMFFVSANPWVSFTSFDLNVANM
                     DNFFAPVFTMGKYYTQGDKVLMPLAIQVHHAVCDGFHVGRMLNELQQYCDEWQGGA"
     misc_feature    2497..2507
                     /label=adaptor LIC2
                     /note="adaptor LIC2"
     promoter        2659..2761
                     /label=cat promoter
                     /note="promoter of the E. coli cat gene encoding
                     chloramphenicol acetyltransferase"
     CDS             2792..3094
                     /codon_start=1
                     /label=ccdB
                     /note="CcdB, a bacterial toxin that poisons DNA gyrase"
                     /translation="MQFKVYTYKRESRYRLFVDVQSDIIDTPGRRMVIPLASARLLSDK
                     VSRELYPVVHIGDESWRMMTTDMASVPVSVIGEEVADLSHRENDIKNAINLMFWGI"
     misc_feature    complement(3122..3136)
                     /label=adaptor LIC4
                     /note="adaptor LIC4"
     terminator      3150..3402
                     /label=NOS terminator
                     /note="nopaline synthase terminator and poly(A) signal"
     primer_bind     complement(3422..3438)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     protein_bind    3446..3462
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(3470..3500)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    complement(3515..3536)
                     /label=CAP binding site
                     /note="CAP binding activates transcription in the presence
                     of cAMP."
     promoter        3727..4404
                     /label=CaMV 35S promoter (enhanced)
                     /note="cauliflower mosaic virus 35S promoter with a
                     duplicated enhancer region"
     CDS             4473..5261
                     /codon_start=1
                     /label=NeoR/KanR
                     /note="aminoglycoside phosphotransferase"
                     /translation="IEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRPV
                     LFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLSS
                     HLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQG
                     LAPAELFARLKARMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIAL
                     ATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     polyA_signal    5321..5495
                     /label=CaMV poly(A) signal
                     /note="cauliflower mosaic virus polyadenylation signal"
     misc_feature    complement(5573..5597)
                     /label=LB T-DNA repeat
                     /note="left border repeat from nopaline C58 T-DNA"
     CDS             6022..6813
                     /codon_start=1
                     /label=KanR
                     /note="aminoglycoside phosphotransferase"
                     /translation="MAKMRISPELKKLIEKYRCVKDTEGMSPAKVYKLVGENENLYLKM
                     TDSRYKGTTYDVEREKDMMLWLEGKLPVPKVLHFERHDGWSNLLMSEADGVLCSEEYED
                     EQSPEKIIELYAECIRLFHSIDISDCPYTNSLDSRLAELDYLLNNDLADVDCENWEEDT
                     PFKDPRELYDFLKTEKPEEELVFSHGDLGDSNIFVKDGKVSGFIDLGRSGRADKWYDIA
                     FCVRSIREDIGEEQYVELFFDLLGIKPDWEKIKYYILLDELF"
     rep_origin      6903..7491
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     misc_feature    complement(7677..7817)
                     /label=bom
                     /note="basis of mobility region from pBR322"
     rep_origin      complement(8161..8355)
                     /direction=LEFT
                     /label=pVS1 oriV
                     /note="origin of replication for the Pseudomonas plasmid
                     pVS1 (Heeb et al., 2000)"
     CDS             complement(8424..9488)
                     /codon_start=1
                     /label=pVS1 RepA
                     /note="replication protein from the Pseudomonas plasmid
                     pVS1 (Heeb et al., 2000)"
                     /translation="GRKPSGPVQIGAALGDDLVEKLKAAQAAQRQRIEAEARPGESWQA
                     AADRIRKESRQPPAAGAPSIRKPPKGDEQPDFFVPMLYDVGTRDSRSIMDVAVFRLSKR
                     DRRAGEVIRYELPDGHVEVSAGPAGMASVWDYDLVLMAVSHLTESMNRYREGKGDKPGR
                     VFRPHVADVLKFCRRADGGKQKDDLVETCIRLNTTHVAMQRTKKAKNGRLVTVSEGEAL
                     ISRYKIVKSETGRPEYIEIELADWMYREITEGKNPDVLTVHPDYFLIDPGIGRFLYRLA
                     RRAAGKAEARWLFKTIYERSGSAGEFKKFCFTVRKLIGSNDLPEYDLKEEAGQAGPILV
                     MRYRNLIEGEASAGS"
     CDS             complement(9925..10551)
                     /codon_start=1
                     /label=pVS1 StaA
                     /note="stability protein from the Pseudomonas plasmid pVS1
                     (Heeb et al., 2000)"
                     /translation="MKVIAVLNQKGGSGKTTIATHLARALQLAGADVLLVDSDPQGSAR
                     DWAAVREDQPLTVVGIDRPTIDRDVKAIGRRDFVVIDGAPQAADLAVSAIKAADFVLIP
                     VQPSPYDIWATADLVELVKQRIEVTDGRLQAAFVVSRAIKGTRIGGEVAEALAGYELPI
                     LESRITQRVSYPGTAAAGTTVLESEPEGDAAREVQALAAEIKSKLI"
     misc_feature    complement(11851..11875)
                     /label=RB T-DNA repeat
                     /note="right border repeat from nopaline C58 T-DNA"
     primer_bind     12078..12094
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
ORIGIN
        1 aagcttgcat gcctgcaggt caacatggtg gagcacgaca ctctcgtcta ctccaagaat
       61 atcaaagata cagtctcaga agaccagagg gctattgaga cttttcaaca aagggtaata
      121 tcgggaaacc tcctcggatt ccattgccca gctatctgtc acttcatcga aaggacagta
      181 gaaaaggaag atggcttcta caaatgccat cattgcgata aaggaaaggc tatcgttcaa
      241 gatgcctcta ccgacagtgg tcccaaagat ggacccccac ccacgaggaa catcgtggaa
      301 aaagaagacg ttccaaccac gtcttcaaag caagtggatt gatgtgatgg tcaacatggt
      361 ggagcacgac actctcgtct actccaagaa tatcaaagat acagtctcag aagaccagag
      421 ggctattgag acttttcaac aaagggtaat atcgggaaac ctcctcggat tccattgccc
      481 agctatctgt cacttcatcg aaaggacagt agaaaaggaa gatggcttct acaaatgcca
      541 tcattgcgat aaaggaaagg ctatcgttca agatgcctct accgacagtg gtcccaaaga
      601 tggaccccca cccacgagga acatcgtgga aaaagaagac gttccaacca cgtcttcaaa
      661 gcaagtggat tgatgtgata tctccactga cgtaagggat gacgcacaat cccactatcc
      721 ttcgcaagac ccttcctcta tataaggaag ttcatttcat ttggagaggg ggccctctag
      781 atgcatgctc gagcggccgc cagtgtgatg gatatctgca gaattgccct cggggatccg
      841 gggatccgag ctcgacgaca agacccgggt tccgtgctgg aaccaactgt aatcaatcca
      901 aatgtaagat caatgataac acaatgacat gatctatcat gttaccttgt ttattcatgt
      961 tcgactaatt catttaatta atagtcaatc catttagaag ttaataaaac tacaagtatt
     1021 atttagaaat taataagaat gttgattgaa aataatacta tataaaatga tagatcttgc
     1081 gctttgttat attagcatta gattatgttt tgttacatta gattactgtt tctattagtt
     1141 tgatattatt tgttacttta gcttgttatt taatattttg tttattgata aattacaagc
     1201 agattggaat ttctaacaaa atatttatta acttttaaac taaaatattt agtaatggta
     1261 tagatattta attatataat aaactattaa tcataaaaaa atattatttt aatttattta
     1321 ttcttatttt tactatagta ttttatcatt gatatttaat tcatcaaacc agctagaatt
     1381 actattatgg gaccgacata tcagtatata ttcttatacc gcaaaaatca gcgcgcaaat
     1441 acgcatactg ttatctggct tttagtaagc cggatccacg cgattacgcc ccgccctgcc
     1501 actcatcgca gtactgttgt aattcattaa gcattctgcc gacatggaag ccatcacaga
     1561 cggcatgatg aacctgaatc gccagcggca tcagcacctt gtcgccttgc gtataatatt
     1621 tgcccatggt gaaaacgggg gcgaagaagt tgtccatatt ggccacgttt aaatcaaaac
     1681 tggtgaaact cacccaggga ttggctgaga cgaaaaacat attctcaata aaccctttag
     1741 ggaaataggc caggttttca ccgtaacacg ccacatcttg cgaatatatg tgtagaaact
     1801 gccggaaatc gtcgtggtat tcactccaga gcgatgaaaa cgtttcagtt tgctcatgga
     1861 aaacggtgta acaagggtga acactatccc atatcaccag ctcaccgtct ttcattgcca
     1921 tacggaattc cggatgagca ttcatcaggc gggcaagaat gtgaataaag gccggataaa
     1981 acttgtgctt atttttcttt acggtcttta aaaaggccgt aatatccagc tgaacggtct
     2041 ggttataggt acattgagca actgactgaa atgcctcaaa atgttcttta cgatgccatt
     2101 gggatatatc aacggtggta tatccagtga tttttttctc cattttagct tccttagctc
     2161 ctgaaaatct cgataactca aaaaatacgc ccggtagtga tcttatttca ttatggtgaa
     2221 agttggaacc tcttaccggc cgtatatcat cttacatgtt cgatcaaatt cattaaaaat
     2281 aatatactta ctctcaactt ttatcttctt cgtcttacac atcacttgtc atattttttt
     2341 acattactat gttgtttatg taaacaatat atttataaat tattttttca caattataac
     2401 aactatatta ttataatcat actaattaac atcacttaac tattttatac taaaaggaaa
     2461 aaagaaaata attatttcct taccaagggc cctgaggaga agagcccggg aattctcgac
     2521 taagttggca gcatcacccg acgcactttg cgccgaataa atacctgtga cggaagatca
     2581 cttcgcagaa taaataaatc ctggtgtccc tgttgatacc gggaagccct gggccaactt
     2641 ttggcgaaaa tgagacgttg atcggcacgt aagaggttcc aactttcacc ataatgaaat
     2701 aagatcacta ccgggcgtat tttttgagtt atcgagattt tcaggagcta aggaagctaa
     2761 acttttgctg acgagaacag ggactggtga aatgcagttt aaggtttaca cctataaaag
     2821 agagagccgt tatcgtctgt ttgtggatgt acagagtgat attattgaca cgcctgggcg
     2881 acggatggtg atccccctgg ccagtgcacg tctgctgtca gataaagtct cccgtgaact
     2941 ttacccggtg gtgcatatcg gggatgaaag ctggcgcatg atgaccaccg atatggccag
     3001 tgtgccggtc tccgttatcg gggaagaagt ggctgatctc agccaccgcg aaaatgacat
     3061 caaaaacgcc attaacctga tgttctgggg aatataagtc aggctccctt atacacagcc
     3121 cgggtcgtgt gctctagagc tcggaaaaag atcgttcaaa catttggcaa taaagtttct
     3181 taagattgaa tcctgttgcc ggtcttgcga tgattatcat ataatttctg ttgaattacg
     3241 ttaagcatgt aataattaac atgtaatgca tgacgttatt tatgagatgg gtttttatga
     3301 ttagagtccc gcaattatac atttaatacg cgatagaaaa caaaatatag cgcgcaaact
     3361 aggataaatt atcgcgcgcg gtgtcatcta tgttactaga tcaaaagaat tcgtaatcat
     3421 ggtcatagct gtttcctgtg tgaaattgtt atccgctcac aattccacac aacatacgag
     3481 ccggaagcat aaagtgtaaa gcctggggtg cctaatgagt gagctaactc acattaattg
     3541 cgttgcgctc actgcccgct ttccagtcgg gaaacctgtc gtgccagctg cattaatgaa
     3601 tcggccaacg cgcggggaga ggcggtttgc gtattggcta gagcagcttg ccaacatggt
     3661 ggagcacgac actctcgtct actccaagaa tatcaaagat acagtctcag aagaccaaag
     3721 ggctattgag acttttcaac aaagggtaat atcgggaaac ctcctcggat tccattgccc
     3781 agctatctgt cacttcatca aaaggacagt agaaaaggaa ggtggcacct acaaatgcca
     3841 tcattgcgat aaaggaaagg ctatcgttca agatgcctct gccgacagtg gtcccaaaga
     3901 tggaccccca cccacgagga gcatcgtgga aaaagaagac gttccaacca cgtcttcaaa
     3961 gcaagtggat tgatgtgata acatggtgga gcacgacact ctcgtctact ccaagaatat
     4021 caaagataca gtctcagaag accaaagggc tattgagact tttcaacaaa gggtaatatc
     4081 gggaaacctc ctcggattcc attgcccagc tatctgtcac ttcatcaaaa ggacagtaga
     4141 aaaggaaggt ggcacctaca aatgccatca ttgcgataaa ggaaaggcta tcgttcaaga
     4201 tgcctctgcc gacagtggtc ccaaagatgg acccccaccc acgaggagca tcgtggaaaa
     4261 agaagacgtt ccaaccacgt cttcaaagca agtggattga tgtgatatct ccactgacgt
     4321 aagggatgac gcacaatccc actatccttc gcaagacctt cctctatata aggaagttca
     4381 tttcatttgg agaggacacg ctgaaatcac cagtctctct ctacaaatct atctctctcg
     4441 agctttcgca gatctgtcga tcgaccatgg ggattgaaca agatggattg cacgcaggtt
     4501 ctccggccgc ttgggtggag aggctattcg gctatgactg ggcacaacag acaatcggct
     4561 gctctgatgc cgccgtgttc cggctgtcag cgcaggggcg cccggttctt tttgtcaaga
     4621 ccgacctgtc cggtgccctg aatgaactcc aggacgaggc agcgcggcta tcgtggctgg
     4681 ccacgacggg cgttccttgc gcagctgtgc tcgacgttgt cactgaagcg ggaagggact
     4741 ggctgctatt gggcgaagtg ccggggcagg atctcctgtc atctcacctt gctcctgccg
     4801 agaaagtatc catcatggct gatgcaatgc ggcggctgca tacgcttgat ccggctacct
     4861 gcccattcga ccaccaagcg aaacatcgca tcgagcgagc acgtactcgg atggaagccg
     4921 gtcttgtcga tcaggatgat ctggacgaag agcatcaggg gctcgcgcca gccgaactgt
     4981 tcgccaggct caaggcgcgc atgcccgacg gcgaggatct cgtcgtgaca catggcgatg
     5041 cctgcttgcc gaatatcatg gtggaaaatg gccgcttttc tggattcatc gactgtggcc
     5101 ggctgggtgt ggcggaccgc tatcaggaca tagcgttggc tacccgtgat attgctgaag
     5161 agcttggcgg cgaatgggct gaccgcttcc tcgtgcttta cggtatcgcc gctcccgatt
     5221 cgcagcgcat cgccttctat cgccttcttg acgagttctt ctgagcggga ctctggggtt
     5281 cggatcgatc ctctagctag agtcgatcga caagctcgag tttctccata ataatgtgtg
     5341 agtagttccc agataaggga attagggttc ctatagggtt tcgctcatgt gttgagcata
     5401 taagaaaccc ttagtatgta tttgtatttg taaaatactt ctatcaataa aatttctaat
     5461 tcctaaaacc aaaatccagt actaaaatcc agatcccccg aattaattcg gcgttaattc
     5521 agtacattaa aaacgtccgc aatgtgttat taagttgtct aagcgtcaat ttgtttacac
     5581 cacaatatat cctgccacca gccagccaac agctccccga ccggcagctc ggcacaaaat
     5641 caccactcga tacaggcagc ccatcagtcc gggacggcgt cagcgggaga gccgttgtaa
     5701 ggcggcagac tttgctcatg ttaccgatgc tattcggaag aacggcaact aagctgccgg
     5761 gtttgaaaca cggatgatct cgcggagggt agcatgttga ttgtaacgat gacagagcgt
     5821 tgctgcctgt gatcaccgcg gtttcaaaat cggctccgtc gatactatgt tatacgccaa
     5881 ctttgaaaac aactttgaaa aagctgtttt ctggtattta aggttttaga atgcaaggaa
     5941 cagtgaattg gagttcgtct tgttataatt agcttcttgg ggtatcttta aatactgtag
     6001 aaaagaggaa ggaaataata aatggctaaa atgagaatat caccggaatt gaaaaaactg
     6061 atcgaaaaat accgctgcgt aaaagatacg gaaggaatgt ctcctgctaa ggtatataag
     6121 ctggtgggag aaaatgaaaa cctatattta aaaatgacgg acagccggta taaagggacc
     6181 acctatgatg tggaacggga aaaggacatg atgctatggc tggaaggaaa gctgcctgtt
     6241 ccaaaggtcc tgcactttga acggcatgat ggctggagca atctgctcat gagtgaggcc
     6301 gatggcgtcc tttgctcgga agagtatgaa gatgaacaaa gccctgaaaa gattatcgag
     6361 ctgtatgcgg agtgcatcag gctctttcac tccatcgaca tatcggattg tccctatacg
     6421 aatagcttag acagccgctt agccgaattg gattacttac tgaataacga tctggccgat
     6481 gtggattgcg aaaactggga agaagacact ccatttaaag atccgcgcga gctgtatgat
     6541 tttttaaaga cggaaaagcc cgaagaggaa cttgtctttt cccacggcga cctgggagac
     6601 agcaacatct ttgtgaaaga tggcaaagta agtggcttta ttgatcttgg gagaagcggc
     6661 agggcggaca agtggtatga cattgccttc tgcgtccggt cgatcaggga ggatatcggg
     6721 gaagaacagt atgtcgagct attttttgac ttactgggga tcaagcctga ttgggagaaa
     6781 ataaaatatt atattttact ggatgaattg ttttagtacc tagaatgcat gaccaaaatc
     6841 ccttaacgtg agttttcgtt ccactgagcg tcagaccccg tagaaaagat caaaggatct
     6901 tcttgagatc ctttttttct gcgcgtaatc tgctgcttgc aaacaaaaaa accaccgcta
     6961 ccagcggtgg tttgtttgcc ggatcaagag ctaccaactc tttttccgaa ggtaactggc
     7021 ttcagcagag cgcagatacc aaatactgtc cttctagtgt agccgtagtt aggccaccac
     7081 ttcaagaact ctgtagcacc gcctacatac ctcgctctgc taatcctgtt accagtggct
     7141 gctgccagtg gcgataagtc gtgtcttacc gggttggact caagacgata gttaccggat
     7201 aaggcgcagc ggtcgggctg aacggggggt tcgtgcacac agcccagctt ggagcgaacg
     7261 acctacaccg aactgagata cctacagcgt gagctatgag aaagcgccac gcttcccgaa
     7321 gggagaaagg cggacaggta tccggtaagc ggcagggtcg gaacaggaga gcgcacgagg
     7381 gagcttccag ggggaaacgc ctggtatctt tatagtcctg tcgggtttcg ccacctctga
     7441 cttgagcgtc gatttttgtg atgctcgtca ggggggcgga gcctatggaa aaacgccagc
     7501 aacgcggcct ttttacggtt cctggccttt tgctggcctt ttgctcacat gttctttcct
     7561 gcgttatccc ctgattctgt ggataaccgt attaccgcct ttgagtgagc tgataccgct
     7621 cgccgcagcc gaacgaccga gcgcagcgag tcagtgagcg aggaagcgga agagcgcctg
     7681 atgcggtatt ttctccttac gcatctgtgc ggtatttcac accgcatatg gtgcactctc
     7741 agtacaatct gctctgatgc cgcatagtta agccagtata cactccgcta tcgctacgtg
     7801 actgggtcat ggctgcgccc cgacacccgc caacacccgc tgacgcgccc tgacgggctt
     7861 gtctgctccc ggcatccgct tacagacaag ctgtgaccgt ctccgggagc tgcatgtgtc
     7921 agaggttttc accgtcatca ccgaaacgcg cgaggcaggg tgccttgatg tgggcgccgg
     7981 cggtcgagtg gcgacggcgc ggcttgtccg cgccctggta gattgcctgg ccgtaggcca
     8041 gccatttttg agcggccagc ggccgcgata ggccgacgcg aagcggcggg gcgtagggag
     8101 cgcagcgacc gaagggtagg cgctttttgc agctcttcgg ctgtgcgctg gccagacagt
     8161 tatgcacagg ccaggcgggt tttaagagtt ttaataagtt ttaaagagtt ttaggcggaa
     8221 aaatcgcctt ttttctcttt tatatcagtc acttacatgt gtgaccggtt cccaatgtac
     8281 ggctttgggt tcccaatgta cgggttccgg ttcccaatgt acggctttgg gttcccaatg
     8341 tacgtgctat ccacaggaaa gagacctttt cgaccttttt cccctgctag ggcaatttgc
     8401 cctagcatct gctccgtaca ttaggaaccg gcggatgctt cgccctcgat caggttgcgg
     8461 tagcgcatga ctaggatcgg gccagcctgc cccgcctcct ccttcaaatc gtactccggc
     8521 aggtcatttg acccgatcag cttgcgcacg gtgaaacaga acttcttgaa ctctccggcg
     8581 ctgccactgc gttcgtagat cgtcttgaac aaccatctgg cttctgcctt gcctgcggcg
     8641 cggcgtgcca ggcggtagag aaaacggccg atgccgggat cgatcaaaaa gtaatcgggg
     8701 tgaaccgtca gcacgtccgg gttcttgcct tctgtgatct cgcggtacat ccaatcagct
     8761 agctcgatct cgatgtactc cggccgcccg gtttcgctct ttacgatctt gtagcggcta
     8821 atcaaggctt caccctcgga taccgtcacc aggcggccgt tcttggcctt cttcgtacgc
     8881 tgcatggcaa cgtgcgtggt gtttaaccga atgcaggttt ctaccaggtc gtctttctgc
     8941 tttccgccat cggctcgccg gcagaacttg agtacgtccg caacgtgtgg acggaacacg
     9001 cggccgggct tgtctccctt cccttcccgg tatcggttca tggattcggt tagatgggaa
     9061 accgccatca gtaccaggtc gtaatcccac acactggcca tgccggccgg ccctgcggaa
     9121 acctctacgt gcccgtctgg aagctcgtag cggatcacct cgccagctcg tcggtcacgc
     9181 ttcgacagac ggaaaacggc cacgtccatg atgctgcgac tatcgcgggt gcccacgtca
     9241 tagagcatcg gaacgaaaaa atctggttgc tcgtcgccct tgggcggctt cctaatcgac
     9301 ggcgcaccgg ctgccggcgg ttgccgggat tctttgcgga ttcgatcagc ggccgcttgc
     9361 cacgattcac cggggcgtgc ttctgcctcg atgcgttgcc gctgggcggc ctgcgcggcc
     9421 ttcaacttct ccaccaggtc atcacccagc gccgcgccga tttgtaccgg gccggatggt
     9481 ttgcgaccgt cacgccgatt cctcgggctt gggggttcca gtgccattgc agggccggca
     9541 gacaacccag ccgcttacgc ctggccaacc gcccgttcct ccacacatgg ggcattccac
     9601 ggcgtcggtg cctggttgtt cttgattttc catgccgcct cctttagccg ctaaaattca
     9661 tctactcatt tattcatttg ctcatttact ctggtagctg cgcgatgtat tcagatagca
     9721 gctcggtaat ggtcttgcct tggcgtaccg cgtacatctt cagcttggtg tgatcctccg
     9781 ccggcaactg aaagttgacc cgcttcatgg ctggcgtgtc tgccaggctg gccaacgttg
     9841 cagccttgct gctgcgtgcg ctcggacggc cggcacttag cgtgtttgtg cttttgctca
     9901 ttttctcttt acctcattaa ctcaaatgag ttttgattta atttcagcgg ccagcgcctg
     9961 gacctcgcgg gcagcgtcgc cctcgggttc tgattcaaga acggttgtgc cggcggcggc
    10021 agtgcctggg tagctcacgc gctgcgtgat acgggactca agaatgggca gctcgtaccc
    10081 ggccagcgcc tcggcaacct caccgccgat gcgcgtgcct ttgatcgccc gcgacacgac
    10141 aaaggccgct tgtagccttc catccgtgac ctcaatgcgc tgcttaacca gctccaccag
    10201 gtcggcggtg gcccatatgt cgtaagggct tggctgcacc ggaatcagca cgaagtcggc
    10261 tgccttgatc gcggacacag ccaagtccgc cgcctggggc gctccgtcga tcactacgaa
    10321 gtcgcgccgg ccgatggcct tcacgtcgcg gtcaatcgtc gggcggtcga tgccgacaac
    10381 ggttagcggt tgatcttccc gcacggccgc ccaatcgcgg gcactgccct ggggatcgga
    10441 atcgactaac agaacatcgg ccccggcgag ttgcagggcg cgggctagat gggttgcgat
    10501 ggtcgtcttg cctgacccgc ctttctggtt aagtacagcg ataaccttca tgcgttcccc
    10561 ttgcgtattt gtttatttac tcatcgcatc atatacgcag cgaccgcatg acgcaagctg
    10621 ttttactcaa atacacatca cctttttaga cggcggcgct cggtttcttc agcggccaag
    10681 ctggccggcc aggccgccag cttggcatca gacaaaccgg ccaggatttc atgcagccgc
    10741 acggttgaga cgtgcgcggg cggctcgaac acgtacccgg ccgcgatcat ctccgcctcg
    10801 atctcttcgg taatgaaaaa cggttcgtcc tggccgtcct ggtgcggttt catgcttgtt
    10861 cctcttggcg ttcattctcg gcggccgcca gggcgtcggc ctcggtcaat gcgtcctcac
    10921 ggaaggcacc gcgccgcctg gcctcggtgg gcgtcacttc ctcgctgcgc tcaagtgcgc
    10981 ggtacagggt cgagcgatgc acgccaagca gtgcagccgc ctctttcacg gtgcggcctt
    11041 cctggtcgat cagctcgcgg gcgtgcgcga tctgtgccgg ggtgagggta gggcgggggc
    11101 caaacttcac gcctcgggcc ttggcggcct cgcgcccgct ccgggtgcgg tcgatgatta
    11161 gggaacgctc gaactcggca atgccggcga acacggtcaa caccatgcgg ccggccggcg
    11221 tggtggtgtc ggcccacggc tctgccaggc tacgcaggcc cgcgccggcc tcctggatgc
    11281 gctcggcaat gtccagtagg tcgcgggtgc tgcgggccag gcggtctagc ctggtcactg
    11341 tcacaacgtc gccagggcgt aggtggtcaa gcatcctggc cagctccggg cggtcgcgcc
    11401 tggtgccggt gatcttctcg gaaaacagct tggtgcagcc ggccgcgtgc agttcggccc
    11461 gttggttggt caagtcctgg tcgtcggtgc tgacgcgggc atagcccagc aggccagcgg
    11521 cggcgctctt gttcatggcg taatgtctcc ggttctagtc gcaagtattc tactttatgc
    11581 gactaaaaca cgcgacaaga aaacgccagg aaaagggcag ggcggcagcc tgtcgcgtaa
    11641 cttaggactt gtgcgacatg tcgttttcag aagacggctg cactgaacgt cagaagccga
    11701 ctgcactata gcagcggagg ggttggatca aagtactttg atcccgaggg gaaccctgtg
    11761 gttggcatgc acatacaaat ggacgaacgg ataaaccttt tcacgccctt ttaaatatcc
    11821 gttattctaa taaacgctct tttctcttag gtttacccgc caatatatcc tgtcaaacac
    11881 tgatagttta aactgaaggc gggaaacgac aatctgatcc aagctcaagc tgctctagca
    11941 ttcgccattc aggctgcgca actgttggga agggcgatcg gtgcgggcct cttcgctatt
    12001 acgccagctg gcgaaagggg gatgtgctgc aaggcgatta agttgggtaa cgccagggtt
    12061 ttcccagtca cgacgttgta aaacgacggc cagtgcc
//
