LOCUS       40924_37408        9742 bp DNA     circular SYN 18-DEC-2018
DEFINITION  YAC construction vector pRML1, complete sequence.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 9742)
  AUTHORS   Knight TF Jr.
  TITLE     Direct Submission
  JOURNAL   Submitted (03-OCT-2006) CSAIL, MIT, 32 Vassar St, Cambridge, MA 
            02139, USA
REFERENCE   2  (bases 1 to 9742)
  TITLE     Direct Submission
REFERENCE   3  (bases 1 to 9742)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Submitted 
            (03-OCT-2006) CSAIL, MIT, 32 Vassar St, Cambridge, MA 02139, USA"
COMMENT     SGRef: number: 2; type: "Journal Article"
FEATURES             Location/Qualifiers
     source          1..9742
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        complement(52..70)
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     misc_feature    complement(664..956)
                     /label=CEN4
                     /note="S. cerevisiae CEN4 centromere"
     promoter        complement(973..1414)
                     /label=GAL1 promoter
                     /note="inducible promoter, regulated by Gal4"
     rep_origin      complement(1548..2385)
                     /direction=LEFT
                     /label=ARS1
                     /note="S. cerevisiae autonomously replicating sequence 
                     ARS1/ARS416"
     promoter        complement(2899..3180)
                     /label=TRP1 promoter
     promoter        3922..3984
                     /label=Mini-TK promoter
                     /note="minimal herpes simplex virus (HSV) thymidine kinase 
                     promoter"
     5'UTR           4747..4786
                     /label=HSV TK 5'-UTR
                     /note="5' untranslated region from the herpes simplex virus
                     thymidine kinase gene"
     CDS             4804..5931
                     /label=HSV TK
                     /note="herpes simplex virus thymidine kinase"
     polyA_signal    5976..6024
                     /label=HSV TK poly(A) signal
                     /note="herpes simplex virus thymidine kinase
                     polyadenylation signal (Cole and Stacy, 1985)"
     promoter        7061..7165
                     /label=AmpR promoter
     CDS             7166..8023
                     /label=AmpR
                     /note="beta-lactamase"
     rep_origin      8197..8785
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     misc_feature    9097..9723
                     /note="telomere region from pYAC4 of GenBank Accession
                     Number U01086"
ORIGIN
        1 atcgatttcg aagctagctc gcgagtcgac cgcggccgca gatctgaatt ccctttagtg
       61 agggttaatt tacgtagcga tgataagctg tcaaacatga gctgggccat tctcatgaag
      121 aatatcttga atttattgtc atattactag ctagttggtg tggaagtcct aatatcggtg
      181 atcaatatag tggttgacat gctggctagt caacattgag ccttttgatc atgcaaatat
      241 attacggtat tttacaatca aatatcaaac ttaactattg actttataac ttatttaggt
      301 ggtaacattc ttataaaaaa gaaaaaaatt actgcaaaac agtactagct tttaacttgt
      361 atcctaggtt atctatgctg tctcaccata gagaatatta cctatttcag aatgtatgtc
      421 catgattcgc cgggtaaata catataatac acaaatctgg cttaataaag tctataatat
      481 atctcataaa gaagtgctaa attggctagt gctatatatt tttaagaaaa tttcttttga
      541 ctaagtccat atcgactttg taaaagttca cattagcata catatattac acgagccaga
      601 aatagtaact tttgcctaaa tcacaaattg caaaatttaa ttgcttgcaa aaggtcacat
      661 gcttataatc aactttttta aaaatttaaa atactttttt attttttatt tttaaacata
      721 aatgaaataa tttatttatt gtttatgatt accgaaacat aaaacctgct caagaaaaag
      781 aaactgtttt gtccttggaa aaaaagcact acctaggagc ggccaaaatg ccgaggcttt
      841 catagcttaa actctttaca gaaaataggc attatagatc agttcgagtt ttcttattct
      901 tccttccggt tttatcgtca cagttttaca gtaaataagt atcacctctt agagttcgac
      961 ccggggtttt ttctccttga cgttaaagta tagaggtata ttaacaattt tttgttgata
     1021 cttttattac atttgaataa gaagtaatac aaaccgaaaa tgttgaaagt attagttaaa
     1081 gtggttatgc agtttttgca tttatatatc tgttaataga tcaaaaatca tcgcttcgct
     1141 gattaattac cccagaaata aggctaaaaa actaatcgca ttatcatcct atggttgtta
     1201 atttgattcg ttcatttgaa ggtttgtggg gccaggttac tgccaatttt tcctcttcat
     1261 aaccataaaa gctagtattg tagaatcttt attgttcgga gcagtgcggc gcgaggcaca
     1321 tctgcgtttc aggaacgcga ccggtgaaga cgaggacgca cggaggagag tcttccttcg
     1381 gagggctgtc acccgctcgg cggcttctaa tccgtactat gagataagca agtatcatct
     1441 catttcatta cctgaagtcg agtaaacaga aaatccaatt gttgatgaac ctcaatgact
     1501 tagaactatc tatcggcaga tcatataaag aggatttaga attaattcca catgttaaaa
     1561 tagtgaagga gcatgttcgg cacacagtgg accgaacgtg gggtaagtgc actagggtcc
     1621 ggttaaacgg atctcgcatt gatgaggcaa cgctaattat caacatatag attgttatct
     1681 atctgcatga acacgaaatc tttacttgac gacttgaggc tgatggtgtt tatgcaaaga
     1741 aaccactgtg tttaatatgt gtcactgttt gatattactg tcagcgtaga agataatagt
     1801 aaaagcggtt aataagtgta tttgagataa gtgtgataaa gtttttacag cgaaaagacg
     1861 ataaatacaa gaaaatgatt acgaggatac ggagagaggt atgtacatgt gtatttatat
     1921 actaagctgc cggcggttgt ttgcaagacc gagaaaaggc tagcaagaat cgggtcattg
     1981 tagcgtatgc gcctgtgaac attctcttca acaagtttga ttccattgcg gtgaaatggt
     2041 aaaagtcaac cccctgcgat gtatattttc ctgtacaatc aatcaaaaag ccaaatgatt
     2101 tagcattatc tttacatctt gttattttac agattttatg tttagatctt ttatgcttgc
     2161 ttttcaaaag gcctgcaggc aagtgcacaa acaatactta aataaatact actcagtaat
     2221 aacctatttc ttagcatttt tgacgaaatt tgctattttg ttagagtctt ttacaccatt
     2281 tgtctccaca cctccgctta catcaacacc aataacgcca tttaatctaa gcgcatcacc
     2341 aacattttct ggcgtcagtc caccagctaa cataaaatgt aagctttcgg ggctctcttg
     2401 ccttccaacc cagtcagaaa tcgagttcca atccaaaagt tcacctgtcc cacctgcttc
     2461 tgaatcaaac aagggaataa acgaatgagg tttctgtgaa gctgcactga gtagtatgtt
     2521 gcagtctttt ggaaatacga gtcttttaat aactggcaaa ccgaggaact cttggtattc
     2581 ttgccacgac tcatctccat gcagttggac gatatcaatg ccgtaatcat tgaccagagc
     2641 caaaacatcc tccttaggtt gattacgaaa cacgccaacc aagtatttcg gagtgcctga
     2701 actattttta tatgctttta caagacttga aattttcctt gcaataaccg ggtcaattgt
     2761 tctctttcta ttgggcacac atataatacc cagcaagtca gcatcggaat ctagagcaca
     2821 ttctgcggcc tctgtgctct gcaagccgca aactttcacc aatggaccag aactacctgt
     2881 gaaattaata acagacatac tccaagctgc ctttgtgtgc ttaatcacgt atactcacgt
     2941 gctcaatagt caccaatgcc ctccctcttg gccctctcct tttctttttt cgaccgaatt
     3001 aattcttaat cggcaaaaaa agaaaagctc cggatcaaga ttgtacgtaa ggtgacaagc
     3061 tatttttcaa taaagaatat cttccactac tgccatctgg cgtcataact gcaaagtaca
     3121 catatattac gatgctgttc tattaaatgc ttcctatatt atatatatag taatgtcgtt
     3181 aaccgttgat ttttactgat tggtctctgc cgtgcgggtg agcggcgcgt ccagacgggc
     3241 gggtgcacgt ttctttttct gaccatttcc ctgtcagctc ttttagatcg gggctgccgt
     3301 ttgaaaagtt ttatcatcgc cagacatgcc ataaggaata tctccaccgc ctctaactgt
     3361 ccctaactcg gtctctttct tataactacc attactcagt gttgagagat tggtagaaat
     3421 atacgcgtat acgttcattc aagacttatt ttcagccagt aatattccct actcctcttc
     3481 cgttttcaga atagagatgg accagccaag gacccattca ggacccacga cggccagcaa
     3541 tcctgccccc agctccacca actcgtcatc tgctcccagc gcgactaatt caaagcagga
     3601 aagatccaca ggacgggtgt ggtcgccatg atcgcgtagt cgatagtggc tccaagtagc
     3661 gaagcgagca ggactgggcg gcggccaaag cggtcggaca gtgctccgag aacgggtgcg
     3721 catagaaatt gcatcaacgc atatagcgct agcagcacgc catagtgact ggcgatgctg
     3781 tcggaatgga cgataattct tgaagacgaa agggcctcgt gatacgccta tttttatagg
     3841 ttaatgtcat gataataatg gtttcttaga atttaattcg aacacgcaga tgcagtcggg
     3901 gcggcgcggt cccaggtcca cttcgcatat taaggtgacg cgtgtggcct cgaataccga
     3961 gcgaccctgc agcgacccgc ttaacagcgt caacagcgtg ccgcagatct ctcttgcgag
     4021 atgatcccgc attttcttga aagctttgca gaggctagca gaattaccct ccacgttgat
     4081 tgtctgcgag gcaagaatga tcatcaccgt agtgagagtg cgttcaaggc tcttgcggtt
     4141 gccataagag aagccacctc gcccaatggt accaacgatg ttccctccac caaaggtgtt
     4201 cttatgtagt gacaccgatt atttaaagct gcagcatacg atatatatac atgtgtatat
     4261 atgtatacct atgaatgtca gtaagtatgt atacgaacag tatgatactg aagatgacaa
     4321 ggtaatgcat cattctatac gtgtcattct gaacgaggcg cgctttcctt ttttcttttt
     4381 gctttttctt tttttttctc ttgaactcga gaaaaaaaat ataaaagaga tggaggaacg
     4441 ggaaaaagtt agttgtggtg ataggtggca agtggtattc cgtaagaaca acaagaaaag
     4501 catttcatat tatggctgaa ctgagcgaac aagtgcaaaa tttaagcatc aacgacaaca
     4561 acgagaatgg ttatgttcct cctcacttaa gaggaaaacc aagaagtgcc agaaataaca
     4621 gtagcaacta caataacaac aacggcggct acaacggtgg ccgtggcggt ggcagcttct
     4681 ttagcaacaa ccgtcgtggt ggttacggca acggtggttt cttcggtgga aacaacggtg
     4741 gcagcagatc ttggtggcgt gaaactcccg cacctcttcg gccagcgcct tgtagaagcg
     4801 cgtatggctt cgtacccctg ccatcaacac gcgtctgcgt tcgaccaggc tgcgcgttct
     4861 cgcggccata acaaccgacg tacggcgttg cgccctcgcc ggcaacaaaa agccacggaa
     4921 gtccgcctgg agcagaaaat gcccacgcta ctgcgggttt atatagacgg tccccacggg
     4981 atggggaaaa ccaccaccac gcaactgctg gtggccctgg gttcgcgcga cgatatcgtc
     5041 tacgtacccg agccgatgac ttactggcgg gtgttggggg cttccgagac aatcgcgaac
     5101 atctacacca cacaacaccg cctcgaccag ggtgagatat cggccgggga cgcggcggtg
     5161 gtaatgacaa gcgcccagat aacaatgggc atgccttatg ccgtgaccga cgccgttctg
     5221 gctcctcata tcggggggga ggctgggagc tcacatgccc cgcccccggc cctcaccctc
     5281 atcttcgacc gccatcccat cgccgccctc ctgtgctacc cggccgcgcg ataccttatg
     5341 ggcagcatga ccccccaggc cgtgctggcg ttcgtggccc tcatcccgcc gaccttgccc
     5401 ggcacaaaca tcgtgttggg ggcccttccg gaggacagac acatcgaccg cctggccaaa
     5461 cgccagcgcc ccggcgagcg gcttgacctg gctatgctgg ccgcgattcg ccgcgtttat
     5521 gggctgcttg ccaatacggt gcggtatctg cagggcggcg ggtcgtggcg ggaggattgg
     5581 ggacagcttt cgggggcggc cgtgccgccc cagggtgccg agccccagag caacgcgggc
     5641 ccacgacccc atatcgggga cacgttattt accctgtttc gggcccccga gttgctggcc
     5701 cccaacggcg acctgtataa cgtgtttgcc tgggctttgg acgtcttggc caaacgcctc
     5761 cgtcccatgc acgtctttat cctggattac gaccaatcgc ccgccggctg ccgggacgcc
     5821 ctgctgcaac ttacctccgg gatggtccag acccacgtca ccaccccagg ctccataccg
     5881 acgatctgcg acctggcgcg cacgtttgcc cgggagatgg gggaggctaa ctgaaacacg
     5941 gaaggagaca ataccggaag gaacccgcgc tatgacggca ataaaaagac agaataaaac
     6001 gcacgggtgt tgggtcgttt gttcataaac gcggggttcg gtcccagggc tggcactctg
     6061 tcgatacccc accgagaccc cattgggacc aatacgcccg cgtttcttcc ttttccccac
     6121 cccaaccccc aagttcgggt gaaggcccag ggctcgcagc caacgtcggg gcggcaagcc
     6181 ctgccatagc cacgggcccc gtgggttagg gacggggtcc cccatgggga atggtttatg
     6241 gttcgtgggg gttattattt tgggcgttgc gtggggtcag gtccacgact ggactgagca
     6301 gacagaccca tggtttttgg atggcctggg catggaccgc atgtactggc gcgacacgaa
     6361 caccgggcgt ctgtggctgc caaacacccc cgacccccaa aaaccaccgc gcggatttct
     6421 ggcgccgccg gacgaactaa acctgactac ggcatctctg ccccttcttc gctggtacga
     6481 ggagcgcttt tgttttgtat tggtcaccac ggccgagttt ccgcgggacc ccggccagct
     6541 gctttacatc ccgaagacct acctgctcgg ccggcccccg aacgcgagcc tgcccgcccc
     6601 caccacggtc gagccgaccg cccagcctcc cccctcggtc gcccccctta agggtctctt
     6661 gcacaatcca gccgcctccg tgttgctgcg ttcccgggcc tgggtaacgt tttcggccgt
     6721 ccctgacccc gaggccctga cgttcccgcg gggagacaac gtggcgacgg cgagccaccc
     6781 gagcgggccg cgtgatacac cgcccccccg accgccggtt ggggcccggc ggcacccgac
     6841 gacggagctg gacatcacgc acctgcacaa cgcgtccacg acctggttgg ccacccgggg
     6901 cctgttgaga tccccaggta ggtacgtgta tttctccccg tcggcctcga cgtggcccgt
     6961 gggcatctgg acgacggggg agctggtgct cgggtgcgat gccgcgctgg tgcgcgcgcg
     7021 ctacgggcgg gaattaggtg gcacttttcg gggaaatgtg cgcggaaccc ctatttgttt
     7081 atttttctaa atacattcaa atatgtatcc gctcatgaga caataaccct gataaatgct
     7141 tcaataatat tgaaaaagga agagtatgag tattcaacat ttccgtgtcg cccttattcc
     7201 cttttttgcg gcattttgcc ttcctgtttt tgctcaccca gaaacgctgg tgaaagtaaa
     7261 agatgctgaa gatcagttgg gtgcacgagt gggttacatc gaactggatc tcaacagcgg
     7321 taagatcctt gagagttttc gccccgaaga acgttttcca atgatgagca cttttaaagt
     7381 tctgctatgt ggcgcggtat tatcccgtgt tgacgccggg caagagcaac tcggtcgccg
     7441 catacactat tctcagaatg acttggttga gtactcacca gtcacagaaa agcatcttac
     7501 ggatggcatg acagtaagag aattatgcag tgctgccata accatgagtg ataacactgc
     7561 ggccaactta cttctgacaa cgatcggagg accgaaggag ctaaccgctt ttttgcacaa
     7621 catgggggat catgtaactc gccttgatcg ttgggaaccg gagctgaatg aagccatacc
     7681 aaacgacgag cgtgacacca cgatgcctgt agcaatggca acaacgttgc gcaaactatt
     7741 aactggcgaa ctacttactc tagcttcccg gcaacaatta atagactgga tggaggcgga
     7801 taaagttgca ggaccacttc tgcgctcggc ccttccggct ggctggttta ttgctgataa
     7861 atctggagcc ggtgagcgtg ggtctcgcgg tatcattgca gcactggggc cagatggtaa
     7921 gccctcccgt atcgtagtta tctacacgac ggggagtcag gcaactatgg atgaacgaaa
     7981 tagacagatc gctgagatag gtgcctcact gattaagcat tggtaactgt cagaccaagt
     8041 ttactcatat atactttaga ttgatttaaa acttcatttt taatttaaaa ggatctaggt
     8101 gaagatcctt tttgataatc tcatgaccaa aatcccttaa cgtgagtttt cgttccactg
     8161 agcgtcagac cccgtagaaa agatcaaagg atcttcttga gatccttttt ttctgcgcgt
     8221 aatctgctgc ttgcaaacaa aaaaaccacc gctaccagcg gtggtttgtt tgccggatca
     8281 agagctacca actctttttc cgaaggtaac tggcttcagc agagcgcaga taccaaatac
     8341 tgtccttcta gtgtagccgt agttaggcca ccacttcaag aactctgtag caccgcctac
     8401 atacctcgct ctgctaatcc tgttaccagt ggctgctgcc agtggcgata agtcgtgtct
     8461 taccgggttg gactcaagac gatagttacc ggataaggcg cagcggtcgg gctgaacggg
     8521 gggttcgtgc acacagccca gcttggagcg aacgacctac accgaactga gatacctaca
     8581 gcgtgagcta tgagaaagcg ccacgcttcc cgaagggaga aaggcggaca ggtatccggt
     8641 aagcggcagg gtcggaacag gagagcgcac gagggagctt ccagggggaa acgcctggta
     8701 tctttatagt cctgtcgggt ttcgccacct ctgacttgag cgtcgatttt tgtgatgctc
     8761 gtcagggggg cggagcctat ggaaaaacgc cagcaacgcg gcctttttac ggttcctggc
     8821 cttttgctgg ccttttgctc acatgttctt tcctgcgtta tcccctgatt ctgtggataa
     8881 ccgtattacc gcctttgagt gagctgatac cgctcgccgc agccgaacga ccgagcgcag
     8941 cgagtcagtg agcgaggaag cggaagagcg cctgatgcgg tattttctcc ttacgcatct
     9001 gtgcggtatt tcacaccgca tacggtaccc ggggatccat atgactagtg atcctctaga
     9061 gtcgatcgac ctgcaggcat gcctgcaggt cgaggcgata agcttcattt ttagataaaa
     9121 tttattaatc atcattaatt tcttgaaaaa cattttattt attgatcttt tataacaaaa
     9181 aacccttcta aaagtttatt tttgaatgaa aaacttataa aaatttatga aaactacaaa
     9241 aaataaaatt tttaattaaa ataattttga taagaacttc aatctttgac tagcttagtc
     9301 atttttgaga tttaattaat attttatgtt tattcatata taaactattc aaaatattat
     9361 agaatttaaa cattttaaca tcttaatcat tcataaataa ctaaaaatca aagtattaca
     9421 ttcaataaat aacttttact caatgtcaaa gaaattattg gggttggggt tggggttggg
     9481 gttggggttg gggttggggt tggggttggg gttggggttg gggttggggt tggggttggg
     9541 gttggggttg gggttggggt tggggttggg gttggggttg gggttggggt tggggttggg
     9601 gttggggttg gggttggggt tggggttggg gttggggttg gggttggggt tgggggttgg
     9661 ggttggggtt ggggttgggg ttggggttgg ggttggggtt ggggttgggg ttggggttgg
     9721 ggtctgcctc gcgcggaaat tc
//
