LOCUS       V003677                 9038 bp    DNA     circular SYN 18-DEC-2018
DEFINITION  Exported.
ACCESSION   V003677
VERSION     V003677
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
            .
REFERENCE   1  (bases 1 to 9038)
  AUTHORS   De Schamphelaire W, Olbrechts A, Meert J, Verhelst K, Roggeman
            Fonseca M, Vanhoucke M, Beyaert R.
  TITLE     BCCM/LMBP Plasmid collection
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 9038)
  AUTHORS   De Schamphelaire W.
  TITLE     Direct Submission
  JOURNAL   Submitted (02-FEB-2017) BCCM/LMBP, Universiteit Gent,
            Technologiepark 927, 9052, BELGIUM
REFERENCE   3  (bases 1 to 9038)
  TITLE     Direct Submission
REFERENCE   4  (bases 1 to 9038)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName:
            "Unpublished"
            SGRef: number: 2; type: "Journal Article"; journalName: "Submitted
            (02-FEB-2017) BCCM/LMBP, Universiteit Gent, Technologiepark 927,
            9052, BELGIUM"
            SGRef: number: 3; type: "Journal Article"
FEATURES             Location/Qualifiers
     source          1..9038
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     rep_origin      complement(63..763)
                     /direction=LEFT
                     /label="ARS2"
                     /note="ARS2"
     CDS             complement(1665..2474)
                     /gene="URA3"
                     /label="Orotidine 5'-phosphate decarboxylase"
                     /note="Orotidine 5'-phosphate decarboxylase from Candida
                     albicans (strain SC5314 / ATCC MYA-2876). Accession#:
                     P13649"
     promoter        complement(3202..3306)
                     /gene="bla"
                     /label="AmpR promoter"
     primer_bind     3578..3594
                     /label="M13 fwd"
                     /note="common sequencing primer, one of multiple similar
                     variants"
     promoter        3829..4179
                     /label="MET17 promoter"
                     /note="expression is repressed by methionine"
     CDS             4180..5511
                     /label="MET17"
                     /note="O-acetylserine and O-acetylhomoserine sulfhydrylase,
                     required for sulfur amino acid synthesis"
     misc_feature    5516..6097
                     /label="3' UTR of ScMET15"
                     /note="3' UTR of ScMET15"
     rep_origin      6103..6783
                     /label="ARS3"
                     /note="ARS3"
     primer_bind     complement(6817..6833)
                     /label="M13 rev"
                     /note="common sequencing primer, one of multiple similar
                     variants"
     protein_bind    complement(6841..6857)
                     /label="lac operator"
                     /note="The lac repressor binds to the lac operator to
                     inhibit transcription in E. coli. This inhibition can be
                     relieved by adding lactose or
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(6865..6895)
                     /label="lac promoter"
                     /note="promoter for the E. coli lac operon"
     protein_bind    complement(6910..6931)
                     /label="CAP binding site"
                     /note="CAP binding activates transcription in the presence
                     of cAMP."
     rep_origin      complement(7219..7807)
                     /direction=LEFT
                     /label="ori"
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
                     replication"
     CDS             complement(7981..8838)
                     /label="AmpR"
                     /note="beta-lactamase"
     promoter        complement(8839..8943)
                     /label="AmpR promoter"
ORIGIN
        1 tcgcgcgcga gcccatttat acccatataa atcagcatcc atgttggaat ttaatcgcgg
       61 ccgctacgaa tgttagagac aaaatgtagt ccaggaccgg ccagacgcag tgtgcgtgct
      121 ggttgtgcaa ttatactagc acatctaggt gttttaggaa gaaacgtcca ccaccaaaaa
      181 attaatttcc aaatattggc acttttatta caccagtggt gttacacagc cccccaaaca
      241 ctatcccggt tgttagattg aagtttgttt aacaaaaatt ggaagttcta ttttttactt
      301 tttgtacaaa atttggcaag aaaattggag taaaatattt ttatataaat ttcaaactaa
      361 aaatatccaa aacataaaat aatacacaaa atgagatgct ctatttagcc aaaccaacca
      421 ttacgggtgt gttgtttggt gttgtctgac catgggtata ccatttgtta gtgtatagct
      481 gcactgtttt atgtttttcg atttttccac ataaaaggag tactcctata ctaataacaa
      541 acactccact ataattggca accacaatta tgcctgggga aatattttcc actccgagtg
      601 tacatacatt tttggtgtgt tgttggtggt tgaaaattgt gtgggtcacg tgcatttaat
      661 tagggttagg gctacggttt gtgtgcagta ccacgttcaa attgaggctc tctggtataa
      721 cttgcactac ctcatcgcta ccccggattt ttttttggta tgatcctcta cgccggacgc
      781 atcgtggccg gcatcaccgg cgccacaggt gcggttgctg gcgcctatat cgccgacatc
      841 accgatgggg aagatcgggc tcgccacttc gggctcatga gcgcttgttt cggcgtgggt
      901 aaggtggcag gctcggtggc cgggggactg ttgggcgcca tctccttgca tgcaccattc
      961 cttgcggcgg cggtgctcaa cggcctcaac ctactactgg gctgcttcct aatgcaggag
     1021 tcgcataagg gagagcgtcg agcaagacgt ttcccgttga atatggctca taacacccct
     1081 tgtattactg tttatgtaag cagacagttt tattgttcat gatgatatat ttttatcttg
     1141 tgcaatgtaa catcagagat tttgagacac aacgtggctt tgttgaataa atcgaacttt
     1201 tgctgagttg aaggatcaga tcacgcatct tcccgacaac gcagaccgtt ccgtggcaaa
     1261 gcaaaagttc aaaatcacca actggtccac ctacaacaaa gctctcatca accgtggctc
     1321 cctcactttc tggctggatg atggggcgat tcaggcctgg tatgagtcag caacaccttc
     1381 ttcacgaggc agacctcagc gctattctga ccttgccatc acgactgtgc tggtcattaa
     1441 acgcgtattc aggctgaccc tgcgcgctgc gcagggcttt attgattcca tttttacact
     1501 gatgaatgtt ccgttgcgct gcccggatta cagctagaag gaccaccttt gattgtaaat
     1561 agtaataatt accaccctta tttaattatt tatttaactt atttatttat ttattctaca
     1621 tatatacaaa tctaataaag tgaaaatctc ccccttcaca tttataattg gccagtcttt
     1681 ttcaaataag cattccaacc agcatttcta taccttttac cttcaatatc tggatctctt
     1741 cctttaccaa acaatcctct accaacaatg ataatatcag ttccagtgct aacaacttca
     1801 tcaacagttc tatattgttg tcctaatcca tcacctttat catctaatcc aactccaggt
     1861 gtcataataa gccaatcaaa tccttcttct tggccaccca tatcacgttg ggcaataaat
     1921 ccaataacaa attccttatc ggatttagca atttcaacag ttttttgaga atattctcca
     1981 tatgctaatg atcccactga tgataattca gctaacatca ataaccctct tggctcttgg
     2041 ttggtggtgg tttctttagc tccctgtttt aatccttcaa ccactccatt cccagtgaca
     2101 ccatgagcat tggtaatatc tgcccaacta ctaattttat aaactccacc aatatattgt
     2161 ttctttacgg tattaccaat atcagcaaat tttctatctt caaaaatcat aaattgatgt
     2221 ttacgtgaaa gttctaataa tggttcaata gtggattcat aggaaaaatc attgattata
     2281 tcaatatgag tcttgattaa gcatacataa ggacctaatt tatcaattaa ttcaaggaat
     2341 tccttagtgg tatcaacgtc aattgatgca cataaattgg ttttcttcag ttccattaat
     2401 cgaaataatc gctgtgctac tggtgaggca tgagtttctg ctctctcact ataggtctta
     2461 gtgttgactg tcatatcttt tgtttgctat ttctatttct atttctttct tcaactgtaa
     2521 aaggggcggt atataaacca gtgtgtatgg ggttgttgct caggaattga tcaatgttta
     2581 aaagaatggt tgaaatcatc attgaagaac aaaaaaaaag attttttctt tcattatgtt
     2641 atctgtataa tatgcaggaa ataagattgc aaaaaaatag ccgttttttt ggatactatc
     2701 aaacaagagg aatgactaat acagaattag tgttacgaat caatggcact acagcaactt
     2761 tcaacaaaat aatctacgtt caaaatataa tatatactat taaataaaat caaataataa
     2821 aataatttta aagaaagtag taccagctct tttttttgtt tccgtttata ccatccaaat
     2881 caattcctat tagtactcaa ccaagtcatt ctgagaatag tgtatgcggc gaccgagttg
     2941 ctcttgcccg gcgtcaacac gggataatac cgcgccacat agcagaactt taaaagtgct
     3001 catcattgga aaacgttctt cggggcgaaa actctcaagg atcttaccgc tgttgagatc
     3061 cagttcgatg taacccactc gtgcacccaa ctgatcttca gcatctttta ctttcaccag
     3121 cgtttctggg tgagcaaaaa caggaaggca aaatgccgca aaaaagggaa taagggcgac
     3181 acggaaatgt tgaatactca tactcttcct ttttcaatat tattgaagca tttatcaggg
     3241 ttattgtctc atgagcggat acatatttga atgtatttag aaaaataaac aaataggggt
     3301 tccgcgcaca tttccccgaa aagtgccacc tgacgtctaa gaaaccatta ttatcatgac
     3361 attaacctat aaaaataggc gtatcacgag gccctttcgt cttcaagaat tctcatgttt
     3421 gacagcttat catcgcgcca ttcgccattc aggctgcgca actgttggga agggcgatcg
     3481 gtgcgggcct cttcgctatt acgccagctg gcgaaagggg gatgtgctgc aaggcgatta
     3541 agttgggtaa cgccagggtt ttcccagtca cgacgttgta aaacgacggc cagtgaattc
     3601 ccctagttag tagatgatag ttgattttta ttccaacact aagaaataat ttcgccattt
     3661 cttgaatgta tttaaagata tttaatgcta taatagacat ttaaatccaa ttcttccaac
     3721 atacaatggg agtttggccg agtggtttaa ggcgtcagat ttaggtggat ttaacctcta
     3781 aaatctctga tatcttcgga tgcaagggtt cgaatccctt agctctcatt attttttgct
     3841 ttttctcttg aggtcacatg atcgcaaaat ggcaaatggc acgtgaagct gtcgatattg
     3901 gggaactgtg gtggttggca aatgactaat taagttagtc aaggcgccat cctcatgaaa
     3961 actgtgtaac ataataaccg aagtgtcgaa aaggtggcac cttgtccaat tgaacacgct
     4021 cgatgaaaaa aataagatat atataaggtt aagtaaagcg tctgttagaa aggaagtttt
     4081 tcctttttct tgctctcttg tcttttcatc tactatttcc ttcgtgtaat acagggtcgt
     4141 cagatacata gatacaattc tattaccccc atccatacaa tgccatctca tttcgatact
     4201 gttcaactac acgccggcca agagaaccct ggtgacaatg ctcacagatc cagagctgta
     4261 ccaatttacg ccaccacttc ttatgttttc gaaaactcta agcatggttc gcaattgttt
     4321 ggtctagaag ttccaggtta cgtctattcc cgtttccaaa acccaaccag taatgttttg
     4381 gaagaaagaa ttgctgcttt agaaggtggt gctgctgctt tggctgtttc ctccggtcaa
     4441 gccgctcaaa cccttgccat ccaaggtttg gcacacactg gtgacaacat cgtttccact
     4501 tcttacttat acggtggtac ttataaccag ttcaaaatct cgttcaaaag atttggtatc
     4561 gaggctagat ttgttgaagg tgacaatcca gaagaattcg aaaaggtctt tgatgaaaga
     4621 accaaggctg tttatttgga aaccattggt aatccaaagt acaatgttcc ggattttgaa
     4681 aaaattgttg caattgctca caaacacggt attccagttg tcgttgacaa cacatttggt
     4741 gccggtggtt acttctgtca gccaattaaa tacggtgctg atattgtaac acattctgct
     4801 accaaatgga ttggtggtca tggtactact atcggtggta ttattgttga ctctggtaag
     4861 ttcccatgga aggactaccc agaaaagttc cctcaattct ctcaacctgc cgaaggatat
     4921 cacggtacta tctacaatga agcctacggt aacttggcat acatcgttca tgttagaact
     4981 gaactattaa gagatttggg tccattgatg aacccatttg cctctttctt gctactacaa
     5041 ggtgttgaaa cattatcttt gagagctgaa agacacggtg aaaatgcatt gaagttagcc
     5101 aaatggttag aacaatcccc atacgtatct tgggtttcat accctggttt agcatctcat
     5161 tctcatcatg aaaatgctaa gaagtatcta tctaacggtt tcggtggtgt cttatctttc
     5221 ggtgtaaaag acttaccaaa tgccgacaag gaaactgacc cattcaaact ttctggtgct
     5281 caagttgttg acaatttaaa gcttgcctct aacttggcca atgttggtga tgccaagacc
     5341 ttagtcattg ctccatactt cactacccac aaacaattaa atgacaaaga aaagttggca
     5401 tctggtgtta ccaaggactt aattcgtgtc tctgttggta tcgaatttat tgatgacatt
     5461 attgcagact tccagcaatc ttttgaaact gttttcgctg gccaaaaacc atgagtgtgc
     5521 gtaatgagtt gtaaaattat gtataaacct actttctctc acaagtacta tacttttata
     5581 aaacgaactt tattgaaatg aatatccttt ttttcccttg ttacatgtcg tgactcgtac
     5641 tttgaaccta aattgttcta acatcaaaga acagtgttaa ttcgcagtcg agaagaaaaa
     5701 tatggtgaac aagactcatc tacttcatga gactacttta cgcctcctat aaagctgtca
     5761 cactggataa atttattgta ggaccaagtt acaaaagagg atgatggagg tttctttaca
     5821 ataaagaagc acatgtgtgt taacgttttt agtatttgct tgttatgtaa atcaggaaaa
     5881 cttcgcggga tttggttgga tgctactttc catacaataa atattataga tctaaaaagc
     5941 caaattacaa gtaaagatta gtaaagctgt tggaattcca tcgttgataa aaatgttagt
     6001 tattaaatat aaaagtcaga ataggtgaac ttggatttaa ttgttggcat ttcgttgctg
     6061 ctagaggcca taatattaga tagccaggac atactagggg gatccagact tgttaaatct
     6121 gatgttttga cgattatcat tgaatgtaag atcaataaac tattctcttt ctttttattt
     6181 caaaaccttg cagtaccatg gaattatatc aagccatgtt ttaaattgat cagtagtcta
     6241 atcgggcttc aagggaaaag gtggtttact cgcttaacga tacttttgac atttctacta
     6301 cttagattgt aagagagcat tggtagcatc aaaaacacac aaaatataat cattttccca
     6361 attgggaaca attcaaaata aaaacaactt tgatgcgaag aacttcaata ttttagctaa
     6421 ttatatcaaa attggaatgg agtctctact actacatgct tttctcaatg aaattacgtt
     6481 aaaacttttc tcgtcttctt ccattttttt ttatattctt tactttttct ttaatttttt
     6541 tttgtcattc cttatttaaa tttccagaat accctttaaa ctacataata tcatgaagaa
     6601 tccatttgac agtggcagtg acgatgaaga tccatttctt agtaatccac aatctgcacc
     6661 atcaatgccc tacgcagcat atactagtga cagaacatcg ccccgcaaga cataccaacc
     6721 attgaatttt gacagtgagg acgaagatgc taaagaaagc gaatttatgg ctttcccact
     6781 gtcgacctgc aggcatgcaa gcttggcgta atcatggtca tagctgtttc ctgtgtgaaa
     6841 ttgttatccg ctcacaattc cacacaacat acgagccgga agcataaagt gtaaagcctg
     6901 gggtgcctaa tgagtgagct aactcacatt aattgcgttg cgctcactgc ccgctttcca
     6961 gtcgggaaac ctgtcgtgcc agctgcatta atgaatcggc caacgcgcgg ggagaggcgg
     7021 tttgcgtatt gggcgctctt ccgcttcctc gctcactgac tcgctgcgct cggtcgttcg
     7081 gctgcggcga gcggtatcag ctcactcaaa ggcggtaata cggttatcca cagaatcagg
     7141 ggataacgca ggaaagaaca tgtgagcaaa aggccagcaa aaggccagga accgtaaaaa
     7201 ggccgcgttg ctggcgtttt tccataggct ccgcccccct gacgagcatc acaaaaatcg
     7261 acgctcaagt cagaggtggc gaaacccgac aggactataa agataccagg cgtttccccc
     7321 tggaagctcc ctcgtgcgct ctcctgttcc gaccctgccg cttaccggat acctgtccgc
     7381 ctttctccct tcgggaagcg tggcgctttc tcatagctca cgctgtaggt atctcagttc
     7441 ggtgtaggtc gttcgctcca agctgggctg tgtgcacgaa ccccccgttc agcccgaccg
     7501 ctgcgcctta tccggtaact atcgtcttga gtccaacccg gtaagacacg acttatcgcc
     7561 actggcagca gccactggta acaggattag cagagcgagg tatgtaggcg gtgctacaga
     7621 gttcttgaag tggtggccta actacggcta cactagaagg acagtatttg gtatctgcgc
     7681 tctgctgaag ccagttacct tcggaaaaag agttggtagc tcttgatccg gcaaacaaac
     7741 caccgctggt agcggtggtt tttttgtttg caagcagcag attacgcgca gaaaaaaagg
     7801 atctcaagaa gatcctttga tcttttctac ggggtctgac gctcagtgga acgaaaactc
     7861 acgttaaggg attttggtca tgagattatc aaaaaggatc ttcacctaga tccttttaaa
     7921 ttaaaaatga agttttaaat caatctaaag tatatatgag taaacttggt ctgacagtta
     7981 ccaatgctta atcagtgagg cacctatctc agcgatctgt ctatttcgtt catccatagt
     8041 tgcctgactc cccgtcgtgt agataactac gatacgggag ggcttaccat ctggccccag
     8101 tgctgcaatg ataccgcgag acccacgctc accggctcca gatttatcag caataaacca
     8161 gccagccgga agggccgagc gcagaagtgg tcctgcaact ttatccgcct ccatccagtc
     8221 tattaattgt tgccgggaag ctagagtaag tagttcgcca gttaatagtt tgcgcaacgt
     8281 tgttgccatt gctacaggca tcgtggtgtc acgctcgtcg tttggtatgg cttcattcag
     8341 ctccggttcc caacgatcaa ggcgagttac atgatccccc atgttgtgca aaaaagcggt
     8401 tagctccttc ggtcctccga tcgttgtcag aagtaagttg gccgcagtgt tatcactcat
     8461 ggttatggca gcactgcata attctcttac tgtcatgcca tccgtaagat gcttttctgt
     8521 gactggtgag tactcaacca agtcattctg agaatagtgt atgcggcgac cgagttgctc
     8581 ttgcccggcg tcaatacggg ataataccgc gccacatagc agaactttaa aagtgctcat
     8641 cattggaaaa cgttcttcgg ggcgaaaact ctcaaggatc ttaccgctgt tgagatccag
     8701 ttcgatgtaa cccactcgtg cacccaactg atcttcagca tcttttactt tcaccagcgt
     8761 ttctgggtga gcaaaaacag gaaggcaaaa tgccgcaaaa aagggaataa gggcgacacg
     8821 gaaatgttga atactcatac tcttcctttt tcaatattat tgaagcattt atcagggtta
     8881 ttgtctcatg agcggataca tatttgaatg tatttagaaa aataaacaaa taggggttcc
     8941 gcgcacattt ccccgaaaag tgccacctga cgtctaagaa accattatta tcatgacatt
     9001 aacctataaa aataggcgta tcacgaggcc ctttcgtc
//
