LOCUS       V003678                 6543 bp    DNA     circular SYN 18-DEC-2018
DEFINITION  Exported.
ACCESSION   V003678
VERSION     V003678
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
            .
REFERENCE   1  (bases 1 to 6543)
  AUTHORS   De Schamphelaire W, Olbrechts A, Meert J, Verhelst K, Roggeman
            Fonseca M, Vanhoucke M, Beyaert R.
  TITLE     BCCM/LMBP Plasmid collection
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 6543)
  AUTHORS   De Schamphelaire W.
  TITLE     Direct Submission
  JOURNAL   Submitted (02-FEB-2017) BCCM/LMBP, Universiteit Gent,
            Technologiepark 927, 9052, BELGIUM
REFERENCE   3  (bases 1 to 6543)
  TITLE     Direct Submission
REFERENCE   4  (bases 1 to 6543)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName:
            "Unpublished"
            SGRef: number: 2; type: "Journal Article"; journalName: "Submitted
            (02-FEB-2017) BCCM/LMBP, Universiteit Gent, Technologiepark 927,
            9052, BELGIUM"
            SGRef: number: 3; type: "Journal Article"
FEATURES             Location/Qualifiers
     source          1..6543
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     rep_origin      complement(63..763)
                     /direction=LEFT
                     /label="ARS2"
                     /note="ARS2"
     CDS             complement(1665..2474)
                     /gene="URA3"
                     /label="Orotidine 5'-phosphate decarboxylase"
                     /note="Orotidine 5'-phosphate decarboxylase from Candida
                     albicans (strain SC5314 / ATCC MYA-2876). Accession#:
                     P13649"
     promoter        complement(3202..3306)
                     /gene="bla"
                     /label="AmpR promoter"
     primer_bind     3578..3594
                     /label="M13 fwd"
                     /note="common sequencing primer, one of multiple similar
                     variants"
     rep_origin      3608..4288
                     /label="ARS3"
                     /note="ARS3"
     primer_bind     complement(4322..4338)
                     /label="M13 rev"
                     /note="common sequencing primer, one of multiple similar
                     variants"
     protein_bind    complement(4346..4362)
                     /label="lac operator"
                     /note="The lac repressor binds to the lac operator to
                     inhibit transcription in E. coli. This inhibition can be
                     relieved by adding lactose or
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(4370..4400)
                     /label="lac promoter"
                     /note="promoter for the E. coli lac operon"
     protein_bind    complement(4415..4436)
                     /label="CAP binding site"
                     /note="CAP binding activates transcription in the presence
                     of cAMP."
     rep_origin      complement(4724..5312)
                     /direction=LEFT
                     /label="ori"
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
                     replication"
     CDS             complement(5486..6343)
                     /label="AmpR"
                     /note="beta-lactamase"
     promoter        complement(6344..6448)
                     /label="AmpR promoter"
ORIGIN
        1 tcgcgcgcga gcccatttat acccatataa atcagcatcc atgttggaat ttaatcgcgg
       61 ccgctacgaa tgttagagac aaaatgtagt ccaggaccgg ccagacgcag tgtgcgtgct
      121 ggttgtgcaa ttatactagc acatctaggt gttttaggaa gaaacgtcca ccaccaaaaa
      181 attaatttcc aaatattggc acttttatta caccagtggt gttacacagc cccccaaaca
      241 ctatcccggt tgttagattg aagtttgttt aacaaaaatt ggaagttcta ttttttactt
      301 tttgtacaaa atttggcaag aaaattggag taaaatattt ttatataaat ttcaaactaa
      361 aaatatccaa aacataaaat aatacacaaa atgagatgct ctatttagcc aaaccaacca
      421 ttacgggtgt gttgtttggt gttgtctgac catgggtata ccatttgtta gtgtatagct
      481 gcactgtttt atgtttttcg atttttccac ataaaaggag tactcctata ctaataacaa
      541 acactccact ataattggca accacaatta tgcctgggga aatattttcc actccgagtg
      601 tacatacatt tttggtgtgt tgttggtggt tgaaaattgt gtgggtcacg tgcatttaat
      661 tagggttagg gctacggttt gtgtgcagta ccacgttcaa attgaggctc tctggtataa
      721 cttgcactac ctcatcgcta ccccggattt ttttttggta tgatcctcta cgccggacgc
      781 atcgtggccg gcatcaccgg cgccacaggt gcggttgctg gcgcctatat cgccgacatc
      841 accgatgggg aagatcgggc tcgccacttc gggctcatga gcgcttgttt cggcgtgggt
      901 aaggtggcag gctcggtggc cgggggactg ttgggcgcca tctccttgca tgcaccattc
      961 cttgcggcgg cggtgctcaa cggcctcaac ctactactgg gctgcttcct aatgcaggag
     1021 tcgcataagg gagagcgtcg agcaagacgt ttcccgttga atatggctca taacacccct
     1081 tgtattactg tttatgtaag cagacagttt tattgttcat gatgatatat ttttatcttg
     1141 tgcaatgtaa catcagagat tttgagacac aacgtggctt tgttgaataa atcgaacttt
     1201 tgctgagttg aaggatcaga tcacgcatct tcccgacaac gcagaccgtt ccgtggcaaa
     1261 gcaaaagttc aaaatcacca actggtccac ctacaacaaa gctctcatca accgtggctc
     1321 cctcactttc tggctggatg atggggcgat tcaggcctgg tatgagtcag caacaccttc
     1381 ttcacgaggc agacctcagc gctattctga ccttgccatc acgactgtgc tggtcattaa
     1441 acgcgtattc aggctgaccc tgcgcgctgc gcagggcttt attgattcca tttttacact
     1501 gatgaatgtt ccgttgcgct gcccggatta cagctagaag gaccaccttt gattgtaaat
     1561 agtaataatt accaccctta tttaattatt tatttaactt atttatttat ttattctaca
     1621 tatatacaaa tctaataaag tgaaaatctc ccccttcaca tttataattg gccagtcttt
     1681 ttcaaataag cattccaacc agcatttcta taccttttac cttcaatatc tggatctctt
     1741 cctttaccaa acaatcctct accaacaatg ataatatcag ttccagtgct aacaacttca
     1801 tcaacagttc tatattgttg tcctaatcca tcacctttat catctaatcc aactccaggt
     1861 gtcataataa gccaatcaaa tccttcttct tggccaccca tatcacgttg ggcaataaat
     1921 ccaataacaa attccttatc ggatttagca atttcaacag ttttttgaga atattctcca
     1981 tatgctaatg atcccactga tgataattca gctaacatca ataaccctct tggctcttgg
     2041 ttggtggtgg tttctttagc tccctgtttt aatccttcaa ccactccatt cccagtgaca
     2101 ccatgagcat tggtaatatc tgcccaacta ctaattttat aaactccacc aatatattgt
     2161 ttctttacgg tattaccaat atcagcaaat tttctatctt caaaaatcat aaattgatgt
     2221 ttacgtgaaa gttctaataa tggttcaata gtggattcat aggaaaaatc attgattata
     2281 tcaatatgag tcttgattaa gcatacataa ggacctaatt tatcaattaa ttcaaggaat
     2341 tccttagtgg tatcaacgtc aattgatgca cataaattgg ttttcttcag ttccattaat
     2401 cgaaataatc gctgtgctac tggtgaggca tgagtttctg ctctctcact ataggtctta
     2461 gtgttgactg tcatatcttt tgtttgctat ttctatttct atttctttct tcaactgtaa
     2521 aaggggcggt atataaacca gtgtgtatgg ggttgttgct caggaattga tcaatgttta
     2581 aaagaatggt tgaaatcatc attgaagaac aaaaaaaaag attttttctt tcattatgtt
     2641 atctgtataa tatgcaggaa ataagattgc aaaaaaatag ccgttttttt ggatactatc
     2701 aaacaagagg aatgactaat acagaattag tgttacgaat caatggcact acagcaactt
     2761 tcaacaaaat aatctacgtt caaaatataa tatatactat taaataaaat caaataataa
     2821 aataatttta aagaaagtag taccagctct tttttttgtt tccgtttata ccatccaaat
     2881 caattcctat tagtactcaa ccaagtcatt ctgagaatag tgtatgcggc gaccgagttg
     2941 ctcttgcccg gcgtcaacac gggataatac cgcgccacat agcagaactt taaaagtgct
     3001 catcattgga aaacgttctt cggggcgaaa actctcaagg atcttaccgc tgttgagatc
     3061 cagttcgatg taacccactc gtgcacccaa ctgatcttca gcatctttta ctttcaccag
     3121 cgtttctggg tgagcaaaaa caggaaggca aaatgccgca aaaaagggaa taagggcgac
     3181 acggaaatgt tgaatactca tactcttcct ttttcaatat tattgaagca tttatcaggg
     3241 ttattgtctc atgagcggat acatatttga atgtatttag aaaaataaac aaataggggt
     3301 tccgcgcaca tttccccgaa aagtgccacc tgacgtctaa gaaaccatta ttatcatgac
     3361 attaacctat aaaaataggc gtatcacgag gccctttcgt cttcaagaat tctcatgttt
     3421 gacagcttat catcgcgcca ttcgccattc aggctgcgca actgttggga agggcgatcg
     3481 gtgcgggcct cttcgctatt acgccagctg gcgaaagggg gatgtgctgc aaggcgatta
     3541 agttgggtaa cgccagggtt ttcccagtca cgacgttgta aaacgacggc cagtgaattc
     3601 ccggggatcc agacttgtta aatctgatgt tttgacgatt atcattgaat gtaagatcaa
     3661 taaactattc tctttctttt tatttcaaaa ccttgcagta ccatggaatt atatcaagcc
     3721 atgttttaaa ttgatcagta gtctaatcgg gcttcaaggg aaaaggtggt ttactcgctt
     3781 aacgatactt ttgacatttc tactacttag attgtaagag agcattggta gcatcaaaaa
     3841 cacacaaaat ataatcattt tcccaattgg gaacaattca aaataaaaac aactttgatg
     3901 cgaagaactt caatatttta gctaattata tcaaaattgg aatggagtct ctactactac
     3961 atgcttttct caatgaaatt acgttaaaac ttttctcgtc ttcttccatt tttttttata
     4021 ttctttactt tttctttaat ttttttttgt cattccttat ttaaatttcc agaataccct
     4081 ttaaactaca taatatcatg aagaatccat ttgacagtgg cagtgacgat gaagatccat
     4141 ttcttagtaa tccacaatct gcaccatcaa tgccctacgc agcatatact agtgacagaa
     4201 catcgccccg caagacatac caaccattga attttgacag tgaggacgaa gatgctaaag
     4261 aaagcgaatt tatggctttc ccactgtcga cctgcaggca tgcaagcttg gcgtaatcat
     4321 ggtcatagct gtttcctgtg tgaaattgtt atccgctcac aattccacac aacatacgag
     4381 ccggaagcat aaagtgtaaa gcctggggtg cctaatgagt gagctaactc acattaattg
     4441 cgttgcgctc actgcccgct ttccagtcgg gaaacctgtc gtgccagctg cattaatgaa
     4501 tcggccaacg cgcggggaga ggcggtttgc gtattgggcg ctcttccgct tcctcgctca
     4561 ctgactcgct gcgctcggtc gttcggctgc ggcgagcggt atcagctcac tcaaaggcgg
     4621 taatacggtt atccacagaa tcaggggata acgcaggaaa gaacatgtga gcaaaaggcc
     4681 agcaaaaggc caggaaccgt aaaaaggccg cgttgctggc gtttttccat aggctccgcc
     4741 cccctgacga gcatcacaaa aatcgacgct caagtcagag gtggcgaaac ccgacaggac
     4801 tataaagata ccaggcgttt ccccctggaa gctccctcgt gcgctctcct gttccgaccc
     4861 tgccgcttac cggatacctg tccgcctttc tcccttcggg aagcgtggcg ctttctcata
     4921 gctcacgctg taggtatctc agttcggtgt aggtcgttcg ctccaagctg ggctgtgtgc
     4981 acgaaccccc cgttcagccc gaccgctgcg ccttatccgg taactatcgt cttgagtcca
     5041 acccggtaag acacgactta tcgccactgg cagcagccac tggtaacagg attagcagag
     5101 cgaggtatgt aggcggtgct acagagttct tgaagtggtg gcctaactac ggctacacta
     5161 gaaggacagt atttggtatc tgcgctctgc tgaagccagt taccttcgga aaaagagttg
     5221 gtagctcttg atccggcaaa caaaccaccg ctggtagcgg tggttttttt gtttgcaagc
     5281 agcagattac gcgcagaaaa aaaggatctc aagaagatcc tttgatcttt tctacggggt
     5341 ctgacgctca gtggaacgaa aactcacgtt aagggatttt ggtcatgaga ttatcaaaaa
     5401 ggatcttcac ctagatcctt ttaaattaaa aatgaagttt taaatcaatc taaagtatat
     5461 atgagtaaac ttggtctgac agttaccaat gcttaatcag tgaggcacct atctcagcga
     5521 tctgtctatt tcgttcatcc atagttgcct gactccccgt cgtgtagata actacgatac
     5581 gggagggctt accatctggc cccagtgctg caatgatacc gcgagaccca cgctcaccgg
     5641 ctccagattt atcagcaata aaccagccag ccggaagggc cgagcgcaga agtggtcctg
     5701 caactttatc cgcctccatc cagtctatta attgttgccg ggaagctaga gtaagtagtt
     5761 cgccagttaa tagtttgcgc aacgttgttg ccattgctac aggcatcgtg gtgtcacgct
     5821 cgtcgtttgg tatggcttca ttcagctccg gttcccaacg atcaaggcga gttacatgat
     5881 cccccatgtt gtgcaaaaaa gcggttagct ccttcggtcc tccgatcgtt gtcagaagta
     5941 agttggccgc agtgttatca ctcatggtta tggcagcact gcataattct cttactgtca
     6001 tgccatccgt aagatgcttt tctgtgactg gtgagtactc aaccaagtca ttctgagaat
     6061 agtgtatgcg gcgaccgagt tgctcttgcc cggcgtcaat acgggataat accgcgccac
     6121 atagcagaac tttaaaagtg ctcatcattg gaaaacgttc ttcggggcga aaactctcaa
     6181 ggatcttacc gctgttgaga tccagttcga tgtaacccac tcgtgcaccc aactgatctt
     6241 cagcatcttt tactttcacc agcgtttctg ggtgagcaaa aacaggaagg caaaatgccg
     6301 caaaaaaggg aataagggcg acacggaaat gttgaatact catactcttc ctttttcaat
     6361 attattgaag catttatcag ggttattgtc tcatgagcgg atacatattt gaatgtattt
     6421 agaaaaataa acaaataggg gttccgcgca catttccccg aaaagtgcca cctgacgtct
     6481 aagaaaccat tattatcatg acattaacct ataaaaatag gcgtatcacg aggccctttc
     6541 gtc
//
