LOCUS       V003679                 9021 bp    DNA     circular SYN 18-DEC-2018
DEFINITION  Exported.
ACCESSION   V003679
VERSION     V003679
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
            .
REFERENCE   1  (bases 1 to 9021)
  AUTHORS   De Schamphelaire W, Olbrechts A, Meert J, Verhelst K, Roggeman
            Fonseca M, Vanhoucke M, Beyaert R.
  TITLE     BCCM/LMBP Plasmid collection
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 9021)
  AUTHORS   De Schamphelaire W.
  TITLE     Direct Submission
  JOURNAL   Submitted (02-FEB-2017) BCCM/LMBP, Universiteit Gent,
            Technologiepark 927, 9052, BELGIUM
REFERENCE   3  (bases 1 to 9021)
  TITLE     Direct Submission
REFERENCE   4  (bases 1 to 9021)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName:
            "Unpublished"
            SGRef: number: 2; type: "Journal Article"; journalName: "Submitted
            (02-FEB-2017) BCCM/LMBP, Universiteit Gent, Technologiepark 927,
            9052, BELGIUM"
            SGRef: number: 3; type: "Journal Article"
FEATURES             Location/Qualifiers
     source          1..9021
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     CDS             complement(520..1638)
                     /gene="LEU2"
                     /label="3-isopropylmalate dehydrogenase"
                     /note="3-isopropylmalate dehydrogenase from Candida
                     albicans. Accession#: P87186"
     rep_origin      complement(2562..3230)
                     /direction=LEFT
                     /label="Candida ARS2"
                     /note="Candida ARS2"
     CDS             complement(4143..4952)
                     /gene="URA3"
                     /label="Orotidine 5'-phosphate decarboxylase"
                     /note="Orotidine 5'-phosphate decarboxylase from Candida
                     albicans (strain SC5314 / ATCC MYA-2876). Accession#:
                     P13649"
     promoter        complement(5680..5784)
                     /gene="bla"
                     /label="AmpR promoter"
     primer_bind     6056..6072
                     /label="M13 fwd"
                     /note="common sequencing primer, one of multiple similar
                     variants"
     rep_origin      6092..6732
                     /label="Candida ARS3"
                     /note="Candida ARS3"
     primer_bind     complement(6800..6816)
                     /label="M13 rev"
                     /note="common sequencing primer, one of multiple similar
                     variants"
     protein_bind    complement(6824..6840)
                     /label="lac operator"
                     /note="The lac repressor binds to the lac operator to
                     inhibit transcription in E. coli. This inhibition can be
                     relieved by adding lactose or
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(6848..6878)
                     /label="lac promoter"
                     /note="promoter for the E. coli lac operon"
     protein_bind    complement(6893..6914)
                     /label="CAP binding site"
                     /note="CAP binding activates transcription in the presence
                     of cAMP."
     rep_origin      complement(7202..7790)
                     /direction=LEFT
                     /label="ori"
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
                     replication"
     CDS             complement(7964..8821)
                     /label="AmpR"
                     /note="beta-lactamase"
     promoter        complement(8822..8926)
                     /label="AmpR promoter"
ORIGIN
        1 tcgcgcgttt cggtgatgac ggtgaaaacc tctgacacat gcagctcccg gagacggtca
       61 cagcttgtct gtaagcggat gccgggagca gacaagcccg tcagggcgcg tcagcgggtg
      121 ttggcgggtg tcggggctgg cttaactatg cggcatcaga gcagattgta ctgagagtgc
      181 accataaatt cagtcagttt attgacaacg aatttggaaa caagcccgtg cccaagttgt
      241 cgaaacctct gttgcctcac aataccagcg ctataacatt gacgagcagt agtgaggaga
      301 agagtttttc tctccgaatg aagagccaaa tcatatacga gtgccaagtc aatccgaatc
      361 aagcgaggcc accattacat ctaccaggca aaaaaatttt gcaaataagc aacctttatt
      421 gagtgaagat tcggtaactc agttgtgtgc atatttagag aaatctgtag actttggagt
      481 atttgaaatt taaatgctaa ctactgtata tactgtttat gtattttgtt ttaaaatttt
      541 agtgactgtt tcggctattg cgtcaccgac ttctgtagta gagctagtac ctcttaaatc
      601 tgctgttctg ataccgctat ccaaaacctg tttgacagct tcttccaatg cttcagtttc
      661 cttaacacaa tccaaggcta atctcaacat ggaagcggca gacaagattg tagcaattgg
      721 attcactttg ttggctggta aatctggagc agacccatgg caaggctcat aaagaccaaa
      781 agcagtgttg gtatctggca atgaagccaa tgaagcagat ggcaataaac ccaaagatcc
      841 tgggataacg gatgcctcat ctgagatgat atcaccaaac atgttagatg taatgatgat
      901 accgctcaat ttggttgggt tttggatcaa aatcattgca gccgaatcaa tcaattgatg
      961 ctgcacagac aaagctggaa attcttcaga aatcacttta tcaacggtct ttctccacaa
     1021 tctcgacgat gccaaaacat tagctttgtc caaagaccat attggcaatg gtggattgtg
     1081 ctgcaaagcc ataaatgctg ccatacgagt gatacgagta acttcgtcaa cagtatactt
     1141 tggtctatac caagctgttt ggtggtcttc actttcttct tgttcttgac gttcaccaaa
     1201 atatatacca ccaactaatt cacgaacaat aattagattg gttcctttga ccacttcagc
     1261 ttttaatgga gacaattcta ataacgaatc actggcaaag ttacatggtc taatgttggc
     1321 ataaaggttc aattctttac ggattttcaa caatccttgt tcaggacgaa cagtgccagt
     1381 accccatttg ggtccaccaa cagcacccaa caaaactgca tcggatgatt tagcactttc
     1441 aagagcatca tctggaagcg ggacgccagt ggcatcaatg gcagcaccac caatcaagtg
     1501 gtgtttgaaa tcaaattgga ttttttggta gggagtggga gcttcaattg ctttcaagac
     1561 tttggttggt tcattgacaa tttcggtacc gacatggtca ccaggcaaga cagtaatggt
     1621 ttgggtttta acagacatgt ttttgggata ttggttttaa aagaaaggaa taagaaaagg
     1681 ggagtatttc tggagtgaaa aaaaaaaatt catttgttga gaaaagggca ccccagaagg
     1741 agcgccggaa tagcaaaatc acaacctcat gctcgctccg gtacaggcaa aaaaaaaaaa
     1801 ttattcaaag taatctcttt ctatacatga taaatacacc aacaaaaact acctaatgac
     1861 cactccattc aattgtattc aaatcaatac cttcttgaac catttcccat aacggggaca
     1921 tttttcttaa aaagtcaact ctttcattaa ttgcttgaat gacatagtcg acttcggctt
     1981 cagttgtaaa tctaccaatc ccaaatctaa ttgaagaatg agccaaggca tcatcagctc
     2041 ccaatgcatg caaaacatat gatggttcca aagatgccga agtacatgct gacccagaac
     2101 tcaaagcaat atctttcaag gccattaaca acgattcacc ttcaatgtaa gcaaaagata
     2161 cattcacgca accagggtat tgagatttgg cgtcgttaca accgttaaat tgggtagatg
     2221 gaattgacaa caatccattt ttcaacttag aggataattt ctcaatgtgt cttttgtcaa
     2281 acagcgattc ttgcttcatt aatcttgctg cttcaccaaa cccagcaact aatggaggag
     2341 ctaaagtacc tgaacgcaaa cctctttctt gaccaccacc agtaatgatt ggatctatgc
     2401 ggtgtgaaat accgcacaga tgcgtaagga gaaaataccg catcaggcgc gatagattgt
     2461 cgcacctgat tgcccgacat tatcgcgagc ccatttatac ccatataaat cagcatccat
     2521 gttggaattt aatcgcggcc gctacgaatg ttagagacaa aatgtagtcc aggaccggcc
     2581 agacgcagtg tgcgtgctgg ttgtgcaatt atactagcac atctaggtgt tttaggaaga
     2641 aacgtccacc accaaaaaat taatttccaa atattggcac ttttattaca ccagtggtgt
     2701 tacacagccc cccaaacact atcccggttg ttagattgaa gtttgtttaa caaaaattgg
     2761 aagttctatt ttttactttt tgtacaaaat ttggcaagaa aattggagta aaatattttt
     2821 atataaattt caaactaaaa atatccaaaa cataaaataa tacacaaaat gagatgctct
     2881 atttagccaa accaaccatt acgggtgtgt tgtttggtgt tgtctgacca tgggtatacc
     2941 atttgttagt gtatagctgc actgttttat gtttttcgat ttttccacat aaaaggagta
     3001 ctcctatact aataacaaac actccactat aattggcaac cacaattatg cctggggaaa
     3061 tattttccac tccgagtgta catacatttt tggtgtgttg ttggtggttg aaaattgtgt
     3121 gggtcacgtg catttaatta gggttagggc tacggtttgt gtgcagtacc acgttcaaat
     3181 tgaggctctc tggtataact tgcactacct catcgctacc ccggattttt ttttggtatg
     3241 atcctctacg ccggacgcat cgtggccggc atcaccggcg ccacaggtgc ggttgctggc
     3301 gcctatatcg ccgacatcac cgatggggaa gatcgggctc gccacttcgg gctcatgagc
     3361 gcttgtttcg gcgtgggtaa ggtggcaggc tcggtggccg ggggactgtt gggcgccatc
     3421 tccttgcatg caccattcct tgcggcggcg gtgctcaacg gcctcaacct actactgggc
     3481 tgcttcctaa tgcaggagtc gcataaggga gagcgtcgag caagacgttt cccgttgaat
     3541 atggctcata acaccccttg tattactgtt tatgtaagca gacagtttta ttgttcatga
     3601 tgatatattt ttatcttgtg caatgtaaca tcagagattt tgagacacaa cgtggctttg
     3661 ttgaataaat cgaacttttg ctgagttgaa ggatcagatc acgcatcttc ccgacaacgc
     3721 agaccgttcc gtggcaaagc aaaagttcaa aatcaccaac tggtccacct acaacaaagc
     3781 tctcatcaac cgtggctccc tcactttctg gctggatgat ggggcgattc aggcctggta
     3841 tgagtcagca acaccttctt cacgaggcag acctcagcgc tattctgacc ttgccatcac
     3901 gactgtgctg gtcattaaac gcgtattcag gctgaccctg cgcgctgcgc agggctttat
     3961 tgattccatt tttacactga tgaatgttcc gttgcgctgc ccggattaca gctagaagga
     4021 ccacctttga ttgtaaatag taataattac cacccttatt taattattta tttaacttat
     4081 ttatttattt attctacata tatacaaatc taataaagtg aaaatctccc ccttcacatt
     4141 tataattggc cagtcttttt caaataagca ttccaaccag catttctata ccttttacct
     4201 tcaatatctg gatctcttcc tttaccaaac aatcctctac caacaatgat aatatcagtt
     4261 ccagtgctaa caacttcatc aacagttcta tattgttgtc ctaatccatc acctttatca
     4321 tctaatccaa ctccaggtgt cataataagc caatcaaatc cttcttcttg gccacccata
     4381 tcacgttggg caataaatcc aataacaaat tccttatcgg atttagcaat ttcaacagtt
     4441 ttttgagaat attctccata tgctaatgat cccactgatg ataattcagc taacatcaat
     4501 aaccctcttg gctcttggtt ggtggtggtt tctttagctc cctgttttaa tccttcaacc
     4561 actccattcc cagtgacacc atgagcattg gtaatatctg cccaactact aattttataa
     4621 actccaccaa tatattgttt ctttacggta ttaccaatat cagcaaattt tctatcttca
     4681 aaaatcataa attgatgttt acgtgaaagt tctaataatg gttcaatagt ggattcatag
     4741 gaaaaatcat tgattatatc aatatgagtc ttgattaagc atacataagg acctaattta
     4801 tcaattaatt caaggaattc cttagtggta tcaacgtcaa ttgatgcaca taaattggtt
     4861 ttcttcagtt ccattaatcg aaataatcgc tgtgctactg gtgaggcatg agtttctgct
     4921 ctctcactat aggtcttagt gttgactgtc atatcttttg tttgctattt ctatttctat
     4981 ttctttcttc aactgtaaaa ggggcggtat ataaaccagt gtgtatgggg ttgttgctca
     5041 ggaattgatc aatgtttaaa agaatggttg aaatcatcat tgaagaacaa aaaaaaagat
     5101 tttttctttc attatgttat ctgtataata tgcaggaaat aagattgcaa aaaaatagcc
     5161 gtttttttgg atactatcaa acaagaggaa tgactaatac agaattagtg ttacgaatca
     5221 atggcactac agcaactttc aacaaaataa tctacgttca aaatataata tatactatta
     5281 aataaaatca aataataaaa taattttaaa gaaagtagta ccagctcttt tttttgtttc
     5341 cgtttatacc atccaaatca attcctatta gtactcaacc aagtcattct gagaatagtg
     5401 tatgcggcga ccgagttgct cttgcccggc gtcaacacgg gataataccg cgccacatag
     5461 cagaacttta aaagtgctca tcattggaaa acgttcttcg gggcgaaaac tctcaaggat
     5521 cttaccgctg ttgagatcca gttcgatgta acccactcgt gcacccaact gatcttcagc
     5581 atcttttact ttcaccagcg tttctgggtg agcaaaaaca ggaaggcaaa atgccgcaaa
     5641 aaagggaata agggcgacac ggaaatgttg aatactcata ctcttccttt ttcaatatta
     5701 ttgaagcatt tatcagggtt attgtctcat gagcggatac atatttgaat gtatttagaa
     5761 aaataaacaa ataggggttc cgcgcacatt tccccgaaaa gtgccacctg acgtctaaga
     5821 aaccattatt atcatgacat taacctataa aaataggcgt atcacgaggc cctttcgtct
     5881 tcaagaattc tcatgtttga cagcttatca tcgcgccatt cgccattcag gctgcgcaac
     5941 tgttgggaag ggcgatcggt gcgggcctct tcgctattac gccagctggc gaaaggggga
     6001 tgtgctgcaa ggcgattaag ttgggtaacg ccagggtttt cccagtcacg acgttgtaaa
     6061 acgacggcca gtgaattccc ggggatccag acttgttaaa tctgatgttt tgacgattat
     6121 cattgaatgt aagatcaata aactattctc tttcttttta tttcaaaacc ttgcagtacc
     6181 atggaattat atcaagccat gttttaaatt gatcagtagt ctaatcgggc ttcaagggaa
     6241 aaggtggttt actcgcttaa cgatactttt gacatttcta ctacttagat tgtaagagag
     6301 cattggtagc atcaaaaaca cacaaaatat aatcattttc ccaattggga acaattcaaa
     6361 ataaaaacaa ctttgatgcg aagaacttca atattttagc taattatatc aaaattggaa
     6421 tggagtctct actactacat gcttttctca atgaaattac gttaaaactt ttctcgtctt
     6481 cttccatttt tttttatatt ctttactttt tctttaattt ttttttgtca ttccttattt
     6541 aaatttccag aatacccttt aaactacata atatcatgaa gaatccattt gacagtggca
     6601 gtgacgatga agatccattt cttagtaatc cacaatctgc accatcaatg ccctacgcag
     6661 catatactag tgacagaaca tcgccccgca agacatacca accattgaat tttgacagtg
     6721 aggacgaaga tgctaaagaa agcgaattta tggctttccc actgtcgacc tgcaggcatg
     6781 caagcttggc gtaatcatgg tcatagctgt ttcctgtgtg aaattgttat ccgctcacaa
     6841 ttccacacaa catacgagcc ggaagcataa agtgtaaagc ctggggtgcc taatgagtga
     6901 gctaactcac attaattgcg ttgcgctcac tgcccgcttt ccagtcggga aacctgtcgt
     6961 gccagctgca ttaatgaatc ggccaacgcg cggggagagg cggtttgcgt attgggcgct
     7021 cttccgcttc ctcgctcact gactcgctgc gctcggtcgt tcggctgcgg cgagcggtat
     7081 cagctcactc aaaggcggta atacggttat ccacagaatc aggggataac gcaggaaaga
     7141 acatgtgagc aaaaggccag caaaaggcca ggaaccgtaa aaaggccgcg ttgctggcgt
     7201 ttttccatag gctccgcccc cctgacgagc atcacaaaaa tcgacgctca agtcagaggt
     7261 ggcgaaaccc gacaggacta taaagatacc aggcgtttcc ccctggaagc tccctcgtgc
     7321 gctctcctgt tccgaccctg ccgcttaccg gatacctgtc cgcctttctc ccttcgggaa
     7381 gcgtggcgct ttctcatagc tcacgctgta ggtatctcag ttcggtgtag gtcgttcgct
     7441 ccaagctggg ctgtgtgcac gaaccccccg ttcagcccga ccgctgcgcc ttatccggta
     7501 actatcgtct tgagtccaac ccggtaagac acgacttatc gccactggca gcagccactg
     7561 gtaacaggat tagcagagcg aggtatgtag gcggtgctac agagttcttg aagtggtggc
     7621 ctaactacgg ctacactaga aggacagtat ttggtatctg cgctctgctg aagccagtta
     7681 ccttcggaaa aagagttggt agctcttgat ccggcaaaca aaccaccgct ggtagcggtg
     7741 gtttttttgt ttgcaagcag cagattacgc gcagaaaaaa aggatctcaa gaagatcctt
     7801 tgatcttttc tacggggtct gacgctcagt ggaacgaaaa ctcacgttaa gggattttgg
     7861 tcatgagatt atcaaaaagg atcttcacct agatcctttt aaattaaaaa tgaagtttta
     7921 aatcaatcta aagtatatat gagtaaactt ggtctgacag ttaccaatgc ttaatcagtg
     7981 aggcacctat ctcagcgatc tgtctatttc gttcatccat agttgcctga ctccccgtcg
     8041 tgtagataac tacgatacgg gagggcttac catctggccc cagtgctgca atgataccgc
     8101 gagacccacg ctcaccggct ccagatttat cagcaataaa ccagccagcc ggaagggccg
     8161 agcgcagaag tggtcctgca actttatccg cctccatcca gtctattaat tgttgccggg
     8221 aagctagagt aagtagttcg ccagttaata gtttgcgcaa cgttgttgcc attgctacag
     8281 gcatcgtggt gtcacgctcg tcgtttggta tggcttcatt cagctccggt tcccaacgat
     8341 caaggcgagt tacatgatcc cccatgttgt gcaaaaaagc ggttagctcc ttcggtcctc
     8401 cgatcgttgt cagaagtaag ttggccgcag tgttatcact catggttatg gcagcactgc
     8461 ataattctct tactgtcatg ccatccgtaa gatgcttttc tgtgactggt gagtactcaa
     8521 ccaagtcatt ctgagaatag tgtatgcggc gaccgagttg ctcttgcccg gcgtcaatac
     8581 gggataatac cgcgccacat agcagaactt taaaagtgct catcattgga aaacgttctt
     8641 cggggcgaaa actctcaagg atcttaccgc tgttgagatc cagttcgatg taacccactc
     8701 gtgcacccaa ctgatcttca gcatctttta ctttcaccag cgtttctggg tgagcaaaaa
     8761 caggaaggca aaatgccgca aaaaagggaa taagggcgac acggaaatgt tgaatactca
     8821 tactcttcct ttttcaatat tattgaagca tttatcaggg ttattgtctc atgagcggat
     8881 acatatttga atgtatttag aaaaataaac aaataggggt tccgcgcaca tttccccgaa
     8941 aagtgccacc tgacgtctaa gaaaccatta ttatcatgac attaacctat aaaaataggc
     9001 gtatcacgag gccctttcgt c
//
