LOCUS       V003686                 7120 bp    DNA     circular SYN 18-DEC-2018
DEFINITION  Exported.
ACCESSION   V003686
VERSION     V003686
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
            .
REFERENCE   1  (bases 1 to 7120)
  AUTHORS   Redder P, Linder P.
  TITLE     New Range of Vectors with a Stringent 5-Fluoroorotic Acid-Based
            Counterselection System for Generating Mutants by Allelic
            Replacement in Staphylococcus aureus
  JOURNAL   Appl. Environ. Microbiol. 78 (11), 3846-3854 (2012)
   PUBMED   22447609
REFERENCE   2  (bases 1 to 7120)
  AUTHORS   Redder P, Linder P.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2012) Department of Microbiology and Molecular
            Medicine, University of Geneva, Faculty of Medicine, Rue
            Michel-Servet 1, Geneva 1203, Switzerland
REFERENCE   3  (bases 1 to 7120)
  TITLE     Direct Submission
REFERENCE   4  (bases 1 to 7120)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     ##Assembly-Data-START##
            Assembly Method       :: BioEdit, CAP v. 7.0.9.0
            Sequencing Technology :: Sanger dideoxy sequencing
            ##Assembly-Data-END##
            SGRef: number: 1; type: "Journal Article"; journalName: "Appl.
            Environ. Microbiol."; date: "2012"; volume: "78"; issue: "11";
            pages: "3846-3854"
            SGRef: number: 2; type: "Journal Article"; journalName: "Submitted
            (12-MAR-2012) Department of Microbiology and Molecular Medicine,
            University of Geneva, Faculty of Medicine, Rue Michel-Servet 1,
            Geneva 1203, Switzerland"
            SGRef: number: 3; type: "Journal Article"
FEATURES             Location/Qualifiers
     source          1..7120
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     379..395
                     /label="M13 fwd"
                     /note="common sequencing primer, one of multiple similar
                     variants"
     misc_feature    396..452
                     /label="MCS"
                     /note="pUC18/19 multiple cloning site"
     primer_bind     complement(465..481)
                     /label="M13 rev"
                     /note="common sequencing primer, one of multiple similar
                     variants"
     protein_bind    complement(489..505)
                     /label="lac operator"
                     /note="The lac repressor binds to the lac operator to
                     inhibit transcription in E. coli. This inhibition can be
                     relieved by adding lactose or
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(513..543)
                     /label="lac promoter"
                     /note="promoter for the E. coli lac operon"
     protein_bind    complement(558..579)
                     /label="CAP binding site"
                     /note="CAP binding activates transcription in the presence
                     of cAMP."
     CDS             complement(717..1364)
                     /gene="pyrE"
                     /label="Orotate phosphoribosyltransferase"
                     /note="Orotate phosphoribosyltransferase from Bacillus
                     subtilis (strain 168). Accession#: P25972"
     CDS             complement(1346..2062)
                     /gene="pyrF"
                     /label="Orotidine 5'-phosphate decarboxylase"
                     /note="Orotidine 5'-phosphate decarboxylase from Bacillus
                     subtilis (strain 168). Accession#: P25971"
     CDS             complement(2410..3141)
                     /gene="ermC"
                     /label="rRNA adenine N-6-methyltransferase"
                     /note="rRNA adenine N-6-methyltransferase from
                     Staphylococcus aureus. Accession#: P02979"
     rep_origin      complement(3539..5160)
                     /direction=LEFT
                     /note="thermosensitive origin of replication; for
                     replication in Staphylococcus aureus.; pT181ts"
     rep_origin      complement(5301..5889)
                     /direction=LEFT
                     /label="ori"
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
                     replication"
     CDS             complement(6063..6920)
                     /label="AmpR"
                     /note="beta-lactamase"
     promoter        complement(6921..7025)
                     /label="AmpR promoter"
ORIGIN
        1 tcgcgcgttt cggtgatgac ggtgaaaacc tctgacacat gcagctcccg gagacggtca
       61 cagcttgtct gtaagcggat gccgggagca gacaagcccg tcagggcgcg tcagcgggtg
      121 ttggcgggtg tcggggctgg cttaactatg cggcatcaga gcagattgta ctgagagtgc
      181 accatatgcg gtgtgaaata ccgcacagat gcgtaaggag aaaataccgc atcaggcgcc
      241 attcgccatt caggctgcgc aactgttggg aagggcgatc ggtgcgggcc tcttcgctat
      301 tacgccagct gtcgaatggg ggatgtgctg caaggcgatt aagttgggta acgccagggt
      361 tttcccagtc acgacgttgt aaaacgacgg ccagtgaatt cgagctcggt acccggggat
      421 cctctagagt cgacctgcag gcatgcaagc ttggcgtaat catggtcata gctgtttcct
      481 gtgtgaaatt gttatccgct cacaattcca cacaacatac gagccggaag cataaagtgt
      541 aaagcctggg gtgcctaatg agtgagctaa ctcacattaa ttgcgttgcg ctcactgccc
      601 gctttccagt cgggaaccac tcgagccatg gattaataaa aaaacaggcc tcacagcctg
      661 ttttcaagta cagcaaggct ttgaagcctc tttacatcat ttgaatttat tttttatttt
      721 ttaaaccaat cttttgactc gggatttcgt ttccatgttt gcagcttttt tagatcatct
      781 gaatgaatat ttccgttttc aagcgcgacc tctgtgagcg tatcataatc ggttaatgag
      841 tagtatggca gttctgcctt tgcgaaggct tcctccgctt taggaagtcc gtacgtaaag
      901 attgagacga caccaagcac ttcacagccg gccgcttgta aagctgcaca ggcttcaagc
      961 acgctgcctc ctgtggaaat taagtcttca atgacgactg ttttttgccc ttcttgcaca
     1021 gctccctcaa tctgattgcc ttttccgtgc gccttcggct tgctcctcac ataacacatc
     1081 ggaagattca aatggtccgc cgcaagagca gcatgaggaa taccggcagt tgctgttccc
     1141 gcaatcattt cagcttcagg aaaatgctct ttaacaagtt tgctgatacc tgaagcaaca
     1201 tcgtttctga cctctgggaa agatagcgta aggcggttgt cacagtagat cggtgataaa
     1261 atgccgcttg cccatgtgaa cggctcgttc gggcgtaaaa atacagcttg gatgtctaat
     1321 agatgttttg cgatgatttg tttcaagatt tgattccctc ccattcaagt ctgacagcct
     1381 tataggcttt taccgggtct tccgcttttg taatcgagcg tcctactaca atcgctgatg
     1441 aacctttctc tcttgcaatg gcaggcgtcg ctacgcgaac ttggtcattc gcagcgtcct
     1501 ctgacattct gatccccgga gtgacagtca gaaatgaagg cgacaccgct tggtaaatgg
     1561 cttttgcttc atgaacagag cagaccactc catccagtcc gctttcttcc gcctgtttgc
     1621 tgtagtgcac aaccgtatca atcagagact tttcgatcag cagttcatct ttcatgattt
     1681 gctcagatgt gcttgtcagc tgggttaccg cgataagtga cggacgtttt tttccagccg
     1741 gcgtaccttc ttctaagcct tcgagagctg cctgcatcat ttttttgccc ccggcagcat
     1801 gaacattgac gaggtctact ccaagactgg caaggcgctt catcgctttg tttacagtag
     1861 tcgggatgtc atgaagtttt aagtctaaaa atagctcgca gtttctttct tttagttgtt
     1921 tcacgataga tggcccttct tgataaaaaa gctccatccc aacctttaca aataacggtt
     1981 cttgctgaaa aggcgctaag aacgcaagtg tttcttcagc tgacgcaaaa tcaagcgcga
     2041 tgatgggcag gttgtttttc atgattccag ctccttccga ttccatacat tatgccacac
     2101 cttgtagata aagtcaacaa ctttttgcaa aatttttcag gaattttagc agaggttgtt
     2161 ctggatgtag aacaaaacat ctttccgctc ttgtgctgtt aggatatctt tcttggaagc
     2221 taggtaggca agggctacct caaataaatc ttcttcaggg tgcgctattt ttaaggtgcc
     2281 tagtgaggtc ttgaccactt cacccataat ttcagtgccg aatagtctgg actgggctgt
     2341 gtagatctca caaaaaatag gcacacgaaa aacaagttaa gggatgcagt ttatgcatcc
     2401 cttaacttac ttattaaata atttatagct attgaaaaga gataagaatt gttcaaagct
     2461 aatattgttt aaatcgtcaa ttcctgcatg ttttaaggaa ttgttaaatt gattttttgt
     2521 aaatattttc ttgtattctt tgttaaccca tttcataacg aaataattat acttttgttt
     2581 atctttgtgt gatattcttg attttttcct acttaatctg ataagtgagc tattcacttt
     2641 aggtttagga tgaaaatatt ctcttggaac catacttaat atagaaatat caacttctgc
     2701 cattaaaagt aatgccaatg agcgttttgt atttaataat cttttagcaa acccgtattc
     2761 cacgattaaa taaatctcat tagctatact atcaaaaaca attttgcgta ttatatccgt
     2821 acttatgtta taaggtatat taccatatat tttataggat tggtttttag gaaatttaaa
     2881 ctgcaatata tccttgttta aaacttggaa attatcgtga tcaacaagtt tattttctgt
     2941 agttttgcat aatttatggt ctatttcaat ggcagttacg aaattacacc tctttactaa
     3001 ttcaagggta aaatggcctt ttcctgagcc gatttcaaag atattatcat gttcatttaa
     3061 tcttatattt gtcattattt tatctatatt atgttttgaa gtaataaagt tttgactgtg
     3121 ttttatattt ttctcgttca ttataaccct ctttaatttg gttatatgaa ttttgcttat
     3181 taacgattca ttataaccac ttattttttg tttggttgat aatgaactgt gctgattaca
     3241 aaaatactaa aaatgcccat attttttcct ccttataaaa ttagtataat tatagcacaa
     3301 gctctgataa atatgaacat gatgagtgat cgttaaattt atactgcaat cggatgcgat
     3361 tattgaataa aagatatgag agatttatct aatttctttt ttcttgtaaa aaaagaaagt
     3421 tcttaaagga tctgctgcat taatgaatcg gccaacgcgc ggggagaggc ggtttgcgta
     3481 ttgggcgctc ttccgcttcc tcgctcactg actcgctgcg ctcggtcgtt cggctgcggc
     3541 gcgcctcttt acttgaggtg actaaagttt ataggggtaa attattcttt cacctcatat
     3601 aaattcccca aatttttaaa tagacacttc ataaaaaaat cctcctaaat tagaatagtt
     3661 taaatatatc atttttgttc agtaatatta atatgtacgt acactatttc caaaatttaa
     3721 attcatgttg ccaaaaatcg atttgttttt gcaattgttt ttcgttcgct tttaaagtcg
     3781 atttcattaa gtccgttaaa tcgactggcg aaatttcttt tatcaaattc ttatattttg
     3841 ttctggaatt tctgtgtagc tttccccatt cttcttcatc actcaataac ataaaaacta
     3901 ttgctctatc cgcagtgcgt tggatagttt tccaatctgg ttgcaagata tgtaaatcac
     3961 taaagcaatc attccagtaa tccaccatat ctcttttaaa ttcgatttct acacgccata
     4021 aatgttcaga cataacttca gcatctgcat tatctttacg ttcttgcttt ttattataaa
     4081 ttctaataaa tctattacta tctctcacgc caaaatattt tgtttctggc ttaccattac
     4141 gaccataaaa aatagttttc ttaactgctt tatcagacat tgcatagtag tcactcaaat
     4201 catcttcaaa atcaaaggct aaatctaatc ttgtaaaacc gtcatcttcc atgtagctta
     4261 ttatattttg ttttaaccaa atcatttcat ctcgtgtaag tttgtttgga ttaaattcaa
     4321 tacgcatatt acgtctatcc caactatctg ctttcacttt gtcatattca atataaactt
     4381 tttcttgaag tgctttagct ttaaactttg tttgaagaat atcccaaagt cttatttggg
     4441 gctctacact cataaattta gaaagggctt gagcgttgtc tcggttgaga tttccaacga
     4501 tcgtcattgc atcaaaactt aattttggat cagatatgca cacagtatgt gcgtccaacc
     4561 ggctattaga gtagccggtt ttagaaaaat tgtctaaatc gtgattttcc aaatgatttg
     4621 aatgatttgc atgattgttt ttatacataa aaaatcgact ccttaatctc aatttcgttt
     4681 aaggaatcgc tcacccaaat atatatcttg atgtatattt aaatatcgtt taatatctaa
     4741 atatacaaga ttataaaaac aactcagtgt ttttttcttt gaatgatgtc gttcacaaac
     4801 tttggtcagg tcgtgagcga ctccttttta ttttgttatt aatataacac tatcaaaaga
     4861 tttggtctaa tcagatcaag tcttttttta tttaagcatt tgtattatct ggtaaacagt
     4921 taaaactact aaaacaccaa gtacatactt acttgttata aaattctcaa aggcttctct
     4981 gaatttcatt tcttcaactc cttttattgc tttattccaa tttccttatt ggtcggaacc
     5041 tacagggctt tatgtgttgt tgagttggta ctttcttggg attaatccca attcaagtcc
     5101 aaccaactcg ctaacaagtt agctaacaca tagcccattc caaccaataa gttttctcgg
     5161 cgcgccggca cgcgtatcga tgctcactca aaggcggtaa tacggttatc cacagaatca
     5221 ggggataacg caggaaagaa catgtgagca aaaggccagc aaaaggccag gaaccgtaaa
     5281 aaggccgcgt tgctggcgtt tttccatagg ctccgccccc ctgacgagca tcacaaaaat
     5341 cgacgctcaa gtcagaggtg gcgaaacccg acaggactat aaagatacca ggcgtttccc
     5401 cctggaagct ccctcgtgcg ctctcctgtt ccgaccctgc cgcttaccgg atacctgtcc
     5461 gcctttctcc cttcgggaag cgtggcgctt tctcatagct cacgctgtag gtatctcagt
     5521 tcggtgtagg tcgttcgctc caagctgggc tgtgtgcacg aaccccccgt tcagcccgac
     5581 cgctgcgcct tatccggtaa ctatcgtctt gagtccaacc cggtaagaca cgacttatcg
     5641 ccactggcag cagccactgg taacaggatt agcagagcga ggtatgtagg cggtgctaca
     5701 gagttcttga agtggtggcc taactacggc tacactagaa ggacagtatt tggtatctgc
     5761 gctctgctga agccagttac cttcggaaaa agagttggta gctcttgatc cggcaaacaa
     5821 accaccgctg gtagcggtgg tttttttgtt tgcaagcagc agattacgcg cagaaaaaaa
     5881 ggatctcaag aagatccttt gatcttttct acggggtctg acgctcagtg gaacgaaaac
     5941 tcacgttaag ggattttggt catgagatta tcaaaaagga tcttcaccta gatcctttta
     6001 aattaaaaat gaagttttaa atcaatctaa agtatatatg agtaaacttg gtctgacagt
     6061 taccaatgct taatcagtga ggcacctatc tcagcgatct gtctatttcg ttcatccata
     6121 gttgcctgac tccccgtcgt gtagataact acgatacggg agggcttacc atctggcccc
     6181 agtgctgcaa tgataccgcg agacccacgc tcaccggctc cagatttatc agcaataaac
     6241 cagccagccg gaagggccga gcgcagaagt ggtcctgcaa ctttatccgc ctccatccag
     6301 tctattaatt gttgccggga agctagagta agtagttcgc cagttaatag tttgcgcaac
     6361 gttgttgcca ttgctacagg catcgtggtg tcacgctcgt cgtttggtat ggcttcattc
     6421 agctccggtt cccaacgatc aaggcgagtt acatgatccc ccatgttgtg caaaaaagcg
     6481 gttagctcct tcggtcctcc gatcgttgtc agaagtaagt tggccgcagt gttatcactc
     6541 atggttatgg cagcactgca taattctctt actgtcatgc catccgtaag atgcttttct
     6601 gtgactggtg agtactcaac caagtcattc tgagaatagt gtatgcggcg accgagttgc
     6661 tcttgcccgg cgtcaatacg ggataatacc gcgccacata gcagaacttt aaaagtgctc
     6721 atcattggaa aacgttcttc ggggcgaaaa ctctcaagga tcttaccgct gttgagatcc
     6781 agttcgatgt aacccactcg tgcacccaac tgatcttcag catcttttac tttcaccagc
     6841 gtttctgggt gagcaaaaac aggaaggcaa aatgccgcaa aaaagggaat aagggcgaca
     6901 cggaaatgtt gaatactcat actcttcctt tttcaatatt attgaagcat ttatcagggt
     6961 tattgtctca tgagcggata catatttgaa tgtatttaga aaaataaaca aataggggtt
     7021 ccgcgcacat ttccccgaaa agtgccacct gacgtctaag aaaccattat tatcatgaca
     7081 ttaacctata aaaataggcg tatcacgagg ccctttcgtc
//
