LOCUS       V003694                 8802 bp    DNA     circular SYN 18-DEC-2018
DEFINITION  Exported.
ACCESSION   V003694
VERSION     V003694
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
            .
REFERENCE   1  (bases 1 to 8802)
  AUTHORS   Redder P, Linder P.
  TITLE     New Range of Vectors with a Stringent 5-Fluoroorotic Acid-Based
            Counterselection System for Generating Mutants by Allelic
            Replacement in Staphylococcus aureus
  JOURNAL   Appl. Environ. Microbiol. 78 (11), 3846-3854 (2012)
   PUBMED   22447609
REFERENCE   2  (bases 1 to 8802)
  AUTHORS   Redder P, Linder P.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2012) Department of Microbiology and Molecular
            Medicine, University of Geneva, Faculty of Medicine, Rue
            Michel-Servet 1, Geneva 1203, Switzerland
REFERENCE   3  (bases 1 to 8802)
  TITLE     Direct Submission
REFERENCE   4  (bases 1 to 8802)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     ##Assembly-Data-START##
            Assembly Method       :: BioEdit, CAP v. 7.0.9.0
            Sequencing Technology :: Sanger dideoxy sequencing
            ##Assembly-Data-END##
            SGRef: number: 1; type: "Journal Article"; journalName: "Appl.
            Environ. Microbiol."; date: "2012"; volume: "78"; issue: "11";
            pages: "3846-3854"
            SGRef: number: 2; type: "Journal Article"; journalName: "Submitted
            (12-MAR-2012) Department of Microbiology and Molecular Medicine,
            University of Geneva, Faculty of Medicine, Rue Michel-Servet 1,
            Geneva 1203, Switzerland"
            SGRef: number: 3; type: "Journal Article"
FEATURES             Location/Qualifiers
     source          1..8802
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     379..395
                     /label="M13 fwd"
                     /note="common sequencing primer, one of multiple similar
                     variants"
     CDS             1455..2144
                     /gene="pyrF"
                     /label="Orotidine 5'-phosphate decarboxylase"
                     /note="Orotidine 5'-phosphate decarboxylase from
                     Staphylococcus aureus (strain MSSA476). Accession#: Q6GA09"
     CDS             complement(3895..5271)
                     /gene="tet"
                     /label="Tetracycline resistance protein"
                     /note="Tetracycline resistance protein from Staphylococcus
                     aureus. Accession#: P02983"
     rep_origin      complement(5679..6842)
                     /direction=LEFT
                     /note="thermosensitive origin of replication; for
                     replication in Staphylococcus aureus; pE194ts"
     rep_origin      complement(6983..7571)
                     /direction=LEFT
                     /label="ori"
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
                     replication"
     CDS             complement(7745..8602)
                     /label="AmpR"
                     /note="beta-lactamase"
     promoter        complement(8603..8707)
                     /label="AmpR promoter"
ORIGIN
        1 tcgcgcgttt cggtgatgac ggtgaaaacc tctgacacat gcagctcccg gagacggtca
       61 cagcttgtct gtaagcggat gccgggagca gacaagcccg tcagggcgcg tcagcgggtg
      121 ttggcgggtg tcggggctgg cttaactatg cggcatcaga gcagattgta ctgagagtgc
      181 accatatgcg gtgtgaaata ccgcacagat gcgtaaggag aaaataccgc atcaggcgcc
      241 attcgccatt caggctgcgc aactgttggg aagggcgatc ggtgcgggcc tcttcgctat
      301 tacgccagct gtcgaatggg ggatgtgctg caaggcgatt aagttgggta acgccagggt
      361 tttcccagtc acgacgttgt aaaacgacgg ccagtgaatt cgagctcggt acccggggat
      421 cctctagatt gaagttgatg cgatttgtga tggagaaacg gtcattattc caggaatcat
      481 ggaacatatt gaacgtgctg gtgtacatag tggtgactca atcgctgtat atccgccaca
      541 aactttgaca gaagacgagt tagcaacact tgaggactat actataaaat tagctaaagg
      601 tttaaacatc attggcttaa tcaacattca attcgttata gctcacgatg gtgtatatgt
      661 tttagaagta aacccacgtt ctagtagaac ggtaccattc ttaagtaaaa ttactgatat
      721 tccaatggca caattagcta tgcgagcaat cattggggaa aaactaacag atatgggtta
      781 tcaagaaggg gttcaaccat atgctgaggg tgtatttgtg aaagcaccgg tatttagttt
      841 taataaattg aaaaatgttg atattacttt aggacctgaa atgaagtcta caggtgaagt
      901 aatggggaaa gatactacat tagaaaaggc gttattcaaa gggttaacag gtagtggtgt
      961 tgaagttaaa gatcacggta cagtattaat gaccgtcagt gacaaagata aagaggaagt
     1021 tgttaaattg gcacaacgct taaatgaagt tggctataaa attttagcaa cgtctggaac
     1081 agctaataaa ttagctgagt atgacatacc tgcagaagta gtaggcaaaa tcggtggcga
     1141 aaatgattta ttaacacgta ttcaaaatgg tgatgttcaa atcgttataa atacaatgac
     1201 taaaggtaaa gaagtagaaa gggatggctt ccaaattaga cgtactacag ttgaaaatgg
     1261 tattccatgt ttgacatctt tagatacagc taatgcctta acgaatgtaa ttgaaagtat
     1321 gacatttaca atgcgtcaaa tgtaaatcaa tcaaactgta tcggtggggc tgtaattaac
     1381 catttactta aagaagttta tattacagcc tcattatttt aatgaatttc ttaatataaa
     1441 gggagacata tatgatgaaa gatttaccaa ttattgcatt agattttgaa tcaaaagaaa
     1501 aagtaaatca atttttagat ttatttgatg aatcattatt cgtaaaagta ggtatggaac
     1561 ttttttatca agaaggtcct caattaatta atgagataaa agaaagaggc catgatgtat
     1621 ttttagattt aaaactgcat gatattccta atacagttgg taaggcgatg gaaggactag
     1681 ctaaattgaa tgttgatctg gtaaatgttc atgctgctgg tggcgtaaaa atgatgtctg
     1741 aggccattaa aggattaaga aaacataatc aacatacaaa aattattgca gtaacacagc
     1801 ttacgtcaac aacagaagac atgttacgac acgaacaaaa tatacaaaca tcgattgaag
     1861 aggccgtttt aaattatgcc aagttagcaa atgcagctgg tttagatggc gttgtttgtt
     1921 cacctcttga aagtcgtatg ttgactgaaa agttaggtac atcattttta aaagtaacac
     1981 caggtattag acctaaaggt gcatctcaag atgaccaaca ccgtattacg acaccggaag
     2041 aagcaagaca gcttggttcg acgcatattg tagtcggtag accgattaca caaagtgaca
     2101 atccagtcga aagttatcat aaaattaaag aaagttggtt agtataatgg ctaaagaaat
     2161 tgcaaaatca ttattagata ttgaagctgt aacattatca ccaaatgatt tatatacatg
     2221 gagttcaggt attaaatcac cgatttactg tgataaccgt gttacgttag gttatccttt
     2281 agttcgaggc gcaatccgcg atggtttaat taacttaatt aaagaacact ttcctgaagt
     2341 agaagttatt tctggtactg caacagctgg tattccacat gcagctttta ttgctgaaaa
     2401 attaaaatta ccaatgaatt atgttcgttc atcaaataag agtcatggta agcaaaatca
     2461 aatcgaaggt gctaaaagtg aaggtaaaaa agtagttgtg atagaagatt taatttcgac
     2521 agggggatct tcagtcacag cagttgaagc cttaaaacaa gcaggtgcag aagtattagg
     2581 tgttgtagct atctttactt acggtttgaa aaaagcagat gatacattta gcaatattca
     2641 actacctttt tacactttaa gtgattacaa tgaattaatt gaagtagctg aaaatgaagg
     2701 taaaatttct agtgaagata tccaaacatt agttgaatgg agagacaact tagcataata
     2761 tagacactag aaggaggaat tcaacaaatg aatgacaaaa catctaatga tttatatggg
     2821 aagataaaac attgtaacga atttatcaat cattcaaatg attccaatct atctagtagt
     2881 cacgatgtcg acgaaagttc aacgaagcaa aaacatataa aaaataaaac aactatagat
     2941 cataatgatg atttatttaa acatgtaaag gatatattac gtaaacaagg acaaatttaa
     3001 aaacaaaaaa gctatagtgt ttaatatcag gggctgctta accgcatccg aatattacaa
     3061 acactatagc ttttattata taaaaaattt attagcggat aattaacttt attaaaatcc
     3121 aaccaattaa gaatacgagt aataggacta atactggaat cacataatgt aacataacgt
     3181 ccctccttta acttaatttt aattgtaatc aaatttgaca ataagtcaaa acattaatac
     3241 ctatgataag tatcatttat taacatatgt atcatatttt taatcttgcg taatttttat
     3301 cgttaactat gtgatttaat caacaggcca ataagacgtt tgtcattcgt gcacataacg
     3361 gtatgacgcg cttttgtcat tttaattatg ttaagataat agtagttata gaagttcgat
     3421 ctataggagg catagcatgg atattccaaa aatcacgaca tttttaatgt ttaataacaa
     3481 agctgaagaa gctgttaaac tatacacaag cttatttgaa gatagtgaga ttataacaat
     3541 ggctaagtat ggtgaaaatg gacctggtga tcacgggact gtacaacact caatatttac
     3601 attaaatgga caagtattca tggcgattga tgctaatagt ggcacagaat taccaatgaa
     3661 tcctgcgatt tcattatttg ttacagtaaa agatactatt gaaatggaac gactatttaa
     3721 tggattaaaa gatgaaggtg ccattttaat gccaaaaacg aatatgccac catacagaga
     3781 gtttgctaga tcttacgtgt gctctgcgag gcttaaacct gtttttgcta acgcaaaaaa
     3841 tgagtggcaa aatgctagcc actcatagtt ctaaaccaaa atataatata actattcaaa
     3901 ctgcttttca gaacgtttaa atacaataat cgtcaaaaga caacataaaa taataaggat
     3961 agccatggct acaagaatat tactatacac tccagaagaa taatttataa attccagaac
     4021 tagtttacga ttaatcaatt gtagtgacaa taaacctcct acaattgcta tacctgttcc
     4081 ctctgataaa aaacttgtga aatttagcaa actcattcca gaagcaactt cttcttcaga
     4141 aagactactt gatactattt ttgatataac tgttttagta aaagataatc cgcccataac
     4201 aaatataaac ataaaagtag tcaaccacat actaaactca acaaaaaatg caatagttaa
     4261 aaaacttata gagatagaca atgatcctaa aataaaaaca aataatgatc cttttctatc
     4321 cactaaaaaa ccaccaaaat aaccaaaaac aataacactc atggttccag gaaaaataac
     4381 actattacct attgtcgcta catttacatg ataaatagtt ttcatcatat aaggcaccat
     4441 tgatataaaa ccagctacta tagaaaatat tagcccacca gaaaacaaac caagcataaa
     4501 cggaatgttt ttccctagtt taggattaat aaaagggtta gaaactcttg aaatatgttt
     4561 aataaaaatc acaaaaaaga ttgtgaagag tattaaaaaa gtccaattat aatttgtcgt
     4621 aaataacata aaacatataa tacttataga cattaaaaca atacctacga tatctaatgt
     4681 attttttgtt gatttaccag gtaccattac tttaataaga aaaggtatag ttactattgt
     4741 aatcatagga agtataagta ggtaagacca atgaatataa tgtgctatta ttccccctat
     4801 tgaaggacct aacccttcac ctaaagctac aattgatcct ataaaaccaa aggctttgcc
     4861 ttgttttttt cttgtaatat ttctagctac aaccaccata atcagtgaag ggaatgcagc
     4921 agatcctact ccttgtacta acctaccaaa aatcaaaata aaaaagtgat tgtgaccaat
     4981 aaaagcaatc aatgaaccaa gacagctcaa actaatacca ataattaaca atttttttat
     5041 atttatataa tcagataatt ttccatatac tgctgttcct atcgaaaaag ttaacatata
     5101 tgcagtgttt acccagtttg taattccagg agtagtatta aaatgatttg caatatcagg
     5161 taaagaaaca tttaaaacca tttcatttaa tacactaaaa aatgaaagaa tacaaagcca
     5221 aaataaaacg ctataaaaca aacctttaaa ttttttatat aaactaaaca attttacctc
     5281 tttacttgag gtgactaaag tttatagggg taaattattc tttcacctca tataaattcc
     5341 ccaaattttt aaatagacac ttcataaaaa aatcctccta aattagaata gtttaaatat
     5401 atcatttttg ttcagtaata ttaatatgta cactatttcc aaaatttaaa ttcatgttgc
     5461 caaaaatcga tttgtttttg caattgtttt tcgttcgctt ttaaagtcga tttcattaag
     5521 tccgttaaat cgactggcga aatttctttt atcaaattct tatattggat ctgctgcatt
     5581 aatgaatcgg ccaacgcgcg gggagaggcg gtttgcgtat tgggcgctct tccgcttcct
     5641 cgctcactga ctcgctgcgc tcggtcgttc ggctgcggcg cgccgtcggc ttaaaccagt
     5701 tttcgttggt gcgaaaaaag agtgtcttgt gacactctta aattcaaaat ctatcggtca
     5761 gatttatacc gatttgattt tatatattct tgaataacat acgccgagtt atcacataaa
     5821 agcgggaacc aatcatcaaa tttaaacttc attgcataat ccattaaact cttaaattct
     5881 acgattcctt gttcatcaat aaactcaatc atttctttaa ttaatttata tctatctgtt
     5941 gttgttttct ttaataattc atcaacatct acaccgccat aaactatcat atcttctttt
     6001 tgatatttaa atttattagg atcgtccatg tgaagcatat atctcacaag acctttcaca
     6061 cttcctgcaa tctgcggaat agtcgcattc aattcttctg taattatttt tatctgttca
     6121 taagatttat taccctcata catcactaga atatgataat gctctttttt catcctatct
     6181 tctgtatcag tatccctatc atgtaatgga gacactacaa attgaatgtg taactctttt
     6241 aaatactcta accactcggc ttttgctgat tctggatata aaacaaatgt ccaattacgt
     6301 cctcttgaat ttttcttgtt ttcagtttct tttattacat tttcgctcat gatataataa
     6361 cggtgctaat acatttaaca aaatttagtc atagataggc agcatgccag tgctgtctat
     6421 ctttttttgt ttaaaatgca ccgtattcct cctttgcata tttttttatt agaataccgg
     6481 ttgcatctga tttgctaata ttatattttt ctttgattct atttaatatc tcattttctt
     6541 ctgttgtaag tcttaaagta acagcaactt ttttctcttc ttttctatct acaaccatca
     6601 ctgtacctcc caacatctgt ttttttcact ttaacataaa aaacaacctt ttaacattaa
     6661 aaacccaata tttatttatt tgtttggaca atggacaatg gacacctagg ggggaggtcg
     6721 tagtaccccc ctatgttttc tcccctaaat aaccccaaaa atctaagaaa aaaagacctc
     6781 aaaaaggtct ttaattaaca tctcaaattt cgcatttatt ccaatttcct ttttgcgtgt
     6841 ggcgcgccgg cacgcgtatc gatgctcact caaaggcggt aatacggtta tccacagaat
     6901 caggggataa cgcaggaaag aacatgtgag caaaaggcca gcaaaaggcc aggaaccgta
     6961 aaaaggccgc gttgctggcg tttttccata ggctccgccc ccctgacgag catcacaaaa
     7021 atcgacgctc aagtcagagg tggcgaaacc cgacaggact ataaagatac caggcgtttc
     7081 cccctggaag ctccctcgtg cgctctcctg ttccgaccct gccgcttacc ggatacctgt
     7141 ccgcctttct cccttcggga agcgtggcgc tttctcatag ctcacgctgt aggtatctca
     7201 gttcggtgta ggtcgttcgc tccaagctgg gctgtgtgca cgaacccccc gttcagcccg
     7261 accgctgcgc cttatccggt aactatcgtc ttgagtccaa cccggtaaga cacgacttat
     7321 cgccactggc agcagccact ggtaacagga ttagcagagc gaggtatgta ggcggtgcta
     7381 cagagttctt gaagtggtgg cctaactacg gctacactag aaggacagta tttggtatct
     7441 gcgctctgct gaagccagtt accttcggaa aaagagttgg tagctcttga tccggcaaac
     7501 aaaccaccgc tggtagcggt ggtttttttg tttgcaagca gcagattacg cgcagaaaaa
     7561 aaggatctca agaagatcct ttgatctttt ctacggggtc tgacgctcag tggaacgaaa
     7621 actcacgtta agggattttg gtcatgagat tatcaaaaag gatcttcacc tagatccttt
     7681 taaattaaaa atgaagtttt aaatcaatct aaagtatata tgagtaaact tggtctgaca
     7741 gttaccaatg cttaatcagt gaggcaccta tctcagcgat ctgtctattt cgttcatcca
     7801 tagttgcctg actccccgtc gtgtagataa ctacgatacg ggagggctta ccatctggcc
     7861 ccagtgctgc aatgataccg cgagacccac gctcaccggc tccagattta tcagcaataa
     7921 accagccagc cggaagggcc gagcgcagaa gtggtcctgc aactttatcc gcctccatcc
     7981 agtctattaa ttgttgccgg gaagctagag taagtagttc gccagttaat agtttgcgca
     8041 acgttgttgc cattgctaca ggcatcgtgg tgtcacgctc gtcgtttggt atggcttcat
     8101 tcagctccgg ttcccaacga tcaaggcgag ttacatgatc ccccatgttg tgcaaaaaag
     8161 cggttagctc cttcggtcct ccgatcgttg tcagaagtaa gttggccgca gtgttatcac
     8221 tcatggttat ggcagcactg cataattctc ttactgtcat gccatccgta agatgctttt
     8281 ctgtgactgg tgagtactca accaagtcat tctgagaata gtgtatgcgg cgaccgagtt
     8341 gctcttgccc ggcgtcaata cgggataata ccgcgccaca tagcagaact ttaaaagtgc
     8401 tcatcattgg aaaacgttct tcggggcgaa aactctcaag gatcttaccg ctgttgagat
     8461 ccagttcgat gtaacccact cgtgcaccca actgatcttc agcatctttt actttcacca
     8521 gcgtttctgg gtgagcaaaa acaggaaggc aaaatgccgc aaaaaaggga ataagggcga
     8581 cacggaaatg ttgaatactc atactcttcc tttttcaata ttattgaagc atttatcagg
     8641 gttattgtct catgagcgga tacatatttg aatgtattta gaaaaataaa caaatagggg
     8701 ttccgcgcac atttccccga aaagtgccac ctgacgtcta agaaaccatt attatcatga
     8761 cattaaccta taaaaatagg cgtatcacga ggccctttcg tc
//
