LOCUS       V003695                 8108 bp    DNA     circular SYN 18-DEC-2018
DEFINITION  Exported.
ACCESSION   V003695
VERSION     V003695
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
            .
REFERENCE   1  (bases 1 to 8108)
  AUTHORS   Redder P, Linder P.
  TITLE     New Range of Vectors with a Stringent 5-Fluoroorotic Acid-Based
            Counterselection System for Generating Mutants by Allelic
            Replacement in Staphylococcus aureus
  JOURNAL   Appl. Environ. Microbiol. 78 (11), 3846-3854 (2012)
   PUBMED   22447609
REFERENCE   2  (bases 1 to 8108)
  AUTHORS   Redder P, Linder P.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2012) Department of Microbiology and Molecular
            Medicine, University of Geneva, Faculty of Medicine, Rue
            Michel-Servet 1, Geneva 1203, Switzerland
REFERENCE   3  (bases 1 to 8108)
  TITLE     Direct Submission
REFERENCE   4  (bases 1 to 8108)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     ##Assembly-Data-START##
            Assembly Method       :: BioEdit, CAP v. 7.0.9.0
            Sequencing Technology :: Sanger dideoxy sequencing
            ##Assembly-Data-END##
            SGRef: number: 1; type: "Journal Article"; journalName: "Appl.
            Environ. Microbiol."; date: "2012"; volume: "78"; issue: "11";
            pages: "3846-3854"
            SGRef: number: 2; type: "Journal Article"; journalName: "Submitted
            (12-MAR-2012) Department of Microbiology and Molecular Medicine,
            University of Geneva, Faculty of Medicine, Rue Michel-Servet 1,
            Geneva 1203, Switzerland"
            SGRef: number: 3; type: "Journal Article"
FEATURES             Location/Qualifiers
     source          1..8108
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     379..395
                     /label="M13 fwd"
                     /note="common sequencing primer, one of multiple similar
                     variants"
     CDS             1455..2144
                     /gene="pyrF"
                     /label="Orotidine 5'-phosphate decarboxylase"
                     /note="Orotidine 5'-phosphate decarboxylase from
                     Staphylococcus aureus (strain MSSA476). Accession#: Q6GA09"
     CDS             complement(3855..4586)
                     /gene="ermC"
                     /label="rRNA adenine N-6-methyltransferase"
                     /note="rRNA adenine N-6-methyltransferase from
                     Staphylococcus aureus. Accession#: P02979"
     rep_origin      complement(4985..6148)
                     /direction=LEFT
                     /note="thermosensitive origin of replication; for
                     replication in Staphylococcus aureus; pE194ts"
     rep_origin      complement(6289..6877)
                     /direction=LEFT
                     /label="ori"
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
                     replication"
     CDS             complement(7051..7908)
                     /label="AmpR"
                     /note="beta-lactamase"
     promoter        complement(7909..8013)
                     /label="AmpR promoter"
ORIGIN
        1 tcgcgcgttt cggtgatgac ggtgaaaacc tctgacacat gcagctcccg gagacggtca
       61 cagcttgtct gtaagcggat gccgggagca gacaagcccg tcagggcgcg tcagcgggtg
      121 ttggcgggtg tcggggctgg cttaactatg cggcatcaga gcagattgta ctgagagtgc
      181 accatatgcg gtgtgaaata ccgcacagat gcgtaaggag aaaataccgc atcaggcgcc
      241 attcgccatt caggctgcgc aactgttggg aagggcgatc ggtgcgggcc tcttcgctat
      301 tacgccagct gtcgaatggg ggatgtgctg caaggcgatt aagttgggta acgccagggt
      361 tttcccagtc acgacgttgt aaaacgacgg ccagtgaatt cgagctcggt acccggggat
      421 cctctagatt gaagttgatg cgatttgtga tggagaaacg gtcattattc caggaatcat
      481 ggaacatatt gaacgtgctg gtgtacatag tggtgactca atcgctgtat atccgccaca
      541 aactttgaca gaagacgagt tagcaacact tgaggactat actataaaat tagctaaagg
      601 tttaaacatc attggcttaa tcaacattca attcgttata gctcacgatg gtgtatatgt
      661 tttagaagta aacccacgtt ctagtagaac ggtaccattc ttaagtaaaa ttactgatat
      721 tccaatggca caattagcta tgcgagcaat cattggggaa aaactaacag atatgggtta
      781 tcaagaaggg gttcaaccat atgctgaggg tgtatttgtg aaagcaccgg tatttagttt
      841 taataaattg aaaaatgttg atattacttt aggacctgaa atgaagtcta caggtgaagt
      901 aatggggaaa gatactacat tagaaaaggc gttattcaaa gggttaacag gtagtggtgt
      961 tgaagttaaa gatcacggta cagtattaat gaccgtcagt gacaaagata aagaggaagt
     1021 tgttaaattg gcacaacgct taaatgaagt tggctataaa attttagcaa cgtctggaac
     1081 agctaataaa ttagctgagt atgacatacc tgcagaagta gtaggcaaaa tcggtggcga
     1141 aaatgattta ttaacacgta ttcaaaatgg tgatgttcaa atcgttataa atacaatgac
     1201 taaaggtaaa gaagtagaaa gggatggctt ccaaattaga cgtactacag ttgaaaatgg
     1261 tattccatgt ttgacatctt tagatacagc taatgcctta acgaatgtaa ttgaaagtat
     1321 gacatttaca atgcgtcaaa tgtaaatcaa tcaaactgta tcggtggggc tgtaattaac
     1381 catttactta aagaagttta tattacagcc tcattatttt aatgaatttc ttaatataaa
     1441 gggagacata tatgatgaaa gatttaccaa ttattgcatt agattttgaa tcaaaagaaa
     1501 aagtaaatca atttttagat ttatttgatg aatcattatt cgtaaaagta ggtatggaac
     1561 ttttttatca agaaggtcct caattaatta atgagataaa agaaagaggc catgatgtat
     1621 ttttagattt aaaactgcat gatattccta atacagttgg taaggcgatg gaaggactag
     1681 ctaaattgaa tgttgatctg gtaaatgttc atgctgctgg tggcgtaaaa atgatgtctg
     1741 aggccattaa aggattaaga aaacataatc aacatacaaa aattattgca gtaacacagc
     1801 ttacgtcaac aacagaagac atgttacgac acgaacaaaa tatacaaaca tcgattgaag
     1861 aggccgtttt aaattatgcc aagttagcaa atgcagctgg tttagatggc gttgtttgtt
     1921 cacctcttga aagtcgtatg ttgactgaaa agttaggtac atcattttta aaagtaacac
     1981 caggtattag acctaaaggt gcatctcaag atgaccaaca ccgtattacg acaccggaag
     2041 aagcaagaca gcttggttcg acgcatattg tagtcggtag accgattaca caaagtgaca
     2101 atccagtcga aagttatcat aaaattaaag aaagttggtt agtataatgg ctaaagaaat
     2161 tgcaaaatca ttattagata ttgaagctgt aacattatca ccaaatgatt tatatacatg
     2221 gagttcaggt attaaatcac cgatttactg tgataaccgt gttacgttag gttatccttt
     2281 agttcgaggc gcaatccgcg atggtttaat taacttaatt aaagaacact ttcctgaagt
     2341 agaagttatt tctggtactg caacagctgg tattccacat gcagctttta ttgctgaaaa
     2401 attaaaatta ccaatgaatt atgttcgttc atcaaataag agtcatggta agcaaaatca
     2461 aatcgaaggt gctaaaagtg aaggtaaaaa agtagttgtg atagaagatt taatttcgac
     2521 agggggatct tcagtcacag cagttgaagc cttaaaacaa gcaggtgcag aagtattagg
     2581 tgttgtagct atctttactt acggtttgaa aaaagcagat gatacattta gcaatattca
     2641 actacctttt tacactttaa gtgattacaa tgaattaatt gaagtagctg aaaatgaagg
     2701 taaaatttct agtgaagata tccaaacatt agttgaatgg agagacaact tagcataata
     2761 tagacactag aaggaggaat tcaacaaatg aatgacaaaa catctaatga tttatatggg
     2821 aagataaaac attgtaacga atttatcaat cattcaaatg attccaatct atctagtagt
     2881 cacgatgtcg acgaaagttc aacgaagcaa aaacatataa aaaataaaac aactatagat
     2941 cataatgatg atttatttaa acatgtaaag gatatattac gtaaacaagg acaaatttaa
     3001 aaacaaaaaa gctatagtgt ttaatatcag gggctgctta accgcatccg aatattacaa
     3061 acactatagc ttttattata taaaaaattt attagcggat aattaacttt attaaaatcc
     3121 aaccaattaa gaatacgagt aataggacta atactggaat cacataatgt aacataacgt
     3181 ccctccttta acttaatttt aattgtaatc aaatttgaca ataagtcaaa acattaatac
     3241 ctatgataag tatcatttat taacatatgt atcatatttt taatcttgcg taatttttat
     3301 cgttaactat gtgatttaat caacaggcca ataagacgtt tgtcattcgt gcacataacg
     3361 gtatgacgcg cttttgtcat tttaattatg ttaagataat agtagttata gaagttcgat
     3421 ctataggagg catagcatgg atattccaaa aatcacgaca tttttaatgt ttaataacaa
     3481 agctgaagaa gctgttaaac tatacacaag cttatttgaa gatagtgaga ttataacaat
     3541 ggctaagtat ggtgaaaatg gacctggtga tcacgggact gtacaacact caatatttac
     3601 attaaatgga caagtattca tggcgattga tgctaatagt ggcacagaat taccaatgaa
     3661 tcctgcgatt tcattatttg ttacagtaaa agatactatt gaaatggaac gactatttaa
     3721 tggattaaaa gatgaaggtg ccattttaat gccaaaaacg aatatgccac catacagaga
     3781 gtttgctaga tctcacaaaa aataggcaca cgaaaaacaa gttaagggat gcagtttatg
     3841 catcccttaa cttacttatt aaataattta tagctattga aaagagataa gaattgttca
     3901 aagctaatat tgtttaaatc gtcaattcct gcatgtttta aggaattgtt aaattgattt
     3961 tttgtaaata ttttcttgta ttctttgtta acccatttca taacgaaata attatacttt
     4021 tgtttatctt tgtgtgatat tcttgatttt ttcctactta atctgataag tgagctattc
     4081 actttaggtt taggatgaaa atattctctt ggaaccatac ttaatataga aatatcaact
     4141 tctgccatta aaagtaatgc caatgagcgt tttgtattta ataatctttt agcaaacccg
     4201 tattccacga ttaaataaat ctcattagct atactatcaa aaacaatttt gcgtattata
     4261 tccgtactta tgttataagg tatattacca tatattttat aggattggtt tttaggaaat
     4321 ttaaactgca atatatcctt gtttaaaact tggaaattat cgtgatcaac aagtttattt
     4381 tctgtagttt tgcataattt atggtctatt tcaatggcag ttacgaaatt acacctcttt
     4441 actaattcaa gggtaaaatg gccttttcct gagccgattt caaagatatt atcatgttca
     4501 tttaatctta tatttgtcat tattttatct atattatgtt ttgaagtaat aaagttttga
     4561 ctgtgtttta tatttttctc gttcattata accctcttta atttggttat atgaattttg
     4621 cttattaacg attcattata accacttatt ttttgtttgg ttgataatga actgtgctga
     4681 ttacaaaaat actaaaaatg cccatatttt ttcctcctta taaaattagt ataattatag
     4741 cacaagctct gataaatatg aacatgatga gtgatcgtta aatttatact gcaatcggat
     4801 gcgattattg aataaaagat atgagagatt tatctaattt cttttttctt gtaaaaaaag
     4861 aaagttctta aaggatctgc tgcattaatg aatcggccaa cgcgcgggga gaggcggttt
     4921 gcgtattggg cgctcttccg cttcctcgct cactgactcg ctgcgctcgg tcgttcggct
     4981 gcggcgcgcc gtcggcttaa accagttttc gttggtgcga aaaaagagtg tcttgtgaca
     5041 ctcttaaatt caaaatctat cggtcagatt tataccgatt tgattttata tattcttgaa
     5101 taacatacgc cgagttatca cataaaagcg ggaaccaatc atcaaattta aacttcattg
     5161 cataatccat taaactctta aattctacga ttccttgttc atcaataaac tcaatcattt
     5221 ctttaattaa tttatatcta tctgttgttg ttttctttaa taattcatca acatctacac
     5281 cgccataaac tatcatatct tctttttgat atttaaattt attaggatcg tccatgtgaa
     5341 gcatatatct cacaagacct ttcacacttc ctgcaatctg cggaatagtc gcattcaatt
     5401 cttctgtaat tatttttatc tgttcataag atttattacc ctcatacatc actagaatat
     5461 gataatgctc ttttttcatc ctatcttctg tatcagtatc cctatcatgt aatggagaca
     5521 ctacaaattg aatgtgtaac tcttttaaat actctaacca ctcggctttt gctgattctg
     5581 gatataaaac aaatgtccaa ttacgtcctc ttgaattttt cttgttttca gtttctttta
     5641 ttacattttc gctcatgata taataacggt gctaatacat ttaacaaaat ttagtcatag
     5701 ataggcagca tgccagtgct gtctatcttt ttttgtttaa aatgcaccgt attcctcctt
     5761 tgcatatttt tttattagaa taccggttgc atctgatttg ctaatattat atttttcttt
     5821 gattctattt aatatctcat tttcttctgt tgtaagtctt aaagtaacag caactttttt
     5881 ctcttctttt ctatctacaa ccatcactgt acctcccaac atctgttttt ttcactttaa
     5941 cataaaaaac aaccttttaa cattaaaaac ccaatattta tttatttgtt tggacaatgg
     6001 acaatggaca cctagggggg aggtcgtagt acccccctat gttttctccc ctaaataacc
     6061 ccaaaaatct aagaaaaaaa gacctcaaaa aggtctttaa ttaacatctc aaatttcgca
     6121 tttattccaa tttccttttt gcgtgtggcg cgccggcacg cgtatcgatg ctcactcaaa
     6181 ggcggtaata cggttatcca cagaatcagg ggataacgca ggaaagaaca tgtgagcaaa
     6241 aggccagcaa aaggccagga accgtaaaaa ggccgcgttg ctggcgtttt tccataggct
     6301 ccgcccccct gacgagcatc acaaaaatcg acgctcaagt cagaggtggc gaaacccgac
     6361 aggactataa agataccagg cgtttccccc tggaagctcc ctcgtgcgct ctcctgttcc
     6421 gaccctgccg cttaccggat acctgtccgc ctttctccct tcgggaagcg tggcgctttc
     6481 tcatagctca cgctgtaggt atctcagttc ggtgtaggtc gttcgctcca agctgggctg
     6541 tgtgcacgaa ccccccgttc agcccgaccg ctgcgcctta tccggtaact atcgtcttga
     6601 gtccaacccg gtaagacacg acttatcgcc actggcagca gccactggta acaggattag
     6661 cagagcgagg tatgtaggcg gtgctacaga gttcttgaag tggtggccta actacggcta
     6721 cactagaagg acagtatttg gtatctgcgc tctgctgaag ccagttacct tcggaaaaag
     6781 agttggtagc tcttgatccg gcaaacaaac caccgctggt agcggtggtt tttttgtttg
     6841 caagcagcag attacgcgca gaaaaaaagg atctcaagaa gatcctttga tcttttctac
     6901 ggggtctgac gctcagtgga acgaaaactc acgttaaggg attttggtca tgagattatc
     6961 aaaaaggatc ttcacctaga tccttttaaa ttaaaaatga agttttaaat caatctaaag
     7021 tatatatgag taaacttggt ctgacagtta ccaatgctta atcagtgagg cacctatctc
     7081 agcgatctgt ctatttcgtt catccatagt tgcctgactc cccgtcgtgt agataactac
     7141 gatacgggag ggcttaccat ctggccccag tgctgcaatg ataccgcgag acccacgctc
     7201 accggctcca gatttatcag caataaacca gccagccgga agggccgagc gcagaagtgg
     7261 tcctgcaact ttatccgcct ccatccagtc tattaattgt tgccgggaag ctagagtaag
     7321 tagttcgcca gttaatagtt tgcgcaacgt tgttgccatt gctacaggca tcgtggtgtc
     7381 acgctcgtcg tttggtatgg cttcattcag ctccggttcc caacgatcaa ggcgagttac
     7441 atgatccccc atgttgtgca aaaaagcggt tagctccttc ggtcctccga tcgttgtcag
     7501 aagtaagttg gccgcagtgt tatcactcat ggttatggca gcactgcata attctcttac
     7561 tgtcatgcca tccgtaagat gcttttctgt gactggtgag tactcaacca agtcattctg
     7621 agaatagtgt atgcggcgac cgagttgctc ttgcccggcg tcaatacggg ataataccgc
     7681 gccacatagc agaactttaa aagtgctcat cattggaaaa cgttcttcgg ggcgaaaact
     7741 ctcaaggatc ttaccgctgt tgagatccag ttcgatgtaa cccactcgtg cacccaactg
     7801 atcttcagca tcttttactt tcaccagcgt ttctgggtga gcaaaaacag gaaggcaaaa
     7861 tgccgcaaaa aagggaataa gggcgacacg gaaatgttga atactcatac tcttcctttt
     7921 tcaatattat tgaagcattt atcagggtta ttgtctcatg agcggataca tatttgaatg
     7981 tatttagaaa aataaacaaa taggggttcc gcgcacattt ccccgaaaag tgccacctga
     8041 cgtctaagaa accattatta tcatgacatt aacctataaa aataggcgta tcacgaggcc
     8101 ctttcgtc
//
