LOCUS       V003696                 8026 bp    DNA     circular SYN 18-DEC-2018
DEFINITION  Exported.
ACCESSION   V003696
VERSION     V003696
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
            .
REFERENCE   1  (bases 1 to 8026)
  AUTHORS   Redder P, Linder P.
  TITLE     New Range of Vectors with a Stringent 5-Fluoroorotic Acid-Based
            Counterselection System for Generating Mutants by Allelic
            Replacement in Staphylococcus aureus
  JOURNAL   Appl. Environ. Microbiol. 78 (11), 3846-3854 (2012)
   PUBMED   22447609
REFERENCE   2  (bases 1 to 8026)
  AUTHORS   Redder P, Linder P.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2012) Department of Microbiology and Molecular
            Medicine, University of Geneva, Faculty of Medicine, Rue
            Michel-Servet 1, Geneva 1203, Switzerland
REFERENCE   3  (bases 1 to 8026)
  TITLE     Direct Submission
REFERENCE   4  (bases 1 to 8026)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     ##Assembly-Data-START##
            Assembly Method       :: BioEdit, CAP v. 7.0.9.0
            Sequencing Technology :: Sanger dideoxy sequencing
            ##Assembly-Data-END##
            SGRef: number: 1; type: "Journal Article"; journalName: "Appl.
            Environ. Microbiol."; date: "2012"; volume: "78"; issue: "11";
            pages: "3846-3854"
            SGRef: number: 2; type: "Journal Article"; journalName: "Submitted
            (12-MAR-2012) Department of Microbiology and Molecular Medicine,
            University of Geneva, Faculty of Medicine, Rue Michel-Servet 1,
            Geneva 1203, Switzerland"
            SGRef: number: 3; type: "Journal Article"
FEATURES             Location/Qualifiers
     source          1..8026
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     379..395
                     /label="M13 fwd"
                     /note="common sequencing primer, one of multiple similar
                     variants"
     CDS             1455..2144
                     /gene="pyrF"
                     /label="Orotidine 5'-phosphate decarboxylase"
                     /note="Orotidine 5'-phosphate decarboxylase from
                     Staphylococcus aureus (strain MSSA476). Accession#: Q6GA09"
     CDS             complement(3869..4516)
                     /gene="cat"
                     /label="Chloramphenicol acetyltransferase"
                     /note="Chloramphenicol acetyltransferase from
                     Staphylococcus aureus. Accession#: P00485"
     rep_origin      complement(4903..6066)
                     /direction=LEFT
                     /note="thermosensitive origin of replication; for
                     replication in Staphylococcus aureus; pE194ts"
     rep_origin      complement(6207..6795)
                     /direction=LEFT
                     /label="ori"
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
                     replication"
     CDS             complement(6969..7826)
                     /label="AmpR"
                     /note="beta-lactamase"
     promoter        complement(7827..7931)
                     /label="AmpR promoter"
ORIGIN
        1 tcgcgcgttt cggtgatgac ggtgaaaacc tctgacacat gcagctcccg gagacggtca
       61 cagcttgtct gtaagcggat gccgggagca gacaagcccg tcagggcgcg tcagcgggtg
      121 ttggcgggtg tcggggctgg cttaactatg cggcatcaga gcagattgta ctgagagtgc
      181 accatatgcg gtgtgaaata ccgcacagat gcgtaaggag aaaataccgc atcaggcgcc
      241 attcgccatt caggctgcgc aactgttggg aagggcgatc ggtgcgggcc tcttcgctat
      301 tacgccagct gtcgaatggg ggatgtgctg caaggcgatt aagttgggta acgccagggt
      361 tttcccagtc acgacgttgt aaaacgacgg ccagtgaatt cgagctcggt acccggggat
      421 cctctagatt gaagttgatg cgatttgtga tggagaaacg gtcattattc caggaatcat
      481 ggaacatatt gaacgtgctg gtgtacatag tggtgactca atcgctgtat atccgccaca
      541 aactttgaca gaagacgagt tagcaacact tgaggactat actataaaat tagctaaagg
      601 tttaaacatc attggcttaa tcaacattca attcgttata gctcacgatg gtgtatatgt
      661 tttagaagta aacccacgtt ctagtagaac ggtaccattc ttaagtaaaa ttactgatat
      721 tccaatggca caattagcta tgcgagcaat cattggggaa aaactaacag atatgggtta
      781 tcaagaaggg gttcaaccat atgctgaggg tgtatttgtg aaagcaccgg tatttagttt
      841 taataaattg aaaaatgttg atattacttt aggacctgaa atgaagtcta caggtgaagt
      901 aatggggaaa gatactacat tagaaaaggc gttattcaaa gggttaacag gtagtggtgt
      961 tgaagttaaa gatcacggta cagtattaat gaccgtcagt gacaaagata aagaggaagt
     1021 tgttaaattg gcacaacgct taaatgaagt tggctataaa attttagcaa cgtctggaac
     1081 agctaataaa ttagctgagt atgacatacc tgcagaagta gtaggcaaaa tcggtggcga
     1141 aaatgattta ttaacacgta ttcaaaatgg tgatgttcaa atcgttataa atacaatgac
     1201 taaaggtaaa gaagtagaaa gggatggctt ccaaattaga cgtactacag ttgaaaatgg
     1261 tattccatgt ttgacatctt tagatacagc taatgcctta acgaatgtaa ttgaaagtat
     1321 gacatttaca atgcgtcaaa tgtaaatcaa tcaaactgta tcggtggggc tgtaattaac
     1381 catttactta aagaagttta tattacagcc tcattatttt aatgaatttc ttaatataaa
     1441 gggagacata tatgatgaaa gatttaccaa ttattgcatt agattttgaa tcaaaagaaa
     1501 aagtaaatca atttttagat ttatttgatg aatcattatt cgtaaaagta ggtatggaac
     1561 ttttttatca agaaggtcct caattaatta atgagataaa agaaagaggc catgatgtat
     1621 ttttagattt aaaactgcat gatattccta atacagttgg taaggcgatg gaaggactag
     1681 ctaaattgaa tgttgatctg gtaaatgttc atgctgctgg tggcgtaaaa atgatgtctg
     1741 aggccattaa aggattaaga aaacataatc aacatacaaa aattattgca gtaacacagc
     1801 ttacgtcaac aacagaagac atgttacgac acgaacaaaa tatacaaaca tcgattgaag
     1861 aggccgtttt aaattatgcc aagttagcaa atgcagctgg tttagatggc gttgtttgtt
     1921 cacctcttga aagtcgtatg ttgactgaaa agttaggtac atcattttta aaagtaacac
     1981 caggtattag acctaaaggt gcatctcaag atgaccaaca ccgtattacg acaccggaag
     2041 aagcaagaca gcttggttcg acgcatattg tagtcggtag accgattaca caaagtgaca
     2101 atccagtcga aagttatcat aaaattaaag aaagttggtt agtataatgg ctaaagaaat
     2161 tgcaaaatca ttattagata ttgaagctgt aacattatca ccaaatgatt tatatacatg
     2221 gagttcaggt attaaatcac cgatttactg tgataaccgt gttacgttag gttatccttt
     2281 agttcgaggc gcaatccgcg atggtttaat taacttaatt aaagaacact ttcctgaagt
     2341 agaagttatt tctggtactg caacagctgg tattccacat gcagctttta ttgctgaaaa
     2401 attaaaatta ccaatgaatt atgttcgttc atcaaataag agtcatggta agcaaaatca
     2461 aatcgaaggt gctaaaagtg aaggtaaaaa agtagttgtg atagaagatt taatttcgac
     2521 agggggatct tcagtcacag cagttgaagc cttaaaacaa gcaggtgcag aagtattagg
     2581 tgttgtagct atctttactt acggtttgaa aaaagcagat gatacattta gcaatattca
     2641 actacctttt tacactttaa gtgattacaa tgaattaatt gaagtagctg aaaatgaagg
     2701 taaaatttct agtgaagata tccaaacatt agttgaatgg agagacaact tagcataata
     2761 tagacactag aaggaggaat tcaacaaatg aatgacaaaa catctaatga tttatatggg
     2821 aagataaaac attgtaacga atttatcaat cattcaaatg attccaatct atctagtagt
     2881 cacgatgtcg acgaaagttc aacgaagcaa aaacatataa aaaataaaac aactatagat
     2941 cataatgatg atttatttaa acatgtaaag gatatattac gtaaacaagg acaaatttaa
     3001 aaacaaaaaa gctatagtgt ttaatatcag gggctgctta accgcatccg aatattacaa
     3061 acactatagc ttttattata taaaaaattt attagcggat aattaacttt attaaaatcc
     3121 aaccaattaa gaatacgagt aataggacta atactggaat cacataatgt aacataacgt
     3181 ccctccttta acttaatttt aattgtaatc aaatttgaca ataagtcaaa acattaatac
     3241 ctatgataag tatcatttat taacatatgt atcatatttt taatcttgcg taatttttat
     3301 cgttaactat gtgatttaat caacaggcca ataagacgtt tgtcattcgt gcacataacg
     3361 gtatgacgcg cttttgtcat tttaattatg ttaagataat agtagttata gaagttcgat
     3421 ctataggagg catagcatgg atattccaaa aatcacgaca tttttaatgt ttaataacaa
     3481 agctgaagaa gctgttaaac tatacacaag cttatttgaa gatagtgaga ttataacaat
     3541 ggctaagtat ggtgaaaatg gacctggtga tcacgggact gtacaacact caatatttac
     3601 attaaatgga caagtattca tggcgattga tgctaatagt ggcacagaat taccaatgaa
     3661 tcctgcgatt tcattatttg ttacagtaaa agatactatt gaaatggaac gactatttaa
     3721 tggattaaaa gatgaaggtg ccattttaat gccaaaaacg aatatgccac catacagaga
     3781 gtttgctaga tcttcttcaa ctaacggggc aggttagtga cattagaaaa ccgactgtaa
     3841 aaagtacagt cggcattatc tcatattata aaagccagtc attaggccta tctgacaatt
     3901 cctgaataga gttcataaac aatcctgcat gataaccatc acaaacagaa tgatgtacct
     3961 gtaaagatag cggtaaatat attgaattac ctttattaat gaattttcct gctgtaataa
     4021 tgggtagaag gtaattacta ttattattga tatttaagtt aaacccagta aatgaagtcc
     4081 aaggaataat agaaagagaa aaagcatttt caggtatagg tgttttggga aacaatttcc
     4141 ccgaaccatt atatttctct acatcagaaa ggtataaatc ataaaactct ttgaagtcat
     4201 tctttacagg agtccaaata ccagagaatg ttttagatac accatcaaaa attgtataaa
     4261 gtggctctaa cttatcccaa taacctaact ctccgtcgct attgtaacca gttctaaaag
     4321 ctgtatttga gtttatcacc cttgtcacta agaaaataaa tgcagggtaa aatttatatc
     4381 cttcctgttt tatgtttcgg tataaaacac taatatcaat ttctgtggtt atactaaaag
     4441 tcgtttgttg gttcaaataa tgattaaata tctcttttct cttccaattg tctaaatcaa
     4501 ttttattaaa gttcatttga tgtgcctcct aaatttttat ctaaagtgaa tttaggaggc
     4561 ttacttgtct gctttcttca ttagaatcaa tcctttttta aaagtcaata ttactgtaac
     4621 ataaatatat attttaaaaa tatcccactt tatccaattt tcgtttgttg aactaatggg
     4681 tgctttagtt gaagaataaa agaccacatt aaaaaatgtg gtcttttgtg tttttttaaa
     4741 ggatttgagc gtagcgaaaa atccttttct ttcttatctt gagaatacat ggatctgctg
     4801 cattaatgaa tcggccaacg cgcggggaga ggcggtttgc gtattgggcg ctcttccgct
     4861 tcctcgctca ctgactcgct gcgctcggtc gttcggctgc ggcgcgccgt cggcttaaac
     4921 cagttttcgt tggtgcgaaa aaagagtgtc ttgtgacact cttaaattca aaatctatcg
     4981 gtcagattta taccgatttg attttatata ttcttgaata acatacgccg agttatcaca
     5041 taaaagcggg aaccaatcat caaatttaaa cttcattgca taatccatta aactcttaaa
     5101 ttctacgatt ccttgttcat caataaactc aatcatttct ttaattaatt tatatctatc
     5161 tgttgttgtt ttctttaata attcatcaac atctacaccg ccataaacta tcatatcttc
     5221 tttttgatat ttaaatttat taggatcgtc catgtgaagc atatatctca caagaccttt
     5281 cacacttcct gcaatctgcg gaatagtcgc attcaattct tctgtaatta tttttatctg
     5341 ttcataagat ttattaccct catacatcac tagaatatga taatgctctt ttttcatcct
     5401 atcttctgta tcagtatccc tatcatgtaa tggagacact acaaattgaa tgtgtaactc
     5461 ttttaaatac tctaaccact cggcttttgc tgattctgga tataaaacaa atgtccaatt
     5521 acgtcctctt gaatttttct tgttttcagt ttcttttatt acattttcgc tcatgatata
     5581 ataacggtgc taatacattt aacaaaattt agtcatagat aggcagcatg ccagtgctgt
     5641 ctatcttttt ttgtttaaaa tgcaccgtat tcctcctttg catatttttt tattagaata
     5701 ccggttgcat ctgatttgct aatattatat ttttctttga ttctatttaa tatctcattt
     5761 tcttctgttg taagtcttaa agtaacagca acttttttct cttcttttct atctacaacc
     5821 atcactgtac ctcccaacat ctgttttttt cactttaaca taaaaaacaa ccttttaaca
     5881 ttaaaaaccc aatatttatt tatttgtttg gacaatggac aatggacacc taggggggag
     5941 gtcgtagtac ccccctatgt tttctcccct aaataacccc aaaaatctaa gaaaaaaaga
     6001 cctcaaaaag gtctttaatt aacatctcaa atttcgcatt tattccaatt tcctttttgc
     6061 gtgtggcgcg ccggcacgcg tatcgatgct cactcaaagg cggtaatacg gttatccaca
     6121 gaatcagggg ataacgcagg aaagaacatg tgagcaaaag gccagcaaaa ggccaggaac
     6181 cgtaaaaagg ccgcgttgct ggcgtttttc cataggctcc gcccccctga cgagcatcac
     6241 aaaaatcgac gctcaagtca gaggtggcga aacccgacag gactataaag ataccaggcg
     6301 tttccccctg gaagctccct cgtgcgctct cctgttccga ccctgccgct taccggatac
     6361 ctgtccgcct ttctcccttc gggaagcgtg gcgctttctc atagctcacg ctgtaggtat
     6421 ctcagttcgg tgtaggtcgt tcgctccaag ctgggctgtg tgcacgaacc ccccgttcag
     6481 cccgaccgct gcgccttatc cggtaactat cgtcttgagt ccaacccggt aagacacgac
     6541 ttatcgccac tggcagcagc cactggtaac aggattagca gagcgaggta tgtaggcggt
     6601 gctacagagt tcttgaagtg gtggcctaac tacggctaca ctagaaggac agtatttggt
     6661 atctgcgctc tgctgaagcc agttaccttc ggaaaaagag ttggtagctc ttgatccggc
     6721 aaacaaacca ccgctggtag cggtggtttt tttgtttgca agcagcagat tacgcgcaga
     6781 aaaaaaggat ctcaagaaga tcctttgatc ttttctacgg ggtctgacgc tcagtggaac
     6841 gaaaactcac gttaagggat tttggtcatg agattatcaa aaaggatctt cacctagatc
     6901 cttttaaatt aaaaatgaag ttttaaatca atctaaagta tatatgagta aacttggtct
     6961 gacagttacc aatgcttaat cagtgaggca cctatctcag cgatctgtct atttcgttca
     7021 tccatagttg cctgactccc cgtcgtgtag ataactacga tacgggaggg cttaccatct
     7081 ggccccagtg ctgcaatgat accgcgagac ccacgctcac cggctccaga tttatcagca
     7141 ataaaccagc cagccggaag ggccgagcgc agaagtggtc ctgcaacttt atccgcctcc
     7201 atccagtcta ttaattgttg ccgggaagct agagtaagta gttcgccagt taatagtttg
     7261 cgcaacgttg ttgccattgc tacaggcatc gtggtgtcac gctcgtcgtt tggtatggct
     7321 tcattcagct ccggttccca acgatcaagg cgagttacat gatcccccat gttgtgcaaa
     7381 aaagcggtta gctccttcgg tcctccgatc gttgtcagaa gtaagttggc cgcagtgtta
     7441 tcactcatgg ttatggcagc actgcataat tctcttactg tcatgccatc cgtaagatgc
     7501 ttttctgtga ctggtgagta ctcaaccaag tcattctgag aatagtgtat gcggcgaccg
     7561 agttgctctt gcccggcgtc aatacgggat aataccgcgc cacatagcag aactttaaaa
     7621 gtgctcatca ttggaaaacg ttcttcgggg cgaaaactct caaggatctt accgctgttg
     7681 agatccagtt cgatgtaacc cactcgtgca cccaactgat cttcagcatc ttttactttc
     7741 accagcgttt ctgggtgagc aaaaacagga aggcaaaatg ccgcaaaaaa gggaataagg
     7801 gcgacacgga aatgttgaat actcatactc ttcctttttc aatattattg aagcatttat
     7861 cagggttatt gtctcatgag cggatacata tttgaatgta tttagaaaaa taaacaaata
     7921 ggggttccgc gcacatttcc ccgaaaagtg ccacctgacg tctaagaaac cattattatc
     7981 atgacattaa cctataaaaa taggcgtatc acgaggccct ttcgtc
//
