LOCUS       V003697                 7078 bp    DNA     circular SYN 18-DEC-2018
DEFINITION  Exported.
ACCESSION   V003697
VERSION     V003697
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
            .
REFERENCE   1  (bases 1 to 7078)
  AUTHORS   Hsu TM, Welner DH, Russ ZN, Cervantes B, Prathuri RL, Adams PD,
            Dueber JE.
  TITLE     Employing a biochemical protecting group for a sustainable indigo
            dyeing strategy
  JOURNAL   Nat. Chem. Biol. (2018) In press
   PUBMED   29309053
REFERENCE   2  (bases 1 to 7078)
  AUTHORS   Hsu TM, Welner DH, Russ ZN, Cervantes B, Prathuri RL, Adams PD,
            Dueber JE.
  TITLE     Direct Submission
  JOURNAL   Submitted (20-AUG-2017) Bioengineering, UC Berkeley, 2151 Berkeley
            Way Room 512D, Berkeley, CA 94704, USA
REFERENCE   3  (bases 1 to 7078)
  TITLE     Direct Submission
REFERENCE   4  (bases 1 to 7078)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat. Chem.
            Biol. (2018) In press"
            SGRef: number: 2; type: "Journal Article"; journalName: "Submitted
            (20-AUG-2017) Bioengineering, UC Berkeley, 2151 Berkeley Way Room
            512D, Berkeley, CA 94704, USA"
            SGRef: number: 3; type: "Journal Article"
FEATURES             Location/Qualifiers
     source          1..7078
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     misc_feature    complement(168..310)
                     /label="bom"
                     /note="basis of mobility region from pBR322"
     CDS             complement(415..603)
                     /label="rop"
                     /note="Rop protein, which maintains plasmids at low copy
                     number"
     protein_bind    complement(1881..1902)
                     /label="CAP binding site"
                     /note="CAP binding activates transcription in the presence
                     of cAMP."
     CDS             complement(1918..2997)
                     /label="lacI"
                     /note="lac repressor"
     promoter        complement(2998..3075)
                     /label="lacI promoter"
     promoter        3384..3402
                     /label="T7 promoter"
                     /note="promoter for bacteriophage T7 RNA polymerase"
     protein_bind    3403..3427
                     /label="lac operator"
                     /note="The lac repressor binds to the lac operator to
                     inhibit transcription in E. coli. This inhibition can be
                     relieved by adding lactose or
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     RBS             3442..3464
                     /label="RBS"
                     /note="efficient ribosome binding site from bacteriophage
                     T7 gene 10 (Olins and Rangwala, 1989)"
     CDS             3477..3494
                     /label="6xHis"
                     /note="6xHis affinity tag"
     CDS             3516..3536
                     /label="TEV site"
                     /note="tobacco etch virus (TEV) protease recognition and
                     cleavage site"
     CDS             3540..4889
                     /gene="bglA"
                     /label="Beta-glucosidase"
                     /note="Beta-glucosidase from Niallia circulans. Accession#:
                     Q03506"
     terminator      4973..5020
                     /label="T7 terminator"
                     /note="transcription terminator for bacteriophage T7 RNA
                     polymerase"
     promoter        complement(5195..5223)
                     /label="tet promoter"
                     /note="E. coli promoter for tetracycline efflux protein
                     gene"
     promoter        5336..5440
                     /label="AmpR promoter"
     CDS             5441..6298
                     /label="AmpR"
                     /note="beta-lactamase"
     regulatory      6299..6395
                     /regulatory_class="terminator"
     rep_origin      6472..7060
                     /direction=RIGHT
                     /label="ori"
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
                     replication"
ORIGIN
        1 tttttacggt tcctggcctt ttgctggcct tttgctcaca tgttctttcc tgcgttatcc
       61 cctgattctg tggataaccg tattaccgcc tttgagtgag ctgataccgc tcgccgcagc
      121 cgaacgaccg agcgcagcga gtcagtgagc gaggaagcgg aagagcgcct gatgcggtat
      181 tttctcctta cgcatctgtg cggtatttca caccgcatat atggtgcact ctcagtacaa
      241 tctgctctga tgccgcatag ttaagccagt atacactccg ctatcgctac gtgactgggt
      301 catggctgcg ccccgacacc cgccaacacc cgctgacgcg ccctgacggg cttgtctgct
      361 cccggcatcc gcttacagac aagctgtgac cgtctccggg agctgcatgt gtcagaggtt
      421 ttcaccgtca tcaccgaaac gcgcgaggca gctgcggtaa agctcatcag cgtggtcgtg
      481 aagcgattca cagatgtctg cctgttcatc cgcgtccagc tcgttgagtt tctccagaag
      541 cgttaatgtc tggcttctga taaagcgggc catgttaagg gcggtttttt cctgtttggt
      601 cactgatgcc tccgtgtaag ggggatttct gttcatgggg gtaatgatac cgatgaaacg
      661 agagaggatg ctcacgatac gggttactga tgatgaacat gcccggttac tggaacgttg
      721 tgagggtaaa caactggcgg tatggatgcg gcgggaccag agaaaaatca ctcagggtca
      781 atgccagcgc ttcgttaata cagatgtagg tgttccacag ggtagccagc agcatcctgc
      841 gatgcagatc cggaacataa tggtgcaggg cgctgacttc cgcgtttcca gactttacga
      901 aacacggaaa ccgaagacca ttcatgttgt tgctcaggtc gcagacgttt tgcagcagca
      961 gtcgcttcac gttcgctcgc gtatcggtga ttcattctgc taaccagtaa ggcaaccccg
     1021 ccagcctagc cgggtcctca acgacaggag cacgatcatg cgcacccgtg gccaggaccc
     1081 aacgctgccc gagatgcgcc gcgtgcggct gctggagatg gcggacgcga tggatatgtt
     1141 ctgccaaggg ttggtttgcg cattcacagt tctccgcaag aattgattgg ctccaattct
     1201 tggagtggtg aatccgttag cgaggtgccg ccggcttcca ttcaggtcga ggtggcccgg
     1261 ctccatgcac cgcgacgcaa cgcggggagg cagacaaggt atagggcggc gcctacaatc
     1321 catgccaacc cgttccatgt gctcgccgag gcggcataaa tcgccgtgac gatcagcggt
     1381 ccagtgatcg aagttaggct ggtaagagcc gcgagcgatc cttgaagctg tccctgatgg
     1441 tcgtcatcta cctgcctgga cagcatggcc tgcaacgcgg gcatcccgat gccgccggaa
     1501 gcgagaagaa tcataatggg gaaggccatc cagcctcgcg tcgcgaacgc cagcaagacg
     1561 tagcccagcg cgtcggccgc catgccggcg ataatggcct gcttctcgcc gaaacgtttg
     1621 gtggcgggac cagtgacgaa ggcttgagcg agggcgtgca agattccgaa taccgcaagc
     1681 gacaggccga tcatcgtcgc gctccagcga aagcggtcct cgccgaaaat gacccagagc
     1741 gctgccggca cctgtcctac gagttgcatg ataaagaaga cagtcataag tgcggcgacg
     1801 atagtcatgc cccgcgccca ccggaaggag ctgactgggt tgaaggctct caagggcatc
     1861 ggtcgagatc ccggtgccta atgagtgagc taacttacat taattgcgtt gcgctcactg
     1921 cccgctttcc agtcgggaaa cctgtcgtgc cagctgcatt aatgaatcgg ccaacgcgcg
     1981 gggagaggcg gtttgcgtat tgggcgccag ggtggttttt cttttcacca gtgagacggg
     2041 caacagctga ttgcccttca ccgcctggcc ctgagagagt tgcagcaagc ggtccacgct
     2101 ggtttgcccc agcaggcgaa aatcctgttt gatggtggtt aacggcggga tataacatga
     2161 gctgtcttcg gtatcgtcgt atcccactac cgagatatcc gcaccaacgc gcagcccgga
     2221 ctcggtaatg gcgcgcattg cgcccagcgc catctgatcg ttggcaacca gcatcgcagt
     2281 gggaacgatg ccctcattca gcatttgcat ggtttgttga aaaccggaca tggcactcca
     2341 gtcgccttcc cgttccgcta tcggctgaat ttgattgcga gtgagatatt tatgccagcc
     2401 agccagacgc agacgcgccg agacagaact taatgggccc gctaacagcg cgatttgctg
     2461 gtgacccaat gcgaccagat gctccacgcc cagtcgcgta ccgtcttcat gggagaaaat
     2521 aatactgttg atgggtgtct ggtcagagac atcaagaaat aacgccggaa cattagtgca
     2581 ggcagcttcc acagcaatgg catcctggtc atccagcgga tagttaatga tcagcccact
     2641 gacgcgttgc gcgagaagat tgtgcaccgc cgctttacag gcttcgacgc cgcttcgttc
     2701 taccatcgac accaccacgc tggcacccag ttgatcggcg cgagatttaa tcgccgcgac
     2761 aatttgcgac ggcgcgtgca gggccagact ggaggtggca acgccaatca gcaacgactg
     2821 tttgcccgcc agttgttgtg ccacgcggtt gggaatgtaa ttcagctccg ccatcgccgc
     2881 ttccactttt tcccgcgttt tcgcagaaac gtggctggcc tggttcacca cgcgggaaac
     2941 ggtctgataa gagacaccgg catactctgc gacatcgtat aacgttactg gtttcacatt
     3001 caccaccctg aattgactct cttccgggcg ctatcatgcc ataccgcgaa aggttttgcg
     3061 ccattcgatg gtgtccggga tctcgacgct ctcccttatg cgactcctgc attaggaagc
     3121 agcccagtag taggttgagg ccgttgagca ccgccgccgc aaggaatggt gcatgcaagg
     3181 agatggcgcc caacagtccc ccggccacgg ggcctgccac catacccacg ccgaaacaag
     3241 cgctcatgag cccgaagtgg cgagcccgat cttccccatc ggtgatgtcg gcgatatagg
     3301 cgccagcaac cgcacctgtg gcgccggtga tgccggccac gatgcgtccg gcgtagagga
     3361 tcgagatctc gatcccgcga aattaatacg actcactata ggggaattgt gagcggataa
     3421 caattcccct ctagaaataa ttttgtttaa ctttaagaag gagatatacc atgggccatc
     3481 accatcacca tcacgactac gacatcccga ctaccgaaaa cctgtacttc cagggatcta
     3541 tgtctatcca tatgtttcca tctgatttta agtggggtgt tgctacagca gcttatcaaa
     3601 tcgagggtgc ttacaacgaa gacggaaggg gtatgtcaat ttgggacact tttgctcaca
     3661 caccaggtaa agtcaagaat ggtgacaacg gtaacgttgc atgcgattct tatcatagag
     3721 ttgaagaaga tgttcaatta ttaaaagact taggtgttaa agtttacaga ttttctattt
     3781 cttggccaag agtcttgcct cagggaaccg gtgaggttaa tagagcaggt ttggactact
     3841 atcacagatt agtcgatgaa ttgttagcca acggaattga gccattctgc acattgtatc
     3901 attgggattt gccacaagca ttgcaagacc aaggtggttg gggttctagg ataacaatag
     3961 acgcctttgc tgaatatgct gaattaatgt ttaaggagtt aggtggtaag attaaacagt
     4021 ggatcacttt taatgagcca tggtgtatgg cattcttgtc taactatttg ggtgttcacg
     4081 cacctggtaa caaggattta caattagcca ttgatgtttc acatcattta ttagtagccc
     4141 acggaagggc agtcacatta ttcagggaat tgggaatatc tggagaaatc ggtatcgccc
     4201 ctaacacatc ttgggcagtc ccttacagaa gaacaaaaga agacatggag gcctgtttga
     4261 gagtcaacgg atggtctggt gactggtatt tagaccctat ctacttcggt gagtacccaa
     4321 agttcatgtt ggattggtac gaaaatttgg gttataagcc acctattgtt gatggtgata
     4381 tggaattaat tcatcagcct atagacttta ttggtattaa ctattatact tcatcaatga
     4441 atagatacaa tcctggtgaa gcaggtggta tgttgtcttc tgaagcaata tcaatgggag
     4501 cccctaaaac agatataggt tgggaaatat atgctgaagg tttatatgat ttgttgagat
     4561 acacagctga taaatacgga aatccaacct tatacataac agaaaacgga gcatgttata
     4621 acgacggatt atcattagat ggaagaatac atgatcaaag aagaatcgat tatttagcaa
     4681 tgcatttaat ccaagcctca agggcaatcg aagatggaat taatttgaag ggatacatgg
     4741 aatggtcatt aatggacaat tttgaatggg ctgaaggata tggtatgaga tttggtttgg
     4801 ttcatgttga ttatgataca ttagttagaa ctcctaagga ttcattttac tggtataaag
     4861 gtgttatttc aagaggatgg ttggatttag gatcctaact cgagtgatca atccggctgc
     4921 taacaaagcc cgaaaggaag ctgagttggc tgctgccacc gctgagcaat aactagcata
     4981 accccttggg gcctctaaac gggtcttgag gggttttttg ctgaaaggag gaactatatc
     5041 cggatatccc gcaagaggcc cggcagtacc ggcataacca agcctatgcc tacagcatcc
     5101 agggtgacgg tgccgaggat gacgatgagc gcattgttag atttcataca cggtgcctga
     5161 ctgcgttagc aatttaactg tgataaacta ccgcattaaa gcttatcgat gataagctgt
     5221 caaacatgag aattcttgaa gacgaaaggg cctcgtgata cgcctatttt tataggttaa
     5281 tgtcatgata ataatggttt cttagacgtc aggtggcact tttcggggaa atgtgcgcgg
     5341 aacccctatt tgtttatttt tctaaataca ttcaaatatg tatccgctca tgagacaata
     5401 accctgataa atgcttcaat aatattgaaa aaggaagagt atgagtattc aacatttccg
     5461 tgtcgccctt attccctttt ttgcggcatt ttgccttcct gtttttgctc acccagaaac
     5521 gctggtgaaa gtaaaagatg ctgaagatca gttgggtgca cgagtgggtt acatcgaact
     5581 ggatctcaac agcggtaaga tccttgagag ttttcgcccc gaagaacgtt ttccaatgat
     5641 gagcactttt aaagttctgc tatgtggcgc ggtattatcc cgtgttgacg ccgggcaaga
     5701 gcaactcggt cgccgcatac actattctca gaatgacttg gttgagtact caccagtcac
     5761 agaaaagcat cttacggatg gcatgacagt aagagaatta tgcagtgctg ccataaccat
     5821 gagtgataac actgcggcca acttacttct gacaacgatc ggaggaccga aggagctaac
     5881 cgcttttttg cacaacatgg gggatcatgt aactcgcctt gatcgttggg aaccggagct
     5941 gaatgaagcc ataccaaacg acgagcgtga caccacgatg cctgcagcaa tggcaacaac
     6001 gttgcgcaaa ctattaactg gcgaactact tactctagct tcccggcaac aattaataga
     6061 ctggatggag gcggataaag ttgcaggacc acttctgcgc tcggcccttc cggctggctg
     6121 gtttattgct gataaatctg gagccggtga gcgtgggtct cgcggtatca ttgcagcact
     6181 ggggccagat ggtaagccct cccgtatcgt agttatctac acgacgggga gtcaggcaac
     6241 tatggatgaa cgaaatagac agatcgctga gataggtgcc tcactgatta agcattggta
     6301 actgtcagac caagtttact catatatact ttagattgat ttaaaacttc atttttaatt
     6361 taaaaggatc taggtgaaga tcctttttga taatctcatg accaaaatcc cttaacgtga
     6421 gttttcgttc cactgagcgt cagaccccgt agaaaagatc aaaggatctt cttgagatcc
     6481 tttttttctg cgcgtaatct gctgcttgca aacaaaaaaa ccaccgctac cagcggtggt
     6541 ttgtttgccg gatcaagagc taccaactct ttttccgaag gtaactggct tcagcagagc
     6601 gcagatacca aatactgtcc ttctagtgta gccgtagtta ggccaccact tcaagaactc
     6661 tgtagcaccg cctacatacc tcgctctgct aatcctgtta ccagtggctg ctgccagtgg
     6721 cgataagtcg tgtcttaccg ggttggactc aagacgatag ttaccggata aggcgcagcg
     6781 gtcgggctga acggggggtt cgtgcacaca gcccagcttg gagcgaacga cctacaccga
     6841 actgagatac ctacagcgtg agctatgaga aagcgccacg cttcccgaag ggagaaaggc
     6901 ggacaggtat ccggtaagcg gcagggtcgg aacaggagag cgcacgaggg agcttccagg
     6961 gggaaacgcc tggtatcttt atagtcctgt cgggtttcgc cacctctgac ttgagcgtcg
     7021 atttttgtga tgctcgtcag gggggcggag cctatggaaa aacgccagca acgcggcc
//
