LOCUS       V003699                 7090 bp    DNA     circular SYN 18-DEC-2018
DEFINITION  Exported.
ACCESSION   V003699
VERSION     V003699
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
            .
REFERENCE   1  (bases 1 to 7090)
  AUTHORS   Redder P, Linder P.
  TITLE     New Range of Vectors with a Stringent 5-Fluoroorotic Acid-Based
            Counterselection System for Generating Mutants by Allelic
            Replacement in Staphylococcus aureus
  JOURNAL   Appl. Environ. Microbiol. 78 (11), 3846-3854 (2012)
   PUBMED   22447609
REFERENCE   2  (bases 1 to 7090)
  AUTHORS   Redder P, Linder P.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2012) Department of Microbiology and Molecular
            Medicine, University of Geneva, Faculty of Medicine, Rue
            Michel-Servet 1, Geneva 1203, Switzerland
REFERENCE   3  (bases 1 to 7090)
  TITLE     Direct Submission
REFERENCE   4  (bases 1 to 7090)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     ##Assembly-Data-START##
            Assembly Method       :: BioEdit, CAP v. 7.0.9.0
            Sequencing Technology :: Sanger dideoxy sequencing
            ##Assembly-Data-END##
            SGRef: number: 1; type: "Journal Article"; journalName: "Appl.
            Environ. Microbiol."; date: "2012"; volume: "78"; issue: "11";
            pages: "3846-3854"
            SGRef: number: 2; type: "Journal Article"; journalName: "Submitted
            (12-MAR-2012) Department of Microbiology and Molecular Medicine,
            University of Geneva, Faculty of Medicine, Rue Michel-Servet 1,
            Geneva 1203, Switzerland"
            SGRef: number: 3; type: "Journal Article"
FEATURES             Location/Qualifiers
     source          1..7090
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     rep_origin      complement(50..1209)
                     /direction=LEFT
                     /note="thermosensitive origin of replication; for
                     replication in Staphylococcus aureus; pE194ts"
     misc_feature    1210..1865
                     /note="fragment of the pyrFE genes from Staphylococcus
                     aureus; replacing the pC194 origin of replication"
     CDS             2156..2887
                     /gene="ermC"
                     /label="rRNA adenine N-6-methyltransferase"
                     /note="rRNA adenine N-6-methyltransferase from
                     Staphylococcus aureus. Accession#: P02979"
     misc_feature    complement(3021..3128)
                     /label="MCS"
                     /note="pBluescript multiple cloning site"
     promoter        complement(3137..3155)
                     /label="T7 promoter"
                     /note="promoter for bacteriophage T7 RNA polymerase"
     rep_origin      3604..3826
                     /label="pSC101 ori"
                     /note="low-copy replication origin that requires the Rep101
                     protein"
     CDS             3874..4821
                     /label="Rep101"
                     /note="RepA protein needed for replication with the pSC101
                     origin"
     CDS             6076..6864
                     /label="SmR"
                     /note="aminoglycoside adenylyltransferase (Murphy, 1985)"
ORIGIN
        1 ggcgcgccag atcgcgtgca taattcaaca gctaacctcc caataactac gcgtcggctt
       61 aaaccagttt tcgttggtgc gaaaaaagag tgtcttgtga cactcttaaa ttcaaaatct
      121 atcggtcaga tttataccga tttgatttta tatattcttg aataacatac gccgagttat
      181 cacataaaag cgggaaccaa tcatcaaatt taaacttcat tgcataatcc attaaactct
      241 taaattctac gattccttgt tcatcaataa actcaatcat ttctttaatt aatttatatc
      301 tatctgttgt tgttttcttt aataattcat caacatctac accgccataa actatcatat
      361 cttctttttg atatttaaat ttattaggat cgtccatgtg aagcatatat ctcacaagac
      421 ctttcacact tcctgcaatc tgcggaatag tcgcattcaa ttcttctgta attattttta
      481 tctgttcata agatttatta ccctcataca tcactagaat atgataatgc tcttttttca
      541 tcctatcttc tgtatcagta tccctatcat gtaatggaga cactacaaat tgaatgtgta
      601 actcttttaa atactctaac cactcggctt ttgctgattc tggatataaa acaaatgtcc
      661 aattacgtcc tcttgaattt ttcttgtttt cagtttcttt tattacattt tcgctcatga
      721 tataataacg gtgctaatac atttaacaaa atttagtcat agataggcag catgccagtg
      781 ctgtctatct ttttttgttt aaaatgcacc gtattcctcc tttgcatatt tttttattag
      841 aataccggtt gcatctgatt tgctaatatt atatttttct ttgattctat ttaatatctc
      901 attttcttct gttgtaagtc ttaaagtaac agcaactttt ttctcttctt ttctatctac
      961 aaccatcact gtacctccca acatctgttt ttttcacttt aacataaaaa acaacctttt
     1021 aacattaaaa acccaatatt tatttatttg tttggacaat ggacaatgga cacctagggg
     1081 ggaggtcgta gtacccccct atgttttctc ccctaaataa ccccaaaaat ctaagaaaaa
     1141 aagacctcaa aaaggtcttt aattaacatc tcaaatttcg catttattcc aatttccttt
     1201 ttgcgtgtcc atggtacagt tggtaaggcg atggaaggac tagctaaatt gaatgttgat
     1261 ctggtaaatg ttcatgctgc tggtggcgta aaaatgatgt ctgaggccat taaaggatta
     1321 agaaaacata atcaacatac aaaaattatt gcagtaacac agcttacgtc aacaacagaa
     1381 gacatgttac gacacgaaca aaatatacaa acatcgattg aagaggccgt tttaaattat
     1441 gccaagttag caaatgcagc tggtttagat ggcgttgttt gttcacctct tgaaagtcgt
     1501 atgttgactg aaaagttagg tacatcattt ttaaaagtaa caccaggtat tagacctaaa
     1561 ggtgcatctc aagatgacca acaccgtatt acgacaccgg aagaagcaag acagcttggt
     1621 tcgacgcata ttgtagtcgg tagaccgatt acacaaagtg acaatccagt cgaaagttat
     1681 cataaaatta aagaaagttg gttagtataa tggctaaaga aattgcaaaa tcattattag
     1741 atattgaagc tgtaacatta tcaccaaatg atttatatac atggagttca ggtattaaat
     1801 caccgattta ctgtgataac cgtgttacgt taggttatcc tttagttcga ggcgcaatcc
     1861 gcgatgtaca tttaagaact ttcttttttt acaagaaaaa agaaattaga taaatctctc
     1921 atatctttta ttcaataatc gcatccgatt gcagtataaa tttaacgatc actcatcatg
     1981 ttcattttat cagagcttgt gctataatta tactaatttt ataaggagga aaaaatatgg
     2041 gcatttttag tatttttgta atcagcacag ttcattatca accaaacaaa aaataagtgg
     2101 ttataatgaa tcgttaataa gcaaaattca tataaccaaa ttaaagaggg ttataatgaa
     2161 cgagaaaaat ataaaacaca gtcaaaactt tattacttca aaacataata tagataaaat
     2221 aatgacaaat ataagattaa atgaacatga taatatcttt gaaatcggct caggaaaagg
     2281 ccattttacc cttgaattag taaagaggtg taatttcgta actgccattg aaatagacca
     2341 taaattatgc aaaactacag aaaataaact tgttgatcac gataatttcc aagttttaaa
     2401 caaggatata ttgcagttta aatttcctaa aaaccaatcc tataaaatat atggtaatat
     2461 accttataac ataagtacgg atataatacg caaaattgtt tttgatagta tagctaatga
     2521 gatttattta atcgtggaat acgggtttgc taaaagatta ttaaatacaa aacgctcatt
     2581 ggcattactt ttaatggcag aagttgatat ttctatatta agtatggttc caagagaata
     2641 ttttcatcct aaacctaaag tgaatagctc acttatcaga ttaagtagga aaaaatcaag
     2701 aatatcacac aaagataaac aaaagtataa ttatttcgtt atgaaatggg ttaacaaaga
     2761 atacaagaaa atatttacaa aaaatcaatt taacaattcc ttaaaacatg caggaattga
     2821 cgatttaaac aatattagct ttgaacaatt cttatctctt ttcaatagct ataaattatt
     2881 taataagtaa gttaagggat gcataaactg catcccttaa cttgtttttc gtgtgcctat
     2941 tttttgtgtt cgaatgcccc gttagttgaa gaaggttttt atattacagc tccagatctc
     3001 actaaaggga acaaaagctg ggtaccgggc cccccctcga ggtcgacggt atcgataagc
     3061 ttgatatcga attcctgcag cccgggggat ccactagttc tagagcggcc gccaccgcgg
     3121 tggagctcca attcgcccta tagtgagtcg tattaggcgc gccttactgg gtgcattagc
     3181 cagtctgaat gacctgtcac gggataatcc gaagtggtca gactggaaaa tcagagggca
     3241 ggaactgctg aacagcaaaa agtcagatag caccacatag cagacccgcc ataaaacgcc
     3301 ctgagaagcc cgtgacgggc ttttcttgta ttatgggtag tttccttgca tgaatccata
     3361 aaaggcgcct gtagtgccat ttacccccat tcactgccag agccgtgagc gcagcgaact
     3421 gaatgtcacg aaaaagacag cgactcaggt gcctgatggt cggagacaaa aggaatattc
     3481 agcgatttgc ccgagcttgc gagggtgcta cttaagcctt tagggtttta aggtctgttt
     3541 tgtagaggag caaacagcgt ttgcgacatc cttttgtaat actgcggaac tgactaaagt
     3601 agtgagttat acacagggct gggatctatt ctttttatct ttttttattc tttctttatt
     3661 ctataaatta taaccacttg aatataaaca aaaaaaacac acaaaggtct agcggaattt
     3721 acagagggtc tagcagaatt tacaagtttt ccagcaaagg tctagcagaa tttacagata
     3781 cccacaactc aaaggaaaag gactagtaat tatcattgac tagcccatct caattggtat
     3841 agtgattaaa atcacctaga ccaattgaga tgtatgtctg aattagttgt tttcaaagca
     3901 aatgaactag cgattagtcg ctatgactta acggagcatg aaaccaagct aattttatgc
     3961 tgtgtggcac tactcaaccc cacgattgaa aaccctacaa ggaaagaacg gacggtatcg
     4021 ttcacttata accaatacgc tcagatgatg aacatcagta gggaaaatgc ttatggtgta
     4081 ttagctaaag caaccagaga gctgatgacg agaactgtgg aaatcaggaa tcctttggtt
     4141 aaaggcttta agattttcca gtggacaaac tatgccaagt tctcaagcga aaaattagaa
     4201 ttagttttta gtgaagagat attgccttat cttttccagt taaaaaaatt cataaaatat
     4261 aatctggaac atgttaagtc ttttgaaaac aaatactcta tgaggattta tgagtggtta
     4321 ttaaaagaac taacacaaaa gaaaactcac aaggcaaata tagagattag ccttgatgaa
     4381 tttaagttca tgttaatgct tgaaaataac taccatgagt ttaaaaggct taaccaatgg
     4441 gttttgaaac caataagtaa agatttaaac acttacagca atatgaaatt ggtggttgat
     4501 aagcgaggcc gcccgactga tacgttgatt ttccaagttg aactagatag acaaatggat
     4561 ctcgtaaccg aacttgagaa caaccagata aaaatgaatg gtgacaaaat accaacaacc
     4621 attacatcag attcctacct acgtaacgga ctaagaaaaa cactacacga tgctttaact
     4681 gcaaaaattc agctcaccag ttttgaggca aaatttttga gtgacatgca aagtaagtat
     4741 gatctcaatg gttcgttctc atggctcacg caaaaacaac gaaccacact agagaacata
     4801 ctggctaaat acggaaggat ctgaggttct tatggctctt gtatctatca gtgaagcatc
     4861 aagactaaca aacaaaagta gaacaactgt tcaccgttac atatcaaagg gaaaactgtc
     4921 catatgcaca gatgaaaacg gtgtaaaaaa gatagataca tcagagcttt tacgagtttt
     4981 tggtgcattc aaagctgttc accatgaaca gatcgacaat gtaacagatg aacagcatgt
     5041 aacacctaat agaacaggtg aaaccagtaa aacaaagcaa ctagaacatg aaattgaaca
     5101 cctgagacaa cttgttacag ctcaacagtc acacatagac agcctgaaac aggcgatgct
     5161 gcttatcgaa tcaaagctgc cgacaacacg ggagccagtg acgcctcccg tggggaaaaa
     5221 atcatggcaa ttctggaaga aatagcgctt tcagccggca aaccggctga agccggatct
     5281 gcgattctga taacaaacta gcaacaccag aacagcccgt ttgcgggcag caaaacccgt
     5341 gggaattaat tcccctgctc gcgcaggctg ggtgccaagc tctcgggtaa catcaaggcc
     5401 cgatccttgg agcccttgcc ctcccgcacg atgatcgtgc cgtgatcgaa atccagatcc
     5461 ttgacccgca gttgcaaacc ctcactgatc cgcatgcccg ttccatacag aagctgggcg
     5521 aacaaacgat gctcgccttc cagaaaaccg aggatgcgaa ccacttcatc cggggtcagc
     5581 accaccggca agcgccgcga cggccgaggt cttccgatct cctgaagcca gggcagatcc
     5641 gtgcacagca ccttgccgta gaagaacagc aaggccgcca atgcctgacg atgcgtggag
     5701 accgaaacct tgcgctcgtt cgccagccag gacagaaatg cctcgacttc gctgctgccc
     5761 aaggttgccg ggtgacgcac accgtggaaa cggatgaagg cacgaaccca gtggacataa
     5821 gcctgttcgg ttcgtaagct gtaatgcaag tagcgtatgc gctcacgcaa ctggtccaga
     5881 accttgaccg aacgcagcgg tggtaacggc gcagtggcgg ttttcatggc ttgttatgac
     5941 tgtttttttg gggtacagtc tatgcctcgg gcatccaagc agcaagcgcg ttacgccgtg
     6001 ggtcgatgtt tgatgttatg gagcagcaac gatgttacgc agcagggcag tcgccctaaa
     6061 acaaagttaa acatcatgag ggaagcggtg atcgccgaag tatcgactca actatcagag
     6121 gtagttggcg tcatcgagcg ccatctcgaa ccgacgttgc tggccgtaca tttgtacggc
     6181 tccgcagtgg atggcggcct gaagccacac agtgatattg atttgctggt tacggtgacc
     6241 gtaaggcttg atgaaacaac gcggcgagct ttgatcaacg accttttgga aacttcggct
     6301 tcccctggag agagcgagat tctccgcgct gtagaagtca ccattgttgt gcacgacgac
     6361 atcattccgt ggcgttatcc agctaagcgc gaactgcaat ttggagaatg gcagcgcaat
     6421 gacattcttg caggtatctt cgagccagcc acgatcgaca ttgatctggc tatcttgctg
     6481 acaaaagcaa gagaacatag cgttgccttg gtaggtccag cggcggagga actctttgat
     6541 ccggttcctg aacaggatct atttgaggcg ctaaatgaaa ccttaacgct atggaactcg
     6601 ccgcccgact gggctggcga tgagcgaaat gtagtgctta cgttgtcccg catttggtac
     6661 agcgcagtaa ccggcaaaat cgcgccgaag gatgtcgctg ccgactgggc aatggagcgc
     6721 ctgccggccc agtatcagcc cgtcatactt gaagctagac aggcttatct tggacaagaa
     6781 gaagatcgct tggcctcgcg cgcagatcag ttggaagaat ttgtccacta cgtgaaaggc
     6841 gagatcacca aggtagtcgg caaataatgt ctaacaattc gttcaagccg acgccgcttc
     6901 gcggcgcggc ttaactcaag cgttagatgc actaagcaca taattgctca cagccaaact
     6961 atcaggtcaa gtctgctttt attattttta agcgtgcata ataagcccta cacaaattgg
     7021 gagatatatc atgaaaggct ggctttttct tgttatcgca atagttggcg aagtaatcgc
     7081 aacatccgca
//
