LOCUS       V003700                 7550 bp    DNA     circular SYN 18-DEC-2018
DEFINITION  Exported.
ACCESSION   V003700
VERSION     V003700
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
            .
REFERENCE   1  (bases 1 to 7550)
  AUTHORS   Redder P, Linder P.
  TITLE     New Range of Vectors with a Stringent 5-Fluoroorotic Acid-Based
            Counterselection System for Generating Mutants by Allelic
            Replacement in Staphylococcus aureus
  JOURNAL   Appl. Environ. Microbiol. 78 (11), 3846-3854 (2012)
   PUBMED   22447609
REFERENCE   2  (bases 1 to 7550)
  AUTHORS   Redder P, Linder P.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2012) Department of Microbiology and Molecular
            Medicine, University of Geneva, Faculty of Medicine, Rue
            Michel-Servet 1, Geneva 1203, Switzerland
REFERENCE   3  (bases 1 to 7550)
  TITLE     Direct Submission
REFERENCE   4  (bases 1 to 7550)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     ##Assembly-Data-START##
            Assembly Method       :: BioEdit, CAP v. 7.0.9.0
            Sequencing Technology :: Sanger dideoxy sequencing
            ##Assembly-Data-END##
            SGRef: number: 1; type: "Journal Article"; journalName: "Appl.
            Environ. Microbiol."; date: "2012"; volume: "78"; issue: "11";
            pages: "3846-3854"
            SGRef: number: 2; type: "Journal Article"; journalName: "Submitted
            (12-MAR-2012) Department of Microbiology and Molecular Medicine,
            University of Geneva, Faculty of Medicine, Rue Michel-Servet 1,
            Geneva 1203, Switzerland"
            SGRef: number: 3; type: "Journal Article"
FEATURES             Location/Qualifiers
     source          1..7550
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     rep_origin      complement(50..1669)
                     /direction=LEFT
                     /note="thermosensitive origin of replication; for
                     replication in Staphylococcus aureus.; pT181ts"
     misc_feature    1670..2325
                     /note="fragment of the pyrFE genes from Staphylococcus
                     aureus; replacing the pC194 origin of replication"
     CDS             2616..3347
                     /gene="ermC"
                     /label="rRNA adenine N-6-methyltransferase"
                     /note="rRNA adenine N-6-methyltransferase from
                     Staphylococcus aureus. Accession#: P02979"
     misc_feature    complement(3481..3588)
                     /label="MCS"
                     /note="pBluescript multiple cloning site"
     promoter        complement(3597..3615)
                     /label="T7 promoter"
                     /note="promoter for bacteriophage T7 RNA polymerase"
     rep_origin      4064..4286
                     /label="pSC101 ori"
                     /note="low-copy replication origin that requires the Rep101
                     protein"
     CDS             4334..5281
                     /label="Rep101"
                     /note="RepA protein needed for replication with the pSC101
                     origin"
     CDS             6536..7324
                     /label="SmR"
                     /note="aminoglycoside adenylyltransferase (Murphy, 1985)"
ORIGIN
        1 ggcgcgccag atcgcgtgca taattcaaca gctaacctcc caataactac gcgtctcttt
       61 acttgaggtg actaaagttt ataggggtaa attattcttt cacctaatat aaattcccca
      121 aatttttaaa tagacacttc ataaaaaaat cctcctaaat tagaatagtt taaatatatc
      181 atttttgttc agtaatatta atatgtacgt acactatttc caaaatttaa attcatgttg
      241 ccaaaaatcg atttgttttt gcaattgttt ttcgttcgct tttaaagtcg atttcattaa
      301 gtccgttaaa tcgactggcg aaatttcttt tatcaaattc ttatattttg ttctggaatt
      361 tctgtgtagc tttccccatt cttcttcatc actcaataac ataaaaacta ttgctctatc
      421 cgcagtgcgt tggatagttt tccaatctgg ttgcaagata tgtaaatcac taaagcaatc
      481 attccagtaa tccaccatat ctcttttaaa ttcgatttct acacgccata aatgttcaga
      541 cataacttca gcatctgcat tatctttacg ttcttgcttt ttattataaa ttctaataaa
      601 tctattacta tctctcacgc caaaatattt tgtttctggc ttaccattac gaccataaaa
      661 aatagttttc ttaactgctt tatcagacat tgcatagtag tcactcaaat catcttcaaa
      721 atcaaaggct aaatctaatc ttgtaaaacc gtcatcttcc atgtagctta ttatattttg
      781 ttttaaccaa atcatttcat ctcgtgtaag tttgtttgga ttaaattcaa tacgcatatt
      841 acgtctatcc caactatctg ctttcacttt gtcatattca atataaactt tttcttgaag
      901 tgctttagct ttaaactttg tttgaagaat atcccaaagt cttatttggg gctctacact
      961 cataaattta gaaagggctt gagcgttgtc tcggttgaga tttccaacga tcgtcattgc
     1021 atcaaaactt aattttggat cagatatgca cacagtatgt gcgtccaacc ggctattaga
     1081 gtagccggtt ttagaaaaat tgtctaaatc gtgattttcc aaatgatttg aatgatttgc
     1141 atgattgttt ttatacataa aaaatcgact ccttaatctc aatttcgttt aaggaatcgc
     1201 tcacccaaat atatatcttg atgtatattt aaatatcgtt taatatctaa atatacaaga
     1261 ttataaaaac aactcagtgt ttttttcttt gaatgatgtc gttcacaaac tttggtcagg
     1321 tcgtgagcga ctccttttta ttttgttatt aatataacac tatcaaaaga tttggtctaa
     1381 tcagatcaag tcttttttta tttaagcatt tgtattatct ggtaaacagt taaaactact
     1441 aaaacaccaa gtacatactt acttgttata aaattctcaa aggcttctct gaatttcatt
     1501 tcttcaactc cttttattgc tttattccaa tttccttatt ggtcggaacc tacagggctt
     1561 tatgtgttgt tgagttggta ctttcttggg attaatccca attcaagtcc aaccaactcg
     1621 ctaacaagtt agctaacaca tagcccattc caaccaataa gttttctccc atggtacagt
     1681 tggtaaggcg atggaaggac tagctaaatt gaatgttgat ctggtaaatg ttcatgctgc
     1741 tggtggcgta aaaatgatgt ctgaggccat taaaggatta agaaaacata atcaacatac
     1801 aaaaattatt gcagtaacac agcttacgtc aacaacagaa gacatgttac gacacgaaca
     1861 aaatatacaa acatcgattg aagaggccgt tttaaattat gccaagttag caaatgcagc
     1921 tggtttagat ggcgttgttt gttcacctct tgaaagtcgt atgttgactg aaaagttagg
     1981 tacatcattt ttaaaagtaa caccaggtat tagacctaaa ggtgcatctc aagatgacca
     2041 acaccgtatt acgacaccgg aagaagcaag acagcttggt tcgacgcata ttgtagtcgg
     2101 tagaccgatt acacaaagtg acaatccagt cgaaagttat cataaaatta aagaaagttg
     2161 gttagtataa tggctaaaga aattgcaaaa tcattattag atattgaagc tgtaacatta
     2221 tcaccaaatg atttatatac atggagttca ggtattaaat caccgattta ctgtgataac
     2281 cgtgttacgt taggttatcc tttagttcga ggcgcaatcc gcgatgtaca tttaagaact
     2341 ttcttttttt acaagaaaaa agaaattaga taaatctctc atatctttta ttcaataatc
     2401 gcatccgatt gcagtataaa tttaacgatc actcatcatg ttcattttat cagagcttgt
     2461 gctataatta tactaatttt ataaggagga aaaaatatgg gcatttttag tatttttgta
     2521 atcagcacag ttcattatca accaaacaaa aaataagtgg ttataatgaa tcgttaataa
     2581 gcaaaattca tataaccaaa ttaaagaggg ttataatgaa cgagaaaaat ataaaacaca
     2641 gtcaaaactt tattacttca aaacataata tagataaaat aatgacaaat ataagattaa
     2701 atgaacatga taatatcttt gaaatcggct caggaaaagg ccattttacc cttgaattag
     2761 taaagaggtg taatttcgta actgccattg aaatagacca taaattatgc aaaactacag
     2821 aaaataaact tgttgatcac gataatttcc aagttttaaa caaggatata ttgcagttta
     2881 aatttcctaa aaaccaatcc tataaaatat atggtaatat accttataac ataagtacgg
     2941 atataatacg caaaattgtt tttgatagta tagctaatga gatttattta atcgtggaat
     3001 acgggtttgc taaaagatta ttaaatacaa aacgctcatt ggcattactt ttaatggcag
     3061 aagttgatat ttctatatta agtatggttc caagagaata ttttcatcct aaacctaaag
     3121 tgaatagctc acttatcaga ttaagtagga aaaaatcaag aatatcacac aaagataaac
     3181 aaaagtataa ttatttcgtt atgaaatggg ttaacaaaga atacaagaaa atatttacaa
     3241 aaaatcaatt taacaattcc ttaaaacatg caggaattga cgatttaaac aatattagct
     3301 ttgaacaatt cttatctctt ttcaatagct ataaattatt taataagtaa gttaagggat
     3361 gcataaactg catcccttaa cttgtttttc gtgtgcctat tttttgtgtt cgaatgcccc
     3421 gttagttgaa gaaggttttt atattacagc tccagatctc actaaaggga acaaaagctg
     3481 ggtaccgggc cccccctcga ggtcgacggt atcgataagc ttgatatcga attcctgcag
     3541 cccgggggat ccactagttc tagagcggcc gccaccgcgg tggagctcca attcgcccta
     3601 tagtgagtcg tattaggcgc gccttactgg gtgcattagc cagtctgaat gacctgtcac
     3661 gggataatcc gaagtggtca gactggaaaa tcagagggca ggaactgctg aacagcaaaa
     3721 agtcagatag caccacatag cagacccgcc ataaaacgcc ctgagaagcc cgtgacgggc
     3781 ttttcttgta ttatgggtag tttccttgca tgaatccata aaaggcgcct gtagtgccat
     3841 ttacccccat tcactgccag agccgtgagc gcagcgaact gaatgtcacg aaaaagacag
     3901 cgactcaggt gcctgatggt cggagacaaa aggaatattc agcgatttgc ccgagcttgc
     3961 gagggtgcta cttaagcctt tagggtttta aggtctgttt tgtagaggag caaacagcgt
     4021 ttgcgacatc cttttgtaat actgcggaac tgactaaagt agtgagttat acacagggct
     4081 gggatctatt ctttttatct ttttttattc tttctttatt ctataaatta taaccacttg
     4141 aatataaaca aaaaaaacac acaaaggtct agcggaattt acagagggtc tagcagaatt
     4201 tacaagtttt ccagcaaagg tctagcagaa tttacagata cccacaactc aaaggaaaag
     4261 gactagtaat tatcattgac tagcccatct caattggtat agtgattaaa atcacctaga
     4321 ccaattgaga tgtatgtctg aattagttgt tttcaaagca aatgaactag cgattagtcg
     4381 ctatgactta acggagcatg aaaccaagct aattttatgc tgtgtggcac tactcaaccc
     4441 cacgattgaa aaccctacaa ggaaagaacg gacggtatcg ttcacttata accaatacgc
     4501 tcagatgatg aacatcagta gggaaaatgc ttatggtgta ttagctaaag caaccagaga
     4561 gctgatgacg agaactgtgg aaatcaggaa tcctttggtt aaaggcttta agattttcca
     4621 gtggacaaac tatgccaagt tctcaagcga aaaattagaa ttagttttta gtgaagagat
     4681 attgccttat cttttccagt taaaaaaatt cataaaatat aatctggaac atgttaagtc
     4741 ttttgaaaac aaatactcta tgaggattta tgagtggtta ttaaaagaac taacacaaaa
     4801 gaaaactcac aaggcaaata tagagattag ccttgatgaa tttaagttca tgttaatgct
     4861 tgaaaataac taccatgagt ttaaaaggct taaccaatgg gttttgaaac caataagtaa
     4921 agatttaaac acttacagca atatgaaatt ggtggttgat aagcgaggcc gcccgactga
     4981 tacgttgatt ttccaagttg aactagatag acaaatggat ctcgtaaccg aacttgagaa
     5041 caaccagata aaaatgaatg gtgacaaaat accaacaacc attacatcag attcctacct
     5101 acgtaacgga ctaagaaaaa cactacacga tgctttaact gcaaaaattc agctcaccag
     5161 ttttgaggca aaatttttga gtgacatgca aagtaagtat gatctcaatg gttcgttctc
     5221 atggctcacg caaaaacaac gaaccacact agagaacata ctggctaaat acggaaggat
     5281 ctgaggttct tatggctctt gtatctatca gtgaagcatc aagactaaca aacaaaagta
     5341 gaacaactgt tcaccgttac atatcaaagg gaaaactgtc catatgcaca gatgaaaacg
     5401 gtgtaaaaaa gatagataca tcagagcttt tacgagtttt tggtgcattc aaagctgttc
     5461 accatgaaca gatcgacaat gtaacagatg aacagcatgt aacacctaat agaacaggtg
     5521 aaaccagtaa aacaaagcaa ctagaacatg aaattgaaca cctgagacaa cttgttacag
     5581 ctcaacagtc acacatagac agcctgaaac aggcgatgct gcttatcgaa tcaaagctgc
     5641 cgacaacacg ggagccagtg acgcctcccg tggggaaaaa atcatggcaa ttctggaaga
     5701 aatagcgctt tcagccggca aaccggctga agccggatct gcgattctga taacaaacta
     5761 gcaacaccag aacagcccgt ttgcgggcag caaaacccgt gggaattaat tcccctgctc
     5821 gcgcaggctg ggtgccaagc tctcgggtaa catcaaggcc cgatccttgg agcccttgcc
     5881 ctcccgcacg atgatcgtgc cgtgatcgaa atccagatcc ttgacccgca gttgcaaacc
     5941 ctcactgatc cgcatgcccg ttccatacag aagctgggcg aacaaacgat gctcgccttc
     6001 cagaaaaccg aggatgcgaa ccacttcatc cggggtcagc accaccggca agcgccgcga
     6061 cggccgaggt cttccgatct cctgaagcca gggcagatcc gtgcacagca ccttgccgta
     6121 gaagaacagc aaggccgcca atgcctgacg atgcgtggag accgaaacct tgcgctcgtt
     6181 cgccagccag gacagaaatg cctcgacttc gctgctgccc aaggttgccg ggtgacgcac
     6241 accgtggaaa cggatgaagg cacgaaccca gtggacataa gcctgttcgg ttcgtaagct
     6301 gtaatgcaag tagcgtatgc gctcacgcaa ctggtccaga accttgaccg aacgcagcgg
     6361 tggtaacggc gcagtggcgg ttttcatggc ttgttatgac tgtttttttg gggtacagtc
     6421 tatgcctcgg gcatccaagc agcaagcgcg ttacgccgtg ggtcgatgtt tgatgttatg
     6481 gagcagcaac gatgttacgc agcagggcag tcgccctaaa acaaagttaa acatcatgag
     6541 ggaagcggtg atcgccgaag tatcgactca actatcagag gtagttggcg tcatcgagcg
     6601 ccatctcgaa ccgacgttgc tggccgtaca tttgtacggc tccgcagtgg atggcggcct
     6661 gaagccacac agtgatattg atttgctggt tacggtgacc gtaaggcttg atgaaacaac
     6721 gcggcgagct ttgatcaacg accttttgga aacttcggct tcccctggag agagcgagat
     6781 tctccgcgct gtagaagtca ccattgttgt gcacgacgac atcattccgt ggcgttatcc
     6841 agctaagcgc gaactgcaat ttggagaatg gcagcgcaat gacattcttg caggtatctt
     6901 cgagccagcc acgatcgaca ttgatctggc tatcttgctg acaaaagcaa gagaacatag
     6961 cgttgccttg gtaggtccag cggcggagga actctttgat ccggttcctg aacaggatct
     7021 atttgaggcg ctaaatgaaa ccttaacgct atggaactcg ccgcccgact gggctggcga
     7081 tgagcgaaat gtagtgctta cgttgtcccg catttggtac agcgcagtaa ccggcaaaat
     7141 cgcgccgaag gatgtcgctg ccgactgggc aatggagcgc ctgccggccc agtatcagcc
     7201 cgtcatactt gaagctagac aggcttatct tggacaagaa gaagatcgct tggcctcgcg
     7261 cgcagatcag ttggaagaat ttgtccacta cgtgaaaggc gagatcacca aggtagtcgg
     7321 caaataatgt ctaacaattc gttcaagccg acgccgcttc gcggcgcggc ttaactcaag
     7381 cgttagatgc actaagcaca taattgctca cagccaaact atcaggtcaa gtctgctttt
     7441 attattttta agcgtgcata ataagcccta cacaaattgg gagatatatc atgaaaggct
     7501 ggctttttct tgttatcgca atagttggcg aagtaatcgc aacatccgca
//
