LOCUS       V003701                 5936 bp    DNA     circular SYN 18-DEC-2018
DEFINITION  Exported.
ACCESSION   V003701
VERSION     V003701
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
            .
REFERENCE   1  (bases 1 to 5936)
  AUTHORS   Redder P, Linder P.
  TITLE     New Range of Vectors with a Stringent 5-Fluoroorotic Acid-Based
            Counterselection System for Generating Mutants by Allelic
            Replacement in Staphylococcus aureus
  JOURNAL   Appl. Environ. Microbiol. 78 (11), 3846-3854 (2012)
   PUBMED   22447609
REFERENCE   2  (bases 1 to 5936)
  AUTHORS   Redder P, Linder P.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2012) Department of Microbiology and Molecular
            Medicine, University of Geneva, Faculty of Medicine, Rue
            Michel-Servet 1, Geneva 1203, Switzerland
REFERENCE   3  (bases 1 to 5936)
  TITLE     Direct Submission
REFERENCE   4  (bases 1 to 5936)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     ##Assembly-Data-START##
            Assembly Method       :: BioEdit, CAP v. 7.0.9.0
            Sequencing Technology :: Sanger dideoxy sequencing
            ##Assembly-Data-END##
            SGRef: number: 1; type: "Journal Article"; journalName: "Appl.
            Environ. Microbiol."; date: "2012"; volume: "78"; issue: "11";
            pages: "3846-3854"
            SGRef: number: 2; type: "Journal Article"; journalName: "Submitted
            (12-MAR-2012) Department of Microbiology and Molecular Medicine,
            University of Geneva, Faculty of Medicine, Rue Michel-Servet 1,
            Geneva 1203, Switzerland"
            SGRef: number: 3; type: "Journal Article"
FEATURES             Location/Qualifiers
     source          1..5936
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     misc_feature    56..711
                     /note="fragment of the pyrFE genes from Staphylococcus
                     aureus; replacing the pC194 origin of replication"
     CDS             1002..1733
                     /gene="ermC"
                     /label="rRNA adenine N-6-methyltransferase"
                     /note="rRNA adenine N-6-methyltransferase from
                     Staphylococcus aureus. Accession#: P02979"
     misc_feature    complement(1867..1974)
                     /label="MCS"
                     /note="pBluescript multiple cloning site"
     promoter        complement(1983..2001)
                     /label="T7 promoter"
                     /note="promoter for bacteriophage T7 RNA polymerase"
     rep_origin      2450..2672
                     /label="pSC101 ori"
                     /note="low-copy replication origin that requires the Rep101
                     protein"
     CDS             2720..3667
                     /label="Rep101"
                     /note="RepA protein needed for replication with the pSC101
                     origin"
     CDS             4922..5710
                     /label="SmR"
                     /note="aminoglycoside adenylyltransferase (Murphy, 1985)"
ORIGIN
        1 ggcgcgccag atcgcgtgca taattcaaca gctaacctcc caataactac gcgtccatgg
       61 tacagttggt aaggcgatgg aaggactagc taaattgaat gttgatctgg taaatgttca
      121 tgctgctggt ggcgtaaaaa tgatgtctga ggccattaaa ggattaagaa aacataatca
      181 acatacaaaa attattgcag taacacagct tacgtcaaca acagaagaca tgttacgaca
      241 cgaacaaaat atacaaacat cgattgaaga ggccgtttta aattatgcca agttagcaaa
      301 tgcagctggt ttagatggcg ttgtttgttc acctcttgaa agtcgtatgt tgactgaaaa
      361 gttaggtaca tcatttttaa aagtaacacc aggtattaga cctaaaggtg catctcaaga
      421 tgaccaacac cgtattacga caccggaaga agcaagacag cttggttcga cgcatattgt
      481 agtcggtaga ccgattacac aaagtgacaa tccagtcgaa agttatcata aaattaaaga
      541 aagttggtta gtataatggc taaagaaatt gcaaaatcat tattagatat tgaagctgta
      601 acattatcac caaatgattt atatacatgg agttcaggta ttaaatcacc gatttactgt
      661 gataaccgtg ttacgttagg ttatccttta gttcgaggcg caatccgcga tgtacattta
      721 agaactttct ttttttacaa gaaaaaagaa attagataaa tctctcatat cttttattca
      781 ataatcgcat ccgattgcag tataaattta acgatcactc atcatgttca ttttatcaga
      841 gcttgtgcta taattatact aattttataa ggaggaaaaa atatgggcat ttttagtatt
      901 tttgtaatca gcacagttca ttatcaacca aacaaaaaat aagtggttat aatgaatcgt
      961 taataagcaa aattcatata accaaattaa agagggttat aatgaacgag aaaaatataa
     1021 aacacagtca aaactttatt acttcaaaac ataatataga taaaataatg acaaatataa
     1081 gattaaatga acatgataat atctttgaaa tcggctcagg aaaaggccat tttacccttg
     1141 aattagtaaa gaggtgtaat ttcgtaactg ccattgaaat agaccataaa ttatgcaaaa
     1201 ctacagaaaa taaacttgtt gatcacgata atttccaagt tttaaacaag gatatattgc
     1261 agtttaaatt tcctaaaaac caatcctata aaatatatgg taatatacct tataacataa
     1321 gtacggatat aatacgcaaa attgtttttg atagtatagc taatgagatt tatttaatcg
     1381 tggaatacgg gtttgctaaa agattattaa atacaaaacg ctcattggca ttacttttaa
     1441 tggcagaagt tgatatttct atattaagta tggttccaag agaatatttt catcctaaac
     1501 ctaaagtgaa tagctcactt atcagattaa gtaggaaaaa atcaagaata tcacacaaag
     1561 ataaacaaaa gtataattat ttcgttatga aatgggttaa caaagaatac aagaaaatat
     1621 ttacaaaaaa tcaatttaac aattccttaa aacatgcagg aattgacgat ttaaacaata
     1681 ttagctttga acaattctta tctcttttca atagctataa attatttaat aagtaagtta
     1741 agggatgcat aaactgcatc ccttaacttg tttttcgtgt gcctattttt tgtgttcgaa
     1801 tgccccgtta gttgaagaag gtttttatat tacagctcca gatctcacta aagggaacaa
     1861 aagctgggta ccgggccccc cctcgaggtc gacggtatcg ataagcttga tatcgaattc
     1921 ctgcagcccg ggggatccac tagttctaga gcggccgcca ccgcggtgga gctccaattc
     1981 gccctatagt gagtcgtatt aggcgcgcct tactgggtgc attagccagt ctgaatgacc
     2041 tgtcacggga taatccgaag tggtcagact ggaaaatcag agggcaggaa ctgctgaaca
     2101 gcaaaaagtc agatagcacc acatagcaga cccgccataa aacgccctga gaagcccgtg
     2161 acgggctttt cttgtattat gggtagtttc cttgcatgaa tccataaaag gcgcctgtag
     2221 tgccatttac ccccattcac tgccagagcc gtgagcgcag cgaactgaat gtcacgaaaa
     2281 agacagcgac tcaggtgcct gatggtcgga gacaaaagga atattcagcg atttgcccga
     2341 gcttgcgagg gtgctactta agcctttagg gttttaaggt ctgttttgta gaggagcaaa
     2401 cagcgtttgc gacatccttt tgtaatactg cggaactgac taaagtagtg agttatacac
     2461 agggctggga tctattcttt ttatcttttt ttattctttc tttattctat aaattataac
     2521 cacttgaata taaacaaaaa aaacacacaa aggtctagcg gaatttacag agggtctagc
     2581 agaatttaca agttttccag caaaggtcta gcagaattta cagataccca caactcaaag
     2641 gaaaaggact agtaattatc attgactagc ccatctcaat tggtatagtg attaaaatca
     2701 cctagaccaa ttgagatgta tgtctgaatt agttgttttc aaagcaaatg aactagcgat
     2761 tagtcgctat gacttaacgg agcatgaaac caagctaatt ttatgctgtg tggcactact
     2821 caaccccacg attgaaaacc ctacaaggaa agaacggacg gtatcgttca cttataacca
     2881 atacgctcag atgatgaaca tcagtaggga aaatgcttat ggtgtattag ctaaagcaac
     2941 cagagagctg atgacgagaa ctgtggaaat caggaatcct ttggttaaag gctttaagat
     3001 tttccagtgg acaaactatg ccaagttctc aagcgaaaaa ttagaattag tttttagtga
     3061 agagatattg ccttatcttt tccagttaaa aaaattcata aaatataatc tggaacatgt
     3121 taagtctttt gaaaacaaat actctatgag gatttatgag tggttattaa aagaactaac
     3181 acaaaagaaa actcacaagg caaatataga gattagcctt gatgaattta agttcatgtt
     3241 aatgcttgaa aataactacc atgagtttaa aaggcttaac caatgggttt tgaaaccaat
     3301 aagtaaagat ttaaacactt acagcaatat gaaattggtg gttgataagc gaggccgccc
     3361 gactgatacg ttgattttcc aagttgaact agatagacaa atggatctcg taaccgaact
     3421 tgagaacaac cagataaaaa tgaatggtga caaaatacca acaaccatta catcagattc
     3481 ctacctacgt aacggactaa gaaaaacact acacgatgct ttaactgcaa aaattcagct
     3541 caccagtttt gaggcaaaat ttttgagtga catgcaaagt aagtatgatc tcaatggttc
     3601 gttctcatgg ctcacgcaaa aacaacgaac cacactagag aacatactgg ctaaatacgg
     3661 aaggatctga ggttcttatg gctcttgtat ctatcagtga agcatcaaga ctaacaaaca
     3721 aaagtagaac aactgttcac cgttacatat caaagggaaa actgtccata tgcacagatg
     3781 aaaacggtgt aaaaaagata gatacatcag agcttttacg agtttttggt gcattcaaag
     3841 ctgttcacca tgaacagatc gacaatgtaa cagatgaaca gcatgtaaca cctaatagaa
     3901 caggtgaaac cagtaaaaca aagcaactag aacatgaaat tgaacacctg agacaacttg
     3961 ttacagctca acagtcacac atagacagcc tgaaacaggc gatgctgctt atcgaatcaa
     4021 agctgccgac aacacgggag ccagtgacgc ctcccgtggg gaaaaaatca tggcaattct
     4081 ggaagaaata gcgctttcag ccggcaaacc ggctgaagcc ggatctgcga ttctgataac
     4141 aaactagcaa caccagaaca gcccgtttgc gggcagcaaa acccgtggga attaattccc
     4201 ctgctcgcgc aggctgggtg ccaagctctc gggtaacatc aaggcccgat ccttggagcc
     4261 cttgccctcc cgcacgatga tcgtgccgtg atcgaaatcc agatccttga cccgcagttg
     4321 caaaccctca ctgatccgca tgcccgttcc atacagaagc tgggcgaaca aacgatgctc
     4381 gccttccaga aaaccgagga tgcgaaccac ttcatccggg gtcagcacca ccggcaagcg
     4441 ccgcgacggc cgaggtcttc cgatctcctg aagccagggc agatccgtgc acagcacctt
     4501 gccgtagaag aacagcaagg ccgccaatgc ctgacgatgc gtggagaccg aaaccttgcg
     4561 ctcgttcgcc agccaggaca gaaatgcctc gacttcgctg ctgcccaagg ttgccgggtg
     4621 acgcacaccg tggaaacgga tgaaggcacg aacccagtgg acataagcct gttcggttcg
     4681 taagctgtaa tgcaagtagc gtatgcgctc acgcaactgg tccagaacct tgaccgaacg
     4741 cagcggtggt aacggcgcag tggcggtttt catggcttgt tatgactgtt tttttggggt
     4801 acagtctatg cctcgggcat ccaagcagca agcgcgttac gccgtgggtc gatgtttgat
     4861 gttatggagc agcaacgatg ttacgcagca gggcagtcgc cctaaaacaa agttaaacat
     4921 catgagggaa gcggtgatcg ccgaagtatc gactcaacta tcagaggtag ttggcgtcat
     4981 cgagcgccat ctcgaaccga cgttgctggc cgtacatttg tacggctccg cagtggatgg
     5041 cggcctgaag ccacacagtg atattgattt gctggttacg gtgaccgtaa ggcttgatga
     5101 aacaacgcgg cgagctttga tcaacgacct tttggaaact tcggcttccc ctggagagag
     5161 cgagattctc cgcgctgtag aagtcaccat tgttgtgcac gacgacatca ttccgtggcg
     5221 ttatccagct aagcgcgaac tgcaatttgg agaatggcag cgcaatgaca ttcttgcagg
     5281 tatcttcgag ccagccacga tcgacattga tctggctatc ttgctgacaa aagcaagaga
     5341 acatagcgtt gccttggtag gtccagcggc ggaggaactc tttgatccgg ttcctgaaca
     5401 ggatctattt gaggcgctaa atgaaacctt aacgctatgg aactcgccgc ccgactgggc
     5461 tggcgatgag cgaaatgtag tgcttacgtt gtcccgcatt tggtacagcg cagtaaccgg
     5521 caaaatcgcg ccgaaggatg tcgctgccga ctgggcaatg gagcgcctgc cggcccagta
     5581 tcagcccgtc atacttgaag ctagacaggc ttatcttgga caagaagaag atcgcttggc
     5641 ctcgcgcgca gatcagttgg aagaatttgt ccactacgtg aaaggcgaga tcaccaaggt
     5701 agtcggcaaa taatgtctaa caattcgttc aagccgacgc cgcttcgcgg cgcggcttaa
     5761 ctcaagcgtt agatgcacta agcacataat tgctcacagc caaactatca ggtcaagtct
     5821 gcttttatta tttttaagcg tgcataataa gccctacaca aattgggaga tatatcatga
     5881 aaggctggct ttttcttgtt atcgcaatag ttggcgaagt aatcgcaaca tccgca
//
