LOCUS       V003702                 6793 bp    DNA     circular SYN 18-DEC-2018
DEFINITION  Exported.
ACCESSION   V003702
VERSION     V003702
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
            .
REFERENCE   1  (bases 1 to 6793)
  AUTHORS   Redder P, Linder P.
  TITLE     New Range of Vectors with a Stringent 5-Fluoroorotic Acid-Based
            Counterselection System for Generating Mutants by Allelic
            Replacement in Staphylococcus aureus
  JOURNAL   Appl. Environ. Microbiol. 78 (11), 3846-3854 (2012)
   PUBMED   22447609
REFERENCE   2  (bases 1 to 6793)
  AUTHORS   Redder P, Linder P.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2012) Department of Microbiology and Molecular
            Medicine, University of Geneva, Faculty of Medicine, Rue
            Michel-Servet 1, Geneva 1203, Switzerland
REFERENCE   3  (bases 1 to 6793)
  TITLE     Direct Submission
REFERENCE   4  (bases 1 to 6793)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     ##Assembly-Data-START##
            Assembly Method       :: BioEdit, CAP v. 7.0.9.0
            Sequencing Technology :: Sanger dideoxy sequencing
            ##Assembly-Data-END##
            SGRef: number: 1; type: "Journal Article"; journalName: "Appl.
            Environ. Microbiol."; date: "2012"; volume: "78"; issue: "11";
            pages: "3846-3854"
            SGRef: number: 2; type: "Journal Article"; journalName: "Submitted
            (12-MAR-2012) Department of Microbiology and Molecular Medicine,
            University of Geneva, Faculty of Medicine, Rue Michel-Servet 1,
            Geneva 1203, Switzerland"
            SGRef: number: 3; type: "Journal Article"
FEATURES             Location/Qualifiers
     source          1..6793
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     CDS             1609..2340
                     /gene="ermC"
                     /label="rRNA adenine N-6-methyltransferase"
                     /note="rRNA adenine N-6-methyltransferase from
                     Staphylococcus aureus. Accession#: P02979"
     misc_feature    2479..2723
                     /note="derived from pLP763; Pprot promoter"
     misc_feature    complement(2724..2831)
                     /label="MCS"
                     /note="pBluescript multiple cloning site"
     promoter        complement(2840..2858)
                     /label="T7 promoter"
                     /note="promoter for bacteriophage T7 RNA polymerase"
     rep_origin      3307..3529
                     /label="pSC101 ori"
                     /note="low-copy replication origin that requires the Rep101
                     protein"
     CDS             3577..4524
                     /label="Rep101"
                     /note="RepA protein needed for replication with the pSC101
                     origin"
     CDS             5779..6567
                     /label="SmR"
                     /note="aminoglycoside adenylyltransferase (Murphy, 1985)"
ORIGIN
        1 ggcgcgccag atcgcgtgca taattcaaca gctaacctcc caataactac atggtgttat
       61 cgggaggtca gctgttagca cttatatttt gttattgttc ttcctcgatt tcgtctatca
      121 ttttgtgatt aatttctctt ttttcttgtt ctgttaagtc ataaagttca ctagctaaat
      181 actctttttg tttccaaata taaaaaattt gatagatata ttcggttgga tcaatttctt
      241 ttaagtaatc taaatcccca ttttttaatt tctttttagc ctctttaaat aatcctgaat
      301 aaactaatac ctgtttacct ttaagtgatt tataaaatgc atcaaagact ttttgattta
      361 ttaaataatc actatcttta ccagaatact tagccatttc atataattct ttattattat
      421 tttgtcttat tttttgaact tgaacttgtg ttatttctga aatgcccgtt acatcacgcc
      481 ataaatctaa ccattcttgt tggctaatat aatatctttt atctgtgaaa tacgatttat
      541 ttactgcaat taacacatga aaatgaggat tataatcatc tcttttttta ttatatgtaa
      601 tctctaactt acgaacatat ccctttataa cactacctac tttttttctc tttataagtt
      661 ttctaaaaga attattataa cgttttattt cattttctaa ttcatcactc attacattag
      721 gtgtagtcaa agttaaaaag ataaactcct ttttctcttg ctgcttaata tattgcatca
      781 tcaaagataa acccaatgca tcttttctag cttttctcca agcacagaca ggacaaaatc
      841 gatttttaca agaattagct ttatataatt tctgtttttc taaagtttta tcagctacaa
      901 aagacagaaa tgtattgcaa tcttcaacta aatccatttg attctctcca atatgacgtt
      961 taataaattt ctgaaatact tgatttcttt gttttttctc agtatacttt tccatgttat
     1021 aacacataaa aacaacttag ttttcacaaa ctatgacaat aaaaaaagtt gctttttccc
     1081 ctttctatgt atgtttttta ctagtcattt aaaacgatac attaataggt acgaaaaagc
     1141 aacttttttt gcgcttaaaa ccagtcatac caataactta agggtaacta gcctcgccgg
     1201 caatagttac ccttattatc aagataagaa agaaaaggat ttttcgctac gctcaaatcc
     1261 tttaaaaaaa cacaaaagac cacatttttt aatgtggtct tttattcttc aactaaatgt
     1321 acatttaaga actttctttt tttacaagaa aaaagaaatt agataaatct ctcatatctt
     1381 ttattcaata atcgcatccg attgcagtat aaatttaacg atcactcatc atgttcattt
     1441 tatcagagct tgtgctataa ttatactaat tttataagga ggaaaaaata tgggcatttt
     1501 tagtattttt gtaatcagca cagttcatta tcaaccaaac aaaaaataag tggttataat
     1561 gaatcgttaa taagcaaaat tcatataacc aaattaaaga gggttataat gaacgagaaa
     1621 aatataaaac acagtcaaaa ctttattact tcaaaacata atatagataa aataatgaca
     1681 aatataagat taaatgaaca tgataatatc tttgaaatcg gctcaggaaa aggccatttt
     1741 acccttgaat tagtaaagag gtgtaatttc gtaactgcca ttgaaataga ccataaatta
     1801 tgcaaaacta cagaaaataa acttgttgat cacgataatt tccaagtttt aaacaaggat
     1861 atattgcagt ttaaatttcc taaaaaccaa tcctataaaa tatatggtaa tataccttat
     1921 aacataagta cggatataat acgcaaaatt gtttttgata gtatagctaa tgagatttat
     1981 ttaatcgtgg aatacgggtt tgctaaaaga ttattaaata caaaacgctc attggcatta
     2041 cttttaatgg cagaagttga tatttctata ttaagtatgg ttccaagaga atattttcat
     2101 cctaaaccta aagtgaatag ctcacttatc agattaagta ggaaaaaatc aagaatatca
     2161 cacaaagata aacaaaagta taattatttc gttatgaaat gggttaacaa agaatacaag
     2221 aaaatattta caaaaaatca atttaacaat tccttaaaac atgcaggaat tgacgattta
     2281 aacaatatta gctttgaaca attcttatct cttttcaata gctataaatt atttaataag
     2341 taagttaagg gatgcataaa ctgcatccct taacttgttt ttcgtgtgcc tattttttgt
     2401 gttcgaatgc cccgttagtt gaagaaggtt tttatattac agctccagat ctcactaaag
     2461 ggaacaaaag ctgggtacaa atactcattt tctaataatg ctaaaaattt caaaacatct
     2521 atagtctgta aacggctaaa taataacgct aaaagttaat ttacagataa aaaaattaat
     2581 agaagattaa aattttcgtt gaatttgttc ttcaatagta tataatataa tagtatataa
     2641 tattatataa tataatctta actacatcaa gcgtagggtt tgatttggtt atgaaacttt
     2701 tggaaagtgg aggatattgg atgggtaccg ggccccccct cgaggtcgac ggtatcgata
     2761 agcttgatat cgaattcctg cagcccgggg gatccactag ttctagagcg gccgccaccg
     2821 cggtggagct ccaattcgcc ctatagtgag tcgtattagg cgcgccttac tgggtgcatt
     2881 agccagtctg aatgacctgt cacgggataa tccgaagtgg tcagactgga aaatcagagg
     2941 gcaggaactg ctgaacagca aaaagtcaga tagcaccaca tagcagaccc gccataaaac
     3001 gccctgagaa gcccgtgacg ggcttttctt gtattatggg tagtttcctt gcatgaatcc
     3061 ataaaaggcg cctgtagtgc catttacccc cattcactgc cagagccgtg agcgcagcga
     3121 actgaatgtc acgaaaaaga cagcgactca ggtgcctgat ggtcggagac aaaaggaata
     3181 ttcagcgatt tgcccgagct tgcgagggtg ctacttaagc ctttagggtt ttaaggtctg
     3241 ttttgtagag gagcaaacag cgtttgcgac atccttttgt aatactgcgg aactgactaa
     3301 agtagtgagt tatacacagg gctgggatct attcttttta tcttttttta ttctttcttt
     3361 attctataaa ttataaccac ttgaatataa acaaaaaaaa cacacaaagg tctagcggaa
     3421 tttacagagg gtctagcaga atttacaagt tttccagcaa aggtctagca gaatttacag
     3481 atacccacaa ctcaaaggaa aaggactagt aattatcatt gactagccca tctcaattgg
     3541 tatagtgatt aaaatcacct agaccaattg agatgtatgt ctgaattagt tgttttcaaa
     3601 gcaaatgaac tagcgattag tcgctatgac ttaacggagc atgaaaccaa gctaatttta
     3661 tgctgtgtgg cactactcaa ccccacgatt gaaaacccta caaggaaaga acggacggta
     3721 tcgttcactt ataaccaata cgctcagatg atgaacatca gtagggaaaa tgcttatggt
     3781 gtattagcta aagcaaccag agagctgatg acgagaactg tggaaatcag gaatcctttg
     3841 gttaaaggct ttaagatttt ccagtggaca aactatgcca agttctcaag cgaaaaatta
     3901 gaattagttt ttagtgaaga gatattgcct tatcttttcc agttaaaaaa attcataaaa
     3961 tataatctgg aacatgttaa gtcttttgaa aacaaatact ctatgaggat ttatgagtgg
     4021 ttattaaaag aactaacaca aaagaaaact cacaaggcaa atatagagat tagccttgat
     4081 gaatttaagt tcatgttaat gcttgaaaat aactaccatg agtttaaaag gcttaaccaa
     4141 tgggttttga aaccaataag taaagattta aacacttaca gcaatatgaa attggtggtt
     4201 gataagcgag gccgcccgac tgatacgttg attttccaag ttgaactaga tagacaaatg
     4261 gatctcgtaa ccgaacttga gaacaaccag ataaaaatga atggtgacaa aataccaaca
     4321 accattacat cagattccta cctacgtaac ggactaagaa aaacactaca cgatgcttta
     4381 actgcaaaaa ttcagctcac cagttttgag gcaaaatttt tgagtgacat gcaaagtaag
     4441 tatgatctca atggttcgtt ctcatggctc acgcaaaaac aacgaaccac actagagaac
     4501 atactggcta aatacggaag gatctgaggt tcttatggct cttgtatcta tcagtgaagc
     4561 atcaagacta acaaacaaaa gtagaacaac tgttcaccgt tacatatcaa agggaaaact
     4621 gtccatatgc acagatgaaa acggtgtaaa aaagatagat acatcagagc ttttacgagt
     4681 ttttggtgca ttcaaagctg ttcaccatga acagatcgac aatgtaacag atgaacagca
     4741 tgtaacacct aatagaacag gtgaaaccag taaaacaaag caactagaac atgaaattga
     4801 acacctgaga caacttgtta cagctcaaca gtcacacata gacagcctga aacaggcgat
     4861 gctgcttatc gaatcaaagc tgccgacaac acgggagcca gtgacgcctc ccgtggggaa
     4921 aaaatcatgg caattctgga agaaatagcg ctttcagccg gcaaaccggc tgaagccgga
     4981 tctgcgattc tgataacaaa ctagcaacac cagaacagcc cgtttgcggg cagcaaaacc
     5041 cgtgggaatt aattcccctg ctcgcgcagg ctgggtgcca agctctcggg taacatcaag
     5101 gcccgatcct tggagccctt gccctcccgc acgatgatcg tgccgtgatc gaaatccaga
     5161 tccttgaccc gcagttgcaa accctcactg atccgcatgc ccgttccata cagaagctgg
     5221 gcgaacaaac gatgctcgcc ttccagaaaa ccgaggatgc gaaccacttc atccggggtc
     5281 agcaccaccg gcaagcgccg cgacggccga ggtcttccga tctcctgaag ccagggcaga
     5341 tccgtgcaca gcaccttgcc gtagaagaac agcaaggccg ccaatgcctg acgatgcgtg
     5401 gagaccgaaa ccttgcgctc gttcgccagc caggacagaa atgcctcgac ttcgctgctg
     5461 cccaaggttg ccgggtgacg cacaccgtgg aaacggatga aggcacgaac ccagtggaca
     5521 taagcctgtt cggttcgtaa gctgtaatgc aagtagcgta tgcgctcacg caactggtcc
     5581 agaaccttga ccgaacgcag cggtggtaac ggcgcagtgg cggttttcat ggcttgttat
     5641 gactgttttt ttggggtaca gtctatgcct cgggcatcca agcagcaagc gcgttacgcc
     5701 gtgggtcgat gtttgatgtt atggagcagc aacgatgtta cgcagcaggg cagtcgccct
     5761 aaaacaaagt taaacatcat gagggaagcg gtgatcgccg aagtatcgac tcaactatca
     5821 gaggtagttg gcgtcatcga gcgccatctc gaaccgacgt tgctggccgt acatttgtac
     5881 ggctccgcag tggatggcgg cctgaagcca cacagtgata ttgatttgct ggttacggtg
     5941 accgtaaggc ttgatgaaac aacgcggcga gctttgatca acgacctttt ggaaacttcg
     6001 gcttcccctg gagagagcga gattctccgc gctgtagaag tcaccattgt tgtgcacgac
     6061 gacatcattc cgtggcgtta tccagctaag cgcgaactgc aatttggaga atggcagcgc
     6121 aatgacattc ttgcaggtat cttcgagcca gccacgatcg acattgatct ggctatcttg
     6181 ctgacaaaag caagagaaca tagcgttgcc ttggtaggtc cagcggcgga ggaactcttt
     6241 gatccggttc ctgaacagga tctatttgag gcgctaaatg aaaccttaac gctatggaac
     6301 tcgccgcccg actgggctgg cgatgagcga aatgtagtgc ttacgttgtc ccgcatttgg
     6361 tacagcgcag taaccggcaa aatcgcgccg aaggatgtcg ctgccgactg ggcaatggag
     6421 cgcctgccgg cccagtatca gcccgtcata cttgaagcta gacaggctta tcttggacaa
     6481 gaagaagatc gcttggcctc gcgcgcagat cagttggaag aatttgtcca ctacgtgaaa
     6541 ggcgagatca ccaaggtagt cggcaaataa tgtctaacaa ttcgttcaag ccgacgccgc
     6601 ttcgcggcgc ggcttaactc aagcgttaga tgcactaagc acataattgc tcacagccaa
     6661 actatcaggt caagtctgct tttattattt ttaagcgtgc ataataagcc ctacacaaat
     6721 tgggagatat atcatgaaag gctggctttt tcttgttatc gcaatagttg gcgaagtaat
     6781 cgcaacatcc gca
//
