LOCUS       V003703                 6543 bp    DNA     circular SYN 18-DEC-2018
DEFINITION  Exported.
ACCESSION   V003703
VERSION     V003703
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
            .
REFERENCE   1  (bases 1 to 6543)
  AUTHORS   Redder P, Linder P.
  TITLE     New Range of Vectors with a Stringent 5-Fluoroorotic Acid-Based
            Counterselection System for Generating Mutants by Allelic
            Replacement in Staphylococcus aureus
  JOURNAL   Appl. Environ. Microbiol. 78 (11), 3846-3854 (2012)
   PUBMED   22447609
REFERENCE   2  (bases 1 to 6543)
  AUTHORS   Redder P, Linder P.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2012) Department of Microbiology and Molecular
            Medicine, University of Geneva, Faculty of Medicine, Rue
            Michel-Servet 1, Geneva 1203, Switzerland
REFERENCE   3  (bases 1 to 6543)
  TITLE     Direct Submission
REFERENCE   4  (bases 1 to 6543)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     ##Assembly-Data-START##
            Assembly Method       :: BioEdit, CAP v. 7.0.9.0
            Sequencing Technology :: Sanger dideoxy sequencing
            ##Assembly-Data-END##
            SGRef: number: 1; type: "Journal Article"; journalName: "Appl.
            Environ. Microbiol."; date: "2012"; volume: "78"; issue: "11";
            pages: "3846-3854"
            SGRef: number: 2; type: "Journal Article"; journalName: "Submitted
            (12-MAR-2012) Department of Microbiology and Molecular Medicine,
            University of Geneva, Faculty of Medicine, Rue Michel-Servet 1,
            Geneva 1203, Switzerland"
            SGRef: number: 3; type: "Journal Article"
FEATURES             Location/Qualifiers
     source          1..6543
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     CDS             1609..2340
                     /gene="ermC"
                     /label="rRNA adenine N-6-methyltransferase"
                     /note="rRNA adenine N-6-methyltransferase from
                     Staphylococcus aureus. Accession#: P02979"
     misc_feature    complement(2474..2581)
                     /label="MCS"
                     /note="pBluescript multiple cloning site"
     promoter        complement(2590..2608)
                     /label="T7 promoter"
                     /note="promoter for bacteriophage T7 RNA polymerase"
     rep_origin      3057..3279
                     /label="pSC101 ori"
                     /note="low-copy replication origin that requires the Rep101
                     protein"
     CDS             3327..4274
                     /label="Rep101"
                     /note="RepA protein needed for replication with the pSC101
                     origin"
     CDS             5529..6317
                     /label="SmR"
                     /note="aminoglycoside adenylyltransferase (Murphy, 1985)"
ORIGIN
        1 ggcgcgccag atcgcgtgca taattcaaca gctaacctcc caataactac atggtgttat
       61 cgggaggtca gctgttagca cttatatttt gttattgttc ttcctcgatt tcgtctatca
      121 ttttgtgatt aatttctctt ttttcttgtt ctgttaagtc ataaagttca ctagctaaat
      181 actctttttg tttccaaata taaaaaattt gatagatata ttcggttgga tcaatttctt
      241 ttaagtaatc taaatcccca ttttttaatt tctttttagc ctctttaaat aatcctgaat
      301 aaactaatac ctgtttacct ttaagtgatt tataaaatgc atcaaagact ttttgattta
      361 ttaaataatc actatcttta ccagaatact tagccatttc atataattct ttattattat
      421 tttgtcttat tttttgaact tgaacttgtg ttatttctga aatgcccgtt acatcacgcc
      481 ataaatctaa ccattcttgt tggctaatat aatatctttt atctgtgaaa tacgatttat
      541 ttactgcaat taacacatga aaatgaggat tataatcatc tcttttttta ttatatgtaa
      601 tctctaactt acgaacatat ccctttataa cactacctac tttttttctc tttataagtt
      661 ttctaaaaga attattataa cgttttattt cattttctaa ttcatcactc attacattag
      721 gtgtagtcaa agttaaaaag ataaactcct ttttctcttg ctgcttaata tattgcatca
      781 tcaaagataa acccaatgca tcttttctag cttttctcca agcacagaca ggacaaaatc
      841 gatttttaca agaattagct ttatataatt tctgtttttc taaagtttta tcagctacaa
      901 aagacagaaa tgtattgcaa tcttcaacta aatccatttg attctctcca atatgacgtt
      961 taataaattt ctgaaatact tgatttcttt gttttttctc agtatacttt tccatgttat
     1021 aacacataaa aacaacttag ttttcacaaa ctatgacaat aaaaaaagtt gctttttccc
     1081 ctttctatgt atgtttttta ctagtcattt aaaacgatac attaataggt acgaaaaagc
     1141 aacttttttt gcgcttaaaa ccagtcatac caataactta agggtaacta gcctcgccgg
     1201 caatagttac ccttattatc aagataagaa agaaaaggat ttttcgctac gctcaaatcc
     1261 tttaaaaaaa cacaaaagac cacatttttt aatgtggtct tttattcttc aactaaatgt
     1321 acatttaaga actttctttt tttacaagaa aaaagaaatt agataaatct ctcatatctt
     1381 ttattcaata atcgcatccg attgcagtat aaatttaacg atcactcatc atgttcattt
     1441 tatcagagct tgtgctataa ttatactaat tttataagga ggaaaaaata tgggcatttt
     1501 tagtattttt gtaatcagca cagttcatta tcaaccaaac aaaaaataag tggttataat
     1561 gaatcgttaa taagcaaaat tcatataacc aaattaaaga gggttataat gaacgagaaa
     1621 aatataaaac acagtcaaaa ctttattact tcaaaacata atatagataa aataatgaca
     1681 aatataagat taaatgaaca tgataatatc tttgaaatcg gctcaggaaa aggccatttt
     1741 acccttgaat tagtaaagag gtgtaatttc gtaactgcca ttgaaataga ccataaatta
     1801 tgcaaaacta cagaaaataa acttgttgat cacgataatt tccaagtttt aaacaaggat
     1861 atattgcagt ttaaatttcc taaaaaccaa tcctataaaa tatatggtaa tataccttat
     1921 aacataagta cggatataat acgcaaaatt gtttttgata gtatagctaa tgagatttat
     1981 ttaatcgtgg aatacgggtt tgctaaaaga ttattaaata caaaacgctc attggcatta
     2041 cttttaatgg cagaagttga tatttctata ttaagtatgg ttccaagaga atattttcat
     2101 cctaaaccta aagtgaatag ctcacttatc agattaagta ggaaaaaatc aagaatatca
     2161 cacaaagata aacaaaagta taattatttc gttatgaaat gggttaacaa agaatacaag
     2221 aaaatattta caaaaaatca atttaacaat tccttaaaac atgcaggaat tgacgattta
     2281 aacaatatta gctttgaaca attcttatct cttttcaata gctataaatt atttaataag
     2341 taagttaagg gatgcataaa ctgcatccct taacttgttt ttcgtgtgcc tattttttgt
     2401 gttcgaatgc cccgttagtt gaagaaggtt tttatattac agctccagat ctcactaaag
     2461 ggaacaaaag ctgggtaccg ggccccccct cgaggtcgac ggtatcgata agcttgatat
     2521 cgaattcctg cagcccgggg gatccactag ttctagagcg gccgccaccg cggtggagct
     2581 ccaattcgcc ctatagtgag tcgtattagg cgcgccttac tgggtgcatt agccagtctg
     2641 aatgacctgt cacgggataa tccgaagtgg tcagactgga aaatcagagg gcaggaactg
     2701 ctgaacagca aaaagtcaga tagcaccaca tagcagaccc gccataaaac gccctgagaa
     2761 gcccgtgacg ggcttttctt gtattatggg tagtttcctt gcatgaatcc ataaaaggcg
     2821 cctgtagtgc catttacccc cattcactgc cagagccgtg agcgcagcga actgaatgtc
     2881 acgaaaaaga cagcgactca ggtgcctgat ggtcggagac aaaaggaata ttcagcgatt
     2941 tgcccgagct tgcgagggtg ctacttaagc ctttagggtt ttaaggtctg ttttgtagag
     3001 gagcaaacag cgtttgcgac atccttttgt aatactgcgg aactgactaa agtagtgagt
     3061 tatacacagg gctgggatct attcttttta tcttttttta ttctttcttt attctataaa
     3121 ttataaccac ttgaatataa acaaaaaaaa cacacaaagg tctagcggaa tttacagagg
     3181 gtctagcaga atttacaagt tttccagcaa aggtctagca gaatttacag atacccacaa
     3241 ctcaaaggaa aaggactagt aattatcatt gactagccca tctcaattgg tatagtgatt
     3301 aaaatcacct agaccaattg agatgtatgt ctgaattagt tgttttcaaa gcaaatgaac
     3361 tagcgattag tcgctatgac ttaacggagc atgaaaccaa gctaatttta tgctgtgtgg
     3421 cactactcaa ccccacgatt gaaaacccta caaggaaaga acggacggta tcgttcactt
     3481 ataaccaata cgctcagatg atgaacatca gtagggaaaa tgcttatggt gtattagcta
     3541 aagcaaccag agagctgatg acgagaactg tggaaatcag gaatcctttg gttaaaggct
     3601 ttaagatttt ccagtggaca aactatgcca agttctcaag cgaaaaatta gaattagttt
     3661 ttagtgaaga gatattgcct tatcttttcc agttaaaaaa attcataaaa tataatctgg
     3721 aacatgttaa gtcttttgaa aacaaatact ctatgaggat ttatgagtgg ttattaaaag
     3781 aactaacaca aaagaaaact cacaaggcaa atatagagat tagccttgat gaatttaagt
     3841 tcatgttaat gcttgaaaat aactaccatg agtttaaaag gcttaaccaa tgggttttga
     3901 aaccaataag taaagattta aacacttaca gcaatatgaa attggtggtt gataagcgag
     3961 gccgcccgac tgatacgttg attttccaag ttgaactaga tagacaaatg gatctcgtaa
     4021 ccgaacttga gaacaaccag ataaaaatga atggtgacaa aataccaaca accattacat
     4081 cagattccta cctacgtaac ggactaagaa aaacactaca cgatgcttta actgcaaaaa
     4141 ttcagctcac cagttttgag gcaaaatttt tgagtgacat gcaaagtaag tatgatctca
     4201 atggttcgtt ctcatggctc acgcaaaaac aacgaaccac actagagaac atactggcta
     4261 aatacggaag gatctgaggt tcttatggct cttgtatcta tcagtgaagc atcaagacta
     4321 acaaacaaaa gtagaacaac tgttcaccgt tacatatcaa agggaaaact gtccatatgc
     4381 acagatgaaa acggtgtaaa aaagatagat acatcagagc ttttacgagt ttttggtgca
     4441 ttcaaagctg ttcaccatga acagatcgac aatgtaacag atgaacagca tgtaacacct
     4501 aatagaacag gtgaaaccag taaaacaaag caactagaac atgaaattga acacctgaga
     4561 caacttgtta cagctcaaca gtcacacata gacagcctga aacaggcgat gctgcttatc
     4621 gaatcaaagc tgccgacaac acgggagcca gtgacgcctc ccgtggggaa aaaatcatgg
     4681 caattctgga agaaatagcg ctttcagccg gcaaaccggc tgaagccgga tctgcgattc
     4741 tgataacaaa ctagcaacac cagaacagcc cgtttgcggg cagcaaaacc cgtgggaatt
     4801 aattcccctg ctcgcgcagg ctgggtgcca agctctcggg taacatcaag gcccgatcct
     4861 tggagccctt gccctcccgc acgatgatcg tgccgtgatc gaaatccaga tccttgaccc
     4921 gcagttgcaa accctcactg atccgcatgc ccgttccata cagaagctgg gcgaacaaac
     4981 gatgctcgcc ttccagaaaa ccgaggatgc gaaccacttc atccggggtc agcaccaccg
     5041 gcaagcgccg cgacggccga ggtcttccga tctcctgaag ccagggcaga tccgtgcaca
     5101 gcaccttgcc gtagaagaac agcaaggccg ccaatgcctg acgatgcgtg gagaccgaaa
     5161 ccttgcgctc gttcgccagc caggacagaa atgcctcgac ttcgctgctg cccaaggttg
     5221 ccgggtgacg cacaccgtgg aaacggatga aggcacgaac ccagtggaca taagcctgtt
     5281 cggttcgtaa gctgtaatgc aagtagcgta tgcgctcacg caactggtcc agaaccttga
     5341 ccgaacgcag cggtggtaac ggcgcagtgg cggttttcat ggcttgttat gactgttttt
     5401 ttggggtaca gtctatgcct cgggcatcca agcagcaagc gcgttacgcc gtgggtcgat
     5461 gtttgatgtt atggagcagc aacgatgtta cgcagcaggg cagtcgccct aaaacaaagt
     5521 taaacatcat gagggaagcg gtgatcgccg aagtatcgac tcaactatca gaggtagttg
     5581 gcgtcatcga gcgccatctc gaaccgacgt tgctggccgt acatttgtac ggctccgcag
     5641 tggatggcgg cctgaagcca cacagtgata ttgatttgct ggttacggtg accgtaaggc
     5701 ttgatgaaac aacgcggcga gctttgatca acgacctttt ggaaacttcg gcttcccctg
     5761 gagagagcga gattctccgc gctgtagaag tcaccattgt tgtgcacgac gacatcattc
     5821 cgtggcgtta tccagctaag cgcgaactgc aatttggaga atggcagcgc aatgacattc
     5881 ttgcaggtat cttcgagcca gccacgatcg acattgatct ggctatcttg ctgacaaaag
     5941 caagagaaca tagcgttgcc ttggtaggtc cagcggcgga ggaactcttt gatccggttc
     6001 ctgaacagga tctatttgag gcgctaaatg aaaccttaac gctatggaac tcgccgcccg
     6061 actgggctgg cgatgagcga aatgtagtgc ttacgttgtc ccgcatttgg tacagcgcag
     6121 taaccggcaa aatcgcgccg aaggatgtcg ctgccgactg ggcaatggag cgcctgccgg
     6181 cccagtatca gcccgtcata cttgaagcta gacaggctta tcttggacaa gaagaagatc
     6241 gcttggcctc gcgcgcagat cagttggaag aatttgtcca ctacgtgaaa ggcgagatca
     6301 ccaaggtagt cggcaaataa tgtctaacaa ttcgttcaag ccgacgccgc ttcgcggcgc
     6361 ggcttaactc aagcgttaga tgcactaagc acataattgc tcacagccaa actatcaggt
     6421 caagtctgct tttattattt ttaagcgtgc ataataagcc ctacacaaat tgggagatat
     6481 atcatgaaag gctggctttt tcttgttatc gcaatagttg gcgaagtaat cgcaacatcc
     6541 gca
//
