LOCUS       V003704                 7008 bp    DNA     circular SYN 18-DEC-2018
DEFINITION  Exported.
ACCESSION   V003704
VERSION     V003704
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
            .
REFERENCE   1  (bases 1 to 7008)
  AUTHORS   Redder P, Linder P.
  TITLE     New Range of Vectors with a Stringent 5-Fluoroorotic Acid-Based
            Counterselection System for Generating Mutants by Allelic
            Replacement in Staphylococcus aureus
  JOURNAL   Appl. Environ. Microbiol. 78 (11), 3846-3854 (2012)
   PUBMED   22447609
REFERENCE   2  (bases 1 to 7008)
  AUTHORS   Redder P, Linder P.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2012) Department of Microbiology and Molecular
            Medicine, University of Geneva, Faculty of Medicine, Rue
            Michel-Servet 1, Geneva 1203, Switzerland
REFERENCE   3  (bases 1 to 7008)
  TITLE     Direct Submission
REFERENCE   4  (bases 1 to 7008)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     ##Assembly-Data-START##
            Assembly Method       :: BioEdit, CAP v. 7.0.9.0
            Sequencing Technology :: Sanger dideoxy sequencing
            ##Assembly-Data-END##
            SGRef: number: 1; type: "Journal Article"; journalName: "Appl.
            Environ. Microbiol."; date: "2012"; volume: "78"; issue: "11";
            pages: "3846-3854"
            SGRef: number: 2; type: "Journal Article"; journalName: "Submitted
            (12-MAR-2012) Department of Microbiology and Molecular Medicine,
            University of Geneva, Faculty of Medicine, Rue Michel-Servet 1,
            Geneva 1203, Switzerland"
            SGRef: number: 3; type: "Journal Article"
FEATURES             Location/Qualifiers
     source          1..7008
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     rep_origin      complement(50..1209)
                     /direction=LEFT
                     /note="thermosensitive origin of replication; for
                     replication in Staphylococcus aureus; pE194ts"
     misc_feature    1210..1865
                     /note="fragment of the pyrFE genes from Staphylococcus
                     aureus; replacing the pC194 origin of replication"
     CDS             2144..2791
                     /gene="cat"
                     /label="Chloramphenicol acetyltransferase"
                     /note="Chloramphenicol acetyltransferase from
                     Staphylococcus aureus. Accession#: P00485"
     misc_feature    complement(2939..3046)
                     /label="MCS"
                     /note="pBluescript multiple cloning site"
     promoter        complement(3055..3073)
                     /label="T7 promoter"
                     /note="promoter for bacteriophage T7 RNA polymerase"
     rep_origin      3522..3744
                     /label="pSC101 ori"
                     /note="low-copy replication origin that requires the Rep101
                     protein"
     CDS             3792..4739
                     /label="Rep101"
                     /note="RepA protein needed for replication with the pSC101
                     origin"
     CDS             5994..6782
                     /label="SmR"
                     /note="aminoglycoside adenylyltransferase (Murphy, 1985)"
ORIGIN
        1 ggcgcgccag atcgcgtgca taattcaaca gctaacctcc caataactac gcgtcggctt
       61 aaaccagttt tcgttggtgc gaaaaaagag tgtcttgtga cactcttaaa ttcaaaatct
      121 atcggtcaga tttataccga tttgatttta tatattcttg aataacatac gccgagttat
      181 cacataaaag cgggaaccaa tcatcaaatt taaacttcat tgcataatcc attaaactct
      241 taaattctac gattccttgt tcatcaataa actcaatcat ttctttaatt aatttatatc
      301 tatctgttgt tgttttcttt aataattcat caacatctac accgccataa actatcatat
      361 cttctttttg atatttaaat ttattaggat cgtccatgtg aagcatatat ctcacaagac
      421 ctttcacact tcctgcaatc tgcggaatag tcgcattcaa ttcttctgta attattttta
      481 tctgttcata agatttatta ccctcataca tcactagaat atgataatgc tcttttttca
      541 tcctatcttc tgtatcagta tccctatcat gtaatggaga cactacaaat tgaatgtgta
      601 actcttttaa atactctaac cactcggctt ttgctgattc tggatataaa acaaatgtcc
      661 aattacgtcc tcttgaattt ttcttgtttt cagtttcttt tattacattt tcgctcatga
      721 tataataacg gtgctaatac atttaacaaa atttagtcat agataggcag catgccagtg
      781 ctgtctatct ttttttgttt aaaatgcacc gtattcctcc tttgcatatt tttttattag
      841 aataccggtt gcatctgatt tgctaatatt atatttttct ttgattctat ttaatatctc
      901 attttcttct gttgtaagtc ttaaagtaac agcaactttt ttctcttctt ttctatctac
      961 aaccatcact gtacctccca acatctgttt ttttcacttt aacataaaaa acaacctttt
     1021 aacattaaaa acccaatatt tatttatttg tttggacaat ggacaatgga cacctagggg
     1081 ggaggtcgta gtacccccct atgttttctc ccctaaataa ccccaaaaat ctaagaaaaa
     1141 aagacctcaa aaaggtcttt aattaacatc tcaaatttcg catttattcc aatttccttt
     1201 ttgcgtgtcc atggtacagt tggtaaggcg atggaaggac tagctaaatt gaatgttgat
     1261 ctggtaaatg ttcatgctgc tggtggcgta aaaatgatgt ctgaggccat taaaggatta
     1321 agaaaacata atcaacatac aaaaattatt gcagtaacac agcttacgtc aacaacagaa
     1381 gacatgttac gacacgaaca aaatatacaa acatcgattg aagaggccgt tttaaattat
     1441 gccaagttag caaatgcagc tggtttagat ggcgttgttt gttcacctct tgaaagtcgt
     1501 atgttgactg aaaagttagg tacatcattt ttaaaagtaa caccaggtat tagacctaaa
     1561 ggtgcatctc aagatgacca acaccgtatt acgacaccgg aagaagcaag acagcttggt
     1621 tcgacgcata ttgtagtcgg tagaccgatt acacaaagtg acaatccagt cgaaagttat
     1681 cataaaatta aagaaagttg gttagtataa tggctaaaga aattgcaaaa tcattattag
     1741 atattgaagc tgtaacatta tcaccaaatg atttatatac atggagttca ggtattaaat
     1801 caccgattta ctgtgataac cgtgttacgt taggttatcc tttagttcga ggcgcaatcc
     1861 gcgatgtaca tgtattctca agataagaaa gaaaaggatt tttcgctacg ctcaaatcct
     1921 ttaaaaaaac acaaaagacc acatttttta atgtggtctt ttattcttca actaaagcac
     1981 ccattagttc aacaaacgaa aattggataa agtgggatat ttttaaaata tatatttatg
     2041 ttacagtaat attgactttt aaaaaaggat tgattctaat gaagaaagca gacaagtaag
     2101 cctcctaaat tcactttaga taaaaattta ggaggcacat caaatgaact ttaataaaat
     2161 tgatttagac aattggaaga gaaaagagat atttaatcat tatttgaacc aacaaacgac
     2221 ttttagtata accacagaaa ttgatattag tgttttatac cgaaacataa aacaggaagg
     2281 atataaattt taccctgcat ttattttctt agtgacaagg gtgataaact caaatacagc
     2341 ttttagaact ggttacaata gcgacggaga gttaggttat tgggataagt tagagccact
     2401 ttatacaatt tttgatggtg tatctaaaac attctctggt atttggactc ctgtaaagaa
     2461 tgacttcaaa gagttttatg atttatacct ttctgatgta gagaaatata atggttcggg
     2521 gaaattgttt cccaaaacac ctatacctga aaatgctttt tctctttcta ttattccttg
     2581 gacttcattt actgggttta acttaaatat caataataat agtaattacc ttctacccat
     2641 tattacagca ggaaaattca ttaataaagg taattcaata tatttaccgc tatctttaca
     2701 ggtacatcat tctgtttgtg atggttatca tgcaggattg tttatgaact ctattcagga
     2761 attgtcagat aggcctaatg actggctttt ataatatgag ataatgccga ctgtactttt
     2821 tacagtcggt tttctaatgt cactaacctg ccccgttagt tgaagattcg aatgccccgt
     2881 tagttgaaga aggtttttat attacagctc cagatctcac taaagggaac aaaagctggg
     2941 taccgggccc cccctcgagg tcgacggtat cgataagctt gatatcgaat tcctgcagcc
     3001 cgggggatcc actagttcta gagcggccgc caccgcggtg gagctccaat tcgccctata
     3061 gtgagtcgta ttaggcgcgc cttactgggt gcattagcca gtctgaatga cctgtcacgg
     3121 gataatccga agtggtcaga ctggaaaatc agagggcagg aactgctgaa cagcaaaaag
     3181 tcagatagca ccacatagca gacccgccat aaaacgccct gagaagcccg tgacgggctt
     3241 ttcttgtatt atgggtagtt tccttgcatg aatccataaa aggcgcctgt agtgccattt
     3301 acccccattc actgccagag ccgtgagcgc agcgaactga atgtcacgaa aaagacagcg
     3361 actcaggtgc ctgatggtcg gagacaaaag gaatattcag cgatttgccc gagcttgcga
     3421 gggtgctact taagccttta gggttttaag gtctgttttg tagaggagca aacagcgttt
     3481 gcgacatcct tttgtaatac tgcggaactg actaaagtag tgagttatac acagggctgg
     3541 gatctattct ttttatcttt ttttattctt tctttattct ataaattata accacttgaa
     3601 tataaacaaa aaaaacacac aaaggtctag cggaatttac agagggtcta gcagaattta
     3661 caagttttcc agcaaaggtc tagcagaatt tacagatacc cacaactcaa aggaaaagga
     3721 ctagtaatta tcattgacta gcccatctca attggtatag tgattaaaat cacctagacc
     3781 aattgagatg tatgtctgaa ttagttgttt tcaaagcaaa tgaactagcg attagtcgct
     3841 atgacttaac ggagcatgaa accaagctaa ttttatgctg tgtggcacta ctcaacccca
     3901 cgattgaaaa ccctacaagg aaagaacgga cggtatcgtt cacttataac caatacgctc
     3961 agatgatgaa catcagtagg gaaaatgctt atggtgtatt agctaaagca accagagagc
     4021 tgatgacgag aactgtggaa atcaggaatc ctttggttaa aggctttaag attttccagt
     4081 ggacaaacta tgccaagttc tcaagcgaaa aattagaatt agtttttagt gaagagatat
     4141 tgccttatct tttccagtta aaaaaattca taaaatataa tctggaacat gttaagtctt
     4201 ttgaaaacaa atactctatg aggatttatg agtggttatt aaaagaacta acacaaaaga
     4261 aaactcacaa ggcaaatata gagattagcc ttgatgaatt taagttcatg ttaatgcttg
     4321 aaaataacta ccatgagttt aaaaggctta accaatgggt tttgaaacca ataagtaaag
     4381 atttaaacac ttacagcaat atgaaattgg tggttgataa gcgaggccgc ccgactgata
     4441 cgttgatttt ccaagttgaa ctagatagac aaatggatct cgtaaccgaa cttgagaaca
     4501 accagataaa aatgaatggt gacaaaatac caacaaccat tacatcagat tcctacctac
     4561 gtaacggact aagaaaaaca ctacacgatg ctttaactgc aaaaattcag ctcaccagtt
     4621 ttgaggcaaa atttttgagt gacatgcaaa gtaagtatga tctcaatggt tcgttctcat
     4681 ggctcacgca aaaacaacga accacactag agaacatact ggctaaatac ggaaggatct
     4741 gaggttctta tggctcttgt atctatcagt gaagcatcaa gactaacaaa caaaagtaga
     4801 acaactgttc accgttacat atcaaaggga aaactgtcca tatgcacaga tgaaaacggt
     4861 gtaaaaaaga tagatacatc agagctttta cgagtttttg gtgcattcaa agctgttcac
     4921 catgaacaga tcgacaatgt aacagatgaa cagcatgtaa cacctaatag aacaggtgaa
     4981 accagtaaaa caaagcaact agaacatgaa attgaacacc tgagacaact tgttacagct
     5041 caacagtcac acatagacag cctgaaacag gcgatgctgc ttatcgaatc aaagctgccg
     5101 acaacacggg agccagtgac gcctcccgtg gggaaaaaat catggcaatt ctggaagaaa
     5161 tagcgctttc agccggcaaa ccggctgaag ccggatctgc gattctgata acaaactagc
     5221 aacaccagaa cagcccgttt gcgggcagca aaacccgtgg gaattaattc ccctgctcgc
     5281 gcaggctggg tgccaagctc tcgggtaaca tcaaggcccg atccttggag cccttgccct
     5341 cccgcacgat gatcgtgccg tgatcgaaat ccagatcctt gacccgcagt tgcaaaccct
     5401 cactgatccg catgcccgtt ccatacagaa gctgggcgaa caaacgatgc tcgccttcca
     5461 gaaaaccgag gatgcgaacc acttcatccg gggtcagcac caccggcaag cgccgcgacg
     5521 gccgaggtct tccgatctcc tgaagccagg gcagatccgt gcacagcacc ttgccgtaga
     5581 agaacagcaa ggccgccaat gcctgacgat gcgtggagac cgaaaccttg cgctcgttcg
     5641 ccagccagga cagaaatgcc tcgacttcgc tgctgcccaa ggttgccggg tgacgcacac
     5701 cgtggaaacg gatgaaggca cgaacccagt ggacataagc ctgttcggtt cgtaagctgt
     5761 aatgcaagta gcgtatgcgc tcacgcaact ggtccagaac cttgaccgaa cgcagcggtg
     5821 gtaacggcgc agtggcggtt ttcatggctt gttatgactg tttttttggg gtacagtcta
     5881 tgcctcgggc atccaagcag caagcgcgtt acgccgtggg tcgatgtttg atgttatgga
     5941 gcagcaacga tgttacgcag cagggcagtc gccctaaaac aaagttaaac atcatgaggg
     6001 aagcggtgat cgccgaagta tcgactcaac tatcagaggt agttggcgtc atcgagcgcc
     6061 atctcgaacc gacgttgctg gccgtacatt tgtacggctc cgcagtggat ggcggcctga
     6121 agccacacag tgatattgat ttgctggtta cggtgaccgt aaggcttgat gaaacaacgc
     6181 ggcgagcttt gatcaacgac cttttggaaa cttcggcttc ccctggagag agcgagattc
     6241 tccgcgctgt agaagtcacc attgttgtgc acgacgacat cattccgtgg cgttatccag
     6301 ctaagcgcga actgcaattt ggagaatggc agcgcaatga cattcttgca ggtatcttcg
     6361 agccagccac gatcgacatt gatctggcta tcttgctgac aaaagcaaga gaacatagcg
     6421 ttgccttggt aggtccagcg gcggaggaac tctttgatcc ggttcctgaa caggatctat
     6481 ttgaggcgct aaatgaaacc ttaacgctat ggaactcgcc gcccgactgg gctggcgatg
     6541 agcgaaatgt agtgcttacg ttgtcccgca tttggtacag cgcagtaacc ggcaaaatcg
     6601 cgccgaagga tgtcgctgcc gactgggcaa tggagcgcct gccggcccag tatcagcccg
     6661 tcatacttga agctagacag gcttatcttg gacaagaaga agatcgcttg gcctcgcgcg
     6721 cagatcagtt ggaagaattt gtccactacg tgaaaggcga gatcaccaag gtagtcggca
     6781 aataatgtct aacaattcgt tcaagccgac gccgcttcgc ggcgcggctt aactcaagcg
     6841 ttagatgcac taagcacata attgctcaca gccaaactat caggtcaagt ctgcttttat
     6901 tatttttaag cgtgcataat aagccctaca caaattggga gatatatcat gaaaggctgg
     6961 ctttttcttg ttatcgcaat agttggcgaa gtaatcgcaa catccgca
//
