LOCUS       V003705                 7468 bp    DNA     circular SYN 18-DEC-2018
DEFINITION  Exported.
ACCESSION   V003705
VERSION     V003705
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
            .
REFERENCE   1  (bases 1 to 7468)
  AUTHORS   Redder P, Linder P.
  TITLE     New Range of Vectors with a Stringent 5-Fluoroorotic Acid-Based
            Counterselection System for Generating Mutants by Allelic
            Replacement in Staphylococcus aureus
  JOURNAL   Appl. Environ. Microbiol. 78 (11), 3846-3854 (2012)
   PUBMED   22447609
REFERENCE   2  (bases 1 to 7468)
  AUTHORS   Redder P, Linder P.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2012) Department of Microbiology and Molecular
            Medicine, University of Geneva, Faculty of Medicine, Rue
            Michel-Servet 1, Geneva 1203, Switzerland
REFERENCE   3  (bases 1 to 7468)
  TITLE     Direct Submission
REFERENCE   4  (bases 1 to 7468)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     ##Assembly-Data-START##
            Assembly Method       :: BioEdit, CAP v. 7.0.9.0
            Sequencing Technology :: Sanger dideoxy sequencing
            ##Assembly-Data-END##
            SGRef: number: 1; type: "Journal Article"; journalName: "Appl.
            Environ. Microbiol."; date: "2012"; volume: "78"; issue: "11";
            pages: "3846-3854"
            SGRef: number: 2; type: "Journal Article"; journalName: "Submitted
            (12-MAR-2012) Department of Microbiology and Molecular Medicine,
            University of Geneva, Faculty of Medicine, Rue Michel-Servet 1,
            Geneva 1203, Switzerland"
            SGRef: number: 3; type: "Journal Article"
FEATURES             Location/Qualifiers
     source          1..7468
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     rep_origin      complement(50..1669)
                     /direction=LEFT
                     /note="thermosensitive origin of replication; for
                     replication in Staphylococcus aureus.; pT181ts"
     misc_feature    1670..2325
                     /note="fragment of the pyrFE genes from Staphylococcus
                     aureus; replacing the pC194 origin of replication"
     CDS             2604..3251
                     /gene="cat"
                     /label="Chloramphenicol acetyltransferase"
                     /note="Chloramphenicol acetyltransferase from
                     Staphylococcus aureus. Accession#: P00485"
     misc_feature    complement(3399..3506)
                     /label="MCS"
                     /note="pBluescript multiple cloning site"
     promoter        complement(3515..3533)
                     /label="T7 promoter"
                     /note="promoter for bacteriophage T7 RNA polymerase"
     rep_origin      3982..4204
                     /label="pSC101 ori"
                     /note="low-copy replication origin that requires the Rep101
                     protein"
     CDS             4252..5199
                     /label="Rep101"
                     /note="RepA protein needed for replication with the pSC101
                     origin"
     CDS             6454..7242
                     /label="SmR"
                     /note="aminoglycoside adenylyltransferase (Murphy, 1985)"
ORIGIN
        1 ggcgcgccag atcgcgtgca taattcaaca gctaacctcc caataactac gcgtctcttt
       61 acttgaggtg actaaagttt ataggggtaa attattcttt cacctaatat aaattcccca
      121 aatttttaaa tagacacttc ataaaaaaat cctcctaaat tagaatagtt taaatatatc
      181 atttttgttc agtaatatta atatgtacgt acactatttc caaaatttaa attcatgttg
      241 ccaaaaatcg atttgttttt gcaattgttt ttcgttcgct tttaaagtcg atttcattaa
      301 gtccgttaaa tcgactggcg aaatttcttt tatcaaattc ttatattttg ttctggaatt
      361 tctgtgtagc tttccccatt cttcttcatc actcaataac ataaaaacta ttgctctatc
      421 cgcagtgcgt tggatagttt tccaatctgg ttgcaagata tgtaaatcac taaagcaatc
      481 attccagtaa tccaccatat ctcttttaaa ttcgatttct acacgccata aatgttcaga
      541 cataacttca gcatctgcat tatctttacg ttcttgcttt ttattataaa ttctaataaa
      601 tctattacta tctctcacgc caaaatattt tgtttctggc ttaccattac gaccataaaa
      661 aatagttttc ttaactgctt tatcagacat tgcatagtag tcactcaaat catcttcaaa
      721 atcaaaggct aaatctaatc ttgtaaaacc gtcatcttcc atgtagctta ttatattttg
      781 ttttaaccaa atcatttcat ctcgtgtaag tttgtttgga ttaaattcaa tacgcatatt
      841 acgtctatcc caactatctg ctttcacttt gtcatattca atataaactt tttcttgaag
      901 tgctttagct ttaaactttg tttgaagaat atcccaaagt cttatttggg gctctacact
      961 cataaattta gaaagggctt gagcgttgtc tcggttgaga tttccaacga tcgtcattgc
     1021 atcaaaactt aattttggat cagatatgca cacagtatgt gcgtccaacc ggctattaga
     1081 gtagccggtt ttagaaaaat tgtctaaatc gtgattttcc aaatgatttg aatgatttgc
     1141 atgattgttt ttatacataa aaaatcgact ccttaatctc aatttcgttt aaggaatcgc
     1201 tcacccaaat atatatcttg atgtatattt aaatatcgtt taatatctaa atatacaaga
     1261 ttataaaaac aactcagtgt ttttttcttt gaatgatgtc gttcacaaac tttggtcagg
     1321 tcgtgagcga ctccttttta ttttgttatt aatataacac tatcaaaaga tttggtctaa
     1381 tcagatcaag tcttttttta tttaagcatt tgtattatct ggtaaacagt taaaactact
     1441 aaaacaccaa gtacatactt acttgttata aaattctcaa aggcttctct gaatttcatt
     1501 tcttcaactc cttttattgc tttattccaa tttccttatt ggtcggaacc tacagggctt
     1561 tatgtgttgt tgagttggta ctttcttggg attaatccca attcaagtcc aaccaactcg
     1621 ctaacaagtt agctaacaca tagcccattc caaccaataa gttttctccc atggtacagt
     1681 tggtaaggcg atggaaggac tagctaaatt gaatgttgat ctggtaaatg ttcatgctgc
     1741 tggtggcgta aaaatgatgt ctgaggccat taaaggatta agaaaacata atcaacatac
     1801 aaaaattatt gcagtaacac agcttacgtc aacaacagaa gacatgttac gacacgaaca
     1861 aaatatacaa acatcgattg aagaggccgt tttaaattat gccaagttag caaatgcagc
     1921 tggtttagat ggcgttgttt gttcacctct tgaaagtcgt atgttgactg aaaagttagg
     1981 tacatcattt ttaaaagtaa caccaggtat tagacctaaa ggtgcatctc aagatgacca
     2041 acaccgtatt acgacaccgg aagaagcaag acagcttggt tcgacgcata ttgtagtcgg
     2101 tagaccgatt acacaaagtg acaatccagt cgaaagttat cataaaatta aagaaagttg
     2161 gttagtataa tggctaaaga aattgcaaaa tcattattag atattgaagc tgtaacatta
     2221 tcaccaaatg atttatatac atggagttca ggtattaaat caccgattta ctgtgataac
     2281 cgtgttacgt taggttatcc tttagttcga ggcgcaatcc gcgatgtaca tgtattctca
     2341 agataagaaa gaaaaggatt tttcgctacg ctcaaatcct ttaaaaaaac acaaaagacc
     2401 acatttttta atgtggtctt ttattcttca actaaagcac ccattagttc aacaaacgaa
     2461 aattggataa agtgggatat ttttaaaata tatatttatg ttacagtaat attgactttt
     2521 aaaaaaggat tgattctaat gaagaaagca gacaagtaag cctcctaaat tcactttaga
     2581 taaaaattta ggaggcacat caaatgaact ttaataaaat tgatttagac aattggaaga
     2641 gaaaagagat atttaatcat tatttgaacc aacaaacgac ttttagtata accacagaaa
     2701 ttgatattag tgttttatac cgaaacataa aacaggaagg atataaattt taccctgcat
     2761 ttattttctt agtgacaagg gtgataaact caaatacagc ttttagaact ggttacaata
     2821 gcgacggaga gttaggttat tgggataagt tagagccact ttatacaatt tttgatggtg
     2881 tatctaaaac attctctggt atttggactc ctgtaaagaa tgacttcaaa gagttttatg
     2941 atttatacct ttctgatgta gagaaatata atggttcggg gaaattgttt cccaaaacac
     3001 ctatacctga aaatgctttt tctctttcta ttattccttg gacttcattt actgggttta
     3061 acttaaatat caataataat agtaattacc ttctacccat tattacagca ggaaaattca
     3121 ttaataaagg taattcaata tatttaccgc tatctttaca ggtacatcat tctgtttgtg
     3181 atggttatca tgcaggattg tttatgaact ctattcagga attgtcagat aggcctaatg
     3241 actggctttt ataatatgag ataatgccga ctgtactttt tacagtcggt tttctaatgt
     3301 cactaacctg ccccgttagt tgaagattcg aatgccccgt tagttgaaga aggtttttat
     3361 attacagctc cagatctcac taaagggaac aaaagctggg taccgggccc cccctcgagg
     3421 tcgacggtat cgataagctt gatatcgaat tcctgcagcc cgggggatcc actagttcta
     3481 gagcggccgc caccgcggtg gagctccaat tcgccctata gtgagtcgta ttaggcgcgc
     3541 cttactgggt gcattagcca gtctgaatga cctgtcacgg gataatccga agtggtcaga
     3601 ctggaaaatc agagggcagg aactgctgaa cagcaaaaag tcagatagca ccacatagca
     3661 gacccgccat aaaacgccct gagaagcccg tgacgggctt ttcttgtatt atgggtagtt
     3721 tccttgcatg aatccataaa aggcgcctgt agtgccattt acccccattc actgccagag
     3781 ccgtgagcgc agcgaactga atgtcacgaa aaagacagcg actcaggtgc ctgatggtcg
     3841 gagacaaaag gaatattcag cgatttgccc gagcttgcga gggtgctact taagccttta
     3901 gggttttaag gtctgttttg tagaggagca aacagcgttt gcgacatcct tttgtaatac
     3961 tgcggaactg actaaagtag tgagttatac acagggctgg gatctattct ttttatcttt
     4021 ttttattctt tctttattct ataaattata accacttgaa tataaacaaa aaaaacacac
     4081 aaaggtctag cggaatttac agagggtcta gcagaattta caagttttcc agcaaaggtc
     4141 tagcagaatt tacagatacc cacaactcaa aggaaaagga ctagtaatta tcattgacta
     4201 gcccatctca attggtatag tgattaaaat cacctagacc aattgagatg tatgtctgaa
     4261 ttagttgttt tcaaagcaaa tgaactagcg attagtcgct atgacttaac ggagcatgaa
     4321 accaagctaa ttttatgctg tgtggcacta ctcaacccca cgattgaaaa ccctacaagg
     4381 aaagaacgga cggtatcgtt cacttataac caatacgctc agatgatgaa catcagtagg
     4441 gaaaatgctt atggtgtatt agctaaagca accagagagc tgatgacgag aactgtggaa
     4501 atcaggaatc ctttggttaa aggctttaag attttccagt ggacaaacta tgccaagttc
     4561 tcaagcgaaa aattagaatt agtttttagt gaagagatat tgccttatct tttccagtta
     4621 aaaaaattca taaaatataa tctggaacat gttaagtctt ttgaaaacaa atactctatg
     4681 aggatttatg agtggttatt aaaagaacta acacaaaaga aaactcacaa ggcaaatata
     4741 gagattagcc ttgatgaatt taagttcatg ttaatgcttg aaaataacta ccatgagttt
     4801 aaaaggctta accaatgggt tttgaaacca ataagtaaag atttaaacac ttacagcaat
     4861 atgaaattgg tggttgataa gcgaggccgc ccgactgata cgttgatttt ccaagttgaa
     4921 ctagatagac aaatggatct cgtaaccgaa cttgagaaca accagataaa aatgaatggt
     4981 gacaaaatac caacaaccat tacatcagat tcctacctac gtaacggact aagaaaaaca
     5041 ctacacgatg ctttaactgc aaaaattcag ctcaccagtt ttgaggcaaa atttttgagt
     5101 gacatgcaaa gtaagtatga tctcaatggt tcgttctcat ggctcacgca aaaacaacga
     5161 accacactag agaacatact ggctaaatac ggaaggatct gaggttctta tggctcttgt
     5221 atctatcagt gaagcatcaa gactaacaaa caaaagtaga acaactgttc accgttacat
     5281 atcaaaggga aaactgtcca tatgcacaga tgaaaacggt gtaaaaaaga tagatacatc
     5341 agagctttta cgagtttttg gtgcattcaa agctgttcac catgaacaga tcgacaatgt
     5401 aacagatgaa cagcatgtaa cacctaatag aacaggtgaa accagtaaaa caaagcaact
     5461 agaacatgaa attgaacacc tgagacaact tgttacagct caacagtcac acatagacag
     5521 cctgaaacag gcgatgctgc ttatcgaatc aaagctgccg acaacacggg agccagtgac
     5581 gcctcccgtg gggaaaaaat catggcaatt ctggaagaaa tagcgctttc agccggcaaa
     5641 ccggctgaag ccggatctgc gattctgata acaaactagc aacaccagaa cagcccgttt
     5701 gcgggcagca aaacccgtgg gaattaattc ccctgctcgc gcaggctggg tgccaagctc
     5761 tcgggtaaca tcaaggcccg atccttggag cccttgccct cccgcacgat gatcgtgccg
     5821 tgatcgaaat ccagatcctt gacccgcagt tgcaaaccct cactgatccg catgcccgtt
     5881 ccatacagaa gctgggcgaa caaacgatgc tcgccttcca gaaaaccgag gatgcgaacc
     5941 acttcatccg gggtcagcac caccggcaag cgccgcgacg gccgaggtct tccgatctcc
     6001 tgaagccagg gcagatccgt gcacagcacc ttgccgtaga agaacagcaa ggccgccaat
     6061 gcctgacgat gcgtggagac cgaaaccttg cgctcgttcg ccagccagga cagaaatgcc
     6121 tcgacttcgc tgctgcccaa ggttgccggg tgacgcacac cgtggaaacg gatgaaggca
     6181 cgaacccagt ggacataagc ctgttcggtt cgtaagctgt aatgcaagta gcgtatgcgc
     6241 tcacgcaact ggtccagaac cttgaccgaa cgcagcggtg gtaacggcgc agtggcggtt
     6301 ttcatggctt gttatgactg tttttttggg gtacagtcta tgcctcgggc atccaagcag
     6361 caagcgcgtt acgccgtggg tcgatgtttg atgttatgga gcagcaacga tgttacgcag
     6421 cagggcagtc gccctaaaac aaagttaaac atcatgaggg aagcggtgat cgccgaagta
     6481 tcgactcaac tatcagaggt agttggcgtc atcgagcgcc atctcgaacc gacgttgctg
     6541 gccgtacatt tgtacggctc cgcagtggat ggcggcctga agccacacag tgatattgat
     6601 ttgctggtta cggtgaccgt aaggcttgat gaaacaacgc ggcgagcttt gatcaacgac
     6661 cttttggaaa cttcggcttc ccctggagag agcgagattc tccgcgctgt agaagtcacc
     6721 attgttgtgc acgacgacat cattccgtgg cgttatccag ctaagcgcga actgcaattt
     6781 ggagaatggc agcgcaatga cattcttgca ggtatcttcg agccagccac gatcgacatt
     6841 gatctggcta tcttgctgac aaaagcaaga gaacatagcg ttgccttggt aggtccagcg
     6901 gcggaggaac tctttgatcc ggttcctgaa caggatctat ttgaggcgct aaatgaaacc
     6961 ttaacgctat ggaactcgcc gcccgactgg gctggcgatg agcgaaatgt agtgcttacg
     7021 ttgtcccgca tttggtacag cgcagtaacc ggcaaaatcg cgccgaagga tgtcgctgcc
     7081 gactgggcaa tggagcgcct gccggcccag tatcagcccg tcatacttga agctagacag
     7141 gcttatcttg gacaagaaga agatcgcttg gcctcgcgcg cagatcagtt ggaagaattt
     7201 gtccactacg tgaaaggcga gatcaccaag gtagtcggca aataatgtct aacaattcgt
     7261 tcaagccgac gccgcttcgc ggcgcggctt aactcaagcg ttagatgcac taagcacata
     7321 attgctcaca gccaaactat caggtcaagt ctgcttttat tatttttaag cgtgcataat
     7381 aagccctaca caaattggga gatatatcat gaaaggctgg ctttttcttg ttatcgcaat
     7441 agttggcgaa gtaatcgcaa catccgca
//
