LOCUS       V003706                 6575 bp    DNA     circular SYN 18-DEC-2018
DEFINITION  Exported.
ACCESSION   V003706
VERSION     V003706
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
            .
REFERENCE   1  (bases 1 to 6575)
  AUTHORS   Redder P, Linder P.
  TITLE     New Range of Vectors with a Stringent 5-Fluoroorotic Acid-Based
            Counterselection System for Generating Mutants by Allelic
            Replacement in Staphylococcus aureus
  JOURNAL   Appl. Environ. Microbiol. 78 (11), 3846-3854 (2012)
   PUBMED   22447609
REFERENCE   2  (bases 1 to 6575)
  AUTHORS   Redder P, Linder P.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2012) Department of Microbiology and Molecular
            Medicine, University of Geneva, Faculty of Medicine, Rue
            Michel-Servet 1, Geneva 1203, Switzerland
REFERENCE   3  (bases 1 to 6575)
  TITLE     Direct Submission
REFERENCE   4  (bases 1 to 6575)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     ##Assembly-Data-START##
            Assembly Method       :: BioEdit, CAP v. 7.0.9.0
            Sequencing Technology :: Sanger dideoxy sequencing
            ##Assembly-Data-END##
            SGRef: number: 1; type: "Journal Article"; journalName: "Appl.
            Environ. Microbiol."; date: "2012"; volume: "78"; issue: "11";
            pages: "3846-3854"
            SGRef: number: 2; type: "Journal Article"; journalName: "Submitted
            (12-MAR-2012) Department of Microbiology and Molecular Medicine,
            University of Geneva, Faculty of Medicine, Rue Michel-Servet 1,
            Geneva 1203, Switzerland"
            SGRef: number: 3; type: "Journal Article"
FEATURES             Location/Qualifiers
     source          1..6575
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     CDS             1485..2132
                     /gene="cat"
                     /label="Chloramphenicol acetyltransferase"
                     /note="Chloramphenicol acetyltransferase from
                     Staphylococcus aureus. Accession#: P00485"
     misc_feature    2261..2505
                     /note="derived from pLP763; Pprot promoter"
     misc_feature    complement(2506..2613)
                     /label="MCS"
                     /note="pBluescript multiple cloning site"
     promoter        complement(2622..2640)
                     /label="T7 promoter"
                     /note="promoter for bacteriophage T7 RNA polymerase"
     rep_origin      3089..3311
                     /label="pSC101 ori"
                     /note="low-copy replication origin that requires the Rep101
                     protein"
     CDS             3359..4306
                     /label="Rep101"
                     /note="RepA protein needed for replication with the pSC101
                     origin"
     CDS             5561..6349
                     /label="SmR"
                     /note="aminoglycoside adenylyltransferase (Murphy, 1985)"
ORIGIN
        1 ggcgcgccag atcgcgtgca taattcaaca gctaacctcc caataactac atggtgttat
       61 cgggaggtca gctgttagca cttatatttt gttattgttc ttcctcgatt tcgtctatca
      121 ttttgtgatt aatttctctt ttttcttgtt ctgttaagtc ataaagttca ctagctaaat
      181 actctttttg tttccaaata taaaaaattt gatagatata ttcggttgga tcaatttctt
      241 ttaagtaatc taaatcccca ttttttaatt tctttttagc ctctttaaat aatcctgaat
      301 aaactaatac ctgtttacct ttaagtgatt tataaaatgc atcaaagact ttttgattta
      361 ttaaataatc actatcttta ccagaatact tagccatttc atataattct ttattattat
      421 tttgtcttat tttttgaact tgaacttgtg ttatttctga aatgcccgtt acatcacgcc
      481 ataaatctaa ccattcttgt tggctaatat aatatctttt atctgtgaaa tacgatttat
      541 ttactgcaat taacacatga aaatgaggat tataatcatc tcttttttta ttatatgtaa
      601 tctctaactt acgaacatat ccctttataa cactacctac tttttttctc tttataagtt
      661 ttctaaaaga attattataa cgttttattt cattttctaa ttcatcactc attacattag
      721 gtgtagtcaa agttaaaaag ataaactcct ttttctcttg ctgcttaata tattgcatca
      781 tcaaagataa acccaatgca tcttttctag cttttctcca agcacagaca ggacaaaatc
      841 gatttttaca agaattagct ttatataatt tctgtttttc taaagtttta tcagctacaa
      901 aagacagaaa tgtattgcaa tcttcaacta aatccatttg attctctcca atatgacgtt
      961 taataaattt ctgaaatact tgatttcttt gttttttctc agtatacttt tccatgttat
     1021 aacacataaa aacaacttag ttttcacaaa ctatgacaat aaaaaaagtt gctttttccc
     1081 ctttctatgt atgtttttta ctagtcattt aaaacgatac attaataggt acgaaaaagc
     1141 aacttttttt gcgcttaaaa ccagtcatac caataactta agggtaacta gcctcgccgg
     1201 caatagttac ccttattatc aagataagaa agaaaaggat ttttcgctac gctcaaatcc
     1261 tttaaaaaaa cacaaaagac cacatttttt aatgtggtct tttattcttc aactaaagca
     1321 cccattagtt caacaaacga aaattggata aagtgggata tttttaaaat atatatttat
     1381 gttacagtaa tattgacttt taaaaaagga ttgattctaa tgaagaaagc agacaagtaa
     1441 gcctcctaaa ttcactttag ataaaaattt aggaggcata tcaaatgaac tttaataaaa
     1501 ttgatttaga caattggaag agaaaagaga tatttaatca ttatttgaac caacaaacga
     1561 cttttagtat aaccacagaa attgatatta gtgttttata ccgaaacata aaacaagaag
     1621 gatataaatt ttaccctgca tttattttct tagtgacaag ggtgataaac tcaaatacag
     1681 cttttagaac tggttacaat agcgacggag agttaggtta ttgggataag ttagagccac
     1741 tttatacaat ttttgatggt gtatctaaaa cattctctgg tatttggact cctgtaaaga
     1801 atgacttcaa agagttttat gatttatacc tttctgatgt agagaaatat aatggttcgg
     1861 ggaaattgtt tcccaaaaca cctatacctg aaaatgcttt ttctctttct attattccat
     1921 ggacttcatt tactgggttt aacttaaata tcaataataa tagtaattac cttctaccca
     1981 ttattacagc aggaaaattc attaataaag gtaattcaat atatttaccg ctatctttac
     2041 aggtacatca ttctgtttgt gatggttatc atgcaggatt gtttatgaac tctattcagg
     2101 aattgtcaga taggcctaat gactggcttt tataatatga gataatgccg actgtacttt
     2161 ttacagtcgg ttttctaatg tcactaacct gccccgttag ttgaagaagg tttttatatt
     2221 acagctccag atctcactaa agggaacaaa agctgggtac aaatactcat tttctaataa
     2281 tgctaaaaat ttcaaaacat ctatagtctg taaacggcta aataataacg ctaaaagtta
     2341 atttacagat aaaaaaatta atagaagatt aaaattttcg ttgaatttgt tcttcaatag
     2401 tatataatat aatagtatat aatattatat aatataatct taactacatc aagcgtaggg
     2461 tttgatttgg ttatgaaact tttggaaagt ggaggatatt ggatgggtac cgggcccccc
     2521 ctcgaggtcg acggtatcga taagcttgat atcgaattcc tgcagcccgg gggatccact
     2581 agttctagag cggccgccac cgcggtggag ctccaattcg ccctatagtg agtcgtatta
     2641 ggcgcgcctt actgggtgca ttagccagtc tgaatgacct gtcacgggat aatccgaagt
     2701 ggtcagactg gaaaatcaga gggcaggaac tgctgaacag caaaaagtca gatagcacca
     2761 catagcagac ccgccataaa acgccctgag aagcccgtga cgggcttttc ttgtattatg
     2821 ggtagtttcc ttgcatgaat ccataaaagg cgcctgtagt gccatttacc cccattcact
     2881 gccagagccg tgagcgcagc gaactgaatg tcacgaaaaa gacagcgact caggtgcctg
     2941 atggtcggag acaaaaggaa tattcagcga tttgcccgag cttgcgaggg tgctacttaa
     3001 gcctttaggg ttttaaggtc tgttttgtag aggagcaaac agcgtttgcg acatcctttt
     3061 gtaatactgc ggaactgact aaagtagtga gttatacaca gggctgggat ctattctttt
     3121 tatctttttt tattctttct ttattctata aattataacc acttgaatat aaacaaaaaa
     3181 aacacacaaa ggtctagcgg aatttacaga gggtctagca gaatttacaa gttttccagc
     3241 aaaggtctag cagaatttac agatacccac aactcaaagg aaaaggacta gtaattatca
     3301 ttgactagcc catctcaatt ggtatagtga ttaaaatcac ctagaccaat tgagatgtat
     3361 gtctgaatta gttgttttca aagcaaatga actagcgatt agtcgctatg acttaacgga
     3421 gcatgaaacc aagctaattt tatgctgtgt ggcactactc aaccccacga ttgaaaaccc
     3481 tacaaggaaa gaacggacgg tatcgttcac ttataaccaa tacgctcaga tgatgaacat
     3541 cagtagggaa aatgcttatg gtgtattagc taaagcaacc agagagctga tgacgagaac
     3601 tgtggaaatc aggaatcctt tggttaaagg ctttaagatt ttccagtgga caaactatgc
     3661 caagttctca agcgaaaaat tagaattagt ttttagtgaa gagatattgc cttatctttt
     3721 ccagttaaaa aaattcataa aatataatct ggaacatgtt aagtcttttg aaaacaaata
     3781 ctctatgagg atttatgagt ggttattaaa agaactaaca caaaagaaaa ctcacaaggc
     3841 aaatatagag attagccttg atgaatttaa gttcatgtta atgcttgaaa ataactacca
     3901 tgagtttaaa aggcttaacc aatgggtttt gaaaccaata agtaaagatt taaacactta
     3961 cagcaatatg aaattggtgg ttgataagcg aggccgcccg actgatacgt tgattttcca
     4021 agttgaacta gatagacaaa tggatctcgt aaccgaactt gagaacaacc agataaaaat
     4081 gaatggtgac aaaataccaa caaccattac atcagattcc tacctacgta acggactaag
     4141 aaaaacacta cacgatgctt taactgcaaa aattcagctc accagttttg aggcaaaatt
     4201 tttgagtgac atgcaaagta agtatgatct caatggttcg ttctcatggc tcacgcaaaa
     4261 acaacgaacc acactagaga acatactggc taaatacgga aggatctgag gttcttatgg
     4321 ctcttgtatc tatcagtgaa gcatcaagac taacaaacaa aagtagaaca actgttcacc
     4381 gttacatatc aaagggaaaa ctgtccatat gcacagatga aaacggtgta aaaaagatag
     4441 atacatcaga gcttttacga gtttttggtg cattcaaagc tgttcaccat gaacagatcg
     4501 acaatgtaac agatgaacag catgtaacac ctaatagaac aggtgaaacc agtaaaacaa
     4561 agcaactaga acatgaaatt gaacacctga gacaacttgt tacagctcaa cagtcacaca
     4621 tagacagcct gaaacaggcg atgctgctta tcgaatcaaa gctgccgaca acacgggagc
     4681 cagtgacgcc tcccgtgggg aaaaaatcat ggcaattctg gaagaaatag cgctttcagc
     4741 cggcaaaccg gctgaagccg gatctgcgat tctgataaca aactagcaac accagaacag
     4801 cccgtttgcg ggcagcaaaa cccgtgggaa ttaattcccc tgctcgcgca ggctgggtgc
     4861 caagctctcg ggtaacatca aggcccgatc cttggagccc ttgccctccc gcacgatgat
     4921 cgtgccgtga tcgaaatcca gatccttgac ccgcagttgc aaaccctcac tgatccgcat
     4981 gcccgttcca tacagaagct gggcgaacaa acgatgctcg ccttccagaa aaccgaggat
     5041 gcgaaccact tcatccgggg tcagcaccac cggcaagcgc cgcgacggcc gaggtcttcc
     5101 gatctcctga agccagggca gatccgtgca cagcaccttg ccgtagaaga acagcaaggc
     5161 cgccaatgcc tgacgatgcg tggagaccga aaccttgcgc tcgttcgcca gccaggacag
     5221 aaatgcctcg acttcgctgc tgcccaaggt tgccgggtga cgcacaccgt ggaaacggat
     5281 gaaggcacga acccagtgga cataagcctg ttcggttcgt aagctgtaat gcaagtagcg
     5341 tatgcgctca cgcaactggt ccagaacctt gaccgaacgc agcggtggta acggcgcagt
     5401 ggcggttttc atggcttgtt atgactgttt ttttggggta cagtctatgc ctcgggcatc
     5461 caagcagcaa gcgcgttacg ccgtgggtcg atgtttgatg ttatggagca gcaacgatgt
     5521 tacgcagcag ggcagtcgcc ctaaaacaaa gttaaacatc atgagggaag cggtgatcgc
     5581 cgaagtatcg actcaactat cagaggtagt tggcgtcatc gagcgccatc tcgaaccgac
     5641 gttgctggcc gtacatttgt acggctccgc agtggatggc ggcctgaagc cacacagtga
     5701 tattgatttg ctggttacgg tgaccgtaag gcttgatgaa acaacgcggc gagctttgat
     5761 caacgacctt ttggaaactt cggcttcccc tggagagagc gagattctcc gcgctgtaga
     5821 agtcaccatt gttgtgcacg acgacatcat tccgtggcgt tatccagcta agcgcgaact
     5881 gcaatttgga gaatggcagc gcaatgacat tcttgcaggt atcttcgagc cagccacgat
     5941 cgacattgat ctggctatct tgctgacaaa agcaagagaa catagcgttg ccttggtagg
     6001 tccagcggcg gaggaactct ttgatccggt tcctgaacag gatctatttg aggcgctaaa
     6061 tgaaacctta acgctatgga actcgccgcc cgactgggct ggcgatgagc gaaatgtagt
     6121 gcttacgttg tcccgcattt ggtacagcgc agtaaccggc aaaatcgcgc cgaaggatgt
     6181 cgctgccgac tgggcaatgg agcgcctgcc ggcccagtat cagcccgtca tacttgaagc
     6241 tagacaggct tatcttggac aagaagaaga tcgcttggcc tcgcgcgcag atcagttgga
     6301 agaatttgtc cactacgtga aaggcgagat caccaaggta gtcggcaaat aatgtctaac
     6361 aattcgttca agccgacgcc gcttcgcggc gcggcttaac tcaagcgtta gatgcactaa
     6421 gcacataatt gctcacagcc aaactatcag gtcaagtctg cttttattat ttttaagcgt
     6481 gcataataag ccctacacaa attgggagat atatcatgaa aggctggctt tttcttgtta
     6541 tcgcaatagt tggcgaagta atcgcaacat ccgca
//
