LOCUS       V003707                 6325 bp    DNA     circular SYN 18-DEC-2018
DEFINITION  Exported.
ACCESSION   V003707
VERSION     V003707
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
            .
REFERENCE   1  (bases 1 to 6325)
  AUTHORS   Redder P, Linder P.
  TITLE     New Range of Vectors with a Stringent 5-Fluoroorotic Acid-Based
            Counterselection System for Generating Mutants by Allelic
            Replacement in Staphylococcus aureus
  JOURNAL   Appl. Environ. Microbiol. 78 (11), 3846-3854 (2012)
   PUBMED   22447609
REFERENCE   2  (bases 1 to 6325)
  AUTHORS   Redder P, Linder P.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2012) Department of Microbiology and Molecular
            Medicine, University of Geneva, Faculty of Medicine, Rue
            Michel-Servet 1, Geneva 1203, Switzerland
REFERENCE   3  (bases 1 to 6325)
  TITLE     Direct Submission
REFERENCE   4  (bases 1 to 6325)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     ##Assembly-Data-START##
            Assembly Method       :: BioEdit, CAP v. 7.0.9.0
            Sequencing Technology :: Sanger dideoxy sequencing
            ##Assembly-Data-END##
            SGRef: number: 1; type: "Journal Article"; journalName: "Appl.
            Environ. Microbiol."; date: "2012"; volume: "78"; issue: "11";
            pages: "3846-3854"
            SGRef: number: 2; type: "Journal Article"; journalName: "Submitted
            (12-MAR-2012) Department of Microbiology and Molecular Medicine,
            University of Geneva, Faculty of Medicine, Rue Michel-Servet 1,
            Geneva 1203, Switzerland"
            SGRef: number: 3; type: "Journal Article"
FEATURES             Location/Qualifiers
     source          1..6325
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     CDS             1485..2132
                     /gene="cat"
                     /label="Chloramphenicol acetyltransferase"
                     /note="Chloramphenicol acetyltransferase from
                     Staphylococcus aureus. Accession#: P00485"
     misc_feature    complement(2256..2363)
                     /label="MCS"
                     /note="pBluescript multiple cloning site"
     promoter        complement(2372..2390)
                     /label="T7 promoter"
                     /note="promoter for bacteriophage T7 RNA polymerase"
     rep_origin      2839..3061
                     /label="pSC101 ori"
                     /note="low-copy replication origin that requires the Rep101
                     protein"
     CDS             3109..4056
                     /label="Rep101"
                     /note="RepA protein needed for replication with the pSC101
                     origin"
     CDS             5311..6099
                     /label="SmR"
                     /note="aminoglycoside adenylyltransferase (Murphy, 1985)"
ORIGIN
        1 ggcgcgccag atcgcgtgca taattcaaca gctaacctcc caataactac atggtgttat
       61 cgggaggtca gctgttagca cttatatttt gttattgttc ttcctcgatt tcgtctatca
      121 ttttgtgatt aatttctctt ttttcttgtt ctgttaagtc ataaagttca ctagctaaat
      181 actctttttg tttccaaata taaaaaattt gatagatata ttcggttgga tcaatttctt
      241 ttaagtaatc taaatcccca ttttttaatt tctttttagc ctctttaaat aatcctgaat
      301 aaactaatac ctgtttacct ttaagtgatt tataaaatgc atcaaagact ttttgattta
      361 ttaaataatc actatcttta ccagaatact tagccatttc atataattct ttattattat
      421 tttgtcttat tttttgaact tgaacttgtg ttatttctga aatgcccgtt acatcacgcc
      481 ataaatctaa ccattcttgt tggctaatat aatatctttt atctgtgaaa tacgatttat
      541 ttactgcaat taacacatga aaatgaggat tataatcatc tcttttttta ttatatgtaa
      601 tctctaactt acgaacatat ccctttataa cactacctac tttttttctc tttataagtt
      661 ttctaaaaga attattataa cgttttattt cattttctaa ttcatcactc attacattag
      721 gtgtagtcaa agttaaaaag ataaactcct ttttctcttg ctgcttaata tattgcatca
      781 tcaaagataa acccaatgca tcttttctag cttttctcca agcacagaca ggacaaaatc
      841 gatttttaca agaattagct ttatataatt tctgtttttc taaagtttta tcagctacaa
      901 aagacagaaa tgtattgcaa tcttcaacta aatccatttg attctctcca atatgacgtt
      961 taataaattt ctgaaatact tgatttcttt gttttttctc agtatacttt tccatgttat
     1021 aacacataaa aacaacttag ttttcacaaa ctatgacaat aaaaaaagtt gctttttccc
     1081 ctttctatgt atgtttttta ctagtcattt aaaacgatac attaataggt acgaaaaagc
     1141 aacttttttt gcgcttaaaa ccagtcatac caataactta agggtaacta gcctcgccgg
     1201 caatagttac ccttattatc aagataagaa agaaaaggat ttttcgctac gctcaaatcc
     1261 tttaaaaaaa cacaaaagac cacatttttt aatgtggtct tttattcttc aactaaagca
     1321 cccattagtt caacaaacga aaattggata aagtgggata tttttaaaat atatatttat
     1381 gttacagtaa tattgacttt taaaaaagga ttgattctaa tgaagaaagc agacaagtaa
     1441 gcctcctaaa ttcactttag ataaaaattt aggaggcata tcaaatgaac tttaataaaa
     1501 ttgatttaga caattggaag agaaaagaga tatttaatca ttatttgaac caacaaacga
     1561 cttttagtat aaccacagaa attgatatta gtgttttata ccgaaacata aaacaagaag
     1621 gatataaatt ttaccctgca tttattttct tagtgacaag ggtgataaac tcaaatacag
     1681 cttttagaac tggttacaat agcgacggag agttaggtta ttgggataag ttagagccac
     1741 tttatacaat ttttgatggt gtatctaaaa cattctctgg tatttggact cctgtaaaga
     1801 atgacttcaa agagttttat gatttatacc tttctgatgt agagaaatat aatggttcgg
     1861 ggaaattgtt tcccaaaaca cctatacctg aaaatgcttt ttctctttct attattccat
     1921 ggacttcatt tactgggttt aacttaaata tcaataataa tagtaattac cttctaccca
     1981 ttattacagc aggaaaattc attaataaag gtaattcaat atatttaccg ctatctttac
     2041 aggtacatca ttctgtttgt gatggttatc atgcaggatt gtttatgaac tctattcagg
     2101 aattgtcaga taggcctaat gactggcttt tataatatga gataatgccg actgtacttt
     2161 ttacagtcgg ttttctaatg tcactaacct gccccgttag ttgaagaagg tttttatatt
     2221 acagctccag atctcactaa agggaacaaa agctgggtac cgggcccccc ctcgaggtcg
     2281 acggtatcga taagcttgat atcgaattcc tgcagcccgg gggatccact agttctagag
     2341 cggccgccac cgcggtggag ctccaattcg ccctatagtg agtcgtatta ggcgcgcctt
     2401 actgggtgca ttagccagtc tgaatgacct gtcacgggat aatccgaagt ggtcagactg
     2461 gaaaatcaga gggcaggaac tgctgaacag caaaaagtca gatagcacca catagcagac
     2521 ccgccataaa acgccctgag aagcccgtga cgggcttttc ttgtattatg ggtagtttcc
     2581 ttgcatgaat ccataaaagg cgcctgtagt gccatttacc cccattcact gccagagccg
     2641 tgagcgcagc gaactgaatg tcacgaaaaa gacagcgact caggtgcctg atggtcggag
     2701 acaaaaggaa tattcagcga tttgcccgag cttgcgaggg tgctacttaa gcctttaggg
     2761 ttttaaggtc tgttttgtag aggagcaaac agcgtttgcg acatcctttt gtaatactgc
     2821 ggaactgact aaagtagtga gttatacaca gggctgggat ctattctttt tatctttttt
     2881 tattctttct ttattctata aattataacc acttgaatat aaacaaaaaa aacacacaaa
     2941 ggtctagcgg aatttacaga gggtctagca gaatttacaa gttttccagc aaaggtctag
     3001 cagaatttac agatacccac aactcaaagg aaaaggacta gtaattatca ttgactagcc
     3061 catctcaatt ggtatagtga ttaaaatcac ctagaccaat tgagatgtat gtctgaatta
     3121 gttgttttca aagcaaatga actagcgatt agtcgctatg acttaacgga gcatgaaacc
     3181 aagctaattt tatgctgtgt ggcactactc aaccccacga ttgaaaaccc tacaaggaaa
     3241 gaacggacgg tatcgttcac ttataaccaa tacgctcaga tgatgaacat cagtagggaa
     3301 aatgcttatg gtgtattagc taaagcaacc agagagctga tgacgagaac tgtggaaatc
     3361 aggaatcctt tggttaaagg ctttaagatt ttccagtgga caaactatgc caagttctca
     3421 agcgaaaaat tagaattagt ttttagtgaa gagatattgc cttatctttt ccagttaaaa
     3481 aaattcataa aatataatct ggaacatgtt aagtcttttg aaaacaaata ctctatgagg
     3541 atttatgagt ggttattaaa agaactaaca caaaagaaaa ctcacaaggc aaatatagag
     3601 attagccttg atgaatttaa gttcatgtta atgcttgaaa ataactacca tgagtttaaa
     3661 aggcttaacc aatgggtttt gaaaccaata agtaaagatt taaacactta cagcaatatg
     3721 aaattggtgg ttgataagcg aggccgcccg actgatacgt tgattttcca agttgaacta
     3781 gatagacaaa tggatctcgt aaccgaactt gagaacaacc agataaaaat gaatggtgac
     3841 aaaataccaa caaccattac atcagattcc tacctacgta acggactaag aaaaacacta
     3901 cacgatgctt taactgcaaa aattcagctc accagttttg aggcaaaatt tttgagtgac
     3961 atgcaaagta agtatgatct caatggttcg ttctcatggc tcacgcaaaa acaacgaacc
     4021 acactagaga acatactggc taaatacgga aggatctgag gttcttatgg ctcttgtatc
     4081 tatcagtgaa gcatcaagac taacaaacaa aagtagaaca actgttcacc gttacatatc
     4141 aaagggaaaa ctgtccatat gcacagatga aaacggtgta aaaaagatag atacatcaga
     4201 gcttttacga gtttttggtg cattcaaagc tgttcaccat gaacagatcg acaatgtaac
     4261 agatgaacag catgtaacac ctaatagaac aggtgaaacc agtaaaacaa agcaactaga
     4321 acatgaaatt gaacacctga gacaacttgt tacagctcaa cagtcacaca tagacagcct
     4381 gaaacaggcg atgctgctta tcgaatcaaa gctgccgaca acacgggagc cagtgacgcc
     4441 tcccgtgggg aaaaaatcat ggcaattctg gaagaaatag cgctttcagc cggcaaaccg
     4501 gctgaagccg gatctgcgat tctgataaca aactagcaac accagaacag cccgtttgcg
     4561 ggcagcaaaa cccgtgggaa ttaattcccc tgctcgcgca ggctgggtgc caagctctcg
     4621 ggtaacatca aggcccgatc cttggagccc ttgccctccc gcacgatgat cgtgccgtga
     4681 tcgaaatcca gatccttgac ccgcagttgc aaaccctcac tgatccgcat gcccgttcca
     4741 tacagaagct gggcgaacaa acgatgctcg ccttccagaa aaccgaggat gcgaaccact
     4801 tcatccgggg tcagcaccac cggcaagcgc cgcgacggcc gaggtcttcc gatctcctga
     4861 agccagggca gatccgtgca cagcaccttg ccgtagaaga acagcaaggc cgccaatgcc
     4921 tgacgatgcg tggagaccga aaccttgcgc tcgttcgcca gccaggacag aaatgcctcg
     4981 acttcgctgc tgcccaaggt tgccgggtga cgcacaccgt ggaaacggat gaaggcacga
     5041 acccagtgga cataagcctg ttcggttcgt aagctgtaat gcaagtagcg tatgcgctca
     5101 cgcaactggt ccagaacctt gaccgaacgc agcggtggta acggcgcagt ggcggttttc
     5161 atggcttgtt atgactgttt ttttggggta cagtctatgc ctcgggcatc caagcagcaa
     5221 gcgcgttacg ccgtgggtcg atgtttgatg ttatggagca gcaacgatgt tacgcagcag
     5281 ggcagtcgcc ctaaaacaaa gttaaacatc atgagggaag cggtgatcgc cgaagtatcg
     5341 actcaactat cagaggtagt tggcgtcatc gagcgccatc tcgaaccgac gttgctggcc
     5401 gtacatttgt acggctccgc agtggatggc ggcctgaagc cacacagtga tattgatttg
     5461 ctggttacgg tgaccgtaag gcttgatgaa acaacgcggc gagctttgat caacgacctt
     5521 ttggaaactt cggcttcccc tggagagagc gagattctcc gcgctgtaga agtcaccatt
     5581 gttgtgcacg acgacatcat tccgtggcgt tatccagcta agcgcgaact gcaatttgga
     5641 gaatggcagc gcaatgacat tcttgcaggt atcttcgagc cagccacgat cgacattgat
     5701 ctggctatct tgctgacaaa agcaagagaa catagcgttg ccttggtagg tccagcggcg
     5761 gaggaactct ttgatccggt tcctgaacag gatctatttg aggcgctaaa tgaaacctta
     5821 acgctatgga actcgccgcc cgactgggct ggcgatgagc gaaatgtagt gcttacgttg
     5881 tcccgcattt ggtacagcgc agtaaccggc aaaatcgcgc cgaaggatgt cgctgccgac
     5941 tgggcaatgg agcgcctgcc ggcccagtat cagcccgtca tacttgaagc tagacaggct
     6001 tatcttggac aagaagaaga tcgcttggcc tcgcgcgcag atcagttgga agaatttgtc
     6061 cactacgtga aaggcgagat caccaaggta gtcggcaaat aatgtctaac aattcgttca
     6121 agccgacgcc gcttcgcggc gcggcttaac tcaagcgtta gatgcactaa gcacataatt
     6181 gctcacagcc aaactatcag gtcaagtctg cttttattat ttttaagcgt gcataataag
     6241 ccctacacaa attgggagat atatcatgaa aggctggctt tttcttgtta tcgcaatagt
     6301 tggcgaagta atcgcaacat ccgca
//
