LOCUS       40924_37238        4105 bp DNA     circular SYN 18-DEC-2018
DEFINITION  Cloning vector pRLB1, complete sequence.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 4105)
  AUTHORS   Redder P, Linder P.
  TITLE     New Range of Vectors with a Stringent 5-Fluoroorotic Acid-Based 
            Counterselection System for Generating Mutants by Allelic 
            Replacement in Staphylococcus aureus
  JOURNAL   Appl. Environ. Microbiol. 78 (11), 3846-3854 (2012)
  PUBMED    22447609
REFERENCE   2  (bases 1 to 4105)
  AUTHORS   Redder P, Linder P.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2012) Department of Microbiology and Molecular 
            Medicine, University of Geneva, Faculty of Medicine, Rue 
            Michel-Servet 1, Geneva 1203, Switzerland
REFERENCE   3  (bases 1 to 4105)
  TITLE     Direct Submission
REFERENCE   4  (bases 1 to 4105)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Appl. 
            Environ. Microbiol."; date: "2012"; volume: "78"; issue: "11"; 
            pages: "3846-3854"
COMMENT     SGRef: number: 2; type: "Journal Article"; journalName: "Submitted 
            (12-MAR-2012) Department of Microbiology and Molecular Medicine, 
            University of Geneva, Faculty of Medicine, Rue Michel-Servet 1, 
            Geneva 1203, Switzerland"
COMMENT     SGRef: number: 3; type: "Journal Article"
COMMENT     ##Assembly-Data-START##
            Assembly Method       :: BioEdit, CAP v. 7.0.9.0
            Sequencing Technology :: Sanger dideoxy sequencing 
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..4105
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     misc_feature    complement(36..143)
                     /label=MCS
                     /note="pBluescript multiple cloning site"
     promoter        complement(152..170)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     rep_origin      619..841
                     /label=pSC101 ori
                     /note="low-copy replication origin that requires the Rep101
                     protein"
     CDS             889..1836
                     /codon_start=1
                     /label=Rep101
                     /note="RepA protein needed for replication with the pSC101 
                     origin"
                     /translation="MSELVVFKANELAISRYDLTEHETKLILCCVALLNPTIENPTRKE
                     RTVSFTYNQYAQMMNISRENAYGVLAKATRELMTRTVEIRNPLVKGFKIFQWTNYAKFS
                     SEKLELVFSEEILPYLFQLKKFIKYNLEHVKSFENKYSMRIYEWLLKELTQKKTHKANI
                     EISLDEFKFMLMLENNYHEFKRLNQWVLKPISKDLNTYSNMKLVVDKRGRPTDTLIFQV
                     ELDRQMDLVTELENNQIKMNGDKIPTTITSDSYLRNGLRKTLHDALTAKIQLTSFEAKF
                     LSDMQSKYDLNGSFSWLTQKQRTTLENILAKYGRI"
     CDS             3091..3879
                     /codon_start=1
                     /label=SmR
                     /note="aminoglycoside adenylyltransferase (Murphy, 1985)"
                     /translation="MREAVIAEVSTQLSEVVGVIERHLEPTLLAVHLYGSAVDGGLKPH
                     SDIDLLVTVTVRLDETTRRALINDLLETSASPGESEILRAVEVTIVVHDDIIPWRYPAK
                     RELQFGEWQRNDILAGIFEPATIDIDLAILLTKAREHSVALVGPAAEELFDPVPEQDLF
                     EALNETLTLWNSPPDWAGDERNVVLTLSRIWYSAVTGKIAPKDVAADWAMERLPAQYQP
                     VILEARQAYLGQEEDRLASRADQLEEFVHYVKGEITKVVGK"
ORIGIN
        1 ggcgcgccag atctcactaa agggaacaaa agctgggtac cgggcccccc ctcgaggtcg
       61 acggtatcga taagcttgat atcgaattcc tgcagcccgg gggatccact agttctagag
      121 cggccgccac cgcggtggag ctccaattcg ccctatagtg agtcgtatta ggcgcgcctt
      181 actgggtgca ttagccagtc tgaatgacct gtcacgggat aatccgaagt ggtcagactg
      241 gaaaatcaga gggcaggaac tgctgaacag caaaaagtca gatagcacca catagcagac
      301 ccgccataaa acgccctgag aagcccgtga cgggcttttc ttgtattatg ggtagtttcc
      361 ttgcatgaat ccataaaagg cgcctgtagt gccatttacc cccattcact gccagagccg
      421 tgagcgcagc gaactgaatg tcacgaaaaa gacagcgact caggtgcctg atggtcggag
      481 acaaaaggaa tattcagcga tttgcccgag cttgcgaggg tgctacttaa gcctttaggg
      541 ttttaaggtc tgttttgtag aggagcaaac agcgtttgcg acatcctttt gtaatactgc
      601 ggaactgact aaagtagtga gttatacaca gggctgggat ctattctttt tatctttttt
      661 tattctttct ttattctata aattataacc acttgaatat aaacaaaaaa aacacacaaa
      721 ggtctagcgg aatttacaga gggtctagca gaatttacaa gttttccagc aaaggtctag
      781 cagaatttac agatacccac aactcaaagg aaaaggacta gtaattatca ttgactagcc
      841 catctcaatt ggtatagtga ttaaaatcac ctagaccaat tgagatgtat gtctgaatta
      901 gttgttttca aagcaaatga actagcgatt agtcgctatg acttaacgga gcatgaaacc
      961 aagctaattt tatgctgtgt ggcactactc aaccccacga ttgaaaaccc tacaaggaaa
     1021 gaacggacgg tatcgttcac ttataaccaa tacgctcaga tgatgaacat cagtagggaa
     1081 aatgcttatg gtgtattagc taaagcaacc agagagctga tgacgagaac tgtggaaatc
     1141 aggaatcctt tggttaaagg ctttaagatt ttccagtgga caaactatgc caagttctca
     1201 agcgaaaaat tagaattagt ttttagtgaa gagatattgc cttatctttt ccagttaaaa
     1261 aaattcataa aatataatct ggaacatgtt aagtcttttg aaaacaaata ctctatgagg
     1321 atttatgagt ggttattaaa agaactaaca caaaagaaaa ctcacaaggc aaatatagag
     1381 attagccttg atgaatttaa gttcatgtta atgcttgaaa ataactacca tgagtttaaa
     1441 aggcttaacc aatgggtttt gaaaccaata agtaaagatt taaacactta cagcaatatg
     1501 aaattggtgg ttgataagcg aggccgcccg actgatacgt tgattttcca agttgaacta
     1561 gatagacaaa tggatctcgt aaccgaactt gagaacaacc agataaaaat gaatggtgac
     1621 aaaataccaa caaccattac atcagattcc tacctacgta acggactaag aaaaacacta
     1681 cacgatgctt taactgcaaa aattcagctc accagttttg aggcaaaatt tttgagtgac
     1741 atgcaaagta agtatgatct caatggttcg ttctcatggc tcacgcaaaa acaacgaacc
     1801 acactagaga acatactggc taaatacgga aggatctgag gttcttatgg ctcttgtatc
     1861 tatcagtgaa gcatcaagac taacaaacaa aagtagaaca actgttcacc gttacatatc
     1921 aaagggaaaa ctgtccatat gcacagatga aaacggtgta aaaaagatag atacatcaga
     1981 gcttttacga gtttttggtg cattcaaagc tgttcaccat gaacagatcg acaatgtaac
     2041 agatgaacag catgtaacac ctaatagaac aggtgaaacc agtaaaacaa agcaactaga
     2101 acatgaaatt gaacacctga gacaacttgt tacagctcaa cagtcacaca tagacagcct
     2161 gaaacaggcg atgctgctta tcgaatcaaa gctgccgaca acacgggagc cagtgacgcc
     2221 tcccgtgggg aaaaaatcat ggcaattctg gaagaaatag cgctttcagc cggcaaaccg
     2281 gctgaagccg gatctgcgat tctgataaca aactagcaac accagaacag cccgtttgcg
     2341 ggcagcaaaa cccgtgggaa ttaattcccc tgctcgcgca ggctgggtgc caagctctcg
     2401 ggtaacatca aggcccgatc cttggagccc ttgccctccc gcacgatgat cgtgccgtga
     2461 tcgaaatcca gatccttgac ccgcagttgc aaaccctcac tgatccgcat gcccgttcca
     2521 tacagaagct gggcgaacaa acgatgctcg ccttccagaa aaccgaggat gcgaaccact
     2581 tcatccgggg tcagcaccac cggcaagcgc cgcgacggcc gaggtcttcc gatctcctga
     2641 agccagggca gatccgtgca cagcaccttg ccgtagaaga acagcaaggc cgccaatgcc
     2701 tgacgatgcg tggagaccga aaccttgcgc tcgttcgcca gccaggacag aaatgcctcg
     2761 acttcgctgc tgcccaaggt tgccgggtga cgcacaccgt ggaaacggat gaaggcacga
     2821 acccagtgga cataagcctg ttcggttcgt aagctgtaat gcaagtagcg tatgcgctca
     2881 cgcaactggt ccagaacctt gaccgaacgc agcggtggta acggcgcagt ggcggttttc
     2941 atggcttgtt atgactgttt ttttggggta cagtctatgc ctcgggcatc caagcagcaa
     3001 gcgcgttacg ccgtgggtcg atgtttgatg ttatggagca gcaacgatgt tacgcagcag
     3061 ggcagtcgcc ctaaaacaaa gttaaacatc atgagggaag cggtgatcgc cgaagtatcg
     3121 actcaactat cagaggtagt tggcgtcatc gagcgccatc tcgaaccgac gttgctggcc
     3181 gtacatttgt acggctccgc agtggatggc ggcctgaagc cacacagtga tattgatttg
     3241 ctggttacgg tgaccgtaag gcttgatgaa acaacgcggc gagctttgat caacgacctt
     3301 ttggaaactt cggcttcccc tggagagagc gagattctcc gcgctgtaga agtcaccatt
     3361 gttgtgcacg acgacatcat tccgtggcgt tatccagcta agcgcgaact gcaatttgga
     3421 gaatggcagc gcaatgacat tcttgcaggt atcttcgagc cagccacgat cgacattgat
     3481 ctggctatct tgctgacaaa agcaagagaa catagcgttg ccttggtagg tccagcggcg
     3541 gaggaactct ttgatccggt tcctgaacag gatctatttg aggcgctaaa tgaaacctta
     3601 acgctatgga actcgccgcc cgactgggct ggcgatgagc gaaatgtagt gcttacgttg
     3661 tcccgcattt ggtacagcgc agtaaccggc aaaatcgcgc cgaaggatgt cgctgccgac
     3721 tgggcaatgg agcgcctgcc ggcccagtat cagcccgtca tacttgaagc tagacaggct
     3781 tatcttggac aagaagaaga tcgcttggcc tcgcgcgcag atcagttgga agaatttgtc
     3841 cactacgtga aaggcgagat caccaaggta gtcggcaaat aatgtctaac aattcgttca
     3901 agccgacgcc gcttcgcggc gcggcttaac tcaagcgtta gatgcactaa gcacataatt
     3961 gctcacagcc aaactatcag gtcaagtctg cttttattat ttttaagcgt gcataataag
     4021 ccctacacaa attgggagat atatcatgaa aggctggctt tttcttgtta tcgcaatagt
     4081 tggcgaagta atcgcaacat ccgca
//
