LOCUS       V003710                 7738 bp    DNA     circular SYN 18-DEC-2018
DEFINITION  Exported.
ACCESSION   V003710
VERSION     V003710
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
            .
REFERENCE   1  (bases 1 to 7738)
  AUTHORS   Koksharova OA, Wolk CP.
  TITLE     A novel gene that bears a DnaJ motif influences cyanobacterial cell
            division
  JOURNAL   J. Bacteriol. 184 (19), 5524-5528 (2002)
   PUBMED   12218043
REFERENCE   2  (bases 1 to 7738)
  AUTHORS   Wolk CP.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-SEP-2001) MSU-DOE Plant Research Laboratory, Michigan
            State University, Wilson Road, E. Lansing, MI 48824, USA
REFERENCE   3  (bases 1 to 7738)
  AUTHORS   Wolk CP.
  TITLE     Direct Submission
  JOURNAL   Submitted (01-MAR-2012) MSU-DOE Plant Research Laboratory, Michigan
            State University, Wilson Road, E. Lansing, MI 48824, USA
REFERENCE   4  (bases 1 to 7738)
  TITLE     Direct Submission
REFERENCE   5  (bases 1 to 7738)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "J.
            Bacteriol."; date: "2002"; volume: "184"; issue: "19"; pages:
            "5524-5528"
            SGRef: number: 2; type: "Journal Article"; journalName: "Submitted
            (26-SEP-2001) MSU-DOE Plant Research Laboratory, Michigan State
            University, Wilson Road, E. Lansing, MI 48824, USA"
            SGRef: number: 3; type: "Journal Article"; journalName: "Submitted
            (01-MAR-2012) MSU-DOE Plant Research Laboratory, Michigan State
            University, Wilson Road, E. Lansing, MI 48824, USA"
            SGRef: number: 4; type: "Journal Article"
            On Mar 1, 2012 this sequence version replaced AF424805.1.
            Transposon-bearing plasmid pRL692, a derivative of pRL1063a (GenBank
            Accession Number U55385), bearing transposon Tn5-692 containing the
            erm gene from pE194 (GenBank Accession Number J01755) and,
            downstream from the promoter of ribulose bisphosphate carboxylase
            from Synechococcus sp. PCC 6301 (GenBank Accession Number X03220),
            the aadA region from Tn7 (GenBank Accession Number X03043).  The
            transposon confers resistance to erythromycin, streptomycin and
            spectinomycin.  The transposase gene has been altered by
            substitution of a 208-bp Psp1406I fragment with an equal-sized
            fragment from pRZ5421 (Zhou, M., A. Bhasin, and W.S. Reznikoff, J.
            Mol. Biol. 276:913-925, 1998).  The inverted repeats are modeled on
            those of pRZ5421.  In addition, a SpeI site has been inserted
            between the promoter of the transposase and the nearby terminal
            inverted repeat of the transposon.
            Transposon Tn5-692 borne by pRL692 efficiently mutagenizes
            Synechococcus sp. strain PCC 7942 and Anabaena variabilis strain
            ATCC 29413.  The user is cautioned that this transposon appears able
            to transpose repeatedly.
FEATURES             Location/Qualifiers
     source          1..7738
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     misc_feature    1..19
                     /label="Tn5 ME"
                     /note="hyperactive mosaic end for Tn5 transposase
                     recognition (Reznikoff et al., 2004)"
     CDS             409..1197
                     /codon_start=1
                     /gene="aadA"
                     /product="3'(9)-O-nucleotidyltransferase"
                     /label="aadA"
                     /note="streptomycin adenylyltransferase; from Tn7"
                     /protein_id="AAL30370.1"
                     /translation="MREAVIAEVSTQLSEVVGVIERHLEPTLLAVHLYGSAVDGGLKPH
                     SDIDLLVTVTVRLDETTRRALINDLLETSASPGESEILRAVEVTIVVHDDIIPWRYPAK
                     RELQFGEWQRNDILAGIFEPATIDIDLAILLTKAREHSVALVGPAAEELFDPVPEQDLF
                     EALNETLTLWNSPPDWAGDERNVVLTLSRIWYSAVTGKIAPKDVAADWAMERLPAQYQP
                     VILEARQAYLGQEDRLASRADQLEEFVHYVKGEITKVVGK"
     gene            409..1197
                     /gene="aadA"
                     /label="aadA"
     rep_origin      2639..3227
                     /label="ori"
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
                     replication"
     CDS             complement(3481..4212)
                     /gene="ermC"
                     /label="rRNA adenine N-6-methyltransferase"
                     /note="rRNA adenine N-6-methyltransferase from
                     Staphylococcus aureus. Accession#: P02979"
     promoter        4485..4587
                     /label="cat promoter"
                     /note="promoter of the E. coli cat gene encoding
                     chloramphenicol acetyltransferase"
     CDS             complement(5350..6777)
                     /label="Tn5 transposase"
                     /note="transposase from the bacterial Tn5 transposon
                     (Reznikoff, 1993)"
     misc_feature    complement(6852..6870)
                     /label="Tn5 ME"
                     /note="hyperactive mosaic end for Tn5 transposase
                     recognition (Reznikoff et al., 2004)"
     CDS             complement(7056..7424)
                     /label="traJ"
                     /note="oriT-recognizing protein"
     oriT            complement(7457..7566)
                     /direction=LEFT
                     /label="oriT"
                     /note="incP origin of transfer"
ORIGIN
        1 ctgtctctta tacacatcta gcgtctagac ctagaggatc cgggtaccga gctcgaattc
       61 gagctcccca atcctcgtga tgatcagtga tggaaaaagc actgtaattc ccttggtttt
      121 tggctgaaag tttcggactc agtagaccta agtacagagt gatgtcaacg ccttcaagct
      181 agacgggagg cggcttttgc catggttcag cgatcgctcc tcatcttcaa taagcagggc
      241 atgagccagc gttaagcaaa tcaaatcaaa tctcgcttct gggcttcaat aaatggttcc
      301 gattgatgat aggttgattc atgcaagctt ggagcacagg atgacgccta acaattcatt
      361 caagccgaca ccgcttcgcg gcgcggctta attcaggagt taaacatcat gagggaagcg
      421 gtgatcgccg aagtatcgac tcaactatca gaggtagttg gcgtcatcga gcgccatctc
      481 gaaccgacgt tgctggccgt acatttgtac ggctccgcag tggatggcgg cctgaagcca
      541 cacagtgata ttgatttgct ggttacggtg accgtaaggc ttgatgaaac aacgcggcga
      601 gctttgatca acgacctttt ggaaacttcg gcttcccctg gagagagcga gattctccgc
      661 gctgtagaag tcaccattgt tgtgcacgac gacatcattc cgtggcgtta tccagctaag
      721 cgcgaactgc aatttggaga atggcagcgc aatgacattc ttgcaggtat cttcgagcca
      781 gccacgatcg acattgatct ggctatcttg ctgacaaaag caagagaaca tagcgttgcc
      841 ttggtaggtc cagcggcgga ggaactcttt gatccggttc ctgaacagga tctatttgag
      901 gcgctaaatg aaaccttaac gctatggaac tcgccgcccg actgggctgg cgatgagcga
      961 aatgtagtgc ttacgttgtc ccgcatttgg tacagcgcag taaccggcaa aatcgcgccg
     1021 aaggatgtcg ctgccgactg ggcaatggag cgcctgccgg cccagtatca gcccgtcata
     1081 cttgaagcta ggcaggctta tcttggacaa gaagatcgct tggcctcgcg cgcagatcag
     1141 ttggaagaat ttgttcacta cgtgaaaggc gagatcacca aggtagtcgg caaataatgt
     1201 ctaacaattc gttcaagccg acgccgcttc gcggcgcggc ttaactcaag cgttagagag
     1261 ctggggaaga ctatgcgcga tctgttgaag gtggttctaa gcctcgtact tgcgatggca
     1321 tcggggcagg cacttgctga cctgccaatt gttttagtgg atgaagctcg tcttccctat
     1381 gactactccc catccaacta cgacatttct ccaagcaact acgacaactc cataagcaat
     1441 tacgacaata gtccatcaaa ttacgacaac tctgagagca actacgataa tagttcatcc
     1501 aattacgaca atagtcgcaa cggaaatcgt aggcttatat atagcgcaaa tgggtctcgc
     1561 actttcgccg gctactacgt cattgccaac aatgggacaa cgaacttctt ttccacatct
     1621 ggcaaaagga tgttctacac cccaaaaggg gggcgcggcg tctatggcgg caaagatggg
     1681 agcttctgcg gggcattggt cgtcataaat ggccaatttt cgcttgccct gacagataac
     1741 ggcctgaaga tcatgtatct aagcaactag cctgctctct aataaaatgt taggcctcaa
     1801 catctagtcg caagctgagg ggaaccacta gtgtcatacg aacctccaag agacggttac
     1861 acaaacgggt acattgttga tgtcatgtat gacaatcgcc caagtaagta tccagctgtg
     1921 ttcagaacgt acgtccgaat tcgagctcgg tacccggatc ctctaggcct taagatcgaa
     1981 agagctggta gttggcgcac tgttcgaaga actgccgatg tccagtaaga ttcttactat
     2041 gctggttgaa ccggatgctg gtaaagctac ttgggttgct gcttctactt atggtaccga
     2101 tacaactact ggtgaggaag ttaaaggagc tcgcgaaagc ttgcatgcct gcagcaatgg
     2161 caacaacgtt gcgcaaacta ttaactggcg aactacttac tctagcttcc cggcaacaat
     2221 taatagactg gatggaggcg gataaagttg caggaccact tctgcgctcg gcccttccgg
     2281 ctggctggtt tattgctgat aaatctggag ccggtgagcg tgggtctcgc ggtatcattg
     2341 cagcactggg gccagatggt aagccctccc gtatcgtagt tatctacacg acggggagtc
     2401 aggcaactat ggatgaacga aatagacaga tcgctgagat aggtgcctca ctgattaagc
     2461 attggtaact gtcagaccaa gtttactcat atatacttta gattgattta aaacttcatt
     2521 tttaatttaa aaggatctag gtgaagatcc tttttgataa tctcatgacc aaaatccctt
     2581 aacgtgagtt ttcgttccac tgagcgtcag accccgtaga aaagatcaaa ggatcttctt
     2641 gagatccttt ttttctgcgc gtaatctgct gcttgcaaac aaaaaaacca ccgctaccag
     2701 cggtggtttg tttgccggat caagagctac caactctttt tccgaaggta actggcttca
     2761 gcagagcgca gataccaaat actgtccttc tagtgtagcc gtagttaggc caccacttca
     2821 agaactctgt agcaccgcct acatacctcg ctctgctaat cctgttacca gtggctgctg
     2881 ccagtggcga taagtcgtgt cttaccgggt tggactcaag acgatagtta ccggataagg
     2941 cgcagcggtc gggctgaacg gggggttcgt gcacacagcc cagcttggag cgaacgacct
     3001 acaccgaact gagataccta cagcgtgagc attgagaaag cgccacgctt cccgaaggga
     3061 gaaaggcgga caggtatccg gtaagcggca gggtcggaac aggagagcgc acgagggagc
     3121 ttccaggggg aaacgcctgg tatctttata gtcctgtcgg gtttcgccac ctctgacttg
     3181 agcgtcgatt tttgtgatgc tcgtcagggg ggcggagcct atggaaaaac gccagcaacg
     3241 cggccttttt acggttcctg gccttttgct ggccttttgc tcacatgttc tttcctgcgt
     3301 tatcccctga ttctgtggat aaccgtatta ccgcctttga gtgagctgat accgctcgcc
     3361 gcagccgaac gaccgagcgc agcgagtcag tgagcgagga agcggaagag cgcctgatgc
     3421 ggtattttct ccttacgcat ctgtgcggta tttcacaccg cacctgcatc ccttaactta
     3481 cttattaaat aatttatagc tattgaaaag agataagaat tgttcaaagc taatattgtt
     3541 taaatcgtca attcctgcat gttttaagga attgttaaat tgattttttg taaatatttt
     3601 cttgtattct ttgttaaccc atttcataac gaaataatta tacttttgtt tatctttgtg
     3661 tgatattctt gatttttttc tacttaatct gataagtgag ctattcactt taggtttagg
     3721 atgaaaatat tctcttggaa ccatacttaa tatagaaata tcaacttctg ccattaaaag
     3781 taatgccaat gagcgttttg tatttaataa tcttttagca aacccgtatt ccacgattaa
     3841 ataaatctca ttagctatac tatcaaaaac aattttgcgt attatatccg tacttatgtt
     3901 ataaggtata ttaccatata ttttatagga ttggttttta ggaaatttaa actgcaatat
     3961 atccttgttt aaaacttgga aattatcgtg atcaacaagt ttattttctg tagttttgca
     4021 taatttatgg tctatttcaa tggcagttac gaaattacac ctctttacta attcaagggt
     4081 aaaatggcct tttcctgagc cgatttcaaa gatattatca tgttcattta atcttatatt
     4141 tgtcattatt ttatctatat tatgttttga agtaataaag ttttgactgt gttttatatt
     4201 tttctcgttc attataaccc tctttaattt ggttatatga attttgctta ttaacgattc
     4261 attataacca cttatttttt gtttggttga taatgaactg tgctgattac aaaaatacta
     4321 aaaatgccca tattttttcc tccttataaa attagtataa ttatagcacg cgaattcatc
     4381 gaataaatac ctgtgacgga agatcacttc gcagaataaa taaatcctgg tgtccctgtt
     4441 gataccggga agccctgggc caacttttgg cgaaaatgag acgttgatcg gcacgtaaga
     4501 ggttccaact ttcaccataa tgaaataaga tcactaccgg gcgtattttt tgagttatcg
     4561 agattttcag gagctaagga agctaaaatg gagaaaaaaa tcactggata taccaccgtt
     4621 gatatatccc aatggcatcg taaagaacat tttgaggcat ttcagtcagt tgctcaatgt
     4681 acctataacc agaccgttca gctggatatt acggcctttt taaagaccgt aaagaaaaat
     4741 aagcacaagt tttatccggc ctttattcac attcttgccc gcctgatgaa tgctcatccg
     4801 gccggaattc cgtatggcaa tgaaagacgg tgagctggtg atatgggata gtgttcaccc
     4861 ttgttacacc gttttccatg agcaaactga aacgttttca tcgctctgga gtgaatacca
     4921 cgacgatttc cggcagtttc tacacatata ttcgcaagat gtggcgtgtt acggtgaaaa
     4981 cctggcctat ttccctaaag ggtttattga gaatatgttt ttcgtctcag ccaatccctg
     5041 ggtgagtttc accagttttg atttaaacgt ggccaatatg gacaacttct tcgcccccgt
     5101 tttcaccatg ggcaaatatt atacgcaagg cgacaaggtg ctgatgccgc tggcgattca
     5161 ggttcatcat gccgtttgtg atggcttcca tgtcggcaga atgcttaatg aattacaaca
     5221 gtactgcgat gagtggcagg gcggggcgta atttttttaa ggcagttatt ggtgccctta
     5281 aacgcctggt gctacgcctg aataagtgat aataagcgga tgaatggcag aaattcgata
     5341 tctagatcag atcttgatcc cctgcgccat cagatccttg gcggcaagaa agccatccag
     5401 tttactttgc agggcttccc aaccttccca gagggcgccc cagctggcaa ttccggttcg
     5461 cttgctgtcc ataaaaccgc ccagtctagc tatcgccatg taagcccact gcaagctacc
     5521 tgctttctct ttgcgcttgc gttttccctt gtccagatag cccagtagct gacattcatc
     5581 cggggtcagc accgtttctg cggactggct ttctacgtgt tccgcttcct ttagcagccc
     5641 ttgcgccctg agtgcttgcg gcagcgtgaa gctttctctg agctgtaaca gcctgaccgc
     5701 aacaaacgag aggatcgaga ccatccgctc cagattatcc ggctcctcca tgcgttgcct
     5761 ctcggctcct gctccggttt tccatgcctt atggaactcc tcgatccgcc agcgatgggt
     5821 ataaatgtcg atgacgcgca aggcttgggc tagcgactcg accggttcgc cggtcagcaa
     5881 caaccatttc aacggggtct cacccttggg cgggttaatc tcctcggcca gcaccgcgtt
     5941 gagcgtgata ttcccctgtt ttagcgtgat gcgcccactg cgcaggctca agctcgcctt
     6001 gcgggctggt cgatttttac gtttaccgcg tttatccacc acgccctttt gcggaatgct
     6061 gatctgatag ccacccaact ccggttggtt cttcagatgg tcgatcagat acaacccaga
     6121 ctctacgtcc ttgcgtgggt gcttggagcg caccacgaag cgctcgttat gcgccagcct
     6181 gtcctgcaga taagcatgaa tatcggcttc gcggtcacag accgcaatca cgttgctcat
     6241 catgctgccc atgcgtaacc ggctagttgc ggccgctgcc agccatttgc cactctcctt
     6301 ttcatccgca tcggcagggt catccgggcg catccaccac tcctgatgca gtaatcctac
     6361 ggtgcggaat gtggtggcct cgagcaagag aacggagtga acccaccatc cgcgggattt
     6421 atcctgaata gagcccagct tgccaagctc ttcggcgacc tggtggcgat aactcaaaga
     6481 ggtggtgtcc tcaatggcca gcagttcggg aaactcctga gccaacttga ctgtttgcat
     6541 ggcgccagcc tttctgatcg cctcggcaga aacgttggga ttgcggataa atcggtaagc
     6601 gccttcctgg gcggctttac taccctctga tgagatggtt attgatttac cagaatattt
     6661 tgccaattgg gcggcgacgt taaccaagcg ggcagtacgg cgaggatcac ccagcgccgc
     6721 cgaagagaac acagatttag cccagtcggc cgcacgatga agagcagaag ttatcatgaa
     6781 cgttaccatg ttaggaggtc acatggaata tcagattctg gaaaacggga aaggttccgt
     6841 actagtacgc tagatgtgta taagagacag ctgagctcgc gaaagcttgc atgcctgcag
     6901 gtcccctcga tgaattcatc gatgggatcg tcttgccttg ctcgtcggtg atgtacttca
     6961 ccagctccgc gaagtcgctc ttcttgatgg agcgcatggg gacgtgcttg gcaatcacgc
     7021 gcaccccccg gccgttttag cggctaaaaa agtcatggct ctgccctcgg gcggaccacg
     7081 cccatcatga ccttgccaag ctcgtcctgc ttctcttcga tcttcgccag cagggcgagg
     7141 atcgtggcat caccgaaccg cgccgtgcgc gggtcgtcgg tgagccagag tttcagcagg
     7201 ccgcccaggc ggcccaggtc gccattgatg cgggccagct cgcggacgtg ctcatagtcc
     7261 acgacgcccg tgattttgta gccctggccg acggccagca ggtaggccga caggctcatg
     7321 ccggccgccg ccgccttttc ctcaatcgct cttcgttcgt ctggaaggca gtacaccttg
     7381 ataggtgggc tgcccttcct ggttggcttg gtttcatcag ccatccgctt gccctcatct
     7441 gttacgccgg cggtagccgg ccagcctcgc agagcaggat tcccgttgag caccgccagg
     7501 tgcgaataag ggacagtgaa gaaggaacac ccgctcgcgg gtgggcctac ttcacctatc
     7561 ctgcccggct gacgccgttg gatacaccaa ggaaagtcta cacgaaccct ttggcaaaat
     7621 cctgtatatc gtgcgaaaaa ggatggatat accgaaaaaa tcgctataat gaccccgaag
     7681 cagggttatg cagcggaaga tcgtcgacct gcaggcatgc aagctttcgc gagctcag
//
