LOCUS       62056_18940        3495 bp DNA     circular SYN 01-JAN-1980
DEFINITION  synthetic circular DNA.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 3495)
  AUTHORS   .
  TITLE     Direct Submission
FEATURES             Location/Qualifiers
     source          1..3495
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     rep_origin      1..589
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     promoter        669..865
                     /label=SV40 promoter
                     /note="SV40 early promoter"
     intron          935..1067
                     /label=chimeric intron
                     /note="chimera between introns from human beta-globin and 
                     immunoglobulin heavy chain genes"
     promoter        1112..1130
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     CDS             1140..2072
                     /codon_start=1
                     /label=Rluc
                     /note="luciferase from the anthozoan coelenterate Renilla 
                     reniformis (sea pansy)"
                     /translation="MTSKVYDPEQRKRMITGPQWWARCKQMNVLDSFINYYDSEKHAEN
                     AVIFLHGNAASSYLWRHVVPHIEPVARCIIPDLIGMGKSGKSGNGSYRLLDHYKYLTAW
                     FELLNLPKKIIFVGHDWGACLAFHYSYEHQDKIKAIVHAESVVDVIESWDEWPDIEEDI
                     ALIKSEEGEKMVLENNFFVETMLPSKIMRKLEPEEFAAYLEPFKEKGEVRRPTLSWPRE
                     IPLVKGGKPDVVQIVRNYNAYLRASDDLPKMFIESDPGFFSNAIVEGAKKFPNTEFVKV
                     KGLHFSQEDAPDEMGKYIKSFVERVLKNEQ"
     polyA_signal    complement(2106..2227)
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     promoter        2360..2464
                     /label=AmpR promoter
     CDS             2465..3322
                     /codon_start=1
                     /label=AmpR
                     /note="beta-lactamase"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
ORIGIN
        1 ttgagatcct ttttttctgc gcgtaatctg ctgcttgcaa acaaaaaaac caccgctacc
       61 agcggtggtt tgtttgccgg atcaagagct accaactctt tttccgaagg taactggctt
      121 cagcagagcg cagataccaa atactgttct tctagtgtag ccgtagttag gccaccactt
      181 caagaactct gtagcaccgc ctacatacct cgctctgcta atcctgttac cagtggctgc
      241 tgccagtggc gataagtcgt gtcttaccgg gttggactca agacgatagt taccggataa
      301 ggcgcagcgg tcgggctgaa cggggggttc gtgcacacag cccagcttgg agcgaacgac
      361 ctacaccgaa ctgagatacc tacagcgtga gctatgagaa agcgccacgc ttcccgaagg
      421 gagaaaggcg gacaggtatc cggtaagcgg cagggtcgga acaggagagc gcacgaggga
      481 gcttccaggg ggaaacgcct ggtatcttta tagtcctgtc gggtttcgcc acctctgact
      541 tgagcgtcga tttttgtgat gctcgtcagg ggggcggagc ctatggaaaa acgccagcaa
      601 cgcggccttt ttacggttcc tggccttttg ctggcctttt gctcacatgg ctcgacagat
      661 ctgcgatctg catctcaatt agtcagcaac catagtcccg cccctaactc cgcccatccc
      721 gcccctaact ccgcccagtt ccgcccattc tccgccccat cgctgactaa ttttttttat
      781 ttatgcagag gccgaggccg cctcggcctc tgagctattc cagaagtagt gaggaggctt
      841 ttttggaggc ctaggctttt gcaaaaagct tgattcttct gacacaacag tctcgaactt
      901 aagctgcaga agttggtcgt gaggcactgg gcaggtaagt atcaaggtta caagacaggt
      961 ttaaggagac caatagaaac tgggcttgtc gagacagaga agactcttgc gtttctgata
     1021 ggcacctatt ggtcttactg acatccactt tgcctttctc tccacaggtg tccactccca
     1081 gttcaattac agctcttaag gctagagtac ttaatacgac tcactatagg ctagccacca
     1141 tgacttcgaa agtttatgat ccagaacaaa ggaaacggat gataactggt ccgcagtggt
     1201 gggccagatg taaacaaatg aatgttcttg attcatttat taattattat gattcagaaa
     1261 aacatgcaga aaatgctgtt atttttttac atggtaacgc ggcctcttct tatttatggc
     1321 gacatgttgt gccacatatt gagccagtag cgcggtgtat tataccagac cttattggta
     1381 tgggcaaatc aggcaaatct ggtaatggtt cttataggtt acttgatcat tacaaatatc
     1441 ttactgcatg gtttgaactt cttaatttac caaagaagat catttttgtc ggccatgatt
     1501 ggggtgcttg tttggcattt cattatagct atgagcatca agataagatc aaagcaatag
     1561 ttcacgctga aagtgtagta gatgtgattg aatcatggga tgaatggcct gatattgaag
     1621 aagatattgc gttgatcaaa tctgaagaag gagaaaaaat ggttttggag aataacttct
     1681 tcgtggaaac catgttgcca tcaaaaatca tgagaaagtt agaaccagaa gaatttgcag
     1741 catatcttga accattcaaa gagaaaggtg aagttcgtcg tccaacatta tcatggcctc
     1801 gtgaaatccc gttagtaaaa ggtggtaaac ctgacgttgt acaaattgtt aggaattata
     1861 atgcttatct acgtgcaagt gatgatttac caaaaatgtt tattgaatcg gacccaggat
     1921 tcttttccaa tgctattgtt gaaggtgcca agaagtttcc taatactgaa tttgtcaaag
     1981 taaaaggtct tcatttttcg caagaagatg cacctgatga aatgggaaaa tatatcaaat
     2041 cgttcgttga gcgagttctc aaaaatgaac aataattcta gagcggccgc ttcgagcaga
     2101 catgataaga tacattgatg agtttggaca aaccacaact agaatgcagt gaaaaaaatg
     2161 ctttatttgt gaaatttgtg atgctattgc tttatttgta accattataa gctgcaataa
     2221 acaagttaac aacaacaatt gcattcattt tatgtttcag gttcaggggg aggtgtggga
     2281 ggttttttaa agcaagtaaa acctctacaa atgtggtaaa atcgataagg atccaggtgg
     2341 cacttttcgg ggaaatgtgc gcggaacccc tatttgttta tttttctaaa tacattcaaa
     2401 tatgtatccg ctcatgagac aataaccctg ataaatgctt caataatatt gaaaaaggaa
     2461 gagtatgagt attcaacatt tccgtgtcgc ccttattccc ttttttgcgg cattttgcct
     2521 tcctgttttt gctcacccag aaacgctggt gaaagtaaaa gatgctgaag atcagttggg
     2581 tgcacgagtg ggttacatcg aactggatct caacagcggt aagatccttg agagttttcg
     2641 ccccgaagaa cgttttccaa tgatgagcac ttttaaagtt ctgctatgtg gcgcggtatt
     2701 atcccgtatt gacgccgggc aagagcaact cggtcgccgc atacactatt ctcagaatga
     2761 cttggttgag tactcaccag tcacagaaaa gcatcttacg gatggcatga cagtaagaga
     2821 attatgcagt gctgccataa ccatgagtga taacactgcg gccaacttac ttctgacaac
     2881 gatcggagga ccgaaggagc taaccgcttt tttgcacaac atgggggatc atgtaactcg
     2941 ccttgatcgt tgggaaccgg agctgaatga agccatacca aacgacgagc gtgacaccac
     3001 gatgcctgta gcaatggcaa caacgttgcg caaactatta actggcgaac tacttactct
     3061 agcttcccgg caacaattaa tagactggat ggaggcggat aaagttgcag gaccacttct
     3121 gcgctcggcc cttccggctg gctggtttat tgctgataaa tctggagccg gtgagcgtgg
     3181 gtctcgcggt atcattgcag cactggggcc agatggtaag ccctcccgta tcgtagttat
     3241 ctacacgacg gggagtcagg caactatgga tgaacgaaat agacagatcg ctgagatagg
     3301 tgcctcactg attaagcatt ggtaactgtc agaccaagtt tactcatata tactttagat
     3361 tgatttaaaa cttcattttt aatttaaaag gatctaggtg aagatccttt ttgataatct
     3421 catgaccaaa atcccttaac gtgagttttc gttccactga gcgtcagacc ccgtagaaaa
     3481 gatcaaagga tcttc
//
