LOCUS       40924_37178        7482 bp DNA     circular SYN 14-DEC-2018
DEFINITION  broad-host-range expression vector pRKNH3, complete sequence.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 7482)
  AUTHORS   Hulter N, Wackernagel W.
  TITLE     Double illegitimate recombination events integrate DNA segments 
            through two different mechanisms during natural transformation of 
            Acinetobacter baylyi
  JOURNAL   Mol. Microbiol. 67 (5), 984-995 (2008)
  PUBMED    18194157
REFERENCE   2  (bases 1 to 7482)
  AUTHORS   Huelter N, Wackernagel W.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-MAY-2007) Genetics, Department of Biology and 
            Environmental Sciences, Carl von Ossietzky University Oldenburg, 
            Carl-von-Ossietzky-Strasse 9-11, Oldenburg 26111, Germany
REFERENCE   3  (bases 1 to 7482)
  TITLE     Direct Submission
REFERENCE   4  (bases 1 to 7482)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Mol. 
            Microbiol."; date: "2008"; volume: "67"; issue: "5"; pages: 
            "984-995"
COMMENT     SGRef: number: 2; type: "Journal Article"; journalName: "Submitted 
            (21-MAY-2007) Genetics, Department of Biology and Environmental 
            Sciences, Carl von Ossietzky University Oldenburg, 
            Carl-von-Ossietzky-Strasse 9-11, Oldenburg 26111, Germany"
COMMENT     SGRef: number: 3; type: "Journal Article"
FEATURES             Location/Qualifiers
     source          1..7482
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     rep_origin      complement(1..712)
                     /direction=LEFT
                     /label=oriV
                     /note="incP origin of replication"
     misc_feature    714..733
                     /label=multiple cloning site
                     /note="multiple cloning site"
     CDS             complement(836..1483)
                     /codon_start=1
                     /label=TetR
                     /note="tetracycline resistance regulatory protein"
                     /translation="MTKLQPNTVIRAALDLLNEVGVDGLTTRKLAERLGVQQPALYWHF
                     RNKRALLDALAEAMLAENHTHSVPRADDDWRSFLIGNARSFRQALLAYRDGARIHAGTR
                     PGAPQMETADAQLRFLCEAGFSAGDAVNALMTISYFTVGAVLEEQAGDSDAGERGGTVE
                     QAPLSPLLRAAIDAFDEAGPDAAFEQGLAVIVDGLAKRRLVVRNVEGPRKGDD"
     CDS             1589..2785
                     /codon_start=1
                     /label=TcR
                     /note="tetracycline efflux protein"
                     /translation="MKPNIPLIVILSTVALDAVGIGLIMPVLPGLLRDLVHSNDVTAHY
                     GILLALYALVQFACAPVLGALSDRFGRRPILLVSLAGATVDYAIMATAPFLWVLYIGRI
                     VAGITGATGAVAGAYIADITDGDERARHFGFMSACFGFGMVAGPVLGGLMGGFSPHAPF
                     FAAAALNGLNFLTGCFLLPESHKGERRPLRREALNPLASFRWARGMTVVAALMAVFFIM
                     QLVGQVPAALWVIFGEDRFHWDATTIGISLAAFGILHSLAQAMITGPVAARLGERRALM
                     LGMIADGTGYILLAFATRGWMAFPIMVLLASGGIGMPALQAMLSRQVDEERQGQLQGSL
                     AALTSLTSIVGPLLFTAIYAASITTWNGWAWIAGAALYLLCLPALRRGLWSGAGQRADR
                     "
     CDS             complement(2869..4014)
                     /codon_start=1
                     /label=trfA
                     /note="trans-acting replication protein that binds to and 
                     activates oriV"
                     /translation="MNRTFDRKAYRQELIDAGFSAEDAETIASRTVMRAPRETFQSVGS
                     MVQQATAKIERDSVQLAPPALPAPSAAVERSRRLEQEAAGLAKSMTIDTRGTMTTKKRK
                     TAGEDLAKQVSEAKQAALLKHTKQQIKEMQLSLFDIAPWPDTMRAMPNDTARSALFTTR
                     NKKIPREALQNKVIFHVNKDVKITYTGVELRADDDELVWQQVLEYAKRTPIGEPITFTF
                     YELCQDLGWSINGRYYTKAEECLSRLQATAMGFTSDRVGHLESVSLLHRFRVLDRGKKT
                     SRCQVLIDEEIVVLFAGDHYTKFIWEKYRKLSPTARRMFDYFSSHREPYPLKLETFRLM
                     CGSDSTRVKKWREQVGEACEELRGSGLVEHAWVNDDLVHCKR"
     CDS             complement(4063..4413)
                     /codon_start=1
                     /gene="ssb"
                     /product="Ssb"
                     /label=ssb
                     /note="single-stranded DNA binding protein"
                     /protein_id="ABR10912.1"
                     /translation="MSHNQFQFIGNLTRDTEVRHGNSNKPQAIFDIAVNEEWRNDAGDK
                     QERTDFFRIKCFGSQAEAHGKYLGKGSLVFVQGKIRNTKYEKDGQTVYGTDFIADKVDY
                     LDTKAPGGSNQE"
     gene            complement(4063..4413)
                     /gene="ssb"
                     /label=ssb
     CDS             4534..4899
                     /codon_start=1
                     /gene="trbA"
                     /product="TrbA"
                     /label=trbA
                     /note="regulation of tra gene expression"
                     /protein_id="ABR10913.1"
                     /translation="MYNQIFFTNILRLLDERGMTKHELSERAGVSISFLSDLTNGKANP
                     SLKVMEAIADALETPLPLLLESTDLDREALAEIAGHPFKSSVPPGYERISVVLPSHKAF
                     IVKKWGDDTRKKLRGRL"
     gene            4534..4899
                     /gene="trbA"
                     /label=trbA
     CDS             complement(5937..7031)
                     /codon_start=1
                     /gene="incC1"
                     /product="IncC1"
                     /label=incC1
                     /note="IncC partitioning protein, long form"
                     /protein_id="ABR10914.1"
                     /translation="MGVIHEETAYRKPVPGGDPGAGSGAADHRDSAGRLSRWEATGDVR
                     NVAGTDQGRSVASGASRVGRVRGQELARGVRAGNGGSAGTSGVHRPEVGSGRQEKTGNQ
                     TMKTLVTANQKGGVGKTSTLVHLAFDFFERGLRVAVIDLDPQGNASYTLKDFATGLHAS
                     KLFGAVPAGGWTETAPAAGDGQAARLALIESNPVLANAERLSLDDARELFGANIKALAN
                     QGFDVCLIDTAPTLGVGLAAALFAADYVLSPIELEAYSIQGIKKMVTTIANVRQKNAKL
                     QFLGMVPSKVDARNPRHARHQAELLAAYPKMMIPATVGLRSSIADALASGVPVWKIKKT
                     AARKASKEVRALADYVFTKMEISQ"
     gene            complement(5937..7031)
                     /gene="incC1"
                     /label=incC1
     CDS             complement(5937..6716)
                     /codon_start=1
                     /gene="incC2"
                     /product="IncC2"
                     /label=incC2
                     /note="IncC partitioning protein, short form"
                     /protein_id="ABR10915.1"
                     /translation="MKTLVTANQKGGVGKTSTLVHLAFDFFERGLRVAVIDLDPQGNAS
                     YTLKDFATGLHASKLFGAVPAGGWTETAPAAGDGQAARLALIESNPVLANAERLSLDDA
                     RELFGANIKALANQGFDVCLIDTAPTLGVGLAAALFAADYVLSPIELEAYSIQGIKKMV
                     TTIANVRQKNAKLQFLGMVPSKVDARNPRHARHQAELLAAYPKMMIPATVGLRSSIADA
                     LASGVPVWKIKKTAARKASKEVRALADYVFTKMEISQ"
     gene            complement(5937..6716)
                     /gene="incC2"
                     /label=incC2
     CDS             complement(6713..7018)
                     /codon_start=1
                     /gene="korA"
                     /product="KorA"
                     /label=korA
                     /note="regulation of gene expression"
                     /protein_id="ABR10916.1"
                     /translation="MKKRLTESQFQEAIQGLEVGQQTIEIARGVLVDGKPQATFATSLG
                     LTRGAVSQAVHRVWAAFEDKNLPEGYARVTAVLPEHQAYIVRKWEADAKKKQETKR"
     gene            complement(6713..7018)
                     /gene="korA"
                     /label=korA
ORIGIN
        1 ctgccatttt tggggtgagg ccgttcgcgg ccgaggggcg cagcccctgg ggggatggga
       61 ggcccgcgtt agcgggccgg gagggttcga gaaggggggg cacccccctt cggcgtgcgc
      121 ggtcacgcgc acagggcgca gccctggtta aaaacaaggt ttataaatat tggtttaaaa
      181 gcaggttaaa agacaggtta gcggtggccg aaaaacgggc ggaaaccctt gcaaatgctg
      241 gattttctgc ctgtggacag cccctcaaat gtcaataggt gcgcccctca tctgtcagca
      301 ctctgcccct caagtgtcaa ggatcgcgcc cctcatctgt cagtagtcgc gcccctcaag
      361 tgtcaatacc gcagggcact tatccccagg cttgtccaca tcatctgtgg gaaactcgcg
      421 taaaatcagg cgttttcgcc gatttgcgag gctggccagc tccacgtcgc cggccgaaat
      481 cgagcctgcc cctcatctgt caacgccgcg ccgggtgagt cggcccctca agtgtcaacg
      541 tccgcccctc atctgtcagt gagggccaag ttttccgcga ggtatccaca acgccggcgg
      601 ccgcggtgtc tcgcacacgg cttcgacggc gtttctggcg cgtttgcagg gccatagacg
      661 gccgccagcc cagcggcgag ggcaaccagc ccggtgagcg tcggaaaggc gctatgcatg
      721 gatccgcccg ggcgatatcg ctacgggcgt ctgacgcggt ggaaaggggg aggggatgtt
      781 gtctacatgg ctctgctgta gtgagtgggt tgcgctccgg cagcggtcct gatcaatcgt
      841 caccctttct cggtccttca acgttcctga caacgagcct ccttttcgcc aatccatcga
      901 caatcaccgc gagtccctgc tcgaacgctg cgtccggacc ggcttcgtcg aaggcgtcta
      961 tcgcggcccg caacagcggc gagagcggag cctgttcaac ggtgccgccg cgctcgccgg
     1021 catcgctgtc gccggcctgc tcctcaagca cggccccaac agtgaagtag ctgattgtca
     1081 tcagcgcatt gacggcgtcc ccggccgaaa aacccgcctc gcagaggaag cgaagctgcg
     1141 cgtcggccgt ttccatctgc ggtgcgcccg gtcgcgtgcc ggcatggatg cgcgcgccat
     1201 cgcggtaggc gagcagcgcc tgcctgaagc tgcgggcatt cccgatcaga aatgagcgcc
     1261 agtcgtcgtc ggctctcggc accgaatgcg tatgattctc cgccagcatg gcttcggcca
     1321 gtgcgtcgag cagcgcccgc ttgttcctga agtgccagta aagcgccggc tgctgaaccc
     1381 ccaaccgttc cgccagtttg cgtgtcgtca gaccgtctac gccgacctcg ttcaacaggt
     1441 ccagggcggc acggatcact gtattcggct gcaactttgt catgcttgac actttatcac
     1501 tgataaacat aatatgtcca ccaacttatc agtgataaag aatccgcgcg ttcaatcgga
     1561 ccagcggagg ctggtccgga ggccagacat gaaacccaac atacccctga tcgtaattct
     1621 gagcactgtc gcgctcgacg ctgtcggcat cggcctgatt atgccggtgc tgccgggcct
     1681 cctgcgcgat ctggttcact cgaacgacgt caccgcccac tatggcattc tgctggcgct
     1741 gtatgcgttg gtgcaatttg cctgcgcacc tgtgctgggc gcgctgtcgg atcgtttcgg
     1801 gcggcggcca atcttgctcg tctcgctggc cggcgccact gtcgactacg ccatcatggc
     1861 gacagcgcct ttcctttggg ttctctatat cgggcggatc gtggccggca tcaccggggc
     1921 gactggggcg gtagccggcg cttatattgc cgatatcact gatggcgatg agcgcgcgcg
     1981 gcacttcggc ttcatgagcg cctgtttcgg gttcgggatg gtcgcgggac ctgtgctcgg
     2041 tgggctgatg ggcggtttct ccccccacgc tccgttcttc gccgcggcag ccttgaacgg
     2101 cctcaatttc ctgacgggct gtttcctttt gccggagtcg cacaaaggcg aacgccggcc
     2161 gttacgccgg gaggctctca acccgctcgc ttcgttccgg tgggcccggg gcatgaccgt
     2221 cgtcgccgcc ctgatggcgg tcttcttcat catgcaactt gtcggacagg tgccggccgc
     2281 gctttgggtc attttcggcg aggatcgctt tcactgggac gcgaccacga tcggcatttc
     2341 gcttgccgca tttggcattc tgcattcact cgcccaggca atgatcaccg gccctgtagc
     2401 cgcccggctc ggcgaaaggc gggcactcat gctcggaatg attgccgacg gcacaggcta
     2461 catcctgctt gccttcgcga cacggggatg gatggcgttc ccgatcatgg tcctgcttgc
     2521 ttcgggtggc atcggaatgc cggcgctgca agcaatgttg tccaggcagg tggatgagga
     2581 acgtcagggg cagctgcaag gctcactggc ggcgctcacc agcctgacct cgatcgtcgg
     2641 acccctcctc ttcacggcga tctatgcggc ttctataaca acgtggaacg ggtgggcatg
     2701 gattgcaggc gctgccctct acttgctctg cctgccggcg ctgcgtcgcg ggctttggag
     2761 cggcgcaggg caacgagccg atcgctgatc gtggaaacga taggcctgaa atgccagtaa
     2821 agcgctggct gctgaacccc cagccggaac tgaccccaca aggccctagc gtttgcaatg
     2881 caccaggtca tcattgaccc aggcgtgttc caccaggccg ctgcctcgca actcttcgca
     2941 ggcttcgccg acctgctcgc gccacttctt tacgcgggtg gaatccgatc cgcacatgag
     3001 gcggaaggtt tccagcttga gcgggtacgg ctcccggtgc gagctgaaat agtcgaacat
     3061 ccgtcgggcc gtcggcgaca gcttgcggta cttctcccat atgaatttcg tgtagtggtc
     3121 gccagcaaac agcacgacga tttcctcgtc gatcaggacc tggcaacggg acgttttctt
     3181 gccacggtcc aggacgcgga agcggtgcag cagcgacacc gattccaggt gcccaacgcg
     3241 gtcggacgtg aagcccatcg ccgtcgcctg taggcgcgac aggcattcct cggccttcgt
     3301 gtaataccgg ccattgatcg accagcccag gtcctggcaa agctcgtaga acgtgaaggt
     3361 gatcggctcg ccgatagggg tgcgcttcgc gtactccaac acctgctgcc acaccagttc
     3421 gtcatcgtcg gcccgcagct cgacgccggt gtaggtgatc ttcacgtcct tgttgacgtg
     3481 gaaaatgacc ttgttttgca gcgcctcgcg cgggattttc ttgttgcgcg tggtgaacag
     3541 ggcagagcgg gccgtgtcgt ttggcatcgc tcgcatcgtg tccggccacg gcgcaatatc
     3601 gaacaaggaa agctgcattt ccttgatctg ctgcttcgtg tgtttcagca acgcggcctg
     3661 cttggcctcg ctgacctgtt ttgccaggtc ctcgccggcg gtttttcgct tcttggtcgt
     3721 catagttcct cgcgtgtcga tggtcatcga cttcgccaaa cctgccgcct cctgttcgag
     3781 acgacgcgaa cgctccacgg cggccgatgg cgcgggcagg gcagggggag ccagttgcac
     3841 gctgtcgcgc tcgatcttgg ccgtagcttg ctggaccatc gagccgacgg actggaaggt
     3901 ttcgcggggc gcacgcatga cggtgcggct tgcgatggtt tcggcatcct cggcggaaaa
     3961 ccccgcgtcg atcagttctt gcctgtatgc cttccggtca aacgtccgat tcattcaccc
     4021 tccttgcggg attgccccga ctcacgccgg ggcaatgtgc ccttattcct gatttgaccc
     4081 gcctggtgcc ttggtgtcca gataatccac cttatcggca atgaagtcgg tcccgtagac
     4141 cgtctggccg tccttctcgt acttggtatt ccgaatcttg ccctgcacga ataccagcga
     4201 ccccttgccc aaatacttgc cgtgggcctc ggcctgagag ccaaaacact tgatgcggaa
     4261 gaagtcggtg cgctcctgct tgtcgccggc atcgttgcgc cactcttcat taaccgctat
     4321 atcgaaaatt gcttgcggct tgttagaatt gccatgacgt acctcggtgt cacgggtaag
     4381 attaccgata aactggaact gattatggct catatcgaaa gtctccttga gaaaggagac
     4441 tctagtttag ctaaacattg gttccgctgt caagaacttt agcggctaaa attttgcggg
     4501 ccgcgaccaa aggtgcgagg ggcggcttcc gctgtgtaca accagatatt tttcaccaac
     4561 atccttcgtc tgctcgatga gcggggcatg acgaaacatg agctgtcgga gagggcaggg
     4621 gtttcaattt cgtttttatc agacttaacc aacggtaagg ccaacccctc gttgaaggtg
     4681 atggaggcca ttgccgacgc cctggaaact cccctacctc ttctcctgga gtccaccgac
     4741 cttgaccgcg aggcactcgc ggagattgcg ggtcatcctt tcaagagcag cgtgccgccc
     4801 ggatacgaac gcatcagtgt ggttttgccg tcacataagg cgtttatcgt aaagaaatgg
     4861 ggcgacgaca cccgaaaaaa gctgcgtgga aggctctgac gccaagggtt agggcttgca
     4921 cttccttctt tagccgctaa aacggcccct tctctgcggg ccgtcggctc gcgcatcata
     4981 tcgacatcct caacggaagc cgtgccgcga atggcatcgg gcgggtgcgc tttgacagtt
     5041 gttttctatc agaaccccta cgtcgtgcgg ttcgattagc tgtttgtctt gcaggctaaa
     5101 cactttcggt atatcgtttg cctgtgcgat aatgttgcta atgatttgtt gcgtaggggt
     5161 tactgaaaag tgagcgggaa agaagagttt cagaccatca aggagcgggc caagcgcaag
     5221 ctggaacgcg acatgggtgc ggacctgttg gccgcgctca acgacccgaa aaccgttgaa
     5281 gtcatgctca acgcggacgg caaggtgtgg cacgaacgcc ttggcgagcc gatgcggtac
     5341 atctgcgaca tgcggcccag ccagtcgcag gcgattatag aaacggtggc cggattccac
     5401 ggcaaagagg tcacgcggca ttcgcccatc ctggaaggcg agttcccctt ggatggcagc
     5461 cgctttgccg gccaattgcc gccggtcgtg gccgcgccaa cctttgcgat ccgcaagcgc
     5521 gcggtcgcca tcttcacgct ggaacagtac gtcgaggcgg gcatcatgac ccgcgagcaa
     5581 tacgaggtca ttaaaagcgc cgtgattgat gatatagcgg cccggctgct cctggttctc
     5641 gcgcaccgaa atgggtgact tcaccccgcg ctctttgatc gtggcaccga tttccgcgat
     5701 gctctccggg gaaaagccgg ggttgtcggc cgtccgcggc tgatgcggat cttcgtcgat
     5761 caggtccagg tccagctcga tagggccgga accgccctga gacgccgcag gagcgtccag
     5821 gaggctcgac aggtcgccga tgctatccaa ccccaggccg gacggctgcg ccgcgcctgc
     5881 ggcttcctga gcggccgcag cggtgttttt cttggtggtc ttggcttgag ccgcagtcat
     5941 tgggaaatct ccatcttcgt gaacacgtaa tcagccaggg cgcgaacctc tttcgatgcc
     6001 ttgcgcgcgg ccgttttctt gatcttccag accggcacac cggatgcgag ggcatcggcg
     6061 atgctgctgc gcaggccaac ggtggccgga atcatcatct tggggtacgc ggccagcagc
     6121 tcggcttggt ggcgcgcgtg gcgcggattc cgcgcatcga ccttgctggg caccatgcca
     6181 aggaattgca gcttggcgtt cttctggcgc acgttcgcaa tggtcgtgac catcttcttg
     6241 atgccctgga tgctgtacgc ctcaagctcg atgggggaca gcacatagtc ggccgcgaag
     6301 agggcggccg ccaggccgac gccaagggtc ggggccgtgt cgatcaggca cacgtcgaag
     6361 ccttggttcg ccagggcctt gatgttcgcc ccgaacagct cgcgggcgtc gtccagcgac
     6421 agccgttcgg cgttcgccag taccgggttg gactcgatga gggcgaggcg cgcggcctgg
     6481 ccgtcgccgg ctgcgggtgc ggtttcggtc cagccgccgg cagggacagc gccgaacagc
     6541 ttgcttgcat gcaggccggt agcaaagtcc ttgagcgtgt aggacgcatt gccctggggg
     6601 tccaggtcga tcacggcaac ccgcaagccg cgctcgaaaa agtcgaaggc aagatgcaca
     6661 agggtcgaag tcttgccgac gccgcctttc tggttggccg tgaccaaagt tttcatcgtt
     6721 tggtttcctg ttttttcttg gcgtccgctt cccacttccg gacgatgtac gcctgatgtt
     6781 ccggcagaac cgccgttacc cgcgcgtacc cctcgggcaa gttcttgtcc tcgaacgcgg
     6841 cccacacgcg atgcaccgct tgcgacactg cgcccctggt cagtcccagc gacgttgcga
     6901 acgtcgcctg tggcttccca tcgactaaga cgccccgcgc tatctcgatg gtctgctgcc
     6961 ccacttccag cccctggatc gcctcctgga actggctttc ggtaagccgt ttcttcatgg
     7021 ataacaccca taatttgctc cgcgccttgg ttgaacatag cggtgacagc cgccagcaca
     7081 tgagagaagt ttagctaaac atttctcgca cgtcaacacc tttagccgct aaaactcgtc
     7141 cttggcgtaa caaaacaaaa gcccggaaac cgggctttcg tctcttgccg cttatggctc
     7201 tgcacccggc tccatcacca acaggtcgcg cacgcgcttc actcggttgc ggatcgacac
     7261 tgccagccca acaaagccgg ttgccgccgc cgccaggatc gcgccgatga tgccggccac
     7321 accggccatc gcccaccagg tcgccgccct cactgcccgg cacctggtcg ctgaatgtcg
     7381 atgccagcac ctgcggcacg tcaatgcttc cgggcgtcgc gctcgggctg atcgcccatc
     7441 ccgttactgc cccgatcccg gcaatggcaa ggactgccag cg
//
