LOCUS       40924_37168       11797 bp DNA     circular SYN 18-DEC-2018
DEFINITION  Cloning vector pRK7813, complete sequence.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 11797)
  AUTHORS   Jones JD, Gutterson N.
  TITLE     An efficient mobilizable cosmid vector, pRK7813, and its use in a 
            rapid method for marker exchange in Pseudomonas fluorescens strain 
            HV37a
  JOURNAL   Gene 61 (3), 299-306 (1987)
  PUBMED    2833429
REFERENCE   2  (bases 1 to 11797)
  AUTHORS   Cheng J, Charles TC.
  TITLE     Direct Submission
  JOURNAL   Submitted (17-DEC-2012) Department of Biology, University of 
            Waterloo, 200 University Avenue West, Waterloo, Ontario N2L 3G1, 
            Canada
REFERENCE   3  (bases 1 to 11797)
  TITLE     Direct Submission
REFERENCE   4  (bases 1 to 11797)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Gene"; 
            date: "1987"; volume: "61"; issue: "3"; pages: "299-306"
COMMENT     SGRef: number: 2; type: "Journal Article"; journalName: "Submitted 
            (17-DEC-2012) Department of Biology, University of Waterloo, 200 
            University Avenue West, Waterloo, Ontario N2L 3G1, Canada"
COMMENT     SGRef: number: 3; type: "Journal Article"
FEATURES             Location/Qualifiers
     source          1..11797
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     252..268
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(317..333)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     protein_bind    complement(341..357)
                     /label=lac operator
                     /note="The lac repressor binds to the lac operator to
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(365..395)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    complement(410..431)
                     /label=CAP binding site
                     /note="CAP binding activates transcription in the presence
                     of cAMP."
     CDS             complement(666..1034)
                     /codon_start=1
                     /label=traJ
                     /note="oriT-recognizing protein"
                     /translation="MADETKPTRKGSPPIKVYCLPDERRAIEEKAAAAGMSLSAYLLAV
                     GQGYKITGVVDYEHVRELARINGDLGRLGGLLKLWLTDDPRTARFGDATILALLAKIEE
                     KQDELGKVMMGVVRPRAEP"
     oriT            complement(1067..1176)
                     /direction=LEFT
                     /label=oriT
                     /note="incP origin of transfer"
     CDS             complement(1860..2507)
                     /codon_start=1
                     /label=TetR
                     /note="tetracycline resistance regulatory protein"
                     /translation="MTKLQPNTVIRAALDLLNEVGVDGLTTRKLAERLGVQQPALYWHF
                     RNKRALLDALAEAMLAENHTHSVPRADDDWRSFLIGNARSFRQALLAYRDGARIHAGTR
                     PGAPQMETADAQLRFLCEAGFSAGDAVNALMTISYFTVGAVLEEQAGDSDAGERGGTVE
                     QAPLSPLLRAAIDAFDEAGPDAAFEQGLAVIVDGLAKRRLVVRNVEGPRKGDD"
     CDS             2613..3809
                     /codon_start=1
                     /label=TcR
                     /note="tetracycline efflux protein"
                     /translation="MKPNIPLIVILSTVALDAVGIGLIMPVLPGLLRDLVHSNDVTAHY
                     GILLALYALVQFACAPVLGALSDRFGRRPILLVSLAGATVDYAIMATAPFLWVLYIGRI
                     VAGITGATGAVAGAYIADITDGDERARHFGFMSACFGFGMVAGPVLGGLMGGFSPHAPF
                     FAAAALNGLNFLTGCFLLPESHKGERRPLRREALNPLASFRWARGMTVVAALMAVFFIM
                     QLVGQVPAALWVIFGEDRFHWDATTIGISLAAFGILHSLAQAMITGPVAARLGERRALM
                     LGMIADGTGYILLAFATRGWMAFPIMVLLASGGIGMPALQAMLSRQVDEERQGQLQGSL
                     AALTSLTSIVGPLLFTAIYAASITTWNGWAWIAGAALYLLCLPALRRGLWSGAGQRADR
                     "
     misc_recomb     4180..4412
                     /label=lambda cos site
                     /note="lambda cos site"
     CDS             complement(6214..7359)
                     /codon_start=1
                     /label=trfA
                     /note="trans-acting replication protein that binds to and 
                     activates oriV"
                     /translation="MNRTFDRKAYRQELIDAGFSAEDAETIASRTVMRAPRETFQSVGS
                     MVQQATAKIERDSVQLAPPALPAPSAAVERSRRLEQEAAGLAKSMTIDTRGTMTTKKRK
                     TAGEDLAKQVSEAKQAALLKHTKQQIKEMQLSLFDIAPWPDTMRAMPNDTARSALFTTR
                     NKKIPREALQNKVIFHVNKDVKITYTGVELRADDDELVWQQVLEYAKRTPIGEPITFTF
                     YELCQDLGWSINGRYYTKAEECLSRLQATAMGFTSDRVGHLESVSLLHRFRVLDRGKKT
                     SRCQVLIDEEIVVLFAGDHYTKFIWEKYRKLSPTARRMFDYFSSHREPYPLKLETFRLM
                     CGSDSTRVKKWREQVGEACEELRGSGLVEHAWVNDDLVHCKR"
     gene            complement(7408..10376)
                     /gene="ssB"
                     /label=ssB
     CDS             complement(7408..7758)
                     /codon_start=1
                     /gene="ssB"
                     /product="single-stranded DNA binding protein"
                     /label=ssB
                     /protein_id="AGF38342.1"
                     /translation="MSHNQFQFIGNLTRDTEVRHGNSNKPQAIFDIAVNEEWRNDAGDK
                     QERTDFFRIKCFGSQAEAHGKYLGKGSLVFVQGKIRNTKYEKDGQTVYGTDFIADKVDY
                     LDTKAPGGSNQE"
     CDS             7933..8244
                     /codon_start=1
                     /gene="trbA"
                     /product="TrbA"
                     /label=trbA
                     /note="regulatory protein"
                     /protein_id="AGF38343.1"
                     /translation="MTKHELSERAGVSISFLSDLTNGKANPSLKVMEAIADALETPLPL
                     LLESTDLDREALAEIAGHPFKSSVPPGYERISVVLPSHKAFIVKKWGDDTRKKLRGRL"
     gene            7933..8244
                     /gene="trbA"
                     /label=trbA
     CDS             complement(9282..10376)
                     /codon_start=1
                     /gene="incC"
                     /product="plasmid maintenance protein"
                     /label=incC
                     /protein_id="AGF38344.1"
                     /translation="MGVIHEETAYRKPVPGGDPGAGSGAADHRDSAGRLSRWEATGDVR
                     NVAGTDQGRSVASGASRVGRVRGQELARGVRAGNGGSAGTSGVHRPEVGSGRQEKTGNQ
                     TMKTLVTANQKGGVGKTSTLVHLAFDFFERGLRVAVIDLDPQGNASYTLKDFATGLHAS
                     KLFGAVPAGGWTETAPAAGDGQAARLALIESNPVLANAERLSLDDARELFGANIKALAN
                     QGFDVCLIDTAPTLGVGLAAALFAADYVLSPIELEAYSIQGIKKMVTTIANVRQKNAKL
                     QFLGMVPSKVDARNPRHARHQAELLAAYPKMMIPATVGLRSSIADALASGVPVWKIKKT
                     AARKASKEVRALADYVFTKMEISQ"
     gene            complement(9282..10376)
                     /gene="incC"
                     /label=incC
     CDS             complement(10058..10363)
                     /codon_start=1
                     /gene="korA"
                     /product="KorA"
                     /label=korA
                     /note="regulatory protein"
                     /protein_id="AGF38345.1"
                     /translation="MKKRLTESQFQEAIQGLEVGQQTIEIARGVLVDGKPQATFATSLG
                     LTRGAVSQAVHRVWAAFEDKNLPEGYARVTAVLPEHQAYIVRKWEADAKKKQETKR"
     gene            complement(10058..10363)
                     /gene="korA"
                     /label=korA
     rep_origin      complement(10828..11539)
                     /direction=LEFT
                     /label=oriV
                     /note="incP origin of replication"
ORIGIN
        1 ctataaagat accaggcgtt tccccctgga agctccctcg tgcgctctcc tgttccgacc
       61 ctgccgctta ccggatacct gtccgccttt ctcccttcgg gaagcgtggc gccattcgcc
      121 attcaggctg cgcaactgtt gggaagggcg atcggtgcgg gcctcttcgc tattacgcca
      181 gctggcgaaa gggggatgtg ctgcaaggcg attaagttgg gtaacgccag ggttttccca
      241 gtcacgacgt tgtaaaacga cggccagtga attcccgggg atccgtcgac ctgcagccaa
      301 gcttggcgta atcatggtca tagctgtttc ctgtgtgaaa ttgttatccg ctcacaattc
      361 cacacaacat acgagccgga agcataaagt gtaaagcctg gggtgcctaa tgagtgagct
      421 aactcacatt aattgcgttg cgctcactgc ccgctttcca gtcgggaaac ctgtcgtgcc
      481 agctgcatta atgaatcggc caacgcgcgg ggagaggcgg tttgcgtatt gggcgctcgg
      541 tcttgccttg ctcgtcggtg atgtacttca ccagctccgc gaagtcgctc ttcttgatgg
      601 agcgcatggg gacgtgcttg gcaatcacgc gcaccccccg gccgttttag cggctaaaaa
      661 agtcatggct ctgccctcgg gcggaccacg cccatcatga ccttgccaag ctcgtcctgc
      721 ttctcttcga tcttcgccag cagggcgagg atcgtggcat caccgaaccg cgccgtgcgc
      781 gggtcgtcgg tgagccagag tttcagcagg ccgcccaggc ggcccaggtc gccattgatg
      841 cgggccagct cgcggacgtg ctcatagtcc acgacgcccg tgattttgta gccctggccg
      901 acggccagca ggtaggccga caggctcatg ccggccgccg ccgccttttc ctcaatcgct
      961 cttcgttcgt ctggaaggca gtacaccttg ataggtgggc tgcccttcct ggttggcttg
     1021 gtttcatcag ccatccgctt gccctcatct gttacgccgg cggtagccgg ccagcctcgc
     1081 agagcaggat tcccgttgag caccgccagg tgcgaataag ggacagtgaa gaaggaacac
     1141 ccgctcgcgg gtgggcctac ttcacctatc ctgcccggct gacgccgttg gatacaccaa
     1201 ggaaagtcta cacgaaccct ttggcaaaat cctgtatatc gtgcgaaaaa ggatggatat
     1261 accgaaaaaa tcgctataat gaccccgaag cagggttatg cagcggaaaa gcgccacgct
     1321 tcccgaaggg agaaaggcgg acaggtatcc ggtaagcggc agggtcggaa caggagagcg
     1381 cacgagggag cttccagggg gaaacgcctg gtatctttat agtcctgtcg ggtttcgcca
     1441 cctctgactt gagcgtcgat ttttgtgatg ctcgtcaggg gggcggagcc tatggaaaaa
     1501 cgccagcaac gcggcctttt tacggttcct ggccttttgc tggccttttg ctcacatgtt
     1561 ctttcctgcg ttatcccctg attctgtgga taaccgtatt accgcctttg agtgagctga
     1621 taccgctcgc cgcagccgaa cgaccgagcg cagcgagtca gtgagcgagg aagcggaaga
     1681 gcgccagaag gccgccagag aggccgagcg cggccgtgag gcttggacgc tagggcaggg
     1741 catgaaaaag cccgtagcgg gctgctacgg gcgtctgacg cggtggaaag ggggagggga
     1801 tgttgtctac atggctctgc tgtagtgagt gggttgcgct ccggcagcgg tcctgatcaa
     1861 tcgtcaccct ttctcggtcc ttcaacgttc ctgacaacga gcctcctttt cgccaatcca
     1921 tcgacaatca ccgcgagtcc ctgctcgaac gctgcgtccg gaccggcttc gtcgaaggcg
     1981 tctatcgcgg cccgcaacag cggcgagagc ggagcctgtt caacggtgcc gccgcgctcg
     2041 ccggcatcgc tgtcgccggc ctgctcctca agcacggccc caacagtgaa gtagctgatt
     2101 gtcatcagcg cattgacggc gtccccggcc gaaaaacccg cctcgcagag gaagcgaagc
     2161 tgcgcgtcgg ccgtttccat ctgcggtgcg cccggtcgcg tgccggcatg gatgcgcgcg
     2221 ccatcgcggt aggcgagcag cgcctgcctg aagctgcggg cattcccgat cagaaatgag
     2281 cgccagtcgt cgtcggctct cggcaccgaa tgcgtatgat tctccgccag catggcttcg
     2341 gccagtgcgt cgagcagcgc ccgcttgttc ctgaagtgcc agtaaagcgc cggctgctga
     2401 acccccaacc gttccgccag tttgcgtgtc gtcagaccgt ctacgccgac ctcgttcaac
     2461 aggtccaggg cggcacggat cactgtattc ggctgcaact ttgtcatgct tgacacttta
     2521 tcactgataa acataatatg tccaccaact tatcagtgat aaagaatccg cgcgttcaat
     2581 cggaccagcg gaggctggtc cggaggccag acatgaaacc caacataccc ctgatcgtaa
     2641 ttctgagcac tgtcgcgctc gacgctgtcg gcatcggcct gattatgccg gtgctgccgg
     2701 gcctcctgcg cgatctggtt cactcgaacg acgtcaccgc ccactatggc attctgctgg
     2761 cgctgtatgc gttggtgcaa tttgcctgcg cacctgtgct gggcgcgctg tcggatcgtt
     2821 tcgggcggcg gccaatcttg ctcgtctcgc tggccggcgc cactgtcgac tacgccatca
     2881 tggcgacagc gcctttcctt tgggttctct atatcgggcg gatcgtggcc ggcatcaccg
     2941 gggcgactgg ggcggtagcc ggcgcttata ttgccgatat cactgatggc gatgagcgcg
     3001 cgcggcactt cggcttcatg agcgcctgtt tcgggttcgg gatggtcgcg ggacctgtgc
     3061 tcggtgggct gatgggcggt ttctcccccc acgctccgtt cttcgccgcg gcagccttga
     3121 acggcctcaa tttcctgacg ggctgtttcc ttttgccgga gtcgcacaaa ggcgaacgcc
     3181 ggccgttacg ccgggaggct ctcaacccgc tcgcttcgtt ccggtgggcc cggggcatga
     3241 ccgtcgtcgc cgccctgatg gcggtcttct tcatcatgca acttgtcgga caggtgccgg
     3301 ccgcgctttg ggtcattttc ggcgaggatc gctttcactg ggacgcgacc acgatcggca
     3361 tttcgcttgc cgcatttggc attctgcatt cactcgccca ggcaatgatc accggccctg
     3421 tagccgcccg gctcggcgaa aggcgggcac tcatgctcgg aatgattgcc gacggcacag
     3481 gctacatcct gcttgccttc gcgacacggg gatggatggc gttcccgatc atggtcctgc
     3541 ttgcttcggg tggcatcgga atgccggcgc tgcaagcaat gttgtccagg caggtggatg
     3601 aggaacgtca ggggcagctg caaggctcac tggcggcgct caccagcctg acctcgatcg
     3661 tcggacccct cctcttcacg gcgatctatg cggcttctat aacaacgtgg aacgggtggg
     3721 catggattgc aggcgctgcc ctctacttgc tctgcctgcc ggcgctgcgt cgcgggcttt
     3781 ggagcggcgc agggcaacga gccgatcgct gatcgtggaa acgataggcc tatgccatgc
     3841 gggtcaaggc gacttccggc aagctatacg cgccctagga gtgcggttgg aacgttggcc
     3901 cagccagata ctcccgatca cgagcaggac gccgatgatt tgaagcgcac tcagcgtctg
     3961 atctgcctcg ctggcctgcc gcagttcttc aacctcccgg cgcagctttt cgttctcaat
     4021 ttcagcatcc ctttcggcat accattttat gacggcggca gagtcataaa gcacctcatt
     4081 acccttgcca ccgcctcgca gaacgggcat tccctgttcc tgccagttct gaatggtacg
     4141 gatactcgca ccgaaaatgt cagccagctg ctttttgttg acttccattg ttcattccac
     4201 ggacaaaaac agagaaagga aacgacagag gccaaaaagc tcgctttcag cacctgtcgt
     4261 ttcctttctt ttcagagggt attttaaata aaaacattaa gttatgacga agaagaacgg
     4321 aaacgcctta aaccggaaaa ttttcataaa tagcgaaaac ccgcgaggtc gccgccccgt
     4381 aacaaggcgg atcgccggaa aggacccgca aatgataata attatcaatt gcatactatc
     4441 gacggcactg ctgccagata acaccaccgg ggaaacattc catcatgatg gccgtgcgga
     4501 cataggaagc cagttcatcc atcgctttct tgtctgctgc catttgcttt gtgacatcca
     4561 gcgccgcaca ttcagcagcg tttttcagcg cgttttcgat caacgtttca atgttggtat
     4621 caacaccagg tttaactttg aacttatcgg cactgacggt taccttgttc tgcgctggct
     4681 catcacgctg gataccaagg ctgatgttgt agatattggt caccggctga ggtgtttcga
     4741 ttgccgctgc gtggatagca ccatttgcga tagcggcgtc cttgatgaat gacactccat
     4801 tgcgaataag ttcgaaggag acggtgtcac gaatgcgctg gtccagctcg tcgattgcct
     4861 tttgtgcagc agaggtatca atctcaacgc caagcgtcat cgaagcgcaa tattgctgct
     4921 caccaaaacg cgtattgacc aggtgttcaa cggcaaattt ctgcccttct gatgtcagaa
     4981 aggtaaagtg attttctttc tggtattcag ttgctgtgtg tctggtttca gcaaaaccaa
     5041 gctcgcgcaa ttcggctgtg ccagatttag aaggcagatc accagacagc aacgcgccac
     5101 ggaaaaacag cgcatacaga acatccgtcg ccgcgccgga caacgtgata attttatgac
     5161 ccatgattta tttcctttta gacgtgagcc tgtcgcacag caaagccgcc gaaagttaac
     5221 ggtttgccca ggctcacaac tgaaagactt tctacggtgt gcgcgtgcga tgcgcgtaga
     5281 agactgattt atcaacctgt ctttatatca ggattcatta cctgactatt tgtgggtaaa
     5341 gttcgtagtg cgctgatcgt gcaaaatgat tttagttggg aacagttcgc aactctgtcc
     5401 cataaaaatc agcatattcc catctatccc atatccagcg cattgaccat cgggatactg
     5461 aagggagatt ccatcatctc ttagaaagat caccatctct tttgtttcaa tttgcatata
     5521 gctacctgga ggatttatga atacaaggat tttcatggac tattaccatg agattgattt
     5581 tccatcttta ttcgcgagag cagtggaaag cgatgacgat gtgggtacta cattgcgcat
     5641 tcacctactt tgtgagcgca tggtcgaagc atggatatgc gcatgctgtg actgccaaga
     5701 tcatcacgag caataaaccc gccggcaacg cccgcagcag cataccggcg acccctcggc
     5761 ctcgctgttc gggctccacg aaaacgccgg acagatgcgc cttgtgagcg tccttggggc
     5821 cgtcctcctg tttgaagacc gacagcccaa tgatctcgcc gtcgatgtag gcgccgaatg
     5881 ccacggcatc tcgcaaccgt tcagcgaacg cctccatggg ctttttctcc tcgtgctcgt
     5941 aaacggaccc gaacatctct ggagctttct tcagggccga caatcggatc tcgcggaaat
     6001 cctgcacgtc ggccgctcca agccgtcgaa tctgagcctt aatcacaatt gtcaatttta
     6061 atcctctgtt tatcggcagt tcgtagagcg cgccgtgcgt cccgagcgat actgagcgaa
     6121 gcaagtgcgt cgagcagtgc ccgcttgttc ctgaaatgcc agtaaagcgc tggctgctga
     6181 acccccagcc ggaactgacc ccacaaggcc ctagcgtttg caatgcacca ggtcatcatt
     6241 gacccaggcg tgttccacca ggccgctgcc tcgcaactct tcgcaggctt cgccgacctg
     6301 ctcgcgccac ttcttcacgc gggtggaatc cgatccgcac atgaggcgga aggtttccag
     6361 cttgagcggg tacggctccc ggtgcgagct gaaatagtcg aacatccgtc gggccgtcgg
     6421 cgacagcttg cggtacttct cccatatgaa tttcgtgtag tggtcgccag caaacagcac
     6481 gacgatttcc tcgtcgatca ggacctggca acgggacgtt ttcttgccac ggtccaggac
     6541 gcggaagcgg tgcagcagcg acaccgattc caggtgccca acgcggtcgg acgtgaagcc
     6601 catcgccgtc gcctgtaggc gcgacaggca ttcctcggcc ttcgtgtaat accggccatt
     6661 gatcgaccag cccaggtcct ggcaaagctc gtagaacgtg aaggtgatcg gctcgccgat
     6721 aggggtgcgc ttcgcgtact ccaacacctg ctgccacacc agttcgtcat cgtcggcccg
     6781 cagctcgacg ccggtgtagg tgatcttcac gtccttgttg acgtggaaaa tgaccttgtt
     6841 ttgcagcgcc tcgcgcggga ttttcttgtt gcgcgtggtg aacagggcag agcgggccgt
     6901 gtcgtttggc atcgctcgca tcgtgtccgg ccacggcgca atatcgaaca aggaaagctg
     6961 catttccttg atctgctgct tcgtgtgttt cagcaacgcg gcctgcttgg cctcgctgac
     7021 ctgttttgcc aggtcctcgc cggcggtttt tcgcttcttg gtcgtcatag ttcctcgcgt
     7081 gtcgatggtc atcgacttcg ccaaacctgc cgcctcctgt tcgagacgac gcgaacgctc
     7141 cacggcggcc gatggcgcgg gcagggcagg gggagccagt tgcacgctgt cgcgctcgat
     7201 cttggccgta gcttgctgga ccatcgagcc gacggactgg aaggtttcgc ggggcgcacg
     7261 catgacggtg cggcttgcga tggtttcggc atcctcggcg gaaaaccccg cgtcgatcag
     7321 ttcttgcctg tatgccttcc ggtcaaacgt ccgattcatt caccctcctt gcgggattgc
     7381 cccgactcac gccggggcaa tgtgccctta ttcctgattt gacccgcctg gtgccttggt
     7441 gtccagataa tccaccttat cggcaatgaa gtcggtcccg tagaccgtct ggccgtcctt
     7501 ctcgtacttg gtattccgaa tcttgccctg cacgaatacc agcgacccct tgcccaaata
     7561 cttgccgtgg gcctcggcct gagagccaaa acacttgatg cggaagaagt cggtgcgctc
     7621 ctgcttgtcg ccggcatcgt tgcgccactc ttcattaacc gctatatcga aaattgcttg
     7681 cggcttgtta gaattgccat gacgtacctc ggtgtcacgg gtaagattac cgataaactg
     7741 gaactgatta tggctcatat cgaaagtctc cttgagaaag gagactctag tttagctaaa
     7801 cattggttcc gctgtcaaga actttagcgg ctaaaatttt gcgggccgcg accaaaggtg
     7861 cgaggggcgg cttccgctgt gtacaaccag atatttttca ccaacatcct tcgtctgctc
     7921 gatgagcggg gcatgacgaa acatgagctg tcggagaggg caggggtttc aatttcgttt
     7981 ttatcagact taaccaacgg taaggccaac ccctcgttga aggtgatgga ggccattgcc
     8041 gacgccctgg aaactcccct acctcttctc ctggagtcca ccgaccttga ccgcgaggca
     8101 ctcgcggaga ttgcgggtca tcctttcaag agcagcgtgc cgcccggata cgaacgcatc
     8161 agtgtggttt tgccgtcaca taaggcgttt atcgtaaaga aatggggcga cgacacccga
     8221 aaaaagctgc gtggaaggct ctgacgccaa gggttagggc ttgcacttcc ttctttagcc
     8281 gctaaaacgg ccccttctct gcgggccgtc ggctcgcgca tcatatcgac atcctcaacg
     8341 gaagccgtgc cgcgaatggc atcgggcggg tgcgctttga cagttgtttt ctatcagaac
     8401 ccctacgtcg tgcggttcga ttagctgttt gtcttgcagg ctaaacactt tcggtatatc
     8461 gtttgcctgt gcgataatgt tgctaatgat ttgttgcgta ggggttactg aaaagtgagc
     8521 gggaaagaag agtttcagac catcaaggag cgggccaagc gcaagctgga acgcgacatg
     8581 ggtgcggacc tgttggccgc gctcaacgac ccgaaaaccg ttgaagtcat gctcaacgcg
     8641 gacggcaagg tgtggcacga acgccttggc gagccgatgc ggtacatctg cgacatgcgg
     8701 cccagccagt cgcaggcgat tatagaaacg gtggccggat tccacggcaa agaggtcacg
     8761 cggcattcgc ccatcctgga aggcgagttc cccttggatg gcagccgctt tgccggccaa
     8821 ttgccgccgg tcgtggccgc gccaaccttt gcgatccgca agcgcgcggt cgccatcttc
     8881 acgctggaac agtacgtcga ggcgggcatc atgacccgcg agcaatacga ggtcattaaa
     8941 agcgccgtga ttgatgatat agcggcccgg ctgctcctgg ttctcgcgca ccgaaatggg
     9001 tgacttcacc ccgcgctctt tgatcgtggc accgatttcc gcgatgctct ccggggaaaa
     9061 gccggggttg tcggccgtcc gcggctgatg cggatcttcg tcgatcaggt ccaggtccag
     9121 ctcgataggg ccggaaccgc cctgagacgc cgcaggagcg tccaggaggc tcgacaggtc
     9181 gccgatgcta tccaacccca ggccggacgg ctgcgccgcg cctgcggctt cctgagcggc
     9241 cgcagcggtg tttttcttgg tggtcttggc ttgagccgca gtcattggga aatctccatc
     9301 ttcgtgaaca cgtaatcagc cagggcgcga acctctttcg atgccttgcg cgcggccgtt
     9361 ttcttgatct tccagaccgg cacaccggat gcgagggcat cggcgatgct gctgcgcagg
     9421 ccaacggtgg ccggaatcat catcttgggg tacgcggcca gcagctcggc ttggtggcgc
     9481 gcgtggcgcg gattccgcgc atcgaccttg ctgggcacca tgccaaggaa ttgcagcttg
     9541 gcgttcttct ggcgcacgtt cgcaatggtc gtgaccatct tcttgatgcc ctggatgctg
     9601 tacgcctcaa gctcgatggg ggacagcaca tagtcggccg cgaagagggc ggccgccagg
     9661 ccgacgccaa gggtcggggc cgtgtcgatc aggcacacgt cgaagccttg gttcgccagg
     9721 gccttgatgt tcgccccgaa cagctcgcgg gcgtcgtcca gcgacagccg ttcggcgttc
     9781 gccagtaccg ggttggactc gatgagggcg aggcgcgcgg cctggccgtc gccggctgcg
     9841 ggtgcggttt cggtccagcc gccggcaggg acagcgccga acagcttgct tgcatgcagg
     9901 ccggtagcaa agtccttgag cgtgtaggac gcattgccct gggggtccag gtcgatcacg
     9961 gcaacccgca agccgcgctc gaaaaagtcg aaggcaagat gcacaagggt cgaagtcttg
    10021 ccgacgccgc ctttctggtt ggccgtgacc aaagttttca tcgtttggtt tcctgttttt
    10081 tcttggcgtc cgcttcccac ttccggacga tgtacgcctg atgttccggc agaaccgccg
    10141 ttacccgcgc gtacccctcg ggcaagttct tgtcctcgaa cgcggcccac acgcgatgca
    10201 ccgcttgcga cactgcgccc ctggtcagtc ccagcgacgt tgcgaacgtc gcctgtggct
    10261 tcccatcgac taagacgccc cgcgctatct cgatggtctg ctgccccact tccagcccct
    10321 ggatcgcctc ctggaactgg ctttcggtaa gccgtttctt catggataac acccataatt
    10381 tgctccgcgc cttggttgaa catagcggtg acagccgcca gcacatgaga gaagtttagc
    10441 taaacatttc tcgcacgtca acacctttag ccgctaaaac tcgtccttgg cgtaacaaaa
    10501 caaaagcccg gaaaccgggc tttcgtctct tgccgcttat ggctctgcac ccggctccat
    10561 caccaacagg tcgcgcacgc gcttcactcg gttgcggatc gacactgcca gcccaacaaa
    10621 gccggttgcc gccgccgcca ggatcgcgcc gatgatgccg gccacaccgg ccatcgccca
    10681 ccaggtcgcc gccctcactg cccggcacct ggtcgctgaa tgtcgatgcc agcacctgcg
    10741 gcacgtcaat gcttccgggc gtcgcgctcg ggctgatcgc ccatcccgtt actgccccga
    10801 tcccggcaat ggcaaggact gccagcgctg ccatttttgg ggtgaggccg ttcgcggccg
    10861 aggggcgcag cccctggggg gatgggaggc ccgcgttagc gggccgggag ggttcgagaa
    10921 gggggggcac cccccttcgg cgtgcgcggt cacgcgcaca gggcgcagcc ctggttaaaa
    10981 acaaggttta taaatattgg tttaaaagca ggttaaaaga caggttagcg gtggccgaaa
    11041 aacgggcgga aacccttgca aatgctggat tttctgcctg tggacagccc ctcaaatgtc
    11101 aataggtgcg cccctcatct gtcagcactc tgcccctcaa gtgtcaagga tcgcgcccct
    11161 catctgtcag tagtcgcgcc cctcaagtgt caataccgca gggcacttat ccccaggctt
    11221 gtccacatca tctgtgggaa actcgcgtaa aatcaggcgt tttcgccgat ttgcgaggct
    11281 ggccagctcc acgtcgccgg ccgaaatcga gcctgcccct catctgtcaa cgccgcgccg
    11341 ggtgagtcgg cccctcaagt gtcaacgtcc gcccctcatc tgtcagtgag ggccaagttt
    11401 tccgcgaggt atccacaacg ccggcggccg cggtgtctcg cacacggctt cgacggcgtt
    11461 tctggcgcgt ttgcagggcc atagacggcc gccagcccag cggcgagggc aaccagcccg
    11521 gtgagcgtcg gaaaggcgct cttccgcttc ctcgctcact gactcgctgc gctcggtcgt
    11581 tcggctgcgg cgagcggtat cagctcactc aaaggcggta atacggttat ccacagaatc
    11641 aggggataac gcaggaaaga acatgtgagc aaaaggccag caaaaggcca ggaaccgtaa
    11701 aaaggccgcg ttgctggcgt ttttccatag gctccgcccc cctgacgagc atcacaaaaa
    11761 tcgacgctca agtcagaggt ggcgaaaccc gacagga
//
